NADSYN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NADSYN1
Entrez Gene ID	55191
Full Name	NAD synthetase 1
General protein information	Preferred Names NAD synthetase 1 Names glutamine-dependent NAD(+) synthetase NAD(+) synthase NAD(+) synthetase glutamine-dependent NAD synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.4
Summary	Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD).[supplied by OMIM, Apr 2004].
Disorder MIM:	608285

mRNA and Protein(s)

mRNA	Protein	Name
NM_018161.5	NP_060631.2	glutamine-dependent NAD(+) synthetase
NM_018161.4	NP_060631.2	glutamine-dependent NAD(+) synthetase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NADSYN1	XP_001174076.2
Drosophila melanogaster (fruit fly)	CG9940	NP_727727.1
Saccharomyces cerevisiae (baker's yeast)	QNS1	NP_011941.1
Macaca mulatta (Rhesus monkey)	NADSYN1	XP_001098992.2
Canis lupus familiaris (dog)	NADSYN1	XP_540795.4
Gallus gallus (chicken)	NADSYN1	NP_001006465.1
Arabidopsis thaliana (thale cress)	AT1G55090	NP_175906.1
Oryza sativa (rice)	Os07g0167100	NP_001058975.1
Eremothecium gossypii	AGOS_AGR358W	NP_987024.1
Neurospora crassa	NCU04648	XP_959191.1
Schizosaccharomyces pombe (fission yeast)	SPCC553.02	NP_587771.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000112	XP_311035.4
Bos taurus (cattle)	NADSYN1	NP_001029615.1
Rattus norvegicus (Norway rat)	Nadsyn1	NP_852145.1
Kluyveromyces lactis	KLLA0D13024g	XP_453643.1
Homo sapiens (human)	NADSYN1	NP_060631.2
Mus musculus (house mouse)	Nadsyn1	NP_084497.1
Danio rerio (zebrafish)	nadsyn1	NP_001092723.1
Xenopus tropicalis (tropical clawed frog)	nadsyn1	NP_001120406.1

Gene Ontology
Bibliography

Related articles in PubMed

Vitamin D Deficiency in Uygurs and Kazaks Is Associated with Polymorphisms in CYP2R1 and DHCR7/NADSYN1 Genes.
Xu X, Mao J, Zhang M, Liu H, Li H, Lei H, Han L, Gao M
Medical science monitor : international medical journal of experimental and clinical research211960-8(2015 Jul)

Enrichment of vitamin D response elements in RA-associated loci supports a role for vitamin D in the pathogenesis of RA.
Yarwood A, Martin P, Bowes J, Lunt M, Worthington J, Barton A, Eyre S
Genes and immunity14(5)325-9(2013 Jul-Aug)

Effect of polymorphisms in the NADSYN1/DHCR7 locus (rs12785878 and rs1790349) on plasma 25-hydroxyvitamin D levels and coronary artery disease incidence.
Abu El Maaty MA, Hassanein SI, Sleem HM, Gad MZ
Journal of nutrigenetics and nutrigenomics6(6)327-35(2013)

Associations between polymorphisms related to calcium metabolism and human height: the Tromsø Study.
Jorde R, Svartberg J, Joakimsen RM, Grimnes G
Annals of human genetics76(3)200-10(2012 May)

Association of vitamin D-related gene polymorphisms with manifestation of vitamin D deficiency in children.
Kitanaka S, Isojima T, Takaki M, Numakura C, Hayasaka K, Igarashi T
Endocrine journal59(11)1007-14(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NADSYN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NADSYN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02157	NM_018161.5 Latest version!	Homo sapiens NAD synthetase 1 (NADSYN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu02157	NM_018161.4	Homo sapiens NAD synthetase 1 (NADSYN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NADSYN1 ( NM_018161.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060631.2 Homo sapiens NAD synthetase 1 (NADSYN1), mRNA.

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CloneID	OHu02157
Clone ID Related Accession (Same CDS sequence)	NM_018161.5 , NM_018161.4
Accession Version	NM_018161.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	glutamine-dependent NAD(+) synthetase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI460954.1, AK022436.1, BE730392.1, BC020977.1, BM554316.1, AW804480.1 and AP000867.5. On May 17, 2019 this sequence version replaced NM_018161.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK022436.1, BC003638.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000319023.7/ ENSP00000326424.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGGCCGGA AGGTGACCGT GGCCACCTGC GCACTCAACC AGTGGGCCCT GGACTTCGAG 
GGCAATTTGC AAAGAATTTT AAAGAGTATT GAAATTGCCA AAAACAGAGG AGCAAGATAC 
AGGCTTGGAC CAGAGCTGGA AATATGCGGC TACGGATGTT GGGATCATTA TTACGAGTCG 
GACACCCTCT TGCACTCGTT TCAAGTCCTA GCGGCCCTTG TGGAGTCTCC CGTCACTCAG 
GACATCATCT GCGACGTGGG GATGCCTGTA ATGCACCGAA ACGTCCGCTA CAACTGCAGA 
GTGATATTCC TCAACAGGAA GATCCTGCTC ATCAGACCCA AGATGGCCTT GGCCAATGAA 
GGCAACTACC GCGAGCTGCG CTGGTTCACC CCGTGGTCGA GGAGTCGGCA CACAGAGGAG 
TACTTTCTGC CTCGGATGAT ACAGGACCTG ACAAAGCAGG AAACCGTACC CTTCGGAGAT 
GCGGTGCTGG TGACATGGGA CACCTGCATT GGAAGTGAGA TCTGTGAGGA GCTCTGGACA 
CCCCACAGCC CGCACATCGA CATGGGCCTG GATGGCGTGG AGATCATCAC CAACGCCTCG 
GGCAGCCACC AAGTGCTGCG CAAAGCCAAC ACCAGGGTGG ATCTCGTGAC TATGGTCACC 
AGCAAGAACG GTGGGATTTA CTTGCTGGCC AACCAGAAGG GTTGTGACGG GGACCGCCTG 
TACTACGACG GCTGTGCCAT GATTGCCATG AACGGAAGCG TCTTTGCTCA AGGATCCCAG 
TTTTCTCTGG ATGACGTGGA AGTCCTGACG GCCACGCTGG ATCTGGAGGA CGTCCGGAGC 
TACAGGGCGG AGATTTCATC TCGAAACCTG GCGGCCAGCA GGGCGAGCCC CTACCCCAGA 
GTGAAGGTGG ACTTTGCCCT CTCGTGCCAC GAGGACTTGC TGGCACCCAT CTCTGAGCCC 
ATCGAGTGGA AATACCACAG CCCTGAGGAG GAGATAAGCC TTGGACCTGC CTGCTGGCTC 
TGGGATTTTT TAAGACGAAG TCAACAGGCA GGGTTTTTGC TGCCCTTGAG TGGCGGGGTG 
GACAGCGCAG CCACCGCCTG CCTCATCTAC TCCATGTGCT GCCAGGTCTG CGAGGCCGTG 
AGGAGTGGAA ATGAGGAAGT GCTGGCTGAT GTCCGCACCA TCGTGAACCA GATCAGCTAC 
ACCCCCCAGG ATCCCCGAGA CCTCTGTGGA CGCATACTGA CCACCTGCTA CATGGCCAGC 
AAGAACTCCT CCCAGGAGAC GTGCACCCGG GCCAGAGAGT TGGCCCAGCA GATTGGAAGC 
CACCACATCA GTCTCAACAT CGATCCAGCC GTGAAGGCCG TCATGGGCAT CTTCAGCCTG 
GTGACGGGGA AGAGCCCTCT GTTTGCAGCT CATGGAGGAA GCAGCAGGGA AAACCTGGCG 
CTGCAAAATG TGCAGGCTCG AATACGGATG GTCCTCGCCT ATCTGTTTGC TCAGTTGAGC 
CTCTGGTCTC GGGGTGTCCA CGGTGGGCTC CTCGTGCTGG GATCCGCCAA CGTGGATGAG 
AGTCTCCTGG GCTACCTGAC CAAGTACGAC TGCTCCAGTG CGGACATCAA CCCCATAGGC 
GGGATCAGCA AGACGGACCT CAGGGCCTTC GTCCAGTTCT GCATCCAGCG CTTCCAGCTT 
CCTGCCCTGC AGAGCATCCT GTTGGCGCCG GCCACCGCAG AGCTGGAGCC CTTGGCTGAT 
GGACAGGTGT CCCAGACCGA CGAGGAAGAT ATGGGGATGA CATATGCGGA GCTCTCGGTC 
TATGGGAAAC TCAGGAAGGT GGCCAAGATG GGGCCCTACA GCATGTTCTG CAAACTCCTC 
GGCATGTGGA GACACATCTG CACCCCGAGA CAGGTCGCTG ACAAAGTGAA GCGGTTTTTC 
TCCAAGTACT CCATGAACAG ACACAAGATG ACCACGCTCA CACCCGCGTA CCACGCCGAG 
AACTACAGCC CTGAGGACAA CAGGTTTGAT CTGCGACCAT TTCTGTACAA CACAAGCTGG 
CCTTGGCAGT TTCGGTGCAT AGAAAATCAG GTGCTACAGC TCGAGAGGGC AGAGCCACAG 
TCCCTGGACG GCGTGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060631.2
CDS	95..2215
Translation	MGRKVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESPVTQDIICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEEYFLPRMIQDLTKQETVPFGDAVLVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRVKVDFALSCHEDLLAPISEPIEWKYHSPEEEISLGPACWLWDFLRRSQQAGFLLPLSGGVDSAATACLIYSMCCQVCEAVRSGNEEVLADVRTIVNQISYTPQDPRDLCGRILTTCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLALQNVQARIRMVLAYLFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIQRFQLPALQSILLAPATAELEPLADGQVSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSMFCKLLGMWRHICTPRQVADKVKRFFSKYSMNRHKMTTLTPAYHAENYSPEDNRFDLRPFLYNTSWPWQFRCIENQVLQLERAEPQSLDGVD

Target ORF information:

RefSeq Version	NM_018161.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NAD synthetase 1 (NADSYN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018161.5

ORF Insert Sequence:

ATGGGCCGGA AGGTGACCGT GGCCACCTGC GCACTCAACC AGTGGGCCCT GGACTTCGAG 
GGCAATTTGC AAAGAATTTT AAAGAGTATT GAAATTGCCA AAAACAGAGG AGCAAGATAC 
AGGCTTGGAC CAGAGCTGGA AATATGCGGC TACGGATGTT GGGATCATTA TTACGAGTCG 
GACACCCTCT TGCACTCGTT TCAAGTCCTA GCGGCCCTTG TGGAGTCTCC CGTCACTCAG 
GACATCATCT GCGACGTGGG GATGCCTGTA ATGCACCGAA ACGTCCGCTA CAACTGCAGA 
GTGATATTCC TCAACAGGAA GATCCTGCTC ATCAGACCCA AGATGGCCTT GGCCAATGAA 
GGCAACTACC GCGAGCTGCG CTGGTTCACC CCGTGGTCGA GGAGTCGGCA CACAGAGGAG 
TACTTTCTGC CTCGGATGAT ACAGGACCTG ACAAAGCAGG AAACCGTACC CTTCGGAGAT 
GCGGTGCTGG TGACATGGGA CACCTGCATT GGAAGTGAGA TCTGTGAGGA GCTCTGGACA 
CCCCACAGCC CGCACATCGA CATGGGCCTG GATGGCGTGG AGATCATCAC CAACGCCTCG 
GGCAGCCACC AAGTGCTGCG CAAAGCCAAC ACCAGGGTGG ATCTCGTGAC TATGGTCACC 
AGCAAGAACG GTGGGATTTA CTTGCTGGCC AACCAGAAGG GTTGTGACGG GGACCGCCTG 
TACTACGACG GCTGTGCCAT GATTGCCATG AACGGAAGCG TCTTTGCTCA AGGATCCCAG 
TTTTCTCTGG ATGACGTGGA AGTCCTGACG GCCACGCTGG ATCTGGAGGA CGTCCGGAGC 
TACAGGGCGG AGATTTCATC TCGAAACCTG GCGGCCAGCA GGGCGAGCCC CTACCCCAGA 
GTGAAGGTGG ACTTTGCCCT CTCGTGCCAC GAGGACTTGC TGGCACCCAT CTCTGAGCCC 
ATCGAGTGGA AATACCACAG CCCTGAGGAG GAGATAAGCC TTGGACCTGC CTGCTGGCTC 
TGGGATTTTT TAAGACGAAG TCAACAGGCA GGGTTTTTGC TGCCCTTGAG TGGCGGGGTG 
GACAGCGCAG CCACCGCCTG CCTCATCTAC TCCATGTGCT GCCAGGTCTG CGAGGCCGTG 
AGGAGTGGAA ATGAGGAAGT GCTGGCTGAT GTCCGCACCA TCGTGAACCA GATCAGCTAC 
ACCCCCCAGG ATCCCCGAGA CCTCTGTGGA CGCATACTGA CCACCTGCTA CATGGCCAGC 
AAGAACTCCT CCCAGGAGAC GTGCACCCGG GCCAGAGAGT TGGCCCAGCA GATTGGAAGC 
CACCACATCA GTCTCAACAT CGATCCAGCC GTGAAGGCCG TCATGGGCAT CTTCAGCCTG 
GTGACGGGGA AGAGCCCTCT GTTTGCAGCT CATGGAGGAA GCAGCAGGGA AAACCTGGCG 
CTGCAAAATG TGCAGGCTCG AATACGGATG GTCCTCGCCT ATCTGTTTGC TCAGTTGAGC 
CTCTGGTCTC GGGGTGTCCA CGGTGGGCTC CTCGTGCTGG GATCCGCCAA CGTGGATGAG 
AGTCTCCTGG GCTACCTGAC CAAGTACGAC TGCTCCAGTG CGGACATCAA CCCCATAGGC 
GGGATCAGCA AGACGGACCT CAGGGCCTTC GTCCAGTTCT GCATCCAGCG CTTCCAGCTT 
CCTGCCCTGC AGAGCATCCT GTTGGCGCCG GCCACCGCAG AGCTGGAGCC CTTGGCTGAT 
GGACAGGTGT CCCAGACCGA CGAGGAAGAT ATGGGGATGA CATATGCGGA GCTCTCGGTC 
TATGGGAAAC TCAGGAAGGT GGCCAAGATG GGGCCCTACA GCATGTTCTG CAAACTCCTC 
GGCATGTGGA GACACATCTG CACCCCGAGA CAGGTCGCTG ACAAAGTGAA GCGGTTTTTC 
TCCAAGTACT CCATGAACAG ACACAAGATG ACCACGCTCA CACCCGCGTA CCACGCCGAG 
AACTACAGCC CTGAGGACAA CAGGTTTGAT CTGCGACCAT TTCTGTACAA CACAAGCTGG 
CCTTGGCAGT TTCGGTGCAT AGAAAATCAG GTGCTACAGC TCGAGAGGGC AGAGCCACAG 
TCCCTGGACG GCGTGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NADSYN1 ( NM_018161.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060631.2 Homo sapiens NAD synthetase 1 (NADSYN1), mRNA.

Price & Availability↑

CloneID	OHu02157
Clone ID Related Accession (Same CDS sequence)	NM_018161.5 , NM_018161.4
Accession Version	NM_018161.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529596800000
Organism	Homo sapiens(human)
Product	glutamine-dependent NAD(+) synthetase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB141878.1, BI460954.1, AK022436.1, BE730392.1, BC020977.1, BM554316.1, AW804480.1 and BC003638.2. On Jan 30, 2004 this sequence version replaced NM_018161.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003638.2, SRR1163657.317563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCGGA AGGTGACCGT GGCCACCTGC GCACTCAACC AGTGGGCCCT GGACTTCGAG 
GGCAATTTGC AAAGAATTTT AAAGAGTATT GAAATTGCCA AAAACAGAGG AGCAAGATAC 
AGGCTTGGAC CAGAGCTGGA AATATGCGGC TACGGATGTT GGGATCATTA TTACGAGTCG 
GACACCCTCT TGCACTCGTT TCAAGTCCTA GCGGCCCTTG TGGAGTCTCC CGTCACTCAG 
GACATCATCT GCGACGTGGG GATGCCTGTA ATGCACCGAA ACGTCCGCTA CAACTGCAGA 
GTGATATTCC TCAACAGGAA GATCCTGCTC ATCAGACCCA AGATGGCCTT GGCCAATGAA 
GGCAACTACC GCGAGCTGCG CTGGTTCACC CCGTGGTCGA GGAGTCGGCA CACAGAGGAG 
TACTTTCTGC CTCGGATGAT ACAGGACCTG ACAAAGCAGG AAACCGTACC CTTCGGAGAT 
GCGGTGCTGG TGACATGGGA CACCTGCATT GGAAGTGAGA TCTGTGAGGA GCTCTGGACA 
CCCCACAGCC CGCACATCGA CATGGGCCTG GATGGCGTGG AGATCATCAC CAACGCCTCG 
GGCAGCCACC AAGTGCTGCG CAAAGCCAAC ACCAGGGTGG ATCTCGTGAC TATGGTCACC 
AGCAAGAACG GTGGGATTTA CTTGCTGGCC AACCAGAAGG GTTGTGACGG GGACCGCCTG 
TACTACGACG GCTGTGCCAT GATTGCCATG AACGGAAGCG TCTTTGCTCA AGGATCCCAG 
TTTTCTCTGG ATGACGTGGA AGTCCTGACG GCCACGCTGG ATCTGGAGGA CGTCCGGAGC 
TACAGGGCGG AGATTTCATC TCGAAACCTG GCGGCCAGCA GGGCGAGCCC CTACCCCAGA 
GTGAAGGTGG ACTTTGCCCT CTCGTGCCAC GAGGACTTGC TGGCACCCAT CTCTGAGCCC 
ATCGAGTGGA AATACCACAG CCCTGAGGAG GAGATAAGCC TTGGACCTGC CTGCTGGCTC 
TGGGATTTTT TAAGACGAAG TCAACAGGCA GGGTTTTTGC TGCCCTTGAG TGGCGGGGTG 
GACAGCGCAG CCACCGCCTG CCTCATCTAC TCCATGTGCT GCCAGGTCTG CGAGGCCGTG 
AGGAGTGGAA ATGAGGAAGT GCTGGCTGAT GTCCGCACCA TCGTGAACCA GATCAGCTAC 
ACCCCCCAGG ATCCCCGAGA CCTCTGTGGA CGCATACTGA CCACCTGCTA CATGGCCAGC 
AAGAACTCCT CCCAGGAGAC GTGCACCCGG GCCAGAGAGT TGGCCCAGCA GATTGGAAGC 
CACCACATCA GTCTCAACAT CGATCCAGCC GTGAAGGCCG TCATGGGCAT CTTCAGCCTG 
GTGACGGGGA AGAGCCCTCT GTTTGCAGCT CATGGAGGAA GCAGCAGGGA AAACCTGGCG 
CTGCAAAATG TGCAGGCTCG AATACGGATG GTCCTCGCCT ATCTGTTTGC TCAGTTGAGC 
CTCTGGTCTC GGGGTGTCCA CGGTGGGCTC CTCGTGCTGG GATCCGCCAA CGTGGATGAG 
AGTCTCCTGG GCTACCTGAC CAAGTACGAC TGCTCCAGTG CGGACATCAA CCCCATAGGC 
GGGATCAGCA AGACGGACCT CAGGGCCTTC GTCCAGTTCT GCATCCAGCG CTTCCAGCTT 
CCTGCCCTGC AGAGCATCCT GTTGGCGCCG GCCACCGCAG AGCTGGAGCC CTTGGCTGAT 
GGACAGGTGT CCCAGACCGA CGAGGAAGAT ATGGGGATGA CATATGCGGA GCTCTCGGTC 
TATGGGAAAC TCAGGAAGGT GGCCAAGATG GGGCCCTACA GCATGTTCTG CAAACTCCTC 
GGCATGTGGA GACACATCTG CACCCCGAGA CAGGTCGCTG ACAAAGTGAA GCGGTTTTTC 
TCCAAGTACT CCATGAACAG ACACAAGATG ACCACGCTCA CACCCGCGTA CCACGCCGAG 
AACTACAGCC CTGAGGACAA CAGGTTTGAT CTGCGACCAT TTCTGTACAA CACAAGCTGG 
CCTTGGCAGT TTCGGTGCAT AGAAAATCAG GTGCTACAGC TCGAGAGGGC AGAGCCACAG 
TCCCTGGACG GCGTGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060631.2
CDS	127..2247
Translation	MGRKVTVATCALNQWALDFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESPVTQDIICDVGMPVMHRNVRYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSRSRHTEEYFLPRMIQDLTKQETVPFGDAVLVTWDTCIGSEICEELWTPHSPHIDMGLDGVEIITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSVFAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRVKVDFALSCHEDLLAPISEPIEWKYHSPEEEISLGPACWLWDFLRRSQQAGFLLPLSGGVDSAATACLIYSMCCQVCEAVRSGNEEVLADVRTIVNQISYTPQDPRDLCGRILTTCYMASKNSSQETCTRARELAQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLALQNVQARIRMVLAYLFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFCIQRFQLPALQSILLAPATAELEPLADGQVSQTDEEDMGMTYAELSVYGKLRKVAKMGPYSMFCKLLGMWRHICTPRQVADKVKRFFSKYSMNRHKMTTLTPAYHAENYSPEDNRFDLRPFLYNTSWPWQFRCIENQVLQLERAEPQSLDGVD

Target ORF information:

RefSeq Version	NM_018161.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NAD synthetase 1 (NADSYN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018161.4

ORF Insert Sequence:

ATGGGCCGGA AGGTGACCGT GGCCACCTGC GCACTCAACC AGTGGGCCCT GGACTTCGAG 
GGCAATTTGC AAAGAATTTT AAAGAGTATT GAAATTGCCA AAAACAGAGG AGCAAGATAC 
AGGCTTGGAC CAGAGCTGGA AATATGCGGC TACGGATGTT GGGATCATTA TTACGAGTCG 
GACACCCTCT TGCACTCGTT TCAAGTCCTA GCGGCCCTTG TGGAGTCTCC CGTCACTCAG 
GACATCATCT GCGACGTGGG GATGCCTGTA ATGCACCGAA ACGTCCGCTA CAACTGCAGA 
GTGATATTCC TCAACAGGAA GATCCTGCTC ATCAGACCCA AGATGGCCTT GGCCAATGAA 
GGCAACTACC GCGAGCTGCG CTGGTTCACC CCGTGGTCGA GGAGTCGGCA CACAGAGGAG 
TACTTTCTGC CTCGGATGAT ACAGGACCTG ACAAAGCAGG AAACCGTACC CTTCGGAGAT 
GCGGTGCTGG TGACATGGGA CACCTGCATT GGAAGTGAGA TCTGTGAGGA GCTCTGGACA 
CCCCACAGCC CGCACATCGA CATGGGCCTG GATGGCGTGG AGATCATCAC CAACGCCTCG 
GGCAGCCACC AAGTGCTGCG CAAAGCCAAC ACCAGGGTGG ATCTCGTGAC TATGGTCACC 
AGCAAGAACG GTGGGATTTA CTTGCTGGCC AACCAGAAGG GTTGTGACGG GGACCGCCTG 
TACTACGACG GCTGTGCCAT GATTGCCATG AACGGAAGCG TCTTTGCTCA AGGATCCCAG 
TTTTCTCTGG ATGACGTGGA AGTCCTGACG GCCACGCTGG ATCTGGAGGA CGTCCGGAGC 
TACAGGGCGG AGATTTCATC TCGAAACCTG GCGGCCAGCA GGGCGAGCCC CTACCCCAGA 
GTGAAGGTGG ACTTTGCCCT CTCGTGCCAC GAGGACTTGC TGGCACCCAT CTCTGAGCCC 
ATCGAGTGGA AATACCACAG CCCTGAGGAG GAGATAAGCC TTGGACCTGC CTGCTGGCTC 
TGGGATTTTT TAAGACGAAG TCAACAGGCA GGGTTTTTGC TGCCCTTGAG TGGCGGGGTG 
GACAGCGCAG CCACCGCCTG CCTCATCTAC TCCATGTGCT GCCAGGTCTG CGAGGCCGTG 
AGGAGTGGAA ATGAGGAAGT GCTGGCTGAT GTCCGCACCA TCGTGAACCA GATCAGCTAC 
ACCCCCCAGG ATCCCCGAGA CCTCTGTGGA CGCATACTGA CCACCTGCTA CATGGCCAGC 
AAGAACTCCT CCCAGGAGAC GTGCACCCGG GCCAGAGAGT TGGCCCAGCA GATTGGAAGC 
CACCACATCA GTCTCAACAT CGATCCAGCC GTGAAGGCCG TCATGGGCAT CTTCAGCCTG 
GTGACGGGGA AGAGCCCTCT GTTTGCAGCT CATGGAGGAA GCAGCAGGGA AAACCTGGCG 
CTGCAAAATG TGCAGGCTCG AATACGGATG GTCCTCGCCT ATCTGTTTGC TCAGTTGAGC 
CTCTGGTCTC GGGGTGTCCA CGGTGGGCTC CTCGTGCTGG GATCCGCCAA CGTGGATGAG 
AGTCTCCTGG GCTACCTGAC CAAGTACGAC TGCTCCAGTG CGGACATCAA CCCCATAGGC 
GGGATCAGCA AGACGGACCT CAGGGCCTTC GTCCAGTTCT GCATCCAGCG CTTCCAGCTT 
CCTGCCCTGC AGAGCATCCT GTTGGCGCCG GCCACCGCAG AGCTGGAGCC CTTGGCTGAT 
GGACAGGTGT CCCAGACCGA CGAGGAAGAT ATGGGGATGA CATATGCGGA GCTCTCGGTC 
TATGGGAAAC TCAGGAAGGT GGCCAAGATG GGGCCCTACA GCATGTTCTG CAAACTCCTC 
GGCATGTGGA GACACATCTG CACCCCGAGA CAGGTCGCTG ACAAAGTGAA GCGGTTTTTC 
TCCAAGTACT CCATGAACAG ACACAAGATG ACCACGCTCA CACCCGCGTA CCACGCCGAG 
AACTACAGCC CTGAGGACAA CAGGTTTGAT CTGCGACCAT TTCTGTACAA CACAAGCTGG 
CCTTGGCAGT TTCGGTGCAT AGAAAATCAG GTGCTACAGC TCGAGAGGGC AGAGCCACAG 
TCCCTGGACG GCGTGGACTG A