EMG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMG1
Entrez Gene ID	10436
Full Name	EMG1, N1-specific pseudouridine methyltransferase
Synonyms	C2F,Grcc2f,NEP1
General protein information	Preferred Names EMG1, N1-specific pseudouridine methyltransferase Names ribosomal RNA small subunit methyltransferase NEP1 18S rRNA (pseudouridine(1248)-N1)-methyltransferase 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1 18S rRNA Psi1248 methyltransferase EMG1 nucleolar protein homolog essential for mitotic growth 1 nucleolar essential protein 1 ribosome biogenesis protein NEP1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	This gene encodes an essential, conserved eukaryotic protein that methylates pseudouridine in 18S rRNA. The related protein in yeast is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016].
Disorder MIM:	611531
Disorder Html:	Bowen-Conradi syndrome, 211180 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320049.1	NP_001306978.1	ribosomal RNA small subunit methyltransferase NEP1 isoform 2
NM_001320049.2	NP_001306978.1	ribosomal RNA small subunit methyltransferase NEP1 isoform 2
NM_006331.7	NP_006322.4	ribosomal RNA small subunit methyltransferase NEP1 isoform 1
NM_006331.8	NP_006322.4	ribosomal RNA small subunit methyltransferase NEP1 isoform 1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR686C	NP_986234.1
Magnaporthe oryzae (rice blast fungus)	MGG_03587	XP_003716322.1
Oryza sativa (rice)	Os02g0290400	NP_001046590.1
Canis lupus familiaris (dog)	EMG1	XP_853373.1
Mus musculus (house mouse)	Emg1	NP_038564.1
Saccharomyces cerevisiae (baker's yeast)	EMG1	NP_013287.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002808	XP_312105.2
Neurospora crassa	NCU02428	XP_959433.2
Schizosaccharomyces pombe (fission yeast)	mra1	NP_593671.1
Rattus norvegicus (Norway rat)	Emg1	NP_001101358.2
Drosophila melanogaster (fruit fly)	CG3527	NP_572170.1
Arabidopsis thaliana (thale cress)	AT3G57000	NP_191259.1
Pan troglodytes (chimpanzee)	EMG1	XP_003313518.1
Kluyveromyces lactis	KLLA0C01914g	XP_452281.1
Homo sapiens (human)	EMG1	NP_006322.4
Gallus gallus (chicken)	EMG1	NP_001264998.1
Danio rerio (zebrafish)	zgc:136360	NP_001035427.1
Caenorhabditis elegans (roundworm)	CELE_Y39A1A.14	NP_499349.1
Macaca mulatta (Rhesus monkey)	EMG1	XP_001118315.2
Bos taurus (cattle)	EMG1	NP_001039662.2
Xenopus tropicalis (tropical clawed frog)	emg1	XP_002941256.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment.
Warda AS, Freytag B, Haag S, Sloan KE, Görlich D, Bohnsack MT
Human molecular genetics25(24)5353-5364(2016 12)

The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.
Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kötter P, Engels JW, Heckel A, Karas M, Entian KD, Wöhnert J
Nucleic acids research38(7)2387-98(2010 Apr)

Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome.
Armistead J, Khatkar S, Meyer B, Mark BL, Patel N, Coghlan G, Lamont RE, Liu S, Wiechert J, Cattini PA, Koetter P, Wrogemann K, Greenberg CR, Entian KD, Zelinski T, Triggs-Raine B
American journal of human genetics84(6)728-39(2009 Jun)

A locus for Bowen-Conradi syndrome maps to chromosome region 12p13.3.
Lamont RE, Loredo-Osti J, Roslin NM, Mauthe J, Coghlan G, Nylen E, Frappier D, Innes AM, Lemire EG, Lowry RB, Greenberg CR, Triggs-Raine BL, Morgan K, Wrogemann K, Fujiwara TM, Zelinski T
American journal of medical genetics. Part A132A(2)136-43(2005 Jan)

The small-subunit processome is a ribosome assembly intermediate.
Bernstein KA, Gallagher JE, Mitchell BM, Granneman S, Baserga SJ
Eukaryotic cell3(6)1619-26(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EMG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu64014	NM_001320049.2 Latest version!	Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu18908	NM_006331.8 Latest version!	Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu64014	NM_001320049.1	Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu18908	NM_006331.7	Homo sapiens EMG1, N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMG1 ( NM_001320049.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306978.1 Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu64014
Clone ID Related Accession (Same CDS sequence)	NM_001320049.1 , NM_001320049.2
Accession Version	NM_001320049.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548518400000
Organism	Homo sapiens(human)
Product	ribosomal RNA small subunit methyltransferase NEP1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC403138.1, BC055314.1, BP315329.1 and AI394112.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.56926.1, SRR1163658.275050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTCAGTGTG 
GAGTATACAG AGAAGATGGT GTCCATCAGT AACTACCCCC TTTCTGCTGC CCTCACCTGT 
GCAAAACTTA CCACAGCCTT TGAGGAAGTA TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306978.1
CDS	144..728
Translation	MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI

Target ORF information:

RefSeq Version	NM_001320049.1
Organism	Homo sapiens(human)
Definition	Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320049.1

ORF Insert Sequence:

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTCAGTGTG 
GAGTATACAG AGAAGATGGT GTCCATCAGT AACTACCCCC TTTCTGCTGC CCTCACCTGT 
GCAAAACTTA CCACAGCCTT TGAGGAAGTA TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMG1 ( NM_001320049.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001306978.1 Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu64014
Clone ID Related Accession (Same CDS sequence)	NM_001320049.1 , NM_001320049.2
Accession Version	NM_001320049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559145600000
Organism	Homo sapiens(human)
Product	ribosomal RNA small subunit methyltransferase NEP1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC403138.1, BC055314.1, BP315329.1, AI394112.1 and AC006512.13. On May 31, 2019 this sequence version replaced NM_001320049.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BP315329.1, BG777740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTCAGTGTG 
GAGTATACAG AGAAGATGGT GTCCATCAGT AACTACCCCC TTTCTGCTGC CCTCACCTGT 
GCAAAACTTA CCACAGCCTT TGAGGAAGTA TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001306978.1
CDS	12..596
Translation	MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI

Target ORF information:

RefSeq Version	NM_001320049.2
Organism	Homo sapiens(human)
Definition	Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320049.2

ORF Insert Sequence:

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTCAGTGTG 
GAGTATACAG AGAAGATGGT GTCCATCAGT AACTACCCCC TTTCTGCTGC CCTCACCTGT 
GCAAAACTTA CCACAGCCTT TGAGGAAGTA TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMG1 ( NM_006331.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_006322.4 Homo sapiens EMG1, N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18908
Clone ID Related Accession (Same CDS sequence)	NM_006331.7 , NM_006331.8
Accession Version	NM_006331.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	ribosomal RNA small subunit methyltransferase NEP1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC403138.1, BC055314.1 and AI394112.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced NM_006331.6. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163655.543492.1, SRR1803613.234521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTAATTAAG 
AATCCAGTAT CAGATCACTT TCCAGTTGGA TGTATGAAAG TTGGCACTTC TTTTTCCATC 
CCGGTTGTCA GTGATGTGCG TGAGCTGGTG CCCAGCAGTG ATCCTATTGT TTTTGTGGTA 
GGGGCCTTTG CCCATGGCAA GGTCAGTGTG GAGTATACAG AGAAGATGGT GTCCATCAGT 
AACTACCCCC TTTCTGCTGC CCTCACCTGT GCAAAACTTA CCACAGCCTT TGAGGAAGTA 
TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006322.4
CDS	144..878
Translation	MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI

Target ORF information:

RefSeq Version	NM_006331.7
Organism	Homo sapiens(human)
Definition	Homo sapiens EMG1, N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006331.7

ORF Insert Sequence:

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTAATTAAG 
AATCCAGTAT CAGATCACTT TCCAGTTGGA TGTATGAAAG TTGGCACTTC TTTTTCCATC 
CCGGTTGTCA GTGATGTGCG TGAGCTGGTG CCCAGCAGTG ATCCTATTGT TTTTGTGGTA 
GGGGCCTTTG CCCATGGCAA GGTCAGTGTG GAGTATACAG AGAAGATGGT GTCCATCAGT 
AACTACCCCC TTTCTGCTGC CCTCACCTGT GCAAAACTTA CCACAGCCTT TGAGGAAGTA 
TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMG1 ( NM_006331.8 ) cDNA ORF clone, Homo sapiens(human) -> NP_006322.4 Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18908
Clone ID Related Accession (Same CDS sequence)	NM_006331.7 , NM_006331.8
Accession Version	NM_006331.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	ribosomal RNA small subunit methyltransferase NEP1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC403138.1, BC055314.1, AI394112.1 and AC006512.13. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006331.7. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803612.200170.1, SRR1803616.79428.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000599672.6/ ENSP00000470560.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTAATTAAG 
AATCCAGTAT CAGATCACTT TCCAGTTGGA TGTATGAAAG TTGGCACTTC TTTTTCCATC 
CCGGTTGTCA GTGATGTGCG TGAGCTGGTG CCCAGCAGTG ATCCTATTGT TTTTGTGGTA 
GGGGCCTTTG CCCATGGCAA GGTCAGTGTG GAGTATACAG AGAAGATGGT GTCCATCAGT 
AACTACCCCC TTTCTGCTGC CCTCACCTGT GCAAAACTTA CCACAGCCTT TGAGGAAGTA 
TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006322.4
CDS	12..746
Translation	MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI

Target ORF information:

RefSeq Version	NM_006331.8
Organism	Homo sapiens(human)
Definition	Homo sapiens EMG1 N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006331.8

ORF Insert Sequence:

ATGGCCGCGC CCAGTGATGG ATTCAAGCCT CGTGAACGAA GCGGTGGGGA GCAGGCACAG 
GACTGGGATG CTCTGCCACC CAAGCGGCCC CGACTAGGGG CAGGAAACAA GATCGGAGGC 
CGTAGGCTTA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAGACA 
TATGAGCTAC TCAACTGTGA CAAGCACAAG TCTATATTGT TGAAGAATGG ACGGGACCCT 
GGGGAAGCGC GGCCAGATAT CACCCACCAG AGTTTGCTGA TGCTGATGGA TAGTCCCCTG 
AACCGAGCTG GCTTGCTACA GGTTTATATC CATACACAGA AGAATGTTCT GATTGAAGTG 
AATCCCCAGA CCCGAATTCC CAGAACCTTT GACCGCTTTT GTGGCCTCAT GGTTCAACTT 
TTACACAAGC TCAGTGTTCG AGCAGCTGAT GGCCCCCAGA AGCTTTTGAA GGTAATTAAG 
AATCCAGTAT CAGATCACTT TCCAGTTGGA TGTATGAAAG TTGGCACTTC TTTTTCCATC 
CCGGTTGTCA GTGATGTGCG TGAGCTGGTG CCCAGCAGTG ATCCTATTGT TTTTGTGGTA 
GGGGCCTTTG CCCATGGCAA GGTCAGTGTG GAGTATACAG AGAAGATGGT GTCCATCAGT 
AACTACCCCC TTTCTGCTGC CCTCACCTGT GCAAAACTTA CCACAGCCTT TGAGGAAGTA 
TGGGGGGTCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment.
Human molecular genetics25(24)5353-5364(2016 12)
Warda AS,Freytag B,Haag S,Sloan KE,Görlich D,Bohnsack MT

The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.
Nucleic acids research38(7)2387-98(2010 Apr)
Wurm JP,Meyer B,Bahr U,Held M,Frolow O,Kötter P,Engels JW,Heckel A,Karas M,Entian KD,Wöhnert J

Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome.
American journal of human genetics84(6)728-39(2009 Jun)
Armistead J,Khatkar S,Meyer B,Mark BL,Patel N,Coghlan G,Lamont RE,Liu S,Wiechert J,Cattini PA,Koetter P,Wrogemann K,Greenberg CR,Entian KD,Zelinski T,Triggs-Raine B

A locus for Bowen-Conradi syndrome maps to chromosome region 12p13.3.
American journal of medical genetics. Part A132A(2)136-43(2005 Jan)
Lamont RE,Loredo-Osti J,Roslin NM,Mauthe J,Coghlan G,Nylen E,Frappier D,Innes AM,Lemire EG,Lowry RB,Greenberg CR,Triggs-Raine BL,Morgan K,Wrogemann K,Fujiwara TM,Zelinski T

The small-subunit processome is a ribosome assembly intermediate.
Eukaryotic cell3(6)1619-26(2004 Dec)
Bernstein KA,Gallagher JE,Mitchell BM,Granneman S,Baserga SJ

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EMG1 cDNA ORF clone, Homo sapiens(human)

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