DKC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DKC1
Entrez Gene ID	1736
Full Name	dyskerin pseudouridine synthase 1
Synonyms	CBF5,DKC,DKCX,NAP57,NOLA4,XAP101
General protein information	Preferred Names dyskerin pseudouridine synthase 1 Names H/ACA ribonucleoprotein complex subunit 4 CBF5 homolog dyskeratosis congenita 1, dyskerin nopp140-associated protein of 57 kDa nucleolar protein NAP57 nucleolar protein family A member 4 snoRNP protein DKC1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq28
Summary	This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].
Disorder MIM:	300126
Disorder Html:	Dyskeratosis congenita, X-linked, 305000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142463.3	NP_001135935.1	H/ACA ribonucleoprotein complex subunit DKC1 isoform 2
NM_001363.5	NP_001354.1	H/ACA ribonucleoprotein complex subunit DKC1 isoform 1
NM_001288747.1	NP_001275676.1	H/ACA ribonucleoprotein complex subunit DKC1 isoform 3
NM_001142463.2	NP_001135935.1	H/ACA ribonucleoprotein complex subunit DKC1 isoform 2
NM_001363.4	NP_001354.1	H/ACA ribonucleoprotein complex subunit 4 isoform 1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	DKC1	XP_001090867.2
Mus musculus (house mouse)	Dkc1	NP_001025478.1
Gallus gallus (chicken)	DKC1	NP_001026286.1
Caenorhabditis elegans (roundworm)	K01G5.5	NP_499370.1
Magnaporthe oryzae (rice blast fungus)	MGG_07518	XP_003711432.1
Oryza sativa (rice)	Os07g0636000	NP_001060391.1
Rattus norvegicus (Norway rat)	Dkc1	NP_596910.1
Drosophila melanogaster (fruit fly)	Nop60B	NP_001163289.1
Kluyveromyces lactis	KLLA0D04796g	XP_453273.1
Eremothecium gossypii	AGOS_AAL007C	NP_982535.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004739	XP_318082.4
Pan troglodytes (chimpanzee)	DKC1	XP_521345.3
Bos taurus (cattle)	DKC1	NP_001098865.1
Saccharomyces cerevisiae (baker's yeast)	CBF5	NP_013276.1
Arabidopsis thaliana (thale cress)	NAP57	NP_191274.1
Neurospora crassa	NCU01290	XP_960827.1
Xenopus tropicalis (tropical clawed frog)	dkc1	XP_004916830.1
Canis lupus familiaris (dog)	DKC1	XP_549382.2
Danio rerio (zebrafish)	dkc1	NP_001028279.2
Oryza sativa (rice)	Os03g0370500	NP_001050199.1
Schizosaccharomyces pombe (fission yeast)	cbf5	NP_594878.1
Homo sapiens (human)	DKC1	NP_001354.1

Gene Ontology
Bibliography

Related articles in PubMed

N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation.
MacNeil DE, Lambert-Lanteigne P, Autexier C
Nucleic acids research47(10)5368-5380(2019 06)

Characterization of an X-chromosome-linked telomere biology disorder in females with DKC1 mutation.
Hirvonen EAM, Peuhkuri S, Norberg A, Degerman S, Hannula-Jouppi K, Välimaa H, Kilpivaara O, Wartiovaara-Kautto U
Leukemia33(1)275-278(2019 01)

DKC1 serves as a potential prognostic biomarker for human clear cell renal cell carcinoma and promotes its proliferation, migration and invasion via the NF‑κB pathway.
Zhang M, Pan Y, Jiang R, Hou P, Shan H, Chen F, Jiang T, Bai J, Zheng J
Oncology reports40(2)968-978(2018 Aug)

Dyskeratosis congenita with a novel genetic variant in the DKC1 gene: a case report.
Ratnasamy V, Navaneethakrishnan S, Sirisena ND, Grüning NM, Brandau O, Thirunavukarasu K, Dagnall CL, McReynolds LJ, Savage SA, Dissanayake VHW
BMC medical genetics19(1)85(2018 05)

Cryo-EM structure of substrate-bound human telomerase holoenzyme.
Nguyen THD, Tam J, Wu RA, Greber BJ, Toso D, Nogales E, Collins K
Nature557(7704)190-195(2018 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DKC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DKC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu107622	NM_001363.5 Latest version!	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu108434	NM_001142463.3 Latest version!	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu23916	NM_001142463.2 Latest version!	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu23474	NM_001288747.1 Latest version!	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu23856	NM_001363.4 Latest version!	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DKC1 ( NM_001363.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354.1 Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107622
Clone ID Related Accession (Same CDS sequence)	NM_001363.5
Accession Version	NM_001363.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit DKC1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC109993.4. On Nov 23, 2018 this sequence version replaced NM_001363.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.57806.1, BC009928.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369550.10/ ENSP00000358563.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACGC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGAGTATGT TGACTACAGT 
GAGTCTGCCA AAAAAGAGGT GGTTGCTGAA GTGGTAAAAG CCCCGCAGGT AGTTGCCGAA 
GCAGCAAAAA CTGCGAAGCG GAAGCGAGAG AGTGAGAGTG AAAGTGACGA GACTCCTCCA 
GCAGCTCCTC AGTTGATCAA GAAGGAAAAG AAGAAGAGTA AGAAGGACAA GAAGGCCAAA 
GCTGGTCTGG AGAGCGGGGC CGAGCCTGGA GATGGGGACA GTGATACCAC CAAGAAGAAG 
AAGAAGAAGA AGAAAGCAAA AGAGGTAGAA TTGGTTTCTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354.1
CDS	103..1647
Translation	MADAEVIILPKKHKKKKERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYSESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE

Target ORF information:

RefSeq Version	NM_001363.5
Organism	Homo sapiens(human)
Definition	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363.5

ORF Insert Sequence:

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACGC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGAGTATGT TGACTACAGT 
GAGTCTGCCA AAAAAGAGGT GGTTGCTGAA GTGGTAAAAG CCCCGCAGGT AGTTGCCGAA 
GCAGCAAAAA CTGCGAAGCG GAAGCGAGAG AGTGAGAGTG AAAGTGACGA GACTCCTCCA 
GCAGCTCCTC AGTTGATCAA GAAGGAAAAG AAGAAGAGTA AGAAGGACAA GAAGGCCAAA 
GCTGGTCTGG AGAGCGGGGC CGAGCCTGGA GATGGGGACA GTGATACCAC CAAGAAGAAG 
AAGAAGAAGA AGAAAGCAAA AGAGGTAGAA TTGGTTTCTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DKC1 ( NM_001142463.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135935.1 Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108434
Clone ID Related Accession (Same CDS sequence)	NM_001142463.3
Accession Version	NM_001142463.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit DKC1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC109993.4. On May 31, 2019 this sequence version replaced NM_001142463.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.142250.1, SRR1660807.144951.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACGC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGATGAGTC TGCCAAAAAA 
GAGGTGGTTG CTGAAGTGGT AAAAGCCCCG CAGGTAGTTG CCGAAGCAGC AAAAACTGCG 
AAGCGGAAGC GAGAGAGTGA GAGTGAAAGT GACGAGACTC CTCCAGCAGC TCCTCAGTTG 
ATCAAGAAGG AAAAGAAGAA GAGTAAGAAG GACAAGAAGG CCAAAGCTGG TCTGGAGAGC 
GGGGCCGAGC CTGGAGATGG GGACAGTGAT ACCACCAAGA AGAAGAAGAA GAAGAAGAAA 
GCAAAAGAGG TAGAATTGGT TTCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135935.1
CDS	103..1632
Translation	MADAEVIILPKKHKKKKERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQDESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE

Target ORF information:

RefSeq Version	NM_001142463.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142463.3

ORF Insert Sequence:

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACGC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGATGAGTC TGCCAAAAAA 
GAGGTGGTTG CTGAAGTGGT AAAAGCCCCG CAGGTAGTTG CCGAAGCAGC AAAAACTGCG 
AAGCGGAAGC GAGAGAGTGA GAGTGAAAGT GACGAGACTC CTCCAGCAGC TCCTCAGTTG 
ATCAAGAAGG AAAAGAAGAA GAGTAAGAAG GACAAGAAGG CCAAAGCTGG TCTGGAGAGC 
GGGGCCGAGC CTGGAGATGG GGACAGTGAT ACCACCAAGA AGAAGAAGAA GAAGAAGAAA 
GCAAAAGAGG TAGAATTGGT TTCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DKC1 ( NM_001142463.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135935.1 Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23916
Clone ID Related Accession (Same CDS sequence)	NM_001142463.2
Accession Version	NM_001142463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551542400000
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit DKC1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY050100.1, BC009928.2, AK307672.1 and CD367400.1. On Jan 3, 2014 this sequence version replaced NM_001142463.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.142250.1, SRR1660807.261512.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACTC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGATGAGTC TGCCAAAAAA 
GAGGTGGTTG CTGAAGTGGT AAAAGCCCCG CAGGTAGTTG CCGAAGCAGC AAAAACTGCG 
AAGCGGAAGC GAGAGAGTGA GAGTGAAAGT GACGAGACTC CTCCAGCAGC TCCTCAGTTG 
ATCAAGAAGG AAAAGAAGAA GAGTAAGAAG GACAAGAAGG CCAAAGCTGG TCTGGAGAGC 
GGGGCCGAGC CTGGAGATGG GGACAGTGAT ACCACCAAGA AGAAGAAGAA GAAGAAGAAA 
GCAAAAGAGG TAGAATTGGT TTCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135935.1
CDS	225..1754
Translation	MADAEVIILPKKHKKKKERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQDESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE

Target ORF information:

RefSeq Version	NM_001142463.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142463.2

ORF Insert Sequence:

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACTC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGATGAGTC TGCCAAAAAA 
GAGGTGGTTG CTGAAGTGGT AAAAGCCCCG CAGGTAGTTG CCGAAGCAGC AAAAACTGCG 
AAGCGGAAGC GAGAGAGTGA GAGTGAAAGT GACGAGACTC CTCCAGCAGC TCCTCAGTTG 
ATCAAGAAGG AAAAGAAGAA GAGTAAGAAG GACAAGAAGG CCAAAGCTGG TCTGGAGAGC 
GGGGCCGAGC CTGGAGATGG GGACAGTGAT ACCACCAAGA AGAAGAAGAA GAAGAAGAAA 
GCAAAAGAGG TAGAATTGGT TTCTGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DKC1 ( NM_001288747.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275676.1 Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 3, mRNA.

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CloneID	OHu23474
Clone ID Related Accession (Same CDS sequence)	NM_001288747.1
Accession Version	NM_001288747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit DKC1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY050100.1, BC009928.2, JF279874.1 and CD367400.1. Transcript Variant: This variant (3) uses an alternate 3' exon structure, which results in an early stop codon, compared to variant 1. The resulting protein (isoform 3) has a distinct C-terminus, compared to isoform 1 (PMID: 21820037). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.163894.1, SRR1803614.265707.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 21820037, 24219293 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACTC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGAGTATGT TGACTACAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275676.1
CDS	225..1487
Translation	MADAEVIILPKKHKKKKERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYR

Target ORF information:

RefSeq Version	NM_001288747.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288747.1

ORF Insert Sequence:

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACTC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGAGTATGT TGACTACAGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DKC1 ( NM_001363.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354.1 Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 1, mRNA.

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CloneID	OHu23856
Clone ID Related Accession (Same CDS sequence)	NM_001363.4
Accession Version	NM_001363.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	H/ACA ribonucleoprotein complex subunit 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY050100.1, BC009928.2, AK225077.1 and CD367400.1. On Jan 3, 2014 this sequence version replaced NM_001363.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009928.2, SRR1660803.42859.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACTC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGAGTATGT TGACTACAGT 
GAGTCTGCCA AAAAAGAGGT GGTTGCTGAA GTGGTAAAAG CCCCGCAGGT AGTTGCCGAA 
GCAGCAAAAA CTGCGAAGCG GAAGCGAGAG AGTGAGAGTG AAAGTGACGA GACTCCTCCA 
GCAGCTCCTC AGTTGATCAA GAAGGAAAAG AAGAAGAGTA AGAAGGACAA GAAGGCCAAA 
GCTGGTCTGG AGAGCGGGGC CGAGCCTGGA GATGGGGACA GTGATACCAC CAAGAAGAAG 
AAGAAGAAGA AGAAAGCAAA AGAGGTAGAA TTGGTTTCTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354.1
CDS	225..1769
Translation	MADAEVIILPKKHKKKKERKSLPEEDVAEIQHAEEFLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYTPLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYSESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE

Target ORF information:

RefSeq Version	NM_001363.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dyskerin pseudouridine synthase 1 (DKC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363.4

ORF Insert Sequence:

ATGGCGGATG CGGAAGTAAT TATTTTGCCA AAGAAACATA AGAAGAAAAA GGAGCGGAAG 
TCATTGCCAG AAGAAGATGT AGCCGAAATA CAACACGCTG AAGAATTTCT TATCAAACCT 
GAATCCAAAG TTGCTAAGTT GGACACGTCT CAGTGGCCCC TTTTGCTAAA GAATTTTGAT 
AAGCTGAATG TAAGGACAAC ACACTATACA CCTCTTGCAT GTGGTTCAAA TCCTCTGAAG 
AGAGAGATTG GGGACTATAT CAGGACAGGT TTCATTAATC TTGACAAGCC CTCTAACCCC 
TCTTCCCATG AGGTGGTAGC CTGGATTCGA CGGATACTTC GGGTGGAGAA GACAGGGCAC 
AGTGGTACTC TGGATCCCAA GGTGACTGGT TGTTTAATCG TGTGCATAGA ACGAGCCACT 
CGCTTGGTGA AGTCACAACA GAGTGCAGGC AAAGAGTATG TGGGGATTGT CCGGCTGCAC 
AATGCTATTG AAGGGGGGAC CCAGCTTTCT AGGGCCCTAG AAACTCTGAC AGGTGCCTTA 
TTCCAGCGAC CCCCACTTAT TGCTGCAGTA AAGAGGCAGC TCCGAGTGAG GACCATCTAC 
GAGAGCAAAA TGATTGAATA CGATCCTGAA AGAAGATTAG GAATCTTTTG GGTGAGTTGT 
GAGGCTGGCA CCTACATTCG GACATTATGT GTGCACCTTG GTTTGTTATT GGGAGTTGGT 
GGTCAGATGC AGGAGCTTCG GAGGGTTCGT TCTGGAGTCA TGAGTGAAAA GGACCACATG 
GTGACAATGC ATGATGTGCT TGATGCTCAG TGGCTGTATG ATAACCACAA GGATGAGAGT 
TACCTGCGGC GAGTTGTTTA CCCTTTGGAA AAGCTGTTGA CATCTCATAA ACGGCTGGTT 
ATGAAAGACA GTGCAGTAAA TGCCATCTGC TATGGGGCCA AGATTATGCT TCCAGGTGTT 
CTTCGATATG AGGACGGCAT TGAGGTCAAT CAGGAGATTG TGGTTATCAC CACCAAAGGA 
GAAGCAATCT GCATGGCTAT TGCATTAATG ACCACAGCGG TCATCTCTAC CTGCGACCAT 
GGTATAGTAG CCAAGATCAA GAGAGTGATC ATGGAGAGAG ACACTTACCC TCGGAAGTGG 
GGTTTAGGTC CAAAGGCAAG TCAGAAGAAG CTGATGATCA AGCAGGGCCT TCTGGACAAG 
CATGGGAAGC CCACAGACAG CACACCTGCC ACCTGGAAGC AGGAGTATGT TGACTACAGT 
GAGTCTGCCA AAAAAGAGGT GGTTGCTGAA GTGGTAAAAG CCCCGCAGGT AGTTGCCGAA 
GCAGCAAAAA CTGCGAAGCG GAAGCGAGAG AGTGAGAGTG AAAGTGACGA GACTCCTCCA 
GCAGCTCCTC AGTTGATCAA GAAGGAAAAG AAGAAGAGTA AGAAGGACAA GAAGGCCAAA 
GCTGGTCTGG AGAGCGGGGC CGAGCCTGGA GATGGGGACA GTGATACCAC CAAGAAGAAG 
AAGAAGAAGA AGAAAGCAAA AGAGGTAGAA TTGGTTTCTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation.
Nucleic acids research47(10)5368-5380(2019 06)
MacNeil DE,Lambert-Lanteigne P,Autexier C

Characterization of an X-chromosome-linked telomere biology disorder in females with DKC1 mutation.
Leukemia33(1)275-278(2019 01)
Hirvonen EAM,Peuhkuri S,Norberg A,Degerman S,Hannula-Jouppi K,Välimaa H,Kilpivaara O,Wartiovaara-Kautto U

DKC1 serves as a potential prognostic biomarker for human clear cell renal cell carcinoma and promotes its proliferation, migration and invasion via the NF‑κB pathway.
Oncology reports40(2)968-978(2018 Aug)
Zhang M,Pan Y,Jiang R,Hou P,Shan H,Chen F,Jiang T,Bai J,Zheng J

Dyskeratosis congenita with a novel genetic variant in the DKC1 gene: a case report.
BMC medical genetics19(1)85(2018 05)
Ratnasamy V,Navaneethakrishnan S,Sirisena ND,Grüning NM,Brandau O,Thirunavukarasu K,Dagnall CL,McReynolds LJ,Savage SA,Dissanayake VHW

Cryo-EM structure of substrate-bound human telomerase holoenzyme.
Nature557(7704)190-195(2018 05)
Nguyen THD,Tam J,Wu RA,Greber BJ,Toso D,Nogales E,Collins K

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DKC1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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