EEF2K cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF2K
Entrez Gene ID	29904
Full Name	eukaryotic elongation factor 2 kinase
Synonyms	HSU93850,eEF-2K
General protein information	Preferred Names eukaryotic elongation factor 2 kinase Names eukaryotic elongation factor 2 kinase calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase calmodulin-dependent protein kinase III eEF-2 kinase elongation factor-2 kinase eukaroytic elongation factor 2 kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p12.2
Summary	This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008].
Disorder MIM:	606968

mRNA and Protein(s)

mRNA	Protein	Name
NM_013302.4	NP_037434.1	eukaryotic elongation factor 2 kinase
NM_013302.3	NP_037434.1	eukaryotic elongation factor 2 kinase
XM_017023193.2	XP_016878682.1	eukaryotic elongation factor 2 kinase isoform X1
XM_017023194.2	XP_016878683.1	eukaryotic elongation factor 2 kinase isoform X2
XM_017023193.1	XP_016878682.1	eukaryotic elongation factor 2 kinase isoform X1
XM_017023194.1	XP_016878683.1	eukaryotic elongation factor 2 kinase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EEF2K	NP_037434.1
Bos taurus (cattle)	EEF2K	NP_001179471.1
Macaca mulatta (Rhesus monkey)	EEF2K	XP_001092565.1
Canis lupus familiaris (dog)	EEF2K	XP_005621491.1
Pan troglodytes (chimpanzee)	EEF2K	XP_001161085.2
Rattus norvegicus (Norway rat)	Eef2k	NP_037079.1
Danio rerio (zebrafish)	eef2k	NP_001002740.1
Caenorhabditis elegans (roundworm)	efk-1	NP_001022572.1
Xenopus tropicalis (tropical clawed frog)	eef2k	NP_001120146.1
Mus musculus (house mouse)	Eef2k	NP_001254640.1
Gallus gallus (chicken)	EEF2K	XP_004945358.1

Gene Ontology
Bibliography

Related articles in PubMed

Interplay between reversible phosphorylation and irreversible ADP-ribosylation of eukaryotic translation elongation factor 2.
Mateus-Seidl R, Stahl S, Dengl S, Birzele F, Herrmuth H, Mayer K, Niederfellner G, Liu XF, Pastan I, Brinkmann U
Biological chemistry400(4)501-512(2019 03)

Genetic reduction of eEF2 kinase alleviates pathophysiology in Alzheimer's disease model mice.
Beckelman BC, Yang W, Kasica NP, Zimmermann HR, Zhou X, Keene CD, Ryazanov AG, Ma T
The Journal of clinical investigation129(2)820-833(2019 02)

Thymoquinone inhibits cell proliferation, migration, and invasion by regulating the elongation factor 2 kinase (eEF-2K) signaling axis in triple-negative breast cancer.
Kabil N, Bayraktar R, Kahraman N, Mokhlis HA, Calin GA, Lopez-Berestein G, Ozpolat B
Breast cancer research and treatment171(3)593-605(2018 Oct)

Elongation factor-2 kinase (eEF-2K) expression is associated with poor patient survival and promotes proliferation, invasion and tumor growth of lung cancer.
Bircan HA, Gurbuz N, Pataer A, Caner A, Kahraman N, Bayraktar E, Bayraktar R, Erdogan MA, Kabil N, Ozpolat B
Lung cancer (Amsterdam, Netherlands)12431-39(2018 10)

Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity.
Jan A, Jansonius B, Delaidelli A, Bhanshali F, An YA, Ferreira N, Smits LM, Negri GL, Schwamborn JC, Jensen PH, Mackenzie IR, Taubert S, Sorensen PH
Acta neuropathologica communications6(1)54(2018 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EEF2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu86007	XM_017023193.2 Latest version!	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu86008	XM_017023194.2 Latest version!	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu13523	NM_013302.4 Latest version!	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00
View Old Accession Versions >>
OHu13523	NM_013302.3	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00
OHu86007	XM_017023193.1	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu86008	XM_017023194.1	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF2K ( XM_017023193.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878682.1 Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

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CloneID	OHu86007
Clone ID Related Accession (Same CDS sequence)	XM_017023193.2 , XM_017023193.1
Accession Version	XM_017023193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	eukaryotic elongation factor 2 kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878682.1
CDS	197..2374
Translation	MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_017023193.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023193.2

ORF Insert Sequence:

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2K ( XM_017023193.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878682.1 Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86007
Clone ID Related Accession (Same CDS sequence)	XM_017023193.2 , XM_017023193.1
Accession Version	XM_017023193.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	eukaryotic elongation factor 2 kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878682.1
CDS	197..2374
Translation	MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_017023193.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023193.1

ORF Insert Sequence:

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2K ( XM_017023194.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878683.1 Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

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CloneID	OHu86008
Clone ID Related Accession (Same CDS sequence)	XM_017023194.2 , XM_017023194.1
Accession Version	XM_017023194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	eukaryotic elongation factor 2 kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGTA 
TGTGTAGAGA AGTGGAATCT CCTCAACTCC TCCCGCCTCC ACCTGCCGAG GGCTTCGGCC 
GTGGCCCTGG AAGTGCAAAG GCTTAATGCT CTGGACCTCG AAAAGAAAAT CGGGAAGTCC 
ATTTTGGGGA AGGTCCATCT GGCCATGGTG CGCTACCACG AGGGTGGGCG CTTCTGCGAG 
AAGGGCGAGG AGTGGGACCA GGAGTCGGCT GTCTTCCACC TGGAGCACGC AGCCAACCTG 
GGCGAGCTGG AGGCCATCGT GGGCCTGGGA CTCATGTACT CGCAGTTGCC TCATCACATC 
CTAGCCGATG TCTCTCTGAA GGAGACAGAA GAGAACAAAA CCAAAGGATT TGATTACTTA 
CTAAAGGCCG CTGAAGCTGG CGACAGGCAG TCCATGATCC TAGTGGCGCG AGCTTTTGAC 
TCTGGCCAGA ACCTCAGCCC GGACAGGTGC CAAGACTGGC TAGAGGCCCT GCACTGGTAC 
AACACTGCCC TGGAGATGAC GGACTGTGAT GAGGGCGGTG AGTACGACGG AATGCAGGAC 
GAGCCCCGGT ACATGATGCT GGCCAGGGAG GCCGAGATGC TGTTCACAGG AGGCTACGGG 
CTGGAGAAGG ACCCGCAGAG ATCAGGGGAC TTGTATACCC AGGCAGCAGA GGCAGCGATG 
GAAGCCATGA AGGGCCGACT GGCCAACCAG TACTACCAAA AGGCTGAAGA GGCCTGGGCC 
CAGATGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878683.1
CDS	475..2589
Translation	MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_017023194.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023194.2

ORF Insert Sequence:

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGTA 
TGTGTAGAGA AGTGGAATCT CCTCAACTCC TCCCGCCTCC ACCTGCCGAG GGCTTCGGCC 
GTGGCCCTGG AAGTGCAAAG GCTTAATGCT CTGGACCTCG AAAAGAAAAT CGGGAAGTCC 
ATTTTGGGGA AGGTCCATCT GGCCATGGTG CGCTACCACG AGGGTGGGCG CTTCTGCGAG 
AAGGGCGAGG AGTGGGACCA GGAGTCGGCT GTCTTCCACC TGGAGCACGC AGCCAACCTG 
GGCGAGCTGG AGGCCATCGT GGGCCTGGGA CTCATGTACT CGCAGTTGCC TCATCACATC 
CTAGCCGATG TCTCTCTGAA GGAGACAGAA GAGAACAAAA CCAAAGGATT TGATTACTTA 
CTAAAGGCCG CTGAAGCTGG CGACAGGCAG TCCATGATCC TAGTGGCGCG AGCTTTTGAC 
TCTGGCCAGA ACCTCAGCCC GGACAGGTGC CAAGACTGGC TAGAGGCCCT GCACTGGTAC 
AACACTGCCC TGGAGATGAC GGACTGTGAT GAGGGCGGTG AGTACGACGG AATGCAGGAC 
GAGCCCCGGT ACATGATGCT GGCCAGGGAG GCCGAGATGC TGTTCACAGG AGGCTACGGG 
CTGGAGAAGG ACCCGCAGAG ATCAGGGGAC TTGTATACCC AGGCAGCAGA GGCAGCGATG 
GAAGCCATGA AGGGCCGACT GGCCAACCAG TACTACCAAA AGGCTGAAGA GGCCTGGGCC 
CAGATGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2K ( XM_017023194.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878683.1 Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu86008
Clone ID Related Accession (Same CDS sequence)	XM_017023194.2 , XM_017023194.1
Accession Version	XM_017023194.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	eukaryotic elongation factor 2 kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGTA 
TGTGTAGAGA AGTGGAATCT CCTCAACTCC TCCCGCCTCC ACCTGCCGAG GGCTTCGGCC 
GTGGCCCTGG AAGTGCAAAG GCTTAATGCT CTGGACCTCG AAAAGAAAAT CGGGAAGTCC 
ATTTTGGGGA AGGTCCATCT GGCCATGGTG CGCTACCACG AGGGTGGGCG CTTCTGCGAG 
AAGGGCGAGG AGTGGGACCA GGAGTCGGCT GTCTTCCACC TGGAGCACGC AGCCAACCTG 
GGCGAGCTGG AGGCCATCGT GGGCCTGGGA CTCATGTACT CGCAGTTGCC TCATCACATC 
CTAGCCGATG TCTCTCTGAA GGAGACAGAA GAGAACAAAA CCAAAGGATT TGATTACTTA 
CTAAAGGCCG CTGAAGCTGG CGACAGGCAG TCCATGATCC TAGTGGCGCG AGCTTTTGAC 
TCTGGCCAGA ACCTCAGCCC GGACAGGTGC CAAGACTGGC TAGAGGCCCT GCACTGGTAC 
AACACTGCCC TGGAGATGAC GGACTGTGAT GAGGGCGGTG AGTACGACGG AATGCAGGAC 
GAGCCCCGGT ACATGATGCT GGCCAGGGAG GCCGAGATGC TGTTCACAGG AGGCTACGGG 
CTGGAGAAGG ACCCGCAGAG ATCAGGGGAC TTGTATACCC AGGCAGCAGA GGCAGCGATG 
GAAGCCATGA AGGGCCGACT GGCCAACCAG TACTACCAAA AGGCTGAAGA GGCCTGGGCC 
CAGATGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878683.1
CDS	493..2607
Translation	MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_017023194.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023194.1

ORF Insert Sequence:

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGTA 
TGTGTAGAGA AGTGGAATCT CCTCAACTCC TCCCGCCTCC ACCTGCCGAG GGCTTCGGCC 
GTGGCCCTGG AAGTGCAAAG GCTTAATGCT CTGGACCTCG AAAAGAAAAT CGGGAAGTCC 
ATTTTGGGGA AGGTCCATCT GGCCATGGTG CGCTACCACG AGGGTGGGCG CTTCTGCGAG 
AAGGGCGAGG AGTGGGACCA GGAGTCGGCT GTCTTCCACC TGGAGCACGC AGCCAACCTG 
GGCGAGCTGG AGGCCATCGT GGGCCTGGGA CTCATGTACT CGCAGTTGCC TCATCACATC 
CTAGCCGATG TCTCTCTGAA GGAGACAGAA GAGAACAAAA CCAAAGGATT TGATTACTTA 
CTAAAGGCCG CTGAAGCTGG CGACAGGCAG TCCATGATCC TAGTGGCGCG AGCTTTTGAC 
TCTGGCCAGA ACCTCAGCCC GGACAGGTGC CAAGACTGGC TAGAGGCCCT GCACTGGTAC 
AACACTGCCC TGGAGATGAC GGACTGTGAT GAGGGCGGTG AGTACGACGG AATGCAGGAC 
GAGCCCCGGT ACATGATGCT GGCCAGGGAG GCCGAGATGC TGTTCACAGG AGGCTACGGG 
CTGGAGAAGG ACCCGCAGAG ATCAGGGGAC TTGTATACCC AGGCAGCAGA GGCAGCGATG 
GAAGCCATGA AGGGCCGACT GGCCAACCAG TACTACCAAA AGGCTGAAGA GGCCTGGGCC 
CAGATGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2K ( NM_013302.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037434.1 Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

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CloneID	OHu13523
Clone ID Related Accession (Same CDS sequence)	NM_013302.4 , NM_013302.3
Accession Version	NM_013302.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Homo sapiens(human)
Product	eukaryotic elongation factor 2 kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA735877.1, DA556486.1, U93850.1, CA397138.1, AW675420.1 and AC009034.10. On Nov 23, 2018 this sequence version replaced NM_013302.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC032665.1, U93850.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037434.1
CDS	479..2656
Translation	MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPRVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	NM_013302.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013302.4

ORF Insert Sequence:

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2K ( NM_013302.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037434.1 Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

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CloneID	OHu13523
Clone ID Related Accession (Same CDS sequence)	NM_013302.4 , NM_013302.3
Accession Version	NM_013302.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512403200000
Organism	Homo sapiens(human)
Product	eukaryotic elongation factor 2 kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA735877.1, DA556486.1, U93850.1, CA397138.1, AW675420.1 and AC009034.10. On Mar 16, 2006 this sequence version replaced NM_013302.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037434.1
CDS	486..2663
Translation	MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPRVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	NM_013302.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013302.3

ORF Insert Sequence:

ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC 
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC 
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC 
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC 
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA 
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG 
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG 
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC 
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC 
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT 
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA 
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC 
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC 
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT 
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC 
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC 
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC 
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG 
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG 
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG 
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC 
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC 
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC 
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC 
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG 
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG 
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC 
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG 
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG 
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interplay between reversible phosphorylation and irreversible ADP-ribosylation of eukaryotic translation elongation factor 2.
Biological chemistry400(4)501-512(2019 03)
Mateus-Seidl R,Stahl S,Dengl S,Birzele F,Herrmuth H,Mayer K,Niederfellner G,Liu XF,Pastan I,Brinkmann U

Genetic reduction of eEF2 kinase alleviates pathophysiology in Alzheimer's disease model mice.
The Journal of clinical investigation129(2)820-833(2019 02)
Beckelman BC,Yang W,Kasica NP,Zimmermann HR,Zhou X,Keene CD,Ryazanov AG,Ma T

Thymoquinone inhibits cell proliferation, migration, and invasion by regulating the elongation factor 2 kinase (eEF-2K) signaling axis in triple-negative breast cancer.
Breast cancer research and treatment171(3)593-605(2018 Oct)
Kabil N,Bayraktar R,Kahraman N,Mokhlis HA,Calin GA,Lopez-Berestein G,Ozpolat B

Elongation factor-2 kinase (eEF-2K) expression is associated with poor patient survival and promotes proliferation, invasion and tumor growth of lung cancer.
Lung cancer (Amsterdam, Netherlands)12431-39(2018 10)
Bircan HA,Gurbuz N,Pataer A,Caner A,Kahraman N,Bayraktar E,Bayraktar R,Erdogan MA,Kabil N,Ozpolat B

Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity.
Acta neuropathologica communications6(1)54(2018 07)
Jan A,Jansonius B,Delaidelli A,Bhanshali F,An YA,Ferreira N,Smits LM,Negri GL,Schwamborn JC,Jensen PH,Mackenzie IR,Taubert S,Sorensen PH

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EEF2K cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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