USP12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP12
Entrez Gene ID	219333
Full Name	ubiquitin specific peptidase 12
Synonyms	UBH1,USP12L1
General protein information	Preferred Names ubiquitin specific peptidase 12 Names ubiquitin carboxyl-terminal hydrolase 12 deubiquitinating enzyme 12 ubiquitin specific protease 12 like 1 ubiquitin thioesterase 12 ubiquitin thiolesterase 12 ubiquitin-hydrolyzing enzyme 1 ubiquitin-specific-processing protease 12
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.13
Disorder MIM:	603091

mRNA and Protein(s)

mRNA	Protein	Name
NM_182488.4	NP_872294.2	ubiquitin carboxyl-terminal hydrolase 12
XM_017020416.1	XP_016875905.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X2
NM_182488.3	NP_872294.2	ubiquitin carboxyl-terminal hydrolase 12
XM_011534956.2	XP_011533258.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X2
XM_005266282.2	XP_005266339.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	ubp9	NP_596207.1
Homo sapiens (human)	USP12	NP_872294.2
Bos taurus (cattle)	USP12	NP_001091528.1
Rattus norvegicus (Norway rat)	Usp12	NP_001160048.1
Oryza sativa (rice)	Os03g0190800	NP_001049228.1
Pan troglodytes (chimpanzee)	USP12	XP_001135418.2
Canis lupus familiaris (dog)	USP12	XP_543159.2
Mus musculus (house mouse)	Usp12	NP_035799.1
Gallus gallus (chicken)	USP12	XP_004938833.1
Danio rerio (zebrafish)	usp12a	NP_001077025.1
Xenopus tropicalis (tropical clawed frog)	LOC100494517	XP_002941584.2
Neurospora crassa	NCU08378	XP_965281.2
Magnaporthe oryzae (rice blast fungus)	MGG_09907	XP_003710004.1
Macaca mulatta (Rhesus monkey)	LOC721611	XP_001117796.2

Gene Ontology
Bibliography

Related articles in PubMed

WDR20 regulates shuttling of the USP12 deubiquitinase complex between the plasma membrane, cytoplasm and nucleus.
Olazabal-Herrero A, Sendino M, Arganda-Carreras I, Rodríguez JA
European journal of cell biology98(1)12-26(2019 Jan)

Deubiquitinase Usp12 functions noncatalytically to induce autophagy and confer neuroprotection in models of Huntington's disease.
Aron R, Pellegrini P, Green EW, Maddison DC, Opoku-Nsiah K, Wong JS, Daub AC, Giorgini F, Finkbeiner S
Nature communications9(1)3191(2018 09)

Molecular mechanism of the TP53-MDM2-AR-AKT signalling network regulation by USP12.
McClurg UL, Chit NCTH, Azizyan M, Edwards J, Nabbi A, Riabowol KT, Nakjang S, McCracken SR, Robson CN
Oncogene37(34)4679-4691(2018 08)

A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme.
Dharadhar S, Clerici M, van Dijk WJ, Fish A, Sixma TK
Journal of structural biology196(3)437-447(2016 12)

Allosteric Activation of Ubiquitin-Specific Proteases by β-Propeller Proteins UAF1 and WDR20.
Li H, Lim KS, Kim H, Hinds TR, Jo U, Mao H, Weller CE, Sun J, Chatterjee C, D'Andrea AD, Zheng N
Molecular cell63(2)249-260(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following USP12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu47092	XM_005266282.2 Latest version!	Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu77883	XM_011534956.2 Latest version!	Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu77883	XM_017020416.1 Latest version!	Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu11192	NM_182488.4 Latest version!	Homo sapiens ubiquitin specific peptidase 12 (USP12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu11192	NM_182488.3	Homo sapiens ubiquitin specific peptidase 12 (USP12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP12 ( XM_005266282.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005266339.1 Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu47092
Clone ID Related Accession (Same CDS sequence)	XM_005266282.2
Accession Version	XM_005266282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005266282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCT 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAATGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AATTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTTGCGTAT AAGAGTCAAC CTAGGAAAAA GGAGAGCCTT 
CTTACATGCT TAGCAGATCT CTTCCATAGC ATAGCCACTC AGAAGAAAAA GGTTGGAGTA 
ATACCCCCTA AGAAGTTCAT CACAAGATTA CGGAAAGAAA ATGAGCTTTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005266339.1
CDS	172..1110
Translation	MEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKESLLTCLADLFHSIATQKKKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADILQEERKQEKQNGRLPNGNIDNENNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGRK

Target ORF information:

RefSeq Version	XM_005266282.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005266282.2

ORF Insert Sequence:

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCT 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAATGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AATTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTTGCGTAT AAGAGTCAAC CTAGGAAAAA GGAGAGCCTT 
CTTACATGCT TAGCAGATCT CTTCCATAGC ATAGCCACTC AGAAGAAAAA GGTTGGAGTA 
ATACCCCCTA AGAAGTTCAT CACAAGATTA CGGAAAGAAA ATGAGCTTTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP12 ( XM_017020416.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875905.1 Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu77883
Clone ID Related Accession (Same CDS sequence)	XM_017020416.1 , XM_011534956.2
Accession Version	XM_017020416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875905.1
CDS	289..1041
Translation	MQQDAHEFLNYLLNTIADILQEERKQEKQNGRLPNGNIDNENNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	XM_017020416.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020416.1

ORF Insert Sequence:

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP12 ( XM_011534956.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533258.1 Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu77883
Clone ID Related Accession (Same CDS sequence)	XM_017020416.1 , XM_011534956.2
Accession Version	XM_011534956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533258.1
CDS	369..1121
Translation	MQQDAHEFLNYLLNTIADILQEERKQEKQNGRLPNGNIDNENNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	XM_011534956.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 12 (USP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534956.2

ORF Insert Sequence:

ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP12 ( NM_182488.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_872294.2 Homo sapiens ubiquitin specific peptidase 12 (USP12), mRNA.

Price & Availability↑

CloneID	OHu11192
Clone ID Related Accession (Same CDS sequence)	NM_182488.4 , NM_182488.3
Accession Version	NM_182488.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556640000000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC026072.1, AF022789.1 and AL355473.20. On Nov 23, 2018 this sequence version replaced NM_182488.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF022789.1, SRR1803617.159262.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000282344.11/ ENSP00000282344.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCT 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAATGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AATTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTTGCGTAT AAGAGTCAAC CTAGGAAAAA GGAGAGCCTT 
CTTACATGCT TAGCAGATCT CTTCCATAGC ATAGCCACTC AGAAGAAAAA GGTTGGAGTA 
ATACCCCCTA AGAAGTTCAT CACAAGATTA CGGAAAGAAA ATGAGCTTTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872294.2
CDS	173..1285
Translation	MEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKESLLTCLADLFHSIATQKKKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADILQEERKQEKQNGRLPNGNIDNENNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	NM_182488.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 12 (USP12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182488.4

ORF Insert Sequence:

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCT 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAATGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AATTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTTGCGTAT AAGAGTCAAC CTAGGAAAAA GGAGAGCCTT 
CTTACATGCT TAGCAGATCT CTTCCATAGC ATAGCCACTC AGAAGAAAAA GGTTGGAGTA 
ATACCCCCTA AGAAGTTCAT CACAAGATTA CGGAAAGAAA ATGAGCTTTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP12 ( NM_182488.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_872294.2 Homo sapiens ubiquitin specific peptidase 12 (USP12), mRNA.

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CloneID	OHu11192
Clone ID Related Accession (Same CDS sequence)	NM_182488.4 , NM_182488.3
Accession Version	NM_182488.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514390400000
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL158062.22, BC026072.1, AF022789.1 and AL355473.20. On Jul 28, 2010 this sequence version replaced NM_182488.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF022789.1, SRR1803617.159262.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCT 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAATGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AATTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTTGCGTAT AAGAGTCAAC CTAGGAAAAA GGAGAGCCTT 
CTTACATGCT TAGCAGATCT CTTCCATAGC ATAGCCACTC AGAAGAAAAA GGTTGGAGTA 
ATACCCCCTA AGAAGTTCAT CACAAGATTA CGGAAAGAAA ATGAGCTTTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_872294.2
CDS	258..1370
Translation	MEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKESLLTCLADLFHSIATQKKKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADILQEERKQEKQNGRLPNGNIDNENNNSTPDPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

Target ORF information:

RefSeq Version	NM_182488.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 12 (USP12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182488.3

ORF Insert Sequence:

ATGGAAATCC TAATGACAGT CTCCAAATTC GCCTCCATCT GTACCATGGG CGCCAATGCT 
TCGGCATTAG AGAAAGAGAT TGGTCCAGAA CAGTTTCCGG TCAATGAGCA CTATTTTGGA 
TTAGTCAATT TTGGGAATAC CTGCTACTGC AATTCAGTTC TTCAAGCACT TTATTTTTGT 
CGTCCATTTC GGGAAAAAGT TCTTGCGTAT AAGAGTCAAC CTAGGAAAAA GGAGAGCCTT 
CTTACATGCT TAGCAGATCT CTTCCATAGC ATAGCCACTC AGAAGAAAAA GGTTGGAGTA 
ATACCCCCTA AGAAGTTCAT CACAAGATTA CGGAAAGAAA ATGAGCTTTT TGACAACTAC 
ATGCAACAAG ATGCCCATGA ATTCTTAAAT TACCTACTAA ATACAATTGC TGATATTTTA 
CAAGAAGAGA GAAAGCAGGA AAAACAAAAT GGTCGTTTAC CTAATGGTAA TATTGATAAT 
GAAAATAATA ACAGCACACC AGACCCAACG TGGGTTCATG AGATTTTTCA GGGAACATTA 
ACTAATGAAA CCAGATGTCT TACTTGTGAA ACTATAAGCA GCAAAGATGA AGATTTTTTA 
GACCTTTCTG TTGACGTGGA ACAAAATACA TCAATTACTC ACTGCTTAAG GGGTTTCAGC 
AACACAGAAA CTCTGTGCAG TGAATACAAG TATTACTGTG AAGAGTGTCG CAGCAAACAG 
GAAGCACACA AACGGATGAA AGTTAAAAAA CTGCCCATGA TTCTAGCTCT ACACCTGAAG 
AGATTTAAAT ATATGGATCA ACTTCATCGA TATACAAAAC TCTCTTACCG GGTAGTTTTT 
CCTTTAGAAC TTCGTCTGTT TAACACTTCA GGTGATGCCA CCAATCCAGA CAGAATGTAC 
GACCTTGTTG CTGTTGTGGT TCACTGTGGA AGTGGTCCCA ATCGAGGCCA TTATATTGCA 
ATAGTTAAGA GTCATGATTT TTGGTTGTTG TTTGATGACG ACATTGTAGA AAAAATAGAT 
GCACAAGCTA TTGAAGAATT CTACGGGTTG ACATCAGATA TCTCAAAGAA CTCTGAGTCT 
GGTTACATCC TTTTCTATCA GTCTCGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WDR20 regulates shuttling of the USP12 deubiquitinase complex between the plasma membrane, cytoplasm and nucleus.
European journal of cell biology98(1)12-26(2019 Jan)
Olazabal-Herrero A,Sendino M,Arganda-Carreras I,Rodríguez JA

Deubiquitinase Usp12 functions noncatalytically to induce autophagy and confer neuroprotection in models of Huntington's disease.
Nature communications9(1)3191(2018 09)
Aron R,Pellegrini P,Green EW,Maddison DC,Opoku-Nsiah K,Wong JS,Daub AC,Giorgini F,Finkbeiner S

Molecular mechanism of the TP53-MDM2-AR-AKT signalling network regulation by USP12.
Oncogene37(34)4679-4691(2018 08)
McClurg UL,Chit NCTH,Azizyan M,Edwards J,Nabbi A,Riabowol KT,Nakjang S,McCracken SR,Robson CN

A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme.
Journal of structural biology196(3)437-447(2016 12)
Dharadhar S,Clerici M,van Dijk WJ,Fish A,Sixma TK

Allosteric Activation of Ubiquitin-Specific Proteases by β-Propeller Proteins UAF1 and WDR20.
Molecular cell63(2)249-260(2016 07)
Li H,Lim KS,Kim H,Hinds TR,Jo U,Mao H,Weller CE,Sun J,Chatterjee C,D'Andrea AD,Zheng N

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USP12 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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