PDK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDK2
Entrez Gene ID	5164
Full Name	pyruvate dehydrogenase kinase 2
Synonyms	PDHK2,PDKII
General protein information	Preferred Names pyruvate dehydrogenase kinase 2 Names pyruvate dehydrogenase kinase, isozyme 2 PDH kinase 2 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial pyruvate dehydrogenase kinase, isoenzyme 2 pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.33
Summary	This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010].
Disorder MIM:	602525

mRNA and Protein(s)

mRNA	Protein	Name
NM_002611.4	NP_002602.2	pyruvate dehydrogenase kinase, isozyme 2 isoform 1 precursor
NM_002611.5	NP_002602.2	pyruvate dehydrogenase kinase, isozyme 2 isoform 1 precursor
NM_001199898.1	NP_001186827.1	pyruvate dehydrogenase kinase, isozyme 2 isoform 2
NM_001199899.1	NP_001186828.1	pyruvate dehydrogenase kinase, isozyme 2 isoform 2
NM_001199900.1	NP_001186829.1	pyruvate dehydrogenase kinase, isozyme 2 isoform 3 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PDK2	XP_005624690.1
Mus musculus (house mouse)	Pdk2	NP_598428.2
Rattus norvegicus (Norway rat)	Pdk2	NP_110499.1
Pan troglodytes (chimpanzee)	PDK2	XP_523791.3
Xenopus tropicalis (tropical clawed frog)	pdk4	NP_001006803.1
Bos taurus (cattle)	PDK2	NP_001152953.1
Danio rerio (zebrafish)	si:dkeyp-67g8.1	XP_005156305.1
Danio rerio (zebrafish)	pdk2	NP_957290.1
Homo sapiens (human)	PDK2	NP_002602.2

Gene Ontology
Bibliography

Related articles in PubMed

MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer.
He Z, Li Z, Zhang X, Yin K, Wang W, Xu Z, Li B, Zhang L, Xu J, Sun G, Wang L, Li Q, Huang X, Zhang L, Zhang D, Xu H, Xu Z
Cell death & disease9(5)505(2018 05)

The Mitochondrial Rhomboid Protease PARL Is Regulated by PDK2 to Integrate Mitochondrial Quality Control and Metabolism.
Shi G, McQuibban GA
Cell reports18(6)1458-1472(2017 02)

PDK2 and ABCG2 genes polymorphisms are correlated with blood glucose levels and uric acid in Tibetan gout patients.
Ren YC, Jin TB, Sun XD, Geng TT, Zhang MX, Wang L, Feng T, Kang LL, Chen C
Genetics and molecular research : GMR15(1)(2016 Feb)

Activation of mitochondrial oxidation by PDK2 inhibition reverses cisplatin resistance in head and neck cancer.
Roh JL, Park JY, Kim EH, Jang HJ, Kwon M
Cancer letters371(1)20-9(2016 Feb)

PDK2-mediated alternative splicing switches Bnip3 from cell death to cell survival.
Gang H, Dhingra R, Lin J, Hai Y, Aviv Y, Margulets V, Hamedani M, Thanasupawat T, Leygue E, Klonisch T, Davie JR, Kirshenbaum LA
The Journal of cell biology210(7)1101-15(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21731	NM_001199900.1 Latest version!	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu20348	NM_001199898.1 Latest version!	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu20348	NM_001199899.1 Latest version!	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu21237	NM_002611.5 Latest version!	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu21237	NM_002611.4	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDK2 ( NM_001199900.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186829.1 Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 4, mRNA.

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CloneID	OHu21731
Clone ID Related Accession (Same CDS sequence)	NM_001199900.1
Accession Version	NM_001199900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 2 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002401.2, BQ071206.1, AW302405.1 and AW469593.1. Transcript Variant: This variant (4) lacks a splice site in the 3' coding region, compared to variant 1. This results in a shorter protein with a distinct C-terminus (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.244053.1, SRR1163655.135818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTGGG TGTGGGCGCT GCTGAAGAAT GCGTCCCTGG CAGGGGCGCC CAAGTACATA 
GAGCACTTCA GCAAGTTCTC CCCGTCCCCG CTGTCCATGA AGCAGTTTCT GGACTTCGGA 
TCCAGCAATG CCTGTGAGAA AACCTCCTTC ACCTTCCTCA GGCAGGAGCT GCCTGTGCGC 
CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCCGACC GAGTGCTGAG CACACCCTCC 
GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGCCTCCTGG ACATCATGGA GTTCCTGGAC 
AAGGATCCCG AGGACCATCG CACCCTGAGC CAGTTCACTG ACGCCCTGGT CACCATCCGG 
AACCGGCACA ACGACGTGGT GCCCACCATG GCACAAGGCG TGCTTGAGTA CAAGGACACC 
TACGGCGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC 
AGCCGCATCT CCATCCGCAT GCTCATCAAC CAGCACAGTG GGTGCCGGCC ACAGCGGCGG 
GGAGCGGGCG GTGGGGGGGG CGGTGCTGGG GCCCAGGGCC GGGCTGCTGA GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186829.1
CDS	162..761
Translation	MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHSGCRPQRRGAGGGGGGAGAQGRAAEGT

Target ORF information:

RefSeq Version	NM_001199900.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199900.1

ORF Insert Sequence:

ATGCGCTGGG TGTGGGCGCT GCTGAAGAAT GCGTCCCTGG CAGGGGCGCC CAAGTACATA 
GAGCACTTCA GCAAGTTCTC CCCGTCCCCG CTGTCCATGA AGCAGTTTCT GGACTTCGGA 
TCCAGCAATG CCTGTGAGAA AACCTCCTTC ACCTTCCTCA GGCAGGAGCT GCCTGTGCGC 
CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCCGACC GAGTGCTGAG CACACCCTCC 
GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGCCTCCTGG ACATCATGGA GTTCCTGGAC 
AAGGATCCCG AGGACCATCG CACCCTGAGC CAGTTCACTG ACGCCCTGGT CACCATCCGG 
AACCGGCACA ACGACGTGGT GCCCACCATG GCACAAGGCG TGCTTGAGTA CAAGGACACC 
TACGGCGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC 
AGCCGCATCT CCATCCGCAT GCTCATCAAC CAGCACAGTG GGTGCCGGCC ACAGCGGCGG 
GGAGCGGGCG GTGGGGGGGG CGGTGCTGGG GCCCAGGGCC GGGCTGCTGA GGGGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDK2 ( NM_001199898.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186827.1 Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20348
Clone ID Related Accession (Same CDS sequence)	NM_001199898.1 , NM_001199899.1
Accession Version	NM_001199898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK055119.1 and BQ024783.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK055119.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAGAGA TCAACCTGCT TCCCGACCGA GTGCTGAGCA CACCCTCCGT GCAGCTGGTG 
CAGAGCTGGT ATGTCCAGAG CCTCCTGGAC ATCATGGAGT TCCTGGACAA GGATCCCGAG 
GACCATCGCA CCCTGAGCCA GTTCACTGAC GCCCTGGTCA CCATCCGGAA CCGGCACAAC 
GACGTGGTGC CCACCATGGC ACAAGGCGTG CTTGAGTACA AGGACACCTA CGGCGATGAC 
CCCGTCTCCA ACCAGAACAT CCAGTACTTC CTGGACCGCT TCTACCTCAG CCGCATCTCC 
ATCCGCATGC TCATCAACCA GCACACCCTC ATCTTTGATG GCAGCACCAA CCCAGCCCAT 
CCCAAACACA TCGGCAGCAT CGACCCCAAC TGCAACGTCT CTGAGGTGGT CAAAGATGCC 
TACGACATGG CTAAGCTCCT GTGTGACAAG TATTACATGG CCTCACCTGA CCTGGAGATC 
CAGGAGATCA ATGCAGCCAA CTCCAAACAG CCGATTCACA TGGTCTACGT CCCCTCCCAC 
CTCTACCACA TGCTCTTTGA GCTCTTCAAG AATGCCATGA GGGCGACTGT GGAAAGCCAT 
GAGTCCAGCC TCATTCTCCC ACCCATCAAG GTCATGGTGG CCTTGGGTGA GGAAGATCTG 
TCCATCAAGA TGAGTGACCG AGGTGGGGGT GTTCCCTTGA GGAAGATTGA GCGACTCTTC 
AGCTACATGT ACTCCACAGC ACCCACCCCC CAGCCTGGCA CCGGGGGAAC GCCGCTGGCT 
GGCTTTGGTT ATGGGCTCCC CATTTCCCGC CTCTACGCCA AGTACTTCCA GGGAGACCTG 
CAGCTCTTCT CCATGGAAGG CTTTGGGACC GATGCTGTCA TCTATCTCAA GGCCCTGTCC 
ACGGACTCGG TGGAGCGCCT GCCTGTCTAC AACAAGTCAG CCTGGCGCCA CTACCAGACC 
ATCCAGGAGG CCGGCGACTG GTGTGTGCCC AGCACGGAGC CCAAGAACAC GTCCACGTAC 
CGCGTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186827.1
CDS	392..1423
Translation	MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS

Target ORF information:

RefSeq Version	NM_001199898.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199898.1

ORF Insert Sequence:

ATGAAAGAGA TCAACCTGCT TCCCGACCGA GTGCTGAGCA CACCCTCCGT GCAGCTGGTG 
CAGAGCTGGT ATGTCCAGAG CCTCCTGGAC ATCATGGAGT TCCTGGACAA GGATCCCGAG 
GACCATCGCA CCCTGAGCCA GTTCACTGAC GCCCTGGTCA CCATCCGGAA CCGGCACAAC 
GACGTGGTGC CCACCATGGC ACAAGGCGTG CTTGAGTACA AGGACACCTA CGGCGATGAC 
CCCGTCTCCA ACCAGAACAT CCAGTACTTC CTGGACCGCT TCTACCTCAG CCGCATCTCC 
ATCCGCATGC TCATCAACCA GCACACCCTC ATCTTTGATG GCAGCACCAA CCCAGCCCAT 
CCCAAACACA TCGGCAGCAT CGACCCCAAC TGCAACGTCT CTGAGGTGGT CAAAGATGCC 
TACGACATGG CTAAGCTCCT GTGTGACAAG TATTACATGG CCTCACCTGA CCTGGAGATC 
CAGGAGATCA ATGCAGCCAA CTCCAAACAG CCGATTCACA TGGTCTACGT CCCCTCCCAC 
CTCTACCACA TGCTCTTTGA GCTCTTCAAG AATGCCATGA GGGCGACTGT GGAAAGCCAT 
GAGTCCAGCC TCATTCTCCC ACCCATCAAG GTCATGGTGG CCTTGGGTGA GGAAGATCTG 
TCCATCAAGA TGAGTGACCG AGGTGGGGGT GTTCCCTTGA GGAAGATTGA GCGACTCTTC 
AGCTACATGT ACTCCACAGC ACCCACCCCC CAGCCTGGCA CCGGGGGAAC GCCGCTGGCT 
GGCTTTGGTT ATGGGCTCCC CATTTCCCGC CTCTACGCCA AGTACTTCCA GGGAGACCTG 
CAGCTCTTCT CCATGGAAGG CTTTGGGACC GATGCTGTCA TCTATCTCAA GGCCCTGTCC 
ACGGACTCGG TGGAGCGCCT GCCTGTCTAC AACAAGTCAG CCTGGCGCCA CTACCAGACC 
ATCCAGGAGG CCGGCGACTG GTGTGTGCCC AGCACGGAGC CCAAGAACAC GTCCACGTAC 
CGCGTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDK2 ( NM_001199899.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186828.1 Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20348
Clone ID Related Accession (Same CDS sequence)	NM_001199898.1 , NM_001199899.1
Accession Version	NM_001199899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA199055.1, AC002401.2, BC040478.1 and BQ024783.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.22444.1, SRR1803615.56862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAGAGA TCAACCTGCT TCCCGACCGA GTGCTGAGCA CACCCTCCGT GCAGCTGGTG 
CAGAGCTGGT ATGTCCAGAG CCTCCTGGAC ATCATGGAGT TCCTGGACAA GGATCCCGAG 
GACCATCGCA CCCTGAGCCA GTTCACTGAC GCCCTGGTCA CCATCCGGAA CCGGCACAAC 
GACGTGGTGC CCACCATGGC ACAAGGCGTG CTTGAGTACA AGGACACCTA CGGCGATGAC 
CCCGTCTCCA ACCAGAACAT CCAGTACTTC CTGGACCGCT TCTACCTCAG CCGCATCTCC 
ATCCGCATGC TCATCAACCA GCACACCCTC ATCTTTGATG GCAGCACCAA CCCAGCCCAT 
CCCAAACACA TCGGCAGCAT CGACCCCAAC TGCAACGTCT CTGAGGTGGT CAAAGATGCC 
TACGACATGG CTAAGCTCCT GTGTGACAAG TATTACATGG CCTCACCTGA CCTGGAGATC 
CAGGAGATCA ATGCAGCCAA CTCCAAACAG CCGATTCACA TGGTCTACGT CCCCTCCCAC 
CTCTACCACA TGCTCTTTGA GCTCTTCAAG AATGCCATGA GGGCGACTGT GGAAAGCCAT 
GAGTCCAGCC TCATTCTCCC ACCCATCAAG GTCATGGTGG CCTTGGGTGA GGAAGATCTG 
TCCATCAAGA TGAGTGACCG AGGTGGGGGT GTTCCCTTGA GGAAGATTGA GCGACTCTTC 
AGCTACATGT ACTCCACAGC ACCCACCCCC CAGCCTGGCA CCGGGGGAAC GCCGCTGGCT 
GGCTTTGGTT ATGGGCTCCC CATTTCCCGC CTCTACGCCA AGTACTTCCA GGGAGACCTG 
CAGCTCTTCT CCATGGAAGG CTTTGGGACC GATGCTGTCA TCTATCTCAA GGCCCTGTCC 
ACGGACTCGG TGGAGCGCCT GCCTGTCTAC AACAAGTCAG CCTGGCGCCA CTACCAGACC 
ATCCAGGAGG CCGGCGACTG GTGTGTGCCC AGCACGGAGC CCAAGAACAC GTCCACGTAC 
CGCGTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186828.1
CDS	191..1222
Translation	MKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS

Target ORF information:

RefSeq Version	NM_001199899.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199899.1

ORF Insert Sequence:

ATGAAAGAGA TCAACCTGCT TCCCGACCGA GTGCTGAGCA CACCCTCCGT GCAGCTGGTG 
CAGAGCTGGT ATGTCCAGAG CCTCCTGGAC ATCATGGAGT TCCTGGACAA GGATCCCGAG 
GACCATCGCA CCCTGAGCCA GTTCACTGAC GCCCTGGTCA CCATCCGGAA CCGGCACAAC 
GACGTGGTGC CCACCATGGC ACAAGGCGTG CTTGAGTACA AGGACACCTA CGGCGATGAC 
CCCGTCTCCA ACCAGAACAT CCAGTACTTC CTGGACCGCT TCTACCTCAG CCGCATCTCC 
ATCCGCATGC TCATCAACCA GCACACCCTC ATCTTTGATG GCAGCACCAA CCCAGCCCAT 
CCCAAACACA TCGGCAGCAT CGACCCCAAC TGCAACGTCT CTGAGGTGGT CAAAGATGCC 
TACGACATGG CTAAGCTCCT GTGTGACAAG TATTACATGG CCTCACCTGA CCTGGAGATC 
CAGGAGATCA ATGCAGCCAA CTCCAAACAG CCGATTCACA TGGTCTACGT CCCCTCCCAC 
CTCTACCACA TGCTCTTTGA GCTCTTCAAG AATGCCATGA GGGCGACTGT GGAAAGCCAT 
GAGTCCAGCC TCATTCTCCC ACCCATCAAG GTCATGGTGG CCTTGGGTGA GGAAGATCTG 
TCCATCAAGA TGAGTGACCG AGGTGGGGGT GTTCCCTTGA GGAAGATTGA GCGACTCTTC 
AGCTACATGT ACTCCACAGC ACCCACCCCC CAGCCTGGCA CCGGGGGAAC GCCGCTGGCT 
GGCTTTGGTT ATGGGCTCCC CATTTCCCGC CTCTACGCCA AGTACTTCCA GGGAGACCTG 
CAGCTCTTCT CCATGGAAGG CTTTGGGACC GATGCTGTCA TCTATCTCAA GGCCCTGTCC 
ACGGACTCGG TGGAGCGCCT GCCTGTCTAC AACAAGTCAG CCTGGCGCCA CTACCAGACC 
ATCCAGGAGG CCGGCGACTG GTGTGTGCCC AGCACGGAGC CCAAGAACAC GTCCACGTAC 
CGCGTCAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDK2 ( NM_002611.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002602.2 Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21237
Clone ID Related Accession (Same CDS sequence)	NM_002611.4 , NM_002611.5
Accession Version	NM_002611.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002401.2, BC040478.1 and BQ024783.1. On Dec 30, 2010 this sequence version replaced NM_002611.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.174608.1, SRR1803612.191352.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTGGG TGTGGGCGCT GCTGAAGAAT GCGTCCCTGG CAGGGGCGCC CAAGTACATA 
GAGCACTTCA GCAAGTTCTC CCCGTCCCCG CTGTCCATGA AGCAGTTTCT GGACTTCGGA 
TCCAGCAATG CCTGTGAGAA AACCTCCTTC ACCTTCCTCA GGCAGGAGCT GCCTGTGCGC 
CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCCGACC GAGTGCTGAG CACACCCTCC 
GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGCCTCCTGG ACATCATGGA GTTCCTGGAC 
AAGGATCCCG AGGACCATCG CACCCTGAGC CAGTTCACTG ACGCCCTGGT CACCATCCGG 
AACCGGCACA ACGACGTGGT GCCCACCATG GCACAAGGCG TGCTTGAGTA CAAGGACACC 
TACGGCGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC 
AGCCGCATCT CCATCCGCAT GCTCATCAAC CAGCACACCC TCATCTTTGA TGGCAGCACC 
AACCCAGCCC ATCCCAAACA CATCGGCAGC ATCGACCCCA ACTGCAACGT CTCTGAGGTG 
GTCAAAGATG CCTACGACAT GGCTAAGCTC CTGTGTGACA AGTATTACAT GGCCTCACCT 
GACCTGGAGA TCCAGGAGAT CAATGCAGCC AACTCCAAAC AGCCGATTCA CATGGTCTAC 
GTCCCCTCCC ACCTCTACCA CATGCTCTTT GAGCTCTTCA AGAATGCCAT GAGGGCGACT 
GTGGAAAGCC ATGAGTCCAG CCTCATTCTC CCACCCATCA AGGTCATGGT GGCCTTGGGT 
GAGGAAGATC TGTCCATCAA GATGAGTGAC CGAGGTGGGG GTGTTCCCTT GAGGAAGATT 
GAGCGACTCT TCAGCTACAT GTACTCCACA GCACCCACCC CCCAGCCTGG CACCGGGGGA 
ACGCCGCTGG CTGGCTTTGG TTATGGGCTC CCCATTTCCC GCCTCTACGC CAAGTACTTC 
CAGGGAGACC TGCAGCTCTT CTCCATGGAA GGCTTTGGGA CCGATGCTGT CATCTATCTC 
AAGGCCCTGT CCACGGACTC GGTGGAGCGC CTGCCTGTCT ACAACAAGTC AGCCTGGCGC 
CACTACCAGA CCATCCAGGA GGCCGGCGAC TGGTGTGTGC CCAGCACGGA GCCCAAGAAC 
ACGTCCACGT ACCGCGTCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002602.2
CDS	162..1385
Translation	MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS

Target ORF information:

RefSeq Version	NM_002611.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002611.4

ORF Insert Sequence:

ATGCGCTGGG TGTGGGCGCT GCTGAAGAAT GCGTCCCTGG CAGGGGCGCC CAAGTACATA 
GAGCACTTCA GCAAGTTCTC CCCGTCCCCG CTGTCCATGA AGCAGTTTCT GGACTTCGGA 
TCCAGCAATG CCTGTGAGAA AACCTCCTTC ACCTTCCTCA GGCAGGAGCT GCCTGTGCGC 
CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCCGACC GAGTGCTGAG CACACCCTCC 
GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGCCTCCTGG ACATCATGGA GTTCCTGGAC 
AAGGATCCCG AGGACCATCG CACCCTGAGC CAGTTCACTG ACGCCCTGGT CACCATCCGG 
AACCGGCACA ACGACGTGGT GCCCACCATG GCACAAGGCG TGCTTGAGTA CAAGGACACC 
TACGGCGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC 
AGCCGCATCT CCATCCGCAT GCTCATCAAC CAGCACACCC TCATCTTTGA TGGCAGCACC 
AACCCAGCCC ATCCCAAACA CATCGGCAGC ATCGACCCCA ACTGCAACGT CTCTGAGGTG 
GTCAAAGATG CCTACGACAT GGCTAAGCTC CTGTGTGACA AGTATTACAT GGCCTCACCT 
GACCTGGAGA TCCAGGAGAT CAATGCAGCC AACTCCAAAC AGCCGATTCA CATGGTCTAC 
GTCCCCTCCC ACCTCTACCA CATGCTCTTT GAGCTCTTCA AGAATGCCAT GAGGGCGACT 
GTGGAAAGCC ATGAGTCCAG CCTCATTCTC CCACCCATCA AGGTCATGGT GGCCTTGGGT 
GAGGAAGATC TGTCCATCAA GATGAGTGAC CGAGGTGGGG GTGTTCCCTT GAGGAAGATT 
GAGCGACTCT TCAGCTACAT GTACTCCACA GCACCCACCC CCCAGCCTGG CACCGGGGGA 
ACGCCGCTGG CTGGCTTTGG TTATGGGCTC CCCATTTCCC GCCTCTACGC CAAGTACTTC 
CAGGGAGACC TGCAGCTCTT CTCCATGGAA GGCTTTGGGA CCGATGCTGT CATCTATCTC 
AAGGCCCTGT CCACGGACTC GGTGGAGCGC CTGCCTGTCT ACAACAAGTC AGCCTGGCGC 
CACTACCAGA CCATCCAGGA GGCCGGCGAC TGGTGTGTGC CCAGCACGGA GCCCAAGAAC 
ACGTCCACGT ACCGCGTCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDK2 ( NM_002611.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002602.2 Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 1, mRNA.

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CloneID	OHu21237
Clone ID Related Accession (Same CDS sequence)	NM_002611.4 , NM_002611.5
Accession Version	NM_002611.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575302400000
Organism	Homo sapiens(human)
Product	pyruvate dehydrogenase kinase, isozyme 2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002401.2, BC040478.1 and BQ024783.1. On May 17, 2019 this sequence version replaced NM_002611.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.21464.1, SRR1803613.61486.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000503176.6/ ENSP00000420927.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGCTGGG TGTGGGCGCT GCTGAAGAAT GCGTCCCTGG CAGGGGCGCC CAAGTACATA 
GAGCACTTCA GCAAGTTCTC CCCGTCCCCG CTGTCCATGA AGCAGTTTCT GGACTTCGGA 
TCCAGCAATG CCTGTGAGAA AACCTCCTTC ACCTTCCTCA GGCAGGAGCT GCCTGTGCGC 
CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCCGACC GAGTGCTGAG CACACCCTCC 
GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGCCTCCTGG ACATCATGGA GTTCCTGGAC 
AAGGATCCCG AGGACCATCG CACCCTGAGC CAGTTCACTG ACGCCCTGGT CACCATCCGG 
AACCGGCACA ACGACGTGGT GCCCACCATG GCACAAGGCG TGCTTGAGTA CAAGGACACC 
TACGGCGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC 
AGCCGCATCT CCATCCGCAT GCTCATCAAC CAGCACACCC TCATCTTTGA TGGCAGCACC 
AACCCAGCCC ATCCCAAACA CATCGGCAGC ATCGACCCCA ACTGCAACGT CTCTGAGGTG 
GTCAAAGATG CCTACGACAT GGCTAAGCTC CTGTGTGACA AGTATTACAT GGCCTCACCT 
GACCTGGAGA TCCAGGAGAT CAATGCAGCC AACTCCAAAC AGCCGATTCA CATGGTCTAC 
GTCCCCTCCC ACCTCTACCA CATGCTCTTT GAGCTCTTCA AGAATGCCAT GAGGGCGACT 
GTGGAAAGCC ATGAGTCCAG CCTCATTCTC CCACCCATCA AGGTCATGGT GGCCTTGGGT 
GAGGAAGATC TGTCCATCAA GATGAGTGAC CGAGGTGGGG GTGTTCCCTT GAGGAAGATT 
GAGCGACTCT TCAGCTACAT GTACTCCACA GCACCCACCC CCCAGCCTGG CACCGGGGGA 
ACGCCGCTGG CTGGCTTTGG TTATGGGCTC CCCATTTCCC GCCTCTACGC CAAGTACTTC 
CAGGGAGACC TGCAGCTCTT CTCCATGGAA GGCTTTGGGA CCGATGCTGT CATCTATCTC 
AAGGCCCTGT CCACGGACTC GGTGGAGCGC CTGCCTGTCT ACAACAAGTC AGCCTGGCGC 
CACTACCAGA CCATCCAGGA GGCCGGCGAC TGGTGTGTGC CCAGCACGGA GCCCAAGAAC 
ACGTCCACGT ACCGCGTCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002602.2
CDS	86..1309
Translation	MRWVWALLKNASLAGAPKYIEHFSKFSPSPLSMKQFLDFGSSNACEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS

Target ORF information:

RefSeq Version	NM_002611.5
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate dehydrogenase kinase 2 (PDK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002611.5

ORF Insert Sequence:

ATGCGCTGGG TGTGGGCGCT GCTGAAGAAT GCGTCCCTGG CAGGGGCGCC CAAGTACATA 
GAGCACTTCA GCAAGTTCTC CCCGTCCCCG CTGTCCATGA AGCAGTTTCT GGACTTCGGA 
TCCAGCAATG CCTGTGAGAA AACCTCCTTC ACCTTCCTCA GGCAGGAGCT GCCTGTGCGC 
CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCCGACC GAGTGCTGAG CACACCCTCC 
GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGCCTCCTGG ACATCATGGA GTTCCTGGAC 
AAGGATCCCG AGGACCATCG CACCCTGAGC CAGTTCACTG ACGCCCTGGT CACCATCCGG 
AACCGGCACA ACGACGTGGT GCCCACCATG GCACAAGGCG TGCTTGAGTA CAAGGACACC 
TACGGCGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC 
AGCCGCATCT CCATCCGCAT GCTCATCAAC CAGCACACCC TCATCTTTGA TGGCAGCACC 
AACCCAGCCC ATCCCAAACA CATCGGCAGC ATCGACCCCA ACTGCAACGT CTCTGAGGTG 
GTCAAAGATG CCTACGACAT GGCTAAGCTC CTGTGTGACA AGTATTACAT GGCCTCACCT 
GACCTGGAGA TCCAGGAGAT CAATGCAGCC AACTCCAAAC AGCCGATTCA CATGGTCTAC 
GTCCCCTCCC ACCTCTACCA CATGCTCTTT GAGCTCTTCA AGAATGCCAT GAGGGCGACT 
GTGGAAAGCC ATGAGTCCAG CCTCATTCTC CCACCCATCA AGGTCATGGT GGCCTTGGGT 
GAGGAAGATC TGTCCATCAA GATGAGTGAC CGAGGTGGGG GTGTTCCCTT GAGGAAGATT 
GAGCGACTCT TCAGCTACAT GTACTCCACA GCACCCACCC CCCAGCCTGG CACCGGGGGA 
ACGCCGCTGG CTGGCTTTGG TTATGGGCTC CCCATTTCCC GCCTCTACGC CAAGTACTTC 
CAGGGAGACC TGCAGCTCTT CTCCATGGAA GGCTTTGGGA CCGATGCTGT CATCTATCTC 
AAGGCCCTGT CCACGGACTC GGTGGAGCGC CTGCCTGTCT ACAACAAGTC AGCCTGGCGC 
CACTACCAGA CCATCCAGGA GGCCGGCGAC TGGTGTGTGC CCAGCACGGA GCCCAAGAAC 
ACGTCCACGT ACCGCGTCAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MiR-422a regulates cellular metabolism and malignancy by targeting pyruvate dehydrogenase kinase 2 in gastric cancer.
Cell death & disease9(5)505(2018 05)
He Z,Li Z,Zhang X,Yin K,Wang W,Xu Z,Li B,Zhang L,Xu J,Sun G,Wang L,Li Q,Huang X,Zhang L,Zhang D,Xu H,Xu Z

The Mitochondrial Rhomboid Protease PARL Is Regulated by PDK2 to Integrate Mitochondrial Quality Control and Metabolism.
Cell reports18(6)1458-1472(2017 02)
Shi G,McQuibban GA

PDK2 and ABCG2 genes polymorphisms are correlated with blood glucose levels and uric acid in Tibetan gout patients.
Genetics and molecular research : GMR15(1)(2016 Feb)
Ren YC,Jin TB,Sun XD,Geng TT,Zhang MX,Wang L,Feng T,Kang LL,Chen C

Activation of mitochondrial oxidation by PDK2 inhibition reverses cisplatin resistance in head and neck cancer.
Cancer letters371(1)20-9(2016 Feb)
Roh JL,Park JY,Kim EH,Jang HJ,Kwon M

PDK2-mediated alternative splicing switches Bnip3 from cell death to cell survival.
The Journal of cell biology210(7)1101-15(2015 Sep)
Gang H,Dhingra R,Lin J,Hai Y,Aviv Y,Margulets V,Hamedani M,Thanasupawat T,Leygue E,Klonisch T,Davie JR,Kirshenbaum LA

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PDK2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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