ANKHD1-EIF4EBP3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKHD1-EIF4EBP3
Entrez Gene ID	404734
Full Name	ANKHD1-EIF4EBP3 readthrough
Synonyms	MASK-BP3,MASK-BP3ARF
General protein information	Preferred Names ANKHD1-EIF4EBP3 readthrough Names ANKHD1-EIF4EBP3 protein MASK-4E-BP3 alternate reading frame
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	The ANKHD1-EIF4EBP3 mRNA is an infrequent but naturally occurring readthrough transcript of the neighboring ANKHD1 and EIF4EBP3 genes. This readthrough transcript encodes a protein composed mostly of the multiple ankyrin repeats, single KH-domain protein, with its C-terminus encoded in a different reading frame from the shared portion of the EIF4EBP3 gene. The significance of this readthrough mRNA and the function of its protein product have not yet been determined. [provided by RefSeq, Nov 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_020690.5	NP_065741.3	ANKHD1-EIF4EBP3 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANKHD1-EIF4EBP3	NP_065741.3
Canis lupus familiaris (dog)	ANKHD1	NP_001191024.1
Mus musculus (house mouse)	Ankhd1	NP_780584.2
Rattus norvegicus (Norway rat)	Ankhd1	NP_001190982.1
Homo sapiens (human)	ANKHD1	NP_060217.1
Xenopus tropicalis (tropical clawed frog)	ankhd1	NP_001191021.1
Bos taurus (cattle)	ANKHD1	XP_005209554.1
Danio rerio (zebrafish)	ankhd1	NP_001186697.1
Macaca mulatta (Rhesus monkey)	ANKHD1	XP_002804594.1
Gallus gallus (chicken)	ANKHD1	NP_001191026.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of conjoined genes: another mechanism for gene regulation in eukaryotes.
Prakash T, Sharma VK, Adati N, Ozawa R, Kumar N, Nishida Y, Fujikake T, Takeda T, Taylor TD
PloS one5(10)e13284(2010 Oct)

Characterisation of a new splice variant of MASK-BP3(ARF) and MASK human genes, and their expression patterns during haematopoietic cell differentiation.
Santos Duarte Ada S, Traina F, Favaro PM, Bassères DS, de Carvalho IC, Medina Sde S, Costa FF, Saad ST
Gene363113-22(2005 Dec)

Gene fusion and overlapping reading frames in the mammalian genes for 4E-BP3 and MASK.
Poulin F, Brueschke A, Sonenberg N
The Journal of biological chemistry278(52)52290-7(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ANKHD1-EIF4EBP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKHD1-EIF4EBP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OHu00762	NM_020690.5 Latest version!	Homo sapiens ANKHD1-EIF4EBP3 readthrough (ANKHD1-EIF4EBP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKHD1-EIF4EBP3 ( NM_020690.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065741.3 Homo sapiens ANKHD1-EIF4EBP3 readthrough (ANKHD1-EIF4EBP3), mRNA.

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CloneID	OHu00762
Clone ID Related Accession (Same CDS sequence)	NM_020690.5
Accession Version	NM_020690.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525968000000
Organism	Homo sapiens(human)
Product	ANKHD1-EIF4EBP3 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008438.6, AF521883.1 and AC011399.6. On Oct 1, 2010 this sequence version replaced NM_020690.4. ##Evidence-Data-START## Transcript exon combination :: AF521883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 8637, 54882 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGAACATGA ATCTGAAGGT 
GGAAGAACAC CTTTGATGAA AGCTGCAAGA GCTGGTCATT TGTGCACTGT GCAGTTTCTT 
ATTAGCAAAG GTGCCAATGT TAACAGGGCT ACAGCCAATA ATGATCATAC AGTAGTGTCG 
CTGGCATGTG CAGGAGGCCA CCTGGCAGTT GTTGAGCTTC TCTTGGCTCA TGGGGCTGAC 
CCTACTCATC GACTCAAGGA TGGTTCAACA ATGCTCATTG AAGCTGCAAA GGGTGGCCAT 
ACTAATGTAG TTTCTTATCT GTTGGATTAT CCAAATAATG TTCTGTCAGT TCCCACCACA 
GATGTGTCTC AGCTCCCTCC ACCTTCTCAA GATCAGTCTC AGGTGCCACG TGTGCCAACG 
CATACACTTG CCATGGTTGT ACCTCCCCAG GAACCTGACA GAACTTCACA GGAGAACTCT 
CCTGCCCTTT TAGGAGTGCA AAAAGGTACA TCCAAGCAGA AGTCCAGTTC CCTCCAGGTA 
GCAGATCAGG ACCTACTGCC ATCTTTTCAC CCATACCAGC CTTTGGAGTG CATAGTAGAG 
GAGACTGAAG GCAAGCTGAA TGAACTGGGA CAAAGAATTA GTGCTATTGA AAAAGCACAG 
CTTAAGTCAC TGGAGTTAAT TCAAGGTGAA CCTCTGAACA AAGATAAGAT AGAAGAACTT 
AAAAAGAACA GAGAAGAGCA AGTCCAGAAG AAGAAGAAAA TATTGAAAGA ACTGCAGAAA 
GTGGAAAGGC AGTTGCAGAT GAAAACACAG CAGCAATTTA CCAAAGAATA CTTGGAAACC 
AAAGGTCAGA AAGACACAGT GTCTCTACAC CAACAGTGCT CTCATAGAGG AGTCTTCCCA 
GAAGGGGAAG GAGATGGTAG TCTCCCAGAG GATCACTTTT CAGAGTTACC TCAGGTTGAC 
ACAATCTTAT TTAAAGATAA TGATGTTGAT GATGAGCAAC AGTCTCCACC ATCGGCAGAA 
CAGATTGATT TTGTCCCAGT CCAGCCTTTA TCATCTCCAC AGTGTAACTT TTCCAGTGAC 
TTAGGTTCTA ATGGGACAAA TTCTCTTGAA CTTCAGAAAG TATCAGGTAA TCAGCAGATT 
GTAGGACAGC CTCAGATTGC TATTACTGGA CATGATCAGG GGCTGTTAGT TCAAGAACCA 
GATGGACTAA TGGTTGCAAC TCCAGCTCAG ACGCTTACCG ACACTCTTGA TGACCTGATA 
GCAGCTGTGA GTACCAGAGT GCCCACTGGT TCCAACAGTT CTTCTCAGAC CACAGAGTGT 
CTTACACCTG AATCCTGTTC GCAGACTACA AGCAATGTGG CTTCCCAATC GATGCCTCCT 
GTGTATCCTT CAGTTGACAT TGATGCACAT ACTGAGAGCA ATCATGACAC AGCATTAACA 
CTAGCTTGTG CAGGTGGTCA TGAAGAACTT GTATCTGTGC TCATTGCACG GGATGCCAAA 
ATTGAACACA GAGACAAAAA AGGTTTCACA CCACTAATCC TGGCAGCAAC AGCAGGGCAT 
GTTGGAGTTG TTGAAATCCT TTTGGATAAA GGTGGAGATA TAGAAGCACA GTCTGAACGA 
ACTAAGGATA CTCCGCTTTC ATTGGCATGT TCTGGTGGAC GTCAGGAGGT GGTAGACTTG 
CTGCTGGCTC GAGGTGCAAA TAAAGAACAT AGGAACGTAT CTGATTATAC ACCACTGAGT 
CTAGCTGCGT CTGGAGGATA TGTTAATATC ATTAAGATTC TGCTTAATGC TGGGGCAGAA 
ATTAATTCAA GGACTGGGAG TAAACTAGGT ATTTCTCCCC TGATGTTGGC TGCAATGAAT 
GGACATGTTC CTGCAGTAAA ATTGCTGCTC GATATGGGTT CAGACATTAA TGCCCAAATA 
GAGACCAATC GGAACACGGC TCTCACCCTG GCCTGTTTCC AGGGCCGAGC AGAAGTAGTG 
AGTTTGCTTC TGGACCGAAA AGCCAATGTT GAACATAGGG CAAAGACGGG TCTTACCCCC 
TTGATGGAAG CAGCTTCTGG AGGGTATGCA GAGGTTGGAA GAGTTCTTCT TGATAAAGGA 
GCAGATGTTA ATGCTCCCCC TGTGCCTTCC TCAAGAGATA CTGCTTTAAC AATAGCAGCA 
GACAAAGGTC ACTACAAATT TTGTGAACTC CTGATTCATA GGGGAGCCCA CATTGATGTT 
CGTAACAAAA AGGGAAATAC GCCACTTTGG CTGGCATCCA ATGGAGGTCA TTTTGATGTT 
GTGCAGTTGC TAGTGCAAGC AGGTGCTGAT GTGGATGCAG CAGATAACCG GAAAATCACA 
CCTCTTATGT CAGCATTTCG CAAGGGTCAT GTAAAAGTTG TTCAATATTT GGTAAAGGAA 
GTAAATCAGT TCCCTTCTGA TATAGAATGC ATGAGATACA TAGCAACAAT TACAGATAAG 
GAACTGTTGA AAAAATGTCA TCAATGTGTC GAAACCATTG TGAAGGCTAA AGACCAGCAA 
GCTGCAGAAG CAAATAAGAA TGCGAGTATT CTTTTAAAGG AACTTGATCT GGAAAAGTCA 
AGAGAAGAGA GCAGAAAGCA GGCTCTTGCT GCTAAAAGAG AAAAAAGAAA AGAAAAGAGA 
AAAAAGAAAA AAGAGGAACA GAAAAGGAAA CAGGAAGAAG ATGAAGAAAA CAAACCTAAG 
GAGAATTCGG AACTACCAGA GGATGAAGAT GAAGAGGAGA ATGATGAAGA TGTGGAGCAA 
GAAGTTCCCA TAGAACCTCC TAGTGCAACC ACCACCACTA CGATTGGAAT CTCTGCAACA 
TCTGCAACAT TCACAAATGT GTTTGGGAAA AAAAGGGCCA ATGTGGTGAC AACTCCCAGC 
ACCAATCGGA AAAATAAGAA GAACAAAACA AAAGAAACCC CTCCTACAGC ACATTTAATT 
TTACCAGAAC AACATATGTC TTTAGCCCAA CAAAAGGCAG ATAAAAATAA AATAAATGGA 
GAACCTAGAG GTGGTGGTGC AGGTGGGAAT AGTGATTCAG ATAACTTGGA CAGCACAGAC 
TGCAACAGTG AGAGTAGCAG TGGTGGTAAA AGCCAAGAGT TAAATTTTGT GATGGATGTG 
AATTCCTCTA AATACCCCTC ACTGCTCCTT CATTCCCAAG AAGAAAAGAC AAGTACTGCT 
ACTTCCAAAA CTCAGACACG ACTTGAAGGT GAAGTGACTC CTAATTCCTT GTCAACCAGC 
TACAAGACAG TGTCATTGCC ATTAAGCTCT CCAAACATAA AGCTGAATCT CACTAGCCCT 
AAAAGGGGTC AGAAAAGAGA AGAAGGGTGG AAAGAAGTTG TACGAAGGTC AAAGAAATTG 
TCTGTTCCAG CCTCAGTGGT GTCGAGGATA ATGGGAAGAG GAGGATGCAA CATCACTGCA 
ATACAGGATG TTACTGGTGC CCATATTGAT GTGGATAAAC AAAAAGATAA GAATGGCGAG 
AGAATGATCA CAATAAGGGG TGGCACAGAA TCAACAAGAT ATGCAGTTCA ACTAATCAAT 
GCACTCATTC AAGATCCTGC TAAGGAACTG GAAGACTTGA TTCCTAAAAA TCATATCAGA 
ACACCTGCCA GCACCAAATC AATTCATGCT AACTTCTCAT CTGGAGTAGG TACCACAGCA 
GCTTCCAGTA AAAATGCATT TCCTTTGGGT GCTCCAACTC TTGTAACTTC ACAGGCAACA 
ACGTTATCTA CGTTCCAGCC CGCTAATAAA CTTAATAAGA ATGTTCCAAC AAATGTACGT 
TCTTCTTTCC CAGTTTCTCT ACCCTTAGCT TATCCTCACC CTCATTTTGC CCTGCTGGCT 
GCTCAAACTA TGCAACAGAT TCGGCATCCT CGCTTACCCA TGGCCCAGTT TGGAGGAACC 
TTCTCACCTT CTCCTAACAC ATGGGGACCA TTCCCAGTGA GACCTGTGAA TCCTGGCAAC 
ACAAATAGCT CTCCAAAGCA TAATAACACA AGCCGTCTAC CTAACCAGAA CGGGACTGTT 
TTACCCTCAG AGTCTGCTGG ACTAGCTACT GCCAGTTGTC CTATCACTGT CTCTTCTGTA 
GTTGCTGCCA GTCAGCAACT GTGTGTCACT AATACCCGGA CTCCTTCATC AGTCAGAAAG 
CAGTTGTTTG CCTGTGTGCC TAAGACAAGT CCTCCAGCAA CAGTGATTTC TTCTGTGACA 
AGCACTTGTA GTTCCCTGCC TTCTGTCTCC TCTGCACCTA TCACTAGCGG GCAAGCTCCC 
ACCACATTTC TACCTGCAAG TACTTCTCAA GCACAGCTTT CTTCACAAAA GATGGAGTCT 
TTCTCTGCTG TGCCACCCAC CAAAGAGAAA GTGTCCACAC AGGACCAGCC CATGGCAAAC 
CTATGTACCC CATCTTCAAC TGCAAACAGT TGCAGTAGCT CTGCCAGCAA CACCCCGGGA 
GCTCCAGAAA CTCACCCATC CAGTAGTCCC ACTCCTACTT CCAGTAACAC ACAAGAGGAG 
GCACAGCCAT CCAGTGTGTC TGATTTAAGT CCTATGTCAA TGCCTTTTGC ATCTAACTCA 
GAACCTGCTC CATTGACTTT GACATCACCC AGAATGGTTG CTGCTGATAA TCAGGACACC 
AGTAATTTAC CTCAGTTAGC TGTACCAGCA CCTCGAGTTT CTCATCGAAT GCAGCCCAGA 
GGTTCTTTTT ACTCCATGGT ACCAAATGCA ACTATTCACC AGGATCCCCA GTCTATTTTT 
GTTACGAATC CAGTTACTTT AACACCACCT CAAGGCCCAC CAGCTGCAGT GCAGCTTTCT 
TCAGCTGTGA ACATTATGAA TGGTTCTCAG ATGCACATAA ACCCAGCAAA TAAGTCTTTG 
CCACCTACAT TTGGCCCAGC CACACTTTTC AATCACTTCA GCAGTCTTTT TGATAGTAGT 
CAGGTGCCAG CTAACCAGGG CTGGGGAGAT GGTCCACTGT CCTCACGAGT TGCTACAGAT 
GCCTCTTTCA CTGTTCAGTC AGCGTTCCTG GGTAACTCAG TGCTTGGACA CTTGGAAAAC 
ATGCACCCTG ATAACTCAAA GGCACCTGGC TTCAGACCAC CTTCCCAGCG AGTTTCTACT 
AGTCCAGTTG GGTTACCATC CATTGACCCA TCAGGCAGCT CCCCATCTTC CTCTTCTGCT 
CCTCTGGCAA GTTTTTCCGG CATACCAGGA ACAAGGGTTT TCCTGCAAGG GCCAGCTCCT 
GTTGGGACTC CTAGTTTCAA CAGACAACAT TTTTCTCCCC ATCCTTGGAC AAGCGCCTCA 
AACTCATCCA CTTCTGCCCC ACCAACGTTG GGCCAACCAA AAGGAGTCAG TGCCAGTCAA 
GATCGAAAGA TACCTCCCCC AATTGGAACA GAGAGACTGG CCCGAATTCG GCAAGGAGGG 
TCTGTTGCAC AAGCCCCGGC GGGGACCAGT TTTGTCGCTC CCGTTGGACA CAGTGGAATC 
TGGTCATTTG GTGTCAATGC TGTGTCAGAA GGCTTATCAG GTTGGTCGCA ATCTGTGATG 
GGGAACCATC CAATGCATCA ACAATTATCA GACCCAAGCA CATTCTCCCA ACATCAGCCA 
ATGGAGAGAG ATGATTCTGG AATGGTAGCC CCCTCTAACA TTTTTCATCA GCCTATGGCA 
AGTGGTTTTG TGGATTTTTC TAAAGGTCTG CCAATTTCCA TGTATGGAGG CACCATAATA 
CCCTCTCATC CTCAGCTTGC TGATGTTCCA GGAGGCCCTC TGTTTAATGG ACTTCACAAT 
CCAGATCCTG CTTGGAACCC TATGATAAAA GTTATCCAAA ATTCAACTGA ATGCACTGAT 
GCCCAGCAGG CCAGTCTGCT TCCTTCAGTC CCTGCTCTCA AAGGGGAAAT CCCATCACCT 
CAGCTAACCA GACCGAAGAA GAGAATTGGA CGGCCGATGG TGGCCTCTCC TAACCAGAGG 
CACCAGGATC ATCTACGACC GAAAGTTCCT GCTGGAGTGC AAGAACTCAC CCATTGCCCG 
GACACCCCCC TGCTGCCTCC CTCAGATTCC CGGGGTCACA ACTCCTCCAA CAGCCCCTCT 
CTCCAAGCTG GAGGAGCTGA AGGAGCAGGA GACAGAGGAA GAGATACCCG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065741.3
CDS	155..8008
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLSTADGKAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLEHESEGGRTPLMKAARAGHLCTVQFLISKGANVNRATANNDHTVVSLACAGGHLAVVELLLAHGADPTHRLKDGSTMLIEAAKGGHTNVVSYLLDYPNNVLSVPTTDVSQLPPPSQDQSQVPRVPTHTLAMVVPPQEPDRTSQENSPALLGVQKGTSKQKSSSLQVADQDLLPSFHPYQPLECIVEETEGKLNELGQRISAIEKAQLKSLELIQGEPLNKDKIEELKKNREEQVQKKKKILKELQKVERQLQMKTQQQFTKEYLETKGQKDTVSLHQQCSHRGVFPEGEGDGSLPEDHFSELPQVDTILFKDNDVDDEQQSPPSAEQIDFVPVQPLSSPQCNFSSDLGSNGTNSLELQKVSGNQQIVGQPQIAITGHDQGLLVQEPDGLMVATPAQTLTDTLDDLIAAVSTRVPTGSNSSSQTTECLTPESCSQTTSNVASQSMPPVYPSVDIDAHTESNHDTALTLACAGGHEELVSVLIARDAKIEHRDKKGFTPLILAATAGHVGVVEILLDKGGDIEAQSERTKDTPLSLACSGGRQEVVDLLLARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHVPAVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVEHRAKTGLTPLMEAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLIHRGAHIDVRNKKGNTPLWLASNGGHFDVVQLLVQAGADVDAADNRKITPLMSAFRKGHVKVVQYLVKEVNQFPSDIECMRYIATITDKELLKKCHQCVETIVKAKDQQAAEANKNASILLKELDLEKSREESRKQALAAKREKRKEKRKKKKEEQKRKQEEDEENKPKENSELPEDEDEEENDEDVEQEVPIEPPSATTTTTIGISATSATFTNVFGKKRANVVTTPSTNRKNKKNKTKETPPTAHLILPEQHMSLAQQKADKNKINGEPRGGGAGGNSDSDNLDSTDCNSESSSGGKSQELNFVMDVNSSKYPSLLLHSQEEKTSTATSKTQTRLEGEVTPNSLSTSYKTVSLPLSSPNIKLNLTSPKRGQKREEGWKEVVRRSKKLSVPASVVSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQDPAKELEDLIPKNHIRTPASTKSIHANFSSGVGTTAASSKNAFPLGAPTLVTSQATTLSTFQPANKLNKNVPTNVRSSFPVSLPLAYPHPHFALLAAQTMQQIRHPRLPMAQFGGTFSPSPNTWGPFPVRPVNPGNTNSSPKHNNTSRLPNQNGTVLPSESAGLATASCPITVSSVVAASQQLCVTNTRTPSSVRKQLFACVPKTSPPATVISSVTSTCSSLPSVSSAPITSGQAPTTFLPASTSQAQLSSQKMESFSAVPPTKEKVSTQDQPMANLCTPSSTANSCSSSASNTPGAPETHPSSSPTPTSSNTQEEAQPSSVSDLSPMSMPFASNSEPAPLTLTSPRMVAADNQDTSNLPQLAVPAPRVSHRMQPRGSFYSMVPNATIHQDPQSIFVTNPVTLTPPQGPPAAVQLSSAVNIMNGSQMHINPANKSLPPTFGPATLFNHFSSLFDSSQVPANQGWGDGPLSSRVATDASFTVQSAFLGNSVLGHLENMHPDNSKAPGFRPPSQRVSTSPVGLPSIDPSGSSPSSSSAPLASFSGIPGTRVFLQGPAPVGTPSFNRQHFSPHPWTSASNSSTSAPPTLGQPKGVSASQDRKIPPPIGTERLARIRQGGSVAQAPAGTSFVAPVGHSGIWSFGVNAVSEGLSGWSQSVMGNHPMHQQLSDPSTFSQHQPMERDDSGMVAPSNIFHQPMASGFVDFSKGLPISMYGGTIIPSHPQLADVPGGPLFNGLHNPDPAWNPMIKVIQNSTECTDAQQASLLPSVPALKGEIPSPQLTRPKKRIGRPMVASPNQRHQDHLRPKVPAGVQELTHCPDTPLLPPSDSRGHNSSNSPSLQAGGAEGAGDRGRDTR

Target ORF information:

RefSeq Version	NM_020690.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ANKHD1-EIF4EBP3 readthrough (ANKHD1-EIF4EBP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020690.5

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGAACATGA ATCTGAAGGT 
GGAAGAACAC CTTTGATGAA AGCTGCAAGA GCTGGTCATT TGTGCACTGT GCAGTTTCTT 
ATTAGCAAAG GTGCCAATGT TAACAGGGCT ACAGCCAATA ATGATCATAC AGTAGTGTCG 
CTGGCATGTG CAGGAGGCCA CCTGGCAGTT GTTGAGCTTC TCTTGGCTCA TGGGGCTGAC 
CCTACTCATC GACTCAAGGA TGGTTCAACA ATGCTCATTG AAGCTGCAAA GGGTGGCCAT 
ACTAATGTAG TTTCTTATCT GTTGGATTAT CCAAATAATG TTCTGTCAGT TCCCACCACA 
GATGTGTCTC AGCTCCCTCC ACCTTCTCAA GATCAGTCTC AGGTGCCACG TGTGCCAACG 
CATACACTTG CCATGGTTGT ACCTCCCCAG GAACCTGACA GAACTTCACA GGAGAACTCT 
CCTGCCCTTT TAGGAGTGCA AAAAGGTACA TCCAAGCAGA AGTCCAGTTC CCTCCAGGTA 
GCAGATCAGG ACCTACTGCC ATCTTTTCAC CCATACCAGC CTTTGGAGTG CATAGTAGAG 
GAGACTGAAG GCAAGCTGAA TGAACTGGGA CAAAGAATTA GTGCTATTGA AAAAGCACAG 
CTTAAGTCAC TGGAGTTAAT TCAAGGTGAA CCTCTGAACA AAGATAAGAT AGAAGAACTT 
AAAAAGAACA GAGAAGAGCA AGTCCAGAAG AAGAAGAAAA TATTGAAAGA ACTGCAGAAA 
GTGGAAAGGC AGTTGCAGAT GAAAACACAG CAGCAATTTA CCAAAGAATA CTTGGAAACC 
AAAGGTCAGA AAGACACAGT GTCTCTACAC CAACAGTGCT CTCATAGAGG AGTCTTCCCA 
GAAGGGGAAG GAGATGGTAG TCTCCCAGAG GATCACTTTT CAGAGTTACC TCAGGTTGAC 
ACAATCTTAT TTAAAGATAA TGATGTTGAT GATGAGCAAC AGTCTCCACC ATCGGCAGAA 
CAGATTGATT TTGTCCCAGT CCAGCCTTTA TCATCTCCAC AGTGTAACTT TTCCAGTGAC 
TTAGGTTCTA ATGGGACAAA TTCTCTTGAA CTTCAGAAAG TATCAGGTAA TCAGCAGATT 
GTAGGACAGC CTCAGATTGC TATTACTGGA CATGATCAGG GGCTGTTAGT TCAAGAACCA 
GATGGACTAA TGGTTGCAAC TCCAGCTCAG ACGCTTACCG ACACTCTTGA TGACCTGATA 
GCAGCTGTGA GTACCAGAGT GCCCACTGGT TCCAACAGTT CTTCTCAGAC CACAGAGTGT 
CTTACACCTG AATCCTGTTC GCAGACTACA AGCAATGTGG CTTCCCAATC GATGCCTCCT 
GTGTATCCTT CAGTTGACAT TGATGCACAT ACTGAGAGCA ATCATGACAC AGCATTAACA 
CTAGCTTGTG CAGGTGGTCA TGAAGAACTT GTATCTGTGC TCATTGCACG GGATGCCAAA 
ATTGAACACA GAGACAAAAA AGGTTTCACA CCACTAATCC TGGCAGCAAC AGCAGGGCAT 
GTTGGAGTTG TTGAAATCCT TTTGGATAAA GGTGGAGATA TAGAAGCACA GTCTGAACGA 
ACTAAGGATA CTCCGCTTTC ATTGGCATGT TCTGGTGGAC GTCAGGAGGT GGTAGACTTG 
CTGCTGGCTC GAGGTGCAAA TAAAGAACAT AGGAACGTAT CTGATTATAC ACCACTGAGT 
CTAGCTGCGT CTGGAGGATA TGTTAATATC ATTAAGATTC TGCTTAATGC TGGGGCAGAA 
ATTAATTCAA GGACTGGGAG TAAACTAGGT ATTTCTCCCC TGATGTTGGC TGCAATGAAT 
GGACATGTTC CTGCAGTAAA ATTGCTGCTC GATATGGGTT CAGACATTAA TGCCCAAATA 
GAGACCAATC GGAACACGGC TCTCACCCTG GCCTGTTTCC AGGGCCGAGC AGAAGTAGTG 
AGTTTGCTTC TGGACCGAAA AGCCAATGTT GAACATAGGG CAAAGACGGG TCTTACCCCC 
TTGATGGAAG CAGCTTCTGG AGGGTATGCA GAGGTTGGAA GAGTTCTTCT TGATAAAGGA 
GCAGATGTTA ATGCTCCCCC TGTGCCTTCC TCAAGAGATA CTGCTTTAAC AATAGCAGCA 
GACAAAGGTC ACTACAAATT TTGTGAACTC CTGATTCATA GGGGAGCCCA CATTGATGTT 
CGTAACAAAA AGGGAAATAC GCCACTTTGG CTGGCATCCA ATGGAGGTCA TTTTGATGTT 
GTGCAGTTGC TAGTGCAAGC AGGTGCTGAT GTGGATGCAG CAGATAACCG GAAAATCACA 
CCTCTTATGT CAGCATTTCG CAAGGGTCAT GTAAAAGTTG TTCAATATTT GGTAAAGGAA 
GTAAATCAGT TCCCTTCTGA TATAGAATGC ATGAGATACA TAGCAACAAT TACAGATAAG 
GAACTGTTGA AAAAATGTCA TCAATGTGTC GAAACCATTG TGAAGGCTAA AGACCAGCAA 
GCTGCAGAAG CAAATAAGAA TGCGAGTATT CTTTTAAAGG AACTTGATCT GGAAAAGTCA 
AGAGAAGAGA GCAGAAAGCA GGCTCTTGCT GCTAAAAGAG AAAAAAGAAA AGAAAAGAGA 
AAAAAGAAAA AAGAGGAACA GAAAAGGAAA CAGGAAGAAG ATGAAGAAAA CAAACCTAAG 
GAGAATTCGG AACTACCAGA GGATGAAGAT GAAGAGGAGA ATGATGAAGA TGTGGAGCAA 
GAAGTTCCCA TAGAACCTCC TAGTGCAACC ACCACCACTA CGATTGGAAT CTCTGCAACA 
TCTGCAACAT TCACAAATGT GTTTGGGAAA AAAAGGGCCA ATGTGGTGAC AACTCCCAGC 
ACCAATCGGA AAAATAAGAA GAACAAAACA AAAGAAACCC CTCCTACAGC ACATTTAATT 
TTACCAGAAC AACATATGTC TTTAGCCCAA CAAAAGGCAG ATAAAAATAA AATAAATGGA 
GAACCTAGAG GTGGTGGTGC AGGTGGGAAT AGTGATTCAG ATAACTTGGA CAGCACAGAC 
TGCAACAGTG AGAGTAGCAG TGGTGGTAAA AGCCAAGAGT TAAATTTTGT GATGGATGTG 
AATTCCTCTA AATACCCCTC ACTGCTCCTT CATTCCCAAG AAGAAAAGAC AAGTACTGCT 
ACTTCCAAAA CTCAGACACG ACTTGAAGGT GAAGTGACTC CTAATTCCTT GTCAACCAGC 
TACAAGACAG TGTCATTGCC ATTAAGCTCT CCAAACATAA AGCTGAATCT CACTAGCCCT 
AAAAGGGGTC AGAAAAGAGA AGAAGGGTGG AAAGAAGTTG TACGAAGGTC AAAGAAATTG 
TCTGTTCCAG CCTCAGTGGT GTCGAGGATA ATGGGAAGAG GAGGATGCAA CATCACTGCA 
ATACAGGATG TTACTGGTGC CCATATTGAT GTGGATAAAC AAAAAGATAA GAATGGCGAG 
AGAATGATCA CAATAAGGGG TGGCACAGAA TCAACAAGAT ATGCAGTTCA ACTAATCAAT 
GCACTCATTC AAGATCCTGC TAAGGAACTG GAAGACTTGA TTCCTAAAAA TCATATCAGA 
ACACCTGCCA GCACCAAATC AATTCATGCT AACTTCTCAT CTGGAGTAGG TACCACAGCA 
GCTTCCAGTA AAAATGCATT TCCTTTGGGT GCTCCAACTC TTGTAACTTC ACAGGCAACA 
ACGTTATCTA CGTTCCAGCC CGCTAATAAA CTTAATAAGA ATGTTCCAAC AAATGTACGT 
TCTTCTTTCC CAGTTTCTCT ACCCTTAGCT TATCCTCACC CTCATTTTGC CCTGCTGGCT 
GCTCAAACTA TGCAACAGAT TCGGCATCCT CGCTTACCCA TGGCCCAGTT TGGAGGAACC 
TTCTCACCTT CTCCTAACAC ATGGGGACCA TTCCCAGTGA GACCTGTGAA TCCTGGCAAC 
ACAAATAGCT CTCCAAAGCA TAATAACACA AGCCGTCTAC CTAACCAGAA CGGGACTGTT 
TTACCCTCAG AGTCTGCTGG ACTAGCTACT GCCAGTTGTC CTATCACTGT CTCTTCTGTA 
GTTGCTGCCA GTCAGCAACT GTGTGTCACT AATACCCGGA CTCCTTCATC AGTCAGAAAG 
CAGTTGTTTG CCTGTGTGCC TAAGACAAGT CCTCCAGCAA CAGTGATTTC TTCTGTGACA 
AGCACTTGTA GTTCCCTGCC TTCTGTCTCC TCTGCACCTA TCACTAGCGG GCAAGCTCCC 
ACCACATTTC TACCTGCAAG TACTTCTCAA GCACAGCTTT CTTCACAAAA GATGGAGTCT 
TTCTCTGCTG TGCCACCCAC CAAAGAGAAA GTGTCCACAC AGGACCAGCC CATGGCAAAC 
CTATGTACCC CATCTTCAAC TGCAAACAGT TGCAGTAGCT CTGCCAGCAA CACCCCGGGA 
GCTCCAGAAA CTCACCCATC CAGTAGTCCC ACTCCTACTT CCAGTAACAC ACAAGAGGAG 
GCACAGCCAT CCAGTGTGTC TGATTTAAGT CCTATGTCAA TGCCTTTTGC ATCTAACTCA 
GAACCTGCTC CATTGACTTT GACATCACCC AGAATGGTTG CTGCTGATAA TCAGGACACC 
AGTAATTTAC CTCAGTTAGC TGTACCAGCA CCTCGAGTTT CTCATCGAAT GCAGCCCAGA 
GGTTCTTTTT ACTCCATGGT ACCAAATGCA ACTATTCACC AGGATCCCCA GTCTATTTTT 
GTTACGAATC CAGTTACTTT AACACCACCT CAAGGCCCAC CAGCTGCAGT GCAGCTTTCT 
TCAGCTGTGA ACATTATGAA TGGTTCTCAG ATGCACATAA ACCCAGCAAA TAAGTCTTTG 
CCACCTACAT TTGGCCCAGC CACACTTTTC AATCACTTCA GCAGTCTTTT TGATAGTAGT 
CAGGTGCCAG CTAACCAGGG CTGGGGAGAT GGTCCACTGT CCTCACGAGT TGCTACAGAT 
GCCTCTTTCA CTGTTCAGTC AGCGTTCCTG GGTAACTCAG TGCTTGGACA CTTGGAAAAC 
ATGCACCCTG ATAACTCAAA GGCACCTGGC TTCAGACCAC CTTCCCAGCG AGTTTCTACT 
AGTCCAGTTG GGTTACCATC CATTGACCCA TCAGGCAGCT CCCCATCTTC CTCTTCTGCT 
CCTCTGGCAA GTTTTTCCGG CATACCAGGA ACAAGGGTTT TCCTGCAAGG GCCAGCTCCT 
GTTGGGACTC CTAGTTTCAA CAGACAACAT TTTTCTCCCC ATCCTTGGAC AAGCGCCTCA 
AACTCATCCA CTTCTGCCCC ACCAACGTTG GGCCAACCAA AAGGAGTCAG TGCCAGTCAA 
GATCGAAAGA TACCTCCCCC AATTGGAACA GAGAGACTGG CCCGAATTCG GCAAGGAGGG 
TCTGTTGCAC AAGCCCCGGC GGGGACCAGT TTTGTCGCTC CCGTTGGACA CAGTGGAATC 
TGGTCATTTG GTGTCAATGC TGTGTCAGAA GGCTTATCAG GTTGGTCGCA ATCTGTGATG 
GGGAACCATC CAATGCATCA ACAATTATCA GACCCAAGCA CATTCTCCCA ACATCAGCCA 
ATGGAGAGAG ATGATTCTGG AATGGTAGCC CCCTCTAACA TTTTTCATCA GCCTATGGCA 
AGTGGTTTTG TGGATTTTTC TAAAGGTCTG CCAATTTCCA TGTATGGAGG CACCATAATA 
CCCTCTCATC CTCAGCTTGC TGATGTTCCA GGAGGCCCTC TGTTTAATGG ACTTCACAAT 
CCAGATCCTG CTTGGAACCC TATGATAAAA GTTATCCAAA ATTCAACTGA ATGCACTGAT 
GCCCAGCAGG CCAGTCTGCT TCCTTCAGTC CCTGCTCTCA AAGGGGAAAT CCCATCACCT 
CAGCTAACCA GACCGAAGAA GAGAATTGGA CGGCCGATGG TGGCCTCTCC TAACCAGAGG 
CACCAGGATC ATCTACGACC GAAAGTTCCT GCTGGAGTGC AAGAACTCAC CCATTGCCCG 
GACACCCCCC TGCTGCCTCC CTCAGATTCC CGGGGTCACA ACTCCTCCAA CAGCCCCTCT 
CTCCAAGCTG GAGGAGCTGA AGGAGCAGGA GACAGAGGAA GAGATACCCG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of conjoined genes: another mechanism for gene regulation in eukaryotes.
PloS one5(10)e13284(2010 Oct)
Prakash T,Sharma VK,Adati N,Ozawa R,Kumar N,Nishida Y,Fujikake T,Takeda T,Taylor TD

Characterisation of a new splice variant of MASK-BP3(ARF) and MASK human genes, and their expression patterns during haematopoietic cell differentiation.
Gene363113-22(2005 Dec)
Santos Duarte Ada S,Traina F,Favaro PM,Bassères DS,de Carvalho IC,Medina Sde S,Costa FF,Saad ST

Gene fusion and overlapping reading frames in the mammalian genes for 4E-BP3 and MASK.
The Journal of biological chemistry278(52)52290-7(2003 Dec)
Poulin F,Brueschke A,Sonenberg N

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ANKHD1-EIF4EBP3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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