NUMA1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUMA1
Entrez Gene ID	768717
Full Name	nuclear mitotic apparatus protein 1
Synonyms	NUMA
General protein information	Preferred Names nuclear mitotic apparatus protein 1 Names nuclear mitotic apparatus protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015280865.2	XP_015136351.1	nuclear mitotic apparatus protein 1 isoform X1
NM_001190925.1	NP_001177854.1	nuclear mitotic apparatus protein 1
XM_015280865.1	XP_015136351.1	nuclear mitotic apparatus protein 1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NUMA1	NP_001177854.1
Pan troglodytes (chimpanzee)	NUMA1	XP_001174474.1
Macaca mulatta (Rhesus monkey)	NUMA1	XP_001114111.2
Rattus norvegicus (Norway rat)	Numa1	NP_001094161.1
Bos taurus (cattle)	NUMA1	NP_001192675.1
Homo sapiens (human)	NUMA1	NP_006176.2
Canis lupus familiaris (dog)	NUMA1	XP_005633612.1
Xenopus tropicalis (tropical clawed frog)	LOC100145664	XP_004920191.1
Mus musculus (house mouse)	Numa1	NP_598708.3

Gene Ontology
Bibliography

Related articles in PubMed

A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells.
Peyre E, Jaouen F, Saadaoui M, Haren L, Merdes A, Durbec P, Morin X
The Journal of cell biology193(1)141-54(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NUMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa25637	NM_001190925.1 Latest version!	Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa31166	XM_015280865.2 Latest version!	Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OGa31166	XM_015280865.1	Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUMA1 ( NM_001190925.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001177854.1 Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), mRNA.

Price & Availability↑

CloneID	OGa25637
Clone ID Related Accession (Same CDS sequence)	NM_001190925.1
Accession Version	NM_001190925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526745600000
Organism	Gallus gallus(chicken)
Product	nuclear mitotic apparatus protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FN994986.1. On Jul 6, 2010 this sequence version replaced XM_001231804.1. ##Evidence-Data-START## Transcript exon combination :: FN994986.1 [ECO:0000332] ##Evidence-Data-END##

ATGCCGCTCC ACACCACCAG AGCCACCGCG CTCCTCGCTT GGGTGAACAG CACCAAGGCG 
TGCGCGGAGC CACTTGGCGA CCTGTCCCAG CTGCAGGACT GCCGTGTTTT CATCCAGATC 
ATCAATAAGA TCCACAGGAC TGAGGAGGGG GAGTCCGTCC TGGAGCAGTC CCTGGCGGAG 
CGAGCAGCCT TCATCTGTGG CTTCCTGCAG AAGCTCTGCA AGCACAAGTC GGCCACGGAG 
AACCTGGTGT CAGCACAGAA GCTGCTGGAA GGAGAAGAGC TGGAGTTGGC CAAGGTAGCC 
GTGCTGCTCC TGTACCACAG CTCCATGAGC AGCAAGAGCC CGCGGGACTG GAATGAGTTC 
GATTACCAGA TCCAGGTCGA GCTGGCCACC ATCCTCAAGT TCGTGCTGGA CCATGAGGAG 
AGCTTGAGTG AGAACCTGGA GGTGTTTCTG CAGCGCAGAG CGCCGCTATC CTCGCCCGGC 
ACATCCAGCA GCAGCTCTGA GGAGCGCTCG CCGGGGCTCT CCCACCCTCA GGTGCGCTTC 
CTGGAGCTGC AGAAGATCGC CTCCTCCTCC TCCAGCATGA ACAACATGCT CCCTGGAACA 
CCAGCCTCAC CTGTGGGGGA TGTCCTGCAG ACCCCCCAGT TCCAGCTGCG GAGGTTGAAG 
AAGCAGCTGG CTATTGAGAG GGAGAACCGG GACGAGCTGG AGATGGAGCT GGCAGAGAAC 
CGCAAACTCA TCACCGAGAA GGAGGCTCAG ATCACCATGA TGCAGCACCG CATCGACCGC 
TTGGCTCTGC TCAATGAGAA GCAAGCTGCA GACCAGCTGG AGCCCAGAGA GATGGAGGAG 
CTGAGGGAGA GGAACGAGAG CCTGCTGGTG CGCTTGCACG AGGCCCTGAA GCAGTGCCAG 
GATCTGAAGA CCGAGAAGGG CCAGATGGAC CGCAAGATCA ACCAGCTCTC CGAGGAGAAC 
GGGGACCTCT CCTTCAAGCT GAGGGAGATC GCCAGCCACC TGGTGCAGCT GCAGGATGCC 
CTGAATGAGC TCTCCGAGGA GCACAACTCT GCGCTGATGC AGTCCCAAGC AAAGCAGGCG 
CAGCTGGAGG GCGAGCTGCG GGCTGTGCTG CAGGAGAAGA AATGCCTAGA AGAGAAGGTT 
GAGATCCTTC AGGGGAAGAT TTCCCTGCTG GAAGACCAGC TGGTCAAGCT GGGGGACTGC 
AGCACGCAGG AAAAGGGAGA GGTCATGGGC GACGTCCTGA AGCTGGAAGA GCTCAAGCAG 
GAGGTGTCCA GCCTCACCAC CAAAGGCATT GAGCTCCAAG CCTCCGTCCT GCGGCTGGAG 
GAAGAGAAGA GCCAGCGGGA TGCAGCCCTG CAGGCGGAGC GCAACCACTT CGAGATGGAG 
AAGCTCCAGC TTGGCACCCA CATCTCCAAC CTGCAGAGCT CCGTCTCCGA GCTGCACCTG 
GCCAAGGAGA AGCTGGAGCA GGAGCTGCGG GCGCAGGAGG CGCACCTGAC GGCCCAGGCA 
GACGCACTGA CAGCAGAGGT GGGCAAGCTG AGTGGATTCC TGCAGCAGCA GGAGAAGGAG 
GCAGCTGAGC TGCGGATGCA GGCAGAGCAG GAGCGGGCGC AGGCGGAGGA GCTGCGGCAC 
CGGGATATGG CCTCACAGGA GGCTCTCAGC GAGCTGAGCC GGCGTGTGGA GCAGCTGGGT 
GCCAGCCTGA AGCACAGCGA GGAGGAGCTG GTGCGGGTCA CGCAGGAGGC GGAAGGGAAA 
GCCAGACAGC TCGGGGCTGA ACACGAGAAA GCTGTCCAGG AGAGGGATGC AGCCCTGCAG 
CAGCTGCAGC ACTTGCAGCA AGCCAAGGAA AAGCAGCTGG CGGCACTGAG TAGCCAACTA 
CAGAGCCTGG AGGCGGCCAG CAAAGCTTCG TCCATGGAAA TGCAGCAGGA GAAGGCTGAG 
TTGAATCAGA AGGTCCAAGA GCTGCAAGCC CGCGTCCTTG AGCTCAGCGC ACAGTGCCAG 
CAGAGCTCAG CCCAAGCAGG GAGTGCTGAG ACGCTGCGAG CCCAGTTGCG GGAGCTGGAG 
GGCAAGCTGA AGGACAGCCA GCAGAAGCTG GCTGACAAGG AGAAGGTGGC CAAGGAGAAC 
ACGCGCCTGC AGGAGCGGCT GCTCTTCCTG GAGGAGTCAG TGCGCAACAC TGAAGGCATC 
CTGGAGGACG AGAAGAGGAG AGCAGCGGAG AGCCTGGAGG GGAATCTGGC CCGGATCGCA 
GAGTTGGAGG CGGAGAAGCA GCAGCTGGTG CAGCGTGGTG AGCAGGCCCT GCAGCAGCAC 
AGCGAGGAGC TGGCCAGGAG GCAGGCACTG GAGACACGGC TGCAGCAGGT GGCAGAGGAG 
CGCCGGGAGG AGACGGCAGC CCTGCAGCGT CAGCTGGCCG AGGTGGCCAA GGGGGAGGAA 
GGAGAGCGCG GCAGGCTGGA GAAGAGGCTG CAGGAGCTGA GCCGGGAGCA CGGGCAGGCC 
TGCCAGCGGC TGCAGGCCGA GCAGGAGAGG GTGGCAGAGC TGGAAGCCCG CGTGGCACGC 
ATGGCCAGCG AGCAGCAGGA GCAGCTGGCC GCCCTCCAGG ACCAGCTGGC CAGAGCTAAG 
GAGAAGGAAG GCAAGGAGCA GGAAAAGCTG GCGAGCGCCG AGCGCGTGGG GAAGTTGGAG 
GCGGAGGTGC GGAAGGCGAG CGAAGCGCTC GAGGCCGTCT CCAAGGAGCT GTCAGAGGAG 
AGGCTGAAAT TCAAGGAGCT CGAAGCTGTG GCCACGCAGG AGGCCAGCGA GGTGGCCCGG 
CTGAGCGCGG CGCTGGAGGA GGCGGCACGG GAGCACGGGC AGGAGCTGGC ACGGCGCGAG 
GAGGAGGCAA AGCGCCTGCA GAAGGAGCTG GAGGACGCCA AGGCCGATTG CGCCGCCGAG 
AAAGCACGCA AGGTAGAGCT GGAGGTACAG CTGCAGAACT CCATCAACGA GCAGCGGGTG 
GAGCGCTCGG CGCACCAGCA GGAACTGGCG CGGTCCCTGG AGCTGATCGA GGAGAAGGAG 
GGAGAGCTGG ATGAGCTGCG GCTGAAGAAC GTCTCGCGGG GTGAGGAGCT GAGGGACCTG 
CAGAAGACAG TCAGCAAGCT GAAAGGGGAG CTCGCCTCGG TGGAAGCAGT GAAGGAGCGG 
GCGTCCAAGA TGGACAGCGA GCTGCAGGGC TTCCTGGAGG CCGCCCGGAG CCGGGATGCT 
GAGATGGACA GCATCAAGGC AGTGTACGCC AAGGAGGCGT CCCTCAAAAA CCTGGAGGAG 
AAGATCCGCC ACCGAGAGCA GGAGTCTGGC TCCAGCCAAG ACCTCTACCA GGAGAAGCTG 
AAGGAGGCCC AGATGCTGAG CGTGGAAGTG GAGCGGCTGG AGCAGAAGTG CCGGGAGCAG 
CAGGACACCA TCGCTGGGCT GGAGAAAGCC GTGGCTGAAG GATCCCAGCA GCAACAGGCG 
GAGCTGGAGG CATTGCAGAG GGAAGCAGTG CGGCACCGGG AGACGGCAGC GGAGCTGCAG 
AGGCTGCTGG ATGCCTCGCG CTCAGCCCAG GCCCTGCAGG ATGGCACCGT GGAGAGCCTG 
AGGAAGGAGC TGCAGGACAA GAGCAAGGAG CTGGCCCAGA GCAAAATGGC TGTGGCTGCT 
GCCGAGAAGG AGGTGGCATC CCTGCGTGCT GCGGCGCAGG AGAAGGGCCA CCTGGAGGAG 
GGCTGGAAGG AGCAGCTGTC GCAATGCATC CAGGAGCTGG AGAGGAAGAA CAGCCTGCTG 
GGCAGCCTGG AGCATGAGGT GTCCATCCTG CACCGGCAGC TCACCGAGAA GGAGGGCGAG 
AGCAAGGAGC TGAAACGCCT GATCATGGCC GAGTCGGAGA AGAGCAAGAA GCTGGAGGAG 
AGGCTGCGGG TGCTGCAGAC CGAGATGGCC ACGGCCGCAT CGCGGGCGGC CGAACGCTGC 
TCGCTGATGA AGGTGGAGGT GCAACGGTGC CAGGAGGAGA TGGAGAAGCA ACGGATGACC 
ATCGAGGCAC TGAAGAGGGA CCGGCACTGC CAGAGCGAGC GCGAGGAGGA GCTGCGGCAG 
GAGGTGAAGG TCTGCCAGGA CAAGTTCTTC CAGAAGGAGC AGCTCCTCTC CTCCCTGCAG 
CAGGAGCTGG GCAGCGCCCA GGCGTTGGCC GGAGAGGTGG TGCCGCTGAA ACACCTGTGC 
CAGCAGCTGC AAGCTGAGCG TGCCTCGCTG GAGAGCAAGC ACCGCGAGGA CCTGGAGCAA 
AGGGCAAAGG CCACCACTGC GCTGCAGGCG GAGCTGGCCC GGGCCAGGGT GGAGGTGGCC 
GAGCTGCCGG CCCTGCGTGA CAGAGCAGCT GAGCAGGAGC GTGCGCTGCA GAGGCTGCAG 
AAGGAGACAG CGAGCTCCAG CGAGAGGCTG GCAGCCCTGC AAGCAGCCAA CAGCCGGCTG 
GCTGAGGAGA ACCGTGCGCT GAGTGAGAGC GTGAGCCGCG GGCAGCAGCG CCTGGATGCT 
GAGCTGGGGC AGGCAAGGGA GAAGCACACA CGGGAGCTGG AGTGCGTGAG GCTGGAGGCG 
GAGAAGGCGA TGGCCAGCAG CAGGCAGGAG GCCGAGGAGG CGGCCAGGAA GCTGGAGGCG 
ATGAGCAATA AGTATGAAAA CACCAAGGTA AAGGTGCTGG AGGAGAGGCA GAAGTTCCAG 
GAGGAGAGGC AGAAGCTGAT GGCGCAGGTG GAGCAGCTAG AGGTATTTCA GAAAGAACAA 
GCAAAGCAGG TGGAGGAGCT GAATAAGAAG CTGGTGCAGC ACGAGAAGGC CACGCGGAGC 
CAGCAGCAGA GAGTTAAGGT GCTGGAAGGA GAGCTGCAGA GCGAGGCCAC GCGCCAGCAG 
GAGAGGGTGG CAGAGCTGGA GGAGCAGCTG GCACAGAAGG AGCAGGCAGC TGAGCACTAC 
AAAGCCCAGA TGGAAAAGGC GAAAACTCAC TACGACGCAA AGAAGCAGCA GAACCAGGAC 
CTGGCAGAGA AGCTGAAGGC CATGGAGCAG CTGCAGAAGG AGAACGCGGA GCTGAGGACG 
GAGTCGGAGC GCTTGGCCAA GGAGCTGCAG CAGAGCGTGC TGCAGGCCAA GGAGGCCGAG 
CTGAGCTGCA GGGAGCTGAG CGGGCAGGTC CGCAGTCTGG AGACACAGCT GGAATTCGCC 
GACCAGCAGC TTCGCGAGCT CGGCAAGTTC CCAGCCCCCA AGGCCACGCT GAAGGAACCG 
GAGAGCTTCC GCCAGAACCC CTCCGACCTC AGCACTGACA GCCTGGACCT CAGCCTGGAC 
GAGGGGCAGC CCCTCAACTC CACCAGGAAA GCCACCCGCT CGCACTCGGA CGGCTCAGCC 
GCTCCGGGTG GCACAGAGCC ACGCGCATCC CAGCGGCTGC CAAGGAAGGT GGAGTCATTG 
GAGAGCCTCT ACTTCACACC CATCGCCAGC CGCACGCAGC CCAAGCTGGA GAGCAGCGCC 
GGTTCTCTGG CCGACGTCTC ACTGGAGTCG GGCTGCAGGA CGCGCTCGGG GCGCCGCCGC 
ACCACGCAGA TCATCAACAT CACCATGACC AAGAAAGAGA CCACCACGGA GGAGCCGGGC 
GGCGCGGACG CGTCCTTCAG CAGCATCCTC TCTGAGCAGC CCCAGAAAGC CGCCCCGGCG 
CGGGCCCGGC TGCGCTCTGC TCGCTCCTTG GCCAGCTTTC CCTCGCAGGA CTCCCTGAGC 
AAGCTGGACA CCTCCTCCCC TCAGGAGCCA TCAGGCCACG CTGCGCTGCT CAGCCTCCCC 
GGCTACCGGC CGGCCACGCG CAGCTCGCTG CGGCGCTCGC AGGCGGGCAG CAGCTCCAGC 
TTAGGTCGCA GCAGCATCTA CCTGGGGACG TGCCAGGACG AACCGGAGCA GCTGGACGAT 
TGGAACCGCA TCGCCGAGCT GCAGCAGCGC AACCGCGCCT GCCCGCCGCA CCTCAAGACC 
TGCTACCCCC TGGAGAGCCG GCCTTCCGAC GTGCTGGGCA CCATCACGGA CGAGGAGATG 
AAGACGGGCG ACCCGACGGA GACGCTGCGG CGCGCCAGCA TGCAGCCCTT GCAGATTGCC 
GAGGGGGCGG CCACCCGGCG CGGCACGCTG GGAGGGGGGT GGGCCCACGG GGGCATCACC 
ACCCGGCAGC AGCGCAAACG GCTCTCGGAT GAATCCCACC AAGGCCCCGA CACCCCCGAG 
TCCAAGAAGC CTGTCAGCTG CTTCCCACGC CCCCAGACCC CCCGCGAGCG GCGCAGCTCC 
CACCTCAGCC GGCGGAGCGA GCAGCAGGCG CCCAGCAAGC AGCCCGAACG GCGCCAGTCC 
ATGGCCTTCA GCATCCTCAA CACCCCCAAG AAGCTGGGGA ACAGCCTCCT GCGCCGGGCT 
GCCAACAGGA AAACCACCCC CAAGAACTCC CCCCGTGGCA CCGCGCGCCG CTCGCCCCGC 
ATCGCCTCCA CCAAATCCCC CAAGGGCAAG GCCGGCCGCA GAGCGCTGAA GGACACCAAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177854.1
CDS	43..6468
Translation	MPLHTTRATALLAWVNSTKACAEPLGDLSQLQDCRVFIQIINKIHRTEEGESVLEQSLAERAAFICGFLQKLCKHKSATENLVSAQKLLEGEELELAKVAVLLLYHSSMSSKSPRDWNEFDYQIQVELATILKFVLDHEESLSENLEVFLQRRAPLSSPGTSSSSSEERSPGLSHPQVRFLELQKIASSSSSMNNMLPGTPASPVGDVLQTPQFQLRRLKKQLAIERENRDELEMELAENRKLITEKEAQITMMQHRIDRLALLNEKQAADQLEPREMEELRERNESLLVRLHEALKQCQDLKTEKGQMDRKINQLSEENGDLSFKLREIASHLVQLQDALNELSEEHNSALMQSQAKQAQLEGELRAVLQEKKCLEEKVEILQGKISLLEDQLVKLGDCSTQEKGEVMGDVLKLEELKQEVSSLTTKGIELQASVLRLEEEKSQRDAALQAERNHFEMEKLQLGTHISNLQSSVSELHLAKEKLEQELRAQEAHLTAQADALTAEVGKLSGFLQQQEKEAAELRMQAEQERAQAEELRHRDMASQEALSELSRRVEQLGASLKHSEEELVRVTQEAEGKARQLGAEHEKAVQERDAALQQLQHLQQAKEKQLAALSSQLQSLEAASKASSMEMQQEKAELNQKVQELQARVLELSAQCQQSSAQAGSAETLRAQLRELEGKLKDSQQKLADKEKVAKENTRLQERLLFLEESVRNTEGILEDEKRRAAESLEGNLARIAELEAEKQQLVQRGEQALQQHSEELARRQALETRLQQVAEERREETAALQRQLAEVAKGEEGERGRLEKRLQELSREHGQACQRLQAEQERVAELEARVARMASEQQEQLAALQDQLARAKEKEGKEQEKLASAERVGKLEAEVRKASEALEAVSKELSEERLKFKELEAVATQEASEVARLSAALEEAAREHGQELARREEEAKRLQKELEDAKADCAAEKARKVELEVQLQNSINEQRVERSAHQQELARSLELIEEKEGELDELRLKNVSRGEELRDLQKTVSKLKGELASVEAVKERASKMDSELQGFLEAARSRDAEMDSIKAVYAKEASLKNLEEKIRHREQESGSSQDLYQEKLKEAQMLSVEVERLEQKCREQQDTIAGLEKAVAEGSQQQQAELEALQREAVRHRETAAELQRLLDASRSAQALQDGTVESLRKELQDKSKELAQSKMAVAAAEKEVASLRAAAQEKGHLEEGWKEQLSQCIQELERKNSLLGSLEHEVSILHRQLTEKEGESKELKRLIMAESEKSKKLEERLRVLQTEMATAASRAAERCSLMKVEVQRCQEEMEKQRMTIEALKRDRHCQSEREEELRQEVKVCQDKFFQKEQLLSSLQQELGSAQALAGEVVPLKHLCQQLQAERASLESKHREDLEQRAKATTALQAELARARVEVAELPALRDRAAEQERALQRLQKETASSSERLAALQAANSRLAEENRALSESVSRGQQRLDAELGQAREKHTRELECVRLEAEKAMASSRQEAEEAARKLEAMSNKYENTKVKVLEERQKFQEERQKLMAQVEQLEVFQKEQAKQVEELNKKLVQHEKATRSQQQRVKVLEGELQSEATRQQERVAELEEQLAQKEQAAEHYKAQMEKAKTHYDAKKQQNQDLAEKLKAMEQLQKENAELRTESERLAKELQQSVLQAKEAELSCRELSGQVRSLETQLEFADQQLRELGKFPAPKATLKEPESFRQNPSDLSTDSLDLSLDEGQPLNSTRKATRSHSDGSAAPGGTEPRASQRLPRKVESLESLYFTPIASRTQPKLESSAGSLADVSLESGCRTRSGRRRTTQIINITMTKKETTTEEPGGADASFSSILSEQPQKAAPARARLRSARSLASFPSQDSLSKLDTSSPQEPSGHAALLSLPGYRPATRSSLRRSQAGSSSSLGRSSIYLGTCQDEPEQLDDWNRIAELQQRNRACPPHLKTCYPLESRPSDVLGTITDEEMKTGDPTETLRRASMQPLQIAEGAATRRGTLGGGWAHGGITTRQQRKRLSDESHQGPDTPESKKPVSCFPRPQTPRERRSSHLSRRSEQQAPSKQPERRQSMAFSILNTPKKLGNSLLRRAANRKTTPKNSPRGTARRSPRIASTKSPKGKAGRRALKDTKF

Target ORF information:

RefSeq Version	NM_001190925.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190925.1

ORF Insert Sequence:

ATGCCGCTCC ACACCACCAG AGCCACCGCG CTCCTCGCTT GGGTGAACAG CACCAAGGCG 
TGCGCGGAGC CACTTGGCGA CCTGTCCCAG CTGCAGGACT GCCGTGTTTT CATCCAGATC 
ATCAATAAGA TCCACAGGAC TGAGGAGGGG GAGTCCGTCC TGGAGCAGTC CCTGGCGGAG 
CGAGCAGCCT TCATCTGTGG CTTCCTGCAG AAGCTCTGCA AGCACAAGTC GGCCACGGAG 
AACCTGGTGT CAGCACAGAA GCTGCTGGAA GGAGAAGAGC TGGAGTTGGC CAAGGTAGCC 
GTGCTGCTCC TGTACCACAG CTCCATGAGC AGCAAGAGCC CGCGGGACTG GAATGAGTTC 
GATTACCAGA TCCAGGTCGA GCTGGCCACC ATCCTCAAGT TCGTGCTGGA CCATGAGGAG 
AGCTTGAGTG AGAACCTGGA GGTGTTTCTG CAGCGCAGAG CGCCGCTATC CTCGCCCGGC 
ACATCCAGCA GCAGCTCTGA GGAGCGCTCG CCGGGGCTCT CCCACCCTCA GGTGCGCTTC 
CTGGAGCTGC AGAAGATCGC CTCCTCCTCC TCCAGCATGA ACAACATGCT CCCTGGAACA 
CCAGCCTCAC CTGTGGGGGA TGTCCTGCAG ACCCCCCAGT TCCAGCTGCG GAGGTTGAAG 
AAGCAGCTGG CTATTGAGAG GGAGAACCGG GACGAGCTGG AGATGGAGCT GGCAGAGAAC 
CGCAAACTCA TCACCGAGAA GGAGGCTCAG ATCACCATGA TGCAGCACCG CATCGACCGC 
TTGGCTCTGC TCAATGAGAA GCAAGCTGCA GACCAGCTGG AGCCCAGAGA GATGGAGGAG 
CTGAGGGAGA GGAACGAGAG CCTGCTGGTG CGCTTGCACG AGGCCCTGAA GCAGTGCCAG 
GATCTGAAGA CCGAGAAGGG CCAGATGGAC CGCAAGATCA ACCAGCTCTC CGAGGAGAAC 
GGGGACCTCT CCTTCAAGCT GAGGGAGATC GCCAGCCACC TGGTGCAGCT GCAGGATGCC 
CTGAATGAGC TCTCCGAGGA GCACAACTCT GCGCTGATGC AGTCCCAAGC AAAGCAGGCG 
CAGCTGGAGG GCGAGCTGCG GGCTGTGCTG CAGGAGAAGA AATGCCTAGA AGAGAAGGTT 
GAGATCCTTC AGGGGAAGAT TTCCCTGCTG GAAGACCAGC TGGTCAAGCT GGGGGACTGC 
AGCACGCAGG AAAAGGGAGA GGTCATGGGC GACGTCCTGA AGCTGGAAGA GCTCAAGCAG 
GAGGTGTCCA GCCTCACCAC CAAAGGCATT GAGCTCCAAG CCTCCGTCCT GCGGCTGGAG 
GAAGAGAAGA GCCAGCGGGA TGCAGCCCTG CAGGCGGAGC GCAACCACTT CGAGATGGAG 
AAGCTCCAGC TTGGCACCCA CATCTCCAAC CTGCAGAGCT CCGTCTCCGA GCTGCACCTG 
GCCAAGGAGA AGCTGGAGCA GGAGCTGCGG GCGCAGGAGG CGCACCTGAC GGCCCAGGCA 
GACGCACTGA CAGCAGAGGT GGGCAAGCTG AGTGGATTCC TGCAGCAGCA GGAGAAGGAG 
GCAGCTGAGC TGCGGATGCA GGCAGAGCAG GAGCGGGCGC AGGCGGAGGA GCTGCGGCAC 
CGGGATATGG CCTCACAGGA GGCTCTCAGC GAGCTGAGCC GGCGTGTGGA GCAGCTGGGT 
GCCAGCCTGA AGCACAGCGA GGAGGAGCTG GTGCGGGTCA CGCAGGAGGC GGAAGGGAAA 
GCCAGACAGC TCGGGGCTGA ACACGAGAAA GCTGTCCAGG AGAGGGATGC AGCCCTGCAG 
CAGCTGCAGC ACTTGCAGCA AGCCAAGGAA AAGCAGCTGG CGGCACTGAG TAGCCAACTA 
CAGAGCCTGG AGGCGGCCAG CAAAGCTTCG TCCATGGAAA TGCAGCAGGA GAAGGCTGAG 
TTGAATCAGA AGGTCCAAGA GCTGCAAGCC CGCGTCCTTG AGCTCAGCGC ACAGTGCCAG 
CAGAGCTCAG CCCAAGCAGG GAGTGCTGAG ACGCTGCGAG CCCAGTTGCG GGAGCTGGAG 
GGCAAGCTGA AGGACAGCCA GCAGAAGCTG GCTGACAAGG AGAAGGTGGC CAAGGAGAAC 
ACGCGCCTGC AGGAGCGGCT GCTCTTCCTG GAGGAGTCAG TGCGCAACAC TGAAGGCATC 
CTGGAGGACG AGAAGAGGAG AGCAGCGGAG AGCCTGGAGG GGAATCTGGC CCGGATCGCA 
GAGTTGGAGG CGGAGAAGCA GCAGCTGGTG CAGCGTGGTG AGCAGGCCCT GCAGCAGCAC 
AGCGAGGAGC TGGCCAGGAG GCAGGCACTG GAGACACGGC TGCAGCAGGT GGCAGAGGAG 
CGCCGGGAGG AGACGGCAGC CCTGCAGCGT CAGCTGGCCG AGGTGGCCAA GGGGGAGGAA 
GGAGAGCGCG GCAGGCTGGA GAAGAGGCTG CAGGAGCTGA GCCGGGAGCA CGGGCAGGCC 
TGCCAGCGGC TGCAGGCCGA GCAGGAGAGG GTGGCAGAGC TGGAAGCCCG CGTGGCACGC 
ATGGCCAGCG AGCAGCAGGA GCAGCTGGCC GCCCTCCAGG ACCAGCTGGC CAGAGCTAAG 
GAGAAGGAAG GCAAGGAGCA GGAAAAGCTG GCGAGCGCCG AGCGCGTGGG GAAGTTGGAG 
GCGGAGGTGC GGAAGGCGAG CGAAGCGCTC GAGGCCGTCT CCAAGGAGCT GTCAGAGGAG 
AGGCTGAAAT TCAAGGAGCT CGAAGCTGTG GCCACGCAGG AGGCCAGCGA GGTGGCCCGG 
CTGAGCGCGG CGCTGGAGGA GGCGGCACGG GAGCACGGGC AGGAGCTGGC ACGGCGCGAG 
GAGGAGGCAA AGCGCCTGCA GAAGGAGCTG GAGGACGCCA AGGCCGATTG CGCCGCCGAG 
AAAGCACGCA AGGTAGAGCT GGAGGTACAG CTGCAGAACT CCATCAACGA GCAGCGGGTG 
GAGCGCTCGG CGCACCAGCA GGAACTGGCG CGGTCCCTGG AGCTGATCGA GGAGAAGGAG 
GGAGAGCTGG ATGAGCTGCG GCTGAAGAAC GTCTCGCGGG GTGAGGAGCT GAGGGACCTG 
CAGAAGACAG TCAGCAAGCT GAAAGGGGAG CTCGCCTCGG TGGAAGCAGT GAAGGAGCGG 
GCGTCCAAGA TGGACAGCGA GCTGCAGGGC TTCCTGGAGG CCGCCCGGAG CCGGGATGCT 
GAGATGGACA GCATCAAGGC AGTGTACGCC AAGGAGGCGT CCCTCAAAAA CCTGGAGGAG 
AAGATCCGCC ACCGAGAGCA GGAGTCTGGC TCCAGCCAAG ACCTCTACCA GGAGAAGCTG 
AAGGAGGCCC AGATGCTGAG CGTGGAAGTG GAGCGGCTGG AGCAGAAGTG CCGGGAGCAG 
CAGGACACCA TCGCTGGGCT GGAGAAAGCC GTGGCTGAAG GATCCCAGCA GCAACAGGCG 
GAGCTGGAGG CATTGCAGAG GGAAGCAGTG CGGCACCGGG AGACGGCAGC GGAGCTGCAG 
AGGCTGCTGG ATGCCTCGCG CTCAGCCCAG GCCCTGCAGG ATGGCACCGT GGAGAGCCTG 
AGGAAGGAGC TGCAGGACAA GAGCAAGGAG CTGGCCCAGA GCAAAATGGC TGTGGCTGCT 
GCCGAGAAGG AGGTGGCATC CCTGCGTGCT GCGGCGCAGG AGAAGGGCCA CCTGGAGGAG 
GGCTGGAAGG AGCAGCTGTC GCAATGCATC CAGGAGCTGG AGAGGAAGAA CAGCCTGCTG 
GGCAGCCTGG AGCATGAGGT GTCCATCCTG CACCGGCAGC TCACCGAGAA GGAGGGCGAG 
AGCAAGGAGC TGAAACGCCT GATCATGGCC GAGTCGGAGA AGAGCAAGAA GCTGGAGGAG 
AGGCTGCGGG TGCTGCAGAC CGAGATGGCC ACGGCCGCAT CGCGGGCGGC CGAACGCTGC 
TCGCTGATGA AGGTGGAGGT GCAACGGTGC CAGGAGGAGA TGGAGAAGCA ACGGATGACC 
ATCGAGGCAC TGAAGAGGGA CCGGCACTGC CAGAGCGAGC GCGAGGAGGA GCTGCGGCAG 
GAGGTGAAGG TCTGCCAGGA CAAGTTCTTC CAGAAGGAGC AGCTCCTCTC CTCCCTGCAG 
CAGGAGCTGG GCAGCGCCCA GGCGTTGGCC GGAGAGGTGG TGCCGCTGAA ACACCTGTGC 
CAGCAGCTGC AAGCTGAGCG TGCCTCGCTG GAGAGCAAGC ACCGCGAGGA CCTGGAGCAA 
AGGGCAAAGG CCACCACTGC GCTGCAGGCG GAGCTGGCCC GGGCCAGGGT GGAGGTGGCC 
GAGCTGCCGG CCCTGCGTGA CAGAGCAGCT GAGCAGGAGC GTGCGCTGCA GAGGCTGCAG 
AAGGAGACAG CGAGCTCCAG CGAGAGGCTG GCAGCCCTGC AAGCAGCCAA CAGCCGGCTG 
GCTGAGGAGA ACCGTGCGCT GAGTGAGAGC GTGAGCCGCG GGCAGCAGCG CCTGGATGCT 
GAGCTGGGGC AGGCAAGGGA GAAGCACACA CGGGAGCTGG AGTGCGTGAG GCTGGAGGCG 
GAGAAGGCGA TGGCCAGCAG CAGGCAGGAG GCCGAGGAGG CGGCCAGGAA GCTGGAGGCG 
ATGAGCAATA AGTATGAAAA CACCAAGGTA AAGGTGCTGG AGGAGAGGCA GAAGTTCCAG 
GAGGAGAGGC AGAAGCTGAT GGCGCAGGTG GAGCAGCTAG AGGTATTTCA GAAAGAACAA 
GCAAAGCAGG TGGAGGAGCT GAATAAGAAG CTGGTGCAGC ACGAGAAGGC CACGCGGAGC 
CAGCAGCAGA GAGTTAAGGT GCTGGAAGGA GAGCTGCAGA GCGAGGCCAC GCGCCAGCAG 
GAGAGGGTGG CAGAGCTGGA GGAGCAGCTG GCACAGAAGG AGCAGGCAGC TGAGCACTAC 
AAAGCCCAGA TGGAAAAGGC GAAAACTCAC TACGACGCAA AGAAGCAGCA GAACCAGGAC 
CTGGCAGAGA AGCTGAAGGC CATGGAGCAG CTGCAGAAGG AGAACGCGGA GCTGAGGACG 
GAGTCGGAGC GCTTGGCCAA GGAGCTGCAG CAGAGCGTGC TGCAGGCCAA GGAGGCCGAG 
CTGAGCTGCA GGGAGCTGAG CGGGCAGGTC CGCAGTCTGG AGACACAGCT GGAATTCGCC 
GACCAGCAGC TTCGCGAGCT CGGCAAGTTC CCAGCCCCCA AGGCCACGCT GAAGGAACCG 
GAGAGCTTCC GCCAGAACCC CTCCGACCTC AGCACTGACA GCCTGGACCT CAGCCTGGAC 
GAGGGGCAGC CCCTCAACTC CACCAGGAAA GCCACCCGCT CGCACTCGGA CGGCTCAGCC 
GCTCCGGGTG GCACAGAGCC ACGCGCATCC CAGCGGCTGC CAAGGAAGGT GGAGTCATTG 
GAGAGCCTCT ACTTCACACC CATCGCCAGC CGCACGCAGC CCAAGCTGGA GAGCAGCGCC 
GGTTCTCTGG CCGACGTCTC ACTGGAGTCG GGCTGCAGGA CGCGCTCGGG GCGCCGCCGC 
ACCACGCAGA TCATCAACAT CACCATGACC AAGAAAGAGA CCACCACGGA GGAGCCGGGC 
GGCGCGGACG CGTCCTTCAG CAGCATCCTC TCTGAGCAGC CCCAGAAAGC CGCCCCGGCG 
CGGGCCCGGC TGCGCTCTGC TCGCTCCTTG GCCAGCTTTC CCTCGCAGGA CTCCCTGAGC 
AAGCTGGACA CCTCCTCCCC TCAGGAGCCA TCAGGCCACG CTGCGCTGCT CAGCCTCCCC 
GGCTACCGGC CGGCCACGCG CAGCTCGCTG CGGCGCTCGC AGGCGGGCAG CAGCTCCAGC 
TTAGGTCGCA GCAGCATCTA CCTGGGGACG TGCCAGGACG AACCGGAGCA GCTGGACGAT 
TGGAACCGCA TCGCCGAGCT GCAGCAGCGC AACCGCGCCT GCCCGCCGCA CCTCAAGACC 
TGCTACCCCC TGGAGAGCCG GCCTTCCGAC GTGCTGGGCA CCATCACGGA CGAGGAGATG 
AAGACGGGCG ACCCGACGGA GACGCTGCGG CGCGCCAGCA TGCAGCCCTT GCAGATTGCC 
GAGGGGGCGG CCACCCGGCG CGGCACGCTG GGAGGGGGGT GGGCCCACGG GGGCATCACC 
ACCCGGCAGC AGCGCAAACG GCTCTCGGAT GAATCCCACC AAGGCCCCGA CACCCCCGAG 
TCCAAGAAGC CTGTCAGCTG CTTCCCACGC CCCCAGACCC CCCGCGAGCG GCGCAGCTCC 
CACCTCAGCC GGCGGAGCGA GCAGCAGGCG CCCAGCAAGC AGCCCGAACG GCGCCAGTCC 
ATGGCCTTCA GCATCCTCAA CACCCCCAAG AAGCTGGGGA ACAGCCTCCT GCGCCGGGCT 
GCCAACAGGA AAACCACCCC CAAGAACTCC CCCCGTGGCA CCGCGCGCCG CTCGCCCCGC 
ATCGCCTCCA CCAAATCCCC CAAGGGCAAG GCCGGCCGCA GAGCGCTGAA GGACACCAAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_015280865.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136351.1 Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

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CloneID	OGa31166
Clone ID Related Accession (Same CDS sequence)	XM_015280865.2 , XM_015280865.1
Accession Version	XM_015280865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTCC ACACCACCAG AGCTGCCGCG CTCCTCGCTT GGGTGAACAG CACCAAGGCG 
TGCGCGGAGC CACTTGGCGA CCTGTCCCAG CTGCAGGACT GCCGTGTTTT CATCCAGATC 
ATCAATAAGA TCCACAGGAC TGAGGAGGGG GAGTCCGTCC TGGAGCAGTC CCTGGCGGAG 
CGAGCAGCCT TCATCTGTGG CTTCCTGCAG AAGCTCTGCA AGCACAAGTC GGCCACGGAG 
AACCTGGTGT CAGCACAGAA GCTGCTGGAA GGAGAAGAGC TGGAGTTGGC CAAGGTAGCC 
GTGCTGCTCC TGTACCACAG CTCCATGAGC AGCAAGAGCC CGCGGGACTG GAATGAGTTC 
GATTACCAGA TCCAGGTCGA GCTGGCCACC ATCCTCAAGT TCGTGCTGGA CCATGAGGAG 
AGCTTGAGTG AGAACCTGGA GGTGTTTCTG CAGCGCAGAG CGCCGCTATC CTCGCCCGGC 
ACATCCAGCA GCAGCTCTGA GGAGCGCTCG CCGGGGCTCT CCCACCCTCA GGTGCGCTTC 
CTGGAGCTGC AGAAGATCGC CTCCTCCTCC TCCAGCATGA ACAACATGCT CCCTGGAACA 
CCAGCCTCAC CTGTGGGGGA TGTCCTGCAG ACCCCCCAGT TCCAGCTGCG GAGGTTGAAG 
AAGCAGCTGG CTATCGAGAG GGAGAACCGG GACGAGCTGG AGATGGAGCT GGCAGAGAAC 
CGCAAACTCA TCACTGAGAA GGAGGCTCAG ATCACCATGA TGCAGCACCG CATCGACCGC 
TTGGCTCTGC TCAATGAGAA GCAAGCTGCA GACCAGCTGG AGCCCAGAGA GATGGAGGAG 
CTGAGGGAGA GGAACGAGAG CCTGCTGGTG CGCTTGCACG AGGCCCTGAA GCAGTGCCAG 
GATCTGAAGA CCGAGAAGGG CCAGATGGAC CGCAAGATCA ACCAGCTCTC CGAGGAGAAC 
GGGGACCTCT CCTTCAAGCT GAGGGAGATC GCCAGCCACC TGGTGCAGCT GCAGGATGCC 
CTGAATGAGC TCTCCGAGGA GCACAACTCT GCGCTGATGC AGTCCCAAGC AAAGCAGGCG 
CAGCTGGAGG GCGAGCTGCG GGCTGTGCTG CAGGAGAAGA AATGCCTAGA AGAGAAGGTT 
GAGATCCTTC AGGGGAAGAT TTCCCTGCTG GAAGACCAGC TGGTCAAGCT GGGGGACTGC 
AGCACGCAGG AAAAGGGAGA GGTCATGGGC GACGTCCTGA AGCTGGAAGA GCTCAAGCAG 
GAGGTGTCCA GCCTCACCAC CAAAGGCATT GAGCTCCAAG CCTCCGTCCT GCGGCTGGAG 
GAAGAGAAGA GCCAGCGGGA TGCAGCCCTG CAGGCGGAGC GCAACCGCTT CGAGATGGAG 
AAGCTCCAGC TTGGCACCCA CATCTCCAAC CTGCAGAGCT CCGTCTCCGA GCTGCACCTG 
GCCAAGGAGA AGCTGGAGCA GGAGCTGCGG GCGCAGGAGG CGCGCCTGAC GGCCCAGGCA 
GACGCACTGA CAGCAGAGGT GGGCAAGCTG AGCGGATTCC TGCAGCAGCA GGAGAAGGAG 
GCAGCTGAGC TGCGGATGCA GGCAGAGCAG GAGCGGGCGC AGGCGGAGGA GCTGCGGCAC 
CGGGATATGG CCTCACAGGA GGCTCTCAGC GAGCTGAGCC GGCGTGTGGA GCAGCTGGGT 
GCCAGCCTGA AGCACAGCGA GGAGGAGCTG GTGCGGGTCA CGCAGGAGGC GGAAGGGAAA 
GCCAGACAGC TCGGGGCTGA ACACGAGAAA GCTGTCCAGG AGAGGGATGC AGCCCTGCAG 
CAGCTGCAGC ACTTGCAGCA AGCCAAGGAA AAGCAGCTGG CGGCCCTGAG TAGCCAACTA 
CAGAGCCTGG AGGCAGCCAG CAAAGCTTCG TCCATGGAAA TGCAGCAGGA GAAGGCTGAG 
TTGAATCAGA AGGTCCAAGA GCTGCAAGCC CGCGTTCTTG AGCTCAGCGC ACAGTGCCAG 
CAGAGCTCAG CCCAAGCAGG GAGTGCTGAG ACGCTGCGAG CCCAGTTGCG GGAGCTGGAG 
GGCAAGCTGA AGGACAGCCA GCAGAAGCTG GCTGACAAGG AGAAGGTGGC CAAGGAGAAC 
ACGCGCCTGC AGGAGCGGCT GCTCTTCCTG GAGGAGTCGG TGCGCAACAC TGAGGGCATC 
CTGGAGGACG AGAAGAGGAG AGCAGCGGAG AGCCTGGAGG GGAATCTGGC CCGGATCGCA 
GAGTTGGAGG CGGAGAAGCA GCAGCTGGTG CAGCGTGGTG AGCAGGCCCT GCAGCAGCAC 
AGCGAGGAGC TGGCCAGGAG GCAGGCGCTG GAGACACGGC TGCAGCAGGT GGCAGAGGAG 
CGCCGGGAGG AGACGGCAGC CCTGCAGCGT CAGCTGGCCG AGGTGGCCAA GGGGGAGGAA 
GGAGAGCGTG GCAGGCTGGA GAAGAGGCTG CAGGAGCTGA GCCGGGAGCA CGGGCAGGCC 
TGCCAGCGGC TGCAGGCCGA GCAGGAGAGG GTGGCAGAGC TGGAAGCCCG CGTGGCACGC 
ATGGCCAGCG AGCAGCAGGA GCAGCTGGCC GCCCTCCAGG ACCAGCTGGC CAGAGCTAAG 
GAGAAGGAAG GCAAGGAGCA GGAAAAGCTG GCGAGCGCCG AGCGCGTGGG GAAGTTGGAG 
GCGGAGGTGC GGAAGGCGAG TGAAGCGCTC GAGGCCGTCT CCAAGGAGCT GTCAGAGGAG 
AGGCTGAAAT CCAAGGAGCT CGAAGCTGTG GCCACGCAGG AGGCCAGCGA GGTGGCCCGG 
CTGAGCGCGG CGCTGGAGGA GGCGGCACGG GAGCACGGGC AGGAGCTGGC ACGGCGTGAG 
GAGGAGGCAA AGCGCCTGCA GAAGGAGCTG GAGGACGCCA AGGCCGATTG CGCCGCCGAG 
AAAGCACGCA AGGTAGAGCT GGAGGTCCAG CTGCAGAACT CCATCAACGA GCAGCGGGTG 
GAGCGCTCGG CGCACCAGCA GGAACTGGCG CGGTCCCTGG AGCTGATCGA GGAGAAGGAG 
GGAGAGCTGG ATGAGCTGCG GCTGAAGAAC GTCTCGCGGG GTGAGGAGCT GAGGGACCTG 
CAGAAGACAG TCAGCAAGCT GAAAGGGGAG CTCGCCTCGG TGGAAGCAGT GAAGGAGCGG 
GCGTCCAAGA TGGACAGCGA GCTGCAGGGC TTCCTGGAGG CCGCCCGGAG CCGGGATGCT 
GAGATGGACA GCATCAAGGC AGTGTACGCC AAGGAGGCGT CCCTCAAAAA CCTGGAGGAG 
AAGATCCGCC ACCGAGAGCA GGAGTCTGGC TCCAGCCAAG ACCTCTACCA GGAGAAGCTG 
AAGGAGGCCC AGATGCTGAG CGTGGAAGTG GAGCGGCTGG AGCAGAAGTG CCGGGAGCAG 
CAGGACACCA TCGCTGGGCT GGAGAAAGCC GTGGCTGAAG GATCCCAGCA GCAACAGGCG 
GAGCTGGAGG CATCGCAGAG GGAAGCAGTG CGGCACCGGG AGACGGCAGC GGAGCTGCAG 
AGGCTGCTGG ATGCCTCGCG CTCAGCCCAG GCCCTGCAGG ATGGCACCGT GGAGAGCCTG 
AGGAAGGAGC TGCAGGACAA GAGCAAGGAG CTGGCCCAGA GCAAAACGGC TGTGGCTGCT 
GCCGAGAAGG AGGTGGCATC CCTGCGTGCT GCGGCGCAGG AGAAGGGCCA CCTGGAGGAG 
GGCTGGAAGG AGCAGCTGTC GCAATGCATC CAGGAGCTGG AGAGGAAGAA CAGCCTGCTG 
GGCAGCCTGG AGCACGAGGT GTCCATCCTG CACCGGCAGC TCACCGAGAA GGAGGGCGAG 
AGCAAGGAGC TGAAACGCCT GATCATGGCC GAGTCGGAGA AGAGCAAGAA GCTGGAGGAG 
AGGCTGCGGG TGCTGCAGAC CGAGATGGCC ACGGCCGCAT CGCGGGCGGC CGAACGCTGC 
TCGCTGATGA AGGTGGAGGT GCAACGGTGC CAGGAGGAGA TGGAGAAGCA ACGGATGACC 
ATCGAGGCAC TGAAGAGGGA CCGGCACTGC CAGAGCGAGC GCGAGGAGGA GCTGCGGCAG 
GAGGTGAAGG TCTGCCAGGA CAAGTTCTTC CAGAAGGAGC AGCTCCTCTC CTCCCTGCAG 
CAGGAGCTGG GCAGCGCCCA GGCGTTGGCC GGAGAGGTGG TGCCGCTGAA ACACCTGTGC 
CAGCAGCTGC AAGCTGAGCG TGCCTCGCTG GAGAGCAAGC ACCGCGAGGA CCTGGAGCAA 
AGGGCAAAGG CCACCACTGC GCTGCAGGCG GAGCTGGCCC GGGCCAGGGT GGAGGTGGCC 
GAGCTGCCGG CCCTGCGTGA CAGAGCAGCT GAGCAGGAGC GTGCGCTGCA GAGGCTGCAG 
AAGGAGACAG CGAGCTCCAG CGAGAGGCTG GCAGCCCTGC AAGCAGCCAA CAGCCGGCTG 
GCTGAGGAGA ACCGTGCGCT GAGTGAGAGC GTGAGCCGCG GGCAGCAGCG CCTGGATGCT 
GAGCTGGGGC AGGCAAGGGA GAAGCACACA CGGGAGCTGG AGTGCGTGAG GCTGGAGGCG 
GAGAAGGCGA TGGCCAGCAG CAGGCAGGAG GCCGAGGAGG CGGCCAGGAA GCTGGAGGCG 
ATGAGCAATA AGTATGAAAA CACCAAGGTA AAGGTGCTGG AGGAGAGGCA GAAGTTCCAG 
GAGGAGAGGC AGAAGCTGAT GGCGCAGGTG GAGCAGCTAG AGGTATTTCA GAAAGAACAA 
GCAAAGCAGG TGGAGGAGCT GAATAAGAAG CTGGTGCAGC ACGAGAAGGC CACGCGGAGC 
CAGCAACAGA GAGTTAAGGT GCTGGAAGGA GAGCTGCAGA GCGAGGCCAC GCGCCAGCAG 
GAGAGGGTGG CAGAGCTGGA GGAGCAGCTG GCACAGAAGG AGCAGGCAGC TGAGCACTAC 
AAAGCCCAGA TGGAAAAGGC GAAAACTCAC TACGACGCAA AGAAGCAGCA GAACCAGGAC 
CTGGCAGAGA AGCTGAAGGC CATGGAGCAG CTGCAGAAGG AGAACGCGGA GCTGAGGACG 
GAGTCGGAGC GCTTGGCCAA GGAGCTGCAG CAGAGCGTGC TGCAGGCCAA GGAGGCCGAG 
CTGAGCTGCA GGGAGCTGAG CGGGCAGGTC CGCAGTCTGG AGACACAGCT GGAATTCGCC 
GACCAGCAGC TTCGCGAGCT CGGCAAGTTC CCAGCCCCCA AGGCCACGCT GAAGGAACCG 
GAGAGCTTCC GCCAGAACCC CTCCGACCTC AGCACTGACA GCCTGGACCT CAGCCTGGAC 
GAGGGGCAGC CCCTCAACTC CACCAGGAAA GCCACCCGCT CGCACTCGGA CGGCTCAGCC 
GCTCCGGGTG GCACAGAGCC ACGCGCATCC CAGCGGCTGC CAAGGAAGGT GGAGTCATTG 
GAGAGCCTCT ACTTCACACC CATCGCCAGC CGCACGCAGC CCAAGCTGGA GAGCAGCGCC 
GGTTCTCTGG CCGACGTCTC GCTGGAGTCG GGCTGCAGGA CGCGCTCGGG GCGCCGCCGC 
ACCACACAGA TCATCAACAT CACCATGACC AAGAAAGAGA CCACCACGGA GGAGCCGGGC 
GGCGCGGACG CGTCCTTCAG CAGCATCCTC TCCGAGCAGC CCCAGAAAGC CGCCCCGGCG 
CGGGCCCGGC TGCGCTCTGC TCGCTCCTTG GCCAGCTTTC CCTCGCAGGA CTCCCTGAGC 
AAGCTGGACA CCTCCTCCCC TCAGGAGCCA TCAGGCCACG CTGCGCTGCT CAGCCTCCCC 
GGCTACCGGC CGGCCACGCG CAGCTCGCTG CGGCGCTCGC AGGCGGGCAG CAGCTCCAGC 
TTAGGTCGCA GCAGCATCTA CCTGGGGACG TGCCAGGACG AACCGGAGCA GCTGGACGAT 
TGGAACCGCA TCGCCGAGCT GCAGCAGCGC AACCGCGCCT GCCCGCCGCA CCTCAAGACC 
TGCTACCCCC TGGAGAGCCG GCCTTCCGAC GTGCTGGGCA CCATCACGGA CGAGGAGATG 
AAGACGGGCG ACCCGACGGA GACGCTGCGG CGCGCCAGCA TGCAGCCCTC GCAGATTGCC 
GAGGGGGCGG CCGCCCGGCG CGGCATGCTG GGAGGGGGGT GGGCCCACGG GGGCATCACC 
ACCCGGCAGC AGCGCAAACG GCTCTCGGAT GAATCCCACC AAGGCCCCGA CACCCCCGAG 
TCCAAGAAGC CTGTCAGCTG CTTCCCACGC CCCCAGACCC CCCGCGAGCG GCGCAGCTCC 
CACCTCGGCC GGCGGAGCGA GCAGCAGGCG CCCAGCAAGC AGCCCGAACG GCGCCAGTCC 
ATGGCCTTCA GCATCCTCAA CACCCCCAAG AAGCTGGGGA ACAGCCTCCT GCGCCGGGCT 
GCCAACAGGA AAACCACCCC CAAGAACTCC CCCCGTGGCA CCGCGCGCCG CTCGCCCCGC 
ATCGCCTCCA CCAAATCCCC CAAGGGCAAG GCCGGCCGCA GAGCGCTGAA GGACACCAAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136351.1
CDS	496..6921
Translation	MPLHTTRAAALLAWVNSTKACAEPLGDLSQLQDCRVFIQIINKIHRTEEGESVLEQSLAERAAFICGFLQKLCKHKSATENLVSAQKLLEGEELELAKVAVLLLYHSSMSSKSPRDWNEFDYQIQVELATILKFVLDHEESLSENLEVFLQRRAPLSSPGTSSSSSEERSPGLSHPQVRFLELQKIASSSSSMNNMLPGTPASPVGDVLQTPQFQLRRLKKQLAIERENRDELEMELAENRKLITEKEAQITMMQHRIDRLALLNEKQAADQLEPREMEELRERNESLLVRLHEALKQCQDLKTEKGQMDRKINQLSEENGDLSFKLREIASHLVQLQDALNELSEEHNSALMQSQAKQAQLEGELRAVLQEKKCLEEKVEILQGKISLLEDQLVKLGDCSTQEKGEVMGDVLKLEELKQEVSSLTTKGIELQASVLRLEEEKSQRDAALQAERNRFEMEKLQLGTHISNLQSSVSELHLAKEKLEQELRAQEARLTAQADALTAEVGKLSGFLQQQEKEAAELRMQAEQERAQAEELRHRDMASQEALSELSRRVEQLGASLKHSEEELVRVTQEAEGKARQLGAEHEKAVQERDAALQQLQHLQQAKEKQLAALSSQLQSLEAASKASSMEMQQEKAELNQKVQELQARVLELSAQCQQSSAQAGSAETLRAQLRELEGKLKDSQQKLADKEKVAKENTRLQERLLFLEESVRNTEGILEDEKRRAAESLEGNLARIAELEAEKQQLVQRGEQALQQHSEELARRQALETRLQQVAEERREETAALQRQLAEVAKGEEGERGRLEKRLQELSREHGQACQRLQAEQERVAELEARVARMASEQQEQLAALQDQLARAKEKEGKEQEKLASAERVGKLEAEVRKASEALEAVSKELSEERLKSKELEAVATQEASEVARLSAALEEAAREHGQELARREEEAKRLQKELEDAKADCAAEKARKVELEVQLQNSINEQRVERSAHQQELARSLELIEEKEGELDELRLKNVSRGEELRDLQKTVSKLKGELASVEAVKERASKMDSELQGFLEAARSRDAEMDSIKAVYAKEASLKNLEEKIRHREQESGSSQDLYQEKLKEAQMLSVEVERLEQKCREQQDTIAGLEKAVAEGSQQQQAELEASQREAVRHRETAAELQRLLDASRSAQALQDGTVESLRKELQDKSKELAQSKTAVAAAEKEVASLRAAAQEKGHLEEGWKEQLSQCIQELERKNSLLGSLEHEVSILHRQLTEKEGESKELKRLIMAESEKSKKLEERLRVLQTEMATAASRAAERCSLMKVEVQRCQEEMEKQRMTIEALKRDRHCQSEREEELRQEVKVCQDKFFQKEQLLSSLQQELGSAQALAGEVVPLKHLCQQLQAERASLESKHREDLEQRAKATTALQAELARARVEVAELPALRDRAAEQERALQRLQKETASSSERLAALQAANSRLAEENRALSESVSRGQQRLDAELGQAREKHTRELECVRLEAEKAMASSRQEAEEAARKLEAMSNKYENTKVKVLEERQKFQEERQKLMAQVEQLEVFQKEQAKQVEELNKKLVQHEKATRSQQQRVKVLEGELQSEATRQQERVAELEEQLAQKEQAAEHYKAQMEKAKTHYDAKKQQNQDLAEKLKAMEQLQKENAELRTESERLAKELQQSVLQAKEAELSCRELSGQVRSLETQLEFADQQLRELGKFPAPKATLKEPESFRQNPSDLSTDSLDLSLDEGQPLNSTRKATRSHSDGSAAPGGTEPRASQRLPRKVESLESLYFTPIASRTQPKLESSAGSLADVSLESGCRTRSGRRRTTQIINITMTKKETTTEEPGGADASFSSILSEQPQKAAPARARLRSARSLASFPSQDSLSKLDTSSPQEPSGHAALLSLPGYRPATRSSLRRSQAGSSSSLGRSSIYLGTCQDEPEQLDDWNRIAELQQRNRACPPHLKTCYPLESRPSDVLGTITDEEMKTGDPTETLRRASMQPSQIAEGAAARRGMLGGGWAHGGITTRQQRKRLSDESHQGPDTPESKKPVSCFPRPQTPRERRSSHLGRRSEQQAPSKQPERRQSMAFSILNTPKKLGNSLLRRAANRKTTPKNSPRGTARRSPRIASTKSPKGKAGRRALKDTKF

Target ORF information:

RefSeq Version	XM_015280865.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280865.2

ORF Insert Sequence:

ATGCCGCTCC ACACCACCAG AGCTGCCGCG CTCCTCGCTT GGGTGAACAG CACCAAGGCG 
TGCGCGGAGC CACTTGGCGA CCTGTCCCAG CTGCAGGACT GCCGTGTTTT CATCCAGATC 
ATCAATAAGA TCCACAGGAC TGAGGAGGGG GAGTCCGTCC TGGAGCAGTC CCTGGCGGAG 
CGAGCAGCCT TCATCTGTGG CTTCCTGCAG AAGCTCTGCA AGCACAAGTC GGCCACGGAG 
AACCTGGTGT CAGCACAGAA GCTGCTGGAA GGAGAAGAGC TGGAGTTGGC CAAGGTAGCC 
GTGCTGCTCC TGTACCACAG CTCCATGAGC AGCAAGAGCC CGCGGGACTG GAATGAGTTC 
GATTACCAGA TCCAGGTCGA GCTGGCCACC ATCCTCAAGT TCGTGCTGGA CCATGAGGAG 
AGCTTGAGTG AGAACCTGGA GGTGTTTCTG CAGCGCAGAG CGCCGCTATC CTCGCCCGGC 
ACATCCAGCA GCAGCTCTGA GGAGCGCTCG CCGGGGCTCT CCCACCCTCA GGTGCGCTTC 
CTGGAGCTGC AGAAGATCGC CTCCTCCTCC TCCAGCATGA ACAACATGCT CCCTGGAACA 
CCAGCCTCAC CTGTGGGGGA TGTCCTGCAG ACCCCCCAGT TCCAGCTGCG GAGGTTGAAG 
AAGCAGCTGG CTATCGAGAG GGAGAACCGG GACGAGCTGG AGATGGAGCT GGCAGAGAAC 
CGCAAACTCA TCACTGAGAA GGAGGCTCAG ATCACCATGA TGCAGCACCG CATCGACCGC 
TTGGCTCTGC TCAATGAGAA GCAAGCTGCA GACCAGCTGG AGCCCAGAGA GATGGAGGAG 
CTGAGGGAGA GGAACGAGAG CCTGCTGGTG CGCTTGCACG AGGCCCTGAA GCAGTGCCAG 
GATCTGAAGA CCGAGAAGGG CCAGATGGAC CGCAAGATCA ACCAGCTCTC CGAGGAGAAC 
GGGGACCTCT CCTTCAAGCT GAGGGAGATC GCCAGCCACC TGGTGCAGCT GCAGGATGCC 
CTGAATGAGC TCTCCGAGGA GCACAACTCT GCGCTGATGC AGTCCCAAGC AAAGCAGGCG 
CAGCTGGAGG GCGAGCTGCG GGCTGTGCTG CAGGAGAAGA AATGCCTAGA AGAGAAGGTT 
GAGATCCTTC AGGGGAAGAT TTCCCTGCTG GAAGACCAGC TGGTCAAGCT GGGGGACTGC 
AGCACGCAGG AAAAGGGAGA GGTCATGGGC GACGTCCTGA AGCTGGAAGA GCTCAAGCAG 
GAGGTGTCCA GCCTCACCAC CAAAGGCATT GAGCTCCAAG CCTCCGTCCT GCGGCTGGAG 
GAAGAGAAGA GCCAGCGGGA TGCAGCCCTG CAGGCGGAGC GCAACCGCTT CGAGATGGAG 
AAGCTCCAGC TTGGCACCCA CATCTCCAAC CTGCAGAGCT CCGTCTCCGA GCTGCACCTG 
GCCAAGGAGA AGCTGGAGCA GGAGCTGCGG GCGCAGGAGG CGCGCCTGAC GGCCCAGGCA 
GACGCACTGA CAGCAGAGGT GGGCAAGCTG AGCGGATTCC TGCAGCAGCA GGAGAAGGAG 
GCAGCTGAGC TGCGGATGCA GGCAGAGCAG GAGCGGGCGC AGGCGGAGGA GCTGCGGCAC 
CGGGATATGG CCTCACAGGA GGCTCTCAGC GAGCTGAGCC GGCGTGTGGA GCAGCTGGGT 
GCCAGCCTGA AGCACAGCGA GGAGGAGCTG GTGCGGGTCA CGCAGGAGGC GGAAGGGAAA 
GCCAGACAGC TCGGGGCTGA ACACGAGAAA GCTGTCCAGG AGAGGGATGC AGCCCTGCAG 
CAGCTGCAGC ACTTGCAGCA AGCCAAGGAA AAGCAGCTGG CGGCCCTGAG TAGCCAACTA 
CAGAGCCTGG AGGCAGCCAG CAAAGCTTCG TCCATGGAAA TGCAGCAGGA GAAGGCTGAG 
TTGAATCAGA AGGTCCAAGA GCTGCAAGCC CGCGTTCTTG AGCTCAGCGC ACAGTGCCAG 
CAGAGCTCAG CCCAAGCAGG GAGTGCTGAG ACGCTGCGAG CCCAGTTGCG GGAGCTGGAG 
GGCAAGCTGA AGGACAGCCA GCAGAAGCTG GCTGACAAGG AGAAGGTGGC CAAGGAGAAC 
ACGCGCCTGC AGGAGCGGCT GCTCTTCCTG GAGGAGTCGG TGCGCAACAC TGAGGGCATC 
CTGGAGGACG AGAAGAGGAG AGCAGCGGAG AGCCTGGAGG GGAATCTGGC CCGGATCGCA 
GAGTTGGAGG CGGAGAAGCA GCAGCTGGTG CAGCGTGGTG AGCAGGCCCT GCAGCAGCAC 
AGCGAGGAGC TGGCCAGGAG GCAGGCGCTG GAGACACGGC TGCAGCAGGT GGCAGAGGAG 
CGCCGGGAGG AGACGGCAGC CCTGCAGCGT CAGCTGGCCG AGGTGGCCAA GGGGGAGGAA 
GGAGAGCGTG GCAGGCTGGA GAAGAGGCTG CAGGAGCTGA GCCGGGAGCA CGGGCAGGCC 
TGCCAGCGGC TGCAGGCCGA GCAGGAGAGG GTGGCAGAGC TGGAAGCCCG CGTGGCACGC 
ATGGCCAGCG AGCAGCAGGA GCAGCTGGCC GCCCTCCAGG ACCAGCTGGC CAGAGCTAAG 
GAGAAGGAAG GCAAGGAGCA GGAAAAGCTG GCGAGCGCCG AGCGCGTGGG GAAGTTGGAG 
GCGGAGGTGC GGAAGGCGAG TGAAGCGCTC GAGGCCGTCT CCAAGGAGCT GTCAGAGGAG 
AGGCTGAAAT CCAAGGAGCT CGAAGCTGTG GCCACGCAGG AGGCCAGCGA GGTGGCCCGG 
CTGAGCGCGG CGCTGGAGGA GGCGGCACGG GAGCACGGGC AGGAGCTGGC ACGGCGTGAG 
GAGGAGGCAA AGCGCCTGCA GAAGGAGCTG GAGGACGCCA AGGCCGATTG CGCCGCCGAG 
AAAGCACGCA AGGTAGAGCT GGAGGTCCAG CTGCAGAACT CCATCAACGA GCAGCGGGTG 
GAGCGCTCGG CGCACCAGCA GGAACTGGCG CGGTCCCTGG AGCTGATCGA GGAGAAGGAG 
GGAGAGCTGG ATGAGCTGCG GCTGAAGAAC GTCTCGCGGG GTGAGGAGCT GAGGGACCTG 
CAGAAGACAG TCAGCAAGCT GAAAGGGGAG CTCGCCTCGG TGGAAGCAGT GAAGGAGCGG 
GCGTCCAAGA TGGACAGCGA GCTGCAGGGC TTCCTGGAGG CCGCCCGGAG CCGGGATGCT 
GAGATGGACA GCATCAAGGC AGTGTACGCC AAGGAGGCGT CCCTCAAAAA CCTGGAGGAG 
AAGATCCGCC ACCGAGAGCA GGAGTCTGGC TCCAGCCAAG ACCTCTACCA GGAGAAGCTG 
AAGGAGGCCC AGATGCTGAG CGTGGAAGTG GAGCGGCTGG AGCAGAAGTG CCGGGAGCAG 
CAGGACACCA TCGCTGGGCT GGAGAAAGCC GTGGCTGAAG GATCCCAGCA GCAACAGGCG 
GAGCTGGAGG CATCGCAGAG GGAAGCAGTG CGGCACCGGG AGACGGCAGC GGAGCTGCAG 
AGGCTGCTGG ATGCCTCGCG CTCAGCCCAG GCCCTGCAGG ATGGCACCGT GGAGAGCCTG 
AGGAAGGAGC TGCAGGACAA GAGCAAGGAG CTGGCCCAGA GCAAAACGGC TGTGGCTGCT 
GCCGAGAAGG AGGTGGCATC CCTGCGTGCT GCGGCGCAGG AGAAGGGCCA CCTGGAGGAG 
GGCTGGAAGG AGCAGCTGTC GCAATGCATC CAGGAGCTGG AGAGGAAGAA CAGCCTGCTG 
GGCAGCCTGG AGCACGAGGT GTCCATCCTG CACCGGCAGC TCACCGAGAA GGAGGGCGAG 
AGCAAGGAGC TGAAACGCCT GATCATGGCC GAGTCGGAGA AGAGCAAGAA GCTGGAGGAG 
AGGCTGCGGG TGCTGCAGAC CGAGATGGCC ACGGCCGCAT CGCGGGCGGC CGAACGCTGC 
TCGCTGATGA AGGTGGAGGT GCAACGGTGC CAGGAGGAGA TGGAGAAGCA ACGGATGACC 
ATCGAGGCAC TGAAGAGGGA CCGGCACTGC CAGAGCGAGC GCGAGGAGGA GCTGCGGCAG 
GAGGTGAAGG TCTGCCAGGA CAAGTTCTTC CAGAAGGAGC AGCTCCTCTC CTCCCTGCAG 
CAGGAGCTGG GCAGCGCCCA GGCGTTGGCC GGAGAGGTGG TGCCGCTGAA ACACCTGTGC 
CAGCAGCTGC AAGCTGAGCG TGCCTCGCTG GAGAGCAAGC ACCGCGAGGA CCTGGAGCAA 
AGGGCAAAGG CCACCACTGC GCTGCAGGCG GAGCTGGCCC GGGCCAGGGT GGAGGTGGCC 
GAGCTGCCGG CCCTGCGTGA CAGAGCAGCT GAGCAGGAGC GTGCGCTGCA GAGGCTGCAG 
AAGGAGACAG CGAGCTCCAG CGAGAGGCTG GCAGCCCTGC AAGCAGCCAA CAGCCGGCTG 
GCTGAGGAGA ACCGTGCGCT GAGTGAGAGC GTGAGCCGCG GGCAGCAGCG CCTGGATGCT 
GAGCTGGGGC AGGCAAGGGA GAAGCACACA CGGGAGCTGG AGTGCGTGAG GCTGGAGGCG 
GAGAAGGCGA TGGCCAGCAG CAGGCAGGAG GCCGAGGAGG CGGCCAGGAA GCTGGAGGCG 
ATGAGCAATA AGTATGAAAA CACCAAGGTA AAGGTGCTGG AGGAGAGGCA GAAGTTCCAG 
GAGGAGAGGC AGAAGCTGAT GGCGCAGGTG GAGCAGCTAG AGGTATTTCA GAAAGAACAA 
GCAAAGCAGG TGGAGGAGCT GAATAAGAAG CTGGTGCAGC ACGAGAAGGC CACGCGGAGC 
CAGCAACAGA GAGTTAAGGT GCTGGAAGGA GAGCTGCAGA GCGAGGCCAC GCGCCAGCAG 
GAGAGGGTGG CAGAGCTGGA GGAGCAGCTG GCACAGAAGG AGCAGGCAGC TGAGCACTAC 
AAAGCCCAGA TGGAAAAGGC GAAAACTCAC TACGACGCAA AGAAGCAGCA GAACCAGGAC 
CTGGCAGAGA AGCTGAAGGC CATGGAGCAG CTGCAGAAGG AGAACGCGGA GCTGAGGACG 
GAGTCGGAGC GCTTGGCCAA GGAGCTGCAG CAGAGCGTGC TGCAGGCCAA GGAGGCCGAG 
CTGAGCTGCA GGGAGCTGAG CGGGCAGGTC CGCAGTCTGG AGACACAGCT GGAATTCGCC 
GACCAGCAGC TTCGCGAGCT CGGCAAGTTC CCAGCCCCCA AGGCCACGCT GAAGGAACCG 
GAGAGCTTCC GCCAGAACCC CTCCGACCTC AGCACTGACA GCCTGGACCT CAGCCTGGAC 
GAGGGGCAGC CCCTCAACTC CACCAGGAAA GCCACCCGCT CGCACTCGGA CGGCTCAGCC 
GCTCCGGGTG GCACAGAGCC ACGCGCATCC CAGCGGCTGC CAAGGAAGGT GGAGTCATTG 
GAGAGCCTCT ACTTCACACC CATCGCCAGC CGCACGCAGC CCAAGCTGGA GAGCAGCGCC 
GGTTCTCTGG CCGACGTCTC GCTGGAGTCG GGCTGCAGGA CGCGCTCGGG GCGCCGCCGC 
ACCACACAGA TCATCAACAT CACCATGACC AAGAAAGAGA CCACCACGGA GGAGCCGGGC 
GGCGCGGACG CGTCCTTCAG CAGCATCCTC TCCGAGCAGC CCCAGAAAGC CGCCCCGGCG 
CGGGCCCGGC TGCGCTCTGC TCGCTCCTTG GCCAGCTTTC CCTCGCAGGA CTCCCTGAGC 
AAGCTGGACA CCTCCTCCCC TCAGGAGCCA TCAGGCCACG CTGCGCTGCT CAGCCTCCCC 
GGCTACCGGC CGGCCACGCG CAGCTCGCTG CGGCGCTCGC AGGCGGGCAG CAGCTCCAGC 
TTAGGTCGCA GCAGCATCTA CCTGGGGACG TGCCAGGACG AACCGGAGCA GCTGGACGAT 
TGGAACCGCA TCGCCGAGCT GCAGCAGCGC AACCGCGCCT GCCCGCCGCA CCTCAAGACC 
TGCTACCCCC TGGAGAGCCG GCCTTCCGAC GTGCTGGGCA CCATCACGGA CGAGGAGATG 
AAGACGGGCG ACCCGACGGA GACGCTGCGG CGCGCCAGCA TGCAGCCCTC GCAGATTGCC 
GAGGGGGCGG CCGCCCGGCG CGGCATGCTG GGAGGGGGGT GGGCCCACGG GGGCATCACC 
ACCCGGCAGC AGCGCAAACG GCTCTCGGAT GAATCCCACC AAGGCCCCGA CACCCCCGAG 
TCCAAGAAGC CTGTCAGCTG CTTCCCACGC CCCCAGACCC CCCGCGAGCG GCGCAGCTCC 
CACCTCGGCC GGCGGAGCGA GCAGCAGGCG CCCAGCAAGC AGCCCGAACG GCGCCAGTCC 
ATGGCCTTCA GCATCCTCAA CACCCCCAAG AAGCTGGGGA ACAGCCTCCT GCGCCGGGCT 
GCCAACAGGA AAACCACCCC CAAGAACTCC CCCCGTGGCA CCGCGCGCCG CTCGCCCCGC 
ATCGCCTCCA CCAAATCCCC CAAGGGCAAG GCCGGCCGCA GAGCGCTGAA GGACACCAAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_015280865.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136351.1 Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

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CloneID	OGa31166
Clone ID Related Accession (Same CDS sequence)	XM_015280865.2 , XM_015280865.1
Accession Version	XM_015280865.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455765.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTCC ACACCACCAG AGCTGCCGCG CTCCTCGCTT GGGTGAACAG CACCAAGGCG 
TGCGCGGAGC CACTTGGCGA CCTGTCCCAG CTGCAGGACT GCCGTGTTTT CATCCAGATC 
ATCAATAAGA TCCACAGGAC TGAGGAGGGG GAGTCCGTCC TGGAGCAGTC CCTGGCGGAG 
CGAGCAGCCT TCATCTGTGG CTTCCTGCAG AAGCTCTGCA AGCACAAGTC GGCCACGGAG 
AACCTGGTGT CAGCACAGAA GCTGCTGGAA GGAGAAGAGC TGGAGTTGGC CAAGGTAGCC 
GTGCTGCTCC TGTACCACAG CTCCATGAGC AGCAAGAGCC CGCGGGACTG GAATGAGTTC 
GATTACCAGA TCCAGGTCGA GCTGGCCACC ATCCTCAAGT TCGTGCTGGA CCATGAGGAG 
AGCTTGAGTG AGAACCTGGA GGTGTTTCTG CAGCGCAGAG CGCCGCTATC CTCGCCCGGC 
ACATCCAGCA GCAGCTCTGA GGAGCGCTCG CCGGGGCTCT CCCACCCTCA GGTGCGCTTC 
CTGGAGCTGC AGAAGATCGC CTCCTCCTCC TCCAGCATGA ACAACATGCT CCCTGGAACA 
CCAGCCTCAC CTGTGGGGGA TGTCCTGCAG ACCCCCCAGT TCCAGCTGCG GAGGTTGAAG 
AAGCAGCTGG CTATCGAGAG GGAGAACCGG GACGAGCTGG AGATGGAGCT GGCAGAGAAC 
CGCAAACTCA TCACTGAGAA GGAGGCTCAG ATCACCATGA TGCAGCACCG CATCGACCGC 
TTGGCTCTGC TCAATGAGAA GCAAGCTGCA GACCAGCTGG AGCCCAGAGA GATGGAGGAG 
CTGAGGGAGA GGAACGAGAG CCTGCTGGTG CGCTTGCACG AGGCCCTGAA GCAGTGCCAG 
GATCTGAAGA CCGAGAAGGG CCAGATGGAC CGCAAGATCA ACCAGCTCTC CGAGGAGAAC 
GGGGACCTCT CCTTCAAGCT GAGGGAGATC GCCAGCCACC TGGTGCAGCT GCAGGATGCC 
CTGAATGAGC TCTCCGAGGA GCACAACTCT GCGCTGATGC AGTCCCAAGC AAAGCAGGCG 
CAGCTGGAGG GCGAGCTGCG GGCTGTGCTG CAGGAGAAGA AATGCCTAGA AGAGAAGGTT 
GAGATCCTTC AGGGGAAGAT TTCCCTGCTG GAAGACCAGC TGGTCAAGCT GGGGGACTGC 
AGCACGCAGG AAAAGGGAGA GGTCATGGGC GACGTCCTGA AGCTGGAAGA GCTCAAGCAG 
GAGGTGTCCA GCCTCACCAC CAAAGGCATT GAGCTCCAAG CCTCCGTCCT GCGGCTGGAG 
GAAGAGAAGA GCCAGCGGGA TGCAGCCCTG CAGGCGGAGC GCAACCGCTT CGAGATGGAG 
AAGCTCCAGC TTGGCACCCA CATCTCCAAC CTGCAGAGCT CCGTCTCCGA GCTGCACCTG 
GCCAAGGAGA AGCTGGAGCA GGAGCTGCGG GCGCAGGAGG CGCGCCTGAC GGCCCAGGCA 
GACGCACTGA CAGCAGAGGT GGGCAAGCTG AGCGGATTCC TGCAGCAGCA GGAGAAGGAG 
GCAGCTGAGC TGCGGATGCA GGCAGAGCAG GAGCGGGCGC AGGCGGAGGA GCTGCGGCAC 
CGGGATATGG CCTCACAGGA GGCTCTCAGC GAGCTGAGCC GGCGTGTGGA GCAGCTGGGT 
GCCAGCCTGA AGCACAGCGA GGAGGAGCTG GTGCGGGTCA CGCAGGAGGC GGAAGGGAAA 
GCCAGACAGC TCGGGGCTGA ACACGAGAAA GCTGTCCAGG AGAGGGATGC AGCCCTGCAG 
CAGCTGCAGC ACTTGCAGCA AGCCAAGGAA AAGCAGCTGG CGGCCCTGAG TAGCCAACTA 
CAGAGCCTGG AGGCAGCCAG CAAAGCTTCG TCCATGGAAA TGCAGCAGGA GAAGGCTGAG 
TTGAATCAGA AGGTCCAAGA GCTGCAAGCC CGCGTTCTTG AGCTCAGCGC ACAGTGCCAG 
CAGAGCTCAG CCCAAGCAGG GAGTGCTGAG ACGCTGCGAG CCCAGTTGCG GGAGCTGGAG 
GGCAAGCTGA AGGACAGCCA GCAGAAGCTG GCTGACAAGG AGAAGGTGGC CAAGGAGAAC 
ACGCGCCTGC AGGAGCGGCT GCTCTTCCTG GAGGAGTCGG TGCGCAACAC TGAGGGCATC 
CTGGAGGACG AGAAGAGGAG AGCAGCGGAG AGCCTGGAGG GGAATCTGGC CCGGATCGCA 
GAGTTGGAGG CGGAGAAGCA GCAGCTGGTG CAGCGTGGTG AGCAGGCCCT GCAGCAGCAC 
AGCGAGGAGC TGGCCAGGAG GCAGGCGCTG GAGACACGGC TGCAGCAGGT GGCAGAGGAG 
CGCCGGGAGG AGACGGCAGC CCTGCAGCGT CAGCTGGCCG AGGTGGCCAA GGGGGAGGAA 
GGAGAGCGTG GCAGGCTGGA GAAGAGGCTG CAGGAGCTGA GCCGGGAGCA CGGGCAGGCC 
TGCCAGCGGC TGCAGGCCGA GCAGGAGAGG GTGGCAGAGC TGGAAGCCCG CGTGGCACGC 
ATGGCCAGCG AGCAGCAGGA GCAGCTGGCC GCCCTCCAGG ACCAGCTGGC CAGAGCTAAG 
GAGAAGGAAG GCAAGGAGCA GGAAAAGCTG GCGAGCGCCG AGCGCGTGGG GAAGTTGGAG 
GCGGAGGTGC GGAAGGCGAG TGAAGCGCTC GAGGCCGTCT CCAAGGAGCT GTCAGAGGAG 
AGGCTGAAAT CCAAGGAGCT CGAAGCTGTG GCCACGCAGG AGGCCAGCGA GGTGGCCCGG 
CTGAGCGCGG CGCTGGAGGA GGCGGCACGG GAGCACGGGC AGGAGCTGGC ACGGCGTGAG 
GAGGAGGCAA AGCGCCTGCA GAAGGAGCTG GAGGACGCCA AGGCCGATTG CGCCGCCGAG 
AAAGCACGCA AGGTAGAGCT GGAGGTCCAG CTGCAGAACT CCATCAACGA GCAGCGGGTG 
GAGCGCTCGG CGCACCAGCA GGAACTGGCG CGGTCCCTGG AGCTGATCGA GGAGAAGGAG 
GGAGAGCTGG ATGAGCTGCG GCTGAAGAAC GTCTCGCGGG GTGAGGAGCT GAGGGACCTG 
CAGAAGACAG TCAGCAAGCT GAAAGGGGAG CTCGCCTCGG TGGAAGCAGT GAAGGAGCGG 
GCGTCCAAGA TGGACAGCGA GCTGCAGGGC TTCCTGGAGG CCGCCCGGAG CCGGGATGCT 
GAGATGGACA GCATCAAGGC AGTGTACGCC AAGGAGGCGT CCCTCAAAAA CCTGGAGGAG 
AAGATCCGCC ACCGAGAGCA GGAGTCTGGC TCCAGCCAAG ACCTCTACCA GGAGAAGCTG 
AAGGAGGCCC AGATGCTGAG CGTGGAAGTG GAGCGGCTGG AGCAGAAGTG CCGGGAGCAG 
CAGGACACCA TCGCTGGGCT GGAGAAAGCC GTGGCTGAAG GATCCCAGCA GCAACAGGCG 
GAGCTGGAGG CATCGCAGAG GGAAGCAGTG CGGCACCGGG AGACGGCAGC GGAGCTGCAG 
AGGCTGCTGG ATGCCTCGCG CTCAGCCCAG GCCCTGCAGG ATGGCACCGT GGAGAGCCTG 
AGGAAGGAGC TGCAGGACAA GAGCAAGGAG CTGGCCCAGA GCAAAACGGC TGTGGCTGCT 
GCCGAGAAGG AGGTGGCATC CCTGCGTGCT GCGGCGCAGG AGAAGGGCCA CCTGGAGGAG 
GGCTGGAAGG AGCAGCTGTC GCAATGCATC CAGGAGCTGG AGAGGAAGAA CAGCCTGCTG 
GGCAGCCTGG AGCACGAGGT GTCCATCCTG CACCGGCAGC TCACCGAGAA GGAGGGCGAG 
AGCAAGGAGC TGAAACGCCT GATCATGGCC GAGTCGGAGA AGAGCAAGAA GCTGGAGGAG 
AGGCTGCGGG TGCTGCAGAC CGAGATGGCC ACGGCCGCAT CGCGGGCGGC CGAACGCTGC 
TCGCTGATGA AGGTGGAGGT GCAACGGTGC CAGGAGGAGA TGGAGAAGCA ACGGATGACC 
ATCGAGGCAC TGAAGAGGGA CCGGCACTGC CAGAGCGAGC GCGAGGAGGA GCTGCGGCAG 
GAGGTGAAGG TCTGCCAGGA CAAGTTCTTC CAGAAGGAGC AGCTCCTCTC CTCCCTGCAG 
CAGGAGCTGG GCAGCGCCCA GGCGTTGGCC GGAGAGGTGG TGCCGCTGAA ACACCTGTGC 
CAGCAGCTGC AAGCTGAGCG TGCCTCGCTG GAGAGCAAGC ACCGCGAGGA CCTGGAGCAA 
AGGGCAAAGG CCACCACTGC GCTGCAGGCG GAGCTGGCCC GGGCCAGGGT GGAGGTGGCC 
GAGCTGCCGG CCCTGCGTGA CAGAGCAGCT GAGCAGGAGC GTGCGCTGCA GAGGCTGCAG 
AAGGAGACAG CGAGCTCCAG CGAGAGGCTG GCAGCCCTGC AAGCAGCCAA CAGCCGGCTG 
GCTGAGGAGA ACCGTGCGCT GAGTGAGAGC GTGAGCCGCG GGCAGCAGCG CCTGGATGCT 
GAGCTGGGGC AGGCAAGGGA GAAGCACACA CGGGAGCTGG AGTGCGTGAG GCTGGAGGCG 
GAGAAGGCGA TGGCCAGCAG CAGGCAGGAG GCCGAGGAGG CGGCCAGGAA GCTGGAGGCG 
ATGAGCAATA AGTATGAAAA CACCAAGGTA AAGGTGCTGG AGGAGAGGCA GAAGTTCCAG 
GAGGAGAGGC AGAAGCTGAT GGCGCAGGTG GAGCAGCTAG AGGTATTTCA GAAAGAACAA 
GCAAAGCAGG TGGAGGAGCT GAATAAGAAG CTGGTGCAGC ACGAGAAGGC CACGCGGAGC 
CAGCAACAGA GAGTTAAGGT GCTGGAAGGA GAGCTGCAGA GCGAGGCCAC GCGCCAGCAG 
GAGAGGGTGG CAGAGCTGGA GGAGCAGCTG GCACAGAAGG AGCAGGCAGC TGAGCACTAC 
AAAGCCCAGA TGGAAAAGGC GAAAACTCAC TACGACGCAA AGAAGCAGCA GAACCAGGAC 
CTGGCAGAGA AGCTGAAGGC CATGGAGCAG CTGCAGAAGG AGAACGCGGA GCTGAGGACG 
GAGTCGGAGC GCTTGGCCAA GGAGCTGCAG CAGAGCGTGC TGCAGGCCAA GGAGGCCGAG 
CTGAGCTGCA GGGAGCTGAG CGGGCAGGTC CGCAGTCTGG AGACACAGCT GGAATTCGCC 
GACCAGCAGC TTCGCGAGCT CGGCAAGTTC CCAGCCCCCA AGGCCACGCT GAAGGAACCG 
GAGAGCTTCC GCCAGAACCC CTCCGACCTC AGCACTGACA GCCTGGACCT CAGCCTGGAC 
GAGGGGCAGC CCCTCAACTC CACCAGGAAA GCCACCCGCT CGCACTCGGA CGGCTCAGCC 
GCTCCGGGTG GCACAGAGCC ACGCGCATCC CAGCGGCTGC CAAGGAAGGT GGAGTCATTG 
GAGAGCCTCT ACTTCACACC CATCGCCAGC CGCACGCAGC CCAAGCTGGA GAGCAGCGCC 
GGTTCTCTGG CCGACGTCTC GCTGGAGTCG GGCTGCAGGA CGCGCTCGGG GCGCCGCCGC 
ACCACACAGA TCATCAACAT CACCATGACC AAGAAAGAGA CCACCACGGA GGAGCCGGGC 
GGCGCGGACG CGTCCTTCAG CAGCATCCTC TCCGAGCAGC CCCAGAAAGC CGCCCCGGCG 
CGGGCCCGGC TGCGCTCTGC TCGCTCCTTG GCCAGCTTTC CCTCGCAGGA CTCCCTGAGC 
AAGCTGGACA CCTCCTCCCC TCAGGAGCCA TCAGGCCACG CTGCGCTGCT CAGCCTCCCC 
GGCTACCGGC CGGCCACGCG CAGCTCGCTG CGGCGCTCGC AGGCGGGCAG CAGCTCCAGC 
TTAGGTCGCA GCAGCATCTA CCTGGGGACG TGCCAGGACG AACCGGAGCA GCTGGACGAT 
TGGAACCGCA TCGCCGAGCT GCAGCAGCGC AACCGCGCCT GCCCGCCGCA CCTCAAGACC 
TGCTACCCCC TGGAGAGCCG GCCTTCCGAC GTGCTGGGCA CCATCACGGA CGAGGAGATG 
AAGACGGGCG ACCCGACGGA GACGCTGCGG CGCGCCAGCA TGCAGCCCTC GCAGATTGCC 
GAGGGGGCGG CCGCCCGGCG CGGCATGCTG GGAGGGGGGT GGGCCCACGG GGGCATCACC 
ACCCGGCAGC AGCGCAAACG GCTCTCGGAT GAATCCCACC AAGGCCCCGA CACCCCCGAG 
TCCAAGAAGC CTGTCAGCTG CTTCCCACGC CCCCAGACCC CCCGCGAGCG GCGCAGCTCC 
CACCTCGGCC GGCGGAGCGA GCAGCAGGCG CCCAGCAAGC AGCCCGAACG GCGCCAGTCC 
ATGGCCTTCA GCATCCTCAA CACCCCCAAG AAGCTGGGGA ACAGCCTCCT GCGCCGGGCT 
GCCAACAGGA AAACCACCCC CAAGAACTCC CCCCGTGGCA CCGCGCGCCG CTCGCCCCGC 
ATCGCCTCCA CCAAATCCCC CAAGGGCAAG GCCGGCCGCA GAGCGCTGAA GGACACCAAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136351.1
CDS	499..6924
Translation	MPLHTTRAAALLAWVNSTKACAEPLGDLSQLQDCRVFIQIINKIHRTEEGESVLEQSLAERAAFICGFLQKLCKHKSATENLVSAQKLLEGEELELAKVAVLLLYHSSMSSKSPRDWNEFDYQIQVELATILKFVLDHEESLSENLEVFLQRRAPLSSPGTSSSSSEERSPGLSHPQVRFLELQKIASSSSSMNNMLPGTPASPVGDVLQTPQFQLRRLKKQLAIERENRDELEMELAENRKLITEKEAQITMMQHRIDRLALLNEKQAADQLEPREMEELRERNESLLVRLHEALKQCQDLKTEKGQMDRKINQLSEENGDLSFKLREIASHLVQLQDALNELSEEHNSALMQSQAKQAQLEGELRAVLQEKKCLEEKVEILQGKISLLEDQLVKLGDCSTQEKGEVMGDVLKLEELKQEVSSLTTKGIELQASVLRLEEEKSQRDAALQAERNRFEMEKLQLGTHISNLQSSVSELHLAKEKLEQELRAQEARLTAQADALTAEVGKLSGFLQQQEKEAAELRMQAEQERAQAEELRHRDMASQEALSELSRRVEQLGASLKHSEEELVRVTQEAEGKARQLGAEHEKAVQERDAALQQLQHLQQAKEKQLAALSSQLQSLEAASKASSMEMQQEKAELNQKVQELQARVLELSAQCQQSSAQAGSAETLRAQLRELEGKLKDSQQKLADKEKVAKENTRLQERLLFLEESVRNTEGILEDEKRRAAESLEGNLARIAELEAEKQQLVQRGEQALQQHSEELARRQALETRLQQVAEERREETAALQRQLAEVAKGEEGERGRLEKRLQELSREHGQACQRLQAEQERVAELEARVARMASEQQEQLAALQDQLARAKEKEGKEQEKLASAERVGKLEAEVRKASEALEAVSKELSEERLKSKELEAVATQEASEVARLSAALEEAAREHGQELARREEEAKRLQKELEDAKADCAAEKARKVELEVQLQNSINEQRVERSAHQQELARSLELIEEKEGELDELRLKNVSRGEELRDLQKTVSKLKGELASVEAVKERASKMDSELQGFLEAARSRDAEMDSIKAVYAKEASLKNLEEKIRHREQESGSSQDLYQEKLKEAQMLSVEVERLEQKCREQQDTIAGLEKAVAEGSQQQQAELEASQREAVRHRETAAELQRLLDASRSAQALQDGTVESLRKELQDKSKELAQSKTAVAAAEKEVASLRAAAQEKGHLEEGWKEQLSQCIQELERKNSLLGSLEHEVSILHRQLTEKEGESKELKRLIMAESEKSKKLEERLRVLQTEMATAASRAAERCSLMKVEVQRCQEEMEKQRMTIEALKRDRHCQSEREEELRQEVKVCQDKFFQKEQLLSSLQQELGSAQALAGEVVPLKHLCQQLQAERASLESKHREDLEQRAKATTALQAELARARVEVAELPALRDRAAEQERALQRLQKETASSSERLAALQAANSRLAEENRALSESVSRGQQRLDAELGQAREKHTRELECVRLEAEKAMASSRQEAEEAARKLEAMSNKYENTKVKVLEERQKFQEERQKLMAQVEQLEVFQKEQAKQVEELNKKLVQHEKATRSQQQRVKVLEGELQSEATRQQERVAELEEQLAQKEQAAEHYKAQMEKAKTHYDAKKQQNQDLAEKLKAMEQLQKENAELRTESERLAKELQQSVLQAKEAELSCRELSGQVRSLETQLEFADQQLRELGKFPAPKATLKEPESFRQNPSDLSTDSLDLSLDEGQPLNSTRKATRSHSDGSAAPGGTEPRASQRLPRKVESLESLYFTPIASRTQPKLESSAGSLADVSLESGCRTRSGRRRTTQIINITMTKKETTTEEPGGADASFSSILSEQPQKAAPARARLRSARSLASFPSQDSLSKLDTSSPQEPSGHAALLSLPGYRPATRSSLRRSQAGSSSSLGRSSIYLGTCQDEPEQLDDWNRIAELQQRNRACPPHLKTCYPLESRPSDVLGTITDEEMKTGDPTETLRRASMQPSQIAEGAAARRGMLGGGWAHGGITTRQQRKRLSDESHQGPDTPESKKPVSCFPRPQTPRERRSSHLGRRSEQQAPSKQPERRQSMAFSILNTPKKLGNSLLRRAANRKTTPKNSPRGTARRSPRIASTKSPKGKAGRRALKDTKF

Target ORF information:

RefSeq Version	XM_015280865.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015280865.1

ORF Insert Sequence:

ATGCCGCTCC ACACCACCAG AGCTGCCGCG CTCCTCGCTT GGGTGAACAG CACCAAGGCG 
TGCGCGGAGC CACTTGGCGA CCTGTCCCAG CTGCAGGACT GCCGTGTTTT CATCCAGATC 
ATCAATAAGA TCCACAGGAC TGAGGAGGGG GAGTCCGTCC TGGAGCAGTC CCTGGCGGAG 
CGAGCAGCCT TCATCTGTGG CTTCCTGCAG AAGCTCTGCA AGCACAAGTC GGCCACGGAG 
AACCTGGTGT CAGCACAGAA GCTGCTGGAA GGAGAAGAGC TGGAGTTGGC CAAGGTAGCC 
GTGCTGCTCC TGTACCACAG CTCCATGAGC AGCAAGAGCC CGCGGGACTG GAATGAGTTC 
GATTACCAGA TCCAGGTCGA GCTGGCCACC ATCCTCAAGT TCGTGCTGGA CCATGAGGAG 
AGCTTGAGTG AGAACCTGGA GGTGTTTCTG CAGCGCAGAG CGCCGCTATC CTCGCCCGGC 
ACATCCAGCA GCAGCTCTGA GGAGCGCTCG CCGGGGCTCT CCCACCCTCA GGTGCGCTTC 
CTGGAGCTGC AGAAGATCGC CTCCTCCTCC TCCAGCATGA ACAACATGCT CCCTGGAACA 
CCAGCCTCAC CTGTGGGGGA TGTCCTGCAG ACCCCCCAGT TCCAGCTGCG GAGGTTGAAG 
AAGCAGCTGG CTATCGAGAG GGAGAACCGG GACGAGCTGG AGATGGAGCT GGCAGAGAAC 
CGCAAACTCA TCACTGAGAA GGAGGCTCAG ATCACCATGA TGCAGCACCG CATCGACCGC 
TTGGCTCTGC TCAATGAGAA GCAAGCTGCA GACCAGCTGG AGCCCAGAGA GATGGAGGAG 
CTGAGGGAGA GGAACGAGAG CCTGCTGGTG CGCTTGCACG AGGCCCTGAA GCAGTGCCAG 
GATCTGAAGA CCGAGAAGGG CCAGATGGAC CGCAAGATCA ACCAGCTCTC CGAGGAGAAC 
GGGGACCTCT CCTTCAAGCT GAGGGAGATC GCCAGCCACC TGGTGCAGCT GCAGGATGCC 
CTGAATGAGC TCTCCGAGGA GCACAACTCT GCGCTGATGC AGTCCCAAGC AAAGCAGGCG 
CAGCTGGAGG GCGAGCTGCG GGCTGTGCTG CAGGAGAAGA AATGCCTAGA AGAGAAGGTT 
GAGATCCTTC AGGGGAAGAT TTCCCTGCTG GAAGACCAGC TGGTCAAGCT GGGGGACTGC 
AGCACGCAGG AAAAGGGAGA GGTCATGGGC GACGTCCTGA AGCTGGAAGA GCTCAAGCAG 
GAGGTGTCCA GCCTCACCAC CAAAGGCATT GAGCTCCAAG CCTCCGTCCT GCGGCTGGAG 
GAAGAGAAGA GCCAGCGGGA TGCAGCCCTG CAGGCGGAGC GCAACCGCTT CGAGATGGAG 
AAGCTCCAGC TTGGCACCCA CATCTCCAAC CTGCAGAGCT CCGTCTCCGA GCTGCACCTG 
GCCAAGGAGA AGCTGGAGCA GGAGCTGCGG GCGCAGGAGG CGCGCCTGAC GGCCCAGGCA 
GACGCACTGA CAGCAGAGGT GGGCAAGCTG AGCGGATTCC TGCAGCAGCA GGAGAAGGAG 
GCAGCTGAGC TGCGGATGCA GGCAGAGCAG GAGCGGGCGC AGGCGGAGGA GCTGCGGCAC 
CGGGATATGG CCTCACAGGA GGCTCTCAGC GAGCTGAGCC GGCGTGTGGA GCAGCTGGGT 
GCCAGCCTGA AGCACAGCGA GGAGGAGCTG GTGCGGGTCA CGCAGGAGGC GGAAGGGAAA 
GCCAGACAGC TCGGGGCTGA ACACGAGAAA GCTGTCCAGG AGAGGGATGC AGCCCTGCAG 
CAGCTGCAGC ACTTGCAGCA AGCCAAGGAA AAGCAGCTGG CGGCCCTGAG TAGCCAACTA 
CAGAGCCTGG AGGCAGCCAG CAAAGCTTCG TCCATGGAAA TGCAGCAGGA GAAGGCTGAG 
TTGAATCAGA AGGTCCAAGA GCTGCAAGCC CGCGTTCTTG AGCTCAGCGC ACAGTGCCAG 
CAGAGCTCAG CCCAAGCAGG GAGTGCTGAG ACGCTGCGAG CCCAGTTGCG GGAGCTGGAG 
GGCAAGCTGA AGGACAGCCA GCAGAAGCTG GCTGACAAGG AGAAGGTGGC CAAGGAGAAC 
ACGCGCCTGC AGGAGCGGCT GCTCTTCCTG GAGGAGTCGG TGCGCAACAC TGAGGGCATC 
CTGGAGGACG AGAAGAGGAG AGCAGCGGAG AGCCTGGAGG GGAATCTGGC CCGGATCGCA 
GAGTTGGAGG CGGAGAAGCA GCAGCTGGTG CAGCGTGGTG AGCAGGCCCT GCAGCAGCAC 
AGCGAGGAGC TGGCCAGGAG GCAGGCGCTG GAGACACGGC TGCAGCAGGT GGCAGAGGAG 
CGCCGGGAGG AGACGGCAGC CCTGCAGCGT CAGCTGGCCG AGGTGGCCAA GGGGGAGGAA 
GGAGAGCGTG GCAGGCTGGA GAAGAGGCTG CAGGAGCTGA GCCGGGAGCA CGGGCAGGCC 
TGCCAGCGGC TGCAGGCCGA GCAGGAGAGG GTGGCAGAGC TGGAAGCCCG CGTGGCACGC 
ATGGCCAGCG AGCAGCAGGA GCAGCTGGCC GCCCTCCAGG ACCAGCTGGC CAGAGCTAAG 
GAGAAGGAAG GCAAGGAGCA GGAAAAGCTG GCGAGCGCCG AGCGCGTGGG GAAGTTGGAG 
GCGGAGGTGC GGAAGGCGAG TGAAGCGCTC GAGGCCGTCT CCAAGGAGCT GTCAGAGGAG 
AGGCTGAAAT CCAAGGAGCT CGAAGCTGTG GCCACGCAGG AGGCCAGCGA GGTGGCCCGG 
CTGAGCGCGG CGCTGGAGGA GGCGGCACGG GAGCACGGGC AGGAGCTGGC ACGGCGTGAG 
GAGGAGGCAA AGCGCCTGCA GAAGGAGCTG GAGGACGCCA AGGCCGATTG CGCCGCCGAG 
AAAGCACGCA AGGTAGAGCT GGAGGTCCAG CTGCAGAACT CCATCAACGA GCAGCGGGTG 
GAGCGCTCGG CGCACCAGCA GGAACTGGCG CGGTCCCTGG AGCTGATCGA GGAGAAGGAG 
GGAGAGCTGG ATGAGCTGCG GCTGAAGAAC GTCTCGCGGG GTGAGGAGCT GAGGGACCTG 
CAGAAGACAG TCAGCAAGCT GAAAGGGGAG CTCGCCTCGG TGGAAGCAGT GAAGGAGCGG 
GCGTCCAAGA TGGACAGCGA GCTGCAGGGC TTCCTGGAGG CCGCCCGGAG CCGGGATGCT 
GAGATGGACA GCATCAAGGC AGTGTACGCC AAGGAGGCGT CCCTCAAAAA CCTGGAGGAG 
AAGATCCGCC ACCGAGAGCA GGAGTCTGGC TCCAGCCAAG ACCTCTACCA GGAGAAGCTG 
AAGGAGGCCC AGATGCTGAG CGTGGAAGTG GAGCGGCTGG AGCAGAAGTG CCGGGAGCAG 
CAGGACACCA TCGCTGGGCT GGAGAAAGCC GTGGCTGAAG GATCCCAGCA GCAACAGGCG 
GAGCTGGAGG CATCGCAGAG GGAAGCAGTG CGGCACCGGG AGACGGCAGC GGAGCTGCAG 
AGGCTGCTGG ATGCCTCGCG CTCAGCCCAG GCCCTGCAGG ATGGCACCGT GGAGAGCCTG 
AGGAAGGAGC TGCAGGACAA GAGCAAGGAG CTGGCCCAGA GCAAAACGGC TGTGGCTGCT 
GCCGAGAAGG AGGTGGCATC CCTGCGTGCT GCGGCGCAGG AGAAGGGCCA CCTGGAGGAG 
GGCTGGAAGG AGCAGCTGTC GCAATGCATC CAGGAGCTGG AGAGGAAGAA CAGCCTGCTG 
GGCAGCCTGG AGCACGAGGT GTCCATCCTG CACCGGCAGC TCACCGAGAA GGAGGGCGAG 
AGCAAGGAGC TGAAACGCCT GATCATGGCC GAGTCGGAGA AGAGCAAGAA GCTGGAGGAG 
AGGCTGCGGG TGCTGCAGAC CGAGATGGCC ACGGCCGCAT CGCGGGCGGC CGAACGCTGC 
TCGCTGATGA AGGTGGAGGT GCAACGGTGC CAGGAGGAGA TGGAGAAGCA ACGGATGACC 
ATCGAGGCAC TGAAGAGGGA CCGGCACTGC CAGAGCGAGC GCGAGGAGGA GCTGCGGCAG 
GAGGTGAAGG TCTGCCAGGA CAAGTTCTTC CAGAAGGAGC AGCTCCTCTC CTCCCTGCAG 
CAGGAGCTGG GCAGCGCCCA GGCGTTGGCC GGAGAGGTGG TGCCGCTGAA ACACCTGTGC 
CAGCAGCTGC AAGCTGAGCG TGCCTCGCTG GAGAGCAAGC ACCGCGAGGA CCTGGAGCAA 
AGGGCAAAGG CCACCACTGC GCTGCAGGCG GAGCTGGCCC GGGCCAGGGT GGAGGTGGCC 
GAGCTGCCGG CCCTGCGTGA CAGAGCAGCT GAGCAGGAGC GTGCGCTGCA GAGGCTGCAG 
AAGGAGACAG CGAGCTCCAG CGAGAGGCTG GCAGCCCTGC AAGCAGCCAA CAGCCGGCTG 
GCTGAGGAGA ACCGTGCGCT GAGTGAGAGC GTGAGCCGCG GGCAGCAGCG CCTGGATGCT 
GAGCTGGGGC AGGCAAGGGA GAAGCACACA CGGGAGCTGG AGTGCGTGAG GCTGGAGGCG 
GAGAAGGCGA TGGCCAGCAG CAGGCAGGAG GCCGAGGAGG CGGCCAGGAA GCTGGAGGCG 
ATGAGCAATA AGTATGAAAA CACCAAGGTA AAGGTGCTGG AGGAGAGGCA GAAGTTCCAG 
GAGGAGAGGC AGAAGCTGAT GGCGCAGGTG GAGCAGCTAG AGGTATTTCA GAAAGAACAA 
GCAAAGCAGG TGGAGGAGCT GAATAAGAAG CTGGTGCAGC ACGAGAAGGC CACGCGGAGC 
CAGCAACAGA GAGTTAAGGT GCTGGAAGGA GAGCTGCAGA GCGAGGCCAC GCGCCAGCAG 
GAGAGGGTGG CAGAGCTGGA GGAGCAGCTG GCACAGAAGG AGCAGGCAGC TGAGCACTAC 
AAAGCCCAGA TGGAAAAGGC GAAAACTCAC TACGACGCAA AGAAGCAGCA GAACCAGGAC 
CTGGCAGAGA AGCTGAAGGC CATGGAGCAG CTGCAGAAGG AGAACGCGGA GCTGAGGACG 
GAGTCGGAGC GCTTGGCCAA GGAGCTGCAG CAGAGCGTGC TGCAGGCCAA GGAGGCCGAG 
CTGAGCTGCA GGGAGCTGAG CGGGCAGGTC CGCAGTCTGG AGACACAGCT GGAATTCGCC 
GACCAGCAGC TTCGCGAGCT CGGCAAGTTC CCAGCCCCCA AGGCCACGCT GAAGGAACCG 
GAGAGCTTCC GCCAGAACCC CTCCGACCTC AGCACTGACA GCCTGGACCT CAGCCTGGAC 
GAGGGGCAGC CCCTCAACTC CACCAGGAAA GCCACCCGCT CGCACTCGGA CGGCTCAGCC 
GCTCCGGGTG GCACAGAGCC ACGCGCATCC CAGCGGCTGC CAAGGAAGGT GGAGTCATTG 
GAGAGCCTCT ACTTCACACC CATCGCCAGC CGCACGCAGC CCAAGCTGGA GAGCAGCGCC 
GGTTCTCTGG CCGACGTCTC GCTGGAGTCG GGCTGCAGGA CGCGCTCGGG GCGCCGCCGC 
ACCACACAGA TCATCAACAT CACCATGACC AAGAAAGAGA CCACCACGGA GGAGCCGGGC 
GGCGCGGACG CGTCCTTCAG CAGCATCCTC TCCGAGCAGC CCCAGAAAGC CGCCCCGGCG 
CGGGCCCGGC TGCGCTCTGC TCGCTCCTTG GCCAGCTTTC CCTCGCAGGA CTCCCTGAGC 
AAGCTGGACA CCTCCTCCCC TCAGGAGCCA TCAGGCCACG CTGCGCTGCT CAGCCTCCCC 
GGCTACCGGC CGGCCACGCG CAGCTCGCTG CGGCGCTCGC AGGCGGGCAG CAGCTCCAGC 
TTAGGTCGCA GCAGCATCTA CCTGGGGACG TGCCAGGACG AACCGGAGCA GCTGGACGAT 
TGGAACCGCA TCGCCGAGCT GCAGCAGCGC AACCGCGCCT GCCCGCCGCA CCTCAAGACC 
TGCTACCCCC TGGAGAGCCG GCCTTCCGAC GTGCTGGGCA CCATCACGGA CGAGGAGATG 
AAGACGGGCG ACCCGACGGA GACGCTGCGG CGCGCCAGCA TGCAGCCCTC GCAGATTGCC 
GAGGGGGCGG CCGCCCGGCG CGGCATGCTG GGAGGGGGGT GGGCCCACGG GGGCATCACC 
ACCCGGCAGC AGCGCAAACG GCTCTCGGAT GAATCCCACC AAGGCCCCGA CACCCCCGAG 
TCCAAGAAGC CTGTCAGCTG CTTCCCACGC CCCCAGACCC CCCGCGAGCG GCGCAGCTCC 
CACCTCGGCC GGCGGAGCGA GCAGCAGGCG CCCAGCAAGC AGCCCGAACG GCGCCAGTCC 
ATGGCCTTCA GCATCCTCAA CACCCCCAAG AAGCTGGGGA ACAGCCTCCT GCGCCGGGCT 
GCCAACAGGA AAACCACCCC CAAGAACTCC CCCCGTGGCA CCGCGCGCCG CTCGCCCCGC 
ATCGCCTCCA CCAAATCCCC CAAGGGCAAG GCCGGCCGCA GAGCGCTGAA GGACACCAAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells.
The Journal of cell biology193(1)141-54(2011 Apr)
Peyre E,Jaouen F,Saadaoui M,Haren L,Merdes A,Durbec P,Morin X

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NUMA1 cDNA ORF clone, Gallus gallus(chicken)

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