HPD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HPD
Entrez Gene ID	3242
Full Name	4-hydroxyphenylpyruvate dioxygenase
Synonyms	4-HPPD,4HPPD,GLOD3,HPPDASE,PPD
General protein information	Preferred Names 4-hydroxyphenylpyruvate dioxygenase Names 4-hydroxyphenylpyruvate dioxygenase 4-hydroxyphenylpyruvic acid oxidase glyoxalase domain containing 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Summary	The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010].
Disorder MIM:	609695
Disorder Html:	Tyrosinemia, type III, 276710 (3); Hawkinsinuria, 140350 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001171993.1	NP_001165464.1	4-hydroxyphenylpyruvate dioxygenase isoform 2
NM_002150.2	NP_002141.1	4-hydroxyphenylpyruvate dioxygenase isoform 1
NM_002150.3	NP_002141.2	4-hydroxyphenylpyruvate dioxygenase isoform 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	HPD	XP_001096175.1
Mus musculus (house mouse)	Hpd	NP_032303.1
Caenorhabditis elegans (roundworm)	hpd-1	NP_499324.1
Xenopus tropicalis (tropical clawed frog)	hpd	NP_001072376.1
Bos taurus (cattle)	HPD	NP_001015611.1
Rattus norvegicus (Norway rat)	Hpd	NP_058929.1
Gallus gallus (chicken)	HPD	NP_001264420.1
Arabidopsis thaliana (thale cress)	PDS1	NP_172144.2
Magnaporthe oryzae (rice blast fungus)	MGG_06691	XP_003709399.1
Neurospora crassa	NCU01830	XP_956614.1
Homo sapiens (human)	HPD	NP_002141.1
Pan troglodytes (chimpanzee)	HPD	XP_528667.2
Drosophila melanogaster (fruit fly)	CG11796	NP_730536.1
Canis lupus familiaris (dog)	HPD	XP_853422.1
Oryza sativa (rice)	Os02g0168100	NP_001046010.1
Danio rerio (zebrafish)	hpdb	NP_001003742.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004802	XP_318007.3

Gene Ontology
Bibliography

Related articles in PubMed

The different catalytic roles of the metal-binding ligands in human 4-hydroxyphenylpyruvate dioxygenase.
Huang CW, Liu HC, Shen CP, Chen YT, Lee SJ, Lloyd MD, Lee HJ
The Biochemical journal473(9)1179-89(2016 05)

Hawkinsinuria in two unrelated Greek newborns: identification of a novel variant, biochemical findings and treatment.
Thodi G, Schulpis KH, Dotsikas Y, Pavlides C, Molou E, Chatzidaki M, Triantafylli O, Loukas YL
Journal of pediatric endocrinology & metabolism : JPEM29(1)15-20(2016 Jan)

The interactions in the carboxyl terminus of human 4-hydroxyphenylpyruvate dioxygenase are critical to mediate the conformation of the final helix and the tail to shield the active site for catalysis.
Lin JF, Sheih YL, Chang TC, Chang NY, Chang CW, Shen CP, Lee HJ
PloS one8(8)e69733(2013)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

A genome-wide association study of metabolic traits in human urine.
Suhre K, Wallaschofski H, Raffler J, Friedrich N, Haring R, Michael K, Wasner C, Krebs A, Kronenberg F, Chang D, Meisinger C, Wichmann HE, Hoffmann W, Völzke H, Völker U, Teumer A, Biffar R, Kocher T, Felix SB, Illig T, Kroemer HK, Gieger C, Römisch-Margl W, Nauck M
Nature genetics43(6)565-9(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HPD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HPD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu107797	NM_002150.3 Latest version!	Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu09763	NM_002150.2 Latest version!	Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
OHu18126	NM_001171993.1 Latest version!	Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HPD ( NM_002150.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002141.2 Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107797
Clone ID Related Accession (Same CDS sequence)	NM_002150.3
Accession Version	NM_002150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	4-hydroxyphenylpyruvate dioxygenase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079360.28. On Nov 22, 2018 this sequence version replaced NM_002150.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.100730.1, SRR5189664.77782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155590, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000289004.8/ ENSP00000289004.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGACTT ACAGTGACAA AGGGGCAAAG CCTGAGAGAG GCCGATTCCT CCACTTCCAC 
TCTGTGACCT TCTGGGTTGG CAACGCCAAG CAGGCCACGT CATTCTACTG CAGCAAGATG 
GGCTTTGAAC CTCTAGCCTA CAGGGGCCTG GAGACCGGTT CCCGGGAGGT GGTCAGCCAT 
GTAATCAAAC AAGGGAAGAT TGTGTTTGTC CTCTCCTCAG CGCTCAACCC CTGGAACAAA 
GAGATGGGCG ATCACCTGGT GAAACACGGT GACGGAGTGA AGGACATTGC GTTCGAGGTG 
GAAGATTGTG ACTACATCGT GCAGAAAGCA CGGGAACGGG GCGCCAAAAT CATGCGGGAG 
CCCTGGGTAG AGCAAGACAA GTTTGGGAAG GTGAAGTTTG CTGTGCTGCA GACGTATGGG 
GACACCACAC ACACCCTGGT GGAGAAGATG AACTACATCG GCCAATTCTT GCCTGGATAT 
GAGGCCCCAG CGTTCATGGA CCCCCTACTT CCTAAACTGC CCAAATGCAG TCTGGAGATG 
ATCGACCACA TTGTGGGAAA CCAGCCTGAT CAGGAGATGG TGTCCGCCTC CGAATGGTAC 
CTGAAAAACC TGCAGTTCCA CCGCTTCTGG TCCGTGGATG ACACGCAGGT GCACACGGAA 
TATAGCTCTC TGCGATCCAT TGTGGTGGCC AACTATGAAG AGTCCATCAA GATGCCCATC 
AATGAGCCAG CGCCTGGCAA GAAGAAGTCC CAGATCCAGG AATATGTGGA CTATAACGGG 
GGCGCTGGGG TCCAGCACAT CGCTCTCAAG ACCGAAGACA TCATCACAGC GATTCGCCAC 
TTGAGAGAGA GAGGCCTGGA GTTCTTATCT GTTCCCTCCA CGTACTACAA ACAACTGCGG 
GAGAAGCTGA AGACGGCCAA GATCAAGGTG AAGGAGAACA TTGATGCCCT GGAGGAGCTG 
AAAATCCTGG TGGACTACGA CGAGAAAGGC TACCTCCTGC AGATCTTCAC CAAACCGGTG 
CAGGACCGGC CCACGCTCTT CCTGGAAGTC ATCCAGCGCC ACAACCACCA GGGTTTTGGA 
GCCGGCAACT TCAACTCACT GTTCAAGGCT TTCGAGGAGG AGCAGAACCT GCGGGGTAAC 
CTCACCAACA TGGAGACCAA TGGGGTGGTG CCCGGCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002141.2
CDS	37..1218
Translation	MTTYSDKGAKPERGRFLHFHSVTFWVGNAKQATSFYCSKMGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM

Target ORF information:

RefSeq Version	NM_002150.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002150.3

ORF Insert Sequence:

ATGACGACTT ACAGTGACAA AGGGGCAAAG CCTGAGAGAG GCCGATTCCT CCACTTCCAC 
TCTGTGACCT TCTGGGTTGG CAACGCCAAG CAGGCCACGT CATTCTACTG CAGCAAGATG 
GGCTTTGAAC CTCTAGCCTA CAGGGGCCTG GAGACCGGTT CCCGGGAGGT GGTCAGCCAT 
GTAATCAAAC AAGGGAAGAT TGTGTTTGTC CTCTCCTCAG CGCTCAACCC CTGGAACAAA 
GAGATGGGCG ATCACCTGGT GAAACACGGT GACGGAGTGA AGGACATTGC GTTCGAGGTG 
GAAGATTGTG ACTACATCGT GCAGAAAGCA CGGGAACGGG GCGCCAAAAT CATGCGGGAG 
CCCTGGGTAG AGCAAGACAA GTTTGGGAAG GTGAAGTTTG CTGTGCTGCA GACGTATGGG 
GACACCACAC ACACCCTGGT GGAGAAGATG AACTACATCG GCCAATTCTT GCCTGGATAT 
GAGGCCCCAG CGTTCATGGA CCCCCTACTT CCTAAACTGC CCAAATGCAG TCTGGAGATG 
ATCGACCACA TTGTGGGAAA CCAGCCTGAT CAGGAGATGG TGTCCGCCTC CGAATGGTAC 
CTGAAAAACC TGCAGTTCCA CCGCTTCTGG TCCGTGGATG ACACGCAGGT GCACACGGAA 
TATAGCTCTC TGCGATCCAT TGTGGTGGCC AACTATGAAG AGTCCATCAA GATGCCCATC 
AATGAGCCAG CGCCTGGCAA GAAGAAGTCC CAGATCCAGG AATATGTGGA CTATAACGGG 
GGCGCTGGGG TCCAGCACAT CGCTCTCAAG ACCGAAGACA TCATCACAGC GATTCGCCAC 
TTGAGAGAGA GAGGCCTGGA GTTCTTATCT GTTCCCTCCA CGTACTACAA ACAACTGCGG 
GAGAAGCTGA AGACGGCCAA GATCAAGGTG AAGGAGAACA TTGATGCCCT GGAGGAGCTG 
AAAATCCTGG TGGACTACGA CGAGAAAGGC TACCTCCTGC AGATCTTCAC CAAACCGGTG 
CAGGACCGGC CCACGCTCTT CCTGGAAGTC ATCCAGCGCC ACAACCACCA GGGTTTTGGA 
GCCGGCAACT TCAACTCACT GTTCAAGGCT TTCGAGGAGG AGCAGAACCT GCGGGGTAAC 
CTCACCAACA TGGAGACCAA TGGGGTGGTG CCCGGCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HPD ( NM_002150.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002141.1 Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09763
Clone ID Related Accession (Same CDS sequence)	NM_002150.2
Accession Version	NM_002150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514217600000
Organism	Homo sapiens(human)
Product	4-hydroxyphenylpyruvate dioxygenase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD675915.1, X72389.1 and BC024287.1. On Oct 29, 2003 this sequence version replaced NM_002150.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014790.1, AK290826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155590, SAMEA2162895 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGACTT ACAGTGACAA AGGGGCAAAG CCTGAGAGAG GCCGATTCCT CCACTTCCAC 
TCTGTGACCT TCTGGGTTGG CAACGCCAAG CAGGCCGCGT CATTCTACTG CAGCAAGATG 
GGCTTTGAAC CTCTAGCCTA CAGGGGCCTG GAGACCGGTT CCCGGGAGGT GGTCAGCCAT 
GTAATCAAAC AAGGGAAGAT TGTGTTTGTC CTCTCCTCAG CGCTCAACCC CTGGAACAAA 
GAGATGGGCG ATCACCTGGT GAAACACGGT GACGGAGTGA AGGACATTGC GTTCGAGGTG 
GAAGATTGTG ACTACATCGT GCAGAAAGCA CGGGAACGGG GCGCCAAAAT CATGCGGGAG 
CCCTGGGTAG AGCAAGACAA GTTTGGGAAG GTGAAGTTTG CTGTGCTGCA GACGTATGGG 
GACACCACAC ACACCCTGGT GGAGAAGATG AACTACATCG GCCAATTCTT GCCTGGATAT 
GAGGCCCCAG CGTTCATGGA CCCCCTACTT CCTAAACTGC CCAAATGCAG TCTGGAGATG 
ATCGACCACA TTGTGGGAAA CCAGCCTGAT CAGGAGATGG TGTCCGCCTC CGAATGGTAC 
CTGAAAAACC TGCAGTTCCA CCGCTTCTGG TCCGTGGATG ACACGCAGGT GCACACGGAA 
TATAGCTCTC TGCGATCCAT TGTGGTGGCC AACTATGAAG AGTCCATCAA GATGCCCATC 
AATGAGCCAG CGCCTGGCAA GAAGAAGTCC CAGATCCAGG AATATGTGGA CTATAACGGG 
GGCGCTGGGG TCCAGCACAT CGCTCTCAAG ACCGAAGACA TCATCACAGC GATTCGCCAC 
TTGAGAGAGA GAGGCCTGGA GTTCTTATCT GTTCCCTCCA CGTACTACAA ACAACTGCGG 
GAGAAGCTGA AGACGGCCAA GATCAAGGTG AAGGAGAACA TTGATGCCCT GGAGGAGCTG 
AAAATCCTGG TGGACTACGA CGAGAAAGGC TACCTCCTGC AGATCTTCAC CAAACCGGTG 
CAGGACCGGC CCACGCTCTT CCTGGAAGTC ATCCAGCGCC ACAACCACCA GGGTTTTGGA 
GCCGGCAACT TCAACTCACT GTTCAAGGCT TTCGAGGAGG AGCAGAACCT GCGGGGTAAC 
CTCACCAACA TGGAGACCAA TGGGGTGGTG CCCGGCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002141.1
CDS	41..1222
Translation	MTTYSDKGAKPERGRFLHFHSVTFWVGNAKQAASFYCSKMGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM

Target ORF information:

RefSeq Version	NM_002150.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002150.2

ORF Insert Sequence:

ATGACGACTT ACAGTGACAA AGGGGCAAAG CCTGAGAGAG GCCGATTCCT CCACTTCCAC 
TCTGTGACCT TCTGGGTTGG CAACGCCAAG CAGGCCGCGT CATTCTACTG CAGCAAGATG 
GGCTTTGAAC CTCTAGCCTA CAGGGGCCTG GAGACCGGTT CCCGGGAGGT GGTCAGCCAT 
GTAATCAAAC AAGGGAAGAT TGTGTTTGTC CTCTCCTCAG CGCTCAACCC CTGGAACAAA 
GAGATGGGCG ATCACCTGGT GAAACACGGT GACGGAGTGA AGGACATTGC GTTCGAGGTG 
GAAGATTGTG ACTACATCGT GCAGAAAGCA CGGGAACGGG GCGCCAAAAT CATGCGGGAG 
CCCTGGGTAG AGCAAGACAA GTTTGGGAAG GTGAAGTTTG CTGTGCTGCA GACGTATGGG 
GACACCACAC ACACCCTGGT GGAGAAGATG AACTACATCG GCCAATTCTT GCCTGGATAT 
GAGGCCCCAG CGTTCATGGA CCCCCTACTT CCTAAACTGC CCAAATGCAG TCTGGAGATG 
ATCGACCACA TTGTGGGAAA CCAGCCTGAT CAGGAGATGG TGTCCGCCTC CGAATGGTAC 
CTGAAAAACC TGCAGTTCCA CCGCTTCTGG TCCGTGGATG ACACGCAGGT GCACACGGAA 
TATAGCTCTC TGCGATCCAT TGTGGTGGCC AACTATGAAG AGTCCATCAA GATGCCCATC 
AATGAGCCAG CGCCTGGCAA GAAGAAGTCC CAGATCCAGG AATATGTGGA CTATAACGGG 
GGCGCTGGGG TCCAGCACAT CGCTCTCAAG ACCGAAGACA TCATCACAGC GATTCGCCAC 
TTGAGAGAGA GAGGCCTGGA GTTCTTATCT GTTCCCTCCA CGTACTACAA ACAACTGCGG 
GAGAAGCTGA AGACGGCCAA GATCAAGGTG AAGGAGAACA TTGATGCCCT GGAGGAGCTG 
AAAATCCTGG TGGACTACGA CGAGAAAGGC TACCTCCTGC AGATCTTCAC CAAACCGGTG 
CAGGACCGGC CCACGCTCTT CCTGGAAGTC ATCCAGCGCC ACAACCACCA GGGTTTTGGA 
GCCGGCAACT TCAACTCACT GTTCAAGGCT TTCGAGGAGG AGCAGAACCT GCGGGGTAAC 
CTCACCAACA TGGAGACCAA TGGGGTGGTG CCCGGCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HPD ( NM_001171993.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165464.1 Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18126
Clone ID Related Accession (Same CDS sequence)	NM_001171993.1
Accession Version	NM_001171993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564761600000
Organism	Homo sapiens(human)
Product	4-hydroxyphenylpyruvate dioxygenase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057510.1 and X72389.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK057510.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162895 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTTG AACCTCTAGC CTACAGGGGC CTGGAGACCG GTTCCCGGGA GGTGGTCAGC 
CATGTAATCA AACAAGGGAA GATTGTGTTT GTCCTCTCCT CAGCGCTCAA CCCCTGGAAC 
AAAGAGATGG GCGATCACCT GGTGAAACAC GGTGACGGAG TGAAGGACAT TGCGTTCGAG 
GTGGAAGATT GTGACTACAT CGTGCAGAAA GCACGGGAAC GGGGCGCCAA AATCATGCGG 
GAGCCCTGGG TAGAGCAAGA CAAGTTTGGG AAGGTGAAGT TTGCTGTGCT GCAGACGTAT 
GGGGACACCA CACACACCCT GGTGGAGAAG ATGAACTACA TCGGCCAATT CTTGCCTGGA 
TATGAGGCCC CAGCGTTCAT GGACCCCCTA CTTCCTAAAC TGCCCAAATG CAGTCTGGAG 
ATGATCGACC ACATTGTGGG AAACCAGCCT GATCAGGAGA TGGTGTCCGC CTCCGAATGG 
TACCTGAAAA ACCTGCAGTT CCACCGCTTC TGGTCCGTGG ATGACACGCA GGTGCACACG 
GAATATAGCT CTCTGCGATC CATTGTGGTG GCCAACTATG AAGAGTCCAT CAAGATGCCC 
ATCAATGAGC CAGCGCCTGG CAAGAAGAAG TCCCAGATCC AGGAATATGT GGACTATAAC 
GGGGGCGCTG GGGTCCAGCA CATCGCTCTC AAGACCGAAG ACATCATCAC AGCGATTCGC 
CACTTGAGAG AGAGAGGCCT GGAGTTCTTA TCTGTTCCCT CCACGTACTA CAAACAACTG 
CGGGAGAAGC TGAAGACGGC CAAGATCAAG GTGAAGGAGA ACATTGATGC CCTGGAGGAG 
CTGAAAATCC TGGTGGACTA CGACGAGAAA GGCTACCTCC TGCAGATCTT CACCAAACCG 
GTGCAGGACC GGCCCACGCT CTTCCTGGAA GTCATCCAGC GCCACAACCA CCAGGGTTTT 
GGAGCCGGCA ACTTCAACTC ACTGTTCAAG GCTTTCGAGG AGGAGCAGAA CCTGCGGGGT 
AACCTCACCA ACATGGAGAC CAATGGGGTG GTGCCCGGCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165464.1
CDS	634..1698
Translation	MGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM

Target ORF information:

RefSeq Version	NM_001171993.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171993.1

ORF Insert Sequence:

ATGGGCTTTG AACCTCTAGC CTACAGGGGC CTGGAGACCG GTTCCCGGGA GGTGGTCAGC 
CATGTAATCA AACAAGGGAA GATTGTGTTT GTCCTCTCCT CAGCGCTCAA CCCCTGGAAC 
AAAGAGATGG GCGATCACCT GGTGAAACAC GGTGACGGAG TGAAGGACAT TGCGTTCGAG 
GTGGAAGATT GTGACTACAT CGTGCAGAAA GCACGGGAAC GGGGCGCCAA AATCATGCGG 
GAGCCCTGGG TAGAGCAAGA CAAGTTTGGG AAGGTGAAGT TTGCTGTGCT GCAGACGTAT 
GGGGACACCA CACACACCCT GGTGGAGAAG ATGAACTACA TCGGCCAATT CTTGCCTGGA 
TATGAGGCCC CAGCGTTCAT GGACCCCCTA CTTCCTAAAC TGCCCAAATG CAGTCTGGAG 
ATGATCGACC ACATTGTGGG AAACCAGCCT GATCAGGAGA TGGTGTCCGC CTCCGAATGG 
TACCTGAAAA ACCTGCAGTT CCACCGCTTC TGGTCCGTGG ATGACACGCA GGTGCACACG 
GAATATAGCT CTCTGCGATC CATTGTGGTG GCCAACTATG AAGAGTCCAT CAAGATGCCC 
ATCAATGAGC CAGCGCCTGG CAAGAAGAAG TCCCAGATCC AGGAATATGT GGACTATAAC 
GGGGGCGCTG GGGTCCAGCA CATCGCTCTC AAGACCGAAG ACATCATCAC AGCGATTCGC 
CACTTGAGAG AGAGAGGCCT GGAGTTCTTA TCTGTTCCCT CCACGTACTA CAAACAACTG 
CGGGAGAAGC TGAAGACGGC CAAGATCAAG GTGAAGGAGA ACATTGATGC CCTGGAGGAG 
CTGAAAATCC TGGTGGACTA CGACGAGAAA GGCTACCTCC TGCAGATCTT CACCAAACCG 
GTGCAGGACC GGCCCACGCT CTTCCTGGAA GTCATCCAGC GCCACAACCA CCAGGGTTTT 
GGAGCCGGCA ACTTCAACTC ACTGTTCAAG GCTTTCGAGG AGGAGCAGAA CCTGCGGGGT 
AACCTCACCA ACATGGAGAC CAATGGGGTG GTGCCCGGCA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The different catalytic roles of the metal-binding ligands in human 4-hydroxyphenylpyruvate dioxygenase.
The Biochemical journal473(9)1179-89(2016 05)
Huang CW,Liu HC,Shen CP,Chen YT,Lee SJ,Lloyd MD,Lee HJ

Hawkinsinuria in two unrelated Greek newborns: identification of a novel variant, biochemical findings and treatment.
Journal of pediatric endocrinology & metabolism : JPEM29(1)15-20(2016 Jan)
Thodi G,Schulpis KH,Dotsikas Y,Pavlides C,Molou E,Chatzidaki M,Triantafylli O,Loukas YL

The interactions in the carboxyl terminus of human 4-hydroxyphenylpyruvate dioxygenase are critical to mediate the conformation of the final helix and the tail to shield the active site for catalysis.
PloS one8(8)e69733(2013)
Lin JF,Sheih YL,Chang TC,Chang NY,Chang CW,Shen CP,Lee HJ

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

A genome-wide association study of metabolic traits in human urine.
Nature genetics43(6)565-9(2011 Jun)
Suhre K,Wallaschofski H,Raffler J,Friedrich N,Haring R,Michael K,Wasner C,Krebs A,Kronenberg F,Chang D,Meisinger C,Wichmann HE,Hoffmann W,Völzke H,Völker U,Teumer A,Biffar R,Kocher T,Felix SB,Illig T,Kroemer HK,Gieger C,Römisch-Margl W,Nauck M

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

HPD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM