| Gene Symbol | VPS53 |
| Entrez Gene ID | 55275 |
| Full Name | VPS53, GARP complex subunit |
| Synonyms | HCCS1,PCH2E,hVps53L,pp13624 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: | |
| Disorder Html: | Pontocerebellar hypoplasia, type 2E, 615851 (3) |
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VPS53 cDNA ORF clone, Homo sapiens(human)
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Gene
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Clones
gRNAs
The following VPS53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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Reference Sequences (Refseq)
CloneID OHu107092 Clone ID Related Accession (Same CDS sequence) NM_001366253.2 , NM_001366253.1 Accession Version NM_001366253.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector 
User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1570550400000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 3 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. On May 31, 2019 this sequence version replaced NM_001366253.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.52831.1, SRR1660807.163452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001353182.1 CDS 131..2230 Translation 
Target ORF information:
RefSeq Version NM_001366253.2 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK 
NM_001366253.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu107092 Clone ID Related Accession (Same CDS sequence) NM_001366253.2 , NM_001366253.1 Accession Version NM_001366253.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2100bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1551196800000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 3 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY444797.1, SRR1660805.52831.1 [ECO:0000332] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001353182.1 CDS 131..2230 Translation
Target ORF information:
RefSeq Version NM_001366253.1 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001366253.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGGTGAG ATGGACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu28566 Clone ID Related Accession (Same CDS sequence) NM_001128159.2 , NM_001128159.3 Accession Version NM_001128159.2 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1506787200000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AL701573.1, EU021218.1, AK001841.1 and AC015853.8. This sequence is a reference standard in the RefSeqGene project. On Aug 11, 2010 this sequence version replaced NM_001128159.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.155068.1, SRR1803617.254618.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001121631.1 CDS 148..2646 Translation
Target ORF information:
RefSeq Version NM_001128159.2 Organism Homo sapiens(human) Definition Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001128159.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu28566 Clone ID Related Accession (Same CDS sequence) NM_001128159.2 , NM_001128159.3 Accession Version NM_001128159.3 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1570550400000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 1 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AL701573.1, EU021218.1, AK001841.1 and AC015853.8. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001128159.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.155068.1, SRR1803617.254618.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000437048.7/ ENSP00000401435.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001121631.1 CDS 131..2629 Translation
Target ORF information:
RefSeq Version NM_001128159.3 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 1, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001128159.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGCGCT TGAAGAGGCT
CAGAAAGCTA TCCAACAACT CTTTGGCAAA ATCAAAGATA TCAAAGACAA AGCTGAAAAA
TCAGAGCAAA TGGTGAAAGA AATCACCCGT GATATTAAGC AATTAGATCA CGCCAAACGC
CACCTGACCA CCTCAATCAC CACACTGAAC CACCTGCACA TGCTGGCAGG AGGTGTCGAC
TCCCTCGAAG CCATGACCAG GCGAAGACAA TACGGAGAAG TTGCTAATCT CCTTCAGGGT
GTGATGAATG TCCTGGAGCA CTTCCACAAG TATATGGGGA TTCCGCAGAT CCGGCAGCTT
TCCGAAAGAG TGAAGGCTGC ACAGACTGAG TTAGGACAGC AAATCCTGGC AGATTTTGAA
GAAGCGTTTC CTTCCCAGGG CACCAAGAGA CCAGGAGGAC CCAGCAATGT TCTACGAGAT
GCATGTCTGG TTGCTAATAT TCTAGATCCC AGGATCAAAC AGGAAATCAT CAAAAAGTTT
ATTAAACAGC ATCTGTCAGA GTATCTGGTA CTTTTTCAAG AAAACCAAGA TGTTGCCTGG
CTGGACAAAA TCGACAGACG CTATGCCTGG ATAAAACGCC AGCTTGTGGA CTATGAGGAG
AAATACGGCC GCATGTTTCC ACGTGAGTGG TGCATGGCTG AGAGGATTGC GGTGGAATTT
TGCCATGTGA CAAGGGCAGA ACTTGCCAAG ATTATGCGTA CCAGAGCGAA GGAAATTGAA
GTGAAATTGC TTCTTTTTGC TATTCAAAGA ACAACTAACT TTGAGGGGTT TCTTGCAAAA
CGCTTCTCCG GCTGCACCCT GACCGATGGG ACCCTGAAAA AGCTTGAGTC TCCACCCCCA
TCTACCAATC CCTTCCTGGA AGATGAGCCA ACACCAGAGA TGGAGGAACT GGCAACGGAG
AAAGGAGATT TAGATCAACC AAAGAAGCCT AAAGCCCCAG ACAATCCATT TCATGGCATT
GTTTCCAAGT GTTTTGAGCC TCATCTCTAC GTGTATATCG AATCCCAAGA CAAGAACCTC
GGAGAGCTGA TAGATCGGTT TGTGGCTGAT TTCAAAGCCC AGGGGCCACC TAAGCCCAAC
ACTGATGAAG GGGGTGCCGT GCTCCCCAGC TGCGCCGACC TCTTTGTCTA CTACAAGAAG
TGCATGGTGC AATGCTCTCA GCTCAGTACT GGGGAGCCCA TGATCGCCCT GACCACCATT
TTCCAGAAGT ACCTCCGAGA ATACGCCTGG AAAATCCTCT CTGGCAACCT GCCCAAAACC
ACAACCAGCA GTGGAGGACT GACTATCAGC AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA
GTAGCCAAGT TCACTCTGGA GGAGCTCTGC CTCATCTGTA ACATCCTGAG CACGGCAGAG
TACTGTCTGG CCACCACCCA GCAGCTAGAA GAAAAACTCA AAGAAAAAGT GGATGTAAGT
CTGATTGAAC GAATCAATCT GACTGGAGAG ATGGACACGT TCAGCACCGT CATCTCCAGC
AGTATTCAGC TGCTGGTTCA GGATCTGGAT GCTGCCTGTG ATCCTGCCCT GACTGCCATG
AGCAAGATGC AGTGGCAGAA CGTGGAGCAC GTTGGTGACC AGAGCCCCTA CGTCACCTCT
GTCATTCTGC ACATCAAGCA GAACGTCCCC ATCATCCGTG ACAACCTGGC TTCCACACGC
AAGTACTTCA CTCAGTTCTG CGTTAAATTT GCAAACTCCT TCATTCCCAA ATTCATCACC
CACCTCTTCA AGTGCAAGCC AATTAGCATG GTGGGAGCAG AACAGCTGCT GCTGGACACC
CACTCGCTGA AGATGGTCCT GCTCGATCTC CCCTCCATCA GCTCGCAGGT GGTGAGGAAG
GCACCCGCCA GCTACACCAA GATCGTTGTC AAAGGCATGA CCCGGGCTGA GATGATCCTC
AAGGTAGTGA TGGCCCCTCA TGAACCGTTG GTGGTGTTTG TTGACAACTA CATCAAACTT
CTCACAGACT GCAACACAGA AACCTTTCAG AAGATACTGG ACATGAAGGG GCTGAAGAGG
AGTGAGCAGA GCAGCATGCT GGAACTCCTG CGCCAGCGGC TCCCCGCACC GCCCTCGGGG
GCAGAAAGCT CCGGCTCACT GTCCCTGACG GCGCCAACAC CAGAGCAAGA GTCGTCACGC
ATCCGCAAGC TCGAGAAACT CATTAAAAAG AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu28642 Clone ID Related Accession (Same CDS sequence) NM_018289.4 , NM_018289.3 Accession Version NM_018289.4 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2013bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1570636800000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 2 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AK290343.1, AK001841.1 and AC015853.8. On May 31, 2019 this sequence version replaced NM_018289.3. Transcript Variant: This variant (2) has multiple differences in the coding region and uses a different segment for its 3' UTR but maintains the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290343.1, BC006116.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_060759.2 CDS 131..2143 Translation
Target ORF information:
RefSeq Version NM_018289.4 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_018289.4
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu28642 Clone ID Related Accession (Same CDS sequence) NM_018289.4 , NM_018289.3 Accession Version NM_018289.3 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2013bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1548518400000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 2 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092532.1, AK290343.1 and AK001841.1. On Jun 8, 2008 this sequence version replaced NM_018289.2. Transcript Variant: This variant (2) has multiple differences in the coding region and uses a different segment for its 3' UTR but maintains the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290343.1, BC006116.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_060759.2 CDS 148..2160 Translation
Target ORF information:
RefSeq Version NM_018289.3 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_018289.3
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981ATGATGGAGG AGGAGGAACT GGAGTTCGTG GAGGAGCTGG AAGCCGTGCT GCAGCTCACG
CCCGAGGTGC AGCTGGCCAT CGAGCAGGTG TTTCCAAGCC AGGACCCTCT AGATCGAGCA
GATTTCAATG CTGTTGAGTA TATCAATACC CTGTTCCCAA CCGAGCAATC TCTGGCGAAC
ATAGACGAAG TCGTGAACAA AATTAGGCTG AAAATAAGGA GACTGGATGA CAATATTCGA
ACTGTTGTAA GAGGTCAGAC GAACGTGGGG CAGGATGGAC GGCAAGTGAA AGAAATCACC
CGTGATATTA AGCAATTAGA TCACGCCAAA CGCCACCTGA CCACCTCAAT CACCACACTG
AACCACCTGC ACATGCTGGC AGGAGGTGTC GACTCCCTCG AAGCCATGAC CAGGCGAAGA
CAATACGGAG AAGTTGCTAA TCTCCTTCAG GGTGTGATGA ATGTCCTGGA GCACTTCCAC
AAGTATATGG GGATTCCGCA GATCCGGCAG CTTTCCGAAA GAGTGAAGGC TGCACAGACT
GAGTTAGGAC AGCAAATCCT GGCAGATTTT GAAGAAGCGT TTCCTTCCCA GGGCACCAAG
AGACCAGGAG GACCCAGCAA TGTTCTACGA GATGCATGTC TGGTTGCTAA TATTCTAGAT
CCCAGGATCA AACAGGAAAT CATCAAAAAG TTTATTAAAC AGCATCTGTC AGAGTATCTG
GTACTTTTTC AAGAAAACCA AGATGTTGCC TGGCTGGACA AAATCGACAG ACGCTATGCC
TGGATAAAAC GCCAGCTTGT GGACTATGAG GAGAAATACG GCCGCATGTT TCCACGTGAG
TGGTGCATGG CTGAGAGGAT TGCGGTGGAA TTTTGCCATG TGACAAGGGC AGAACTTGCC
AAGATTATGC GTACCAGAGC GAAGGAAATT GAAGTGAAAT TGCTTCTTTT TGCTATTCAA
AGAACAACTA ACTTTGAGGG GTTTCTTGCA AAACGCTTCT CCGGCTGCAC CCTGACCGAT
GGGACCCTGA AAAAGCTTGA GTCTCCACCC CCATCTACCA ATCCCTTCCT GGAAGATGAG
CCAACACCAG AGATGGAGGA ACTGGCAACG GAGAAAGGAG ATTTAGATCA ACCAAAGAAG
CCTAAAGCCC CAGACAATCC ATTTCATGGC ATTGTTTCCA AGTGTTTTGA GCCTCATCTC
TACGTGTATA TCGAATCCCA AGACAAGAAC CTCGGAGAGC TGATAGATCG GTTTGTGGCT
GATTTCAAAG CCCAGGGGCC ACCTAAGCCC AACACTGATG AAGGGGGTGC CGTGCTCCCC
AGCTGCGCCG ACCTCTTTGT CTACTACAAG AAGTGCATGG TGCAATGCTC TCAGCTCAGT
ACTGGGGAGC CCATGATCGC CCTGACCACC ATTTTCCAGA AGTACCTCCG AGAATACGCC
TGGAAAATCC TCTCTGGCAA CCTGCCCAAA ACCACAACCA GCAGTGGAGG ACTGACTATC
AGCAGCCTCC TCAAGGAAAA GGAGGGCTCA GAAGTAGCCA AGTTCACTCT GGAGGAGCTC
TGCCTCATCT GTAACATCCT GAGCACGGCA GAGTACTGTC TGGCCACCAC CCAGCAGCTA
GAAGAAAAAC TCAAAGAAAA AGTGGATGTA AGTCTGATTG AACGAATCAA TCTGACTGGA
GAGATGGACA CGTTCAGCAC CGTCATCTCC AGCAGTATTC AGCTGCTGGT TCAGGATCTG
GATGCTGCCT GTGATCCTGC CCTGACTGCC ATGAGCAAGA TGCAGTGGCA GAACGTGGAG
CACGTTGGTG ACCAGAGCCC CTACGTCACC TCTGTCATTC TGCACATCAA GCAGAACGTC
CCCATCATCC GTGACAACCT GGCTTCCACA CGCAAGTACT TCACTCAGTT CTGCGTTAAA
TTTGCAAACT CCTTCATTCC CAAATTCATC ACCCACCTCT TCAAGTGCAA GCCAATTAGC
ATGGTGGGAG CAGAACAGGT GAGATGGACG TAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu69264 Clone ID Related Accession (Same CDS sequence) NM_001366254.1 , NM_001366254.2 , XM_011523953.2 Accession Version NM_001366254.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1548518400000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 4 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. On Sep 27, 2018 this sequence version replaced XM_011523953.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092532.1 [ECO:0000332] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG
ACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001353183.1 CDS 823..2328 Translation
Target ORF information:
RefSeq Version NM_001366254.1 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001366254.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG
ACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu69264 Clone ID Related Accession (Same CDS sequence) NM_001366254.1 , NM_001366254.2 , XM_011523953.2 Accession Version NM_001366254.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1570636800000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform 4 Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC027455.22 and AC015853.8. On May 31, 2019 this sequence version replaced NM_001366254.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK092532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG
ACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq NP_001353183.1 CDS 823..2328 Translation
Target ORF information:
RefSeq Version NM_001366254.2 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant 4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK NM_001366254.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG
ACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu69264 Clone ID Related Accession (Same CDS sequence) NM_001366254.1 , NM_001366254.2 , XM_011523953.2 Accession Version XM_011523953.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform X3 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG
ACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_011522255.1 CDS 830..2335 Translation
Target ORF information:
RefSeq Version XM_011523953.2 Organism Homo sapiens(human) Definition Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X4, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_011523953.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501ATGTTCTCAC TCATAGTGAA GGCTGCACAG ACTGAGTTAG GACAGCAAAT CCTGGCAGAT
TTTGAAGAAG CGTTTCCTTC CCAGGGCACC AAGAGACCAG GAGGACCCAG CAATGTTCTA
CGAGATGCAT GTCTGGTTGC TAATATTCTA GATCCCAGGA TCAAACAGGA AATCATCAAA
AAGTTTATTA AACAGCATCT GTCAGAGTAT CTGGTACTTT TTCAAGAAAA CCAAGATGTT
GCCTGGCTGG ACAAAATCGA CAGACGCTAT GCCTGGATAA AACGCCAGCT TGTGGACTAT
GAGGAGAAAT ACGGCCGCAT GTTTCCACGT GAGTGGTGCA TGGCTGAGAG GATTGCGGTG
GAATTTTGCC ATGTGACAAG GGCAGAACTT GCCAAGATTA TGCGTACCAG AGCGAAGGAA
ATTGAAGTGA AATTGCTTCT TTTTGCTATT CAAAGAACAA CTAACTTTGA GGGGTTTCTT
GCAAAACGCT TCTCCGGCTG CACCCTGACC GATGGGACCC TGAAAAAGCT TGAGTCTCCA
CCCCCATCTA CCAATCCCTT CCTGGAAGAT GAGCCAACAC CAGAGATGGA GGAACTGGCA
ACGGAGAAAG GAGATTTAGA TCAACCAAAG AAGCCTAAAG CCCCAGACAA TCCATTTCAT
GGCATTGTTT CCAAGTGTTT TGAGCCTCAT CTCTACGTGT ATATCGAATC CCAAGACAAG
AACCTCGGAG AGCTGATAGA TCGGTTTGTG GCTGATTTCA AAGCCCAGGG GCCACCTAAG
CCCAACACTG ATGAAGGGGG TGCCGTGCTC CCCAGCTGCG CCGACCTCTT TGTCTACTAC
AAGAAGTGCA TGGTGCAATG CTCTCAGCTC AGTACTGGGG AGCCCATGAT CGCCCTGACC
ACCATTTTCC AGAAGTACCT CCGAGAATAC GCCTGGAAAA TCCTCTCTGG CAACCTGCCC
AAAACCACAA CCAGCAGTGG AGGACTGACT ATCAGCAGCC TCCTCAAGGA AAAGGAGGGC
TCAGAAGTAG CCAAGTTCAC TCTGGAGGAG CTCTGCCTCA TCTGTAACAT CCTGAGCACG
GCAGAGTACT GTCTGGCCAC CACCCAGCAG CTAGAAGAAA AACTCAAAGA AAAAGTGGAT
GTAAGTCTGA TTGAACGAAT CAATCTGACT GGAGAGATGG ACACGTTCAG CACCGTCATC
TCCAGCAGTA TTCAGCTGCT GGTTCAGGAT CTGGATGCTG CCTGTGATCC TGCCCTGACT
GCCATGAGCA AGATGCAGTG GCAGAACGTG GAGCACGTTG GTGACCAGAG CCCCTACGTC
ACCTCTGTCA TTCTGCACAT CAAGCAGAAC GTCCCCATCA TCCGTGACAA CCTGGCTTCC
ACACGCAAGT ACTTCACTCA GTTCTGCGTT AAATTTGCAA ACTCCTTCAT TCCCAAATTC
ATCACCCACC TCTTCAAGTG CAAGCCAATT AGCATGGTGG GAGCAGAACA GGTGAGATGG
ACGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu86964 Clone ID Related Accession (Same CDS sequence) XM_017024817.1 , XM_017024817.2 Accession Version XM_017024817.1 Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1465056000000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG
AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016880306.1 CDS 344..2692 Translation
Target ORF information:
RefSeq Version XM_017024817.1 Organism Homo sapiens(human) Definition Homo sapiens VPS53, GARP complex subunit (VPS53), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017024817.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG
AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu86964 Clone ID Related Accession (Same CDS sequence) XM_017024817.1 , XM_017024817.2 Accession Version XM_017024817.2 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform X1 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG
AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016880306.1 CDS 344..2692 Translation
Target ORF information:
RefSeq Version XM_017024817.2 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X2, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017024817.2
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341ATGGATGGGA ATTTGGCTTT CTGTCTTCAT GGAAGAACTT GGCCTCCTTT TTCCTCTTCC
CTCTTTCATC CACGGAAACA GTATTTAGAA GAGCACAGAT TCAGCGTTGT GGTTGTGCCG
TCACCATTTC CATGTGCGCT TGAAGAGGCT CAGAAAGCTA TCCAACAACT CTTTGGCAAA
ATCAAAGATA TCAAAGACAA AGCTGAAAAA TCAGAGCAAA TGGTGAAAGA AATCACCCGT
GATATTAAGC AATTAGATCA CGCCAAACGC CACCTGACCA CCTCAATCAC CACACTGAAC
CACCTGCACA TGCTGGCAGG AGGTGTCGAC TCCCTCGAAG CCATGACCAG GCGAAGACAA
TACGGAGAAG TTGCTAATCT CCTTCAGGGT GTGATGAATG TCCTGGAGCA CTTCCACAAG
TATATGGGGA TTCCGCAGAT CCGGCAGCTT TCCGAAAGAG TGAAGGCTGC ACAGACTGAG
TTAGGACAGC AAATCCTGGC AGATTTTGAA GAAGCGTTTC CTTCCCAGGG CACCAAGAGA
CCAGGAGGAC CCAGCAATGT TCTACGAGAT GCATGTCTGG TTGCTAATAT TCTAGATCCC
AGGATCAAAC AGGAAATCAT CAAAAAGTTT ATTAAACAGC ATCTGTCAGA GTATCTGGTA
CTTTTTCAAG AAAACCAAGA TGTTGCCTGG CTGGACAAAA TCGACAGACG CTATGCCTGG
ATAAAACGCC AGCTTGTGGA CTATGAGGAG AAATACGGCC GCATGTTTCC ACGTGAGTGG
TGCATGGCTG AGAGGATTGC GGTGGAATTT TGCCATGTGA CAAGGGCAGA ACTTGCCAAG
ATTATGCGTA CCAGAGCGAA GGAAATTGAA GTGAAATTGC TTCTTTTTGC TATTCAAAGA
ACAACTAACT TTGAGGGGTT TCTTGCAAAA CGCTTCTCCG GCTGCACCCT GACCGATGGG
ACCCTGAAAA AGCTTGAGTC TCCACCCCCA TCTACCAATC CCTTCCTGGA AGATGAGCCA
ACACCAGAGA TGGAGGAACT GGCAACGGAG AAAGGAGATT TAGATCAACC AAAGAAGCCT
AAAGCCCCAG ACAATCCATT TCATGGCATT GTTTCCAAGT GTTTTGAGCC TCATCTCTAC
GTGTATATCG AATCCCAAGA CAAGAACCTC GGAGAGCTGA TAGATCGGTT TGTGGCTGAT
TTCAAAGCCC AGGGGCCACC TAAGCCCAAC ACTGATGAAG GGGGTGCCGT GCTCCCCAGC
TGCGCCGACC TCTTTGTCTA CTACAAGAAG TGCATGGTGC AATGCTCTCA GCTCAGTACT
GGGGAGCCCA TGATCGCCCT GACCACCATT TTCCAGAAGT ACCTCCGAGA ATACGCCTGG
AAAATCCTCT CTGGCAACCT GCCCAAAACC ACAACCAGCA GTGGAGGACT GACTATCAGC
AGCCTCCTCA AGGAAAAGGA GGGCTCAGAA GTAGCCAAGT TCACTCTGGA GGAGCTCTGC
CTCATCTGTA ACATCCTGAG CACGGCAGAG TACTGTCTGG CCACCACCCA GCAGCTAGAA
GAAAAACTCA AAGAAAAAGT GGATGTAAGT CTGATTGAAC GAATCAATCT GACTGGAGAG
ATGGACACGT TCAGCACCGT CATCTCCAGC AGTATTCAGC TGCTGGTTCA GGATCTGGAT
GCTGCCTGTG ATCCTGCCCT GACTGCCATG AGCAAGATGC AGTGGCAGAA CGTGGAGCAC
GTTGGTGACC AGAGCCCCTA CGTCACCTCT GTCATTCTGC ACATCAAGCA GAACGTCCCC
ATCATCCGTG ACAACCTGGC TTCCACACGC AAGTACTTCA CTCAGTTCTG CGTTAAATTT
GCAAACTCCT TCATTCCCAA ATTCATCACC CACCTCTTCA AGTGCAAGCC AATTAGCATG
GTGGGAGCAG AACAGCTGCT GCTGGACACC CACTCGCTGA AGATGGTCCT GCTCGATCTC
CCCTCCATCA GCTCGCAGGT GGTGAGGAAG GCACCCGCCA GCTACACCAA GATCGTTGTC
AAAGGCATGA CCCGGGCTGA GATGATCCTC AAGGTAGTGA TGGCCCCTCA TGAACCGTTG
GTGGTGTTTG TTGACAACTA CATCAAACTT CTCACAGACT GCAACACAGA AACCTTTCAG
AAGATACTGG ACATGAAGGG GCTGAAGAGG AGTGAGCAGA GCAGCATGCT GGAACTCCTG
CGCCAGCGGC TCCCCGCACC GCCCTCGGGG GCAGAAAGCT CCGGCTCACT GTCCCTGACG
GCGCCAACAC CAGAGCAAGA GTCGTCACGC ATCCGCAAGC TCGAGAAACT CATTAAAAAG
AGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID OHu86965 Clone ID Related Accession (Same CDS sequence) XM_017024818.1 Accession Version XM_017024818.1 Latest version! Documents for ORF clone product in default vector Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
Protein sequence
SNPVector pcDNA3.1-C-(k)DYK or customized vector User ManualClone information Clone Map MSDSTag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags ORF Insert Method CloneEZ™ Seamless cloning technology Insert Structure linear Update Date 1575475200000 Organism Homo sapiens(human) Product vacuolar protein sorting-associated protein 53 homolog isoform X2 Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101ATGGTGAAAG AAATCACCCG TGATATTAAG CAATTAGATC ACGCCAAACG CCACCTGACC
ACCTCAATCA CCACACTGAA CCACCTGCAC ATGCTGGCAG GAGGTGTCGA CTCCCTCGAA
GCCATGACCA GGCGAAGACA ATACGGAGAA GTTGCTAATC TCCTTCAGGG TGTGATGAAT
GTCCTGGAGC ACTTCCACAA GTATATGGGG ATTCCGCAGA TCCGGCAGCT TTCCGAAAGA
GTGAAGGCTG CACAGACTGA GTTAGGACAG CAAATCCTGG CAGATTTTGA AGAAGCGTTT
CCTTCCCAGG GCACCAAGAG ACCAGGAGGA CCCAGCAATG TTCTACGAGA TGCATGTCTG
GTTGCTAATA TTCTAGATCC CAGGATCAAA CAGGAAATCA TCAAAAAGTT TATTAAACAG
CATCTGTCAG AGTATCTGGT ACTTTTTCAA GAAAACCAAG ATGTTGCCTG GCTGGACAAA
ATCGACAGAC GCTATGCCTG GATAAAACGC CAGCTTGTGG ACTATGAGGA GAAATACGGC
CGCATGTTTC CACGTGAGTG GTGCATGGCT GAGAGGATTG CGGTGGAATT TTGCCATGTG
ACAAGGGCAG AACTTGCCAA GATTATGCGT ACCAGAGCGA AGGAAATTGA AGTGAAATTG
CTTCTTTTTG CTATTCAAAG AACAACTAAC TTTGAGGGGT TTCTTGCAAA ACGCTTCTCC
GGCTGCACCC TGACCGATGG GACCCTGAAA AAGCTTGAGT CTCCACCCCC ATCTACCAAT
CCCTTCCTGG AAGATGAGCC AACACCAGAG ATGGAGGAAC TGGCAACGGA GAAAGGAGAT
TTAGATCAAC CAAAGAAGCC TAAAGCCCCA GACAATCCAT TTCATGGCAT TGTTTCCAAG
TGTTTTGAGC CTCATCTCTA CGTGTATATC GAATCCCAAG ACAAGAACCT CGGAGAGCTG
ATAGATCGGT TTGTGGCTGA TTTCAAAGCC CAGGGGCCAC CTAAGCCCAA CACTGATGAA
GGGGGTGCCG TGCTCCCCAG CTGCGCCGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG
CAATGCTCTC AGCTCAGTAC TGGGGAGCCC ATGATCGCCC TGACCACCAT TTTCCAGAAG
TACCTCCGAG AATACGCCTG GAAAATCCTC TCTGGCAACC TGCCCAAAAC CACAACCAGC
AGTGGAGGAC TGACTATCAG CAGCCTCCTC AAGGAAAAGG AGGGCTCAGA AGTAGCCAAG
TTCACTCTGG AGGAGCTCTG CCTCATCTGT AACATCCTGA GCACGGCAGA GTACTGTCTG
GCCACCACCC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TGGATGTAAG TCTGATTGAA
CGAATCAATC TGACTGGAGA GATGGACACG TTCAGCACCG TCATCTCCAG CAGTATTCAG
CTGCTGGTTC AGGATCTGGA TGCTGCCTGT GATCCTGCCC TGACTGCCAT GAGCAAGATG
CAGTGGCAGA ACGTGGAGCA CGTTGGTGAC CAGAGCCCCT ACGTCACCTC TGTCATTCTG
CACATCAAGC AGAACGTCCC CATCATCCGT GACAACCTGG CTTCCACACG CAAGTACTTC
ACTCAGTTCT GCGTTAAATT TGCAAACTCC TTCATTCCCA AATTCATCAC CCACCTCTTC
AAGTGCAAGC CAATTAGCAT GGTGGGAGCA GAACAGCTGC TGCTGGACAC CCACTCGCTG
AAGATGGTCC TGCTCGATCT CCCCTCCATC AGCTCGCAGG TGGTGAGGAA GGCACCCGCC
AGCTACACCA AGATCGTTGT CAAAGGCATG ACCCGGGCTG AGATGATCCT CAAGGTAGTG
ATGGCCCCTC ATGAACCGTT GGTGGTGTTT GTTGACAACT ACATCAAACT TCTCACAGAC
TGCAACACAG AAACCTTTCA GAAGATACTG GACATGAAGG GGCTGAAGAG GAGTGAGCAG
AGCAGCATGC TGGAACTCCT GCGCCAGCGG CTCCCCGCAC CGCCCTCGGG GGCAGAAAGC
TCCGGCTCAC TGTCCCTGAC GGCGCCAACA CCAGAGCAAG AGTCGTCACG CATCCGCAAG
CTCGAGAAAC TCATTAAAAA GAGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq XP_016880307.1 CDS 758..2887 Translation
Target ORF information:
RefSeq Version XM_017024818.1 Organism Homo sapiens(human) Definition Homo sapiens VPS53 subunit of GARP complex (VPS53), transcript variant X3, mRNA. Target ORF information:
Epitope DYKDDDDK Bacterial selection AMPR Mammalian selection NeoR Vector pcDNA3.1+/C-(K)DYK XM_017024818.1
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101ATGGTGAAAG AAATCACCCG TGATATTAAG CAATTAGATC ACGCCAAACG CCACCTGACC
ACCTCAATCA CCACACTGAA CCACCTGCAC ATGCTGGCAG GAGGTGTCGA CTCCCTCGAA
GCCATGACCA GGCGAAGACA ATACGGAGAA GTTGCTAATC TCCTTCAGGG TGTGATGAAT
GTCCTGGAGC ACTTCCACAA GTATATGGGG ATTCCGCAGA TCCGGCAGCT TTCCGAAAGA
GTGAAGGCTG CACAGACTGA GTTAGGACAG CAAATCCTGG CAGATTTTGA AGAAGCGTTT
CCTTCCCAGG GCACCAAGAG ACCAGGAGGA CCCAGCAATG TTCTACGAGA TGCATGTCTG
GTTGCTAATA TTCTAGATCC CAGGATCAAA CAGGAAATCA TCAAAAAGTT TATTAAACAG
CATCTGTCAG AGTATCTGGT ACTTTTTCAA GAAAACCAAG ATGTTGCCTG GCTGGACAAA
ATCGACAGAC GCTATGCCTG GATAAAACGC CAGCTTGTGG ACTATGAGGA GAAATACGGC
CGCATGTTTC CACGTGAGTG GTGCATGGCT GAGAGGATTG CGGTGGAATT TTGCCATGTG
ACAAGGGCAG AACTTGCCAA GATTATGCGT ACCAGAGCGA AGGAAATTGA AGTGAAATTG
CTTCTTTTTG CTATTCAAAG AACAACTAAC TTTGAGGGGT TTCTTGCAAA ACGCTTCTCC
GGCTGCACCC TGACCGATGG GACCCTGAAA AAGCTTGAGT CTCCACCCCC ATCTACCAAT
CCCTTCCTGG AAGATGAGCC AACACCAGAG ATGGAGGAAC TGGCAACGGA GAAAGGAGAT
TTAGATCAAC CAAAGAAGCC TAAAGCCCCA GACAATCCAT TTCATGGCAT TGTTTCCAAG
TGTTTTGAGC CTCATCTCTA CGTGTATATC GAATCCCAAG ACAAGAACCT CGGAGAGCTG
ATAGATCGGT TTGTGGCTGA TTTCAAAGCC CAGGGGCCAC CTAAGCCCAA CACTGATGAA
GGGGGTGCCG TGCTCCCCAG CTGCGCCGAC CTCTTTGTCT ACTACAAGAA GTGCATGGTG
CAATGCTCTC AGCTCAGTAC TGGGGAGCCC ATGATCGCCC TGACCACCAT TTTCCAGAAG
TACCTCCGAG AATACGCCTG GAAAATCCTC TCTGGCAACC TGCCCAAAAC CACAACCAGC
AGTGGAGGAC TGACTATCAG CAGCCTCCTC AAGGAAAAGG AGGGCTCAGA AGTAGCCAAG
TTCACTCTGG AGGAGCTCTG CCTCATCTGT AACATCCTGA GCACGGCAGA GTACTGTCTG
GCCACCACCC AGCAGCTAGA AGAAAAACTC AAAGAAAAAG TGGATGTAAG TCTGATTGAA
CGAATCAATC TGACTGGAGA GATGGACACG TTCAGCACCG TCATCTCCAG CAGTATTCAG
CTGCTGGTTC AGGATCTGGA TGCTGCCTGT GATCCTGCCC TGACTGCCAT GAGCAAGATG
CAGTGGCAGA ACGTGGAGCA CGTTGGTGAC CAGAGCCCCT ACGTCACCTC TGTCATTCTG
CACATCAAGC AGAACGTCCC CATCATCCGT GACAACCTGG CTTCCACACG CAAGTACTTC
ACTCAGTTCT GCGTTAAATT TGCAAACTCC TTCATTCCCA AATTCATCAC CCACCTCTTC
AAGTGCAAGC CAATTAGCAT GGTGGGAGCA GAACAGCTGC TGCTGGACAC CCACTCGCTG
AAGATGGTCC TGCTCGATCT CCCCTCCATC AGCTCGCAGG TGGTGAGGAA GGCACCCGCC
AGCTACACCA AGATCGTTGT CAAAGGCATG ACCCGGGCTG AGATGATCCT CAAGGTAGTG
ATGGCCCCTC ATGAACCGTT GGTGGTGTTT GTTGACAACT ACATCAAACT TCTCACAGAC
TGCAACACAG AAACCTTTCA GAAGATACTG GACATGAAGG GGCTGAAGAG GAGTGAGCAG
AGCAGCATGC TGGAACTCCT GCGCCAGCGG CTCCCCGCAC CGCCCTCGGG GGCAGAAAGC
TCCGGCTCAC TGTCCCTGAC GGCGCCAACA CCAGAGCAAG AGTCGTCACG CATCCGCAAG
CTCGAGAAAC TCATTAAAAA GAGACTGTAGThe stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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Hepatocellular carcinoma suppressor 1 promoter hypermethylation in serum. A diagnostic and prognostic study in hepatitis B.
Clinics and research in hepatology and gastroenterology41(2)171-180(2017 Mar)
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EARP is a multisubunit tethering complex involved in endocytic recycling.
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Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array.
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