GOT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GOT1
Entrez Gene ID	2805
Full Name	glutamic-oxaloacetic transaminase 1
Synonyms	AST1,ASTQTL1,GIG18,cAspAT,cCAT
General protein information	Preferred Names glutamic-oxaloacetic transaminase 1 Names aspartate aminotransferase, cytoplasmic aspartate aminotransferase 1 aspartate transaminase 1 cysteine aminotransferase, cytoplasmic cysteine transaminase, cytoplasmic glutamate oxaloacetate transaminase 1 glutamic-oxaloacetic transaminase 1, soluble growth-inhibiting protein 18 testis secretory sperm-binding protein Li 196a transaminase A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q24.2
Summary	Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008].
Disorder MIM:	138180
Disorder Html:	Aspartate aminotransferase, serum level of, QTL1, 614419 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002079.3	NP_002070.1	aspartate aminotransferase, cytoplasmic
NM_002079.2	NP_002070.1	aspartate aminotransferase, cytoplasmic

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Got1	NP_036703.2
Danio rerio (zebrafish)	got1	NP_998222.1
Caenorhabditis elegans (roundworm)	got-1.2	NP_510709.1
Arabidopsis thaliana (thale cress)	ASP3	NP_196713.1
Neurospora crassa	NCU07941	XP_962457.1
Homo sapiens (human)	GOT1	NP_002070.1
Canis lupus familiaris (dog)	GOT1	XP_543963.1
Schizosaccharomyces pombe (fission yeast)	SPAC10F6.13c	NP_593264.1
Kluyveromyces lactis	KLLA0F17754g	XP_455876.1
Magnaporthe oryzae (rice blast fungus)	MGG_04156	XP_003719674.1
Pan troglodytes (chimpanzee)	GOT1	NP_001092011.1
Oryza sativa (rice)	Os01g0760600	NP_001044317.1
Bos taurus (cattle)	GOT1	NP_803468.1
Mus musculus (house mouse)	Got1	NP_034454.2
Gallus gallus (chicken)	GOT1	NP_990652.1
Drosophila melanogaster (fruit fly)	Got1	NP_725534.1
Saccharomyces cerevisiae (baker's yeast)	AAT2	NP_013127.2
Xenopus tropicalis (tropical clawed frog)	got1	NP_998829.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004142	XP_313023.4
Eremothecium gossypii	AGOS_AFR211C	NP_985758.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-9-5p inhibits pancreatic cancer cell proliferation, invasion and glutamine metabolism by targeting GOT1.
Wang J, Wang B, Ren H, Chen W
Biochemical and biophysical research communications509(1)241-248(2019 01)

miR-9 regulates ferroptosis by targeting glutamic-oxaloacetic transaminase GOT1 in melanoma.
Zhang K, Wu L, Zhang P, Luo M, Du J, Gao T, O'Connell D, Wang G, Wang H, Yang Y
Molecular carcinogenesis57(11)1566-1576(2018 11)

Inhibition of glutamate oxaloacetate transaminase 1 in cancer cell lines results in altered metabolism with increased dependency of glucose.
Zhou X, Curbo S, Li F, Krishnan S, Karlsson A
BMC cancer18(1)559(2018 May)

Neither ischemic parenchymal volume nor severe grade complication correlate transient high transaminase elevation after liver resection.
Higaki T, Yamazaki S, Mitsuka Y, Aoki M, Yoshida N, Midorikawa Y, Nakayama H, Takayama T
Bioscience trends12(1)68-72(2018)

Dimerization misalignment in human glutamate-oxaloacetate transaminase variants is the primary factor for PLP release.
Lee J, Gokey T, Ting D, He ZH, Guliaev AB
PloS one13(9)e0203889(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22587	NM_002079.3 Latest version!	Homo sapiens glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu22587	NM_002079.2	Homo sapiens glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GOT1 ( NM_002079.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002070.1 Homo sapiens glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.

Price & Availability↑

CloneID	OHu22587
Clone ID Related Accession (Same CDS sequence)	NM_002079.3 , NM_002079.2
Accession Version	NM_002079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570896000000
Organism	Homo sapiens(human)
Product	aspartate aminotransferase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA581933.1, AL391684.6 and EB387028.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002079.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005609.1, SRR3476690.265247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370508.7/ ENSP00000359539.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACCTC CGTCAGTCTT TGCCGAGGTT CCGCAGGCCC AGCCTGTCCT GGTCTTCAAG 
CTCACTGCCG ACTTCAGGGA GGATCCGGAC CCCCGCAAGG TCAACCTGGG AGTGGGAGCA 
TATCGCACGG ATGACTGCCA TCCCTGGGTT TTGCCAGTAG TGAAGAAAGT GGAGCAGAAG 
ATTGCTAATG ACAATAGCCT AAATCACGAG TATCTGCCAA TCCTGGGCCT GGCTGAGTTC 
CGGAGCTGTG CTTCTCGTCT TGCCCTTGGG GATGACAGCC CAGCACTCAA GGAGAAGCGG 
GTAGGAGGTG TGCAATCTTT GGGGGGAACA GGTGCACTTC GAATTGGAGC TGATTTCTTA 
GCGCGTTGGT ACAATGGAAC AAACAACAAG AACACACCTG TCTATGTGTC CTCACCAACC 
TGGGAGAATC ACAATGCTGT GTTTTCCGCT GCTGGTTTTA AAGACATTCG GTCCTATCGC 
TACTGGGATG CAGAGAAGAG AGGATTGGAC CTCCAGGGCT TCCTGAATGA TCTGGAGAAT 
GCTCCTGAGT TCTCCATTGT TGTCCTCCAC GCCTGTGCAC ACAACCCAAC TGGGATTGAC 
CCAACTCCGG AGCAGTGGAA GCAGATTGCT TCTGTCATGA AGCACCGGTT TCTGTTCCCC 
TTCTTTGACT CAGCCTATCA GGGCTTCGCA TCTGGAAACC TGGAGAGAGA TGCCTGGGCC 
ATTCGCTATT TTGTGTCTGA AGGCTTCGAG TTCTTCTGTG CCCAGTCCTT CTCCAAGAAC 
TTCGGGCTCT ACAATGAGAG AGTCGGGAAT CTGACTGTGG TTGGAAAAGA ACCTGAGAGC 
ATCCTGCAAG TCCTTTCCCA GATGGAGAAG ATCGTGCGGA TTACTTGGTC CAATCCCCCC 
GCCCAGGGAG CACGAATTGT GGCCAGCACC CTCTCTAACC CTGAGCTCTT TGAGGAATGG 
ACAGGTAATG TGAAGACAAT GGCTGACCGG ATTCTGACCA TGAGATCTGA ACTCAGGGCA 
CGACTAGAAG CCCTCAAAAC CCCTGGGACC TGGAACCACA TCACTGATCA AATTGGCATG 
TTCAGCTTCA CTGGGTTGAA CCCCAAGCAG GTTGAGTATC TGGTCAATGA AAAGCACATC 
TACCTGCTGC CAAGTGGTCG AATCAACGTG AGTGGCTTAA CCACCAAAAA TCTAGATTAC 
GTGGCCACCT CCATCCATGA AGCAGTCACC AAAATCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002070.1
CDS	60..1301
Translation	MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ

Target ORF information:

RefSeq Version	NM_002079.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002079.3

ORF Insert Sequence:

ATGGCACCTC CGTCAGTCTT TGCCGAGGTT CCGCAGGCCC AGCCTGTCCT GGTCTTCAAG 
CTCACTGCCG ACTTCAGGGA GGATCCGGAC CCCCGCAAGG TCAACCTGGG AGTGGGAGCA 
TATCGCACGG ATGACTGCCA TCCCTGGGTT TTGCCAGTAG TGAAGAAAGT GGAGCAGAAG 
ATTGCTAATG ACAATAGCCT AAATCACGAG TATCTGCCAA TCCTGGGCCT GGCTGAGTTC 
CGGAGCTGTG CTTCTCGTCT TGCCCTTGGG GATGACAGCC CAGCACTCAA GGAGAAGCGG 
GTAGGAGGTG TGCAATCTTT GGGGGGAACA GGTGCACTTC GAATTGGAGC TGATTTCTTA 
GCGCGTTGGT ACAATGGAAC AAACAACAAG AACACACCTG TCTATGTGTC CTCACCAACC 
TGGGAGAATC ACAATGCTGT GTTTTCCGCT GCTGGTTTTA AAGACATTCG GTCCTATCGC 
TACTGGGATG CAGAGAAGAG AGGATTGGAC CTCCAGGGCT TCCTGAATGA TCTGGAGAAT 
GCTCCTGAGT TCTCCATTGT TGTCCTCCAC GCCTGTGCAC ACAACCCAAC TGGGATTGAC 
CCAACTCCGG AGCAGTGGAA GCAGATTGCT TCTGTCATGA AGCACCGGTT TCTGTTCCCC 
TTCTTTGACT CAGCCTATCA GGGCTTCGCA TCTGGAAACC TGGAGAGAGA TGCCTGGGCC 
ATTCGCTATT TTGTGTCTGA AGGCTTCGAG TTCTTCTGTG CCCAGTCCTT CTCCAAGAAC 
TTCGGGCTCT ACAATGAGAG AGTCGGGAAT CTGACTGTGG TTGGAAAAGA ACCTGAGAGC 
ATCCTGCAAG TCCTTTCCCA GATGGAGAAG ATCGTGCGGA TTACTTGGTC CAATCCCCCC 
GCCCAGGGAG CACGAATTGT GGCCAGCACC CTCTCTAACC CTGAGCTCTT TGAGGAATGG 
ACAGGTAATG TGAAGACAAT GGCTGACCGG ATTCTGACCA TGAGATCTGA ACTCAGGGCA 
CGACTAGAAG CCCTCAAAAC CCCTGGGACC TGGAACCACA TCACTGATCA AATTGGCATG 
TTCAGCTTCA CTGGGTTGAA CCCCAAGCAG GTTGAGTATC TGGTCAATGA AAAGCACATC 
TACCTGCTGC CAAGTGGTCG AATCAACGTG AGTGGCTTAA CCACCAAAAA TCTAGATTAC 
GTGGCCACCT CCATCCATGA AGCAGTCACC AAAATCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GOT1 ( NM_002079.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002070.1 Homo sapiens glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.

Price & Availability↑

CloneID	OHu22587
Clone ID Related Accession (Same CDS sequence)	NM_002079.3 , NM_002079.2
Accession Version	NM_002079.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509292800000
Organism	Homo sapiens(human)
Product	aspartate aminotransferase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA581933.1, AL391684.6 and EB387028.1. This sequence is a reference standard in the RefSeqGene project. On Aug 27, 2008 this sequence version replaced NM_002079.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005609.1, SRR1803611.67470.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCTC CGTCAGTCTT TGCCGAGGTT CCGCAGGCCC AGCCTGTCCT GGTCTTCAAG 
CTCACTGCCG ACTTCAGGGA GGATCCGGAC CCCCGCAAGG TCAACCTGGG AGTGGGAGCA 
TATCGCACGG ATGACTGCCA TCCCTGGGTT TTGCCAGTAG TGAAGAAAGT GGAGCAGAAG 
ATTGCTAATG ACAATAGCCT AAATCACGAG TATCTGCCAA TCCTGGGCCT GGCTGAGTTC 
CGGAGCTGTG CTTCTCGTCT TGCCCTTGGG GATGACAGCC CAGCACTCAA GGAGAAGCGG 
GTAGGAGGTG TGCAATCTTT GGGGGGAACA GGTGCACTTC GAATTGGAGC TGATTTCTTA 
GCGCGTTGGT ACAATGGAAC AAACAACAAG AACACACCTG TCTATGTGTC CTCACCAACC 
TGGGAGAATC ACAATGCTGT GTTTTCCGCT GCTGGTTTTA AAGACATTCG GTCCTATCGC 
TACTGGGATG CAGAGAAGAG AGGATTGGAC CTCCAGGGCT TCCTGAATGA TCTGGAGAAT 
GCTCCTGAGT TCTCCATTGT TGTCCTCCAC GCCTGTGCAC ACAACCCAAC TGGGATTGAC 
CCAACTCCGG AGCAGTGGAA GCAGATTGCT TCTGTCATGA AGCACCGGTT TCTGTTCCCC 
TTCTTTGACT CAGCCTATCA GGGCTTCGCA TCTGGAAACC TGGAGAGAGA TGCCTGGGCC 
ATTCGCTATT TTGTGTCTGA AGGCTTCGAG TTCTTCTGTG CCCAGTCCTT CTCCAAGAAC 
TTCGGGCTCT ACAATGAGAG AGTCGGGAAT CTGACTGTGG TTGGAAAAGA ACCTGAGAGC 
ATCCTGCAAG TCCTTTCCCA GATGGAGAAG ATCGTGCGGA TTACTTGGTC CAATCCCCCC 
GCCCAGGGAG CACGAATTGT GGCCAGCACC CTCTCTAACC CTGAGCTCTT TGAGGAATGG 
ACAGGTAATG TGAAGACAAT GGCTGACCGG ATTCTGACCA TGAGATCTGA ACTCAGGGCA 
CGACTAGAAG CCCTCAAAAC CCCTGGGACC TGGAACCACA TCACTGATCA AATTGGCATG 
TTCAGCTTCA CTGGGTTGAA CCCCAAGCAG GTTGAGTATC TGGTCAATGA AAAGCACATC 
TACCTGCTGC CAAGTGGTCG AATCAACGTG AGTGGCTTAA CCACCAAAAA TCTAGATTAC 
GTGGCCACCT CCATCCATGA AGCAGTCACC AAAATCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002070.1
CDS	209..1450
Translation	MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ

Target ORF information:

RefSeq Version	NM_002079.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamic-oxaloacetic transaminase 1 (GOT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002079.2

ORF Insert Sequence:

ATGGCACCTC CGTCAGTCTT TGCCGAGGTT CCGCAGGCCC AGCCTGTCCT GGTCTTCAAG 
CTCACTGCCG ACTTCAGGGA GGATCCGGAC CCCCGCAAGG TCAACCTGGG AGTGGGAGCA 
TATCGCACGG ATGACTGCCA TCCCTGGGTT TTGCCAGTAG TGAAGAAAGT GGAGCAGAAG 
ATTGCTAATG ACAATAGCCT AAATCACGAG TATCTGCCAA TCCTGGGCCT GGCTGAGTTC 
CGGAGCTGTG CTTCTCGTCT TGCCCTTGGG GATGACAGCC CAGCACTCAA GGAGAAGCGG 
GTAGGAGGTG TGCAATCTTT GGGGGGAACA GGTGCACTTC GAATTGGAGC TGATTTCTTA 
GCGCGTTGGT ACAATGGAAC AAACAACAAG AACACACCTG TCTATGTGTC CTCACCAACC 
TGGGAGAATC ACAATGCTGT GTTTTCCGCT GCTGGTTTTA AAGACATTCG GTCCTATCGC 
TACTGGGATG CAGAGAAGAG AGGATTGGAC CTCCAGGGCT TCCTGAATGA TCTGGAGAAT 
GCTCCTGAGT TCTCCATTGT TGTCCTCCAC GCCTGTGCAC ACAACCCAAC TGGGATTGAC 
CCAACTCCGG AGCAGTGGAA GCAGATTGCT TCTGTCATGA AGCACCGGTT TCTGTTCCCC 
TTCTTTGACT CAGCCTATCA GGGCTTCGCA TCTGGAAACC TGGAGAGAGA TGCCTGGGCC 
ATTCGCTATT TTGTGTCTGA AGGCTTCGAG TTCTTCTGTG CCCAGTCCTT CTCCAAGAAC 
TTCGGGCTCT ACAATGAGAG AGTCGGGAAT CTGACTGTGG TTGGAAAAGA ACCTGAGAGC 
ATCCTGCAAG TCCTTTCCCA GATGGAGAAG ATCGTGCGGA TTACTTGGTC CAATCCCCCC 
GCCCAGGGAG CACGAATTGT GGCCAGCACC CTCTCTAACC CTGAGCTCTT TGAGGAATGG 
ACAGGTAATG TGAAGACAAT GGCTGACCGG ATTCTGACCA TGAGATCTGA ACTCAGGGCA 
CGACTAGAAG CCCTCAAAAC CCCTGGGACC TGGAACCACA TCACTGATCA AATTGGCATG 
TTCAGCTTCA CTGGGTTGAA CCCCAAGCAG GTTGAGTATC TGGTCAATGA AAAGCACATC 
TACCTGCTGC CAAGTGGTCG AATCAACGTG AGTGGCTTAA CCACCAAAAA TCTAGATTAC 
GTGGCCACCT CCATCCATGA AGCAGTCACC AAAATCCAGT GA