VWDE cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	VWDE
Entrez Gene ID	420587
Full Name	von Willebrand factor D and EGF domains
General protein information	Preferred Names von Willebrand factor D and EGF domains Names von Willebrand factor D and EGF domain-containing protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015281603.1	XP_015137089.1	von Willebrand factor D and EGF domain-containing protein
XM_025147365.1	XP_025003133.1	von Willebrand factor D and EGF domain-containing protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Vwde	NP_001013779.2
Danio rerio (zebrafish)	LOC100006681	XP_001345360.4
Xenopus tropicalis (tropical clawed frog)	vwde	XP_002939057.2
Pan troglodytes (chimpanzee)	VWDE	XP_518974.3
Macaca mulatta (Rhesus monkey)	LOC694004	XP_002803427.1
Canis lupus familiaris (dog)	VWDE	XP_005628620.1
Rattus norvegicus (Norway rat)	Vwde	XP_006236185.1
Gallus gallus (chicken)	VWDE	XP_418688.4
Bos taurus (cattle)	VWDE	XP_003585998.2
Homo sapiens (human)	VWDE	NP_001129396.1

Gene Ontology

The following VWDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VWDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa145978	XM_025147365.1 Latest version!	Gallus gallus von Willebrand factor D and EGF domains (VWDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa31683	XM_015281603.1 Latest version!	Gallus gallus von Willebrand factor D and EGF domains (VWDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VWDE ( XM_025147365.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025003133.1 Gallus gallus von Willebrand factor D and EGF domains (VWDE), mRNA.

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CloneID	OGa145978
Clone ID Related Accession (Same CDS sequence)	XM_025147365.1
Accession Version	XM_025147365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	von Willebrand factor D and EGF domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG GCGCCCGCCT GTGGCTGTGC GCGGCCGTGC TCGGCTGCCT GGCGGCGGCG 
AAGCCCCGCC GGAGAGTTCT TGAATGCCAT CCTGGTGGAC ATCAGATCCT GCAGAGCCCT 
TACCGAAGTG TTGATTTTGA TTCATCCCAC CTCCAGCAGT CAGCTATTCA GGATTTGATA 
TGTGACCATT CCCTAGCACC AGGATGGTAT CGCTTTATGA TTTTTGATAA GCCTGCTGAG 
ATGCCAACCA AGTGTGTGGA GATGAATCAC TGTGGTACTC AAGCCCCTGT CTGGTTGTCT 
CTGAAAGAGT CAGAATCCAT GCCTCGTCCT GGTGAGATCA AGCAGCTCAC AGCCTGTGCA 
ACATGGAAGT TTTTCTTCAG CACTTACAAA GATTGCTGCC TTTTTCAAAT CCCTGTCAGT 
GTGAGGAACT GTGGAGATTT CTTTGTTTAC TTACTACAAC CCACACAAGG ATGCATGGGT 
TACTGTGCAG AAGGTAAGGT GGCTCCTTCA ACATCTCCGT CTGTGTCACC ACCACTACCA 
GCCATTCCTG AGGTTGCAGC AGAGTTGATC AAAGGGAGCA TTTACCTAAG GTGTACCTTT 
GGCATTCCTT TTGCAAACAG CTCAGTTGGA TTCATTGTGA CTTGGTCGAG ACTTTCTCCT 
GAAGGCATCA AAGAAGAACT GAAACATGAG ACAACAGTTC ATACATTCTC ACTCTTAGAG 
TTAGATGGGA TAAATGTCAG ACTTGGAGAA AGAGTCTACT GTAGCAGTTC TGCTTTCTTC 
ATGGAGAAGC CAAGCATACA GAGCAGTGCA GTTGAGAGCA AGGAATTTCT TGCTGGCATT 
AAGATACATC CAGAAGCGTT TGATATATCA GAAGATGGCA AGGAATACAG ACTGTCAATA 
GAAAGCAGAA TCCCTATTCC TTGTCCTGAA TTTAACCAGG TTGAAAGTGA CTGCAGAATA 
TCATTAAGAT TGAATACTGT TGATGAAGGT AAAGAACAAT TAGGTTTAAA CTTGGCTCTT 
TCTTCTTGTC ATGTGGATCT TCTCCAGAAA CCCTGCAGAA ATGGAATCTG TAGTCAAGCT 
ATTATTTATT TCACTGCTGT GACAGACTTT ATGCAGGATG GAGACAGAAT TACCAGAATT 
GCAGTTGAAC CTATATCCAG TGAGAATTTC CTGTGGAATG GCTATGTTCC AGAAAGCACA 
AAGATTACAG TAAAGGATCT ACCAACTGCC TACTGCTACT CTTTTACTGA CCCTCATATA 
ATTACGTTTG ATGGAAGACT CTATGACAAT TTTAAAACAG GAACATTTGT TCTCTACAAG 
AGTACAGCTC GAGATTTTGA AGTTCATGTT CGCCAGTGGG ATTGTGGAAG TCTTCAGTAT 
CCAGCATCCT GTAACTGTGG CTTTGTTGCC AAAGAAGGAA GTGATATAAT TGCATATGAC 
ATGTGCAGCG GTCAGCTACA TGAGTCACAG CCACACTTAT CCATAATAAG TAGAGACACA 
ACAGGAAGCA ATGTCAGAAT CACTGAATCC TACCTAGGAA GAAAAGTAAC AGTTTTGTTT 
TCTTCTGGAG CTTTTATTCG TGCTGATGTG AGTGAATGGG GAATGAGCAT AACACTTAGG 
GCTCCCAGTT CAGATTACAG AAATACAATG GGGCTCTGTG GCACCTTTGA TGGAAATGCA 
GAGAATGACT ATCACACTGC CAATGGGGTG GAAATACCAA ACAATTCTGA TGCTCTTTTT 
TCTTTTATAA AGGAATGGAG AATTCCACCA GGAGGTAGCT TGTTTGACAA CACACCTGCT 
TCATTACCAT CTTCTAGGAA AACGGACTTC TGTGACTGCA CATTTGAAGA TGCTGGATTA 
GATCCATCAG TACATAAACT AAAGGCAATT TCTAAATCGG AACTTCCCCT GTCTTGTAAA 
GACAGTGAAA ATGGTAGATA TCTTTCTTTG ATACCAGGAC TGGAAGTCAC TGCTGAGTAT 
CTCAGTCCTG CTGATCTTGT CAAAGGCTTA AACAAACGTT CATCTGTACA TGAAATGGAT 
TCCTCTACAC TTCTGCGGAA GGGGGATAAC CAAACCAAAC TTCACAAATC ATCATCAGTA 
AACAGACATG TCTCTGAAAT CTCAGAAATA AGTTTTGGAA TAGAAAAAGA AGGAACATCA 
GACATTAAAA GAATTTCTGA CAGTTACAGT AATCTTCACA AAAGTCAATC TGTTACAAGT 
AGAAGGAAGC GCCAAAGTTA TTATGAATAT CATCCAGTAT TTCTATTCCA AAGTCTAAGC 
CAAAGGGATT TAGAGGGGTA TAGGTACTTT TTCCCAGAGG ATCACACCAC TGGCGAAGAC 
AAGAAGTTTC TTCCTTCTTG GCCCACACCT TCAGGCCTCA CTGAGTCTAG CGCTCTGAGA 
CTGTGCCAGC AGGCAATAGC CAATTCCAGT ATAGGAAGAG CTTGTGGTGG CCTTCTTGGC 
AGAAGAATGC AAGATGCAAT CAATATCTGT GTTAAAGATT TACTGTTGAA AGATGACCTC 
AGATGGGCAG AAGCTTCATT AGCTCTTTTA GAGAATGAAT GTGAGAAGAG AGTTTTAGAA 
GAAATAAACT ACAATCAACA GGAACTTCAA GGGTTTGTTG AGAACATGCT TATTGCATTG 
AAGTGTCCCA GTCTGTGCAG TGGTAATGGA GAATGTACAG AATGGGGTTG TGCATGCTTT 
CAAGGCTATG GTTCGTACGA CTGCAGTATA CTGTCTGACC AAGCTCCAGA AATTACAGAG 
CTGGAAAATG CTGGGCTATG TGATATTCGT CAGTATGACT GCACATCAGT GAGGGCTTTT 
GGACATGGCT TCAGGGAATC ATCGAATCTG AAGTGTGAAA TTATCAGATT GCAATATACT 
GATAGCCAGT GGATTCCTGG AGAACCTCTA ATTACGTCAG CTGCCTTCCA AAATGTCAGG 
ACTGTCGACT GCGAGTTACC AAATAATGGC CCACAACCTG ATGTCGTGGA TCTGGTTGAT 
GATAAACCCA TTGCTTGGTG GAAAATAAAG ATTTCTAATG ATGGATTAAC ATACAGCAAT 
GCTAAAACGA TGATAATATA TGATGGAGCC TGTCAGATAT GTGAACTACA GGAATCTGGA 
TTATGTGCAC TAAAGGAGAA AACATGCAAC ATAGATGGAC TCTGTTATGG TGAAGGTGAC 
ACGAATCCAA TCAGCCCTTG CTTACTCTGC AGACCTGACA CATCAAGGTT AACTTGGTCA 
GTTGTTGAAA ACAACCAGCC TCCAGTGCTT GAAACTTTTC AGGGTATGCT ACAGACTTTT 
TATGGAGAAG ATTTTGTATA TCAGTTTTTG GCTTCAGATC CAGAGGGCTC TGCTATTCTT 
TTCACATTGG AGTCTGGTCC TGAAGGTGCC AGTCTTTCCC CATCAGGTCT CCTTTTGTGG 
AAAGCTGTGT CAAAGAATCC CCGTAAATTA ACATTTTCTT TGACAGATGA CTGCAATGCG 
GAGACTAAGG TAGAAATAGA GGTCAGGGTG AATTCATGTG ATTGCCTGAA TAATGGCTCA 
TGTGTGACAA ACATTAATTT CCCACCTGGA AGTGGGAGAT ACCTGTGTGT GTGTGTGGCT 
GGATTTGAAG GTGATCTCTG TCAAGTGAAT ACTGATGACT GCAAACTCAA CCGGTGTGGT 
ACTGGCAAAT GTGTGGATGG AGTAAACAGC TATTACTGTG AATGCCCACC TGAACTTCAA 
GGTGAAAATT GTCAAGATGA CATAGATGAA TGTGCATCCA ACCCTTGCTT CCCTGGAGTA 
TTTTGCTTCA ATACTTTTGG TTCTTACTAT TGCGGTTCAT GCCCAAATAA TCTACATGGA 
GATGGGAAGT CATGCTACGA AACCTTTGAA GATATTTATG CTGAAAGAAA AGATTCCACA 
GAAGCTGGAG AGAATAAAGG TTTTGAACAG TCATCATTTG ACAACGTCTT GAGCTCTGTT 
CCCAGTTCTA CCGATGTCCC CCAAAGATTT ATTTCTATAG AAATGGACAA TACTAGCACT 
CTATCAGCTT CCAGACCAAA AACAGTCACC ACTGAGAAGT CAGATAGCAC TCAGAGAAGT 
GCTTCTGTAC AATCAGATGT TACTGAAAAC ATTGCTCACG CTCTTGGTAG CACCAGTGGA 
CACCTTACTG GCACAGAAGG GGCTTCTCCA GAAAACACAG TGATGACTGC TTCTTCAAGG 
AGCCATGCTG TATCAGCTGA GAAGAAAACA AGGGCAAAAG TTGAGGCCTA CAGTCACTGG 
GAGACTAACA GGAAGGCTTC TCTTAGCAGT TCAGCGAATA CAAGCAAAGG GCTTTCTTTG 
CCACACAATG TATTTAAAGG TAGAAATGCT CACAGAGTTC AGCACTTGTT AGCCAAGCAT 
AGAGTGAGTC CTTTACCTTT TGCGCTTGTT GAAGTGACTG TCCCACCAGA AATGCTTCCT 
GAAGATTTAG ATGAAATAGT GCTTCCTAAA GCAGTAACCT GTGCTGACCT ACCCTGTTTT 
CCTGGAGTAA CTTGTGAGCC AAGTCAGGAT GACAGAGTCA AGTGTGGTCG TTGTCCTTAT 
GGTTACTATG GGGATGGCTT CAATTGTAGA GCAATATGTA GACAAACATG TGGCAAAAAT 
ATGGAGTGTG TGGCACCCAA TATTTGCAAG TGCAAGCCTG GTTATGCTGG TTATAACTGT 
CAGACAGCTC TATGCAGACC TGATTGCAAA AACCATGGAA AATGCATTAA GCCCAATGTC 
TGTGAATGTC TACCAGGTTA CAGTGGCTCC ACTTGTGAAG AAGCATACTG TAAACCATCC 
TGTAAAAATG GAGGCACGTG CTTGGCCAGA AATCTCTGTA CCTGCCCCTA TGGTTTTGTA 
GGACCAAGAT GTGATACAAT GATTTGCAAC AGACACTGTG AAAATGGCGG TGAATGTCTC 
ACTCCAGACA TCTGCCAGTG TAAATCTGGA TGGTATGGAC CAACGTGCAG TACAGCGATG 
TGTGACCCTG TATGTCTCAA TGGTGGTTCC TGTACTAAGC CAGGTGTTTG CCTCTGCCCA 
CATGGATTTT ATGGAGCCCG GTGTCAGAAT GCTGTCTGCA GGCCGCCTTG CAAGAATGGT 
GGCCACTGCA TGAGAAACAA CATTTGTACT TGTCCTGAAG GTTACACAGG CAGAAGATGT 
GAAAAAAGTG TCTGTGAGCC AACGTGCATG AATGGAGGAA GGTGCGTAGG GCCCAATGTT 
TGCTCTTGTC CTTCAGGGTG GAGTGGAAAA AAATGTAACA CACCAGTCTG TCTTCAGGAA 
TGTAAGAACG GTGGAGAGTG CATTGGACCA AGTACGTGTC ATTGTCCTCC ACAGTGGGAA 
GGAATCCAGT GTCAAACACC TGTGTGCAAT CAGAAGTGCC TGTTTGGAGG CAAGTGCGTG 
TCACCTAATG TGTGTTCCTG CCGTCCTGGT TATACTGGAG TCCTCTGTGG GAAGAAAGTT 
CAGGTACAAA GACGTCGTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025003133.1
CDS	365..5848
Translation	MAGGARLWLCAAVLGCLAAAKPRRRVLECHPGGHQILQSPYRSVDFDSSHLQQSAIQDLICDHSLAPGWYRFMIFDKPAEMPTKCVEMNHCGTQAPVWLSLKESESMPRPGEIKQLTACATWKFFFSTYKDCCLFQIPVSVRNCGDFFVYLLQPTQGCMGYCAEGKVAPSTSPSVSPPLPAIPEVAAELIKGSIYLRCTFGIPFANSSVGFIVTWSRLSPEGIKEELKHETTVHTFSLLELDGINVRLGERVYCSSSAFFMEKPSIQSSAVESKEFLAGIKIHPEAFDISEDGKEYRLSIESRIPIPCPEFNQVESDCRISLRLNTVDEGKEQLGLNLALSSCHVDLLQKPCRNGICSQAIIYFTAVTDFMQDGDRITRIAVEPISSENFLWNGYVPESTKITVKDLPTAYCYSFTDPHIITFDGRLYDNFKTGTFVLYKSTARDFEVHVRQWDCGSLQYPASCNCGFVAKEGSDIIAYDMCSGQLHESQPHLSIISRDTTGSNVRITESYLGRKVTVLFSSGAFIRADVSEWGMSITLRAPSSDYRNTMGLCGTFDGNAENDYHTANGVEIPNNSDALFSFIKEWRIPPGGSLFDNTPASLPSSRKTDFCDCTFEDAGLDPSVHKLKAISKSELPLSCKDSENGRYLSLIPGLEVTAEYLSPADLVKGLNKRSSVHEMDSSTLLRKGDNQTKLHKSSSVNRHVSEISEISFGIEKEGTSDIKRISDSYSNLHKSQSVTSRRKRQSYYEYHPVFLFQSLSQRDLEGYRYFFPEDHTTGEDKKFLPSWPTPSGLTESSALRLCQQAIANSSIGRACGGLLGRRMQDAINICVKDLLLKDDLRWAEASLALLENECEKRVLEEINYNQQELQGFVENMLIALKCPSLCSGNGECTEWGCACFQGYGSYDCSILSDQAPEITELENAGLCDIRQYDCTSVRAFGHGFRESSNLKCEIIRLQYTDSQWIPGEPLITSAAFQNVRTVDCELPNNGPQPDVVDLVDDKPIAWWKIKISNDGLTYSNAKTMIIYDGACQICELQESGLCALKEKTCNIDGLCYGEGDTNPISPCLLCRPDTSRLTWSVVENNQPPVLETFQGMLQTFYGEDFVYQFLASDPEGSAILFTLESGPEGASLSPSGLLLWKAVSKNPRKLTFSLTDDCNAETKVEIEVRVNSCDCLNNGSCVTNINFPPGSGRYLCVCVAGFEGDLCQVNTDDCKLNRCGTGKCVDGVNSYYCECPPELQGENCQDDIDECASNPCFPGVFCFNTFGSYYCGSCPNNLHGDGKSCYETFEDIYAERKDSTEAGENKGFEQSSFDNVLSSVPSSTDVPQRFISIEMDNTSTLSASRPKTVTTEKSDSTQRSASVQSDVTENIAHALGSTSGHLTGTEGASPENTVMTASSRSHAVSAEKKTRAKVEAYSHWETNRKASLSSSANTSKGLSLPHNVFKGRNAHRVQHLLAKHRVSPLPFALVEVTVPPEMLPEDLDEIVLPKAVTCADLPCFPGVTCEPSQDDRVKCGRCPYGYYGDGFNCRAICRQTCGKNMECVAPNICKCKPGYAGYNCQTALCRPDCKNHGKCIKPNVCECLPGYSGSTCEEAYCKPSCKNGGTCLARNLCTCPYGFVGPRCDTMICNRHCENGGECLTPDICQCKSGWYGPTCSTAMCDPVCLNGGSCTKPGVCLCPHGFYGARCQNAVCRPPCKNGGHCMRNNICTCPEGYTGRRCEKSVCEPTCMNGGRCVGPNVCSCPSGWSGKKCNTPVCLQECKNGGECIGPSTCHCPPQWEGIQCQTPVCNQKCLFGGKCVSPNVCSCRPGYTGVLCGKKVQVQRRRG

Target ORF information:

RefSeq Version	XM_025147365.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus von Willebrand factor D and EGF domains (VWDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147365.1

ORF Insert Sequence:

ATGGCGGGGG GCGCCCGCCT GTGGCTGTGC GCGGCCGTGC TCGGCTGCCT GGCGGCGGCG 
AAGCCCCGCC GGAGAGTTCT TGAATGCCAT CCTGGTGGAC ATCAGATCCT GCAGAGCCCT 
TACCGAAGTG TTGATTTTGA TTCATCCCAC CTCCAGCAGT CAGCTATTCA GGATTTGATA 
TGTGACCATT CCCTAGCACC AGGATGGTAT CGCTTTATGA TTTTTGATAA GCCTGCTGAG 
ATGCCAACCA AGTGTGTGGA GATGAATCAC TGTGGTACTC AAGCCCCTGT CTGGTTGTCT 
CTGAAAGAGT CAGAATCCAT GCCTCGTCCT GGTGAGATCA AGCAGCTCAC AGCCTGTGCA 
ACATGGAAGT TTTTCTTCAG CACTTACAAA GATTGCTGCC TTTTTCAAAT CCCTGTCAGT 
GTGAGGAACT GTGGAGATTT CTTTGTTTAC TTACTACAAC CCACACAAGG ATGCATGGGT 
TACTGTGCAG AAGGTAAGGT GGCTCCTTCA ACATCTCCGT CTGTGTCACC ACCACTACCA 
GCCATTCCTG AGGTTGCAGC AGAGTTGATC AAAGGGAGCA TTTACCTAAG GTGTACCTTT 
GGCATTCCTT TTGCAAACAG CTCAGTTGGA TTCATTGTGA CTTGGTCGAG ACTTTCTCCT 
GAAGGCATCA AAGAAGAACT GAAACATGAG ACAACAGTTC ATACATTCTC ACTCTTAGAG 
TTAGATGGGA TAAATGTCAG ACTTGGAGAA AGAGTCTACT GTAGCAGTTC TGCTTTCTTC 
ATGGAGAAGC CAAGCATACA GAGCAGTGCA GTTGAGAGCA AGGAATTTCT TGCTGGCATT 
AAGATACATC CAGAAGCGTT TGATATATCA GAAGATGGCA AGGAATACAG ACTGTCAATA 
GAAAGCAGAA TCCCTATTCC TTGTCCTGAA TTTAACCAGG TTGAAAGTGA CTGCAGAATA 
TCATTAAGAT TGAATACTGT TGATGAAGGT AAAGAACAAT TAGGTTTAAA CTTGGCTCTT 
TCTTCTTGTC ATGTGGATCT TCTCCAGAAA CCCTGCAGAA ATGGAATCTG TAGTCAAGCT 
ATTATTTATT TCACTGCTGT GACAGACTTT ATGCAGGATG GAGACAGAAT TACCAGAATT 
GCAGTTGAAC CTATATCCAG TGAGAATTTC CTGTGGAATG GCTATGTTCC AGAAAGCACA 
AAGATTACAG TAAAGGATCT ACCAACTGCC TACTGCTACT CTTTTACTGA CCCTCATATA 
ATTACGTTTG ATGGAAGACT CTATGACAAT TTTAAAACAG GAACATTTGT TCTCTACAAG 
AGTACAGCTC GAGATTTTGA AGTTCATGTT CGCCAGTGGG ATTGTGGAAG TCTTCAGTAT 
CCAGCATCCT GTAACTGTGG CTTTGTTGCC AAAGAAGGAA GTGATATAAT TGCATATGAC 
ATGTGCAGCG GTCAGCTACA TGAGTCACAG CCACACTTAT CCATAATAAG TAGAGACACA 
ACAGGAAGCA ATGTCAGAAT CACTGAATCC TACCTAGGAA GAAAAGTAAC AGTTTTGTTT 
TCTTCTGGAG CTTTTATTCG TGCTGATGTG AGTGAATGGG GAATGAGCAT AACACTTAGG 
GCTCCCAGTT CAGATTACAG AAATACAATG GGGCTCTGTG GCACCTTTGA TGGAAATGCA 
GAGAATGACT ATCACACTGC CAATGGGGTG GAAATACCAA ACAATTCTGA TGCTCTTTTT 
TCTTTTATAA AGGAATGGAG AATTCCACCA GGAGGTAGCT TGTTTGACAA CACACCTGCT 
TCATTACCAT CTTCTAGGAA AACGGACTTC TGTGACTGCA CATTTGAAGA TGCTGGATTA 
GATCCATCAG TACATAAACT AAAGGCAATT TCTAAATCGG AACTTCCCCT GTCTTGTAAA 
GACAGTGAAA ATGGTAGATA TCTTTCTTTG ATACCAGGAC TGGAAGTCAC TGCTGAGTAT 
CTCAGTCCTG CTGATCTTGT CAAAGGCTTA AACAAACGTT CATCTGTACA TGAAATGGAT 
TCCTCTACAC TTCTGCGGAA GGGGGATAAC CAAACCAAAC TTCACAAATC ATCATCAGTA 
AACAGACATG TCTCTGAAAT CTCAGAAATA AGTTTTGGAA TAGAAAAAGA AGGAACATCA 
GACATTAAAA GAATTTCTGA CAGTTACAGT AATCTTCACA AAAGTCAATC TGTTACAAGT 
AGAAGGAAGC GCCAAAGTTA TTATGAATAT CATCCAGTAT TTCTATTCCA AAGTCTAAGC 
CAAAGGGATT TAGAGGGGTA TAGGTACTTT TTCCCAGAGG ATCACACCAC TGGCGAAGAC 
AAGAAGTTTC TTCCTTCTTG GCCCACACCT TCAGGCCTCA CTGAGTCTAG CGCTCTGAGA 
CTGTGCCAGC AGGCAATAGC CAATTCCAGT ATAGGAAGAG CTTGTGGTGG CCTTCTTGGC 
AGAAGAATGC AAGATGCAAT CAATATCTGT GTTAAAGATT TACTGTTGAA AGATGACCTC 
AGATGGGCAG AAGCTTCATT AGCTCTTTTA GAGAATGAAT GTGAGAAGAG AGTTTTAGAA 
GAAATAAACT ACAATCAACA GGAACTTCAA GGGTTTGTTG AGAACATGCT TATTGCATTG 
AAGTGTCCCA GTCTGTGCAG TGGTAATGGA GAATGTACAG AATGGGGTTG TGCATGCTTT 
CAAGGCTATG GTTCGTACGA CTGCAGTATA CTGTCTGACC AAGCTCCAGA AATTACAGAG 
CTGGAAAATG CTGGGCTATG TGATATTCGT CAGTATGACT GCACATCAGT GAGGGCTTTT 
GGACATGGCT TCAGGGAATC ATCGAATCTG AAGTGTGAAA TTATCAGATT GCAATATACT 
GATAGCCAGT GGATTCCTGG AGAACCTCTA ATTACGTCAG CTGCCTTCCA AAATGTCAGG 
ACTGTCGACT GCGAGTTACC AAATAATGGC CCACAACCTG ATGTCGTGGA TCTGGTTGAT 
GATAAACCCA TTGCTTGGTG GAAAATAAAG ATTTCTAATG ATGGATTAAC ATACAGCAAT 
GCTAAAACGA TGATAATATA TGATGGAGCC TGTCAGATAT GTGAACTACA GGAATCTGGA 
TTATGTGCAC TAAAGGAGAA AACATGCAAC ATAGATGGAC TCTGTTATGG TGAAGGTGAC 
ACGAATCCAA TCAGCCCTTG CTTACTCTGC AGACCTGACA CATCAAGGTT AACTTGGTCA 
GTTGTTGAAA ACAACCAGCC TCCAGTGCTT GAAACTTTTC AGGGTATGCT ACAGACTTTT 
TATGGAGAAG ATTTTGTATA TCAGTTTTTG GCTTCAGATC CAGAGGGCTC TGCTATTCTT 
TTCACATTGG AGTCTGGTCC TGAAGGTGCC AGTCTTTCCC CATCAGGTCT CCTTTTGTGG 
AAAGCTGTGT CAAAGAATCC CCGTAAATTA ACATTTTCTT TGACAGATGA CTGCAATGCG 
GAGACTAAGG TAGAAATAGA GGTCAGGGTG AATTCATGTG ATTGCCTGAA TAATGGCTCA 
TGTGTGACAA ACATTAATTT CCCACCTGGA AGTGGGAGAT ACCTGTGTGT GTGTGTGGCT 
GGATTTGAAG GTGATCTCTG TCAAGTGAAT ACTGATGACT GCAAACTCAA CCGGTGTGGT 
ACTGGCAAAT GTGTGGATGG AGTAAACAGC TATTACTGTG AATGCCCACC TGAACTTCAA 
GGTGAAAATT GTCAAGATGA CATAGATGAA TGTGCATCCA ACCCTTGCTT CCCTGGAGTA 
TTTTGCTTCA ATACTTTTGG TTCTTACTAT TGCGGTTCAT GCCCAAATAA TCTACATGGA 
GATGGGAAGT CATGCTACGA AACCTTTGAA GATATTTATG CTGAAAGAAA AGATTCCACA 
GAAGCTGGAG AGAATAAAGG TTTTGAACAG TCATCATTTG ACAACGTCTT GAGCTCTGTT 
CCCAGTTCTA CCGATGTCCC CCAAAGATTT ATTTCTATAG AAATGGACAA TACTAGCACT 
CTATCAGCTT CCAGACCAAA AACAGTCACC ACTGAGAAGT CAGATAGCAC TCAGAGAAGT 
GCTTCTGTAC AATCAGATGT TACTGAAAAC ATTGCTCACG CTCTTGGTAG CACCAGTGGA 
CACCTTACTG GCACAGAAGG GGCTTCTCCA GAAAACACAG TGATGACTGC TTCTTCAAGG 
AGCCATGCTG TATCAGCTGA GAAGAAAACA AGGGCAAAAG TTGAGGCCTA CAGTCACTGG 
GAGACTAACA GGAAGGCTTC TCTTAGCAGT TCAGCGAATA CAAGCAAAGG GCTTTCTTTG 
CCACACAATG TATTTAAAGG TAGAAATGCT CACAGAGTTC AGCACTTGTT AGCCAAGCAT 
AGAGTGAGTC CTTTACCTTT TGCGCTTGTT GAAGTGACTG TCCCACCAGA AATGCTTCCT 
GAAGATTTAG ATGAAATAGT GCTTCCTAAA GCAGTAACCT GTGCTGACCT ACCCTGTTTT 
CCTGGAGTAA CTTGTGAGCC AAGTCAGGAT GACAGAGTCA AGTGTGGTCG TTGTCCTTAT 
GGTTACTATG GGGATGGCTT CAATTGTAGA GCAATATGTA GACAAACATG TGGCAAAAAT 
ATGGAGTGTG TGGCACCCAA TATTTGCAAG TGCAAGCCTG GTTATGCTGG TTATAACTGT 
CAGACAGCTC TATGCAGACC TGATTGCAAA AACCATGGAA AATGCATTAA GCCCAATGTC 
TGTGAATGTC TACCAGGTTA CAGTGGCTCC ACTTGTGAAG AAGCATACTG TAAACCATCC 
TGTAAAAATG GAGGCACGTG CTTGGCCAGA AATCTCTGTA CCTGCCCCTA TGGTTTTGTA 
GGACCAAGAT GTGATACAAT GATTTGCAAC AGACACTGTG AAAATGGCGG TGAATGTCTC 
ACTCCAGACA TCTGCCAGTG TAAATCTGGA TGGTATGGAC CAACGTGCAG TACAGCGATG 
TGTGACCCTG TATGTCTCAA TGGTGGTTCC TGTACTAAGC CAGGTGTTTG CCTCTGCCCA 
CATGGATTTT ATGGAGCCCG GTGTCAGAAT GCTGTCTGCA GGCCGCCTTG CAAGAATGGT 
GGCCACTGCA TGAGAAACAA CATTTGTACT TGTCCTGAAG GTTACACAGG CAGAAGATGT 
GAAAAAAGTG TCTGTGAGCC AACGTGCATG AATGGAGGAA GGTGCGTAGG GCCCAATGTT 
TGCTCTTGTC CTTCAGGGTG GAGTGGAAAA AAATGTAACA CACCAGTCTG TCTTCAGGAA 
TGTAAGAACG GTGGAGAGTG CATTGGACCA AGTACGTGTC ATTGTCCTCC ACAGTGGGAA 
GGAATCCAGT GTCAAACACC TGTGTGCAAT CAGAAGTGCC TGTTTGGAGG CAAGTGCGTG 
TCACCTAATG TGTGTTCCTG CCGTCCTGGT TATACTGGAG TCCTCTGTGG GAAGAAAGTT 
CAGGTACAAA GACGTCGTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VWDE ( XM_015281603.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137089.1 Gallus gallus von Willebrand factor D and EGF domains (VWDE), mRNA.

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CloneID	OGa31683
Clone ID Related Accession (Same CDS sequence)	XM_015281603.1
Accession Version	XM_015281603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	von Willebrand factor D and EGF domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGGGG GCGCCCGCCT GTGGCTGTGC GCGGCCGTGC TCGGCTGCCT GGCGGCGGCG 
AAGCCCCGCC GGAGAGTTCT TGAATGCCAT CCTGGTGGAC ATCAGATCCT GCAGAGCCCT 
TACCGAAGTG TTGATTTTGA TTCATCCCAC CTCCAGCAGT CAGCTATTCA GGATTTGATA 
TGTGACCATT CCCTAGCACC AGGATGGTAT CGCTTTATGA TTTTTGATAA GCCTGCTGAG 
ATGCCAACCA AGTGTGTGGA GATGAATCAC TGTGGTACTC AAGCCCCTGT CTGGTTGTCT 
CTGAAAGAGT CAGAATCCAT GCCTCGTCCT GGTGAGATCA AGCAGCTCAC AGCCTGTGCA 
ACATGGAAGT TTTTCTTCAG CACTTACAAA GATTGCTGCC TTTTTCAAAT CCCTGTCAGT 
GTGAGGAACT GTGGAGATTT CTTTGTTTAC TTACTACAAC CCACACAAGG ATGCATGGGT 
TACTGTGCAG AAGGTAAGGT GGCTCCTTCA ACATCTCCGT CTGTGTCACC ACCACTACCA 
GCCATTCCTG AGGTTGCAGC AGAGTTGATC AAAGGGAGCA TTTACCTAAG GTGTACCTTT 
GGCATTCCTT TTGCAAACAG CTCAGTTGGA TTCATTGTGA CTTGGTCGAG ACTTTCTCCT 
GAAGGCATCA AAGAAGAACT GAAACATGAG ACAACAGTTC ATACATTCTC ACTCTTAGAG 
TTAGATGGGA TAAATGTCAG ACTTGGAGAA AGAGTCTACT GTAGCAGTTC TGCTTTCTTC 
ATGGAGAAGC CAAGCATACA GAGCAGTGCA GTTGAGAGCA AGGAATTTCT TGCTGGCATT 
AAGATACATC CAGAAGCGTT TGATATATCA GAAGATGGCA AGGAATACAG ACTGTCAATA 
GAAAGCAGAA TCCCTATTCC TTGTCCTGAA TTTAACCAGG TTGAAAGTGA CTGCAGAATA 
TCATTAAGAT TGAATACTGT TGATGAAGGT AAAGAACAAT TAGGTTTAAA CTTGGCTCTT 
TCTTCTTGTC ATGTGGATCT TCTCCAGAAA CCCTGCAGAA ATGGAATCTG TAGTCAAGCT 
ATTATTTATT TCACTGCTGT GACAGACTTT ATGCAGGATG GAGACAGAAT TACCAGAATT 
GCAGTTGAAC CTATATCCAG TGAGAATTTC CTGTGGAATG GCTATGTTCC AGAAAGCACA 
AAGATTACAG TAAAGGATCT ACCAACTGCC TACTGCTACT CTTTTACTGA CCCTCATATA 
ATTACGTTTG ATGGAAGACT CTATGACAAT TTTAAAACAG GAACATTTGT TCTCTACAAG 
AGTACAGCTC GAGATTTTGA AGTTCATGTT CGCCAGTGGG ATTGTGGAAG TCTTCAGTAT 
CCAGCATCCT GTAACTGTGG CTTTGTTGCC AAAGAAGGAA GTGATATAAT TGCATATGAC 
ATGTGCAGCG GTCAGCTACA TGAGTCACAG CCACACTTAT CCATAATAAG TAGAGACACA 
ACAGGAAGCA ATGTCAGAAT CACTGAATCC TACCTAGGAA GAAAAGTAAC AGTTTTGTTT 
TCTTCTGGAG CTTTTATTCG TGCTGATGTG AGTGAATGGG GAATGAGCAT AACACTTAGG 
GCTCCCAGTT CAGATTACAG AAATACAATG GGGCTCTGTG GCACCTTTGA TGGAAATGCA 
GAGAATGACT ATCACACTGC CAATGGGGTG GAAATACCAA ACAATTCTGA TGCTCTTTTT 
TCTTTTATAA AGGAATGGAG AATTCCACCA GGAGGTAGCT TGTTTGACAA CACACCTGCT 
TCATTACCAT CTTCTAGGAA AACGGACTTC TGTGACTGCA CATTTGAAGA TGCTGGATTA 
GATCCATCAG TACATAAACT AAAGGCAATT TCTAAATCGG AACTTCCCCT GTCTTGTAAA 
GACAGTGAAA ATGGTAGATA TCTTTCTTTG ATACCAGGAC TGGAAGTCAC TGCTGAGTAT 
CTCAGTCCTG CTGATCTTGT CAAAGGCTTA AACAAACGTT CATCTGTACA TGAAATGGAT 
TCCTCTACAC TTCTGCGGAA GGGGGATAAC CAAACCAAAC TTCACAAATC ATCATCAGTA 
AACAGACATG TCTCTGAAAT CTCAGAAATA AGTTTTGGAA TAGAAAAAGA AGGAACATCA 
GACATTAAAA GAATTTCTGA CAGTTACAGT AATCTTCACA AAAGTCAATC TGTTACAAGT 
AGAAGGAAGC GCCAAAGTTA TTATGAATAT CATCCAGTAT TTCTATTCCA AAGTCTAAGC 
CAAAGGGATT TAGAGGGGTA TAGGTACTTT TTCCCAGAGG ATCACACCAC TGGCGAAGAC 
AAGAAGTTTC TTCCTTCTTG GCCCACACCT TCAGGCCTCA CTGAGTCTAG CGCTCTGAGA 
CTGTGCCAGC AGGCAATAGC CAATTCCAGT ATAGGAAGAG CTTGTGGTGG CCTTCTTGGC 
AGAAGAATGC AAGATGCAAT CAATATCTGT GTTAAAGATT TACTGTTGAA AGATGACCTC 
AGATGGGCAG AAGCTTCATT AGCTCTTTTA GAGAATGAAT GTGAGAAGAG AGTTTTAGAA 
GAAATAAACT ACAATCAACA GGAACTTCAA GGGTTTGTTG AGAACATGCT TATTGCATTG 
AAGTGTCCCA GTCTGTGCAG TGGTAATGGA GAATGTACAG AATGGGGTTG TGCATGCTTT 
CAAGGCTATG GTTCGTACGA CTGCAGTATA CTGTCTGACC AAGCTCCAGA AATTACAGAG 
CTGGAAAATG CTGGGCTATG TGATATTCGT CAGTATGACT GCACATCAGT GAGGGCTTTT 
GGACATGGCT TCAGGGAATC ATCGAATCTG AAGTGTGAAA TTATCAGATT GCAATATACT 
GATAGCCAGT GGATTCCTGG AGAACCTCTA ATTACGTCAG CTGCCTTCCA AAATGTCAGG 
ACTGTCGACT GCGAGTTACC AAATAATGGC CCACAACCTG ATGTCGTGGA TCTGGTTGAT 
GATAAACCCA TTGCTTGGTG GAAAATAAAG ATTTCTAATG ATGGATTAAC ATACAGCAAT 
GCTAAAACGA TGATAATATA TGATGGAGCC TGTCAGATAT GTGAACTACA GGAATCTGGA 
TTATGTGCAC TAAAGGAGAA AACATGCAAC ATAGATGGAC TCTGTTATGG TGAAGGTGAC 
ACGAATCCAA TCAGCCCTTG CTTACTCTGC AGACCTGACA CATCAAGGTT AACTTGGTCA 
GTTGTTGAAA ACAACCAGCC TCCAGTGCTT GAAACTTTTC AGGGTATGCT ACAGACTTTT 
TATGGAGAAG ATTTTGTATA TCAGTTTTTG GCTTCAGATC CAGAGGGCTC TGCTATTCTT 
TTCACATTGG AGTCTGGTCC TGAAGGTGCC AGTCTTTCCC CATCAGGTCT CCTTTTGTGG 
AAAGCTGTGT CAAAGAATCC CCGTAAATTA ACATTTTCTT TGACAGATGA CTGCAATGCG 
GAGACTAAGG TAGAAATAGA GGTCAGGGTG AATTCATGTG ATTGCCTGAA TAATGGCTCA 
TGTGTGACAA ACATTAATTT CCCACCTGGA AGTGGGAGAT ACCTGTGTGT GTGTGTGGCT 
GGATTTGAAG GTGATCTCTG TCAAGTGAAT ACTGATGACT GCAAACTCAA CCGGTGTGGT 
ACTGGCAAAT GTGTGGATGG AGTAAACAGC TATTACTGTG AATGCCCACC TGAACTTCAA 
GGTGAAAATT GTCAAGATGA CATAGATGAA TGTGCATCCA ACCCTTGCTT CCCTGGAGTA 
TTTTGCTTCA ATACTTTTGG TTCTTACTAT TGCGGTTCAT GCCCAAATAA TCTACATGGA 
GATGGGAAGT CATGCTACGA AACCTTTGAA GATATTTATG CTGAAAGAAA AGATTCCACA 
GAAGCTGGAG AGAATAAAGG TGATTTTATT TCATCAAGAT ATTCTACCGA TGTCCCCCAA 
AGATTTATTT CTATAGAAAT GGACAATACT AGCACTCTAT CAGCTTCCAG ACCAAAAACA 
GTCACCACTG AGAAGTCAGA TAGCACTCAG AGAAGTGCTT CTGTACAATC AGATGTTACT 
GAAAACATTG CTCACGCTCT TGGTAGCACC AGTGGACACC TTACTGGCAC AGAAGGGGCT 
TCTCCAGAAA ACACAGTGAT GACTGCTTCT TCAAGGAGCC ATGCTGTATC AGCTGAGAAG 
AAAACAAGGG CAAAAGTTGA GGCCTACAGT CACTGGGAGA CTAACAGGAA GGCTTCTCTT 
AGCAGTTCAG CGAATACAAG CAAAGGGCTT TCTTTGCCAC ACAATGTATT TAAAGGTAGA 
AATGCTCACA GAGTTCAGCA CTTGTTAGCC AAGCATAGAG TGAGTCCTTT ACCTTTTGCG 
CTTGTTGAAG TGACTGTCCC ACCAGAAATG CTTCCTGAAG ATTTAGATGA AATAGTGCTT 
CCTAAAGCAG TAACCTGTGC TGACCTACCC TGTTTTCCTG GAGTAACTTG TGAGCCAAGT 
CAGGATGACA GAGTCAAGTG TGGTCGTTGT CCTTATGGTT ACTATGGGGA TGGCTTCAAT 
TGTAGAGGAA ACTGTCATCT TCAGGCTTGT ATTGGTTCAG ACATAGTTAG GCTGAAATGC 
CACTGTTTGT TTCTTTATTC CTTTCACTGT TTTCTTAATT GCCTTTCAGC AATATGTAGA 
CAAACATGTG GCAAAAATAT GGAGTGTGTG GCACCCAATA TTTGCAAGTG CAAGCCTGGT 
TATGCTGGTT ATAACTGTCA GACAGCTCTA TGCAGACCTG ATTGCAAAAA CCATGGAAAA 
TGCATTAAGC CCAATGTCTG TGAATGTCTA CCAGGTTACA GTGGCTCCAC TTGTGAAGAA 
GCATACTGTA AACCATCCTG TAAAAATGGA GGCACGTGCT TGGCCAGAAA TCTCTGTACC 
TGCCCCTATG GTTTTGTAGG ACCAAGATGT GATACAATGA TTTGCAACAG ACACTGTGAA 
AATGGCGGTG AATGTCTCAC TCCAGACATC TGCCAGTGTA AATCTGGATG GTATGGACCA 
ACGTGCAGTA CAGCGATGTG TGACCCTGTA TGTCTCAATG GTGGTTCCTG TACTAAGCCA 
GGTGTTTGCC TCTGCCCACA TGGATTTTAT GGAGCCCGGT GTCAGAATGC TGTCTGCAGG 
CCGCCTTGCA AGAATGGTGG CCACTGCATG AGAAACAACA TTTGTACTTG TCCTGAAGGT 
TACACAGGCA GAAGATGTGA AAAAAGTGTC TGTGAGCCAA CGTGCATGAA TGGAGGAAGG 
TGCGTAGGGC CCAATGTTTG CTCTTGTCCT TCAGGGTGGA GTGGAAAAAA ATGTAACACA 
CCAGTCTGTC TTCAGGAATG TAAGAACGGT GGAGAGTGCA TTGGACCAAG TACGTGTCAT 
TGTCCTCCAC AGTGGGAAGG AATCCAGTGT CAAACACCTG TGTGCAATCA GAAGTGCCTG 
TTTGGAGGCA AGTGCGTGTC ACCTAATGTG TGTTCCTGCC GTCCTGGTTA TACTGGAGTC 
CTCTGTGGGA AGAAAGTTCA GGTACAAAGA CGTCGTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137089.1
CDS	1..5562
Translation	MAGGARLWLCAAVLGCLAAAKPRRRVLECHPGGHQILQSPYRSVDFDSSHLQQSAIQDLICDHSLAPGWYRFMIFDKPAEMPTKCVEMNHCGTQAPVWLSLKESESMPRPGEIKQLTACATWKFFFSTYKDCCLFQIPVSVRNCGDFFVYLLQPTQGCMGYCAEGKVAPSTSPSVSPPLPAIPEVAAELIKGSIYLRCTFGIPFANSSVGFIVTWSRLSPEGIKEELKHETTVHTFSLLELDGINVRLGERVYCSSSAFFMEKPSIQSSAVESKEFLAGIKIHPEAFDISEDGKEYRLSIESRIPIPCPEFNQVESDCRISLRLNTVDEGKEQLGLNLALSSCHVDLLQKPCRNGICSQAIIYFTAVTDFMQDGDRITRIAVEPISSENFLWNGYVPESTKITVKDLPTAYCYSFTDPHIITFDGRLYDNFKTGTFVLYKSTARDFEVHVRQWDCGSLQYPASCNCGFVAKEGSDIIAYDMCSGQLHESQPHLSIISRDTTGSNVRITESYLGRKVTVLFSSGAFIRADVSEWGMSITLRAPSSDYRNTMGLCGTFDGNAENDYHTANGVEIPNNSDALFSFIKEWRIPPGGSLFDNTPASLPSSRKTDFCDCTFEDAGLDPSVHKLKAISKSELPLSCKDSENGRYLSLIPGLEVTAEYLSPADLVKGLNKRSSVHEMDSSTLLRKGDNQTKLHKSSSVNRHVSEISEISFGIEKEGTSDIKRISDSYSNLHKSQSVTSRRKRQSYYEYHPVFLFQSLSQRDLEGYRYFFPEDHTTGEDKKFLPSWPTPSGLTESSALRLCQQAIANSSIGRACGGLLGRRMQDAINICVKDLLLKDDLRWAEASLALLENECEKRVLEEINYNQQELQGFVENMLIALKCPSLCSGNGECTEWGCACFQGYGSYDCSILSDQAPEITELENAGLCDIRQYDCTSVRAFGHGFRESSNLKCEIIRLQYTDSQWIPGEPLITSAAFQNVRTVDCELPNNGPQPDVVDLVDDKPIAWWKIKISNDGLTYSNAKTMIIYDGACQICELQESGLCALKEKTCNIDGLCYGEGDTNPISPCLLCRPDTSRLTWSVVENNQPPVLETFQGMLQTFYGEDFVYQFLASDPEGSAILFTLESGPEGASLSPSGLLLWKAVSKNPRKLTFSLTDDCNAETKVEIEVRVNSCDCLNNGSCVTNINFPPGSGRYLCVCVAGFEGDLCQVNTDDCKLNRCGTGKCVDGVNSYYCECPPELQGENCQDDIDECASNPCFPGVFCFNTFGSYYCGSCPNNLHGDGKSCYETFEDIYAERKDSTEAGENKGDFISSRYSTDVPQRFISIEMDNTSTLSASRPKTVTTEKSDSTQRSASVQSDVTENIAHALGSTSGHLTGTEGASPENTVMTASSRSHAVSAEKKTRAKVEAYSHWETNRKASLSSSANTSKGLSLPHNVFKGRNAHRVQHLLAKHRVSPLPFALVEVTVPPEMLPEDLDEIVLPKAVTCADLPCFPGVTCEPSQDDRVKCGRCPYGYYGDGFNCRGNCHLQACIGSDIVRLKCHCLFLYSFHCFLNCLSAICRQTCGKNMECVAPNICKCKPGYAGYNCQTALCRPDCKNHGKCIKPNVCECLPGYSGSTCEEAYCKPSCKNGGTCLARNLCTCPYGFVGPRCDTMICNRHCENGGECLTPDICQCKSGWYGPTCSTAMCDPVCLNGGSCTKPGVCLCPHGFYGARCQNAVCRPPCKNGGHCMRNNICTCPEGYTGRRCEKSVCEPTCMNGGRCVGPNVCSCPSGWSGKKCNTPVCLQECKNGGECIGPSTCHCPPQWEGIQCQTPVCNQKCLFGGKCVSPNVCSCRPGYTGVLCGKKVQVQRRRG

Target ORF information:

RefSeq Version	XM_015281603.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus von Willebrand factor D and EGF domains (VWDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281603.1

ORF Insert Sequence:

ATGGCGGGGG GCGCCCGCCT GTGGCTGTGC GCGGCCGTGC TCGGCTGCCT GGCGGCGGCG 
AAGCCCCGCC GGAGAGTTCT TGAATGCCAT CCTGGTGGAC ATCAGATCCT GCAGAGCCCT 
TACCGAAGTG TTGATTTTGA TTCATCCCAC CTCCAGCAGT CAGCTATTCA GGATTTGATA 
TGTGACCATT CCCTAGCACC AGGATGGTAT CGCTTTATGA TTTTTGATAA GCCTGCTGAG 
ATGCCAACCA AGTGTGTGGA GATGAATCAC TGTGGTACTC AAGCCCCTGT CTGGTTGTCT 
CTGAAAGAGT CAGAATCCAT GCCTCGTCCT GGTGAGATCA AGCAGCTCAC AGCCTGTGCA 
ACATGGAAGT TTTTCTTCAG CACTTACAAA GATTGCTGCC TTTTTCAAAT CCCTGTCAGT 
GTGAGGAACT GTGGAGATTT CTTTGTTTAC TTACTACAAC CCACACAAGG ATGCATGGGT 
TACTGTGCAG AAGGTAAGGT GGCTCCTTCA ACATCTCCGT CTGTGTCACC ACCACTACCA 
GCCATTCCTG AGGTTGCAGC AGAGTTGATC AAAGGGAGCA TTTACCTAAG GTGTACCTTT 
GGCATTCCTT TTGCAAACAG CTCAGTTGGA TTCATTGTGA CTTGGTCGAG ACTTTCTCCT 
GAAGGCATCA AAGAAGAACT GAAACATGAG ACAACAGTTC ATACATTCTC ACTCTTAGAG 
TTAGATGGGA TAAATGTCAG ACTTGGAGAA AGAGTCTACT GTAGCAGTTC TGCTTTCTTC 
ATGGAGAAGC CAAGCATACA GAGCAGTGCA GTTGAGAGCA AGGAATTTCT TGCTGGCATT 
AAGATACATC CAGAAGCGTT TGATATATCA GAAGATGGCA AGGAATACAG ACTGTCAATA 
GAAAGCAGAA TCCCTATTCC TTGTCCTGAA TTTAACCAGG TTGAAAGTGA CTGCAGAATA 
TCATTAAGAT TGAATACTGT TGATGAAGGT AAAGAACAAT TAGGTTTAAA CTTGGCTCTT 
TCTTCTTGTC ATGTGGATCT TCTCCAGAAA CCCTGCAGAA ATGGAATCTG TAGTCAAGCT 
ATTATTTATT TCACTGCTGT GACAGACTTT ATGCAGGATG GAGACAGAAT TACCAGAATT 
GCAGTTGAAC CTATATCCAG TGAGAATTTC CTGTGGAATG GCTATGTTCC AGAAAGCACA 
AAGATTACAG TAAAGGATCT ACCAACTGCC TACTGCTACT CTTTTACTGA CCCTCATATA 
ATTACGTTTG ATGGAAGACT CTATGACAAT TTTAAAACAG GAACATTTGT TCTCTACAAG 
AGTACAGCTC GAGATTTTGA AGTTCATGTT CGCCAGTGGG ATTGTGGAAG TCTTCAGTAT 
CCAGCATCCT GTAACTGTGG CTTTGTTGCC AAAGAAGGAA GTGATATAAT TGCATATGAC 
ATGTGCAGCG GTCAGCTACA TGAGTCACAG CCACACTTAT CCATAATAAG TAGAGACACA 
ACAGGAAGCA ATGTCAGAAT CACTGAATCC TACCTAGGAA GAAAAGTAAC AGTTTTGTTT 
TCTTCTGGAG CTTTTATTCG TGCTGATGTG AGTGAATGGG GAATGAGCAT AACACTTAGG 
GCTCCCAGTT CAGATTACAG AAATACAATG GGGCTCTGTG GCACCTTTGA TGGAAATGCA 
GAGAATGACT ATCACACTGC CAATGGGGTG GAAATACCAA ACAATTCTGA TGCTCTTTTT 
TCTTTTATAA AGGAATGGAG AATTCCACCA GGAGGTAGCT TGTTTGACAA CACACCTGCT 
TCATTACCAT CTTCTAGGAA AACGGACTTC TGTGACTGCA CATTTGAAGA TGCTGGATTA 
GATCCATCAG TACATAAACT AAAGGCAATT TCTAAATCGG AACTTCCCCT GTCTTGTAAA 
GACAGTGAAA ATGGTAGATA TCTTTCTTTG ATACCAGGAC TGGAAGTCAC TGCTGAGTAT 
CTCAGTCCTG CTGATCTTGT CAAAGGCTTA AACAAACGTT CATCTGTACA TGAAATGGAT 
TCCTCTACAC TTCTGCGGAA GGGGGATAAC CAAACCAAAC TTCACAAATC ATCATCAGTA 
AACAGACATG TCTCTGAAAT CTCAGAAATA AGTTTTGGAA TAGAAAAAGA AGGAACATCA 
GACATTAAAA GAATTTCTGA CAGTTACAGT AATCTTCACA AAAGTCAATC TGTTACAAGT 
AGAAGGAAGC GCCAAAGTTA TTATGAATAT CATCCAGTAT TTCTATTCCA AAGTCTAAGC 
CAAAGGGATT TAGAGGGGTA TAGGTACTTT TTCCCAGAGG ATCACACCAC TGGCGAAGAC 
AAGAAGTTTC TTCCTTCTTG GCCCACACCT TCAGGCCTCA CTGAGTCTAG CGCTCTGAGA 
CTGTGCCAGC AGGCAATAGC CAATTCCAGT ATAGGAAGAG CTTGTGGTGG CCTTCTTGGC 
AGAAGAATGC AAGATGCAAT CAATATCTGT GTTAAAGATT TACTGTTGAA AGATGACCTC 
AGATGGGCAG AAGCTTCATT AGCTCTTTTA GAGAATGAAT GTGAGAAGAG AGTTTTAGAA 
GAAATAAACT ACAATCAACA GGAACTTCAA GGGTTTGTTG AGAACATGCT TATTGCATTG 
AAGTGTCCCA GTCTGTGCAG TGGTAATGGA GAATGTACAG AATGGGGTTG TGCATGCTTT 
CAAGGCTATG GTTCGTACGA CTGCAGTATA CTGTCTGACC AAGCTCCAGA AATTACAGAG 
CTGGAAAATG CTGGGCTATG TGATATTCGT CAGTATGACT GCACATCAGT GAGGGCTTTT 
GGACATGGCT TCAGGGAATC ATCGAATCTG AAGTGTGAAA TTATCAGATT GCAATATACT 
GATAGCCAGT GGATTCCTGG AGAACCTCTA ATTACGTCAG CTGCCTTCCA AAATGTCAGG 
ACTGTCGACT GCGAGTTACC AAATAATGGC CCACAACCTG ATGTCGTGGA TCTGGTTGAT 
GATAAACCCA TTGCTTGGTG GAAAATAAAG ATTTCTAATG ATGGATTAAC ATACAGCAAT 
GCTAAAACGA TGATAATATA TGATGGAGCC TGTCAGATAT GTGAACTACA GGAATCTGGA 
TTATGTGCAC TAAAGGAGAA AACATGCAAC ATAGATGGAC TCTGTTATGG TGAAGGTGAC 
ACGAATCCAA TCAGCCCTTG CTTACTCTGC AGACCTGACA CATCAAGGTT AACTTGGTCA 
GTTGTTGAAA ACAACCAGCC TCCAGTGCTT GAAACTTTTC AGGGTATGCT ACAGACTTTT 
TATGGAGAAG ATTTTGTATA TCAGTTTTTG GCTTCAGATC CAGAGGGCTC TGCTATTCTT 
TTCACATTGG AGTCTGGTCC TGAAGGTGCC AGTCTTTCCC CATCAGGTCT CCTTTTGTGG 
AAAGCTGTGT CAAAGAATCC CCGTAAATTA ACATTTTCTT TGACAGATGA CTGCAATGCG 
GAGACTAAGG TAGAAATAGA GGTCAGGGTG AATTCATGTG ATTGCCTGAA TAATGGCTCA 
TGTGTGACAA ACATTAATTT CCCACCTGGA AGTGGGAGAT ACCTGTGTGT GTGTGTGGCT 
GGATTTGAAG GTGATCTCTG TCAAGTGAAT ACTGATGACT GCAAACTCAA CCGGTGTGGT 
ACTGGCAAAT GTGTGGATGG AGTAAACAGC TATTACTGTG AATGCCCACC TGAACTTCAA 
GGTGAAAATT GTCAAGATGA CATAGATGAA TGTGCATCCA ACCCTTGCTT CCCTGGAGTA 
TTTTGCTTCA ATACTTTTGG TTCTTACTAT TGCGGTTCAT GCCCAAATAA TCTACATGGA 
GATGGGAAGT CATGCTACGA AACCTTTGAA GATATTTATG CTGAAAGAAA AGATTCCACA 
GAAGCTGGAG AGAATAAAGG TGATTTTATT TCATCAAGAT ATTCTACCGA TGTCCCCCAA 
AGATTTATTT CTATAGAAAT GGACAATACT AGCACTCTAT CAGCTTCCAG ACCAAAAACA 
GTCACCACTG AGAAGTCAGA TAGCACTCAG AGAAGTGCTT CTGTACAATC AGATGTTACT 
GAAAACATTG CTCACGCTCT TGGTAGCACC AGTGGACACC TTACTGGCAC AGAAGGGGCT 
TCTCCAGAAA ACACAGTGAT GACTGCTTCT TCAAGGAGCC ATGCTGTATC AGCTGAGAAG 
AAAACAAGGG CAAAAGTTGA GGCCTACAGT CACTGGGAGA CTAACAGGAA GGCTTCTCTT 
AGCAGTTCAG CGAATACAAG CAAAGGGCTT TCTTTGCCAC ACAATGTATT TAAAGGTAGA 
AATGCTCACA GAGTTCAGCA CTTGTTAGCC AAGCATAGAG TGAGTCCTTT ACCTTTTGCG 
CTTGTTGAAG TGACTGTCCC ACCAGAAATG CTTCCTGAAG ATTTAGATGA AATAGTGCTT 
CCTAAAGCAG TAACCTGTGC TGACCTACCC TGTTTTCCTG GAGTAACTTG TGAGCCAAGT 
CAGGATGACA GAGTCAAGTG TGGTCGTTGT CCTTATGGTT ACTATGGGGA TGGCTTCAAT 
TGTAGAGGAA ACTGTCATCT TCAGGCTTGT ATTGGTTCAG ACATAGTTAG GCTGAAATGC 
CACTGTTTGT TTCTTTATTC CTTTCACTGT TTTCTTAATT GCCTTTCAGC AATATGTAGA 
CAAACATGTG GCAAAAATAT GGAGTGTGTG GCACCCAATA TTTGCAAGTG CAAGCCTGGT 
TATGCTGGTT ATAACTGTCA GACAGCTCTA TGCAGACCTG ATTGCAAAAA CCATGGAAAA 
TGCATTAAGC CCAATGTCTG TGAATGTCTA CCAGGTTACA GTGGCTCCAC TTGTGAAGAA 
GCATACTGTA AACCATCCTG TAAAAATGGA GGCACGTGCT TGGCCAGAAA TCTCTGTACC 
TGCCCCTATG GTTTTGTAGG ACCAAGATGT GATACAATGA TTTGCAACAG ACACTGTGAA 
AATGGCGGTG AATGTCTCAC TCCAGACATC TGCCAGTGTA AATCTGGATG GTATGGACCA 
ACGTGCAGTA CAGCGATGTG TGACCCTGTA TGTCTCAATG GTGGTTCCTG TACTAAGCCA 
GGTGTTTGCC TCTGCCCACA TGGATTTTAT GGAGCCCGGT GTCAGAATGC TGTCTGCAGG 
CCGCCTTGCA AGAATGGTGG CCACTGCATG AGAAACAACA TTTGTACTTG TCCTGAAGGT 
TACACAGGCA GAAGATGTGA AAAAAGTGTC TGTGAGCCAA CGTGCATGAA TGGAGGAAGG 
TGCGTAGGGC CCAATGTTTG CTCTTGTCCT TCAGGGTGGA GTGGAAAAAA ATGTAACACA 
CCAGTCTGTC TTCAGGAATG TAAGAACGGT GGAGAGTGCA TTGGACCAAG TACGTGTCAT 
TGTCCTCCAC AGTGGGAAGG AATCCAGTGT CAAACACCTG TGTGCAATCA GAAGTGCCTG 
TTTGGAGGCA AGTGCGTGTC ACCTAATGTG TGTTCCTGCC GTCCTGGTTA TACTGGAGTC 
CTCTGTGGGA AGAAAGTTCA GGTACAAAGA CGTCGTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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VWDE cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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