MPHOSPH10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MPHOSPH10
Entrez Gene ID	10199
Full Name	M-phase phosphoprotein 10
Synonyms	CT90,MPP10,MPP10P,PPP1R106
General protein information	Preferred Names M-phase phosphoprotein 10 Names U3 small nucleolar ribonucleoprotein protein MPP10 U3 small nucleolar ribonucleoprotein m phase phosphoprotein 10 protein phosphatase 1, regulatory subunit 106
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.3
Summary	This gene encodes a protein that is phosphorylated during mitosis. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein associates with the U3 small nucleolar ribonucleoprotein 60-80S complexes and may be involved in pre-rRNA processing. [provided by RefSeq, Dec 2010].
Disorder MIM:	605503

mRNA and Protein(s)

mRNA	Protein	Name
NM_005791.2	NP_005782.1	U3 small nucleolar ribonucleoprotein protein MPP10
NM_005791.3	NP_005782.1	U3 small nucleolar ribonucleoprotein protein MPP10

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	MPHOSPH10	XP_001102757.1
Bos taurus (cattle)	MPHOSPH10	NP_001039782.1
Canis lupus familiaris (dog)	MPHOSPH10	XP_849351.1
Rattus norvegicus (Norway rat)	Mphosph10	NP_001099810.1
Danio rerio (zebrafish)	mphosph10	XP_709654.3
Xenopus tropicalis (tropical clawed frog)	mphosph10	NP_001186500.1
Mus musculus (house mouse)	Mphosph10	NP_080759.2
Homo sapiens (human)	MPHOSPH10	NP_005782.1
Pan troglodytes (chimpanzee)	MPHOSPH10	XP_515539.2
Gallus gallus (chicken)	MPHOSPH10	XP_413769.3

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Hendrickx A, Beullens M, Ceulemans H, Den Abt T, Van Eynde A, Nicolaescu E, Lesage B, Bollen M
Chemistry & biology16(4)365-71(2009 Apr)

Nucleolar proteome dynamics.
Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M
Nature433(7021)77-83(2005 Jan)

Functional proteomics mapping of a human signaling pathway.
Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM
Genome research14(7)1324-32(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MPHOSPH10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MPHOSPH10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28041	NM_005791.3 Latest version!	Homo sapiens M-phase phosphoprotein 10 (MPHOSPH10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu28041	NM_005791.2	Homo sapiens M-phase phosphoprotein 10 (MPHOSPH10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MPHOSPH10 ( NM_005791.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005782.1 Homo sapiens M-phase phosphoprotein 10 (MPHOSPH10), mRNA.

Price & Availability↑

CloneID	OHu28041
Clone ID Related Accession (Same CDS sequence)	NM_005791.2 , NM_005791.3
Accession Version	NM_005791.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	U3 small nucleolar ribonucleoprotein protein MPP10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA867290.1, AC007881.4, BC126389.1, BC041084.1 and AI056034.1. On Dec 17, 2010 this sequence version replaced NM_005791.1. ##Evidence-Data-START## Transcript exon combination :: AK056862.1, SRR1660807.79723.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCGC AGGTCTGGCG TCGACGGACC CTGGAGCGGT GTCTGACGGA AGTCGGCAAA 
GCCACGGGTC GGCCCGAGTG CTTCCTCACG ATTCAAGAGG GATTGGCATC AAAGTTCACT 
TCTTTAACAA AAGTGCTTTA TGACTTTAAT AAAATATTAG AGAATGGTAG GATCCATGGA 
AGCCCCTTGC AAAAACTTGT GATAGAAAAT TTTGATGATG AGCAGATTTG GCAACAACTG 
GAATTGCAAA ATGAACCAAT TTTACAATAC TTTCAGAATG CAGTTAGTGA AACAATTAAT 
GATGAAGATA TCAGTCTTCT CCCAGAGAGT GAAGAACAGG AACGTGAAGA GGATGGTTCA 
GAGATAGAGG CTGATGACAA GGAGGACCTA GAAGATTTAG AGGAGGAGGA AGTGTCCGAC 
ATGGGTAATG ATGATCCTGA AATGGGTGAG AGAGCTGAAA ACTCAAGCAA ATCTGATCTG 
AGGAAAAGCC CCGTTTTCAG TGATGAGGAT TCTGACCTTG ACTTTGATAT CAGCAAATTG 
GAACAGCAGA GCAAGGTGCA AAACAAAGGA CAGGGAAAAC CAAGAGAAAA GTCCATAGTA 
GATGATAAAT TCTTCAAACT CTCTGAAATG GAGGCCTATT TAGAAAACAT AGAAAAAGAA 
GAGGAACGAA AAGATGATAA TGATGAGGAG GAGGAAGATA TTGATTTTTT TGAAGATATT 
GATTCTGATG AAGATGAAGG GGGACTGTTT GGAAGTAAAA AACTTAAGTC AGGTAAAAGT 
TCCAGAAATC TGAAATACAA AGATTTTTTT GATCCAGTTG AAAGTGATGA AGACATAACA 
AATGTTCATG ATGATGAGCT GGATTCAAAC AAAGAAGATG ATGAAATTGC TGAAGAAGAA 
GCAGAAGAAC TAAGTATTTC GGAAACGGAT GAAGATGATG ACCTTCAAGA AAATGAAGAC 
AATAAACAAC ATAAAGAAAG CTTGAAAAGA GTGACCTTTG CTTTACCAGA TGATGCGGAA 
ACTGAAGATA CAGGTGTTTT AAATGTAAAG AAAAATTCTG ATGAAGTTAA ATCCTCCTTT 
GAAAAAAGAC AGGAAAAGAT GAATGAAAAA ATTGCATCTT TAGAAAAAGA GTTGTTAGAA 
AAAAAGCCGT GGCAGCTTCA GGGGGAAGTG ACAGCACAGA AGAGGCCAGA GAACAGCCTC 
CTGGAGGAGA CCCTACACTT TGACCATGCT GTCCGGATGG CACCTGTGAT TACAGAGGAA 
ACCACCCTTC AACTGGAAGA TATCATTAAA CAGAGGATAA GAGATCAGGC TTGGGATGAT 
GTAGTACGTA AAGAAAAACC TAAAGAGGAT GCATATGAAT ATAAAAAGCG TTTAACCTTA 
GACCATGAGA AGAGTAAATT GAGCCTTGCT GAAATTTATG AACAGGAGTA CATCAAACTC 
AACCAGCAAA AAACAGCAGA AGAAGAAAAT CCAGAACATG TAGAAATTCA GAAGATGATG 
GATTCCCTCT TCTTAAAATT GGATGCCCTC TCAAACTTCC ACTTTATCCC TAAACCGCCT 
GTACCAGAGA TTAAAGTTGT GTCAAATCTG CCAGCCATAA CCATGGAGGA AGTAGCCCCA 
GTGAGTGTTA GTGATGCAGC TCTCCTGGCC CCAGAGGAGA TCAAGGAGAA AAATAAAGCT 
GGAGATATAA AAACAGCTGC TGAAAAAACA GCTACAGACA AGAAACGAGA GCGAAGGAAA 
AAGAAATATC AAAAGCGTAT GAAAATAAAA GAGAAGGAGA AGCGGAGAAA ACTGCTTGAA 
AAGAGCAGTG TAGATCAAGC AGGGAAATAC AGCAAAACAG TAGCTTCGGA GAAGTTAAAA 
CAGCTGACCA AAACTGGCAA AGCTTCCTTC ATAAAGGATG AAGGTAAAGA CAAGGCCTTA 
AAGTCCTCTC AAGCATTCTT TTCTAAATTA CAAGATCAAG TAAAAATGCA AATCAATGAT 
GCAAAGAAAA CAGAAAAGAA AAAGAAGAAA AGACAGGATA TTTCTGTTCA TAAATTAAAG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005782.1
CDS	353..2398
Translation	MAPQVWRRRTLERCLTEVGKATGRPECFLTIQEGLASKFTSLTKVLYDFNKILENGRIHGSPLQKLVIENFDDEQIWQQLELQNEPILQYFQNAVSETINDEDISLLPESEEQEREEDGSEIEADDKEDLEDLEEEEVSDMGNDDPEMGERAENSSKSDLRKSPVFSDEDSDLDFDISKLEQQSKVQNKGQGKPREKSIVDDKFFKLSEMEAYLENIEKEEERKDDNDEEEEDIDFFEDIDSDEDEGGLFGSKKLKSGKSSRNLKYKDFFDPVESDEDITNVHDDELDSNKEDDEIAEEEAEELSISETDEDDDLQENEDNKQHKESLKRVTFALPDDAETEDTGVLNVKKNSDEVKSSFEKRQEKMNEKIASLEKELLEKKPWQLQGEVTAQKRPENSLLEETLHFDHAVRMAPVITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIYEQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVSNLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATDKKRERRKKKYQKRMKIKEKEKRRKLLEKSSVDQAGKYSKTVASEKLKQLTKTGKASFIKDEGKDKALKSSQAFFSKLQDQVKMQINDAKKTEKKKKKRQDISVHKLKL

Target ORF information:

RefSeq Version	NM_005791.2
Organism	Homo sapiens(human)
Definition	Homo sapiens M-phase phosphoprotein 10 (MPHOSPH10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005791.2

ORF Insert Sequence:

ATGGCGCCGC AGGTCTGGCG TCGACGGACC CTGGAGCGGT GTCTGACGGA AGTCGGCAAA 
GCCACGGGTC GGCCCGAGTG CTTCCTCACG ATTCAAGAGG GATTGGCATC AAAGTTCACT 
TCTTTAACAA AAGTGCTTTA TGACTTTAAT AAAATATTAG AGAATGGTAG GATCCATGGA 
AGCCCCTTGC AAAAACTTGT GATAGAAAAT TTTGATGATG AGCAGATTTG GCAACAACTG 
GAATTGCAAA ATGAACCAAT TTTACAATAC TTTCAGAATG CAGTTAGTGA AACAATTAAT 
GATGAAGATA TCAGTCTTCT CCCAGAGAGT GAAGAACAGG AACGTGAAGA GGATGGTTCA 
GAGATAGAGG CTGATGACAA GGAGGACCTA GAAGATTTAG AGGAGGAGGA AGTGTCCGAC 
ATGGGTAATG ATGATCCTGA AATGGGTGAG AGAGCTGAAA ACTCAAGCAA ATCTGATCTG 
AGGAAAAGCC CCGTTTTCAG TGATGAGGAT TCTGACCTTG ACTTTGATAT CAGCAAATTG 
GAACAGCAGA GCAAGGTGCA AAACAAAGGA CAGGGAAAAC CAAGAGAAAA GTCCATAGTA 
GATGATAAAT TCTTCAAACT CTCTGAAATG GAGGCCTATT TAGAAAACAT AGAAAAAGAA 
GAGGAACGAA AAGATGATAA TGATGAGGAG GAGGAAGATA TTGATTTTTT TGAAGATATT 
GATTCTGATG AAGATGAAGG GGGACTGTTT GGAAGTAAAA AACTTAAGTC AGGTAAAAGT 
TCCAGAAATC TGAAATACAA AGATTTTTTT GATCCAGTTG AAAGTGATGA AGACATAACA 
AATGTTCATG ATGATGAGCT GGATTCAAAC AAAGAAGATG ATGAAATTGC TGAAGAAGAA 
GCAGAAGAAC TAAGTATTTC GGAAACGGAT GAAGATGATG ACCTTCAAGA AAATGAAGAC 
AATAAACAAC ATAAAGAAAG CTTGAAAAGA GTGACCTTTG CTTTACCAGA TGATGCGGAA 
ACTGAAGATA CAGGTGTTTT AAATGTAAAG AAAAATTCTG ATGAAGTTAA ATCCTCCTTT 
GAAAAAAGAC AGGAAAAGAT GAATGAAAAA ATTGCATCTT TAGAAAAAGA GTTGTTAGAA 
AAAAAGCCGT GGCAGCTTCA GGGGGAAGTG ACAGCACAGA AGAGGCCAGA GAACAGCCTC 
CTGGAGGAGA CCCTACACTT TGACCATGCT GTCCGGATGG CACCTGTGAT TACAGAGGAA 
ACCACCCTTC AACTGGAAGA TATCATTAAA CAGAGGATAA GAGATCAGGC TTGGGATGAT 
GTAGTACGTA AAGAAAAACC TAAAGAGGAT GCATATGAAT ATAAAAAGCG TTTAACCTTA 
GACCATGAGA AGAGTAAATT GAGCCTTGCT GAAATTTATG AACAGGAGTA CATCAAACTC 
AACCAGCAAA AAACAGCAGA AGAAGAAAAT CCAGAACATG TAGAAATTCA GAAGATGATG 
GATTCCCTCT TCTTAAAATT GGATGCCCTC TCAAACTTCC ACTTTATCCC TAAACCGCCT 
GTACCAGAGA TTAAAGTTGT GTCAAATCTG CCAGCCATAA CCATGGAGGA AGTAGCCCCA 
GTGAGTGTTA GTGATGCAGC TCTCCTGGCC CCAGAGGAGA TCAAGGAGAA AAATAAAGCT 
GGAGATATAA AAACAGCTGC TGAAAAAACA GCTACAGACA AGAAACGAGA GCGAAGGAAA 
AAGAAATATC AAAAGCGTAT GAAAATAAAA GAGAAGGAGA AGCGGAGAAA ACTGCTTGAA 
AAGAGCAGTG TAGATCAAGC AGGGAAATAC AGCAAAACAG TAGCTTCGGA GAAGTTAAAA 
CAGCTGACCA AAACTGGCAA AGCTTCCTTC ATAAAGGATG AAGGTAAAGA CAAGGCCTTA 
AAGTCCTCTC AAGCATTCTT TTCTAAATTA CAAGATCAAG TAAAAATGCA AATCAATGAT 
GCAAAGAAAA CAGAAAAGAA AAAGAAGAAA AGACAGGATA TTTCTGTTCA TAAATTAAAG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MPHOSPH10 ( NM_005791.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005782.1 Homo sapiens M-phase phosphoprotein 10 (MPHOSPH10), mRNA.

Price & Availability↑

CloneID	OHu28041
Clone ID Related Accession (Same CDS sequence)	NM_005791.2 , NM_005791.3
Accession Version	NM_005791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	U3 small nucleolar ribonucleoprotein protein MPP10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC126389.1 and BC041084.1. On Nov 23, 2018 this sequence version replaced NM_005791.2. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.151375.1, SRR1660803.210472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000244230.7/ ENSP00000244230.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCCGC AGGTCTGGCG TCGACGGACC CTGGAGCGGT GTCTGACGGA AGTCGGCAAA 
GCCACGGGTC GGCCCGAGTG CTTCCTCACG ATTCAAGAGG GATTGGCATC AAAGTTCACT 
TCTTTAACAA AAGTGCTTTA TGACTTTAAT AAAATATTAG AGAATGGTAG GATCCATGGA 
AGCCCCTTGC AAAAACTTGT GATAGAAAAT TTTGATGATG AGCAGATTTG GCAACAACTG 
GAATTGCAAA ATGAACCAAT TTTACAATAC TTTCAGAATG CAGTTAGTGA AACAATTAAT 
GATGAAGATA TCAGTCTTCT CCCAGAGAGT GAAGAACAGG AACGTGAAGA GGATGGTTCA 
GAGATAGAGG CTGATGACAA GGAGGACCTA GAAGATTTAG AGGAGGAGGA AGTGTCCGAC 
ATGGGTAATG ATGATCCTGA AATGGGTGAG AGAGCTGAAA ACTCAAGCAA ATCTGATCTG 
AGGAAAAGCC CCGTTTTCAG TGATGAGGAT TCTGACCTTG ACTTTGATAT CAGCAAATTG 
GAACAGCAGA GCAAGGTGCA AAACAAAGGA CAGGGAAAAC CAAGAGAAAA GTCCATAGTA 
GATGATAAAT TCTTCAAACT CTCTGAAATG GAGGCCTATT TAGAAAACAT AGAAAAAGAA 
GAGGAACGAA AAGATGATAA TGATGAGGAG GAGGAAGATA TTGATTTTTT TGAAGATATT 
GATTCTGATG AAGATGAAGG GGGACTGTTT GGAAGTAAAA AACTTAAGTC AGGTAAAAGT 
TCCAGAAATC TGAAATACAA AGATTTTTTT GATCCAGTTG AAAGTGATGA AGACATAACA 
AATGTTCATG ATGATGAGCT GGATTCAAAC AAAGAAGATG ATGAAATTGC TGAAGAAGAA 
GCAGAAGAAC TAAGTATTTC GGAAACGGAT GAAGATGATG ACCTTCAAGA AAATGAAGAC 
AATAAACAAC ATAAAGAAAG CTTGAAAAGA GTGACCTTTG CTTTACCAGA TGATGCGGAA 
ACTGAAGATA CAGGTGTTTT AAATGTAAAG AAAAATTCTG ATGAAGTTAA ATCCTCCTTT 
GAAAAAAGAC AGGAAAAGAT GAATGAAAAA ATTGCATCTT TAGAAAAAGA GTTGTTAGAA 
AAAAAGCCGT GGCAGCTTCA GGGGGAAGTG ACAGCACAGA AGAGGCCAGA GAACAGCCTC 
CTGGAGGAGA CCCTACACTT TGACCATGCT GTCCGGATGG CACCTGTGAT TACAGAGGAA 
ACCACCCTTC AACTGGAAGA TATCATTAAA CAGAGGATAA GAGATCAGGC TTGGGATGAT 
GTAGTACGTA AAGAAAAACC TAAAGAGGAT GCATATGAAT ATAAAAAGCG TTTAACCTTA 
GACCATGAGA AGAGTAAATT GAGCCTTGCT GAAATTTATG AACAGGAGTA CATCAAACTC 
AACCAGCAAA AAACAGCAGA AGAAGAAAAT CCAGAACATG TAGAAATTCA GAAGATGATG 
GATTCCCTCT TCTTAAAATT GGATGCCCTC TCAAACTTCC ACTTTATCCC TAAACCGCCT 
GTACCAGAGA TTAAAGTTGT GTCAAATCTG CCAGCCATAA CCATGGAGGA AGTAGCCCCA 
GTGAGTGTTA GTGATGCAGC TCTCCTGGCC CCAGAGGAGA TCAAGGAGAA AAATAAAGCT 
GGAGATATAA AAACAGCTGC TGAAAAAACA GCTACAGACA AGAAACGAGA GCGAAGGAAA 
AAGAAATATC AAAAGCGTAT GAAAATAAAA GAGAAGGAGA AGCGGAGAAA ACTGCTTGAA 
AAGAGCAGTG TAGATCAAGC AGGGAAATAC AGCAAAACAG TAGCTTCGGA GAAGTTAAAA 
CAGCTGACCA AAACTGGCAA AGCTTCCTTC ATAAAGGATG AAGGTAAAGA CAAGGCCTTA 
AAGTCCTCTC AAGCATTCTT TTCTAAATTA CAAGATCAAG TAAAAATGCA AATCAATGAT 
GCAAAGAAAA CAGAAAAGAA AAAGAAGAAA AGACAGGATA TTTCTGTTCA TAAATTAAAG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005782.1
CDS	33..2078
Translation	MAPQVWRRRTLERCLTEVGKATGRPECFLTIQEGLASKFTSLTKVLYDFNKILENGRIHGSPLQKLVIENFDDEQIWQQLELQNEPILQYFQNAVSETINDEDISLLPESEEQEREEDGSEIEADDKEDLEDLEEEEVSDMGNDDPEMGERAENSSKSDLRKSPVFSDEDSDLDFDISKLEQQSKVQNKGQGKPREKSIVDDKFFKLSEMEAYLENIEKEEERKDDNDEEEEDIDFFEDIDSDEDEGGLFGSKKLKSGKSSRNLKYKDFFDPVESDEDITNVHDDELDSNKEDDEIAEEEAEELSISETDEDDDLQENEDNKQHKESLKRVTFALPDDAETEDTGVLNVKKNSDEVKSSFEKRQEKMNEKIASLEKELLEKKPWQLQGEVTAQKRPENSLLEETLHFDHAVRMAPVITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIYEQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVSNLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATDKKRERRKKKYQKRMKIKEKEKRRKLLEKSSVDQAGKYSKTVASEKLKQLTKTGKASFIKDEGKDKALKSSQAFFSKLQDQVKMQINDAKKTEKKKKKRQDISVHKLKL

Target ORF information:

RefSeq Version	NM_005791.3
Organism	Homo sapiens(human)
Definition	Homo sapiens M-phase phosphoprotein 10 (MPHOSPH10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005791.3

ORF Insert Sequence:

ATGGCGCCGC AGGTCTGGCG TCGACGGACC CTGGAGCGGT GTCTGACGGA AGTCGGCAAA 
GCCACGGGTC GGCCCGAGTG CTTCCTCACG ATTCAAGAGG GATTGGCATC AAAGTTCACT 
TCTTTAACAA AAGTGCTTTA TGACTTTAAT AAAATATTAG AGAATGGTAG GATCCATGGA 
AGCCCCTTGC AAAAACTTGT GATAGAAAAT TTTGATGATG AGCAGATTTG GCAACAACTG 
GAATTGCAAA ATGAACCAAT TTTACAATAC TTTCAGAATG CAGTTAGTGA AACAATTAAT 
GATGAAGATA TCAGTCTTCT CCCAGAGAGT GAAGAACAGG AACGTGAAGA GGATGGTTCA 
GAGATAGAGG CTGATGACAA GGAGGACCTA GAAGATTTAG AGGAGGAGGA AGTGTCCGAC 
ATGGGTAATG ATGATCCTGA AATGGGTGAG AGAGCTGAAA ACTCAAGCAA ATCTGATCTG 
AGGAAAAGCC CCGTTTTCAG TGATGAGGAT TCTGACCTTG ACTTTGATAT CAGCAAATTG 
GAACAGCAGA GCAAGGTGCA AAACAAAGGA CAGGGAAAAC CAAGAGAAAA GTCCATAGTA 
GATGATAAAT TCTTCAAACT CTCTGAAATG GAGGCCTATT TAGAAAACAT AGAAAAAGAA 
GAGGAACGAA AAGATGATAA TGATGAGGAG GAGGAAGATA TTGATTTTTT TGAAGATATT 
GATTCTGATG AAGATGAAGG GGGACTGTTT GGAAGTAAAA AACTTAAGTC AGGTAAAAGT 
TCCAGAAATC TGAAATACAA AGATTTTTTT GATCCAGTTG AAAGTGATGA AGACATAACA 
AATGTTCATG ATGATGAGCT GGATTCAAAC AAAGAAGATG ATGAAATTGC TGAAGAAGAA 
GCAGAAGAAC TAAGTATTTC GGAAACGGAT GAAGATGATG ACCTTCAAGA AAATGAAGAC 
AATAAACAAC ATAAAGAAAG CTTGAAAAGA GTGACCTTTG CTTTACCAGA TGATGCGGAA 
ACTGAAGATA CAGGTGTTTT AAATGTAAAG AAAAATTCTG ATGAAGTTAA ATCCTCCTTT 
GAAAAAAGAC AGGAAAAGAT GAATGAAAAA ATTGCATCTT TAGAAAAAGA GTTGTTAGAA 
AAAAAGCCGT GGCAGCTTCA GGGGGAAGTG ACAGCACAGA AGAGGCCAGA GAACAGCCTC 
CTGGAGGAGA CCCTACACTT TGACCATGCT GTCCGGATGG CACCTGTGAT TACAGAGGAA 
ACCACCCTTC AACTGGAAGA TATCATTAAA CAGAGGATAA GAGATCAGGC TTGGGATGAT 
GTAGTACGTA AAGAAAAACC TAAAGAGGAT GCATATGAAT ATAAAAAGCG TTTAACCTTA 
GACCATGAGA AGAGTAAATT GAGCCTTGCT GAAATTTATG AACAGGAGTA CATCAAACTC 
AACCAGCAAA AAACAGCAGA AGAAGAAAAT CCAGAACATG TAGAAATTCA GAAGATGATG 
GATTCCCTCT TCTTAAAATT GGATGCCCTC TCAAACTTCC ACTTTATCCC TAAACCGCCT 
GTACCAGAGA TTAAAGTTGT GTCAAATCTG CCAGCCATAA CCATGGAGGA AGTAGCCCCA 
GTGAGTGTTA GTGATGCAGC TCTCCTGGCC CCAGAGGAGA TCAAGGAGAA AAATAAAGCT 
GGAGATATAA AAACAGCTGC TGAAAAAACA GCTACAGACA AGAAACGAGA GCGAAGGAAA 
AAGAAATATC AAAAGCGTAT GAAAATAAAA GAGAAGGAGA AGCGGAGAAA ACTGCTTGAA 
AAGAGCAGTG TAGATCAAGC AGGGAAATAC AGCAAAACAG TAGCTTCGGA GAAGTTAAAA 
CAGCTGACCA AAACTGGCAA AGCTTCCTTC ATAAAGGATG AAGGTAAAGA CAAGGCCTTA 
AAGTCCTCTC AAGCATTCTT TTCTAAATTA CAAGATCAAG TAAAAATGCA AATCAATGAT 
GCAAAGAAAA CAGAAAAGAA AAAGAAGAAA AGACAGGATA TTTCTGTTCA TAAATTAAAG 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Docking motif-guided mapping of the interactome of protein phosphatase-1.
Chemistry & biology16(4)365-71(2009 Apr)
Hendrickx A,Beullens M,Ceulemans H,Den Abt T,Van Eynde A,Nicolaescu E,Lesage B,Bollen M

Nucleolar proteome dynamics.
Nature433(7021)77-83(2005 Jan)
Andersen JS,Lam YW,Leung AK,Ong SE,Lyon CE,Lamond AI,Mann M

Functional proteomics mapping of a human signaling pathway.
Genome research14(7)1324-32(2004 Jul)
Colland F,Jacq X,Trouplin V,Mougin C,Groizeleau C,Hamburger A,Meil A,Wojcik J,Legrain P,Gauthier JM

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