GPR132 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPR132
Entrez Gene ID	29933
Full Name	G protein-coupled receptor 132
Synonyms	G2A
General protein information	Preferred Names G protein-coupled receptor 132 Names probable G-protein coupled receptor 132 G2 accumulation protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.33
Summary	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].
Disorder MIM:	606167

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278694.2	NP_001265623.1	probable G-protein coupled receptor 132 isoform 1
NM_001278695.2	NP_001265624.1	probable G-protein coupled receptor 132 isoform 2
NM_013345.4	NP_037477.1	probable G-protein coupled receptor 132 isoform 1
NM_001278695.1	NP_001265624.1	probable G-protein coupled receptor 132 isoform 2
NM_001278694.1	NP_001265623.1	probable G-protein coupled receptor 132 isoform 1
NM_013345.3	NP_037477.1	probable G-protein coupled receptor 132 isoform 1
NM_001278696.2	NP_001265625.1	probable G-protein coupled receptor 132 isoform 3
NM_001278696.1	NP_001265625.1	probable G-protein coupled receptor 132 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR132	NP_037477.1
Mus musculus (house mouse)	Gpr132	NP_064309.1
Danio rerio (zebrafish)	si:ch211-20b12.2	NP_001020718.1
Canis lupus familiaris (dog)	GPR132	XP_005623942.1
Rattus norvegicus (Norway rat)	Gpr132	NP_001164066.1
Danio rerio (zebrafish)	LOC797380	XP_001337849.1
Macaca mulatta (Rhesus monkey)	GPR132	XP_001096948.2
Gallus gallus (chicken)	GPR132	XP_004936473.1
Danio rerio (zebrafish)	LOC101885424	XP_005160531.1
Xenopus tropicalis (tropical clawed frog)	gpr132	NP_001120308.1
Pan troglodytes (chimpanzee)	GPR132	XP_003314586.1
Bos taurus (cattle)	GPR132	XP_002696898.1

Gene Ontology
Bibliography

Related articles in PubMed

Gpr132 sensing of lactate mediates tumor-macrophage interplay to promote breast cancer metastasis.
Chen P, Zuo H, Xiong H, Kolar MJ, Chu Q, Saghatelian A, Siegwart DJ, Wan Y
Proceedings of the National Academy of Sciences of the United States of America114(3)580-585(2017 01)

Heteromerization of G2A and OGR1 enhances proton sensitivity and proton-induced calcium signals.
Huang YH, Su YS, Chang CJ, Sun WH
Journal of receptor and signal transduction research36(6)633-644(2016 Dec)

Direct interaction of Ikaros and Foxp1 modulates expression of the G protein-coupled receptor G2A in B-lymphocytes and acute lymphoblastic leukemia.
Bond J, Domaschenz R, Roman-Trufero M, Sabbattini P, Ferreiros-Vidal I, Gerrard G, Asnafi V, Macintyre E, Merkenschlager M, Dillon N
Oncotarget7(40)65923-65936(2016 10)

Therapeutic options for transfusion related acute lung injury; the potential of the G2A receptor.
Ellison MA, Ambruso DR, Silliman CC
Current pharmaceutical design18(22)3255-9(2012)

Lysophosphatidylcholines activate G2A inducing G(αi)₋₁-/G(αq/)₁₁- Ca²(+) flux, G(βγ)-Hck activation and clathrin/β-arrestin-1/GRK6 recruitment in PMNs.
Khan SY, McLaughlin NJ, Kelher MR, Eckels P, Gamboni-Robertson F, Banerjee A, Silliman CC
The Biochemical journal432(1)35-45(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GPR132 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPR132 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00431	NM_001278695.2 Latest version!	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu04693	NM_001278696.2 Latest version!	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu04694	NM_013345.4 Latest version!	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu04694	NM_001278694.2 Latest version!	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu00431	NM_001278695.1	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu04694	NM_001278694.1	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu04694	NM_013345.3	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu04693	NM_001278696.1	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPR132 ( NM_001278695.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265624.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00431
Clone ID Related Accession (Same CDS sequence)	NM_001278695.2 , NM_001278695.1
Accession Version	NM_001278695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB465600.1 and AA714520.1. On May 31, 2019 this sequence version replaced NM_001278695.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and uses an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB465600.1, AK303654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGGAA ACGCCACCCC AGTGACCACC ACTGCCCCGT GGGCCTCCCT GGGCCTCTCC 
GCCAAGACCT GCAACAACGT GTCCTTCGAA GAGAGCAGGA TAGTCCTGGT CGTGGTGTAC 
AGCGCGGTGT GCACGCTGGG GGTGCCGGCC AACTGCCTGA CTGCGTGGCT GGCGCTGCTG 
CAGGTACTGC AGGGCAACGT GCTGGCCGTC TACCTGCTCT GCCTGGCACT CTGCGAGCTG 
CTGTACACAG GCACGCTGCC ACTCTGGGTC ATCTATATCC GCAACCAGCA CCGCTGGACC 
CTAGGCCTGC TGGCCTGCAA GGTGACCGCC TACATCTTCT TCTGCAACAT CTACGTCAGC 
ATCCTCTTCC TGTGCTGCAT CTCCTGCGAC CGCTTCGTGG CCGTGGTGTA CGCGCTGGAG 
AGTCGGGGCC GCCGCCGCCG GAGGACCGCC ATCCTCATCT CCGCCTGCAT CTTCATCCTC 
GTCGGGATCG TTCACTACCC GGTGTTCCAG ACGGAAGACA AGGAGACCTG CTTTGACATG 
CTGCAGATGG ACAGCAGGAT TGCCGGGTAC TACTACGCCA GGTTCACCGT TGGCTTTGCC 
ATCCCTCTCT CCATCATCGC CTTCACCAAC CACCGGATTT TCAGGAGCAT CAAGCAGAGC 
ATGGGCTTAA GCGCTGCCCA GAAGGCCAAG GTGAAGCACT CGGCCATCGC GGTGGTTGTC 
ATCTTCCTAG TCTGCTTCGC CCCGTACCAC CTGGTTCTCC TCGTCAAAGC CGCTGCCTTT 
TCCTACTACA GAGGAGACAG GAACGCCATG TGCGGCTTGG AGGAAAGGCT GTACACAGCC 
TCTGTGGTGT TTCTGTGCCT GTCCACGGTG AACGGCGTGG CTGACCCCAT TATCTACGTG 
CTGGCCACGG ACCATTCCCG CCAAGAAGTG TCCAGAATCC ATAAGGGGTG GAAAGAGTGG 
TCCATGAAGA CAGACGTCAC CAGGCTCACC CACAGCAGGG ACACCGAGGA GCTGCAGTCG 
CCCGTGGCCC TTGCAGACCA CTACACCTTC TCCAGGCCCG TGCACCCACC AGGGTCACCA 
TGCCCTGCAA AGAGGCTGAT TGAGGAGTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265624.1
CDS	160..1275
Translation	MPGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_001278695.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278695.2

ORF Insert Sequence:

ATGCCAGGAA ACGCCACCCC AGTGACCACC ACTGCCCCGT GGGCCTCCCT GGGCCTCTCC 
GCCAAGACCT GCAACAACGT GTCCTTCGAA GAGAGCAGGA TAGTCCTGGT CGTGGTGTAC 
AGCGCGGTGT GCACGCTGGG GGTGCCGGCC AACTGCCTGA CTGCGTGGCT GGCGCTGCTG 
CAGGTACTGC AGGGCAACGT GCTGGCCGTC TACCTGCTCT GCCTGGCACT CTGCGAGCTG 
CTGTACACAG GCACGCTGCC ACTCTGGGTC ATCTATATCC GCAACCAGCA CCGCTGGACC 
CTAGGCCTGC TGGCCTGCAA GGTGACCGCC TACATCTTCT TCTGCAACAT CTACGTCAGC 
ATCCTCTTCC TGTGCTGCAT CTCCTGCGAC CGCTTCGTGG CCGTGGTGTA CGCGCTGGAG 
AGTCGGGGCC GCCGCCGCCG GAGGACCGCC ATCCTCATCT CCGCCTGCAT CTTCATCCTC 
GTCGGGATCG TTCACTACCC GGTGTTCCAG ACGGAAGACA AGGAGACCTG CTTTGACATG 
CTGCAGATGG ACAGCAGGAT TGCCGGGTAC TACTACGCCA GGTTCACCGT TGGCTTTGCC 
ATCCCTCTCT CCATCATCGC CTTCACCAAC CACCGGATTT TCAGGAGCAT CAAGCAGAGC 
ATGGGCTTAA GCGCTGCCCA GAAGGCCAAG GTGAAGCACT CGGCCATCGC GGTGGTTGTC 
ATCTTCCTAG TCTGCTTCGC CCCGTACCAC CTGGTTCTCC TCGTCAAAGC CGCTGCCTTT 
TCCTACTACA GAGGAGACAG GAACGCCATG TGCGGCTTGG AGGAAAGGCT GTACACAGCC 
TCTGTGGTGT TTCTGTGCCT GTCCACGGTG AACGGCGTGG CTGACCCCAT TATCTACGTG 
CTGGCCACGG ACCATTCCCG CCAAGAAGTG TCCAGAATCC ATAAGGGGTG GAAAGAGTGG 
TCCATGAAGA CAGACGTCAC CAGGCTCACC CACAGCAGGG ACACCGAGGA GCTGCAGTCG 
CCCGTGGCCC TTGCAGACCA CTACACCTTC TCCAGGCCCG TGCACCCACC AGGGTCACCA 
TGCCCTGCAA AGAGGCTGAT TGAGGAGTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR132 ( NM_001278695.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265624.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00431
Clone ID Related Accession (Same CDS sequence)	NM_001278695.2 , NM_001278695.1
Accession Version	NM_001278695.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from W15172.1, AB465600.1 and AA714520.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and uses an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX100740.1, AB465600.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAGGAA ACGCCACCCC AGTGACCACC ACTGCCCCGT GGGCCTCCCT GGGCCTCTCC 
GCCAAGACCT GCAACAACGT GTCCTTCGAA GAGAGCAGGA TAGTCCTGGT CGTGGTGTAC 
AGCGCGGTGT GCACGCTGGG GGTGCCGGCC AACTGCCTGA CTGCGTGGCT GGCGCTGCTG 
CAGGTACTGC AGGGCAACGT GCTGGCCGTC TACCTGCTCT GCCTGGCACT CTGCGAGCTG 
CTGTACACAG GCACGCTGCC ACTCTGGGTC ATCTATATCC GCAACCAGCA CCGCTGGACC 
CTAGGCCTGC TGGCCTGCAA GGTGACCGCC TACATCTTCT TCTGCAACAT CTACGTCAGC 
ATCCTCTTCC TGTGCTGCAT CTCCTGCGAC CGCTTCGTGG CCGTGGTGTA CGCGCTGGAG 
AGTCGGGGCC GCCGCCGCCG GAGGACCGCC ATCCTCATCT CCGCCTGCAT CTTCATCCTC 
GTCGGGATCG TTCACTACCC GGTGTTCCAG ACGGAAGACA AGGAGACCTG CTTTGACATG 
CTGCAGATGG ACAGCAGGAT TGCCGGGTAC TACTACGCCA GGTTCACCGT TGGCTTTGCC 
ATCCCTCTCT CCATCATCGC CTTCACCAAC CACCGGATTT TCAGGAGCAT CAAGCAGAGC 
ATGGGCTTAA GCGCTGCCCA GAAGGCCAAG GTGAAGCACT CGGCCATCGC GGTGGTTGTC 
ATCTTCCTAG TCTGCTTCGC CCCGTACCAC CTGGTTCTCC TCGTCAAAGC CGCTGCCTTT 
TCCTACTACA GAGGAGACAG GAACGCCATG TGCGGCTTGG AGGAAAGGCT GTACACAGCC 
TCTGTGGTGT TTCTGTGCCT GTCCACGGTG AACGGCGTGG CTGACCCCAT TATCTACGTG 
CTGGCCACGG ACCATTCCCG CCAAGAAGTG TCCAGAATCC ATAAGGGGTG GAAAGAGTGG 
TCCATGAAGA CAGACGTCAC CAGGCTCACC CACAGCAGGG ACACCGAGGA GCTGCAGTCG 
CCCGTGGCCC TTGCAGACCA CTACACCTTC TCCAGGCCCG TGCACCCACC AGGGTCACCA 
TGCCCTGCAA AGAGGCTGAT TGAGGAGTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265624.1
CDS	280..1395
Translation	MPGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_001278695.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278695.1

ORF Insert Sequence:

ATGCCAGGAA ACGCCACCCC AGTGACCACC ACTGCCCCGT GGGCCTCCCT GGGCCTCTCC 
GCCAAGACCT GCAACAACGT GTCCTTCGAA GAGAGCAGGA TAGTCCTGGT CGTGGTGTAC 
AGCGCGGTGT GCACGCTGGG GGTGCCGGCC AACTGCCTGA CTGCGTGGCT GGCGCTGCTG 
CAGGTACTGC AGGGCAACGT GCTGGCCGTC TACCTGCTCT GCCTGGCACT CTGCGAGCTG 
CTGTACACAG GCACGCTGCC ACTCTGGGTC ATCTATATCC GCAACCAGCA CCGCTGGACC 
CTAGGCCTGC TGGCCTGCAA GGTGACCGCC TACATCTTCT TCTGCAACAT CTACGTCAGC 
ATCCTCTTCC TGTGCTGCAT CTCCTGCGAC CGCTTCGTGG CCGTGGTGTA CGCGCTGGAG 
AGTCGGGGCC GCCGCCGCCG GAGGACCGCC ATCCTCATCT CCGCCTGCAT CTTCATCCTC 
GTCGGGATCG TTCACTACCC GGTGTTCCAG ACGGAAGACA AGGAGACCTG CTTTGACATG 
CTGCAGATGG ACAGCAGGAT TGCCGGGTAC TACTACGCCA GGTTCACCGT TGGCTTTGCC 
ATCCCTCTCT CCATCATCGC CTTCACCAAC CACCGGATTT TCAGGAGCAT CAAGCAGAGC 
ATGGGCTTAA GCGCTGCCCA GAAGGCCAAG GTGAAGCACT CGGCCATCGC GGTGGTTGTC 
ATCTTCCTAG TCTGCTTCGC CCCGTACCAC CTGGTTCTCC TCGTCAAAGC CGCTGCCTTT 
TCCTACTACA GAGGAGACAG GAACGCCATG TGCGGCTTGG AGGAAAGGCT GTACACAGCC 
TCTGTGGTGT TTCTGTGCCT GTCCACGGTG AACGGCGTGG CTGACCCCAT TATCTACGTG 
CTGGCCACGG ACCATTCCCG CCAAGAAGTG TCCAGAATCC ATAAGGGGTG GAAAGAGTGG 
TCCATGAAGA CAGACGTCAC CAGGCTCACC CACAGCAGGG ACACCGAGGA GCTGCAGTCG 
CCCGTGGCCC TTGCAGACCA CTACACCTTC TCCAGGCCCG TGCACCCACC AGGGTCACCA 
TGCCCTGCAA AGAGGCTGAT TGAGGAGTCC TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR132 ( NM_001278696.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265625.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu04693
Clone ID Related Accession (Same CDS sequence)	NM_001278696.2 , NM_001278696.1
Accession Version	NM_001278696.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB137292.1 and BC084546.1. On May 31, 2019 this sequence version replaced NM_001278696.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGA TGGACAGCAG GATTGCCGGG TACTACTACG CCAGGTTCAC CGTTGGCTTT 
GCCATCCCTC TCTCCATCAT CGCCTTCACC AACCACCGGA TTTTCAGGAG CATCAAGCAG 
AGCATGGGCT TAAGCGCTGC CCAGAAGGCC AAGGTGAAGC ACTCGGCCAT CGCGGTGGTT 
GTCATCTTCC TAGTCTGCTT CGCCCCGTAC CACCTGGTTC TCCTCGTCAA AGCCGCTGCC 
TTTTCCTACT ACAGAGGAGA CAGGAACGCC ATGTGCGGCT TGGAGGAAAG GCTGTACACA 
GCCTCTGTGG TGTTTCTGTG CCTGTCCACG GTGAACGGCG TGGCTGACCC CATTATCTAC 
GTGCTGGCCA CGGACCATTC CCGCCAAGAA GTGTCCAGAA TCCATAAGGG GTGGAAAGAG 
TGGTCCATGA AGACAGACGT CACCAGGCTC ACCCACAGCA GGGACACCGA GGAGCTGCAG 
TCGCCCGTGG CCCTTGCAGA CCACTACACC TTCTCCAGGC CCGTGCACCC ACCAGGGTCA 
CCATGCCCTG CAAAGAGGCT GATTGAGGAG TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265625.1
CDS	583..1161
Translation	MLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_001278696.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278696.2

ORF Insert Sequence:

ATGCTGCAGA TGGACAGCAG GATTGCCGGG TACTACTACG CCAGGTTCAC CGTTGGCTTT 
GCCATCCCTC TCTCCATCAT CGCCTTCACC AACCACCGGA TTTTCAGGAG CATCAAGCAG 
AGCATGGGCT TAAGCGCTGC CCAGAAGGCC AAGGTGAAGC ACTCGGCCAT CGCGGTGGTT 
GTCATCTTCC TAGTCTGCTT CGCCCCGTAC CACCTGGTTC TCCTCGTCAA AGCCGCTGCC 
TTTTCCTACT ACAGAGGAGA CAGGAACGCC ATGTGCGGCT TGGAGGAAAG GCTGTACACA 
GCCTCTGTGG TGTTTCTGTG CCTGTCCACG GTGAACGGCG TGGCTGACCC CATTATCTAC 
GTGCTGGCCA CGGACCATTC CCGCCAAGAA GTGTCCAGAA TCCATAAGGG GTGGAAAGAG 
TGGTCCATGA AGACAGACGT CACCAGGCTC ACCCACAGCA GGGACACCGA GGAGCTGCAG 
TCGCCCGTGG CCCTTGCAGA CCACTACACC TTCTCCAGGC CCGTGCACCC ACCAGGGTCA 
CCATGCCCTG CAAAGAGGCT GATTGAGGAG TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR132 ( NM_001278696.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265625.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu04693
Clone ID Related Accession (Same CDS sequence)	NM_001278696.2 , NM_001278696.1
Accession Version	NM_001278696.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from W15172.1, DB137292.1, BC084546.1 and AA714520.1. Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB137292.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGA TGGACAGCAG GATTGCCGGG TACTACTACG CCAGGTTCAC CGTTGGCTTT 
GCCATCCCTC TCTCCATCAT CGCCTTCACC AACCACCGGA TTTTCAGGAG CATCAAGCAG 
AGCATGGGCT TAAGCGCTGC CCAGAAGGCC AAGGTGAAGC ACTCGGCCAT CGCGGTGGTT 
GTCATCTTCC TAGTCTGCTT CGCCCCGTAC CACCTGGTTC TCCTCGTCAA AGCCGCTGCC 
TTTTCCTACT ACAGAGGAGA CAGGAACGCC ATGTGCGGCT TGGAGGAAAG GCTGTACACA 
GCCTCTGTGG TGTTTCTGTG CCTGTCCACG GTGAACGGCG TGGCTGACCC CATTATCTAC 
GTGCTGGCCA CGGACCATTC CCGCCAAGAA GTGTCCAGAA TCCATAAGGG GTGGAAAGAG 
TGGTCCATGA AGACAGACGT CACCAGGCTC ACCCACAGCA GGGACACCGA GGAGCTGCAG 
TCGCCCGTGG CCCTTGCAGA CCACTACACC TTCTCCAGGC CCGTGCACCC ACCAGGGTCA 
CCATGCCCTG CAAAGAGGCT GATTGAGGAG TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265625.1
CDS	703..1281
Translation	MLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_001278696.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278696.1

ORF Insert Sequence:

ATGCTGCAGA TGGACAGCAG GATTGCCGGG TACTACTACG CCAGGTTCAC CGTTGGCTTT 
GCCATCCCTC TCTCCATCAT CGCCTTCACC AACCACCGGA TTTTCAGGAG CATCAAGCAG 
AGCATGGGCT TAAGCGCTGC CCAGAAGGCC AAGGTGAAGC ACTCGGCCAT CGCGGTGGTT 
GTCATCTTCC TAGTCTGCTT CGCCCCGTAC CACCTGGTTC TCCTCGTCAA AGCCGCTGCC 
TTTTCCTACT ACAGAGGAGA CAGGAACGCC ATGTGCGGCT TGGAGGAAAG GCTGTACACA 
GCCTCTGTGG TGTTTCTGTG CCTGTCCACG GTGAACGGCG TGGCTGACCC CATTATCTAC 
GTGCTGGCCA CGGACCATTC CCGCCAAGAA GTGTCCAGAA TCCATAAGGG GTGGAAAGAG 
TGGTCCATGA AGACAGACGT CACCAGGCTC ACCCACAGCA GGGACACCGA GGAGCTGCAG 
TCGCCCGTGG CCCTTGCAGA CCACTACACC TTCTCCAGGC CCGTGCACCC ACCAGGGTCA 
CCATGCCCTG CAAAGAGGCT GATTGAGGAG TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR132 ( NM_001278694.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265623.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 1, mRNA.

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CloneID	OHu04694
Clone ID Related Accession (Same CDS sequence)	NM_001278694.2 , NM_013345.4 , NM_001278694.1 , NM_013345.3
Accession Version	NM_001278694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292142.1 and BC084546.1. On May 31, 2019 this sequence version replaced NM_001278694.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292142.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265623.1
CDS	1003..2145
Translation	MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_001278694.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278694.2

ORF Insert Sequence:

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR132 ( NM_013345.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037477.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04694
Clone ID Related Accession (Same CDS sequence)	NM_001278694.2 , NM_013345.4 , NM_001278694.1 , NM_013345.3
Accession Version	NM_013345.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB465599.1 and AA714520.1. On Nov 23, 2018 this sequence version replaced NM_013345.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB465599.1, AF083955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329797.8/ ENSP00000328818.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037477.1
CDS	913..2055
Translation	MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_013345.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013345.4

ORF Insert Sequence:

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR132 ( NM_001278694.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265623.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04694
Clone ID Related Accession (Same CDS sequence)	NM_001278694.2 , NM_013345.4 , NM_001278694.1 , NM_013345.3
Accession Version	NM_001278694.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from W15172.1, AK292142.1, BC084546.1 and AA714520.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292142.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265623.1
CDS	1123..2265
Translation	MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_001278694.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278694.1

ORF Insert Sequence:

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR132 ( NM_013345.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037477.1 Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04694
Clone ID Related Accession (Same CDS sequence)	NM_001278694.2 , NM_013345.4 , NM_001278694.1 , NM_013345.3
Accession Version	NM_013345.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 132 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from W15172.1, AB465599.1 and AA714520.1. On Jul 17, 2013 this sequence version replaced NM_013345.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB465599.1, AF083955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037477.1
CDS	1033..2175
Translation	MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC

Target ORF information:

RefSeq Version	NM_013345.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 132 (GPR132), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013345.3

ORF Insert Sequence:

ATGTGCCCAA TGCTACTGAA AAACGGTTAC AATGGAAACG CCACCCCAGT GACCACCACT 
GCCCCGTGGG CCTCCCTGGG CCTCTCCGCC AAGACCTGCA ACAACGTGTC CTTCGAAGAG 
AGCAGGATAG TCCTGGTCGT GGTGTACAGC GCGGTGTGCA CGCTGGGGGT GCCGGCCAAC 
TGCCTGACTG CGTGGCTGGC GCTGCTGCAG GTACTGCAGG GCAACGTGCT GGCCGTCTAC 
CTGCTCTGCC TGGCACTCTG CGAGCTGCTG TACACAGGCA CGCTGCCACT CTGGGTCATC 
TATATCCGCA ACCAGCACCG CTGGACCCTA GGCCTGCTGG CCTGCAAGGT GACCGCCTAC 
ATCTTCTTCT GCAACATCTA CGTCAGCATC CTCTTCCTGT GCTGCATCTC CTGCGACCGC 
TTCGTGGCCG TGGTGTACGC GCTGGAGAGT CGGGGCCGCC GCCGCCGGAG GACCGCCATC 
CTCATCTCCG CCTGCATCTT CATCCTCGTC GGGATCGTTC ACTACCCGGT GTTCCAGACG 
GAAGACAAGG AGACCTGCTT TGACATGCTG CAGATGGACA GCAGGATTGC CGGGTACTAC 
TACGCCAGGT TCACCGTTGG CTTTGCCATC CCTCTCTCCA TCATCGCCTT CACCAACCAC 
CGGATTTTCA GGAGCATCAA GCAGAGCATG GGCTTAAGCG CTGCCCAGAA GGCCAAGGTG 
AAGCACTCGG CCATCGCGGT GGTTGTCATC TTCCTAGTCT GCTTCGCCCC GTACCACCTG 
GTTCTCCTCG TCAAAGCCGC TGCCTTTTCC TACTACAGAG GAGACAGGAA CGCCATGTGC 
GGCTTGGAGG AAAGGCTGTA CACAGCCTCT GTGGTGTTTC TGTGCCTGTC CACGGTGAAC 
GGCGTGGCTG ACCCCATTAT CTACGTGCTG GCCACGGACC ATTCCCGCCA AGAAGTGTCC 
AGAATCCATA AGGGGTGGAA AGAGTGGTCC ATGAAGACAG ACGTCACCAG GCTCACCCAC 
AGCAGGGACA CCGAGGAGCT GCAGTCGCCC GTGGCCCTTG CAGACCACTA CACCTTCTCC 
AGGCCCGTGC ACCCACCAGG GTCACCATGC CCTGCAAAGA GGCTGATTGA GGAGTCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gpr132 sensing of lactate mediates tumor-macrophage interplay to promote breast cancer metastasis.
Proceedings of the National Academy of Sciences of the United States of America114(3)580-585(2017 01)
Chen P,Zuo H,Xiong H,Kolar MJ,Chu Q,Saghatelian A,Siegwart DJ,Wan Y

Heteromerization of G2A and OGR1 enhances proton sensitivity and proton-induced calcium signals.
Journal of receptor and signal transduction research36(6)633-644(2016 Dec)
Huang YH,Su YS,Chang CJ,Sun WH

Direct interaction of Ikaros and Foxp1 modulates expression of the G protein-coupled receptor G2A in B-lymphocytes and acute lymphoblastic leukemia.
Oncotarget7(40)65923-65936(2016 10)
Bond J,Domaschenz R,Roman-Trufero M,Sabbattini P,Ferreiros-Vidal I,Gerrard G,Asnafi V,Macintyre E,Merkenschlager M,Dillon N

Therapeutic options for transfusion related acute lung injury; the potential of the G2A receptor.
Current pharmaceutical design18(22)3255-9(2012)
Ellison MA,Ambruso DR,Silliman CC

Lysophosphatidylcholines activate G2A inducing G(αi)₋₁-/G(αq/)₁₁- Ca²(+) flux, G(βγ)-Hck activation and clathrin/β-arrestin-1/GRK6 recruitment in PMNs.
The Biochemical journal432(1)35-45(2010 Nov)
Khan SY,McLaughlin NJ,Kelher MR,Eckels P,Gamboni-Robertson F,Banerjee A,Silliman CC

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GPR132 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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