PPP1R3A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R3A
Entrez Gene ID	5506
Full Name	protein phosphatase 1 regulatory subunit 3A
Synonyms	GM,PP1G,PPP1R3
General protein information	Preferred Names protein phosphatase 1 regulatory subunit 3A Names protein phosphatase 1 regulatory subunit 3A RG1 glycogen and sarcoplasmic reticulum binding subunit, skeletal muscle glycogen-associated regulatory subunit of protein phosphatase-1 protein phosphatase 1 glycogen- associated regulatory subunit protein phosphatase 1 regulatory subunit GM protein phosphatase 1, regulatory (inhibitor) subunit 3A protein phosphatase type-1 glycogen targeting subunit serine /threonine specific protein phosphatase type-1 protein phosphatase skeletal muscle glycogen targeting subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q31.1
Summary	The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq, Jul 2008].
Disorder MIM:	600917
Disorder Html:	Insulin resistance, severe, digenic, 125853 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002711.3	NP_002702.2	protein phosphatase 1 regulatory subunit 3A
XM_005250473.3	XP_005250530.1	protein phosphatase 1 regulatory subunit 3A isoform X1
NM_002711.4	NP_002702.2	protein phosphatase 1 regulatory subunit 3A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1R3A	NP_002702.2
Canis lupus familiaris (dog)	PPP1R3A	XP_539528.2
Pan troglodytes (chimpanzee)	PPP1R3A	XP_519320.3
Macaca mulatta (Rhesus monkey)	PPP1R3A	XP_001100958.2
Bos taurus (cattle)	PPP1R3A	NP_001192369.1
Mus musculus (house mouse)	Ppp1r3a	NP_536712.2
Rattus norvegicus (Norway rat)	Ppp1r3a	NP_001102692.1
Gallus gallus (chicken)	PPP1R3A	XP_004937598.1

Gene Ontology
Bibliography

Related articles in PubMed

Pathologic gene network rewiring implicates PPP1R3A as a central regulator in pressure overload heart failure.
Cordero P, Parikh VN, Chin ET, Erbilgin A, Gloudemans MJ, Shang C, Huang Y, Chang AC, Smith KS, Dewey F, Zaleta K, Morley M, Brandimarto J, Glazer N, Waggott D, Pavlovic A, Zhao M, Moravec CS, Tang WHW, Skreen J, Malloy C, Hannenhalli S, Li H, Ritter S, Li M, Bernstein D, Connolly A, Hakonarson H, Lusis AJ, Margulies KB, Depaoli-Roach AA, Montgomery SB, Wheeler MT, Cappola T, Ashley EA
Nature communications10(1)2760(2019 06)

Whole-exome sequencing in maya indigenous families: variant in PPP1R3A is associated with type 2 diabetes.
Sánchez-Pozos K, Ortíz-López MG, Peña-Espinoza BI, de Los Ángeles Granados-Silvestre M, Jiménez-Jacinto V, Verleyen J, Tekola-Ayele F, Sanchez-Flores A, Menjivar M
Molecular genetics and genomics : MGG293(5)1205-1216(2018 Oct)

Molecular Differences between Screen-Detected and Interval Breast Cancers Are Largely Explained by PAM50 Subtypes.
Li J, Ivansson E, Klevebring D, Tobin NP, Lindström LS, Holm J, Prochazka G, Cristando C, Palmgren J, Törnberg S, Humphreys K, Hartman J, Frisell J, Rantalainen M, Lindberg J, Hall P, Bergh J, Grönberg H, Czene K
Clinical cancer research : an official journal of the American Association for Cancer Research23(10)2584-2592(2017 May)

Gene-smoking interactions identify several novel blood pressure loci in the Framingham Heart Study.
Sung YJ, de Las Fuentes L, Schwander KL, Simino J, Rao DC
American journal of hypertension28(3)343-54(2015 Mar)

A novel interaction between hScrib and PP1γ downregulates ERK signaling and suppresses oncogene-induced cell transformation.
Nagasaka K, Seiki T, Yamashita A, Massimi P, Subbaiah VK, Thomas M, Kranjec C, Kawana K, Nakagawa S, Yano T, Taketani Y, Fujii T, Kozuma S, Banks L
PloS one8(1)e53752(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPP1R3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu41636	XM_005250473.3 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu17972	NM_002711.4 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
View Old Accession Versions >>
OHu17972	NM_002711.3	Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R3A ( XM_005250473.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005250530.1 Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), transcript variant X1, mRNA.

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CloneID	OHu41636
Clone ID Related Accession (Same CDS sequence)	XM_005250473.3
Accession Version	XM_005250473.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005250473.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TACTTTCCAA CCTGGTTTCT CCCCTCAACC AAGTAGACGA GGTTCTGATT 
CTTCTGAAGA CATATACCTG GATACCCCAT CTTCAGAAAG AACAAGAGCC GGAGCCTGTA 
AAACCATGGA AAGAAGTTCC TAACAGACAA ATAAAAGGCT GCTTAAAGGT AAAATCAAGT 
AAAGAAGAAT CATCAGTAAC ATCAGAAGAA AATAACTTTG AGAATCCAAA GAATACAGAT 
ACCTATATCC CAACAATCAT TTGTTCTCAT GAGGACAAGG AAGATTTGGA AGCCAGTAAT 
CGAAATGTAA AAGATGTAAA CAGGGAACAT GATGAACATA ATGAAAAAGA ATTAGAGTTG 
ATGATAAATC AACACTTAAT AAGAACCAGA AGTACTGCTT CCAGAGATGA AAGGAATACA 
TTTTCAACAG ATCCAGTCAA TTTTCCAAAT AAAGCAGAGG GGTTAGAGAA GAAGCAAATC 
CATGGTGAAA TATGTACTGA CTTGTTCCAA AGGTCTCTGT CTCCAAGTTC ATCAGCAGAA 
AGCTCCGTAA AGGGAGATTT TTACTGCAAT GAAAAATATT CCTCAGGAGA TGACTGTACA 
CATCAACCTT CAGAGGAAAC TACTTCAAAT ATGGGAGAAA TCAAGCCATC ATTGGGAGAT 
ACTAGTAGTG ATGAACTAGT GCAATTACAT ACTGGCAGCA AAGAAGTCCT GGATGATAAT 
GCTAATCCAG CCCATGGCAA TGGCACAGTG CAAATACCTT GCCCCTCTTC AGATCAACTA 
ATGGCAGGAA ACCTTAATAA AAAACATGAA GGAGGAGCTA AAAATATTGA AGTAAAAGAT 
TTGGGATGTT TACGAAGAGA TTTCCATTCA GATACGTCGG CATGTCTCAA AGAATCAACA 
GAAGAAGGAT CTTCTAAGGA AGATTATTAT GGCAATGGTA AGGATGATGA AGAACAAAGA 
ATATATTTAG GTGTTAATGA AAAACAAAGA AAAAATTTCC AAACAATCTT ACATGACCAA 
GAAAGGAAGA TGGGTAACCC TAAAATAAGT GTGGCAGGGA TTGGAGCTAG TAACAGAGAC 
CTGGCTACTC TGCTGAGCGA ACATACCGCA ATCCCCACCC GGGCAATCAC AGCAGATGTG 
TCTCATTCAC CAAGGACAAA TTTAAGTTGG GAAGAAGCTG TGTTAACCCC AGAGCATCAT 
CATTTGACTA GTGAAGGCAG CGCTTTAGGA GGGATAACTG GTCAAGTTTG TTCATCAAGA 
ACTGGAAATG TTTTGAGGAA TGATTATCTT TTCCAAGTTG AAGAAAAATC AGGTGGGATT 
AATTCTGAAG ATCAGGATAA TAGCCCACAG CATAAACAAA GTTGGAATGT TCTGGAAAGT 
CAGGGAAAAT CAAGAGAGAA TAAGACAAAC ATAACAGAGC ATATCAAAGG ACAAACAGAT 
TGTGAAGACG TGTGGGGAAA AAGAGATAAT ACGAGGAGTT TGAAAGCTAC TACAGAAGAA 
TTGTTTACCT GCCAAGAAAC AGTGTGCTGT GAACTGTCTT CTCTAGCTGA TCATGGCATT 
ACTGAGAAAG CAGAAGCTGG TACAGCCTAT ATAATTAAGA CAACATCAGA AAGTACTCCA 
GAAAGCATGT CTGCTAGAGA AAAAGCAATA ATTGCTAAGC TACCTCAAGA GACAGCACGA 
AGTGACAGGC CCATCGAGGT AAAGGAAACA GCGTTTGATC CACATGAAGG GAGAAATGAT 
GATTCACATT ATACCCTTTG TCAACGAGAT ACAGTAGGTG TAATCTATGA CAATGATTTT 
GAAAAGGAAT CACGTTTAGG TATTTGTAAT GTACGTGTAG ATGAAATGGA GAAGGAAGAA 
ACCATGTCTA TGTACAATCC TAGGAAGACA CATGACAGGG AGAAATGTGG CACTGGAAAT 
ATAACATCTG TGGAAGAATC CTCATGGGTC ATTACAGAAT ATCAAAAAGC AACTTCAAAA 
CTGGATTTAC AGTTGGGAAT GTTACCAACA GACAAAACTG TATTTTCAGA AAACAGAGAT 
CTTAGGCAGG TTCAAGAATT ATCAAAGAAA ACAGACTCGG ATGCCATTGT GCATTCTGCT 
TTTAACTCAG ACACTAATAG AGCTCCTCAG AATAGCTCTC CTTTTTCCAA ACATCATACT 
GAAATTTCAG TGTCAACTAA TGAGCAGGCA ATTGCTGTAG AGAATGCAGT TACTACCATG 
GCTAGCCAAC CTATTTCTAC GAAATCAGAA AATATTTGTA ATTCAACAAG AGAAATCCAG 
GGTATTGAGA AGCACCCTTA TCCTGAGTCT AAACCTGAAG AAGTTTCCAG AAGTTCAGGA 
ATAGTGACAT CAGGTAGTAG AAAAGAAAGA TGCATAGGCC AGATTTTCCA AACAGAAGAG 
TATAGTGTGG AAAAATCTCT AGGGCCAATG ATTTTAATCA ACAAACCTCT TGAGAATATG 
GAAGAAGCAA GGCATGAAAA TGAAGGATTA GTAAGCTCTG GGCAATCACT ATACACTTCA 
GGTGAAAAGG AATCTGACAG CTCTGCTTCT ACTAGTCTTC CTGTTGAGGA AAGTCAAGCT 
CAAGGCAACG AATCTTTGTT TTCAAAATAT ACCAACTCTA AAATACCTTA TTTCCTTTTG 
TTTCTGATAT TTCTTATAAC TGTCTACCAT TATGACTTAA TGATTGGCTT GACATTCTAC 
GTTTTGTCAT TGTCCTGGCT ATCCTGGGAA GAGGGTAGAC AAAAAGAGTC TGTCAAAAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005250530.1
CDS	181..2946
Translation	MKKLLSNLVSPLNQVDEVLILLKTYTWIPHLQKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNTDTYIPTIICSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFPNKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLGDTSSDELVQLHTGSKEVLDDNANPAHGNGTVQIPCPSSDQLMAGNLNKKHEGGAKNIEVKDLGCLRRDFHSDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNRDLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDYLFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTEELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKETAFDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDLRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

Target ORF information:

RefSeq Version	XM_005250473.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005250473.3

ORF Insert Sequence:

ATGAAGAAGT TACTTTCCAA CCTGGTTTCT CCCCTCAACC AAGTAGACGA GGTTCTGATT 
CTTCTGAAGA CATATACCTG GATACCCCAT CTTCAGAAAG AACAAGAGCC GGAGCCTGTA 
AAACCATGGA AAGAAGTTCC TAACAGACAA ATAAAAGGCT GCTTAAAGGT AAAATCAAGT 
AAAGAAGAAT CATCAGTAAC ATCAGAAGAA AATAACTTTG AGAATCCAAA GAATACAGAT 
ACCTATATCC CAACAATCAT TTGTTCTCAT GAGGACAAGG AAGATTTGGA AGCCAGTAAT 
CGAAATGTAA AAGATGTAAA CAGGGAACAT GATGAACATA ATGAAAAAGA ATTAGAGTTG 
ATGATAAATC AACACTTAAT AAGAACCAGA AGTACTGCTT CCAGAGATGA AAGGAATACA 
TTTTCAACAG ATCCAGTCAA TTTTCCAAAT AAAGCAGAGG GGTTAGAGAA GAAGCAAATC 
CATGGTGAAA TATGTACTGA CTTGTTCCAA AGGTCTCTGT CTCCAAGTTC ATCAGCAGAA 
AGCTCCGTAA AGGGAGATTT TTACTGCAAT GAAAAATATT CCTCAGGAGA TGACTGTACA 
CATCAACCTT CAGAGGAAAC TACTTCAAAT ATGGGAGAAA TCAAGCCATC ATTGGGAGAT 
ACTAGTAGTG ATGAACTAGT GCAATTACAT ACTGGCAGCA AAGAAGTCCT GGATGATAAT 
GCTAATCCAG CCCATGGCAA TGGCACAGTG CAAATACCTT GCCCCTCTTC AGATCAACTA 
ATGGCAGGAA ACCTTAATAA AAAACATGAA GGAGGAGCTA AAAATATTGA AGTAAAAGAT 
TTGGGATGTT TACGAAGAGA TTTCCATTCA GATACGTCGG CATGTCTCAA AGAATCAACA 
GAAGAAGGAT CTTCTAAGGA AGATTATTAT GGCAATGGTA AGGATGATGA AGAACAAAGA 
ATATATTTAG GTGTTAATGA AAAACAAAGA AAAAATTTCC AAACAATCTT ACATGACCAA 
GAAAGGAAGA TGGGTAACCC TAAAATAAGT GTGGCAGGGA TTGGAGCTAG TAACAGAGAC 
CTGGCTACTC TGCTGAGCGA ACATACCGCA ATCCCCACCC GGGCAATCAC AGCAGATGTG 
TCTCATTCAC CAAGGACAAA TTTAAGTTGG GAAGAAGCTG TGTTAACCCC AGAGCATCAT 
CATTTGACTA GTGAAGGCAG CGCTTTAGGA GGGATAACTG GTCAAGTTTG TTCATCAAGA 
ACTGGAAATG TTTTGAGGAA TGATTATCTT TTCCAAGTTG AAGAAAAATC AGGTGGGATT 
AATTCTGAAG ATCAGGATAA TAGCCCACAG CATAAACAAA GTTGGAATGT TCTGGAAAGT 
CAGGGAAAAT CAAGAGAGAA TAAGACAAAC ATAACAGAGC ATATCAAAGG ACAAACAGAT 
TGTGAAGACG TGTGGGGAAA AAGAGATAAT ACGAGGAGTT TGAAAGCTAC TACAGAAGAA 
TTGTTTACCT GCCAAGAAAC AGTGTGCTGT GAACTGTCTT CTCTAGCTGA TCATGGCATT 
ACTGAGAAAG CAGAAGCTGG TACAGCCTAT ATAATTAAGA CAACATCAGA AAGTACTCCA 
GAAAGCATGT CTGCTAGAGA AAAAGCAATA ATTGCTAAGC TACCTCAAGA GACAGCACGA 
AGTGACAGGC CCATCGAGGT AAAGGAAACA GCGTTTGATC CACATGAAGG GAGAAATGAT 
GATTCACATT ATACCCTTTG TCAACGAGAT ACAGTAGGTG TAATCTATGA CAATGATTTT 
GAAAAGGAAT CACGTTTAGG TATTTGTAAT GTACGTGTAG ATGAAATGGA GAAGGAAGAA 
ACCATGTCTA TGTACAATCC TAGGAAGACA CATGACAGGG AGAAATGTGG CACTGGAAAT 
ATAACATCTG TGGAAGAATC CTCATGGGTC ATTACAGAAT ATCAAAAAGC AACTTCAAAA 
CTGGATTTAC AGTTGGGAAT GTTACCAACA GACAAAACTG TATTTTCAGA AAACAGAGAT 
CTTAGGCAGG TTCAAGAATT ATCAAAGAAA ACAGACTCGG ATGCCATTGT GCATTCTGCT 
TTTAACTCAG ACACTAATAG AGCTCCTCAG AATAGCTCTC CTTTTTCCAA ACATCATACT 
GAAATTTCAG TGTCAACTAA TGAGCAGGCA ATTGCTGTAG AGAATGCAGT TACTACCATG 
GCTAGCCAAC CTATTTCTAC GAAATCAGAA AATATTTGTA ATTCAACAAG AGAAATCCAG 
GGTATTGAGA AGCACCCTTA TCCTGAGTCT AAACCTGAAG AAGTTTCCAG AAGTTCAGGA 
ATAGTGACAT CAGGTAGTAG AAAAGAAAGA TGCATAGGCC AGATTTTCCA AACAGAAGAG 
TATAGTGTGG AAAAATCTCT AGGGCCAATG ATTTTAATCA ACAAACCTCT TGAGAATATG 
GAAGAAGCAA GGCATGAAAA TGAAGGATTA GTAAGCTCTG GGCAATCACT ATACACTTCA 
GGTGAAAAGG AATCTGACAG CTCTGCTTCT ACTAGTCTTC CTGTTGAGGA AAGTCAAGCT 
CAAGGCAACG AATCTTTGTT TTCAAAATAT ACCAACTCTA AAATACCTTA TTTCCTTTTG 
TTTCTGATAT TTCTTATAAC TGTCTACCAT TATGACTTAA TGATTGGCTT GACATTCTAC 
GTTTTGTCAT TGTCCTGGCT ATCCTGGGAA GAGGGTAGAC AAAAAGAGTC TGTCAAAAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R3A ( NM_002711.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002702.2 Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), mRNA.

Price & Availability↑

CloneID	OHu17972
Clone ID Related Accession (Same CDS sequence)	NM_002711.3 , NM_002711.4
Accession Version	NM_002711.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550505600000
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 3A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC126451.1, AC092465.5, X78578.1 and DB527300.2. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2008 this sequence version replaced NM_002711.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X78578.1, BC126451.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2149178 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCTT CTGAAGTACC TAGTCAGATT AGCAAAGATA ATTTTTTAGA AGTTCCTAAT 
TTATCTGACT CTCTTTGTGA AGATGAAGAA GTTACTTTCC AACCTGGTTT CTCCCCTCAA 
CCAAGTAGAC GAGGTTCTGA TTCTTCTGAA GACATATACC TGGATACCCC ATCTTCAGGT 
ACTAGAAGAG TTTCATTTGC TGATTCCTTT GGATTCAATC TTGTGTCTGT TAAAGAATTT 
GATTGCTGGG AATTACCGAG TGCTTCAACC ACTTTTGACT TAGGGACGGA CATTTTCCAC 
ACAGAAGAAT ATGTTTTAGC CCCACTGTTT GACTTGCCTT CTTCAAAAGA AGATCTTATG 
CAACAACTCC AAATACAGAA AGCAATACTG GAGTCAACTG AGTCTCTTCT TGGGTCTACA 
AGTATCAAGG GTATTATTCG AGTTTTGAAT GTTTCTTTTG AGAAGTTAGT ATATGTAAGA 
ATGTCTTTAG ATGACTGGCA GACACATTAT GACATTTTAG CAGAATATGT TCCTAATTCA 
TGTGATGGTG AAACTGACCA GTTCTCCTTT AAGATTGTAT TGGTTCCTCC TTATCAAAAA 
GATGGCAGTA AAGTTGAGTT TTGTATACGT TATGAAACTT CTGTTGGTAC ATTTTGGTCA 
AATAATAATG GCACAAATTA TACATTCATT TGTCAAAAGA AAGAACAAGA GCCGGAGCCT 
GTAAAACCAT GGAAAGAAGT TCCTAACAGA CAAATAAAAG GCTGCTTAAA GGTAAAATCA 
AGTAAAGAAG AATCATCAGT AACATCAGAA GAAAATAACT TTGAGAATCC AAAGAATACA 
GATACCTATA TCCCAACAAT CATTTGTTCT CATGAGGACA AGGAAGATTT GGAAGCCAGT 
AATCGAAATG TAAAAGATGT AAACAGGGAA CATGATGAAC ATAATGAAAA AGAATTAGAG 
TTGATGATAA ATCAACACTT AATAAGAACC AGAAGTACTG CTTCCAGAGA TGAAAGGAAT 
ACATTTTCAA CAGATCCAGT CAATTTTCCA AATAAAGCAG AGGGGTTAGA GAAGAAGCAA 
ATCCATGGTG AAATATGTAC TGACTTGTTC CAAAGGTCTC TGTCTCCAAG TTCATCAGCA 
GAAAGCTCCG TAAAGGGAGA TTTTTACTGC AATGAAAAAT ATTCCTCAGG AGATGACTGT 
ACACATCAAC CTTCAGAGGA AACTACTTCA AATATGGGAG AAATCAAGCC ATCATTGGGA 
GATACTAGTA GTGATGAACT AGTGCAATTA CATACTGGCA GCAAAGAAGT CCTGGATGAT 
AATGCTAATC CAGCCCATGG CAATGGCACA GTGCAAATAC CTTGCCCCTC TTCAGATCAA 
CTAATGGCAG GAAACCTTAA TAAAAAACAT GAAGGAGGAG CTAAAAATAT TGAAGTAAAA 
GATTTGGGAT GTTTACGAAG AGATTTCCAT TCAGATACGT CGGCATGTCT CAAAGAATCA 
ACAGAAGAAG GATCTTCTAA GGAAGATTAT TATGGCAATG GTAAGGATGA TGAAGAACAA 
AGAATATATT TAGGTGTTAA TGAAAAACAA AGAAAAAATT TCCAAACAAT CTTACATGAC 
CAAGAAAGGA AGATGGGTAA CCCTAAAATA AGTGTGGCAG GGATTGGAGC TAGTAACAGA 
GACCTGGCTA CTCTGCTGAG CGAACATACC GCAATCCCCA CCCGGGCAAT CACAGCAGAT 
GTGTCTCATT CACCAAGGAC AAATTTAAGT TGGGAAGAAG CTGTGTTAAC CCCAGAGCAT 
CATCATTTGA CTAGTGAAGG CAGCGCTTTA GGAGGGATAA CTGGTCAAGT TTGTTCATCA 
AGAACTGGAA ATGTTTTGAG GAATGATTAT CTTTTCCAAG TTGAAGAAAA ATCAGGTGGG 
ATTAATTCTG AAGATCAGGA TAATAGCCCA CAGCATAAAC AAAGTTGGAA TGTTCTGGAA 
AGTCAGGGAA AATCAAGAGA GAATAAGACA AACATAACAG AGCATATCAA AGGACAAACA 
GATTGTGAAG ACGTGTGGGG AAAAAGAGAT AATACGAGGA GTTTGAAAGC TACTACAGAA 
GAATTGTTTA CCTGCCAAGA AACAGTGTGC TGTGAACTGT CTTCTCTAGC TGATCATGGC 
ATTACTGAGA AAGCAGAAGC TGGTACAGCC TATATAATTA AGACAACATC AGAAAGTACT 
CCAGAAAGCA TGTCTGCTAG AGAAAAAGCA ATAATTGCTA AGCTACCTCA AGAGACAGCA 
CGAAGTGACA GGCCCATCGA GGTAAAGGAA ACAGCGTTTG ATCCACATGA AGGGAGAAAT 
GATGATTCAC ATTATACCCT TTGTCAACGA GATACAGTAG GTGTAATCTA TGACAATGAT 
TTTGAAAAGG AATCACGTTT AGGTATTTGT AATGTACGTG TAGATGAAAT GGAGAAGGAA 
GAAACCATGT CTATGTACAA TCCTAGGAAG ACACATGACA GGGAGAAATG TGGCACTGGA 
AATATAACAT CTGTGGAAGA ATCCTCATGG GTCATTACAG AATATCAAAA AGCAACTTCA 
AAACTGGATT TACAGTTGGG AATGTTACCA ACAGACAAAA CTGTATTTTC AGAAAACAGA 
GATCTTAGGC AGGTTCAAGA ATTATCAAAG AAAACAGACT CGGATGCCAT TGTGCATTCT 
GCTTTTAACT CAGACACTAA TAGAGCTCCT CAGAATAGCT CTCCTTTTTC CAAACATCAT 
ACTGAAATTT CAGTGTCAAC TAATGAGCAG GCAATTGCTG TAGAGAATGC AGTTACTACC 
ATGGCTAGCC AACCTATTTC TACGAAATCA GAAAATATTT GTAATTCAAC AAGAGAAATC 
CAGGGTATTG AGAAGCACCC TTATCCTGAG TCTAAACCTG AAGAAGTTTC CAGAAGTTCA 
GGAATAGTGA CATCAGGTAG TAGAAAAGAA AGATGCATAG GCCAGATTTT CCAAACAGAA 
GAGTATAGTG TGGAAAAATC TCTAGGGCCA ATGATTTTAA TCAACAAACC TCTTGAGAAT 
ATGGAAGAAG CAAGGCATGA AAATGAAGGA TTAGTAAGCT CTGGGCAATC ACTATACACT 
TCAGGTGAAA AGGAATCTGA CAGCTCTGCT TCTACTAGTC TTCCTGTTGA GGAAAGTCAA 
GCTCAAGGCA ACGAATCTTT GTTTTCAAAA TATACCAACT CTAAAATACC TTATTTCCTT 
TTGTTTCTGA TATTTCTTAT AACTGTCTAC CATTATGACT TAATGATTGG CTTGACATTC 
TACGTTTTGT CATTGTCCTG GCTATCCTGG GAAGAGGGTA GACAAAAAGA GTCTGTCAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002702.2
CDS	32..3400
Translation	MEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSGTRRVSFADSFGFNLVSVKEFDCWELPSASTTFDLGTDIFHTEEYVLAPLFDLPSSKEDLMQQLQIQKAILESTESLLGSTSIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKIVLVPPYQKDGSKVEFCIRYETSVGTFWSNNNGTNYTFICQKKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNTDTYIPTIICSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFPNKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLGDTSSDELVQLHTGSKEVLDDNANPAHGNGTVQIPCPSSDQLMAGNLNKKHEGGAKNIEVKDLGCLRRDFHSDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNRDLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDYLFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTEELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKETAFDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDLRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

Target ORF information:

RefSeq Version	NM_002711.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002711.3

ORF Insert Sequence:

ATGGAGCCTT CTGAAGTACC TAGTCAGATT AGCAAAGATA ATTTTTTAGA AGTTCCTAAT 
TTATCTGACT CTCTTTGTGA AGATGAAGAA GTTACTTTCC AACCTGGTTT CTCCCCTCAA 
CCAAGTAGAC GAGGTTCTGA TTCTTCTGAA GACATATACC TGGATACCCC ATCTTCAGGT 
ACTAGAAGAG TTTCATTTGC TGATTCCTTT GGATTCAATC TTGTGTCTGT TAAAGAATTT 
GATTGCTGGG AATTACCGAG TGCTTCAACC ACTTTTGACT TAGGGACGGA CATTTTCCAC 
ACAGAAGAAT ATGTTTTAGC CCCACTGTTT GACTTGCCTT CTTCAAAAGA AGATCTTATG 
CAACAACTCC AAATACAGAA AGCAATACTG GAGTCAACTG AGTCTCTTCT TGGGTCTACA 
AGTATCAAGG GTATTATTCG AGTTTTGAAT GTTTCTTTTG AGAAGTTAGT ATATGTAAGA 
ATGTCTTTAG ATGACTGGCA GACACATTAT GACATTTTAG CAGAATATGT TCCTAATTCA 
TGTGATGGTG AAACTGACCA GTTCTCCTTT AAGATTGTAT TGGTTCCTCC TTATCAAAAA 
GATGGCAGTA AAGTTGAGTT TTGTATACGT TATGAAACTT CTGTTGGTAC ATTTTGGTCA 
AATAATAATG GCACAAATTA TACATTCATT TGTCAAAAGA AAGAACAAGA GCCGGAGCCT 
GTAAAACCAT GGAAAGAAGT TCCTAACAGA CAAATAAAAG GCTGCTTAAA GGTAAAATCA 
AGTAAAGAAG AATCATCAGT AACATCAGAA GAAAATAACT TTGAGAATCC AAAGAATACA 
GATACCTATA TCCCAACAAT CATTTGTTCT CATGAGGACA AGGAAGATTT GGAAGCCAGT 
AATCGAAATG TAAAAGATGT AAACAGGGAA CATGATGAAC ATAATGAAAA AGAATTAGAG 
TTGATGATAA ATCAACACTT AATAAGAACC AGAAGTACTG CTTCCAGAGA TGAAAGGAAT 
ACATTTTCAA CAGATCCAGT CAATTTTCCA AATAAAGCAG AGGGGTTAGA GAAGAAGCAA 
ATCCATGGTG AAATATGTAC TGACTTGTTC CAAAGGTCTC TGTCTCCAAG TTCATCAGCA 
GAAAGCTCCG TAAAGGGAGA TTTTTACTGC AATGAAAAAT ATTCCTCAGG AGATGACTGT 
ACACATCAAC CTTCAGAGGA AACTACTTCA AATATGGGAG AAATCAAGCC ATCATTGGGA 
GATACTAGTA GTGATGAACT AGTGCAATTA CATACTGGCA GCAAAGAAGT CCTGGATGAT 
AATGCTAATC CAGCCCATGG CAATGGCACA GTGCAAATAC CTTGCCCCTC TTCAGATCAA 
CTAATGGCAG GAAACCTTAA TAAAAAACAT GAAGGAGGAG CTAAAAATAT TGAAGTAAAA 
GATTTGGGAT GTTTACGAAG AGATTTCCAT TCAGATACGT CGGCATGTCT CAAAGAATCA 
ACAGAAGAAG GATCTTCTAA GGAAGATTAT TATGGCAATG GTAAGGATGA TGAAGAACAA 
AGAATATATT TAGGTGTTAA TGAAAAACAA AGAAAAAATT TCCAAACAAT CTTACATGAC 
CAAGAAAGGA AGATGGGTAA CCCTAAAATA AGTGTGGCAG GGATTGGAGC TAGTAACAGA 
GACCTGGCTA CTCTGCTGAG CGAACATACC GCAATCCCCA CCCGGGCAAT CACAGCAGAT 
GTGTCTCATT CACCAAGGAC AAATTTAAGT TGGGAAGAAG CTGTGTTAAC CCCAGAGCAT 
CATCATTTGA CTAGTGAAGG CAGCGCTTTA GGAGGGATAA CTGGTCAAGT TTGTTCATCA 
AGAACTGGAA ATGTTTTGAG GAATGATTAT CTTTTCCAAG TTGAAGAAAA ATCAGGTGGG 
ATTAATTCTG AAGATCAGGA TAATAGCCCA CAGCATAAAC AAAGTTGGAA TGTTCTGGAA 
AGTCAGGGAA AATCAAGAGA GAATAAGACA AACATAACAG AGCATATCAA AGGACAAACA 
GATTGTGAAG ACGTGTGGGG AAAAAGAGAT AATACGAGGA GTTTGAAAGC TACTACAGAA 
GAATTGTTTA CCTGCCAAGA AACAGTGTGC TGTGAACTGT CTTCTCTAGC TGATCATGGC 
ATTACTGAGA AAGCAGAAGC TGGTACAGCC TATATAATTA AGACAACATC AGAAAGTACT 
CCAGAAAGCA TGTCTGCTAG AGAAAAAGCA ATAATTGCTA AGCTACCTCA AGAGACAGCA 
CGAAGTGACA GGCCCATCGA GGTAAAGGAA ACAGCGTTTG ATCCACATGA AGGGAGAAAT 
GATGATTCAC ATTATACCCT TTGTCAACGA GATACAGTAG GTGTAATCTA TGACAATGAT 
TTTGAAAAGG AATCACGTTT AGGTATTTGT AATGTACGTG TAGATGAAAT GGAGAAGGAA 
GAAACCATGT CTATGTACAA TCCTAGGAAG ACACATGACA GGGAGAAATG TGGCACTGGA 
AATATAACAT CTGTGGAAGA ATCCTCATGG GTCATTACAG AATATCAAAA AGCAACTTCA 
AAACTGGATT TACAGTTGGG AATGTTACCA ACAGACAAAA CTGTATTTTC AGAAAACAGA 
GATCTTAGGC AGGTTCAAGA ATTATCAAAG AAAACAGACT CGGATGCCAT TGTGCATTCT 
GCTTTTAACT CAGACACTAA TAGAGCTCCT CAGAATAGCT CTCCTTTTTC CAAACATCAT 
ACTGAAATTT CAGTGTCAAC TAATGAGCAG GCAATTGCTG TAGAGAATGC AGTTACTACC 
ATGGCTAGCC AACCTATTTC TACGAAATCA GAAAATATTT GTAATTCAAC AAGAGAAATC 
CAGGGTATTG AGAAGCACCC TTATCCTGAG TCTAAACCTG AAGAAGTTTC CAGAAGTTCA 
GGAATAGTGA CATCAGGTAG TAGAAAAGAA AGATGCATAG GCCAGATTTT CCAAACAGAA 
GAGTATAGTG TGGAAAAATC TCTAGGGCCA ATGATTTTAA TCAACAAACC TCTTGAGAAT 
ATGGAAGAAG CAAGGCATGA AAATGAAGGA TTAGTAAGCT CTGGGCAATC ACTATACACT 
TCAGGTGAAA AGGAATCTGA CAGCTCTGCT TCTACTAGTC TTCCTGTTGA GGAAAGTCAA 
GCTCAAGGCA ACGAATCTTT GTTTTCAAAA TATACCAACT CTAAAATACC TTATTTCCTT 
TTGTTTCTGA TATTTCTTAT AACTGTCTAC CATTATGACT TAATGATTGG CTTGACATTC 
TACGTTTTGT CATTGTCCTG GCTATCCTGG GAAGAGGGTA GACAAAAAGA GTCTGTCAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R3A ( NM_002711.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002702.2 Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), mRNA.

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CloneID	OHu17972
Clone ID Related Accession (Same CDS sequence)	NM_002711.3 , NM_002711.4
Accession Version	NM_002711.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577721600000
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 3A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC126451.1, AC092465.5, X78578.1 and DB527300.2. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_002711.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X78578.1, BC126451.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2149178 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000284601.4/ ENSP00000284601.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCTT CTGAAGTACC TAGTCAGATT AGCAAAGATA ATTTTTTAGA AGTTCCTAAT 
TTATCTGACT CTCTTTGTGA AGATGAAGAA GTTACTTTCC AACCTGGTTT CTCCCCTCAA 
CCAAGTAGAC GAGGTTCTGA TTCTTCTGAA GACATATACC TGGATACCCC ATCTTCAGGT 
ACTAGAAGAG TTTCATTTGC TGATTCCTTT GGATTCAATC TTGTGTCTGT TAAAGAATTT 
GATTGCTGGG AATTACCGAG TGCTTCAACC ACTTTTGACT TAGGGACGGA CATTTTCCAC 
ACAGAAGAAT ATGTTTTAGC CCCACTGTTT GACTTGCCTT CTTCAAAAGA AGATCTTATG 
CAACAACTCC AAATACAGAA AGCAATACTG GAGTCAACTG AGTCTCTTCT TGGGTCTACA 
AGTATCAAGG GTATTATTCG AGTTTTGAAT GTTTCTTTTG AGAAGTTAGT ATATGTAAGA 
ATGTCTTTAG ATGACTGGCA GACACATTAT GACATTTTAG CAGAATATGT TCCTAATTCA 
TGTGATGGTG AAACTGACCA GTTCTCCTTT AAGATTGTAT TGGTTCCTCC TTATCAAAAA 
GATGGCAGTA AAGTTGAGTT TTGTATACGT TATGAAACTT CTGTTGGTAC ATTTTGGTCA 
AATAATAATG GCACAAATTA TACATTCATT TGTCAAAAGA AAGAACAAGA GCCGGAGCCT 
GTAAAACCAT GGAAAGAAGT TCCTAACAGA CAAATAAAAG GCTGCTTAAA GGTAAAATCA 
AGTAAAGAAG AATCATCAGT AACATCAGAA GAAAATAACT TTGAGAATCC AAAGAATACA 
GATACCTATA TCCCAACAAT CATTTGTTCT CATGAGGACA AGGAAGATTT GGAAGCCAGT 
AATCGAAATG TAAAAGATGT AAACAGGGAA CATGATGAAC ATAATGAAAA AGAATTAGAG 
TTGATGATAA ATCAACACTT AATAAGAACC AGAAGTACTG CTTCCAGAGA TGAAAGGAAT 
ACATTTTCAA CAGATCCAGT CAATTTTCCA AATAAAGCAG AGGGGTTAGA GAAGAAGCAA 
ATCCATGGTG AAATATGTAC TGACTTGTTC CAAAGGTCTC TGTCTCCAAG TTCATCAGCA 
GAAAGCTCCG TAAAGGGAGA TTTTTACTGC AATGAAAAAT ATTCCTCAGG AGATGACTGT 
ACACATCAAC CTTCAGAGGA AACTACTTCA AATATGGGAG AAATCAAGCC ATCATTGGGA 
GATACTAGTA GTGATGAACT AGTGCAATTA CATACTGGCA GCAAAGAAGT CCTGGATGAT 
AATGCTAATC CAGCCCATGG CAATGGCACA GTGCAAATAC CTTGCCCCTC TTCAGATCAA 
CTAATGGCAG GAAACCTTAA TAAAAAACAT GAAGGAGGAG CTAAAAATAT TGAAGTAAAA 
GATTTGGGAT GTTTACGAAG AGATTTCCAT TCAGATACGT CGGCATGTCT CAAAGAATCA 
ACAGAAGAAG GATCTTCTAA GGAAGATTAT TATGGCAATG GTAAGGATGA TGAAGAACAA 
AGAATATATT TAGGTGTTAA TGAAAAACAA AGAAAAAATT TCCAAACAAT CTTACATGAC 
CAAGAAAGGA AGATGGGTAA CCCTAAAATA AGTGTGGCAG GGATTGGAGC TAGTAACAGA 
GACCTGGCTA CTCTGCTGAG CGAACATACC GCAATCCCCA CCCGGGCAAT CACAGCAGAT 
GTGTCTCATT CACCAAGGAC AAATTTAAGT TGGGAAGAAG CTGTGTTAAC CCCAGAGCAT 
CATCATTTGA CTAGTGAAGG CAGCGCTTTA GGAGGGATAA CTGGTCAAGT TTGTTCATCA 
AGAACTGGAA ATGTTTTGAG GAATGATTAT CTTTTCCAAG TTGAAGAAAA ATCAGGTGGG 
ATTAATTCTG AAGATCAGGA TAATAGCCCA CAGCATAAAC AAAGTTGGAA TGTTCTGGAA 
AGTCAGGGAA AATCAAGAGA GAATAAGACA AACATAACAG AGCATATCAA AGGACAAACA 
GATTGTGAAG ACGTGTGGGG AAAAAGAGAT AATACGAGGA GTTTGAAAGC TACTACAGAA 
GAATTGTTTA CCTGCCAAGA AACAGTGTGC TGTGAACTGT CTTCTCTAGC TGATCATGGC 
ATTACTGAGA AAGCAGAAGC TGGTACAGCC TATATAATTA AGACAACATC AGAAAGTACT 
CCAGAAAGCA TGTCTGCTAG AGAAAAAGCA ATAATTGCTA AGCTACCTCA AGAGACAGCA 
CGAAGTGACA GGCCCATCGA GGTAAAGGAA ACAGCGTTTG ATCCACATGA AGGGAGAAAT 
GATGATTCAC ATTATACCCT TTGTCAACGA GATACAGTAG GTGTAATCTA TGACAATGAT 
TTTGAAAAGG AATCACGTTT AGGTATTTGT AATGTACGTG TAGATGAAAT GGAGAAGGAA 
GAAACCATGT CTATGTACAA TCCTAGGAAG ACACATGACA GGGAGAAATG TGGCACTGGA 
AATATAACAT CTGTGGAAGA ATCCTCATGG GTCATTACAG AATATCAAAA AGCAACTTCA 
AAACTGGATT TACAGTTGGG AATGTTACCA ACAGACAAAA CTGTATTTTC AGAAAACAGA 
GATCTTAGGC AGGTTCAAGA ATTATCAAAG AAAACAGACT CGGATGCCAT TGTGCATTCT 
GCTTTTAACT CAGACACTAA TAGAGCTCCT CAGAATAGCT CTCCTTTTTC CAAACATCAT 
ACTGAAATTT CAGTGTCAAC TAATGAGCAG GCAATTGCTG TAGAGAATGC AGTTACTACC 
ATGGCTAGCC AACCTATTTC TACGAAATCA GAAAATATTT GTAATTCAAC AAGAGAAATC 
CAGGGTATTG AGAAGCACCC TTATCCTGAG TCTAAACCTG AAGAAGTTTC CAGAAGTTCA 
GGAATAGTGA CATCAGGTAG TAGAAAAGAA AGATGCATAG GCCAGATTTT CCAAACAGAA 
GAGTATAGTG TGGAAAAATC TCTAGGGCCA ATGATTTTAA TCAACAAACC TCTTGAGAAT 
ATGGAAGAAG CAAGGCATGA AAATGAAGGA TTAGTAAGCT CTGGGCAATC ACTATACACT 
TCAGGTGAAA AGGAATCTGA CAGCTCTGCT TCTACTAGTC TTCCTGTTGA GGAAAGTCAA 
GCTCAAGGCA ACGAATCTTT GTTTTCAAAA TATACCAACT CTAAAATACC TTATTTCCTT 
TTGTTTCTGA TATTTCTTAT AACTGTCTAC CATTATGACT TAATGATTGG CTTGACATTC 
TACGTTTTGT CATTGTCCTG GCTATCCTGG GAAGAGGGTA GACAAAAAGA GTCTGTCAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002702.2
CDS	14..3382
Translation	MEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSGTRRVSFADSFGFNLVSVKEFDCWELPSASTTFDLGTDIFHTEEYVLAPLFDLPSSKEDLMQQLQIQKAILESTESLLGSTSIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKIVLVPPYQKDGSKVEFCIRYETSVGTFWSNNNGTNYTFICQKKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNTDTYIPTIICSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFPNKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLGDTSSDELVQLHTGSKEVLDDNANPAHGNGTVQIPCPSSDQLMAGNLNKKHEGGAKNIEVKDLGCLRRDFHSDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNRDLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDYLFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTEELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKETAFDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDLRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

Target ORF information:

RefSeq Version	NM_002711.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 3A (PPP1R3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002711.4

ORF Insert Sequence:

ATGGAGCCTT CTGAAGTACC TAGTCAGATT AGCAAAGATA ATTTTTTAGA AGTTCCTAAT 
TTATCTGACT CTCTTTGTGA AGATGAAGAA GTTACTTTCC AACCTGGTTT CTCCCCTCAA 
CCAAGTAGAC GAGGTTCTGA TTCTTCTGAA GACATATACC TGGATACCCC ATCTTCAGGT 
ACTAGAAGAG TTTCATTTGC TGATTCCTTT GGATTCAATC TTGTGTCTGT TAAAGAATTT 
GATTGCTGGG AATTACCGAG TGCTTCAACC ACTTTTGACT TAGGGACGGA CATTTTCCAC 
ACAGAAGAAT ATGTTTTAGC CCCACTGTTT GACTTGCCTT CTTCAAAAGA AGATCTTATG 
CAACAACTCC AAATACAGAA AGCAATACTG GAGTCAACTG AGTCTCTTCT TGGGTCTACA 
AGTATCAAGG GTATTATTCG AGTTTTGAAT GTTTCTTTTG AGAAGTTAGT ATATGTAAGA 
ATGTCTTTAG ATGACTGGCA GACACATTAT GACATTTTAG CAGAATATGT TCCTAATTCA 
TGTGATGGTG AAACTGACCA GTTCTCCTTT AAGATTGTAT TGGTTCCTCC TTATCAAAAA 
GATGGCAGTA AAGTTGAGTT TTGTATACGT TATGAAACTT CTGTTGGTAC ATTTTGGTCA 
AATAATAATG GCACAAATTA TACATTCATT TGTCAAAAGA AAGAACAAGA GCCGGAGCCT 
GTAAAACCAT GGAAAGAAGT TCCTAACAGA CAAATAAAAG GCTGCTTAAA GGTAAAATCA 
AGTAAAGAAG AATCATCAGT AACATCAGAA GAAAATAACT TTGAGAATCC AAAGAATACA 
GATACCTATA TCCCAACAAT CATTTGTTCT CATGAGGACA AGGAAGATTT GGAAGCCAGT 
AATCGAAATG TAAAAGATGT AAACAGGGAA CATGATGAAC ATAATGAAAA AGAATTAGAG 
TTGATGATAA ATCAACACTT AATAAGAACC AGAAGTACTG CTTCCAGAGA TGAAAGGAAT 
ACATTTTCAA CAGATCCAGT CAATTTTCCA AATAAAGCAG AGGGGTTAGA GAAGAAGCAA 
ATCCATGGTG AAATATGTAC TGACTTGTTC CAAAGGTCTC TGTCTCCAAG TTCATCAGCA 
GAAAGCTCCG TAAAGGGAGA TTTTTACTGC AATGAAAAAT ATTCCTCAGG AGATGACTGT 
ACACATCAAC CTTCAGAGGA AACTACTTCA AATATGGGAG AAATCAAGCC ATCATTGGGA 
GATACTAGTA GTGATGAACT AGTGCAATTA CATACTGGCA GCAAAGAAGT CCTGGATGAT 
AATGCTAATC CAGCCCATGG CAATGGCACA GTGCAAATAC CTTGCCCCTC TTCAGATCAA 
CTAATGGCAG GAAACCTTAA TAAAAAACAT GAAGGAGGAG CTAAAAATAT TGAAGTAAAA 
GATTTGGGAT GTTTACGAAG AGATTTCCAT TCAGATACGT CGGCATGTCT CAAAGAATCA 
ACAGAAGAAG GATCTTCTAA GGAAGATTAT TATGGCAATG GTAAGGATGA TGAAGAACAA 
AGAATATATT TAGGTGTTAA TGAAAAACAA AGAAAAAATT TCCAAACAAT CTTACATGAC 
CAAGAAAGGA AGATGGGTAA CCCTAAAATA AGTGTGGCAG GGATTGGAGC TAGTAACAGA 
GACCTGGCTA CTCTGCTGAG CGAACATACC GCAATCCCCA CCCGGGCAAT CACAGCAGAT 
GTGTCTCATT CACCAAGGAC AAATTTAAGT TGGGAAGAAG CTGTGTTAAC CCCAGAGCAT 
CATCATTTGA CTAGTGAAGG CAGCGCTTTA GGAGGGATAA CTGGTCAAGT TTGTTCATCA 
AGAACTGGAA ATGTTTTGAG GAATGATTAT CTTTTCCAAG TTGAAGAAAA ATCAGGTGGG 
ATTAATTCTG AAGATCAGGA TAATAGCCCA CAGCATAAAC AAAGTTGGAA TGTTCTGGAA 
AGTCAGGGAA AATCAAGAGA GAATAAGACA AACATAACAG AGCATATCAA AGGACAAACA 
GATTGTGAAG ACGTGTGGGG AAAAAGAGAT AATACGAGGA GTTTGAAAGC TACTACAGAA 
GAATTGTTTA CCTGCCAAGA AACAGTGTGC TGTGAACTGT CTTCTCTAGC TGATCATGGC 
ATTACTGAGA AAGCAGAAGC TGGTACAGCC TATATAATTA AGACAACATC AGAAAGTACT 
CCAGAAAGCA TGTCTGCTAG AGAAAAAGCA ATAATTGCTA AGCTACCTCA AGAGACAGCA 
CGAAGTGACA GGCCCATCGA GGTAAAGGAA ACAGCGTTTG ATCCACATGA AGGGAGAAAT 
GATGATTCAC ATTATACCCT TTGTCAACGA GATACAGTAG GTGTAATCTA TGACAATGAT 
TTTGAAAAGG AATCACGTTT AGGTATTTGT AATGTACGTG TAGATGAAAT GGAGAAGGAA 
GAAACCATGT CTATGTACAA TCCTAGGAAG ACACATGACA GGGAGAAATG TGGCACTGGA 
AATATAACAT CTGTGGAAGA ATCCTCATGG GTCATTACAG AATATCAAAA AGCAACTTCA 
AAACTGGATT TACAGTTGGG AATGTTACCA ACAGACAAAA CTGTATTTTC AGAAAACAGA 
GATCTTAGGC AGGTTCAAGA ATTATCAAAG AAAACAGACT CGGATGCCAT TGTGCATTCT 
GCTTTTAACT CAGACACTAA TAGAGCTCCT CAGAATAGCT CTCCTTTTTC CAAACATCAT 
ACTGAAATTT CAGTGTCAAC TAATGAGCAG GCAATTGCTG TAGAGAATGC AGTTACTACC 
ATGGCTAGCC AACCTATTTC TACGAAATCA GAAAATATTT GTAATTCAAC AAGAGAAATC 
CAGGGTATTG AGAAGCACCC TTATCCTGAG TCTAAACCTG AAGAAGTTTC CAGAAGTTCA 
GGAATAGTGA CATCAGGTAG TAGAAAAGAA AGATGCATAG GCCAGATTTT CCAAACAGAA 
GAGTATAGTG TGGAAAAATC TCTAGGGCCA ATGATTTTAA TCAACAAACC TCTTGAGAAT 
ATGGAAGAAG CAAGGCATGA AAATGAAGGA TTAGTAAGCT CTGGGCAATC ACTATACACT 
TCAGGTGAAA AGGAATCTGA CAGCTCTGCT TCTACTAGTC TTCCTGTTGA GGAAAGTCAA 
GCTCAAGGCA ACGAATCTTT GTTTTCAAAA TATACCAACT CTAAAATACC TTATTTCCTT 
TTGTTTCTGA TATTTCTTAT AACTGTCTAC CATTATGACT TAATGATTGG CTTGACATTC 
TACGTTTTGT CATTGTCCTG GCTATCCTGG GAAGAGGGTA GACAAAAAGA GTCTGTCAAA 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pathologic gene network rewiring implicates PPP1R3A as a central regulator in pressure overload heart failure.
Nature communications10(1)2760(2019 06)
Cordero P,Parikh VN,Chin ET,Erbilgin A,Gloudemans MJ,Shang C,Huang Y,Chang AC,Smith KS,Dewey F,Zaleta K,Morley M,Brandimarto J,Glazer N,Waggott D,Pavlovic A,Zhao M,Moravec CS,Tang WHW,Skreen J,Malloy C,Hannenhalli S,Li H,Ritter S,Li M,Bernstein D,Connolly A,Hakonarson H,Lusis AJ,Margulies KB,Depaoli-Roach AA,Montgomery SB,Wheeler MT,Cappola T,Ashley EA

Whole-exome sequencing in maya indigenous families: variant in PPP1R3A is associated with type 2 diabetes.
Molecular genetics and genomics : MGG293(5)1205-1216(2018 Oct)
Sánchez-Pozos K,Ortíz-López MG,Peña-Espinoza BI,de Los Ángeles Granados-Silvestre M,Jiménez-Jacinto V,Verleyen J,Tekola-Ayele F,Sanchez-Flores A,Menjivar M

Molecular Differences between Screen-Detected and Interval Breast Cancers Are Largely Explained by PAM50 Subtypes.
Clinical cancer research : an official journal of the American Association for Cancer Research23(10)2584-2592(2017 May)
Li J,Ivansson E,Klevebring D,Tobin NP,Lindström LS,Holm J,Prochazka G,Cristando C,Palmgren J,Törnberg S,Humphreys K,Hartman J,Frisell J,Rantalainen M,Lindberg J,Hall P,Bergh J,Grönberg H,Czene K

Gene-smoking interactions identify several novel blood pressure loci in the Framingham Heart Study.
American journal of hypertension28(3)343-54(2015 Mar)
Sung YJ,de Las Fuentes L,Schwander KL,Simino J,Rao DC

A novel interaction between hScrib and PP1γ downregulates ERK signaling and suppresses oncogene-induced cell transformation.
PloS one8(1)e53752(2013)
Nagasaka K,Seiki T,Yamashita A,Massimi P,Subbaiah VK,Thomas M,Kranjec C,Kawana K,Nakagawa S,Yano T,Taketani Y,Fujii T,Kozuma S,Banks L

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