PLCL1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCL1
Entrez Gene ID	424060
Full Name	phospholipase C like 1
General protein information	Preferred Names phospholipase C like 1 Names inactive phospholipase C-like protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_421916.6	XP_421916.3	inactive phospholipase C-like protein 1
XM_421916.5	XP_421916.3	inactive phospholipase C-like protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLCL1	XP_001169560.1
Macaca mulatta (Rhesus monkey)	PLCL1	XP_001089483.1
Canis lupus familiaris (dog)	PLCL1	XP_536020.4
Rattus norvegicus (Norway rat)	Plcl1	NP_445908.1
Bos taurus (cattle)	PLCL1	NP_001178208.1
Xenopus tropicalis (tropical clawed frog)	plcl1	XP_002937769.2
Gallus gallus (chicken)	PLCL1	XP_421916.3
Mus musculus (house mouse)	Plcl1	NP_001108135.1
Homo sapiens (human)	PLCL1	NP_006217.3

Gene Ontology

The following PLCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa152040	XM_421916.6 Latest version!	Gallus gallus phospholipase C like 1 (PLCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa38365	XM_421916.5 Latest version!	Gallus gallus phospholipase C-like 1 (PLCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCL1 ( XM_421916.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_421916.3 Gallus gallus phospholipase C like 1 (PLCL1), mRNA.

Price & Availability↑

CloneID	OGa152040
Clone ID Related Accession (Same CDS sequence)	XM_421916.6
Accession Version	XM_421916.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	inactive phospholipase C-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_421916.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG CTGCGGGCGG CAGGGAGCGT CCGGCCGGCC GGGCCCCTCC CGACGCCACC 
GCCCGCGCCG AGGAGGAGCA GCCCGAGGCG GCGGGCAGGA GGAGGGAGCG CCGCAGCGGC 
GTTTTGGTCG TCGGTGCCGG AGAGCGCTTC CCCGGAGGCA GCCTGGGGCC GGCGGCTGAG 
CCCGACGCCT CTCTCCTGGA GGCGGCCAAG GCCACCCCGC GCCGGAGCAG CATCATCAAG 
GATCCTTCAA ACCAGAAATG TGGAAGAAAG AAAACGGTGT CCTTCAGCAG CATGCCATCG 
GAGAAGAAAA TAAGCAGCGC TAGTGACTGT ATCAGCTTTA TGCAGGCTGG GTGTGAACTG 
AAGAAAGTTC GTCCAAATTC CCGGATTTAT AACCGTTTTT TTACTTTGGA TCCCGACCTG 
CAGGCTCTTC GCTGGGAGCC TTCCAAAAAG GACCTTGAGA AAGCCAAGCT CGATATTTCT 
GCTATAAAAG AAATAAGACT AGGGAAGAAC ACAGAAACGT TCAGGAATAA TGGGCTTGCA 
GACCAGATTT CTGAGGACTG TGCGCTGTCG GTAATTCACG GTGAGAACTA TGAGTCCCTC 
GACCTGGTTG CCAATTCAGC TGACGTGGCC AATATTTGGG TATCGGGATT AAGGTACTTG 
GTTTCTCGTA GCAAACAACC CCTGGATTTG ATGGAAAGCA GCCATAATAC CCCACGGTTT 
GCCTGGCTGA AAACTGTCTT TGAAGCAGCA GACGTTGATG GCAATGGCAT AATGTTAGAA 
GATACATCTG TGGAACTAAT AAAGAAGCTT AACCCCACCT TAAAGGAATC AAAAATTAGA 
TTAAAATTCA AGGAAATCCA GAAGAGCAAA GAGAAACTGA CAACACGAGT AACAGAAGAG 
GAATTTTGTG AAGCGTTCTG CGAACTTTGC ACAAGACCTG AAGTTTATTT CTTACTTGTG 
CAGATATCTA AAAACAAAGA GTATCTAGAT GCCAATGACC TCATGCTGTT TTTAGAGGCT 
GAACAAGGAG TGACCCACAT AACAGAAGAA ATGTGTCTAG ATATTATACG CAGGTATGAG 
CTTTCTCAAG AAGGACGTTT GAAAGGTTTT CTTGCAATTG ATGGATTTAC TCAATATTTG 
CTGTCCCCAG AATGTGATAT TTTTGATCCT GAGCACAAAA AGATTGTCCA AGACATGACA 
CAGCCCTTGT CGCATTACTA CATCAATGCA TCCCACAATA CGTATCTAAT TGAAGACCAA 
CTCAGAGGGC CGGCTGATAT CAATGGTTAC GTAAGGGCTT TAAAAATGAG TTGTCGGAGT 
ATTGAACTAG ATGTTTGTGA TGGCCCAGAT AATGAGCCCA TTATTTGTAA CCGTAACAAC 
ATGACATCGC CACTTTCATT TCAGAATGTC ATTGAGGTGA TAAACAAATT GGCTTTCTTT 
GCATCAGAAT ACCCCCTTAT TCTCTGCTTG GGGAACCACT GCTCCGTACA ACAGCAGAAG 
GTTGTGGTAC AACACATGAA AAGGATCTTT GGAAACAAGC TCTACACGGA AGCACCTTTA 
TCTTCGGAAG CCTACCTCCC ATCACCTGAG AAACTGAAAA TGAAGATAAT TGTAAAGGGG 
AAGAAGCTAC CCTGCGACCA GGATATACTA GAAGGGGAAG TCACAGATGA AGATGAAGAG 
GCTGAAATAT CCCGGAGACT GTCAGAAGAC TTCTCAAGGG AACCAAAGCT GATCTGGCTC 
TGTAAGGAGT TGTCTGACTT GGTGTCCCTA TGCAAATCTG TCCAATACAA AGACTTTGAG 
ACATCCATGA AAGCTCAAAA TTATTGGGAA ATCTGTTCCT TTAGTGAAGC AGAAGCCAGT 
CGAATCGCAA ATGAATACCC TGAAGATTTT GTCAACTACA ACAAAAAGTT TCTTTCCAGG 
GTGTACCCGA GTGCTATGAG GATAGACTCC AGTAACTTAA ATCCTCAAGA TTTTTGGAAT 
TGTGGTTGTC AGATAGTGGC AATGAACTAT CAGACTCCAG GGCCCATGAT GGACCTGCAT 
ACTGGCTGGT TTCTACAGAA TGGTGGATGC GGGTATGTTC TCAGGCCTTC GGTAATGCGT 
GAAGAAGTGT CTTATTTCAG TGCAAATACA AAGGGCATTG TTCCGGGGGT CTCTCCCCAA 
GTTCTACATG TCAAAATAAT CAGTGGACAG AACTTTCCAA AGCCCAAAGG TGCGTGTGCA 
AAAGGGGATG TCATAGATCC CTATGTTTGT ATAGAAATAC ATGGGATTCC TGCAGATTGC 
ACTGAACAAA GAACTAAAAC TGTTCAGCAA AACAGTGATA ACCCAATTTT TGATGAGAGC 
TTTGAGTTCC AGATCAATCT ACCAGAGTTG GCCATGATCC GTTTTGTTGT TTTGGATGAT 
GACTACATTG GGGATGAGTT CATCGGCCAG TACACCATCC CACTGGAGTG CCTGCAGCCT 
GGATACAGGC ACATACCGCT GCGGTCTTTT GTTGGTGATA TCATGGAGCA CGTTACGCTG 
TTTGTTCATA TTGCAATAAC CAATCGAAGT GGAGGTGGAA AGGCCCAAAA GCGCAGCCTC 
TCTGTGAGGA TAGGAAAGAA AGCAAGGGAG TACACCATGC TGAGGAACAC TGGGCTCAAG 
ACTATTGACG ACATCTTTAA GTTGGCTGTG CATCCACTAC GAGAGGCCAC CGACATGAGA 
GAGAACATGC AGAACGCCAT GGTGTCCGTT AAAGAGCTGT GTGGGCTGCC ACCCATCGCA 
AGCCTGAAGC AGTGCATCCT GACCCTGTCC TCGCGGCTCG TCAACAGTGA GAATGCACCC 
TCTGTTACCC TCTGTATGAA GGACGCGTTT CCTTACCTAG AGCCTCTGGG GACCATGCCT 
GACGTGCAGA AAAAGGTTCT GGCTGCATAT GACCTGATGA TTCAAGAGAG CAGGGTTCTT 
ATCGAGACGG CAGACATCAC TCACGACAAG ATCGTTCAGT GTCAGAAAGC AGGAATGGAA 
TTCCATGAAG AGCTTCATAA CCTCGGGACA AAGGAAGGTT TGAAAGGAAG AAAATTAAGC 
AAAGCCATTG AAAGCTTTGC GTGGAATATC ACAGTGCTGA AGGGCCAAGG AGACCTCCTG 
AAGAATGCCA AGAATGAAGC CCTGGAAAAC ATGAAACAGA TCCAGCTGGC GTGCCTATCC 
TGCGGACTGA GCAAAACTGG AAGTGGGCAT GCAGATGCAA AGTCAAAACG GTGCCTAGAA 
GCGATACAGG AGAAGGATAC AGGAGATGAA AACGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_421916.3
CDS	134..3418
Translation	MAEAAGGRERPAGRAPPDATARAEEEQPEAAGRRRERRSGVLVVGAGERFPGGSLGPAAEPDASLLEAAKATPRRSSIIKDPSNQKCGRKKTVSFSSMPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDPDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQISEDCALSVIHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDLMESSHNTPRFAWLKTVFEAADVDGNGIMLEDTSVELIKKLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEEMCLDIIRRYELSQEGRLKGFLAIDGFTQYLLSPECDIFDPEHKKIVQDMTQPLSHYYINASHNTYLIEDQLRGPADINGYVRALKMSCRSIELDVCDGPDNEPIICNRNNMTSPLSFQNVIEVINKLAFFASEYPLILCLGNHCSVQQQKVVVQHMKRIFGNKLYTEAPLSSEAYLPSPEKLKMKIIVKGKKLPCDQDILEGEVTDEDEEAEISRRLSEDFSREPKLIWLCKELSDLVSLCKSVQYKDFETSMKAQNYWEICSFSEAEASRIANEYPEDFVNYNKKFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNYQTPGPMMDLHTGWFLQNGGCGYVLRPSVMREEVSYFSANTKGIVPGVSPQVLHVKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCTEQRTKTVQQNSDNPIFDESFEFQINLPELAMIRFVVLDDDYIGDEFIGQYTIPLECLQPGYRHIPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRIGKKAREYTMLRNTGLKTIDDIFKLAVHPLREATDMRENMQNAMVSVKELCGLPPIASLKQCILTLSSRLVNSENAPSVTLCMKDAFPYLEPLGTMPDVQKKVLAAYDLMIQESRVLIETADITHDKIVQCQKAGMEFHEELHNLGTKEGLKGRKLSKAIESFAWNITVLKGQGDLLKNAKNEALENMKQIQLACLSCGLSKTGSGHADAKSKRCLEAIQEKDTGDENGRL

Target ORF information:

RefSeq Version	XM_421916.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C like 1 (PLCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_421916.6

ORF Insert Sequence:

ATGGCAGAGG CTGCGGGCGG CAGGGAGCGT CCGGCCGGCC GGGCCCCTCC CGACGCCACC 
GCCCGCGCCG AGGAGGAGCA GCCCGAGGCG GCGGGCAGGA GGAGGGAGCG CCGCAGCGGC 
GTTTTGGTCG TCGGTGCCGG AGAGCGCTTC CCCGGAGGCA GCCTGGGGCC GGCGGCTGAG 
CCCGACGCCT CTCTCCTGGA GGCGGCCAAG GCCACCCCGC GCCGGAGCAG CATCATCAAG 
GATCCTTCAA ACCAGAAATG TGGAAGAAAG AAAACGGTGT CCTTCAGCAG CATGCCATCG 
GAGAAGAAAA TAAGCAGCGC TAGTGACTGT ATCAGCTTTA TGCAGGCTGG GTGTGAACTG 
AAGAAAGTTC GTCCAAATTC CCGGATTTAT AACCGTTTTT TTACTTTGGA TCCCGACCTG 
CAGGCTCTTC GCTGGGAGCC TTCCAAAAAG GACCTTGAGA AAGCCAAGCT CGATATTTCT 
GCTATAAAAG AAATAAGACT AGGGAAGAAC ACAGAAACGT TCAGGAATAA TGGGCTTGCA 
GACCAGATTT CTGAGGACTG TGCGCTGTCG GTAATTCACG GTGAGAACTA TGAGTCCCTC 
GACCTGGTTG CCAATTCAGC TGACGTGGCC AATATTTGGG TATCGGGATT AAGGTACTTG 
GTTTCTCGTA GCAAACAACC CCTGGATTTG ATGGAAAGCA GCCATAATAC CCCACGGTTT 
GCCTGGCTGA AAACTGTCTT TGAAGCAGCA GACGTTGATG GCAATGGCAT AATGTTAGAA 
GATACATCTG TGGAACTAAT AAAGAAGCTT AACCCCACCT TAAAGGAATC AAAAATTAGA 
TTAAAATTCA AGGAAATCCA GAAGAGCAAA GAGAAACTGA CAACACGAGT AACAGAAGAG 
GAATTTTGTG AAGCGTTCTG CGAACTTTGC ACAAGACCTG AAGTTTATTT CTTACTTGTG 
CAGATATCTA AAAACAAAGA GTATCTAGAT GCCAATGACC TCATGCTGTT TTTAGAGGCT 
GAACAAGGAG TGACCCACAT AACAGAAGAA ATGTGTCTAG ATATTATACG CAGGTATGAG 
CTTTCTCAAG AAGGACGTTT GAAAGGTTTT CTTGCAATTG ATGGATTTAC TCAATATTTG 
CTGTCCCCAG AATGTGATAT TTTTGATCCT GAGCACAAAA AGATTGTCCA AGACATGACA 
CAGCCCTTGT CGCATTACTA CATCAATGCA TCCCACAATA CGTATCTAAT TGAAGACCAA 
CTCAGAGGGC CGGCTGATAT CAATGGTTAC GTAAGGGCTT TAAAAATGAG TTGTCGGAGT 
ATTGAACTAG ATGTTTGTGA TGGCCCAGAT AATGAGCCCA TTATTTGTAA CCGTAACAAC 
ATGACATCGC CACTTTCATT TCAGAATGTC ATTGAGGTGA TAAACAAATT GGCTTTCTTT 
GCATCAGAAT ACCCCCTTAT TCTCTGCTTG GGGAACCACT GCTCCGTACA ACAGCAGAAG 
GTTGTGGTAC AACACATGAA AAGGATCTTT GGAAACAAGC TCTACACGGA AGCACCTTTA 
TCTTCGGAAG CCTACCTCCC ATCACCTGAG AAACTGAAAA TGAAGATAAT TGTAAAGGGG 
AAGAAGCTAC CCTGCGACCA GGATATACTA GAAGGGGAAG TCACAGATGA AGATGAAGAG 
GCTGAAATAT CCCGGAGACT GTCAGAAGAC TTCTCAAGGG AACCAAAGCT GATCTGGCTC 
TGTAAGGAGT TGTCTGACTT GGTGTCCCTA TGCAAATCTG TCCAATACAA AGACTTTGAG 
ACATCCATGA AAGCTCAAAA TTATTGGGAA ATCTGTTCCT TTAGTGAAGC AGAAGCCAGT 
CGAATCGCAA ATGAATACCC TGAAGATTTT GTCAACTACA ACAAAAAGTT TCTTTCCAGG 
GTGTACCCGA GTGCTATGAG GATAGACTCC AGTAACTTAA ATCCTCAAGA TTTTTGGAAT 
TGTGGTTGTC AGATAGTGGC AATGAACTAT CAGACTCCAG GGCCCATGAT GGACCTGCAT 
ACTGGCTGGT TTCTACAGAA TGGTGGATGC GGGTATGTTC TCAGGCCTTC GGTAATGCGT 
GAAGAAGTGT CTTATTTCAG TGCAAATACA AAGGGCATTG TTCCGGGGGT CTCTCCCCAA 
GTTCTACATG TCAAAATAAT CAGTGGACAG AACTTTCCAA AGCCCAAAGG TGCGTGTGCA 
AAAGGGGATG TCATAGATCC CTATGTTTGT ATAGAAATAC ATGGGATTCC TGCAGATTGC 
ACTGAACAAA GAACTAAAAC TGTTCAGCAA AACAGTGATA ACCCAATTTT TGATGAGAGC 
TTTGAGTTCC AGATCAATCT ACCAGAGTTG GCCATGATCC GTTTTGTTGT TTTGGATGAT 
GACTACATTG GGGATGAGTT CATCGGCCAG TACACCATCC CACTGGAGTG CCTGCAGCCT 
GGATACAGGC ACATACCGCT GCGGTCTTTT GTTGGTGATA TCATGGAGCA CGTTACGCTG 
TTTGTTCATA TTGCAATAAC CAATCGAAGT GGAGGTGGAA AGGCCCAAAA GCGCAGCCTC 
TCTGTGAGGA TAGGAAAGAA AGCAAGGGAG TACACCATGC TGAGGAACAC TGGGCTCAAG 
ACTATTGACG ACATCTTTAA GTTGGCTGTG CATCCACTAC GAGAGGCCAC CGACATGAGA 
GAGAACATGC AGAACGCCAT GGTGTCCGTT AAAGAGCTGT GTGGGCTGCC ACCCATCGCA 
AGCCTGAAGC AGTGCATCCT GACCCTGTCC TCGCGGCTCG TCAACAGTGA GAATGCACCC 
TCTGTTACCC TCTGTATGAA GGACGCGTTT CCTTACCTAG AGCCTCTGGG GACCATGCCT 
GACGTGCAGA AAAAGGTTCT GGCTGCATAT GACCTGATGA TTCAAGAGAG CAGGGTTCTT 
ATCGAGACGG CAGACATCAC TCACGACAAG ATCGTTCAGT GTCAGAAAGC AGGAATGGAA 
TTCCATGAAG AGCTTCATAA CCTCGGGACA AAGGAAGGTT TGAAAGGAAG AAAATTAAGC 
AAAGCCATTG AAAGCTTTGC GTGGAATATC ACAGTGCTGA AGGGCCAAGG AGACCTCCTG 
AAGAATGCCA AGAATGAAGC CCTGGAAAAC ATGAAACAGA TCCAGCTGGC GTGCCTATCC 
TGCGGACTGA GCAAAACTGG AAGTGGGCAT GCAGATGCAA AGTCAAAACG GTGCCTAGAA 
GCGATACAGG AGAAGGATAC AGGAGATGAA AACGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_421916.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_421916.3 Gallus gallus phospholipase C-like 1 (PLCL1), mRNA.

Price & Availability↑

CloneID	OGa38365
Clone ID Related Accession (Same CDS sequence)	XM_421916.5
Accession Version	XM_421916.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	inactive phospholipase C-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_421916.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGG CTGCGGGCGG CAGGGAGCGT CCGGCCGGCC GGGCCCCTCC CGACGCCACC 
GCCCGCGCCG AGGAGGAGCA GCCCGAGGCG GCGGGCAGGA GGAGGGAGCG CCGCAGCGGC 
GTTTTGGTCG TCGGTGCCGG AGAGCGCTTC CCCGGAGGCA GCCTGGGGCC GGCGGCTGAG 
CCCGACGCCT CTCTCCTGGA GGCGGCCAAG GCCACCCCGC GCCGGAGCAG CATCATCAAG 
GATCCTTCAA ACCAGAAATG TGGAAGAAAG AAAACGGTGT CCTTCAGCAG CATGCCATCA 
GAGAAGAAAA TAAGCAGCGC TAGTGACTGT ATCAGCTTTA TGCAGGCTGG GTGTGAACTG 
AAGAAAGTTC GTCCAAATTC CCGGATTTAT AACCGTTTTT TTACTTTGGA TCCCGACCTG 
CAGGCTCTTC GTTGGGAGCC TTCCAAAAAG GACCTTGAGA AAGCCAAGCT CGATATTTCT 
GCTATAAAAG AAATAAGACT AGGGAAGAAC ACAGAAACGT TCAGGAATAA TGGACTTGCA 
GACCAGATTT CTGAGGACTG TGCGCTGTCG GTAATTCACG GTGAGAACTA TGAGTCCCTC 
GACCTGGTTG CCAATTCAGC TGACGTGGCC AATATTTGGG TATCGGGATT AAGGTACTTG 
GTTTCTCGTA GCAAACAACC CCTGGATTTG ATGGAAAGCA GCCATAATAC CCCACGGTTT 
GCCTGGCTGA AAACTGTCTT TGAAGCAGCA GACGTTGATG GCAATGGCAT AATGTTAGAA 
GATACATCTG TGGAACTAAT AAAGAAGCTT AACCCCACCT TAAAGGAATC AAAAATTAGA 
TTAAAATTCA AGGAAATCCA GAAGAGCAAA GAGAAACTGA CAACACGAGT AACAGAAGAG 
GAATTTTGTG AAGCGTTCTG CGAACTTTGC ACAAGACCTG AAGTTTATTT CTTACTTGTG 
CAGATATCTA AAAACAAAGA GTATCTAGAT GCCAATGACC TCATGCTGTT TTTAGAGGCT 
GAACAAGGAG TGACCCACAT AACAGAAGAA ATGTGTCTAG ATATTATACG CAGGTATGAG 
CTTTCTCAAG AAGGACGTTT GAAAGGTTTT CTTGCAATTG ATGGATTTAC TCAATATTTG 
CTGTCCCCAG AATGTGATAT TTTTGATCCT GAGCACAAAA AGATTGTCCA AGACATGACA 
CAGCCCTTGT CGCATTACTA CATCAATGCA TCCCACAATA CGTATCTAAT TGAAGACCAA 
CTCAGAGGGC CGGCTGATAT CAATGGTTAC GTAAGGGCTT TAAAAATGAG TTGTCGGAGT 
ATTGAACTAG ATGTTTGTGA TGGCCCAGAT AATGAGCCCA TTATTTGTAA CCGTAACAAC 
ATGACATCGC CACTTTCATT TCAGAATGTC ATTGAGGTGA TAAACAAATT GGCTTTCTTT 
GCATCAGAAT ACCCCCTTAT TCTCTGCTTG GGGAACCACT GCTCCGTACA ACAGCAGAAG 
GTTGTGGTAC AACACATGAA AAGGATCTTT GGAAACAAGC TCTACACGGA AGCACCTTTA 
TCTTCGGAAG CCTACCTCCC ATCACCTGAG AAACTGAAAA TGAAGATAAT TGTAAAGGGG 
AAGAAGCTAC CCTGCGACCA GGATATACTA GAAGGGGAAG TCACAGATGA AGATGAAGAG 
GCTGAAATAT CCCGGAGACT GTCAGAAGAC TTCTCAAGGG AACCAAAGCT GATCTGGCTC 
TGCAAGGAGT TGTCTGACTT GGTGTCCCTA TGCAAATCTG TCCAATACAA AGACTTTGAG 
ACATCCATGA AAGCTCAAAA TTATTGGGAA ATCTGTTCCT TTAGTGAAGC AGAAGCCAGT 
CGAATCGCAA ATGAATACCC TGAAGATTTT GTCAACTACA ACAAAAAGTT TCTTTCCAGG 
GTGTACCCGA GTGCTATGAG GATAGACTCC AGTAACTTAA ATCCTCAAGA TTTTTGGAAT 
TGTGGTTGTC AGATAGTGGC AATGAACTAT CAGACTCCAG GGCCCATGAT GGACCTGCAT 
ACTGGCTGGT TTCTACAGAA TGGTGGATGC GGGTATGTTC TCAGGCCTTC GGTAATGCGT 
GAAGAAGTGT CTTATTTCAG TGCAAATACA AAGGGCATTG TTCCGGGGGT CTCTCCCCAA 
GTTCTACATG TCAAAATAAT CAGTGGACAG AACTTTCCAA AGCCCAAAGG TGCGTGTGCA 
AAAGGGGATG TCATAGATCC CTATGTTTGT ATAGAAATAC ATGGGATTCC TGCAGATTGC 
ACTGAACAAA GAACTAAAAC TGTTCAGCAA AACAGTGATA ACCCAATTTT TGATGAGAGC 
TTTGAGTTCC AGATCAATCT ACCAGAGTTG GCCATGATCC GTTTTGTTGT TTTGGATGAT 
GACTACATTG GGGATGAGTT CATCGGCCAG TACACCATCC CACTGGAGTG CCTGCAGCCT 
GGATACAGGC ACATACCGCT GCGGTCTTTT GTTGGTGATA TCATGGAGCA CGTTACGCTG 
TTTGTTCATA TTGCAATAAC CAATCGAAGT GGAGGTGGAA AGGCCCAAAA GCGCAGCCTC 
TCTGTGAGGA TAGGAAAGAA AGCAAGGGAG TACACCATGC TGAGGAACAC TGGGCTCAAG 
ACTATTGACG ACATCTTTAA GTTGGCTGTG CATCCTCTAC GAGAGGCCAC CGACATGAGA 
GAGAACATGC AGAACGCCAT GGTGTCCGTT AAAGAGCTGT GTGGGCTGCC ACCCATCGCA 
AGCCTGAAGC AGTGCATCCT GACCCTGTCC TCGCGGCTCG TCAACAGTGA GAATGCACCC 
TCTGTTACCC TCTGTATGAA GGACGCGTTT CCTTACCTAG AGCCTCTGGG GACCATGCCT 
GACGTGCAGA AAAAGGTTCT GGCTGCATAT GACCTGATGA TTCAAGAGAG CAGGGTTCTT 
ATCGAGACGG CAGACATCAC TCACGACAAG ATCGTTCAGT GTCAGAAAGC AGGAATGGAA 
TTCCATGAAG AGCTTCATAA CCTCGGGACA AAGGAAGGTT TGAAAGGAAG AAAATTAAGC 
AAAGCCATTG AAAGCTTTGC GTGGAATATC ACAGTGCTGA AGGGCCAAGG AGACCTCCTG 
AAGAATGCCA AGAATGAAGC CCTGGAAAAC ATGAAACAGA TCCAGCTGGC GTGCCTATCC 
TGCGGACTGA GCAAAACTGG AAGTGGGCAT GCAGATGCAA AGTCAAAACG GTGCCTAGAA 
GCGATACAGG AGAAGGATAC AGGAGATGAA AACGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_421916.3
CDS	266..3550
Translation	MAEAAGGRERPAGRAPPDATARAEEEQPEAAGRRRERRSGVLVVGAGERFPGGSLGPAAEPDASLLEAAKATPRRSSIIKDPSNQKCGRKKTVSFSSMPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDPDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQISEDCALSVIHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDLMESSHNTPRFAWLKTVFEAADVDGNGIMLEDTSVELIKKLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEEMCLDIIRRYELSQEGRLKGFLAIDGFTQYLLSPECDIFDPEHKKIVQDMTQPLSHYYINASHNTYLIEDQLRGPADINGYVRALKMSCRSIELDVCDGPDNEPIICNRNNMTSPLSFQNVIEVINKLAFFASEYPLILCLGNHCSVQQQKVVVQHMKRIFGNKLYTEAPLSSEAYLPSPEKLKMKIIVKGKKLPCDQDILEGEVTDEDEEAEISRRLSEDFSREPKLIWLCKELSDLVSLCKSVQYKDFETSMKAQNYWEICSFSEAEASRIANEYPEDFVNYNKKFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNYQTPGPMMDLHTGWFLQNGGCGYVLRPSVMREEVSYFSANTKGIVPGVSPQVLHVKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCTEQRTKTVQQNSDNPIFDESFEFQINLPELAMIRFVVLDDDYIGDEFIGQYTIPLECLQPGYRHIPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRIGKKAREYTMLRNTGLKTIDDIFKLAVHPLREATDMRENMQNAMVSVKELCGLPPIASLKQCILTLSSRLVNSENAPSVTLCMKDAFPYLEPLGTMPDVQKKVLAAYDLMIQESRVLIETADITHDKIVQCQKAGMEFHEELHNLGTKEGLKGRKLSKAIESFAWNITVLKGQGDLLKNAKNEALENMKQIQLACLSCGLSKTGSGHADAKSKRCLEAIQEKDTGDENGRL

Target ORF information:

RefSeq Version	XM_421916.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phospholipase C-like 1 (PLCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_421916.5

ORF Insert Sequence:

ATGGCAGAGG CTGCGGGCGG CAGGGAGCGT CCGGCCGGCC GGGCCCCTCC CGACGCCACC 
GCCCGCGCCG AGGAGGAGCA GCCCGAGGCG GCGGGCAGGA GGAGGGAGCG CCGCAGCGGC 
GTTTTGGTCG TCGGTGCCGG AGAGCGCTTC CCCGGAGGCA GCCTGGGGCC GGCGGCTGAG 
CCCGACGCCT CTCTCCTGGA GGCGGCCAAG GCCACCCCGC GCCGGAGCAG CATCATCAAG 
GATCCTTCAA ACCAGAAATG TGGAAGAAAG AAAACGGTGT CCTTCAGCAG CATGCCATCA 
GAGAAGAAAA TAAGCAGCGC TAGTGACTGT ATCAGCTTTA TGCAGGCTGG GTGTGAACTG 
AAGAAAGTTC GTCCAAATTC CCGGATTTAT AACCGTTTTT TTACTTTGGA TCCCGACCTG 
CAGGCTCTTC GTTGGGAGCC TTCCAAAAAG GACCTTGAGA AAGCCAAGCT CGATATTTCT 
GCTATAAAAG AAATAAGACT AGGGAAGAAC ACAGAAACGT TCAGGAATAA TGGACTTGCA 
GACCAGATTT CTGAGGACTG TGCGCTGTCG GTAATTCACG GTGAGAACTA TGAGTCCCTC 
GACCTGGTTG CCAATTCAGC TGACGTGGCC AATATTTGGG TATCGGGATT AAGGTACTTG 
GTTTCTCGTA GCAAACAACC CCTGGATTTG ATGGAAAGCA GCCATAATAC CCCACGGTTT 
GCCTGGCTGA AAACTGTCTT TGAAGCAGCA GACGTTGATG GCAATGGCAT AATGTTAGAA 
GATACATCTG TGGAACTAAT AAAGAAGCTT AACCCCACCT TAAAGGAATC AAAAATTAGA 
TTAAAATTCA AGGAAATCCA GAAGAGCAAA GAGAAACTGA CAACACGAGT AACAGAAGAG 
GAATTTTGTG AAGCGTTCTG CGAACTTTGC ACAAGACCTG AAGTTTATTT CTTACTTGTG 
CAGATATCTA AAAACAAAGA GTATCTAGAT GCCAATGACC TCATGCTGTT TTTAGAGGCT 
GAACAAGGAG TGACCCACAT AACAGAAGAA ATGTGTCTAG ATATTATACG CAGGTATGAG 
CTTTCTCAAG AAGGACGTTT GAAAGGTTTT CTTGCAATTG ATGGATTTAC TCAATATTTG 
CTGTCCCCAG AATGTGATAT TTTTGATCCT GAGCACAAAA AGATTGTCCA AGACATGACA 
CAGCCCTTGT CGCATTACTA CATCAATGCA TCCCACAATA CGTATCTAAT TGAAGACCAA 
CTCAGAGGGC CGGCTGATAT CAATGGTTAC GTAAGGGCTT TAAAAATGAG TTGTCGGAGT 
ATTGAACTAG ATGTTTGTGA TGGCCCAGAT AATGAGCCCA TTATTTGTAA CCGTAACAAC 
ATGACATCGC CACTTTCATT TCAGAATGTC ATTGAGGTGA TAAACAAATT GGCTTTCTTT 
GCATCAGAAT ACCCCCTTAT TCTCTGCTTG GGGAACCACT GCTCCGTACA ACAGCAGAAG 
GTTGTGGTAC AACACATGAA AAGGATCTTT GGAAACAAGC TCTACACGGA AGCACCTTTA 
TCTTCGGAAG CCTACCTCCC ATCACCTGAG AAACTGAAAA TGAAGATAAT TGTAAAGGGG 
AAGAAGCTAC CCTGCGACCA GGATATACTA GAAGGGGAAG TCACAGATGA AGATGAAGAG 
GCTGAAATAT CCCGGAGACT GTCAGAAGAC TTCTCAAGGG AACCAAAGCT GATCTGGCTC 
TGCAAGGAGT TGTCTGACTT GGTGTCCCTA TGCAAATCTG TCCAATACAA AGACTTTGAG 
ACATCCATGA AAGCTCAAAA TTATTGGGAA ATCTGTTCCT TTAGTGAAGC AGAAGCCAGT 
CGAATCGCAA ATGAATACCC TGAAGATTTT GTCAACTACA ACAAAAAGTT TCTTTCCAGG 
GTGTACCCGA GTGCTATGAG GATAGACTCC AGTAACTTAA ATCCTCAAGA TTTTTGGAAT 
TGTGGTTGTC AGATAGTGGC AATGAACTAT CAGACTCCAG GGCCCATGAT GGACCTGCAT 
ACTGGCTGGT TTCTACAGAA TGGTGGATGC GGGTATGTTC TCAGGCCTTC GGTAATGCGT 
GAAGAAGTGT CTTATTTCAG TGCAAATACA AAGGGCATTG TTCCGGGGGT CTCTCCCCAA 
GTTCTACATG TCAAAATAAT CAGTGGACAG AACTTTCCAA AGCCCAAAGG TGCGTGTGCA 
AAAGGGGATG TCATAGATCC CTATGTTTGT ATAGAAATAC ATGGGATTCC TGCAGATTGC 
ACTGAACAAA GAACTAAAAC TGTTCAGCAA AACAGTGATA ACCCAATTTT TGATGAGAGC 
TTTGAGTTCC AGATCAATCT ACCAGAGTTG GCCATGATCC GTTTTGTTGT TTTGGATGAT 
GACTACATTG GGGATGAGTT CATCGGCCAG TACACCATCC CACTGGAGTG CCTGCAGCCT 
GGATACAGGC ACATACCGCT GCGGTCTTTT GTTGGTGATA TCATGGAGCA CGTTACGCTG 
TTTGTTCATA TTGCAATAAC CAATCGAAGT GGAGGTGGAA AGGCCCAAAA GCGCAGCCTC 
TCTGTGAGGA TAGGAAAGAA AGCAAGGGAG TACACCATGC TGAGGAACAC TGGGCTCAAG 
ACTATTGACG ACATCTTTAA GTTGGCTGTG CATCCTCTAC GAGAGGCCAC CGACATGAGA 
GAGAACATGC AGAACGCCAT GGTGTCCGTT AAAGAGCTGT GTGGGCTGCC ACCCATCGCA 
AGCCTGAAGC AGTGCATCCT GACCCTGTCC TCGCGGCTCG TCAACAGTGA GAATGCACCC 
TCTGTTACCC TCTGTATGAA GGACGCGTTT CCTTACCTAG AGCCTCTGGG GACCATGCCT 
GACGTGCAGA AAAAGGTTCT GGCTGCATAT GACCTGATGA TTCAAGAGAG CAGGGTTCTT 
ATCGAGACGG CAGACATCAC TCACGACAAG ATCGTTCAGT GTCAGAAAGC AGGAATGGAA 
TTCCATGAAG AGCTTCATAA CCTCGGGACA AAGGAAGGTT TGAAAGGAAG AAAATTAAGC 
AAAGCCATTG AAAGCTTTGC GTGGAATATC ACAGTGCTGA AGGGCCAAGG AGACCTCCTG 
AAGAATGCCA AGAATGAAGC CCTGGAAAAC ATGAAACAGA TCCAGCTGGC GTGCCTATCC 
TGCGGACTGA GCAAAACTGG AAGTGGGCAT GCAGATGCAA AGTCAAAACG GTGCCTAGAA 
GCGATACAGG AGAAGGATAC AGGAGATGAA AACGGGAGGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PLCL1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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