GPR4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPR4
Entrez Gene ID	2828
Full Name	G protein-coupled receptor 4
General protein information	Preferred Names G protein-coupled receptor 4 Names G-protein coupled receptor 4 G-protein coupled receptor 19
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.32
Disorder MIM:	600551

mRNA and Protein(s)

mRNA	Protein	Name
NM_005282.2	NP_005273.1	G-protein coupled receptor 4
XM_017026608.1	XP_016882097.1	G-protein coupled receptor 4 isoform X1
XM_017026607.1	XP_016882096.1	G-protein coupled receptor 4 isoform X1
XM_017026607.2	XP_016882096.1	G-protein coupled receptor 4 isoform X1
NM_005282.3	NP_005273.1	G-protein coupled receptor 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPR4	NP_005273.1
Macaca mulatta (Rhesus monkey)	LOC716148	XP_001106715.2
Canis lupus familiaris (dog)	GPR4	XP_005616498.1
Mus musculus (house mouse)	Gpr4	NP_783599.1
Bos taurus (cattle)	GPR4	NP_001069051.1
Rattus norvegicus (Norway rat)	Gpr4	NP_001020851.1
Danio rerio (zebrafish)	LOC563762	XP_005157731.1

Gene Ontology
Bibliography

Related articles in PubMed

CNS distribution, signalling properties and central effects of G-protein coupled receptor 4.
Hosford PS, Mosienko V, Kishi K, Jurisic G, Seuwen K, Kinzel B, Ludwig MG, Wells JA, Christie IN, Koolen L, Abdala AP, Liu BH, Gourine AV, Teschemacher AG, Kasparov S
Neuropharmacology138381-392(2018 08)

GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOP.
Dong B, Zhang X, Fan Y, Cao S, Zhang X
The Biochemical journal474(24)4065-4074(2017 12)

Manganese and cobalt activate zebrafish ovarian cancer G-protein-coupled receptor 1 but not GPR4.
Negishi J, Omori Y, Shindo M, Takanashi H, Musha S, Nagayama S, Hirayama J, Nishina H, Nakakura T, Mogi C, Sato K, Okajima F, Mochimaru Y, Tomura H
Journal of receptor and signal transduction research37(4)401-408(2017 Aug)

Acidosis Activates Endoplasmic Reticulum Stress Pathways through GPR4 in Human Vascular Endothelial Cells.
Dong L, Krewson EA, Yang LV
International journal of molecular sciences18(2)(2017 Jan)

Human GPR4 and the Notch signaling pathway in endothelial cell tube formation.
Ren J, Zhang Y, Cai H, Ma H, Zhao D, Zhang X, Li Z, Wang S, Wang J, Liu R, Li Y, Qian J, Wei H, Niu L, Liu Y, Xiao L, Ding M, Jiang S
Molecular medicine reports14(2)1235-40(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GPR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31858	NM_005282.3 Latest version!	Homo sapiens G protein-coupled receptor 4 (GPR4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu31858	XM_017026607.2 Latest version!	Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu31858	XM_017026608.1 Latest version!	Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu31858	XM_017026607.1	Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu31858	NM_005282.2	Homo sapiens G protein-coupled receptor 4 (GPR4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPR4 ( NM_005282.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005273.1 Homo sapiens G protein-coupled receptor 4 (GPR4), mRNA.

Price & Availability↑

CloneID	OHu31858
Clone ID Related Accession (Same CDS sequence)	NM_005282.2 , XM_017026608.1 , XM_017026607.1 , XM_017026607.2 , NM_005282.3
Accession Version	NM_005282.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512835200000
Organism	Homo sapiens(human)
Product	G-protein coupled receptor 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA858082.1, BC038450.1 and BE669939.1. On Oct 21, 2008 this sequence version replaced NM_005282.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.214748.1, BC038450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005273.1
CDS	946..2034
Translation	MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

Target ORF information:

RefSeq Version	NM_005282.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 4 (GPR4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005282.2

ORF Insert Sequence:

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR4 ( NM_005282.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005273.1 Homo sapiens G protein-coupled receptor 4 (GPR4), mRNA.

Price & Availability↑

CloneID	OHu31858
Clone ID Related Accession (Same CDS sequence)	NM_005282.2 , XM_017026608.1 , XM_017026607.1 , XM_017026607.2 , NM_005282.3
Accession Version	NM_005282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	G-protein coupled receptor 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011480.3, DA858082.1, BC038450.1 and BE669939.1. On Nov 23, 2018 this sequence version replaced NM_005282.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038450.1, SRR1660809.224954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000323040.5/ ENSP00000319744.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005273.1
CDS	950..2038
Translation	MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

Target ORF information:

RefSeq Version	NM_005282.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 4 (GPR4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005282.3

ORF Insert Sequence:

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR4 ( XM_017026608.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882097.1 Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu31858
Clone ID Related Accession (Same CDS sequence)	NM_005282.2 , XM_017026608.1 , XM_017026607.1 , XM_017026607.2 , NM_005282.3
Accession Version	XM_017026608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	G-protein coupled receptor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882097.1
CDS	855..1943
Translation	MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

Target ORF information:

RefSeq Version	XM_017026608.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026608.1

ORF Insert Sequence:

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR4 ( XM_017026607.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882096.1 Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu31858
Clone ID Related Accession (Same CDS sequence)	NM_005282.2 , XM_017026608.1 , XM_017026607.1 , XM_017026607.2 , NM_005282.3
Accession Version	XM_017026607.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	G-protein coupled receptor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882096.1
CDS	1534..2622
Translation	MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

Target ORF information:

RefSeq Version	XM_017026607.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026607.1

ORF Insert Sequence:

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR4 ( XM_017026607.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882096.1 Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X1, mRNA.

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CloneID	OHu31858
Clone ID Related Accession (Same CDS sequence)	NM_005282.2 , XM_017026608.1 , XM_017026607.1 , XM_017026607.2 , NM_005282.3
Accession Version	XM_017026607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	G-protein coupled receptor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017026607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882096.1
CDS	2110..3198
Translation	MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

Target ORF information:

RefSeq Version	XM_017026607.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 4 (GPR4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026607.2

ORF Insert Sequence:

ATGGGCAACC ACACGTGGGA GGGCTGCCAC GTGGACTCGC GCGTGGACCA CCTCTTTCCG 
CCATCCCTCT ACATCTTTGT CATCGGCGTG GGGCTGCCCA CCAACTGCCT GGCTCTGTGG 
GCGGCCTACC GCCAGGTGCA ACAGCGCAAC GAGCTGGGCG TCTACCTGAT GAACCTCAGC 
ATCGCCGACC TGCTGTACAT CTGCACGCTG CCGCTGTGGG TGGACTACTT CCTGCACCAC 
GACAACTGGA TCCACGGCCC CGGGTCCTGC AAGCTCTTTG GGTTCATCTT CTACACCAAT 
ATCTACATCA GCATCGCCTT CCTGTGCTGC ATCTCGGTGG ACCGCTACCT GGCTGTGGCC 
CACCCACTCC GCTTCGCCCG CCTGCGCCGC GTCAAGACCG CCGTGGCCGT GAGCTCCGTG 
GTCTGGGCCA CGGAGCTGGG CGCCAACTCG GCGCCCCTGT TCCATGACGA GCTCTTCCGA 
GACCGCTACA ACCACACCTT CTGCTTTGAG AAGTTCCCCA TGGAAGGCTG GGTGGCCTGG 
ATGAACCTCT ATCGGGTGTT CGTGGGCTTC CTCTTCCCGT GGGCGCTCAT GCTGCTGTCG 
TACCGGGGCA TCCTGCGGGC CGTGCGGGGC AGCGTGTCCA CCGAGCGCCA GGAGAAGGCC 
AAGATCAAGC GGCTGGCCCT CAGCCTCATC GCCATCGTGC TGGTCTGCTT TGCGCCCTAT 
CACGTGCTCT TGCTGTCCCG CAGCGCCATC TACCTGGGCC GCCCCTGGGA CTGCGGCTTC 
GAGGAGCGCG TCTTTTCTGC ATACCACAGC TCACTGGCTT TCACCAGCCT CAACTGTGTG 
GCGGACCCCA TCCTCTACTG CCTGGTCAAC GAGGGCGCCC GCAGCGATGT GGCCAAGGCC 
CTGCACAACC TGCTCCGCTT TCTGGCCAGC GACAAGCCCC AGGAGATGGC CAATGCCTCG 
CTCACCCTGG AGACCCCACT CACCTCCAAG AGGAACAGCA CAGCCAAAGC CATGACTGGC 
AGCTGGGCGG CCACTCCGCC CTCCCAGGGG GACCAGGTGC AGCTGAAGAT GCTGCCGCCA 
GCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CNS distribution, signalling properties and central effects of G-protein coupled receptor 4.
Neuropharmacology138381-392(2018 08)
Hosford PS,Mosienko V,Kishi K,Jurisic G,Seuwen K,Kinzel B,Ludwig MG,Wells JA,Christie IN,Koolen L,Abdala AP,Liu BH,Gourine AV,Teschemacher AG,Kasparov S

GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOP.
The Biochemical journal474(24)4065-4074(2017 12)
Dong B,Zhang X,Fan Y,Cao S,Zhang X

Manganese and cobalt activate zebrafish ovarian cancer G-protein-coupled receptor 1 but not GPR4.
Journal of receptor and signal transduction research37(4)401-408(2017 Aug)
Negishi J,Omori Y,Shindo M,Takanashi H,Musha S,Nagayama S,Hirayama J,Nishina H,Nakakura T,Mogi C,Sato K,Okajima F,Mochimaru Y,Tomura H

Acidosis Activates Endoplasmic Reticulum Stress Pathways through GPR4 in Human Vascular Endothelial Cells.
International journal of molecular sciences18(2)(2017 Jan)
Dong L,Krewson EA,Yang LV

Human GPR4 and the Notch signaling pathway in endothelial cell tube formation.
Molecular medicine reports14(2)1235-40(2016 Aug)
Ren J,Zhang Y,Cai H,Ma H,Zhao D,Zhang X,Li Z,Wang S,Wang J,Liu R,Li Y,Qian J,Wei H,Niu L,Liu Y,Xiao L,Ding M,Jiang S

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GPR4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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