UBE2NL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2NL
Entrez Gene ID	389898
Full Name	ubiquitin conjugating enzyme E2 N like (gene/pseudogene)
Synonyms	Li174
General protein information	Preferred Names ubiquitin conjugating enzyme E2 N like (gene/pseudogene) Names putative ubiquitin-conjugating enzyme E2 N-like epididymis tissue protein Li 174 ubiquitin conjugating enzyme E2N-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq27.3
Summary	This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. This locus represents a polymorphic pseudogene, where some individuals contain an allele that can encode a full-length protein, while others have a non-functional allele containing a premature stop codon (reference SNP rs237520) that truncates the coding sequence. [provided by RefSeq, Jun 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012989.2	NP_001013007.1	putative ubiquitin-conjugating enzyme E2 N-like

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LOC100856713	XP_003639557.1
Homo sapiens (human)	UBE2NL	NP_001013007.1
Pan troglodytes (chimpanzee)	UBE2NL	XP_003954503.1
Macaca mulatta (Rhesus monkey)	LOC703337	XP_001094543.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins.
Li J, Liu F, Wang H, Liu X, Liu J, Li N, Wan F, Wang W, Zhang C, Jin S, Liu J, Zhu P, Liu Y
Molecular & cellular proteomics : MCP9(11)2517-28(2010 Nov)

A systematic, large-scale resequencing screen of X-chromosome coding exons in mental retardation.
Tarpey PS, Smith R, Pleasance E, Whibley A, Edkins S, Hardy C, O'Meara S, Latimer C, Dicks E, Menzies A, Stephens P, Blow M, Greenman C, Xue Y, Tyler-Smith C, Thompson D, Gray K, Andrews J, Barthorpe S, Buck G, Cole J, Dunmore R, Jones D, Maddison M, Mironenko T, Turner R, Turrell K, Varian J, West S, Widaa S, Wray P, Teague J, Butler A, Jenkinson A, Jia M, Richardson D, Shepherd R, Wooster R, Tejada MI, Martinez F, Carvill G, Goliath R, de Brouwer AP, van Bokhoven H, Van Esch H, Chelly J, Raynaud M, Ropers HH, Abidi FE, Srivastava AK, Cox J, Luo Y, Mallya U, Moon J, Parnau J, Mohammed S, Tolmie JL, Shoubridge C, Corbett M, Gardner A, Haan E, Rujirabanjerd S, Shaw M, Vandeleur L, Fullston T, Easton DF, Boyle J, Partington M, Hackett A, Field M, Skinner C, Stevenson RE, Bobrow M, Turner G, Schwartz CE, Gecz J, Raymond FL, Futreal PA, Stratton MR
Nature genetics41(5)535-43(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UBE2NL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2NL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu27981	NM_001012989.2 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 N like (gene/pseudogene) (UBE2NL), transcript variant coding, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2NL ( NM_001012989.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013007.1 Homo sapiens ubiquitin conjugating enzyme E2 N like (gene/pseudogene) (UBE2NL), transcript variant coding, mRNA.

Price & Availability↑

CloneID	OHu27981
Clone ID Related Accession (Same CDS sequence)	NM_001012989.2
Accession Version	NM_001012989.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556553600000
Organism	Homo sapiens(human)
Product	putative ubiquitin-conjugating enzyme E2 N-like
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from GU727624.1 and AL135920.13. This sequence is a reference standard in the RefSeqGene project. On Aug 27, 2013 this sequence version replaced NM_001012989.1. Transcript Variant: This variant (coding) represents the functional allele, and can encode a full-length protein. ##Evidence-Data-START## Transcript is intronless :: GU727624.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## polymorphic pseudogene :: PMID: 19377476 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGCCGAGC TGCCCCACAG GATCATCAAG GAAACCCAGC GTTTGCTGGC AGAGCCAGTT 
CCTGGCATCA AAGCAGAACC AGATGAAAGC AACGCCCGTT ATTTTCATGT GGTCATTGCT 
GGGGAATCAA AGGATTCCCC CTTTGAGGGA GGGACTTTTA AACGTGAACT ATTACTTGCA 
GAAGAATACC CAATGGCAGC CCCTAAAGTA CGTTTCATGA CCAAAATTTA TCATCCAAAT 
GTAGACAAGT TGGAAAGAAT AAGTTTAGAT ATTTTGAAAG ATAAGTGGTC CCCAGCCCTG 
CAGATCCGCA CAGTTCTGCT ATCGATCCAG GCCTTGTTAA ATGCTCCCAA TCCAGATGAT 
CCATTAGCAA ATGATGTAGT GGAGCAGTGG AAGACCAACG AAGCCCAAGC CATTGAAACA 
GCTAGAGCAT GGACTAGGCT ATATGCCATG AATAGTATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013007.1
CDS	31..492
Translation	MAELPHRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGESKDSPFEGGTFKRELLLAEEYPMAAPKVRFMTKIYHPNVDKLERISLDILKDKWSPALQIRTVLLSIQALLNAPNPDDPLANDVVEQWKTNEAQAIETARAWTRLYAMNSI

Target ORF information:

RefSeq Version	NM_001012989.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 N like (gene/pseudogene) (UBE2NL), transcript variant coding, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012989.2

ORF Insert Sequence:

ATGGCCGAGC TGCCCCACAG GATCATCAAG GAAACCCAGC GTTTGCTGGC AGAGCCAGTT 
CCTGGCATCA AAGCAGAACC AGATGAAAGC AACGCCCGTT ATTTTCATGT GGTCATTGCT 
GGGGAATCAA AGGATTCCCC CTTTGAGGGA GGGACTTTTA AACGTGAACT ATTACTTGCA 
GAAGAATACC CAATGGCAGC CCCTAAAGTA CGTTTCATGA CCAAAATTTA TCATCCAAAT 
GTAGACAAGT TGGAAAGAAT AAGTTTAGAT ATTTTGAAAG ATAAGTGGTC CCCAGCCCTG 
CAGATCCGCA CAGTTCTGCT ATCGATCCAG GCCTTGTTAA ATGCTCCCAA TCCAGATGAT 
CCATTAGCAA ATGATGTAGT GGAGCAGTGG AAGACCAACG AAGCCCAAGC CATTGAAACA 
GCTAGAGCAT GGACTAGGCT ATATGCCATG AATAGTATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins.
Molecular & cellular proteomics : MCP9(11)2517-28(2010 Nov)
Li J,Liu F,Wang H,Liu X,Liu J,Li N,Wan F,Wang W,Zhang C,Jin S,Liu J,Zhu P,Liu Y

A systematic, large-scale resequencing screen of X-chromosome coding exons in mental retardation.
Nature genetics41(5)535-43(2009 May)
Tarpey PS,Smith R,Pleasance E,Whibley A,Edkins S,Hardy C,O'Meara S,Latimer C,Dicks E,Menzies A,Stephens P,Blow M,Greenman C,Xue Y,Tyler-Smith C,Thompson D,Gray K,Andrews J,Barthorpe S,Buck G,Cole J,Dunmore R,Jones D,Maddison M,Mironenko T,Turner R,Turrell K,Varian J,West S,Widaa S,Wray P,Teague J,Butler A,Jenkinson A,Jia M,Richardson D,Shepherd R,Wooster R,Tejada MI,Martinez F,Carvill G,Goliath R,de Brouwer AP,van Bokhoven H,Van Esch H,Chelly J,Raynaud M,Ropers HH,Abidi FE,Srivastava AK,Cox J,Luo Y,Mallya U,Moon J,Parnau J,Mohammed S,Tolmie JL,Shoubridge C,Corbett M,Gardner A,Haan E,Rujirabanjerd S,Shaw M,Vandeleur L,Fullston T,Easton DF,Boyle J,Partington M,Hackett A,Field M,Skinner C,Stevenson RE,Bobrow M,Turner G,Schwartz CE,Gecz J,Raymond FL,Futreal PA,Stratton MR

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UBE2NL cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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