TYMP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TYMP
Entrez Gene ID	1890
Full Name	thymidine phosphorylase
Synonyms	ECGF,ECGF1,MEDPS1,MNGIE,MTDPS1,PDECGF,TP,hPD-ECGF
General protein information	Preferred Names thymidine phosphorylase Names thymidine phosphorylase gliostatin tdRPase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.33
Summary	This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012].
Disorder MIM:	131222
Disorder Html:	Mitochondrial DNA depletion syndrome 1 (MNGIE type), 603041 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113756.2	NP_001107228.1	thymidine phosphorylase isoform 1 precursor
NM_001953.4	NP_001944.1	thymidine phosphorylase isoform 1 precursor
NM_001257988.1	NP_001244917.1	thymidine phosphorylase isoform 1 precursor
NM_001257989.1	NP_001244918.1	thymidine phosphorylase isoform 2
NM_001113755.2	NP_001107227.1	thymidine phosphorylase isoform 1 precursor
NM_001113756.3	NP_001107228.1	thymidine phosphorylase isoform 1 precursor
NM_001113755.3	NP_001107227.1	thymidine phosphorylase isoform 1 precursor
NM_001953.5	NP_001944.1	thymidine phosphorylase isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYMP	NP_001107228.1
Pan troglodytes (chimpanzee)	TYMP	XP_515224.4
Rattus norvegicus (Norway rat)	Tymp	NP_001012122.1
Mus musculus (house mouse)	Tymp	NP_612175.1
Xenopus tropicalis (tropical clawed frog)	tymp	NP_001120427.1
Macaca mulatta (Rhesus monkey)	TYMP	XP_001112945.1

Gene Ontology
Bibliography

Related articles in PubMed

5-Aza-2-deoxycytidine Enhances the Sensitivity of 5-Fluorouracil by Demethylation of the Thymidine Phosphorylase Promoter.
Nishizawa Y, Ikeda R, Yamamoto M, Kawahara K, Shinsato Y, Minami K, Nitta M, Terazono H, Akiyama SI, Furukawa T, Takeda Y
Anticancer research39(8)4129-4136(2019 Aug)

Thymidine phosphorylase promotes metastasis and serves as a marker of poor prognosis in hepatocellular carcinoma.
Zhang Q, Zhang Y, Hu X, Qin Y, Zhong W, Meng J, Xiao T, Zhang C, Li M, Chen S, Liu H, Liu Y, Sun T, Yang C
Laboratory investigation; a journal of technical methods and pathology97(8)903-912(2017 08)

Poor Prognosis Associated with High Levels of Thymidine Phosphorylase and Thrombocytosis in Patients with Renal Cell Carcinoma.
Huang X, Wang L, Chen Y, Zheng X, Wang X
Urologia internationalis98(2)162-168(2017)

Pinpointing a Factor in Myeloma Bone Disease.

Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma.
Liu H, Liu Z, Du J, He J, Lin P, Amini B, Starbuck MW, Novane N, Shah JJ, Davis RE, Hou J, Gagel RF, Yang J
Science translational medicine8(353)353ra113(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TYMP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TYMP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22602	NM_001113756.3 Latest version!	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu22602	NM_001257988.1 Latest version!	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu22602	NM_001953.5 Latest version!	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu22602	NM_001113755.3 Latest version!	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu23077	NM_001257989.1 Latest version!	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu22602	NM_001113756.2	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu22602	NM_001113755.2	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu22602	NM_001953.4	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TYMP ( NM_001113756.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107228.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.

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CloneID	OHu22602
Clone ID Related Accession (Same CDS sequence)	NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version	NM_001113756.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, M63193.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced NM_001113756.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.300347.1, ERR279847.3916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107228.1
CDS	377..1825
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001113756.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113756.2

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYMP ( NM_001953.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001944.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22602
Clone ID Related Accession (Same CDS sequence)	NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version	NM_001953.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC018160.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced NM_001953.3. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018160.1, SRR1163655.527340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001944.1
CDS	193..1641
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001953.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001953.4

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYMP ( NM_001257988.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244917.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22602
Clone ID Related Accession (Same CDS sequence)	NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version	NM_001257988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC052211.2 and AK225269.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052211.2, SRR1163658.312218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2153980 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END##

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244917.1
CDS	228..1676
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001257988.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257988.1

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYMP ( NM_001113755.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107227.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22602
Clone ID Related Accession (Same CDS sequence)	NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version	NM_001113755.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, AK314716.1, BC052211.2 and BM744178.1. On Apr 13, 2012 this sequence version replaced NM_001113755.1. Transcript Variant: This variant (1) and variants 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.175764.1, AK314716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107227.1
CDS	205..1653
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001113755.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113755.2

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYMP ( NM_001113756.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107228.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22602
Clone ID Related Accession (Same CDS sequence)	NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version	NM_001113756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, M63193.1, BC052211.2 and BM744178.1. On Jun 2, 2019 this sequence version replaced NM_001113756.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138517.532115.1, SRR1163655.300347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107228.1
CDS	304..1752
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001113756.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113756.3

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYMP ( NM_001113755.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107227.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22602
Clone ID Related Accession (Same CDS sequence)	NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version	NM_001113755.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314716.1, BC052211.2 and BM744178.1. On Jun 2, 2019 this sequence version replaced NM_001113755.2. Transcript Variant: This variant (1) and variants 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314716.1, SRR1163655.175764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107227.1
CDS	132..1580
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001113755.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113755.3

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYMP ( NM_001953.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001944.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.

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CloneID	OHu22602
Clone ID Related Accession (Same CDS sequence)	NM_001113756.2 , NM_001953.4 , NM_001257988.1 , NM_001113755.2 , NM_001113756.3 , NM_001113755.3 , NM_001953.5
Accession Version	NM_001953.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, BC018160.1, BC052211.2 and BM744178.1. On Jun 2, 2019 this sequence version replaced NM_001953.4. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018160.1, SRR7346977.97705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001944.1
CDS	120..1568
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001953.5
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001953.5

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG 
CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC 
GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG 
CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA 
CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG 
CAGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYMP ( NM_001257989.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244918.1 Homo sapiens thymidine phosphorylase (TYMP), transcript variant 5, mRNA.

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CloneID	OHu23077
Clone ID Related Accession (Same CDS sequence)	NM_001257989.1
Accession Version	NM_001257989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565539200000
Organism	Homo sapiens(human)
Product	thymidine phosphorylase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U62317.2, AK225269.1, BC052211.2 and BM744178.1. Transcript Variant: This variant (5) uses alternate splice sites in the 5' UTR and the 3' coding region, compared to variant 1. The resulting isoform (2) has an additional segment in the C-terminal region, compared to isoform 1. It is not known whether this isoform (2) is proteolytically processed in the same manner as isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGC CCCTCTCCCC GCAGGCACCG TGGAGCTGGT CCGGGCGCTG 
CCGCTGGCGC TGGTGCTGCA CGAGCTCGGG GCCGGGCGCA GCCGCGCTGG GGAGCCGCTC 
CGCCTGGGGG TGGGCGCAGA GCTGCTGGTC GACGTGGGTC AGAGGCTGCG CCGTGGGACC 
CCCTGGCTCC GCGTGCACCG GGACGGCCCC GCGCTCAGCG GCCCGCAGAG CCGCGCCCTG 
CAGGAGGCGC TCGTACTCTC CGACCGCGCG CCATTCGCCG CCCCCTCGCC CTTCGCAGAG 
CTCGTTCTGC CGCCGCAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244918.1
CDS	193..1656
Translation	MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADAPLPAGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

Target ORF information:

RefSeq Version	NM_001257989.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thymidine phosphorylase (TYMP), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257989.1

ORF Insert Sequence:

ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG 
GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC 
CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT 
GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT 
CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG 
CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT 
GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG 
CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT 
ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG 
GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA 
GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT 
AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC 
GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC 
AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC 
GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA 
GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG 
GGGACTCAGG CCCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT 
GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG 
TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG 
CTGCTGGCGC CCGCAGATGC CCCTCTCCCC GCAGGCACCG TGGAGCTGGT CCGGGCGCTG 
CCGCTGGCGC TGGTGCTGCA CGAGCTCGGG GCCGGGCGCA GCCGCGCTGG GGAGCCGCTC 
CGCCTGGGGG TGGGCGCAGA GCTGCTGGTC GACGTGGGTC AGAGGCTGCG CCGTGGGACC 
CCCTGGCTCC GCGTGCACCG GGACGGCCCC GCGCTCAGCG GCCCGCAGAG CCGCGCCCTG 
CAGGAGGCGC TCGTACTCTC CGACCGCGCG CCATTCGCCG CCCCCTCGCC CTTCGCAGAG 
CTCGTTCTGC CGCCGCAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

5-Aza-2-deoxycytidine Enhances the Sensitivity of 5-Fluorouracil by Demethylation of the Thymidine Phosphorylase Promoter.
Anticancer research39(8)4129-4136(2019 Aug)
Nishizawa Y,Ikeda R,Yamamoto M,Kawahara K,Shinsato Y,Minami K,Nitta M,Terazono H,Akiyama SI,Furukawa T,Takeda Y

Thymidine phosphorylase promotes metastasis and serves as a marker of poor prognosis in hepatocellular carcinoma.
Laboratory investigation; a journal of technical methods and pathology97(8)903-912(2017 08)
Zhang Q,Zhang Y,Hu X,Qin Y,Zhong W,Meng J,Xiao T,Zhang C,Li M,Chen S,Liu H,Liu Y,Sun T,Yang C

Poor Prognosis Associated with High Levels of Thymidine Phosphorylase and Thrombocytosis in Patients with Renal Cell Carcinoma.
Urologia internationalis98(2)162-168(2017)
Huang X,Wang L,Chen Y,Zheng X,Wang X

Pinpointing a Factor in Myeloma Bone Disease.
Cancer discovery6(11)1201-1202(2016 11)

Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma.
Science translational medicine8(353)353ra113(2016 08)
Liu H,Liu Z,Du J,He J,Lin P,Amini B,Starbuck MW,Novane N,Shah JJ,Davis RE,Hou J,Gagel RF,Yang J

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TYMP cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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