RAB8A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAB8A
Entrez Gene ID	4218
Full Name	RAB8A, member RAS oncogene family
Synonyms	MEL,RAB8
General protein information	Preferred Names RAB8A, member RAS oncogene family Names ras-related protein Rab-8A mel transforming oncogene (RAB8 homolog) mel transforming oncogene (derived from cell line NK14) mel transforming oncogene (derived from cell line NK14)- RAB8 homolog oncogene c-mel ras-associated protein RAB8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11
Summary	The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008].
Disorder MIM:	165040

mRNA and Protein(s)

mRNA	Protein	Name
XM_011528020.2	XP_011526322.1	ras-related protein Rab-8A isoform X1
NM_005370.4	NP_005361.2	ras-related protein Rab-8A
NM_005370.5	NP_005361.2	ras-related protein Rab-8A
XM_011528020.3	XP_011526322.1	ras-related protein Rab-8A isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001211	XP_321946.4
Schizosaccharomyces pombe (fission yeast)	ypt2	NP_594580.1
Eremothecium gossypii	AGOS_AGL021W	NP_986645.1
Neurospora crassa	NCU06404	XP_962242.1
Danio rerio (zebrafish)	rab8a	NP_001083031.1
Arabidopsis thaliana (thale cress)	RAB8	NP_190929.1
Magnaporthe oryzae (rice blast fungus)	MGG_06135	XP_003712007.1
Oryza sativa (rice)	Os07g0239400	NP_001059259.1
Kluyveromyces lactis	KLLA0E12079g	XP_454494.1
Oryza sativa (rice)	Os05g0461300	NP_001055761.1
Canis lupus familiaris (dog)	RAB8A	NP_001003152.1
Gallus gallus (chicken)	RAB8A	NP_001026675.1
Drosophila melanogaster (fruit fly)	Rab8	NP_001246837.1
Saccharomyces cerevisiae (baker's yeast)	SEC4	NP_116650.1
Bos taurus (cattle)	RAB8A	NP_001098951.1
Xenopus tropicalis (tropical clawed frog)	rab8a	XP_002934841.1
Homo sapiens (human)	RAB8A	NP_005361.2
Pan troglodytes (chimpanzee)	RAB8A	NP_001229300.1
Rattus norvegicus (Norway rat)	Rab8a	NP_446450.2
Caenorhabditis elegans (roundworm)	rab-8	NP_491199.2
Arabidopsis thaliana (thale cress)	AT5G59840	NP_200792.1
Oryza sativa (rice)	Os03g0819900	NP_001051716.1
Mus musculus (house mouse)	Rab8a	NP_075615.2
Arabidopsis thaliana (thale cress)	RAB8A	NP_001118780.1

Gene Ontology
Bibliography

Related articles in PubMed

The G2019S variant of leucine-rich repeat kinase 2 (LRRK2) alters endolysosomal trafficking by impairing the function of the GTPase RAB8A.
Rivero-Ríos P, Romo-Lozano M, Madero-Pérez J, Thomas AP, Biosa A, Greggio E, Hilfiker S
The Journal of biological chemistry294(13)4738-4758(2019 03)

Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.
Kim MJ, Jeon S, Burbulla LF, Krainc D
Human molecular genetics27(11)1972-1988(2018 06)

LRRK2 mediated Rab8a phosphorylation promotes lipid storage.
Yu M, Arshad M, Wang W, Zhao D, Xu L, Zhou L
Lipids in health and disease17(1)34(2018 Feb)

Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Madero-Pérez J, Fdez E, Fernández B, Lara Ordóñez AJ, Blanca Ramírez M, Gómez-Suaga P, Waschbüsch D, Lobbestael E, Baekelandt V, Nairn AC, Ruiz-Martínez J, Aiastui A, López de Munain A, Lis P, Comptdaer T, Taymans JM, Chartier-Harlin MC, Beilina A, Gonnelli A, Cookson MR, Greggio E, Hilfiker S
Molecular neurodegeneration13(1)3(2018 01)

Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis.
Steger M, Diez F, Dhekne HS, Lis P, Nirujogi RS, Karayel O, Tonelli F, Martinez TN, Lorentzen E, Pfeffer SR, Alessi DR, Mann M
eLife6(2017 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RAB8A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAB8A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26799	NM_005370.5 Latest version!	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu58939	XM_011528020.3 Latest version!	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu26799	NM_005370.4	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu58939	XM_011528020.2	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAB8A ( NM_005370.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005361.2 Homo sapiens RAB8A, member RAS oncogene family (RAB8A), mRNA.

Price & Availability↑

CloneID	OHu26799
Clone ID Related Accession (Same CDS sequence)	NM_005370.4 , NM_005370.5
Accession Version	NM_005370.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506700800000
Organism	Homo sapiens(human)
Product	ras-related protein Rab-8A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK025165.1, BC002977.1 and BU940750.1. On Jan 2, 2004 this sequence version replaced NM_005370.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.173894.1, AK025165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGAAGGCAAC 
AGCCCCCAGG GGAGCAACCA GGGAGTCAAA ATCACACCGG ACCAGCAGAA GAGGAGCAGC 
TTTTTCCGAT GTGTTCTTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005361.2
CDS	223..846
Translation	MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL

Target ORF information:

RefSeq Version	NM_005370.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005370.4

ORF Insert Sequence:

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGAAGGCAAC 
AGCCCCCAGG GGAGCAACCA GGGAGTCAAA ATCACACCGG ACCAGCAGAA GAGGAGCAGC 
TTTTTCCGAT GTGTTCTTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAB8A ( NM_005370.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005361.2 Homo sapiens RAB8A, member RAS oncogene family (RAB8A), mRNA.

Price & Availability↑

CloneID	OHu26799
Clone ID Related Accession (Same CDS sequence)	NM_005370.4 , NM_005370.5
Accession Version	NM_005370.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	ras-related protein Rab-8A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK025165.1, BC002977.1, BU940750.1 and AC008894.9. On Nov 22, 2018 this sequence version replaced NM_005370.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.28557.1, AK025165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000300935.8/ ENSP00000300935.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGAAGGCAAC 
AGCCCCCAGG GGAGCAACCA GGGAGTCAAA ATCACACCGG ACCAGCAGAA GAGGAGCAGC 
TTTTTCCGAT GTGTTCTTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005361.2
CDS	14..637
Translation	MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL

Target ORF information:

RefSeq Version	NM_005370.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005370.5

ORF Insert Sequence:

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGAAGGCAAC 
AGCCCCCAGG GGAGCAACCA GGGAGTCAAA ATCACACCGG ACCAGCAGAA GAGGAGCAGC 
TTTTTCCGAT GTGTTCTTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAB8A ( XM_011528020.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526322.1 Homo sapiens RAB8A, member RAS oncogene family (RAB8A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58939
Clone ID Related Accession (Same CDS sequence)	XM_011528020.2 , XM_011528020.3
Accession Version	XM_011528020.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	ras-related protein Rab-8A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528020.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGGCCAGAAA 
GACAACATGG TGTGCAAGGA CAGAGAGCCA TGGCTCGGAG AAAAGCTTCC TAGGTCGCTT 
GGGCAGGGCC AGGGCTGGTG TCTGATAACT GCTGGCTCAT CGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526322.1
CDS	11..658
Translation	MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLGQKDNMVCKDREPWLGEKLPRSLGQGQGWCLITAGSSS

Target ORF information:

RefSeq Version	XM_011528020.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528020.2

ORF Insert Sequence:

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGGCCAGAAA 
GACAACATGG TGTGCAAGGA CAGAGAGCCA TGGCTCGGAG AAAAGCTTCC TAGGTCGCTT 
GGGCAGGGCC AGGGCTGGTG TCTGATAACT GCTGGCTCAT CGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAB8A ( XM_011528020.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526322.1 Homo sapiens RAB8A, member RAS oncogene family (RAB8A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58939
Clone ID Related Accession (Same CDS sequence)	XM_011528020.2 , XM_011528020.3
Accession Version	XM_011528020.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	ras-related protein Rab-8A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528020.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGGCCAGAAA 
GACAACATGG TGTGCAAGGA CAGAGAGCCA TGGCTCGGAG AAAAGCTTCC TAGGTCGCTT 
GGGCAGGGCC AGGGCTGGTG TCTGATAACT GCTGGCTCAT CGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526322.1
CDS	27..674
Translation	MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLGQKDNMVCKDREPWLGEKLPRSLGQGQGWCLITAGSSS

Target ORF information:

RefSeq Version	XM_011528020.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAB8A, member RAS oncogene family (RAB8A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528020.3

ORF Insert Sequence:

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTGGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ACTCCACTTT TATCTCCACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGAAT TAAACTGCAG 
ATATGGGACA CAGCCGGTCA GGAACGGTTT CGGACGATCA CAACGGCCTA CTACAGGGGT 
GCAATGGGCA TCATGCTGGT CTACGACATC ACCAACGAGA AGTCCTTCGA CAACATCCGG 
AACTGGATTC GCAACATTGA GGAGCACGCC TCTGCAGACG TCGAAAAGAT GATACTCGGG 
AACAAGTGTG ATGTGAATGA CAAGAGACAA GTTTCCAAGG AACGGGGAGA AAAGCTGGCC 
CTCGACTATG GAATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAAAAT 
GCATTTTTCA CTCTCGCCAG AGATATCAAA GCAAAAATGG ACAAAAAATT GGGCCAGAAA 
GACAACATGG TGTGCAAGGA CAGAGAGCCA TGGCTCGGAG AAAAGCTTCC TAGGTCGCTT 
GGGCAGGGCC AGGGCTGGTG TCTGATAACT GCTGGCTCAT CGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The G2019S variant of leucine-rich repeat kinase 2 (LRRK2) alters endolysosomal trafficking by impairing the function of the GTPase RAB8A.
The Journal of biological chemistry294(13)4738-4758(2019 03)
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Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.
Human molecular genetics27(11)1972-1988(2018 06)
Kim MJ,Jeon S,Burbulla LF,Krainc D

LRRK2 mediated Rab8a phosphorylation promotes lipid storage.
Lipids in health and disease17(1)34(2018 Feb)
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Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.
Molecular neurodegeneration13(1)3(2018 01)
Madero-Pérez J,Fdez E,Fernández B,Lara Ordóñez AJ,Blanca Ramírez M,Gómez-Suaga P,Waschbüsch D,Lobbestael E,Baekelandt V,Nairn AC,Ruiz-Martínez J,Aiastui A,López de Munain A,Lis P,Comptdaer T,Taymans JM,Chartier-Harlin MC,Beilina A,Gonnelli A,Cookson MR,Greggio E,Hilfiker S

Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis.
eLife6(2017 11)
Steger M,Diez F,Dhekne HS,Lis P,Nirujogi RS,Karayel O,Tonelli F,Martinez TN,Lorentzen E,Pfeffer SR,Alessi DR,Mann M

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RAB8A cDNA ORF clone, Homo sapiens(human)

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