CHRNA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNA1
Entrez Gene ID	1134
Full Name	cholinergic receptor nicotinic alpha 1 subunit
Synonyms	ACHRA,ACHRD,CHRNA,CMS1A,CMS1B,CMS2A,FCCMS,SCCMS
General protein information	Preferred Names cholinergic receptor nicotinic alpha 1 subunit Names acetylcholine receptor subunit alpha acetylcholine receptor, nicotinic, alpha 1 (muscle) cholinergic receptor, nicotinic alpha 1 cholinergic receptor, nicotinic, alpha 1 (muscle) cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) muscle nicotinic acetylcholine receptor nicotinic acetylcholine receptor alpha subunit nicotinic cholinergic receptor alpha 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q31.1
Summary	The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012].
Disorder MIM:	100690
Disorder Html:	Myasthenic syndrome, congenital, 1A, slow-channel, 601462 (3); Myasthenic syndrome, congenital, 1B, fast-channel, 608930 (3); Multiple pterygium syndrome, lethal type, 253290 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039523.3	NP_001034612.1	acetylcholine receptor subunit alpha isoform a precursor
XM_017003257.1	XP_016858746.1	acetylcholine receptor subunit alpha isoform X2
NM_000079.3	NP_000070.1	acetylcholine receptor subunit alpha isoform b precursor
XM_017003256.1	XP_016858745.1	acetylcholine receptor subunit alpha isoform X1
NM_001039523.2	NP_001034612.1	acetylcholine receptor subunit alpha isoform a precursor
NM_000079.4	NP_000070.1	acetylcholine receptor subunit alpha isoform b precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	chrna1	NP_571520.1
Gallus gallus (chicken)	CHRNA1	NP_990147.1
Pan troglodytes (chimpanzee)	CHRNA1	XP_003309365.1
Mus musculus (house mouse)	Chrna1	NP_031415.2
Homo sapiens (human)	CHRNA1	NP_001034612.1
Canis lupus familiaris (dog)	CHRNA1	NP_001003144.1
Macaca mulatta (Rhesus monkey)	CHRNA1	XP_001091711.1
Bos taurus (cattle)	CHRNA1	NP_788837.1
Rattus norvegicus (Norway rat)	Chrna1	NP_077811.1
Xenopus tropicalis (tropical clawed frog)	chrna1	XP_002934647.1

Gene Ontology
Bibliography

Related articles in PubMed

Evaluating the effect of nicotinic cholinergic receptor genes on the risk of nonsyndromic cleft lip with or without cleft palate.
Wang M, Liu D, Schwender H, Wang H, Wang P, Zhou Z, Li J, Wu T, Zhu H, Beaty TH
Oral diseases24(6)1068-1072(2018 Sep)

Juvenile-onset myasthenia gravis: autoantibody status, clinical characteristics and genetic polymorphisms.
Hong Y, Skeie GO, Zisimopoulou P, Karagiorgou K, Tzartos SJ, Gao X, Yue YX, Romi F, Zhang X, Li HF, Gilhus NE
Journal of neurology264(5)955-962(2017 May)

Effect of nicotinic acetylcholine receptor alpha 1 (nAChRα1) peptides on rabies virus infection in neuronal cells.
Sajjanar B, Saxena S, Bisht D, Singh AK, Manjunatha Reddy GB, Singh R, Singh RP, Kumar S
Neuropeptides5759-64(2016 Jun)

High Throughput Random Mutagenesis and Single Molecule Real Time Sequencing of the Muscle Nicotinic Acetylcholine Receptor.
Groot-Kormelink PJ, Ferrand S, Kelley N, Bill A, Freuler F, Imbert PE, Marelli A, Gerwin N, Sivilotti LG, Miraglia L, Orth AP, Oakeley EJ, Schopfer U, Siehler S
PloS one11(9)e0163129(2016)

Lateral diffusion, function, and expression of the slow channel congenital myasthenia syndrome αC418W nicotinic receptor mutation with changes in lipid raft components.
Oyola-Cintrón J, Caballero-Rivera D, Ballester L, Baéz-Pagán CA, Martínez HL, Vélez-Arroyo KP, Quesada O, Lasalde-Dominicci JA
The Journal of biological chemistry290(44)26790-800(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHRNA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16431	NM_000079.4 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu88974	XM_017003256.1 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu88975	XM_017003257.1 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu19640	NM_001039523.3 Latest version!	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$286.30 $409.00
View Old Accession Versions >>
OHu19640	NM_001039523.2	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$286.30 $409.00
OHu16431	NM_000079.3	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNA1 ( NM_000079.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000070.1 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16431
Clone ID Related Accession (Same CDS sequence)	NM_000079.3 , NM_000079.4
Accession Version	NM_000079.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558713600000
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit alpha isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, Y00762.1 and BC043196.2. On Jun 10, 2008 this sequence version replaced NM_000079.2. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), that is missing an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.167776.1, SRR1803617.81379.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG 
GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT 
CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT 
GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT 
CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG 
AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG 
GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG 
TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC 
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC 
CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG 
GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC 
GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC 
ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC 
CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC 
CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG 
ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG 
GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG 
TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG 
TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC 
GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000070.1
CDS	79..1452
Translation	MEPWPLLLLFSLCSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG

Target ORF information:

RefSeq Version	NM_000079.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000079.3

ORF Insert Sequence:

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG 
GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT 
CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT 
GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT 
CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG 
AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG 
GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG 
TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC 
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC 
CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG 
GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC 
GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC 
ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC 
CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC 
CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG 
ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG 
GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG 
TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG 
TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC 
GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA1 ( NM_000079.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000070.1 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16431
Clone ID Related Accession (Same CDS sequence)	NM_000079.3 , NM_000079.4
Accession Version	NM_000079.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1578067200000
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit alpha isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, Y00762.1 and BC043196.2. On Aug 31, 2019 this sequence version replaced NM_000079.3. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), that is missing an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315312.1, Y00762.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000348749.9/ ENSP00000261008.5 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG 
GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT 
CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT 
GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT 
CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG 
AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG 
GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG 
TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC 
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC 
CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG 
GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC 
GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC 
ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC 
CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC 
CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG 
ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG 
GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG 
TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG 
TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC 
GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000070.1
CDS	79..1452
Translation	MEPWPLLLLFSLCSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG

Target ORF information:

RefSeq Version	NM_000079.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000079.4

ORF Insert Sequence:

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGCAATGG 
GTGGATTACA ACCTAAAATG GAATCCAGAT GACTATGGCG GTGTGAAAAA AATTCACATT 
CCTTCAGAAA AGATCTGGCG CCCAGACCTT GTTCTCTATA ACAATGCAGA TGGTGACTTT 
GCTATTGTCA AGTTCACCAA AGTGCTCCTG CAGTACACTG GCCACATCAC GTGGACACCT 
CCAGCCATCT TTAAAAGCTA CTGTGAGATC ATCGTCACCC ACTTTCCCTT TGATGAACAG 
AACTGCAGCA TGAAGCTGGG CACCTGGACC TACGACGGCT CTGTCGTGGC CATCAACCCG 
GAAAGCGACC AGCCAGACCT GAGCAACTTC ATGGAGAGCG GGGAGTGGGT GATCAAGGAG 
TCCCGGGGCT GGAAGCACTC CGTGACCTAT TCCTGCTGCC CCGACACCCC CTACCTGGAC 
ATCACCTACC ACTTCGTCAT GCAGCGCCTG CCCCTCTACT TCATCGTCAA CGTCATCATC 
CCCTGCCTGC TCTTCTCCTT CTTAACTGGC CTGGTATTCT ACCTGCCCAC AGACTCAGGG 
GAGAAGATGA CTCTGAGCAT CTCTGTCTTA CTGTCTTTGA CTGTGTTCCT TCTGGTCATC 
GTGGAGCTGA TCCCCTCCAC GTCCAGTGCT GTGCCCTTGA TTGGAAAATA CATGCTGTTC 
ACCATGGTGT TCGTCATTGC CTCCATCATC ATCACTGTCA TCGTCATCAA CACACACCAC 
CGCTCACCCA GCACCCATGT CATGCCCAAC TGGGTGCGGA AGGTTTTTAT CGACACTATC 
CCAAATATCA TGTTTTTCTC CACAATGAAA AGACCATCCA GAGAAAAGCA AGACAAAAAG 
ATTTTTACAG AAGACATTGA TATCTCTGAC ATTTCTGGAA AGCCAGGGCC TCCACCCATG 
GGCTTCCACT CTCCCCTGAT CAAACACCCC GAGGTGAAAA GTGCCATCGA GGGCATCAAG 
TACATCGCAG AGACCATGAA GTCAGACCAG GAGTCTAACA ATGCGGCGGC AGAGTGGAAG 
TACGTTGCAA TGGTGATGGA CCACATACTC CTCGGAGTCT TCATGCTTGT TTGCATCATC 
GGAACCCTAG CCGTGTTTGC AGGTCGACTC ATTGAATTAA ATCAGCAAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA1 ( XM_017003256.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858745.1 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu88974
Clone ID Related Accession (Same CDS sequence)	XM_017003256.1
Accession Version	XM_017003256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA 
GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG 
GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT 
GTGCGTCTGA AACAGGGTGA CATGGTAGAT CTGCCACGCC CCAGCTGCGT GACTTTGGGA 
GTTCCTTTGT TTTCTCATCT GCAGAATGAG CAATGGGTGG ATTACAACCT AAAATGGAAT 
CCAGATGACT ATGGCGGTGT GAAAAAAATT CACATTCCTT CAGAAAAGAT CTGGCGCCCA 
GACCTTGTTC TCTATAACAA TGCAGATGGT GACTTTGCTA TTGTCAAGTT CACCAAAGTG 
CTCCTGCAGT ACACTGGCCA CATCACGTGG ACACCTCCAG CCATCTTTAA AAGCTACTGT 
GAGATCATCG TCACCCACTT TCCCTTTGAT GAACAGAACT GCAGCATGAA GCTGGGCACC 
TGGACCTACG ACGGCTCTGT CGTGGCCATC AACCCGGAAA GCGACCAGCC AGACCTGAGC 
AACTTCATGG AGAGCGGGGA GTGGGTGATC AAGGAGTCCC GGGGCTGGAA GCACTCCGTG 
ACCTATTCCT GCTGCCCCGA CACCCCCTAC CTGGACATCA CCTACCACTT CGTCATGCAG 
CGCCTGCCCC TCTACTTCAT CGTCAACGTC ATCATCCCCT GCCTGCTCTT CTCCTTCTTA 
ACTGGCCTGG TATTCTACCT GCCCACAGAC TCAGGGGAGA AGATGACTCT GAGCATCTCT 
GTCTTACTGT CTTTGACTGT GTTCCTTCTG GTCATCGTGG AGCTGATCCC CTCCACGTCC 
AGTGCTGTGC CCTTGATTGG AAAATACATG CTGTTCACCA TGGTGTTCGT CATTGCCTCC 
ATCATCATCA CTGTCATCGT CATCAACACA CACCACCGCT CACCCAGCAC CCATGTCATG 
CCCAACTGGG TGCGGAAGGT TTTTATCGAC ACTATCCCAA ATATCATGTT TTTCTCCACA 
ATGAAAAGAC CATCCAGAGA AAAGCAAGAC AAAAAGATTT TTACAGAAGA CATTGATATC 
TCTGACATTT CTGGAAAGCC AGGGCCTCCA CCCATGGGCT TCCACTCTCC CCTGATCAAA 
CACCCCGAGG TGAAAAGTGC CATCGAGGGC ATCAAGTACA TCGCAGAGAC CATGAAGTCA 
GACCAGGAGT CTAACAATGC GGCGGCAGAG TGGAAGTACG TTGCAATGGT GATGGACCAC 
ATACTCCTCG GAGTCTTCAT GCTTGTTTGC ATCATCGGAA CCCTAGCCGT GTTTGCAGGT 
CGACTCATTG AATTAAATCA GCAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858745.1
CDS	228..1697
Translation	MFMCLEGGEKNLTVLVSSAVSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQGDMVDLPRPSCVTLGVPLFSHLQNEQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG

Target ORF information:

RefSeq Version	XM_017003256.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003256.1

ORF Insert Sequence:

ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA 
GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG 
GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT 
GTGCGTCTGA AACAGGGTGA CATGGTAGAT CTGCCACGCC CCAGCTGCGT GACTTTGGGA 
GTTCCTTTGT TTTCTCATCT GCAGAATGAG CAATGGGTGG ATTACAACCT AAAATGGAAT 
CCAGATGACT ATGGCGGTGT GAAAAAAATT CACATTCCTT CAGAAAAGAT CTGGCGCCCA 
GACCTTGTTC TCTATAACAA TGCAGATGGT GACTTTGCTA TTGTCAAGTT CACCAAAGTG 
CTCCTGCAGT ACACTGGCCA CATCACGTGG ACACCTCCAG CCATCTTTAA AAGCTACTGT 
GAGATCATCG TCACCCACTT TCCCTTTGAT GAACAGAACT GCAGCATGAA GCTGGGCACC 
TGGACCTACG ACGGCTCTGT CGTGGCCATC AACCCGGAAA GCGACCAGCC AGACCTGAGC 
AACTTCATGG AGAGCGGGGA GTGGGTGATC AAGGAGTCCC GGGGCTGGAA GCACTCCGTG 
ACCTATTCCT GCTGCCCCGA CACCCCCTAC CTGGACATCA CCTACCACTT CGTCATGCAG 
CGCCTGCCCC TCTACTTCAT CGTCAACGTC ATCATCCCCT GCCTGCTCTT CTCCTTCTTA 
ACTGGCCTGG TATTCTACCT GCCCACAGAC TCAGGGGAGA AGATGACTCT GAGCATCTCT 
GTCTTACTGT CTTTGACTGT GTTCCTTCTG GTCATCGTGG AGCTGATCCC CTCCACGTCC 
AGTGCTGTGC CCTTGATTGG AAAATACATG CTGTTCACCA TGGTGTTCGT CATTGCCTCC 
ATCATCATCA CTGTCATCGT CATCAACACA CACCACCGCT CACCCAGCAC CCATGTCATG 
CCCAACTGGG TGCGGAAGGT TTTTATCGAC ACTATCCCAA ATATCATGTT TTTCTCCACA 
ATGAAAAGAC CATCCAGAGA AAAGCAAGAC AAAAAGATTT TTACAGAAGA CATTGATATC 
TCTGACATTT CTGGAAAGCC AGGGCCTCCA CCCATGGGCT TCCACTCTCC CCTGATCAAA 
CACCCCGAGG TGAAAAGTGC CATCGAGGGC ATCAAGTACA TCGCAGAGAC CATGAAGTCA 
GACCAGGAGT CTAACAATGC GGCGGCAGAG TGGAAGTACG TTGCAATGGT GATGGACCAC 
ATACTCCTCG GAGTCTTCAT GCTTGTTTGC ATCATCGGAA CCCTAGCCGT GTTTGCAGGT 
CGACTCATTG AATTAAATCA GCAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA1 ( XM_017003257.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858746.1 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X2, mRNA.

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CloneID	OHu88975
Clone ID Related Accession (Same CDS sequence)	XM_017003257.1
Accession Version	XM_017003257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA 
GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG 
GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT 
GTGCGTCTGA AACAGCAATG GGTGGATTAC AACCTAAAAT GGAATCCAGA TGACTATGGC 
GGTGTGAAAA AAATTCACAT TCCTTCAGAA AAGATCTGGC GCCCAGACCT TGTTCTCTAT 
AACAATGCAG ATGGTGACTT TGCTATTGTC AAGTTCACCA AAGTGCTCCT GCAGTACACT 
GGCCACATCA CGTGGACACC TCCAGCCATC TTTAAAAGCT ACTGTGAGAT CATCGTCACC 
CACTTTCCCT TTGATGAACA GAACTGCAGC ATGAAGCTGG GCACCTGGAC CTACGACGGC 
TCTGTCGTGG CCATCAACCC GGAAAGCGAC CAGCCAGACC TGAGCAACTT CATGGAGAGC 
GGGGAGTGGG TGATCAAGGA GTCCCGGGGC TGGAAGCACT CCGTGACCTA TTCCTGCTGC 
CCCGACACCC CCTACCTGGA CATCACCTAC CACTTCGTCA TGCAGCGCCT GCCCCTCTAC 
TTCATCGTCA ACGTCATCAT CCCCTGCCTG CTCTTCTCCT TCTTAACTGG CCTGGTATTC 
TACCTGCCCA CAGACTCAGG GGAGAAGATG ACTCTGAGCA TCTCTGTCTT ACTGTCTTTG 
ACTGTGTTCC TTCTGGTCAT CGTGGAGCTG ATCCCCTCCA CGTCCAGTGC TGTGCCCTTG 
ATTGGAAAAT ACATGCTGTT CACCATGGTG TTCGTCATTG CCTCCATCAT CATCACTGTC 
ATCGTCATCA ACACACACCA CCGCTCACCC AGCACCCATG TCATGCCCAA CTGGGTGCGG 
AAGGTTTTTA TCGACACTAT CCCAAATATC ATGTTTTTCT CCACAATGAA AAGACCATCC 
AGAGAAAAGC AAGACAAAAA GATTTTTACA GAAGACATTG ATATCTCTGA CATTTCTGGA 
AAGCCAGGGC CTCCACCCAT GGGCTTCCAC TCTCCCCTGA TCAAACACCC CGAGGTGAAA 
AGTGCCATCG AGGGCATCAA GTACATCGCA GAGACCATGA AGTCAGACCA GGAGTCTAAC 
AATGCGGCGG CAGAGTGGAA GTACGTTGCA ATGGTGATGG ACCACATACT CCTCGGAGTC 
TTCATGCTTG TTTGCATCAT CGGAACCCTA GCCGTGTTTG CAGGTCGACT CATTGAATTA 
AATCAGCAAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858746.1
CDS	225..1619
Translation	MFMCLEGGEKNLTVLVSSAVSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG

Target ORF information:

RefSeq Version	XM_017003257.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003257.1

ORF Insert Sequence:

ATGTTCATGT GTTTGGAGGG TGGGGAGAAG AATCTGACAG TGTTGGTGTC ATCTGCTGTC 
TCAGCTGGCC TCGTCCTGGG CTCCGAACAT GAGACCCGTC TGGTGGCAAA GCTATTTAAA 
GACTACAGCA GCGTGGTGCG GCCAGTGGAA GACCACCGCC AGGTCGTGGA GGTCACCGTG 
GGCCTGCAGC TGATACAGCT CATCAATGTG GATGAAGTAA ATCAGATCGT GACAACCAAT 
GTGCGTCTGA AACAGCAATG GGTGGATTAC AACCTAAAAT GGAATCCAGA TGACTATGGC 
GGTGTGAAAA AAATTCACAT TCCTTCAGAA AAGATCTGGC GCCCAGACCT TGTTCTCTAT 
AACAATGCAG ATGGTGACTT TGCTATTGTC AAGTTCACCA AAGTGCTCCT GCAGTACACT 
GGCCACATCA CGTGGACACC TCCAGCCATC TTTAAAAGCT ACTGTGAGAT CATCGTCACC 
CACTTTCCCT TTGATGAACA GAACTGCAGC ATGAAGCTGG GCACCTGGAC CTACGACGGC 
TCTGTCGTGG CCATCAACCC GGAAAGCGAC CAGCCAGACC TGAGCAACTT CATGGAGAGC 
GGGGAGTGGG TGATCAAGGA GTCCCGGGGC TGGAAGCACT CCGTGACCTA TTCCTGCTGC 
CCCGACACCC CCTACCTGGA CATCACCTAC CACTTCGTCA TGCAGCGCCT GCCCCTCTAC 
TTCATCGTCA ACGTCATCAT CCCCTGCCTG CTCTTCTCCT TCTTAACTGG CCTGGTATTC 
TACCTGCCCA CAGACTCAGG GGAGAAGATG ACTCTGAGCA TCTCTGTCTT ACTGTCTTTG 
ACTGTGTTCC TTCTGGTCAT CGTGGAGCTG ATCCCCTCCA CGTCCAGTGC TGTGCCCTTG 
ATTGGAAAAT ACATGCTGTT CACCATGGTG TTCGTCATTG CCTCCATCAT CATCACTGTC 
ATCGTCATCA ACACACACCA CCGCTCACCC AGCACCCATG TCATGCCCAA CTGGGTGCGG 
AAGGTTTTTA TCGACACTAT CCCAAATATC ATGTTTTTCT CCACAATGAA AAGACCATCC 
AGAGAAAAGC AAGACAAAAA GATTTTTACA GAAGACATTG ATATCTCTGA CATTTCTGGA 
AAGCCAGGGC CTCCACCCAT GGGCTTCCAC TCTCCCCTGA TCAAACACCC CGAGGTGAAA 
AGTGCCATCG AGGGCATCAA GTACATCGCA GAGACCATGA AGTCAGACCA GGAGTCTAAC 
AATGCGGCGG CAGAGTGGAA GTACGTTGCA ATGGTGATGG ACCACATACT CCTCGGAGTC 
TTCATGCTTG TTTGCATCAT CGGAACCCTA GCCGTGTTTG CAGGTCGACT CATTGAATTA 
AATCAGCAAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA1 ( NM_001039523.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034612.1 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.

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CloneID	OHu19640
Clone ID Related Accession (Same CDS sequence)	NM_001039523.3 , NM_001039523.2
Accession Version	NM_001039523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit alpha isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, CD013889.1 and BC043196.2. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001039523.2. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.102475.1, AK299445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMN03267754 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC 
ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG 
CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG 
AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT 
GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC 
ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT 
CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC 
GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG 
TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC 
ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC 
GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG 
CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG 
TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA 
AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC 
ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT 
TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA 
AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA 
GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC 
ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG 
GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG 
CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG 
CAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034612.1
CDS	79..1527
Translation	MEPWPLLLLFSLCSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQGDMVDLPRPSCVTLGVPLFSHLQNEQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG

Target ORF information:

RefSeq Version	NM_001039523.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039523.3

ORF Insert Sequence:

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC 
ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG 
CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG 
AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT 
GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC 
ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT 
CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC 
GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG 
TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC 
ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC 
GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG 
CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG 
TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA 
AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC 
ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT 
TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA 
AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA 
GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC 
ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG 
GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG 
CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG 
CAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNA1 ( NM_001039523.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034612.1 Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19640
Clone ID Related Accession (Same CDS sequence)	NM_001039523.3 , NM_001039523.2
Accession Version	NM_001039523.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550592000000
Organism	Homo sapiens(human)
Product	acetylcholine receptor subunit alpha isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315312.1, CD013889.1 and BC043196.2. This sequence is a reference standard in the RefSeqGene project. On Jun 10, 2008 this sequence version replaced NM_001039523.1. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.102475.1, AK299445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMN03267754 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC 
ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG 
CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG 
AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT 
GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC 
ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT 
CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC 
GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG 
TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC 
ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC 
GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG 
CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG 
TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA 
AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC 
ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT 
TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA 
AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA 
GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC 
ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG 
GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG 
CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG 
CAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034612.1
CDS	79..1527
Translation	MEPWPLLLLFSLCSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQGDMVDLPRPSCVTLGVPLFSHLQNEQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWKYVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG

Target ORF information:

RefSeq Version	NM_001039523.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor nicotinic alpha 1 subunit (CHRNA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039523.2

ORF Insert Sequence:

ATGGAGCCCT GGCCTCTCCT CCTGCTCTTT AGCCTTTGCT CAGCTGGCCT CGTCCTGGGC 
TCCGAACATG AGACCCGTCT GGTGGCAAAG CTATTTAAAG ACTACAGCAG CGTGGTGCGG 
CCAGTGGAAG ACCACCGCCA GGTCGTGGAG GTCACCGTGG GCCTGCAGCT GATACAGCTC 
ATCAATGTGG ATGAAGTAAA TCAGATCGTG ACAACCAATG TGCGTCTGAA ACAGGGTGAC 
ATGGTAGATC TGCCACGCCC CAGCTGCGTG ACTTTGGGAG TTCCTTTGTT TTCTCATCTG 
CAGAATGAGC AATGGGTGGA TTACAACCTA AAATGGAATC CAGATGACTA TGGCGGTGTG 
AAAAAAATTC ACATTCCTTC AGAAAAGATC TGGCGCCCAG ACCTTGTTCT CTATAACAAT 
GCAGATGGTG ACTTTGCTAT TGTCAAGTTC ACCAAAGTGC TCCTGCAGTA CACTGGCCAC 
ATCACGTGGA CACCTCCAGC CATCTTTAAA AGCTACTGTG AGATCATCGT CACCCACTTT 
CCCTTTGATG AACAGAACTG CAGCATGAAG CTGGGCACCT GGACCTACGA CGGCTCTGTC 
GTGGCCATCA ACCCGGAAAG CGACCAGCCA GACCTGAGCA ACTTCATGGA GAGCGGGGAG 
TGGGTGATCA AGGAGTCCCG GGGCTGGAAG CACTCCGTGA CCTATTCCTG CTGCCCCGAC 
ACCCCCTACC TGGACATCAC CTACCACTTC GTCATGCAGC GCCTGCCCCT CTACTTCATC 
GTCAACGTCA TCATCCCCTG CCTGCTCTTC TCCTTCTTAA CTGGCCTGGT ATTCTACCTG 
CCCACAGACT CAGGGGAGAA GATGACTCTG AGCATCTCTG TCTTACTGTC TTTGACTGTG 
TTCCTTCTGG TCATCGTGGA GCTGATCCCC TCCACGTCCA GTGCTGTGCC CTTGATTGGA 
AAATACATGC TGTTCACCAT GGTGTTCGTC ATTGCCTCCA TCATCATCAC TGTCATCGTC 
ATCAACACAC ACCACCGCTC ACCCAGCACC CATGTCATGC CCAACTGGGT GCGGAAGGTT 
TTTATCGACA CTATCCCAAA TATCATGTTT TTCTCCACAA TGAAAAGACC ATCCAGAGAA 
AAGCAAGACA AAAAGATTTT TACAGAAGAC ATTGATATCT CTGACATTTC TGGAAAGCCA 
GGGCCTCCAC CCATGGGCTT CCACTCTCCC CTGATCAAAC ACCCCGAGGT GAAAAGTGCC 
ATCGAGGGCA TCAAGTACAT CGCAGAGACC ATGAAGTCAG ACCAGGAGTC TAACAATGCG 
GCGGCAGAGT GGAAGTACGT TGCAATGGTG ATGGACCACA TACTCCTCGG AGTCTTCATG 
CTTGTTTGCA TCATCGGAAC CCTAGCCGTG TTTGCAGGTC GACTCATTGA ATTAAATCAG 
CAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluating the effect of nicotinic cholinergic receptor genes on the risk of nonsyndromic cleft lip with or without cleft palate.
Oral diseases24(6)1068-1072(2018 Sep)
Wang M,Liu D,Schwender H,Wang H,Wang P,Zhou Z,Li J,Wu T,Zhu H,Beaty TH

Juvenile-onset myasthenia gravis: autoantibody status, clinical characteristics and genetic polymorphisms.
Journal of neurology264(5)955-962(2017 May)
Hong Y,Skeie GO,Zisimopoulou P,Karagiorgou K,Tzartos SJ,Gao X,Yue YX,Romi F,Zhang X,Li HF,Gilhus NE

Effect of nicotinic acetylcholine receptor alpha 1 (nAChRα1) peptides on rabies virus infection in neuronal cells.
Neuropeptides5759-64(2016 Jun)
Sajjanar B,Saxena S,Bisht D,Singh AK,Manjunatha Reddy GB,Singh R,Singh RP,Kumar S

High Throughput Random Mutagenesis and Single Molecule Real Time Sequencing of the Muscle Nicotinic Acetylcholine Receptor.
PloS one11(9)e0163129(2016)
Groot-Kormelink PJ,Ferrand S,Kelley N,Bill A,Freuler F,Imbert PE,Marelli A,Gerwin N,Sivilotti LG,Miraglia L,Orth AP,Oakeley EJ,Schopfer U,Siehler S

Lateral diffusion, function, and expression of the slow channel congenital myasthenia syndrome αC418W nicotinic receptor mutation with changes in lipid raft components.
The Journal of biological chemistry290(44)26790-800(2015 Oct)
Oyola-Cintrón J,Caballero-Rivera D,Ballester L,Baéz-Pagán CA,Martínez HL,Vélez-Arroyo KP,Quesada O,Lasalde-Dominicci JA

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CHRNA1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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