WIPI1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	WIPI1
Entrez Gene ID	55062
Full Name	WD repeat domain, phosphoinositide interacting 1
Synonyms	ATG18,ATG18A,WIPI49
General protein information	Preferred Names WD repeat domain, phosphoinositide interacting 1 Names WD repeat domain phosphoinositide-interacting protein 1 WIPI-1 alpha atg18 protein homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q24.2
Summary	This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016].
Disorder MIM:	609224

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320772.1	NP_001307701.1	WD repeat domain phosphoinositide-interacting protein 1 isoform b
XM_017024808.1	XP_016880297.1	WD repeat domain phosphoinositide-interacting protein 1 isoform X1
NM_017983.6	NP_060453.3	WD repeat domain phosphoinositide-interacting protein 1 isoform a
NM_017983.7	NP_060453.3	WD repeat domain phosphoinositide-interacting protein 1 isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	WIPI1	XP_548021.3
Bos taurus (cattle)	WIPI1	NP_001092510.1
Homo sapiens (human)	WIPI1	NP_060453.3
Pan troglodytes (chimpanzee)	WIPI1	XP_001165276.1
Rattus norvegicus (Norway rat)	Wipi1	NP_001120769.1
Danio rerio (zebrafish)	wipi1	NP_956685.1
Mus musculus (house mouse)	Wipi1	NP_666052.1
Gallus gallus (chicken)	WIPI1	XP_415688.4
Xenopus tropicalis (tropical clawed frog)	wipi1	NP_001072161.1

Gene Ontology
Bibliography

Related articles in PubMed

WIPI1, BAG1, and PEX3 Autophagy-Related Genes Are Relevant Melanoma Markers.
D'Arcangelo D, Giampietri C, Muscio M, Scatozza F, Facchiano F, Facchiano A
Oxidative medicine and cellular longevity20181471682(2018)

WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.
Bakula D, Müller AJ, Zuleger T, Takacs Z, Franz-Wachtel M, Thost AK, Brigger D, Tschan MP, Frickey T, Robenek H, Macek B, Proikas-Cezanne T
Nature communications815637(2017 05)

Fluorescence-based imaging of autophagy progression by human WIPI protein detection.
Thost AK, Dönnes P, Kohlbacher O, Proikas-Cezanne T
Methods (San Diego, Calif.)7569-78(2015 Mar)

WIPI β-propellers at the crossroads of autophagosome and lipid droplet dynamics.
Pfisterer SG, Bakula D, Cezanne A, Robenek H, Proikas-Cezanne T
Biochemical Society transactions42(5)1414-7(2014 Oct)

WIPI-dependent autophagy during neutrophil differentiation of NB4 acute promyelocytic leukemia cells.
Brigger D, Proikas-Cezanne T, Tschan MP
Cell death & disease5e1315(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WIPI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WIPI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu50513	NM_001320772.1 Latest version!	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu86962	XM_017024808.1 Latest version!	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu02640	NM_017983.7 Latest version!	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu02640	NM_017983.6	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WIPI1 ( NM_017983.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060453.3 Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02640
Clone ID Related Accession (Same CDS sequence)	NM_017983.6 , NM_017983.7
Accession Version	NM_017983.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	WD repeat domain phosphoinositide-interacting protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007780.10, BI764142.1 and BC039867.2. On Mar 5, 2016 this sequence version replaced NM_017983.5. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039867.2, SRR1803617.92240.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCG AGGCCGCGGA CGCTCCCCCG GGCGGGGTTG AGTCGGCGCT CAGCTGCTTC 
TCTTTCAACC AGGACTGCAC ATCCCTAGCA ACTGGAACTA AAGCCGGGTA TAAGCTGTTT 
TCTCTGAGTT CTGTGGAGCA GCTGGATCAA GTCCACGGAA GCAATGAAAT CCCGGACGTC 
TACATCGTGG AGCGCCTCTT CTCCAGCAGC CTGGTGGTGG TAGTCAGTCA CACAAAACCA 
CGGCAGATGA ACGTGTATCA CTTCAAGAAA GGCACAGAGA TCTGTAATTA CAGCTACTCC 
AGCAACATCT TGTCCATAAG GCTGAACCGG CAAAGGCTGC TGGTTTGCCT AGAAGAGTCC 
ATTTATATTC ACAACATTAA AGACATGAAG CTGTTGAAGA CCCTCCTGGA TATTCCTGCA 
AACCCAACAG GTCTATGTGC TCTCTCTATC AACCATTCCA ATTCTTACCT GGCCTATCCT 
GGAAGCCTGA CTTCAGGGGA GATTGTGCTT TATGATGGAA ACTCCCTGAA AACAGTCTGC 
ACTATTGCTG CCCATGAGGG AACACTAGCT GCCATCACCT TCAATGCCTC AGGCTCCAAA 
CTAGCAAGTG CGTCTGAAAA AGGCACAGTC ATCCGGGTGT TCTCTGTCCC TGATGGGCAA 
AAGCTCTATG AGTTCCGGAG AGGGATGAAA AGGTATGTGA CAATCAGCTC TCTAGTGTTC 
AGTATGGATT CACAATTCCT CTGCGCCTCC AGTAACACCG AGACGGTACA CATCTTCAAG 
CTGGAACAGG TCACCAACAG TCGACCAGAA GAGCCTTCGA CCTGGAGTGG CTACATGGGA 
AAGATGTTTA TGGCTGCTAC CAACTACCTC CCTACCCAGG TGTCAGACAT GATGCATCAG 
GACAGGGCTT TTGCCACTGC ACGCTTGAAC TTCTCCGGAC AGAGGAACAT CTGTACCCTC 
TCAACGATCC AGAAGTTGCC ACGGCTGCTA GTTGCGTCAT CCAGTGGACA CCTTTATATG 
TACAATTTGG ATCCTCAGGA TGGAGGAGAG TGTGTCTTAA TCAAAACCCA CAGCTTGCTT 
GGCTCAGGAA CAACAGAAGA GAATAAAGAA AATGACCTCA GACCTTCCTT ACCTCAGTCT 
TATGCAGCGA CCGTAGCCAG ACCAAGTGCA TCTTCAGCCT CCACGGTGCC AGGTTATTCT 
GAGGACGGCG GGGCGCTGCG AGGAGAAGTT ATTCCTGAAC ATGAGTTTGC GACGGGACCA 
GTGTGTCTTG ATGATGAGAA TGAGTTTCCT CCTATAATCT TGTGCCGTGG AAATCAGAAG 
GGCAAAACGA AGCAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060453.3
CDS	104..1444
Translation	MEAEAADAPPGGVESALSCFSFNQDCTSLATGTKAGYKLFSLSSVEQLDQVHGSNEIPDVYIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLLVCLEESIYIHNIKDMKLLKTLLDIPANPTGLCALSINHSNSYLAYPGSLTSGEIVLYDGNSLKTVCTIAAHEGTLAAITFNASGSKLASASEKGTVIRVFSVPDGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKLEQVTNSRPEEPSTWSGYMGKMFMAATNYLPTQVSDMMHQDRAFATARLNFSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGECVLIKTHSLLGSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPIILCRGNQKGKTKQS

Target ORF information:

RefSeq Version	NM_017983.6
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017983.6

ORF Insert Sequence:

ATGGAGGCCG AGGCCGCGGA CGCTCCCCCG GGCGGGGTTG AGTCGGCGCT CAGCTGCTTC 
TCTTTCAACC AGGACTGCAC ATCCCTAGCA ACTGGAACTA AAGCCGGGTA TAAGCTGTTT 
TCTCTGAGTT CTGTGGAGCA GCTGGATCAA GTCCACGGAA GCAATGAAAT CCCGGACGTC 
TACATCGTGG AGCGCCTCTT CTCCAGCAGC CTGGTGGTGG TAGTCAGTCA CACAAAACCA 
CGGCAGATGA ACGTGTATCA CTTCAAGAAA GGCACAGAGA TCTGTAATTA CAGCTACTCC 
AGCAACATCT TGTCCATAAG GCTGAACCGG CAAAGGCTGC TGGTTTGCCT AGAAGAGTCC 
ATTTATATTC ACAACATTAA AGACATGAAG CTGTTGAAGA CCCTCCTGGA TATTCCTGCA 
AACCCAACAG GTCTATGTGC TCTCTCTATC AACCATTCCA ATTCTTACCT GGCCTATCCT 
GGAAGCCTGA CTTCAGGGGA GATTGTGCTT TATGATGGAA ACTCCCTGAA AACAGTCTGC 
ACTATTGCTG CCCATGAGGG AACACTAGCT GCCATCACCT TCAATGCCTC AGGCTCCAAA 
CTAGCAAGTG CGTCTGAAAA AGGCACAGTC ATCCGGGTGT TCTCTGTCCC TGATGGGCAA 
AAGCTCTATG AGTTCCGGAG AGGGATGAAA AGGTATGTGA CAATCAGCTC TCTAGTGTTC 
AGTATGGATT CACAATTCCT CTGCGCCTCC AGTAACACCG AGACGGTACA CATCTTCAAG 
CTGGAACAGG TCACCAACAG TCGACCAGAA GAGCCTTCGA CCTGGAGTGG CTACATGGGA 
AAGATGTTTA TGGCTGCTAC CAACTACCTC CCTACCCAGG TGTCAGACAT GATGCATCAG 
GACAGGGCTT TTGCCACTGC ACGCTTGAAC TTCTCCGGAC AGAGGAACAT CTGTACCCTC 
TCAACGATCC AGAAGTTGCC ACGGCTGCTA GTTGCGTCAT CCAGTGGACA CCTTTATATG 
TACAATTTGG ATCCTCAGGA TGGAGGAGAG TGTGTCTTAA TCAAAACCCA CAGCTTGCTT 
GGCTCAGGAA CAACAGAAGA GAATAAAGAA AATGACCTCA GACCTTCCTT ACCTCAGTCT 
TATGCAGCGA CCGTAGCCAG ACCAAGTGCA TCTTCAGCCT CCACGGTGCC AGGTTATTCT 
GAGGACGGCG GGGCGCTGCG AGGAGAAGTT ATTCCTGAAC ATGAGTTTGC GACGGGACCA 
GTGTGTCTTG ATGATGAGAA TGAGTTTCCT CCTATAATCT TGTGCCGTGG AAATCAGAAG 
GGCAAAACGA AGCAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WIPI1 ( NM_001320772.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307701.1 Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu50513
Clone ID Related Accession (Same CDS sequence)	NM_001320772.1
Accession Version	NM_001320772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553356800000
Organism	Homo sapiens(human)
Product	WD repeat domain phosphoinositide-interacting protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC007780.10, BI764142.1, AK301830.1 and BC039867.2. On Mar 5, 2016 this sequence version replaced XM_005257497.3. Transcript Variant: This variant (2) lacks an alternate exon, resulting in a novel 5' UTR and the use of a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.49089.1, SRR1660803.58224.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACGTGT ATCACTTCAA GAAAGGCACA GAGATCTGTA ATTACAGCTA CTCCAGCAAC 
ATCTTGTCCA TAAGGCTGAA CCGGCAAAGG CTGCTGGTTT GCCTAGAAGA GTCCATTTAT 
ATTCACAACA TTAAAGACAT GAAGCTGTTG AAGACCCTCC TGGATATTCC TGCAAACCCA 
ACAGGTCTAT GTGCTCTCTC TATCAACCAT TCCAATTCTT ACCTGGCCTA TCCTGGAAGC 
CTGACTTCAG GGGAGATTGT GCTTTATGAT GGAAACTCCC TGAAAACAGT CTGCACTATT 
GCTGCCCATG AGGGAACACT AGCTGCCATC ACCTTCAATG CCTCAGGCTC CAAACTAGCA 
AGTGCGTCTG AAAAAGGCAC AGTCATCCGG GTGTTCTCTG TCCCTGATGG GCAAAAGCTC 
TATGAGTTCC GGAGAGGGAT GAAAAGGTAT GTGACAATCA GCTCTCTAGT GTTCAGTATG 
GATTCACAAT TCCTCTGCGC CTCCAGTAAC ACCGAGACGG TACACATCTT CAAGCTGGAA 
CAGGTCACCA ACAGTCGACC AGAAGAGCCT TCGACCTGGA GTGGCTACAT GGGAAAGATG 
TTTATGGCTG CTACCAACTA CCTCCCTACC CAGGTGTCAG ACATGATGCA TCAGGACAGG 
GCTTTTGCCA CTGCACGCTT GAACTTCTCC GGACAGAGGA ACATCTGTAC CCTCTCAACG 
ATCCAGAAGT TGCCACGGCT GCTAGTTGCG TCATCCAGTG GACACCTTTA TATGTACAAT 
TTGGATCCTC AGGATGGAGG AGAGTGTGTC TTAATCAAAA CCCACAGCTT GCTTGGCTCA 
GGAACAACAG AAGAGAATAA AGAAAATGAC CTCAGACCTT CCTTACCTCA GTCTTATGCA 
GCGACCGTAG CCAGACCAAG TGCATCTTCA GCCTCCACGG TGCCAGGTTA TTCTGAGGAC 
GGCGGGGCGC TGCGAGGAGA AGTTATTCCT GAACATGAGT TTGCGACGGG ACCAGTGTGT 
CTTGATGATG AGAATGAGTT TCCTCCTATA ATCTTGTGCC GTGGAAATCA GAAGGGCAAA 
ACGAAGCAGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307701.1
CDS	267..1361
Translation	MNVYHFKKGTEICNYSYSSNILSIRLNRQRLLVCLEESIYIHNIKDMKLLKTLLDIPANPTGLCALSINHSNSYLAYPGSLTSGEIVLYDGNSLKTVCTIAAHEGTLAAITFNASGSKLASASEKGTVIRVFSVPDGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKLEQVTNSRPEEPSTWSGYMGKMFMAATNYLPTQVSDMMHQDRAFATARLNFSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGECVLIKTHSLLGSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPIILCRGNQKGKTKQS

Target ORF information:

RefSeq Version	NM_001320772.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320772.1

ORF Insert Sequence:

ATGAACGTGT ATCACTTCAA GAAAGGCACA GAGATCTGTA ATTACAGCTA CTCCAGCAAC 
ATCTTGTCCA TAAGGCTGAA CCGGCAAAGG CTGCTGGTTT GCCTAGAAGA GTCCATTTAT 
ATTCACAACA TTAAAGACAT GAAGCTGTTG AAGACCCTCC TGGATATTCC TGCAAACCCA 
ACAGGTCTAT GTGCTCTCTC TATCAACCAT TCCAATTCTT ACCTGGCCTA TCCTGGAAGC 
CTGACTTCAG GGGAGATTGT GCTTTATGAT GGAAACTCCC TGAAAACAGT CTGCACTATT 
GCTGCCCATG AGGGAACACT AGCTGCCATC ACCTTCAATG CCTCAGGCTC CAAACTAGCA 
AGTGCGTCTG AAAAAGGCAC AGTCATCCGG GTGTTCTCTG TCCCTGATGG GCAAAAGCTC 
TATGAGTTCC GGAGAGGGAT GAAAAGGTAT GTGACAATCA GCTCTCTAGT GTTCAGTATG 
GATTCACAAT TCCTCTGCGC CTCCAGTAAC ACCGAGACGG TACACATCTT CAAGCTGGAA 
CAGGTCACCA ACAGTCGACC AGAAGAGCCT TCGACCTGGA GTGGCTACAT GGGAAAGATG 
TTTATGGCTG CTACCAACTA CCTCCCTACC CAGGTGTCAG ACATGATGCA TCAGGACAGG 
GCTTTTGCCA CTGCACGCTT GAACTTCTCC GGACAGAGGA ACATCTGTAC CCTCTCAACG 
ATCCAGAAGT TGCCACGGCT GCTAGTTGCG TCATCCAGTG GACACCTTTA TATGTACAAT 
TTGGATCCTC AGGATGGAGG AGAGTGTGTC TTAATCAAAA CCCACAGCTT GCTTGGCTCA 
GGAACAACAG AAGAGAATAA AGAAAATGAC CTCAGACCTT CCTTACCTCA GTCTTATGCA 
GCGACCGTAG CCAGACCAAG TGCATCTTCA GCCTCCACGG TGCCAGGTTA TTCTGAGGAC 
GGCGGGGCGC TGCGAGGAGA AGTTATTCCT GAACATGAGT TTGCGACGGG ACCAGTGTGT 
CTTGATGATG AGAATGAGTT TCCTCCTATA ATCTTGTGCC GTGGAAATCA GAAGGGCAAA 
ACGAAGCAGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WIPI1 ( XM_017024808.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880297.1 Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86962
Clone ID Related Accession (Same CDS sequence)	XM_017024808.1
Accession Version	XM_017024808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	WD repeat domain phosphoinositide-interacting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGT TGAAGACCCT CCTGGATATT CCTGCAAACC CAACAGGTCT ATGTGCTCTC 
TCTATCAACC ATTCCAATTC TTACCTGGCC TATCCTGGAA GCCTGACTTC AGGGGAGATT 
GTGCTTTATG ATGGAAACTC CCTGAAAACA GTCTGCACTA TTGCTGCCCA TGAGGGAACA 
CTAGCTGCCA TCACCTTCAA TGCCTCAGGC TCCAAACTAG CAAGTGCGTC TGAAAAAGGC 
ACAGTCATCC GGGTGTTCTC TGTCCCTGAT GGGCAAAAGC TCTATGAGTT CCGGAGAGGG 
ATGAAAAGGT ATGTGACAAT CAGCTCTCTA GTGTTCAGTA TGGATTCACA ATTCCTCTGC 
GCCTCCAGTA ACACCGAGAC GGTACACATC TTCAAGCTGG AACAGGTCAC CAACAGTCGA 
CCAGAAGAGC CTTCGACCTG GAGTGGCTAC ATGGGAAAGA TGTTTATGGC TGCTACCAAC 
TACCTCCCTA CCCAGGTGTC AGACATGATG CATCAGGACA GGGCTTTTGC CACTGCACGC 
TTGAACTTCT CCGGACAGAG GAACATCTGT ACCCTCTCAA CGATCCAGAA GTTGCCACGG 
CTGCTAGTTG CGTCATCCAG TGGACACCTT TATATGTACA ATTTGGATCC TCAGGATGGA 
GGAGAGTGTG TCTTAATCAA AACCCACAGC TTGCTTGGCT CAGGAACAAC AGAAGAGAAT 
AAAGAAAATG ACCTCAGACC TTCCTTACCT CAGTCTTATG CAGCGACCGT AGCCAGACCA 
AGTGCATCTT CAGCCTCCAC GGTGCCAGGT TATTCTGAGG ACGGCGGGGC GCTGCGAGGA 
GAAGTTATTC CTGAACATGA GTTTGCGACG GGACCAGTGT GTCTTGATGA TGAGAATGAG 
TTTCCTCCTA TAATCTTGTG CCGTGGAAAT CAGAAGGGCA AAACGAAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880297.1
CDS	2442..3398
Translation	MKLLKTLLDIPANPTGLCALSINHSNSYLAYPGSLTSGEIVLYDGNSLKTVCTIAAHEGTLAAITFNASGSKLASASEKGTVIRVFSVPDGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKLEQVTNSRPEEPSTWSGYMGKMFMAATNYLPTQVSDMMHQDRAFATARLNFSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGECVLIKTHSLLGSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPIILCRGNQKGKTKQS

Target ORF information:

RefSeq Version	XM_017024808.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024808.1

ORF Insert Sequence:

ATGAAGCTGT TGAAGACCCT CCTGGATATT CCTGCAAACC CAACAGGTCT ATGTGCTCTC 
TCTATCAACC ATTCCAATTC TTACCTGGCC TATCCTGGAA GCCTGACTTC AGGGGAGATT 
GTGCTTTATG ATGGAAACTC CCTGAAAACA GTCTGCACTA TTGCTGCCCA TGAGGGAACA 
CTAGCTGCCA TCACCTTCAA TGCCTCAGGC TCCAAACTAG CAAGTGCGTC TGAAAAAGGC 
ACAGTCATCC GGGTGTTCTC TGTCCCTGAT GGGCAAAAGC TCTATGAGTT CCGGAGAGGG 
ATGAAAAGGT ATGTGACAAT CAGCTCTCTA GTGTTCAGTA TGGATTCACA ATTCCTCTGC 
GCCTCCAGTA ACACCGAGAC GGTACACATC TTCAAGCTGG AACAGGTCAC CAACAGTCGA 
CCAGAAGAGC CTTCGACCTG GAGTGGCTAC ATGGGAAAGA TGTTTATGGC TGCTACCAAC 
TACCTCCCTA CCCAGGTGTC AGACATGATG CATCAGGACA GGGCTTTTGC CACTGCACGC 
TTGAACTTCT CCGGACAGAG GAACATCTGT ACCCTCTCAA CGATCCAGAA GTTGCCACGG 
CTGCTAGTTG CGTCATCCAG TGGACACCTT TATATGTACA ATTTGGATCC TCAGGATGGA 
GGAGAGTGTG TCTTAATCAA AACCCACAGC TTGCTTGGCT CAGGAACAAC AGAAGAGAAT 
AAAGAAAATG ACCTCAGACC TTCCTTACCT CAGTCTTATG CAGCGACCGT AGCCAGACCA 
AGTGCATCTT CAGCCTCCAC GGTGCCAGGT TATTCTGAGG ACGGCGGGGC GCTGCGAGGA 
GAAGTTATTC CTGAACATGA GTTTGCGACG GGACCAGTGT GTCTTGATGA TGAGAATGAG 
TTTCCTCCTA TAATCTTGTG CCGTGGAAAT CAGAAGGGCA AAACGAAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WIPI1 ( NM_017983.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_060453.3 Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02640
Clone ID Related Accession (Same CDS sequence)	NM_017983.6 , NM_017983.7
Accession Version	NM_017983.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556640000000
Organism	Homo sapiens(human)
Product	WD repeat domain phosphoinositide-interacting protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI764142.1, BC039867.2 and AC007780.10. On Nov 22, 2018 this sequence version replaced NM_017983.6. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.790677.1, SRR1803617.142671.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262139.10/ ENSP00000262139.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCCG AGGCCGCGGA CGCTCCCCCG GGCGGGGTTG AGTCGGCGCT CAGCTGCTTC 
TCTTTCAACC AGGACTGCAC ATCCCTAGCA ACTGGAACTA AAGCCGGGTA TAAGCTGTTT 
TCTCTGAGTT CTGTGGAGCA GCTGGATCAA GTCCACGGAA GCAATGAAAT CCCGGACGTC 
TACATCGTGG AGCGCCTCTT CTCCAGCAGC CTGGTGGTGG TAGTCAGTCA CACAAAACCA 
CGGCAGATGA ACGTGTATCA CTTCAAGAAA GGCACAGAGA TCTGTAATTA CAGCTACTCC 
AGCAACATCT TGTCCATAAG GCTGAACCGG CAAAGGCTGC TGGTTTGCCT AGAAGAGTCC 
ATTTATATTC ACAACATTAA AGACATGAAG CTGTTGAAGA CCCTCCTGGA TATTCCTGCA 
AACCCAACAG GTCTATGTGC TCTCTCTATC AACCATTCCA ATTCTTACCT GGCCTATCCT 
GGAAGCCTGA CTTCAGGGGA GATTGTGCTT TATGATGGAA ACTCCCTGAA AACAGTCTGC 
ACTATTGCTG CCCATGAGGG AACACTAGCT GCCATCACCT TCAATGCCTC AGGCTCCAAA 
CTAGCAAGTG CGTCTGAAAA AGGCACAGTC ATCCGGGTGT TCTCTGTCCC TGATGGGCAA 
AAGCTCTATG AGTTCCGGAG AGGGATGAAA AGGTATGTGA CAATCAGCTC TCTAGTGTTC 
AGTATGGATT CACAATTCCT CTGCGCCTCC AGTAACACCG AGACGGTACA CATCTTCAAG 
CTGGAACAGG TCACCAACAG TCGACCAGAA GAGCCTTCGA CCTGGAGTGG CTACATGGGA 
AAGATGTTTA TGGCTGCTAC CAACTACCTC CCTACCCAGG TGTCAGACAT GATGCATCAG 
GACAGGGCTT TTGCCACTGC ACGCTTGAAC TTCTCCGGAC AGAGGAACAT CTGTACCCTC 
TCAACGATCC AGAAGTTGCC ACGGCTGCTA GTTGCGTCAT CCAGTGGACA CCTTTATATG 
TACAATTTGG ATCCTCAGGA TGGAGGAGAG TGTGTCTTAA TCAAAACCCA CAGCTTGCTT 
GGCTCAGGAA CAACAGAAGA GAATAAAGAA AATGACCTCA GACCTTCCTT ACCTCAGTCT 
TATGCAGCGA CCGTAGCCAG ACCAAGTGCA TCTTCAGCCT CCACGGTGCC AGGTTATTCT 
GAGGACGGCG GGGCGCTGCG AGGAGAAGTT ATTCCTGAAC ATGAGTTTGC GACGGGACCA 
GTGTGTCTTG ATGATGAGAA TGAGTTTCCT CCTATAATCT TGTGCCGTGG AAATCAGAAG 
GGCAAAACGA AGCAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060453.3
CDS	76..1416
Translation	MEAEAADAPPGGVESALSCFSFNQDCTSLATGTKAGYKLFSLSSVEQLDQVHGSNEIPDVYIVERLFSSSLVVVVSHTKPRQMNVYHFKKGTEICNYSYSSNILSIRLNRQRLLVCLEESIYIHNIKDMKLLKTLLDIPANPTGLCALSINHSNSYLAYPGSLTSGEIVLYDGNSLKTVCTIAAHEGTLAAITFNASGSKLASASEKGTVIRVFSVPDGQKLYEFRRGMKRYVTISSLVFSMDSQFLCASSNTETVHIFKLEQVTNSRPEEPSTWSGYMGKMFMAATNYLPTQVSDMMHQDRAFATARLNFSGQRNICTLSTIQKLPRLLVASSSGHLYMYNLDPQDGGECVLIKTHSLLGSGTTEENKENDLRPSLPQSYAATVARPSASSASTVPGYSEDGGALRGEVIPEHEFATGPVCLDDENEFPPIILCRGNQKGKTKQS

Target ORF information:

RefSeq Version	NM_017983.7
Organism	Homo sapiens(human)
Definition	Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017983.7

ORF Insert Sequence:

ATGGAGGCCG AGGCCGCGGA CGCTCCCCCG GGCGGGGTTG AGTCGGCGCT CAGCTGCTTC 
TCTTTCAACC AGGACTGCAC ATCCCTAGCA ACTGGAACTA AAGCCGGGTA TAAGCTGTTT 
TCTCTGAGTT CTGTGGAGCA GCTGGATCAA GTCCACGGAA GCAATGAAAT CCCGGACGTC 
TACATCGTGG AGCGCCTCTT CTCCAGCAGC CTGGTGGTGG TAGTCAGTCA CACAAAACCA 
CGGCAGATGA ACGTGTATCA CTTCAAGAAA GGCACAGAGA TCTGTAATTA CAGCTACTCC 
AGCAACATCT TGTCCATAAG GCTGAACCGG CAAAGGCTGC TGGTTTGCCT AGAAGAGTCC 
ATTTATATTC ACAACATTAA AGACATGAAG CTGTTGAAGA CCCTCCTGGA TATTCCTGCA 
AACCCAACAG GTCTATGTGC TCTCTCTATC AACCATTCCA ATTCTTACCT GGCCTATCCT 
GGAAGCCTGA CTTCAGGGGA GATTGTGCTT TATGATGGAA ACTCCCTGAA AACAGTCTGC 
ACTATTGCTG CCCATGAGGG AACACTAGCT GCCATCACCT TCAATGCCTC AGGCTCCAAA 
CTAGCAAGTG CGTCTGAAAA AGGCACAGTC ATCCGGGTGT TCTCTGTCCC TGATGGGCAA 
AAGCTCTATG AGTTCCGGAG AGGGATGAAA AGGTATGTGA CAATCAGCTC TCTAGTGTTC 
AGTATGGATT CACAATTCCT CTGCGCCTCC AGTAACACCG AGACGGTACA CATCTTCAAG 
CTGGAACAGG TCACCAACAG TCGACCAGAA GAGCCTTCGA CCTGGAGTGG CTACATGGGA 
AAGATGTTTA TGGCTGCTAC CAACTACCTC CCTACCCAGG TGTCAGACAT GATGCATCAG 
GACAGGGCTT TTGCCACTGC ACGCTTGAAC TTCTCCGGAC AGAGGAACAT CTGTACCCTC 
TCAACGATCC AGAAGTTGCC ACGGCTGCTA GTTGCGTCAT CCAGTGGACA CCTTTATATG 
TACAATTTGG ATCCTCAGGA TGGAGGAGAG TGTGTCTTAA TCAAAACCCA CAGCTTGCTT 
GGCTCAGGAA CAACAGAAGA GAATAAAGAA AATGACCTCA GACCTTCCTT ACCTCAGTCT 
TATGCAGCGA CCGTAGCCAG ACCAAGTGCA TCTTCAGCCT CCACGGTGCC AGGTTATTCT 
GAGGACGGCG GGGCGCTGCG AGGAGAAGTT ATTCCTGAAC ATGAGTTTGC GACGGGACCA 
GTGTGTCTTG ATGATGAGAA TGAGTTTCCT CCTATAATCT TGTGCCGTGG AAATCAGAAG 
GGCAAAACGA AGCAGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WIPI1, BAG1, and PEX3 Autophagy-Related Genes Are Relevant Melanoma Markers.
Oxidative medicine and cellular longevity20181471682(2018)
D'Arcangelo D,Giampietri C,Muscio M,Scatozza F,Facchiano F,Facchiano A

WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.
Nature communications815637(2017 05)
Bakula D,Müller AJ,Zuleger T,Takacs Z,Franz-Wachtel M,Thost AK,Brigger D,Tschan MP,Frickey T,Robenek H,Macek B,Proikas-Cezanne T

Fluorescence-based imaging of autophagy progression by human WIPI protein detection.
Methods (San Diego, Calif.)7569-78(2015 Mar)
Thost AK,Dönnes P,Kohlbacher O,Proikas-Cezanne T

WIPI β-propellers at the crossroads of autophagosome and lipid droplet dynamics.
Biochemical Society transactions42(5)1414-7(2014 Oct)
Pfisterer SG,Bakula D,Cezanne A,Robenek H,Proikas-Cezanne T

WIPI-dependent autophagy during neutrophil differentiation of NB4 acute promyelocytic leukemia cells.
Cell death & disease5e1315(2014 Jul)
Brigger D,Proikas-Cezanne T,Tschan MP

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WIPI1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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