NTHL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NTHL1
Entrez Gene ID	4913
Full Name	nth like DNA glycosylase 1
Synonyms	FAP3,NTH1,OCTS3,hNTH1
General protein information	Preferred Names nth like DNA glycosylase 1 Names endonuclease III-like protein 1 DNA glycoslyase/AP lyase DNA glycosylase/AP lyase bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase nth endonuclease III-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008].
Disorder MIM:	602656
Disorder Html:	Familial adenomatous polyposis 3, 616415 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002528.5	NP_002519.1	endonuclease III-like protein 1
NM_001318193.2	NP_001305122.2	endonuclease III-like protein 1 isoform 2
NM_001318193.1	NP_001305122.1	endonuclease III-like protein 1 isoform 2
NM_002528.7	NP_002519.2	endonuclease III-like protein 1 isoform 1
XM_017023253.1	XP_016878742.1	endonuclease III-like protein 1 isoform X1
NM_002528.6	NP_002519.1	endonuclease III-like protein 1 isoform 1
NM_001318194.1	NP_001305123.1	endonuclease III-like protein 1 isoform 3
NM_001318194.2	NP_001305123.1	endonuclease III-like protein 1 isoform 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NTHL1	XP_853674.1
Xenopus tropicalis (tropical clawed frog)	LOC100488928	XP_004918108.1
Magnaporthe oryzae (rice blast fungus)	MGG_06014	XP_003711861.1
Homo sapiens (human)	NTHL1	NP_002519.1
Macaca mulatta (Rhesus monkey)	NTHL1	XP_001082772.1
Drosophila melanogaster (fruit fly)	CG9272	NP_610078.2
Xenopus tropicalis (tropical clawed frog)	nthl1	NP_001037884.1
Bos taurus (cattle)	NTHL1	NP_001039862.1
Rattus norvegicus (Norway rat)	Nthl1	NP_001099198.1
Gallus gallus (chicken)	NTHL1	NP_001073043.1
Danio rerio (zebrafish)	nthl1	XP_001346643.3
Schizosaccharomyces pombe (fission yeast)	nth1	NP_593210.1
Mus musculus (house mouse)	Nthl1	NP_032769.2
Caenorhabditis elegans (roundworm)	nth-1	NP_001254907.1
Neurospora crassa	NCU06654	XP_960699.2

Gene Ontology
Bibliography

Related articles in PubMed

The lyase activity of bifunctional DNA glycosylases and the 3'-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes.
Maher RL, Wallace SS, Pederson DS
Nucleic acids research47(6)2922-2931(2019 04)

Mutational Signature Analysis Reveals NTHL1 Deficiency to Cause a Multi-tumor Phenotype.
Grolleman JE, de Voer RM, Elsayed FA, Nielsen M, Weren RDA, Palles C, Ligtenberg MJL, Vos JR, Ten Broeke SW, de Miranda NFCC, Kuiper RA, Kamping EJ, Jansen EAM, Vink-Börger ME, Popp I, Lang A, Spier I, Hüneburg R, James PA, Li N, Staninova M, Lindsay H, Cockburn D, Spasic-Boskovic O, Clendenning M, Sweet K, Capellá G, Sjursen W, Høberg-Vetti H, Jongmans MC, Neveling K, Geurts van Kessel A, Morreau H, Hes FJ, Sijmons RH, Schackert HK, Ruiz-Ponte C, Dymerska D, Lubinski J, Rivera B, Foulkes WD, Tomlinson IP, Valle L, Buchanan DD, Kenwrick S, Adlard J, Dimovski AJ, Campbell IG, Aretz S, Schindler D, van Wezel T, Hoogerbrugge N, Kuiper RP
Cancer cell35(2)256-266.e5(2019 02)

Defective repair capacity of variant proteins of the DNA glycosylase NTHL1 for 5-hydroxyuracil, an oxidation product of cytosine.
Shinmura K, Kato H, Kawanishi Y, Goto M, Tao H, Yoshimura K, Nakamura S, Misawa K, Sugimura H
Free radical biology & medicine131264-273(2019 02)

Complex interplay of lesion-specific DNA repair enzyme on bistranded clustered DNA damage harboring Tg:G mismatch in nucleosome core particles.
Kumari B, Sinha KK, DAS P
Journal of biosciences43(4)575-583(2018 Sep)

NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress.
Williams SC, Parsons JL
Molecular and cellular biology38(12)(2018 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NTHL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NTHL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu100783	NM_001318194.2 Latest version!	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu107750	NM_002528.7 Latest version!	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu107812	NM_001318193.2 Latest version!	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu59350	NM_001318193.1 Latest version!	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu86047	XM_017023253.1 Latest version!	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu22043	NM_002528.6 Latest version!	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
View Old Accession Versions >>
OHu22043	NM_002528.5	Homo sapiens nth-like DNA glycosylase 1 (NTHL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OHu100783	NM_001318194.1	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NTHL1 ( NM_001318194.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305123.1 Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100783
Clone ID Related Accession (Same CDS sequence)	NM_001318194.1 , NM_001318194.2
Accession Version	NM_001318194.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545408000000
Organism	Homo sapiens(human)
Product	endonuclease III-like protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY094895.1, BG468617.1 and BC003014.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3, which has a distinct N-terminus, and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG469318.1, BG468617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCTC GGACAGTGAG AAAGGTACGC AGGTACCAGG TGCTGCTGTC ACTGATGCTC 
TCCAGCCAAA CCAAAGACCA GGTGACGGCG GGCGCCATGC AGCGACTGCG GGCGCGGGGC 
CTGACGGTGG ACAGCATCCT GCAGACAGAT GATGCCACGC TGGGCAAGCT CATCTACCCC 
GTCGGTTTCT GGAGGAGCAA GGTGAAATAC ATCAAGCAGA CCAGCGCCAT CCTGCAGCAG 
CACTACGGTG GGGACATCCC AGCCTCTGTG GCCGAGCTGG TGGCGCTGCC GGGTGTTGGG 
CCCAAGATGG CACACCTGGC TATGGCTGTG GCCTGGGGCA CTGTGTCAGG CATTGCAGTG 
GACACGCATG TGCACAGAAT CGCCAACAGG CTGAGGTGGA CCAAGAAGGC AACCAAGTCC 
CCAGAGGAGA CCCGCGCCGC CCTGGAGGAG TGGCTGCCTA GGGAGCTGTG GCACGAGATC 
AATGGACTCT TGGTGGGCTT CGGCCAGCAG ACCTGTCTGC CTGTGCACCC TCGCTGCCAC 
GCCTGCCTCA ACCAAGCCCT CTGCCCGGCC GCCCAGGGTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305123.1
CDS	225..809
Translation	MRARTVRKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Target ORF information:

RefSeq Version	NM_001318194.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318194.1

ORF Insert Sequence:

ATGAGGGCTC GGACAGTGAG AAAGGTACGC AGGTACCAGG TGCTGCTGTC ACTGATGCTC 
TCCAGCCAAA CCAAAGACCA GGTGACGGCG GGCGCCATGC AGCGACTGCG GGCGCGGGGC 
CTGACGGTGG ACAGCATCCT GCAGACAGAT GATGCCACGC TGGGCAAGCT CATCTACCCC 
GTCGGTTTCT GGAGGAGCAA GGTGAAATAC ATCAAGCAGA CCAGCGCCAT CCTGCAGCAG 
CACTACGGTG GGGACATCCC AGCCTCTGTG GCCGAGCTGG TGGCGCTGCC GGGTGTTGGG 
CCCAAGATGG CACACCTGGC TATGGCTGTG GCCTGGGGCA CTGTGTCAGG CATTGCAGTG 
GACACGCATG TGCACAGAAT CGCCAACAGG CTGAGGTGGA CCAAGAAGGC AACCAAGTCC 
CCAGAGGAGA CCCGCGCCGC CCTGGAGGAG TGGCTGCCTA GGGAGCTGTG GCACGAGATC 
AATGGACTCT TGGTGGGCTT CGGCCAGCAG ACCTGTCTGC CTGTGCACCC TCGCTGCCAC 
GCCTGCCTCA ACCAAGCCCT CTGCCCGGCC GCCCAGGGTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTHL1 ( NM_001318194.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305123.1 Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100783
Clone ID Related Accession (Same CDS sequence)	NM_001318194.1 , NM_001318194.2
Accession Version	NM_001318194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	endonuclease III-like protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY094895.1, BG468617.1 and BC003014.1. On May 31, 2019 this sequence version replaced NM_001318194.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3, which has a distinct N-terminus, and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG468617.1, BG469318.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGCTC GGACAGTGAG AAAGGTACGC AGGTACCAGG TGCTGCTGTC ACTGATGCTC 
TCCAGCCAAA CCAAAGACCA GGTGACGGCG GGCGCCATGC AGCGACTGCG GGCGCGGGGC 
CTGACGGTGG ACAGCATCCT GCAGACAGAT GATGCCACGC TGGGCAAGCT CATCTACCCC 
GTCGGTTTCT GGAGGAGCAA GGTGAAATAC ATCAAGCAGA CCAGCGCCAT CCTGCAGCAG 
CACTACGGTG GGGACATCCC AGCCTCTGTG GCCGAGCTGG TGGCGCTGCC GGGTGTTGGG 
CCCAAGATGG CACACCTGGC TATGGCTGTG GCCTGGGGCA CTGTGTCAGG CATTGCAGTG 
GACACGCATG TGCACAGAAT CGCCAACAGG CTGAGGTGGA CCAAGAAGGC AACCAAGTCC 
CCAGAGGAGA CCCGCGCCGC CCTGGAGGAG TGGCTGCCTA GGGAGCTGTG GCACGAGATC 
AATGGACTCT TGGTGGGCTT CGGCCAGCAG ACCTGTCTGC CTGTGCACCC TCGCTGCCAC 
GCCTGCCTCA ACCAAGCCCT CTGCCCGGCC GCCCAGGGTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305123.1
CDS	190..774
Translation	MRARTVRKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Target ORF information:

RefSeq Version	NM_001318194.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318194.2

ORF Insert Sequence:

ATGAGGGCTC GGACAGTGAG AAAGGTACGC AGGTACCAGG TGCTGCTGTC ACTGATGCTC 
TCCAGCCAAA CCAAAGACCA GGTGACGGCG GGCGCCATGC AGCGACTGCG GGCGCGGGGC 
CTGACGGTGG ACAGCATCCT GCAGACAGAT GATGCCACGC TGGGCAAGCT CATCTACCCC 
GTCGGTTTCT GGAGGAGCAA GGTGAAATAC ATCAAGCAGA CCAGCGCCAT CCTGCAGCAG 
CACTACGGTG GGGACATCCC AGCCTCTGTG GCCGAGCTGG TGGCGCTGCC GGGTGTTGGG 
CCCAAGATGG CACACCTGGC TATGGCTGTG GCCTGGGGCA CTGTGTCAGG CATTGCAGTG 
GACACGCATG TGCACAGAAT CGCCAACAGG CTGAGGTGGA CCAAGAAGGC AACCAAGTCC 
CCAGAGGAGA CCCGCGCCGC CCTGGAGGAG TGGCTGCCTA GGGAGCTGTG GCACGAGATC 
AATGGACTCT TGGTGGGCTT CGGCCAGCAG ACCTGTCTGC CTGTGCACCC TCGCTGCCAC 
GCCTGCCTCA ACCAAGCCCT CTGCCCGGCC GCCCAGGGTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTHL1 ( NM_002528.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_002519.2 Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107750
Clone ID Related Accession (Same CDS sequence)	NM_002528.7
Accession Version	NM_002528.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Homo sapiens(human)
Product	endonuclease III-like protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U79718.1 and U81285.1. This sequence is a reference standard in the RefSeqGene project. On Nov 15, 2018 this sequence version replaced NM_002528.6. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U81285.1, SRR7410570.571675.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000651570.2/ ENSP00000498421.1 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCGCCT TGAGCGCGAG GATGCTGACC CGGAGCCGGA GCCTGGGACC CGGGGCTGGG 
CCGCGGGGGT GTAGGGAGGA GCCCGGGCCT CTCCGGAGAA GAGAGGCTGC AGCAGAAGCG 
AGGAAAAGCC ACAGCCCCGT GAAGCGTCCG CGGAAAGCAC AGAGACTGCG TGTGGCCTAT 
GAGGGCTCGG ACAGTGAGAA AGGTGAGGGG GCTGAGCCCC TCAAGGTGCC AGTCTGGGAG 
CCCCAGGACT GGCAGCAACA GCTGGTCAAC ATCCGTGCCA TGAGGAACAA AAAGGATGCA 
CCTGTGGACC ATCTGGGGAC TGAGCACTGC TATGACTCCA GTGCCCCCCC AAAGGTACGC 
AGGTACCAGG TGCTGCTGTC ACTGATGCTC TCCAGCCAAA CCAAAGACCA GGTGACGGCG 
GGCGCCATGC AGCGACTGCG GGCGCGGGGC CTGACGGTGG ACAGCATCCT GCAGACAGAT 
GATGCCACGC TGGGCAAGCT CATCTACCCC GTCGGTTTCT GGAGGAGCAA GGTGAAATAC 
ATCAAGCAGA CCAGCGCCAT CCTGCAGCAG CACTACGGTG GGGACATCCC AGCCTCTGTG 
GCCGAGCTGG TGGCGCTGCC GGGTGTTGGG CCCAAGATGG CACACCTGGC TATGGCTGTG 
GCCTGGGGCA CTGTGTCAGG CATTGCAGTG GACACGCATG TGCACAGAAT CGCCAACAGG 
CTGAGGTGGA CCAAGAAGGC AACCAAGTCC CCAGAGGAGA CCCGCGCCGC CCTGGAGGAG 
TGGCTGCCTA GGGAGCTGTG GCACGAGATC AATGGACTCT TGGTGGGCTT CGGCCAGCAG 
ACCTGTCTGC CTGTGCACCC TCGCTGCCAC GCCTGCCTCA ACCAAGCCCT CTGCCCGGCC 
GCCCAGGGTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002519.2
CDS	12..926
Translation	MTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Target ORF information:

RefSeq Version	NM_002528.7
Organism	Homo sapiens(human)
Definition	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002528.7

ORF Insert Sequence:

ATGACCGCCT TGAGCGCGAG GATGCTGACC CGGAGCCGGA GCCTGGGACC CGGGGCTGGG 
CCGCGGGGGT GTAGGGAGGA GCCCGGGCCT CTCCGGAGAA GAGAGGCTGC AGCAGAAGCG 
AGGAAAAGCC ACAGCCCCGT GAAGCGTCCG CGGAAAGCAC AGAGACTGCG TGTGGCCTAT 
GAGGGCTCGG ACAGTGAGAA AGGTGAGGGG GCTGAGCCCC TCAAGGTGCC AGTCTGGGAG 
CCCCAGGACT GGCAGCAACA GCTGGTCAAC ATCCGTGCCA TGAGGAACAA AAAGGATGCA 
CCTGTGGACC ATCTGGGGAC TGAGCACTGC TATGACTCCA GTGCCCCCCC AAAGGTACGC 
AGGTACCAGG TGCTGCTGTC ACTGATGCTC TCCAGCCAAA CCAAAGACCA GGTGACGGCG 
GGCGCCATGC AGCGACTGCG GGCGCGGGGC CTGACGGTGG ACAGCATCCT GCAGACAGAT 
GATGCCACGC TGGGCAAGCT CATCTACCCC GTCGGTTTCT GGAGGAGCAA GGTGAAATAC 
ATCAAGCAGA CCAGCGCCAT CCTGCAGCAG CACTACGGTG GGGACATCCC AGCCTCTGTG 
GCCGAGCTGG TGGCGCTGCC GGGTGTTGGG CCCAAGATGG CACACCTGGC TATGGCTGTG 
GCCTGGGGCA CTGTGTCAGG CATTGCAGTG GACACGCATG TGCACAGAAT CGCCAACAGG 
CTGAGGTGGA CCAAGAAGGC AACCAAGTCC CCAGAGGAGA CCCGCGCCGC CCTGGAGGAG 
TGGCTGCCTA GGGAGCTGTG GCACGAGATC AATGGACTCT TGGTGGGCTT CGGCCAGCAG 
ACCTGTCTGC CTGTGCACCC TCGCTGCCAC GCCTGCCTCA ACCAAGCCCT CTGCCCGGCC 
GCCCAGGGTC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTHL1 ( NM_001318193.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305122.2 Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu107812
Clone ID Related Accession (Same CDS sequence)	NM_001318193.2
Accession Version	NM_001318193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	endonuclease III-like protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U79718.1, BC003014.1 and BE900738.1. On Nov 15, 2018 this sequence version replaced NM_001318193.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.3041.1, BE796807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGCCT TGAGCGCGAG GATGCTGACC CGGAGCCGGA GCCTGGGACC CGGGGCTGGG 
CCGCGGGGGT GTAGGGAGGA GCCCGGGCCT CTCCGGAGAA GAGAGGCTGC AGCAGAAGCG 
AGGAAAAGCC ACAGCCCCGT GAAGCGTCCG CGGAAAGCAC AGAGACTGCG TGTGGCCTAT 
GAGGGCTCGG ACAGTGAGAA AGGTGAGGGG GCTGAGCCCC TCAAGGTGCC AGTCTGGGAG 
CCCCAGGACT GGCAGCAACA GCTGGTCAAC ATCCGTGCCA TGAGGAACAA AAAGGATGCA 
CCTGTGGACC ATCTGGGGAC TGAGCACTGC TATGACTCCA GTGCCCCCCC AAAGAGCAAG 
GTGAAATACA TCAAGCAGAC CAGCGCCATC CTGCAGCAGC ACTACGGTGG GGACATCCCA 
GCCTCTGTGG CCGAGCTGGT GGCGCTGCCG GGTGTTGGGC CCAAGATGGC ACACCTGGCT 
ATGGCTGTGG CCTGGGGCAC TGTGTCAGGC ATTGCAGTGG ACACGCATGT GCACAGAATC 
GCCAACAGGC TGAGGTGGAC CAAGAAGGCA ACCAAGTCCC CAGAGGAGAC CCGCGCCGCC 
CTGGAGGAGT GGCTGCCTAG GGAGCTGTGG CACGAGATCA ATGGACTCTT GGTGGGCTTC 
GGCCAGCAGA CCTGTCTGCC TGTGCACCCT CGCTGCCACG CCTGCCTCAA CCAAGCCCTC 
TGCCCGGCCG CCCAGGGTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305122.2
CDS	12..755
Translation	MTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Target ORF information:

RefSeq Version	NM_001318193.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318193.2

ORF Insert Sequence:

ATGACCGCCT TGAGCGCGAG GATGCTGACC CGGAGCCGGA GCCTGGGACC CGGGGCTGGG 
CCGCGGGGGT GTAGGGAGGA GCCCGGGCCT CTCCGGAGAA GAGAGGCTGC AGCAGAAGCG 
AGGAAAAGCC ACAGCCCCGT GAAGCGTCCG CGGAAAGCAC AGAGACTGCG TGTGGCCTAT 
GAGGGCTCGG ACAGTGAGAA AGGTGAGGGG GCTGAGCCCC TCAAGGTGCC AGTCTGGGAG 
CCCCAGGACT GGCAGCAACA GCTGGTCAAC ATCCGTGCCA TGAGGAACAA AAAGGATGCA 
CCTGTGGACC ATCTGGGGAC TGAGCACTGC TATGACTCCA GTGCCCCCCC AAAGAGCAAG 
GTGAAATACA TCAAGCAGAC CAGCGCCATC CTGCAGCAGC ACTACGGTGG GGACATCCCA 
GCCTCTGTGG CCGAGCTGGT GGCGCTGCCG GGTGTTGGGC CCAAGATGGC ACACCTGGCT 
ATGGCTGTGG CCTGGGGCAC TGTGTCAGGC ATTGCAGTGG ACACGCATGT GCACAGAATC 
GCCAACAGGC TGAGGTGGAC CAAGAAGGCA ACCAAGTCCC CAGAGGAGAC CCGCGCCGCC 
CTGGAGGAGT GGCTGCCTAG GGAGCTGTGG CACGAGATCA ATGGACTCTT GGTGGGCTTC 
GGCCAGCAGA CCTGTCTGCC TGTGCACCCT CGCTGCCACG CCTGCCTCAA CCAAGCCCTC 
TGCCCGGCCG CCCAGGGTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTHL1 ( NM_001318193.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305122.1 Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu59350
Clone ID Related Accession (Same CDS sequence)	NM_001318193.1
Accession Version	NM_001318193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514649600000
Organism	Homo sapiens(human)
Product	endonuclease III-like protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY094895.1, BE900738.1 and BC003014.1. On Dec 29, 2015 this sequence version replaced XM_011522505.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.3041.1, BE796807.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGAG CAAGGTGAAA TACATCAAGC AGACCAGCGC CATCCTGCAG 
CAGCACTACG GTGGGGACAT CCCAGCCTCT GTGGCCGAGC TGGTGGCGCT GCCGGGTGTT 
GGGCCCAAGA TGGCACACCT GGCTATGGCT GTGGCCTGGG GCACTGTGTC AGGCATTGCA 
GTGGACACGC ATGTGCACAG AATCGCCAAC AGGCTGAGGT GGACCAAGAA GGCAACCAAG 
TCCCCAGAGG AGACCCGCGC CGCCCTGGAG GAGTGGCTGC CTAGGGAGCT GTGGCACGAG 
ATCAATGGAC TCTTGGTGGG CTTCGGCCAG CAGACCTGTC TGCCTGTGCA CCCTCGCTGC 
CACGCCTGCC TCAACCAAGC CCTCTGCCCG GCCGCCCAGG GTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305122.1
CDS	23..790
Translation	MCSPQESGMTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Target ORF information:

RefSeq Version	NM_001318193.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318193.1

ORF Insert Sequence:

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGAG CAAGGTGAAA TACATCAAGC AGACCAGCGC CATCCTGCAG 
CAGCACTACG GTGGGGACAT CCCAGCCTCT GTGGCCGAGC TGGTGGCGCT GCCGGGTGTT 
GGGCCCAAGA TGGCACACCT GGCTATGGCT GTGGCCTGGG GCACTGTGTC AGGCATTGCA 
GTGGACACGC ATGTGCACAG AATCGCCAAC AGGCTGAGGT GGACCAAGAA GGCAACCAAG 
TCCCCAGAGG AGACCCGCGC CGCCCTGGAG GAGTGGCTGC CTAGGGAGCT GTGGCACGAG 
ATCAATGGAC TCTTGGTGGG CTTCGGCCAG CAGACCTGTC TGCCTGTGCA CCCTCGCTGC 
CACGCCTGCC TCAACCAAGC CCTCTGCCCG GCCGCCCAGG GTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTHL1 ( XM_017023253.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878742.1 Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86047
Clone ID Related Accession (Same CDS sequence)	XM_017023253.1
Accession Version	XM_017023253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	endonuclease III-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGGT ACGCAGGTAC CAGGTGCTGC TGTCACTGAT GCTCTCCAGC 
CAAACCAAAG ACCAGGTGAC GGCGGGCGCC ATGCAGCGAC TGCGGGCGCG GGGCCTGACG 
GTGGACAGCA TCCTGCAGAC AGATGATGCC ACGCTGGGCA AGCTCATCTA CCCCGTCGGT 
TTCTGGAGGA GCAAGGTGAA ATACATCAAG CAGACCAGCG CCATCCTGCA GCAGCACTAC 
GGTGGGGACA TCCCAGCCTC TGTGGCCGAG CTGGTGGCGC TGCCGGGTGT TGGGCCCAAG 
ATGGCACACC TGGCTATGGC TGTGGCCTGG GGCACTGTGT CAGGCATTGC AGTGGACACG 
CATGTGCACA GAATCGCCAA CAGGCTGAGG TGGACCAAGA AGGCAACCAA GTCCCCAGAG 
GAGACCCGCG CCGCCCTGGA GGAGTGGCTG CCTAGGTATG AGTATGTGGG GGGCTTCCTA 
GGGAGAAGAG CTCACCCCGC CCGCCCTCTG TCAGCACTGA CCCAGCCCCC ACCCCCACAG 
GGAGCTGTGG CACGAGATCA ATGGACTCTT GGTGGGCTTC GGCCAGCAGA CCTGTCTGCC 
TGTGCACCCT CGCTGCCACG CCTGCCTCAA CCAAGCCCTC TGCCCGGCCG CCCAGGGTCT 
CTGATGGCCG CATGGCTCTG GCCGAGGTGC CGCTGTGGCC ACCGTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878742.1
CDS	26..1096
Translation	MCSPQESGMTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRYEYVGGFLGRRAHPARPLSALTQPPPPQGAVARDQWTLGGLRPADLSACAPSLPRLPQPSPLPGRPGSLMAAWLWPRCRCGHRL

Target ORF information:

RefSeq Version	XM_017023253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023253.1

ORF Insert Sequence:

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGGT ACGCAGGTAC CAGGTGCTGC TGTCACTGAT GCTCTCCAGC 
CAAACCAAAG ACCAGGTGAC GGCGGGCGCC ATGCAGCGAC TGCGGGCGCG GGGCCTGACG 
GTGGACAGCA TCCTGCAGAC AGATGATGCC ACGCTGGGCA AGCTCATCTA CCCCGTCGGT 
TTCTGGAGGA GCAAGGTGAA ATACATCAAG CAGACCAGCG CCATCCTGCA GCAGCACTAC 
GGTGGGGACA TCCCAGCCTC TGTGGCCGAG CTGGTGGCGC TGCCGGGTGT TGGGCCCAAG 
ATGGCACACC TGGCTATGGC TGTGGCCTGG GGCACTGTGT CAGGCATTGC AGTGGACACG 
CATGTGCACA GAATCGCCAA CAGGCTGAGG TGGACCAAGA AGGCAACCAA GTCCCCAGAG 
GAGACCCGCG CCGCCCTGGA GGAGTGGCTG CCTAGGTATG AGTATGTGGG GGGCTTCCTA 
GGGAGAAGAG CTCACCCCGC CCGCCCTCTG TCAGCACTGA CCCAGCCCCC ACCCCCACAG 
GGAGCTGTGG CACGAGATCA ATGGACTCTT GGTGGGCTTC GGCCAGCAGA CCTGTCTGCC 
TGTGCACCCT CGCTGCCACG CCTGCCTCAA CCAAGCCCTC TGCCCGGCCG CCCAGGGTCT 
CTGATGGCCG CATGGCTCTG GCCGAGGTGC CGCTGTGGCC ACCGTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTHL1 ( NM_002528.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002519.1 Homo sapiens nth-like DNA glycosylase 1 (NTHL1), mRNA.

Price & Availability↑

CloneID	OHu22043
Clone ID Related Accession (Same CDS sequence)	NM_002528.5 , NM_002528.6
Accession Version	NM_002528.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1435075200000
Organism	Homo sapiens(Human)
Product	endonuclease III-like protein 1
Comment

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGGT ACGCAGGTAC CAGGTGCTGC TGTCACTGAT GCTCTCCAGC 
CAAACCAAAG ACCAGGTGAC GGCGGGCGCC ATGCAGCGAC TGCGGGCGCG GGGCCTGACG 
GTGGACAGCA TCCTGCAGAC AGATGATGCC ACGCTGGGCA AGCTCATCTA CCCCGTCGGT 
TTCTGGAGGA GCAAGGTGAA ATACATCAAG CAGACCAGCG CCATCCTGCA GCAGCACTAC 
GGTGGGGACA TCCCAGCCTC TGTGGCCGAG CTGGTGGCGC TGCCGGGTGT TGGGCCCAAG 
ATGGCACACC TGGCTATGGC TGTGGCCTGG GGCACTGTGT CAGGCATTGC AGTGGACACG 
CATGTGCACA GAATCGCCAA CAGGCTGAGG TGGACCAAGA AGGCAACCAA GTCCCCAGAG 
GAGACCCGCG CCGCCCTGGA GGAGTGGCTG CCTAGGGAGC TGTGGCACGA GATCAATGGA 
CTCTTGGTGG GCTTCGGCCA GCAGACCTGT CTGCCTGTGC ACCCTCGCTG CCACGCCTGC 
CTCAACCAAG CCCTCTGCCC GGCCGCCCAG GGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002519.1
Translation	MCSPQESGMTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Target ORF information:

RefSeq Version	NM_002528.5
Organism	Homo sapiens(Human)
Definition	Homo sapiens nth-like DNA glycosylase 1 (NTHL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002528.5

ORF Insert Sequence:

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGGT ACGCAGGTAC CAGGTGCTGC TGTCACTGAT GCTCTCCAGC 
CAAACCAAAG ACCAGGTGAC GGCGGGCGCC ATGCAGCGAC TGCGGGCGCG GGGCCTGACG 
GTGGACAGCA TCCTGCAGAC AGATGATGCC ACGCTGGGCA AGCTCATCTA CCCCGTCGGT 
TTCTGGAGGA GCAAGGTGAA ATACATCAAG CAGACCAGCG CCATCCTGCA GCAGCACTAC 
GGTGGGGACA TCCCAGCCTC TGTGGCCGAG CTGGTGGCGC TGCCGGGTGT TGGGCCCAAG 
ATGGCACACC TGGCTATGGC TGTGGCCTGG GGCACTGTGT CAGGCATTGC AGTGGACACG 
CATGTGCACA GAATCGCCAA CAGGCTGAGG TGGACCAAGA AGGCAACCAA GTCCCCAGAG 
GAGACCCGCG CCGCCCTGGA GGAGTGGCTG CCTAGGGAGC TGTGGCACGA GATCAATGGA 
CTCTTGGTGG GCTTCGGCCA GCAGACCTGT CTGCCTGTGC ACCCTCGCTG CCACGCCTGC 
CTCAACCAAG CCCTCTGCCC GGCCGCCCAG GGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NTHL1 ( NM_002528.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002519.1 Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22043
Clone ID Related Accession (Same CDS sequence)	NM_002528.5 , NM_002528.6
Accession Version	NM_002528.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514649600000
Organism	Homo sapiens(human)
Product	endonuclease III-like protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY094895.1, HY057336.1, U79718.1 and BC003014.1. This sequence is a reference standard in the RefSeqGene project. On Dec 29, 2015 this sequence version replaced NM_002528.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003014.1, SRR1163655.254853.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGGT ACGCAGGTAC CAGGTGCTGC TGTCACTGAT GCTCTCCAGC 
CAAACCAAAG ACCAGGTGAC GGCGGGCGCC ATGCAGCGAC TGCGGGCGCG GGGCCTGACG 
GTGGACAGCA TCCTGCAGAC AGATGATGCC ACGCTGGGCA AGCTCATCTA CCCCGTCGGT 
TTCTGGAGGA GCAAGGTGAA ATACATCAAG CAGACCAGCG CCATCCTGCA GCAGCACTAC 
GGTGGGGACA TCCCAGCCTC TGTGGCCGAG CTGGTGGCGC TGCCGGGTGT TGGGCCCAAG 
ATGGCACACC TGGCTATGGC TGTGGCCTGG GGCACTGTGT CAGGCATTGC AGTGGACACG 
CATGTGCACA GAATCGCCAA CAGGCTGAGG TGGACCAAGA AGGCAACCAA GTCCCCAGAG 
GAGACCCGCG CCGCCCTGGA GGAGTGGCTG CCTAGGGAGC TGTGGCACGA GATCAATGGA 
CTCTTGGTGG GCTTCGGCCA GCAGACCTGT CTGCCTGTGC ACCCTCGCTG CCACGCCTGC 
CTCAACCAAG CCCTCTGCCC GGCCGCCCAG GGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002519.1
CDS	23..961
Translation	MCSPQESGMTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Target ORF information:

RefSeq Version	NM_002528.6
Organism	Homo sapiens(human)
Definition	Homo sapiens nth like DNA glycosylase 1 (NTHL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002528.6

ORF Insert Sequence:

ATGTGTAGTC CGCAGGAGTC CGGCATGACC GCCTTGAGCG CGAGGATGCT GACCCGGAGC 
CGGAGCCTGG GACCCGGGGC TGGGCCGCGG GGGTGTAGGG AGGAGCCCGG GCCTCTCCGG 
AGAAGAGAGG CTGCAGCAGA AGCGAGGAAA AGCCACAGCC CCGTGAAGCG TCCGCGGAAA 
GCACAGAGAC TGCGTGTGGC CTATGAGGGC TCGGACAGTG AGAAAGGTGA GGGGGCTGAG 
CCCCTCAAGG TGCCAGTCTG GGAGCCCCAG GACTGGCAGC AACAGCTGGT CAACATCCGT 
GCCATGAGGA ACAAAAAGGA TGCACCTGTG GACCATCTGG GGACTGAGCA CTGCTATGAC 
TCCAGTGCCC CCCCAAAGGT ACGCAGGTAC CAGGTGCTGC TGTCACTGAT GCTCTCCAGC 
CAAACCAAAG ACCAGGTGAC GGCGGGCGCC ATGCAGCGAC TGCGGGCGCG GGGCCTGACG 
GTGGACAGCA TCCTGCAGAC AGATGATGCC ACGCTGGGCA AGCTCATCTA CCCCGTCGGT 
TTCTGGAGGA GCAAGGTGAA ATACATCAAG CAGACCAGCG CCATCCTGCA GCAGCACTAC 
GGTGGGGACA TCCCAGCCTC TGTGGCCGAG CTGGTGGCGC TGCCGGGTGT TGGGCCCAAG 
ATGGCACACC TGGCTATGGC TGTGGCCTGG GGCACTGTGT CAGGCATTGC AGTGGACACG 
CATGTGCACA GAATCGCCAA CAGGCTGAGG TGGACCAAGA AGGCAACCAA GTCCCCAGAG 
GAGACCCGCG CCGCCCTGGA GGAGTGGCTG CCTAGGGAGC TGTGGCACGA GATCAATGGA 
CTCTTGGTGG GCTTCGGCCA GCAGACCTGT CTGCCTGTGC ACCCTCGCTG CCACGCCTGC 
CTCAACCAAG CCCTCTGCCC GGCCGCCCAG GGTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The lyase activity of bifunctional DNA glycosylases and the 3'-diesterase activity of APE1 contribute to the repair of oxidized bases in nucleosomes.
Nucleic acids research47(6)2922-2931(2019 04)
Maher RL,Wallace SS,Pederson DS

Mutational Signature Analysis Reveals NTHL1 Deficiency to Cause a Multi-tumor Phenotype.
Cancer cell35(2)256-266.e5(2019 02)
Grolleman JE,de Voer RM,Elsayed FA,Nielsen M,Weren RDA,Palles C,Ligtenberg MJL,Vos JR,Ten Broeke SW,de Miranda NFCC,Kuiper RA,Kamping EJ,Jansen EAM,Vink-Börger ME,Popp I,Lang A,Spier I,Hüneburg R,James PA,Li N,Staninova M,Lindsay H,Cockburn D,Spasic-Boskovic O,Clendenning M,Sweet K,Capellá G,Sjursen W,Høberg-Vetti H,Jongmans MC,Neveling K,Geurts van Kessel A,Morreau H,Hes FJ,Sijmons RH,Schackert HK,Ruiz-Ponte C,Dymerska D,Lubinski J,Rivera B,Foulkes WD,Tomlinson IP,Valle L,Buchanan DD,Kenwrick S,Adlard J,Dimovski AJ,Campbell IG,Aretz S,Schindler D,van Wezel T,Hoogerbrugge N,Kuiper RP

Defective repair capacity of variant proteins of the DNA glycosylase NTHL1 for 5-hydroxyuracil, an oxidation product of cytosine.
Free radical biology & medicine131264-273(2019 02)
Shinmura K,Kato H,Kawanishi Y,Goto M,Tao H,Yoshimura K,Nakamura S,Misawa K,Sugimura H

Complex interplay of lesion-specific DNA repair enzyme on bistranded clustered DNA damage harboring Tg:G mismatch in nucleosome core particles.
Journal of biosciences43(4)575-583(2018 Sep)
Kumari B,Sinha KK,DAS P

NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress.
Molecular and cellular biology38(12)(2018 06)
Williams SC,Parsons JL

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

NTHL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM