COL22A1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL22A1
Entrez Gene ID	420315
Full Name	collagen type XXII alpha 1 chain
General protein information	Preferred Names collagen type XXII alpha 1 chain Names collagen alpha-1(XXII) chain collagen, type XXII, alpha 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015283174.1	XP_015138660.1	collagen alpha-1(XXII) chain isoform X1
NM_001139439.1	NP_001132911.1	collagen alpha-1(XXII) chain precursor
XM_025147123.1	XP_025002891.1	collagen alpha-1(XXII) chain isoform X2
XM_025147124.1	XP_025002892.1	collagen alpha-1(XXII) chain isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL22A1	XP_528236.3
Canis lupus familiaris (dog)	COL22A1	XP_005628047.1
Gallus gallus (chicken)	COL22A1	NP_001132911.1
Macaca mulatta (Rhesus monkey)	LOC100425803	XP_002805544.1
Homo sapiens (human)	COL22A1	NP_690848.1
Mus musculus (house mouse)	Col22a1	NP_081450.1
Danio rerio (zebrafish)	prp	XP_002665520.3
Bos taurus (cattle)	COL22A1	XP_002692646.2
Rattus norvegicus (Norway rat)	Col22a1	XP_243609.6
Xenopus tropicalis (tropical clawed frog)	col22a1	XP_004915093.1

Gene Ontology

The following COL22A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL22A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa145800	XM_025147124.1 Latest version!	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa24281	NM_001139439.1 Latest version!	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa145799	XM_025147123.1 Latest version!	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa32913	XM_015283174.1 Latest version!	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL22A1 ( XM_025147124.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002892.1 Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.

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CloneID	OGa145800
Clone ID Related Accession (Same CDS sequence)	XM_025147124.1
Accession Version	XM_025147124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(XXII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGTGA AGAAGGAGCC CCCGGATTTC CTGGACTGAA AGGTGATAAA 
GGAGAAAAAG GGTCCCTTGG CTTGCCTGGC ACCCCAGGGA AGGATGGAGC AAAAGGAGAA 
GCCGGTGAAC CAGGACAACC AGGAGCATTT GGACTGCCTG GGCCAGCAGG TCCAAAGGGA 
TCTGAAGGAA AACAGGGTCC TCCAGGTATT AAAGGCTACG TAGGAGCACC GGGTCTACAA 
GGAGAAAGAG GAGAAAAGGG AACACGAGGA GACAAGGGAG AACGAGGTTT AGATGGGTTC 
CCAGGAAAGC CAGGTGTGGA GGGGAAACAG GGCCCTGCTG GCAGCCCAGG CCCTCCAGGT 
GGCAGTGGGC CCAAGGGAGA AAAGGGTGAA CCAGGGCTCC CTGGATTGCC AGGCTCTTCA 
GTACCTACTG AAGGCCTGAA AGGAGAGCAA GGAGAGCCTG GTCCACGAGG ACCTCAAGGC 
CAACCTGGGC TTCCTGGACT ACCAGGAGAG ACTGGTCCAG AGGGCAAGCC TGGGGTCCCA 
GGTTTACCAG GTCAAAGGGG TAAGAAGGGA GAACCTGGAT TCCCAGGAAT TAATGGTCTG 
ATTGGCCCTC AGGGACCTCC AGGGCCCCCA GGCATTGCTG GACCGCCTGG ACCACCTGGA 
ACACCAGGAC TGCCAGGAGA AATTGGTGTT GCTGGTAAAA GTGGACCGCC AGGACCAGCT 
GGCTCACCTG GCAAAGATGG ATTAAATGGT CTTCCCGGGC TCCCAGGTCA AAAGGGAGAA 
CAAGGAGAAA AGGGTGAGGA AGGCTCTCCT GGAAAAGCTG GCCCTAAGGG TGAACCAGGA 
AGCCGTGGCC AACCTGGTGA ACAGGGTGAA GTGGGTCTTC CTGGTCTTCA GGGTTTACCT 
GGACTAAGAG GAGAAAAAGG AGACCAGGGA GGAAAGGGAA AAGATGGTCT CCCAGGACTG 
AAAGGGGAAA GAGGAGAAAA GGGTGACACA GGTTCCCCTG GCCCACCAGG CATACCTGGA 
ACAACAGCCT TCTTCACCCC ACACCCGAGG ATTCCTGGTG AACCAGGACC AAAGGGAGAG 
AAAGGAGATC GAGGAACAAG TGGAGAACCT GGATTACCAG GGATCCCAGG TGAACAGGGT 
GTCCCAGGGC CTGTAGGACT ACAGGGGACA AAGGGGCAGA AAGGAGCTCA AGGAGAAGCT 
GGAGCCCCTG GAGAGGCTGG TGCAGTTGGA CCAGTGGGGC CACCAGGACC AAGAGGATTG 
CCAGGCAATG TGGGAGTGCC TGGAAACCCT GGTCTACCAG GGAAGGAGGG AGAAAGAGGG 
GAAAAGGGTG ACCCAGGAAA AGAAGGAAAA GTGGGTTTAC CTGGGCCAGC TGGTGTGCCA 
GGGCCTCCTG GACCACCGGG TTTGCCTGGC AAAGGTAAAG ATGGAGAACA GGGGGAAAGA 
GGCCCACCAG GCTTGCCAGG ACCTTTTGGA CATAAGGGTG AGAGGGGAGC TCCTGGCCTC 
CCTGGACAAC CAGGAGACAG AGGTGTGCCA GGAGTTGGAC TGGCTGGACC ACCTGGCCCT 
GTTGGACCCC CAGGAGACAA AGGATCATTG GGTCCTCGTG GTGTACCTGG TATCCCTGGA 
CCACAGGGGC CTCCTGGCCA GGATGGTCTA CCTGGAAATC CAGGGGAAAG AGGACCTCCT 
GGAAAACCAG GTACCTCACC CCTGCTATCT CCAGAGGACA TAAATCTCTT AGTGAAGGAT 
GTGTGCACTG AATGCCCACC TGGCCCACCA GGACTGCCAG GCATCCCAGG TTTCAAAGGT 
GACAAAGGTC TCCGAGGACC ACCAGGTAGA GACGGCCAGG ATGGGAAAAC GGGAAATGCT 
GGTCCCAGAG GTCCTGCAGG CCCACCTGGG CTGGATGGCC AAAAGGGTGC TAAAGGAGAC 
CGTGGTATAA CTGGGGATGC AGGAGAAAAA GGTGAACAGG GACACCCTGG AATGCCTGGC 
TTTGCAGGGG CTCCTGGAAG CCCTGGCCCA CCTGGACCGG ATGGGGCACC GGGACCAATA 
GGACCAGCAG GAAACCCAGG AGACATGGGT AAAGATGGCC CTCCAGGTCC ACAGGGCCCA 
CCAGGTATTC CTGGACTTCC TGGCATTGCT GGTAATGATG GCAAAGATGG CAAGCCAGGA 
TCTCTTGGAG AACCGGGAAA ACCTGGAGAA CCAGGCCTCC CAGGCCAGGA AGGTGCCAGA 
GGCTTACCGG GTTTCAAAGG ACAGAGGGGA GATACAGGTC CCCCAGGCCC ACGGGGGGAA 
CCAGGTGCGA CAGGTCCTGC TGGGCGTGGG GGGCCACCAG GGAAGGATGG TGACACTGGC 
CCCATAGGAC CACAGGGACC TCGAGGACTA AGAGGGCAGC CGGGCAAGAA TGGATTGCCT 
GGATCTGCAG GAGAACCTGG CCCAGCAGGT AACCCTGGTC CTAAAGGAAA TAAAGGAGAA 
AATGGCAGCC CAGGACTCCC TGGCTTCATA GGACCCCGAG GACCACCTGG TGATCCAGGA 
GAAAAAGGCC CTCCAGGAAA GGAGGGTGCA CCAGGGAAAC CAGGTGAAAC TGGATCCAAA 
GGAGAGAGGG GAGAACCTGG CATCAAAGGT GAAAAAGGCC CTCAAGGACA AAAGGGCCCT 
CCAGGTGAGC CTGGGATACC TGGTCATAAA GGCCATCCAG GACTGATGGG ACCCCATGGA 
CCACCAGGAG ACACTGGGCA AGTTGGGCCT CCTGGTCCCC CAGGACAGCC AGGATTTCCA 
GGACCACGGA ACAGAGATTT GATGCTATCC AGTGATGATT ATGGAGACTT AATGCAACTA 
ATAGAGTTAA AATGTCAAAA GATAATATGT AAGCCTTCAA ATTATGTTCC ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002892.1
CDS	358..4794
Translation	MSVMGRCHLPCLLILFTLMFSEERTEAQRAGCKNVHYDLVFILDASSSVGKEDFEKVRQWVSNLVETFEIGPDKTRVGVVRYSDRPTTEFDLGKYKTCEEIKEAARKIRYYGGNTNTGDALRYINTYSFSKEAGGRLSDRTVKKVAILLTDGRSQDYVLDPANAARQAGIRIFAVGVGEALKEELDEIASEPKSAHVFHVSDYNAIDKIRGKLRRRLCENVLCPNVHVEGDRFKHTNGETDEIPGFDLMDSFSVKQIFGMKENGIQSSYVRLGSFPVVQKTEDVFPQGLPDEYAFVTTFKFRKASRKEDWYIWQIIDKYGIPQVSIRLDGENKALEYNAIGLTKDAVRVVFRSARVSDLFDRSWHKIALSIQSAAVSLYIDCKLVQMLPIEDRENIDIQGKTVIGKRLYDSVPIDFDLQRMVIYCDSRHAELETCCDIPSGPCQVTVLTEAPPVEVRPTVGSEQVAHLKTVNCSCPAGQKGERGSIGPEGPKGQKGESGSQGLPGPRGEKGESGRAYSRGEKGEKGEEGAPGFPGLKGDKGEKGSLGLPGTPGKDGAKGEAGEPGQPGAFGLPGPAGPKGSEGKQGPPGIKGYVGAPGLQGERGEKGTRGDKGERGLDGFPGKPGVEGKQGPAGSPGPPGGSGPKGEKGEPGLPGLPGSSVPTEGLKGEQGEPGPRGPQGQPGLPGLPGETGPEGKPGVPGLPGQRGKKGEPGFPGINGLIGPQGPPGPPGIAGPPGPPGTPGLPGEIGVAGKSGPPGPAGSPGKDGLNGLPGLPGQKGEQGEKGEEGSPGKAGPKGEPGSRGQPGEQGEVGLPGLQGLPGLRGEKGDQGGKGKDGLPGLKGERGEKGDTGSPGPPGIPGTTAFFTPHPRIPGEPGPKGEKGDRGTSGEPGLPGIPGEQGVPGPVGLQGTKGQKGAQGEAGAPGEAGAVGPVGPPGPRGLPGNVGVPGNPGLPGKEGERGEKGDPGKEGKVGLPGPAGVPGPPGPPGLPGKGKDGEQGERGPPGLPGPFGHKGERGAPGLPGQPGDRGVPGVGLAGPPGPVGPPGDKGSLGPRGVPGIPGPQGPPGQDGLPGNPGERGPPGKPGTSPLLSPEDINLLVKDVCTECPPGPPGLPGIPGFKGDKGLRGPPGRDGQDGKTGNAGPRGPAGPPGLDGQKGAKGDRGITGDAGEKGEQGHPGMPGFAGAPGSPGPPGPDGAPGPIGPAGNPGDMGKDGPPGPQGPPGIPGLPGIAGNDGKDGKPGSLGEPGKPGEPGLPGQEGARGLPGFKGQRGDTGPPGPRGEPGATGPAGRGGPPGKDGDTGPIGPQGPRGLRGQPGKNGLPGSAGEPGPAGNPGPKGNKGENGSPGLPGFIGPRGPPGDPGEKGPPGKEGAPGKPGETGSKGERGEPGIKGEKGPQGQKGPPGEPGIPGHKGHPGLMGPHGPPGDTGQVGPPGPPGQPGFPGPRNRDLMLSSDDYGDLMQLIELKCQKIICKPSNYVPQ

Target ORF information:

RefSeq Version	XM_025147124.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147124.1

ORF Insert Sequence:

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGTGA AGAAGGAGCC CCCGGATTTC CTGGACTGAA AGGTGATAAA 
GGAGAAAAAG GGTCCCTTGG CTTGCCTGGC ACCCCAGGGA AGGATGGAGC AAAAGGAGAA 
GCCGGTGAAC CAGGACAACC AGGAGCATTT GGACTGCCTG GGCCAGCAGG TCCAAAGGGA 
TCTGAAGGAA AACAGGGTCC TCCAGGTATT AAAGGCTACG TAGGAGCACC GGGTCTACAA 
GGAGAAAGAG GAGAAAAGGG AACACGAGGA GACAAGGGAG AACGAGGTTT AGATGGGTTC 
CCAGGAAAGC CAGGTGTGGA GGGGAAACAG GGCCCTGCTG GCAGCCCAGG CCCTCCAGGT 
GGCAGTGGGC CCAAGGGAGA AAAGGGTGAA CCAGGGCTCC CTGGATTGCC AGGCTCTTCA 
GTACCTACTG AAGGCCTGAA AGGAGAGCAA GGAGAGCCTG GTCCACGAGG ACCTCAAGGC 
CAACCTGGGC TTCCTGGACT ACCAGGAGAG ACTGGTCCAG AGGGCAAGCC TGGGGTCCCA 
GGTTTACCAG GTCAAAGGGG TAAGAAGGGA GAACCTGGAT TCCCAGGAAT TAATGGTCTG 
ATTGGCCCTC AGGGACCTCC AGGGCCCCCA GGCATTGCTG GACCGCCTGG ACCACCTGGA 
ACACCAGGAC TGCCAGGAGA AATTGGTGTT GCTGGTAAAA GTGGACCGCC AGGACCAGCT 
GGCTCACCTG GCAAAGATGG ATTAAATGGT CTTCCCGGGC TCCCAGGTCA AAAGGGAGAA 
CAAGGAGAAA AGGGTGAGGA AGGCTCTCCT GGAAAAGCTG GCCCTAAGGG TGAACCAGGA 
AGCCGTGGCC AACCTGGTGA ACAGGGTGAA GTGGGTCTTC CTGGTCTTCA GGGTTTACCT 
GGACTAAGAG GAGAAAAAGG AGACCAGGGA GGAAAGGGAA AAGATGGTCT CCCAGGACTG 
AAAGGGGAAA GAGGAGAAAA GGGTGACACA GGTTCCCCTG GCCCACCAGG CATACCTGGA 
ACAACAGCCT TCTTCACCCC ACACCCGAGG ATTCCTGGTG AACCAGGACC AAAGGGAGAG 
AAAGGAGATC GAGGAACAAG TGGAGAACCT GGATTACCAG GGATCCCAGG TGAACAGGGT 
GTCCCAGGGC CTGTAGGACT ACAGGGGACA AAGGGGCAGA AAGGAGCTCA AGGAGAAGCT 
GGAGCCCCTG GAGAGGCTGG TGCAGTTGGA CCAGTGGGGC CACCAGGACC AAGAGGATTG 
CCAGGCAATG TGGGAGTGCC TGGAAACCCT GGTCTACCAG GGAAGGAGGG AGAAAGAGGG 
GAAAAGGGTG ACCCAGGAAA AGAAGGAAAA GTGGGTTTAC CTGGGCCAGC TGGTGTGCCA 
GGGCCTCCTG GACCACCGGG TTTGCCTGGC AAAGGTAAAG ATGGAGAACA GGGGGAAAGA 
GGCCCACCAG GCTTGCCAGG ACCTTTTGGA CATAAGGGTG AGAGGGGAGC TCCTGGCCTC 
CCTGGACAAC CAGGAGACAG AGGTGTGCCA GGAGTTGGAC TGGCTGGACC ACCTGGCCCT 
GTTGGACCCC CAGGAGACAA AGGATCATTG GGTCCTCGTG GTGTACCTGG TATCCCTGGA 
CCACAGGGGC CTCCTGGCCA GGATGGTCTA CCTGGAAATC CAGGGGAAAG AGGACCTCCT 
GGAAAACCAG GTACCTCACC CCTGCTATCT CCAGAGGACA TAAATCTCTT AGTGAAGGAT 
GTGTGCACTG AATGCCCACC TGGCCCACCA GGACTGCCAG GCATCCCAGG TTTCAAAGGT 
GACAAAGGTC TCCGAGGACC ACCAGGTAGA GACGGCCAGG ATGGGAAAAC GGGAAATGCT 
GGTCCCAGAG GTCCTGCAGG CCCACCTGGG CTGGATGGCC AAAAGGGTGC TAAAGGAGAC 
CGTGGTATAA CTGGGGATGC AGGAGAAAAA GGTGAACAGG GACACCCTGG AATGCCTGGC 
TTTGCAGGGG CTCCTGGAAG CCCTGGCCCA CCTGGACCGG ATGGGGCACC GGGACCAATA 
GGACCAGCAG GAAACCCAGG AGACATGGGT AAAGATGGCC CTCCAGGTCC ACAGGGCCCA 
CCAGGTATTC CTGGACTTCC TGGCATTGCT GGTAATGATG GCAAAGATGG CAAGCCAGGA 
TCTCTTGGAG AACCGGGAAA ACCTGGAGAA CCAGGCCTCC CAGGCCAGGA AGGTGCCAGA 
GGCTTACCGG GTTTCAAAGG ACAGAGGGGA GATACAGGTC CCCCAGGCCC ACGGGGGGAA 
CCAGGTGCGA CAGGTCCTGC TGGGCGTGGG GGGCCACCAG GGAAGGATGG TGACACTGGC 
CCCATAGGAC CACAGGGACC TCGAGGACTA AGAGGGCAGC CGGGCAAGAA TGGATTGCCT 
GGATCTGCAG GAGAACCTGG CCCAGCAGGT AACCCTGGTC CTAAAGGAAA TAAAGGAGAA 
AATGGCAGCC CAGGACTCCC TGGCTTCATA GGACCCCGAG GACCACCTGG TGATCCAGGA 
GAAAAAGGCC CTCCAGGAAA GGAGGGTGCA CCAGGGAAAC CAGGTGAAAC TGGATCCAAA 
GGAGAGAGGG GAGAACCTGG CATCAAAGGT GAAAAAGGCC CTCAAGGACA AAAGGGCCCT 
CCAGGTGAGC CTGGGATACC TGGTCATAAA GGCCATCCAG GACTGATGGG ACCCCATGGA 
CCACCAGGAG ACACTGGGCA AGTTGGGCCT CCTGGTCCCC CAGGACAGCC AGGATTTCCA 
GGACCACGGA ACAGAGATTT GATGCTATCC AGTGATGATT ATGGAGACTT AATGCAACTA 
ATAGAGTTAA AATGTCAAAA GATAATATGT AAGCCTTCAA ATTATGTTCC ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( NM_001139439.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001132911.1 Gallus gallus collagen type XXII alpha 1 chain (COL22A1), mRNA.

Price & Availability↑

CloneID	OGa24281
Clone ID Related Accession (Same CDS sequence)	NM_001139439.1
Accession Version	NM_001139439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548086400000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(XXII) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000664.1. On Nov 15, 2008 this sequence version replaced XR_026844.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201380, SAMN05341171 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGGTC CCTTGGCTTG CCTGGCACCC CAGGGAAGGA TGGAGCAAAA 
GGAGAAGCCG GTGAACCAGG ACAACCAGGA GCATTTGGAC TGCCTGGGCC AGCAGGTCCA 
AAGGGATCTG AAGGAAAACA GGGTCCTCCA GGTATTAAAG GCTACGTAGG AGCACCGGGT 
CTACAAGGAG AAAGAGGAGA AAAGGGAACA CGAGGAGACA AGGGAGAACG AGGTTTAGAT 
GGGTTCCCAG GAAAGCCAGG TGTGGAGGGG AAACAGGGCC CTGCTGGCAG CCCAGGCCCT 
CCAGGTGGCA GTGGGCCCAA GGGAGAAAAG GGCAGTACGG GTGAACCAGG GCTCCCTGGA 
TTGCCAGGCT CTTCAGTACC TACTGAAGGC CTGAAAGGAG AGCAAGGAGA GCCTGGTCCA 
CGAGGACCTC AAGGCCAACC TGGGCTTCCT GGACTACCAG GAGAGACTGG TCCAGAGGGC 
AAGCCTGGGG TCCCAGGTTT ACCAGGTCAA AGGGGTAAGA AGGGAGAACC TGGATTCCCA 
GGAATTAATG GTCTGATTGG CCCTCAGGGA CCTCCAGGGC CCCCAGGCAT TGCTGGACCG 
CCTGGACCAC CTGGAACACC AGGACTGCCA GGAGAAATTG GTGTTGCTGG TAAAAGTGGA 
CCGCCAGGAC CAGCTGGCTC ACCTGGCAAA GATGGATTAA ATGGTCTTCC CGGGCTCCCA 
GGTCAAAAGG GAGAACAAGG AGAAAAGGGT GAGGAAGGCT CTCCTGGAAA AGCTGGCCCT 
AAGGGTGAAC CAGGAAGCCG TGGCCAACCT GGTGAACAGG GTGAAGTGGG TCTTCCTGGT 
CTTCAGGGTT TACCTGGACT AAGAGGAGAA AAAGGAGACC AGGGAGGAAA GGGAAAAGAT 
GGTCTCCCAG GACTGAAAGG GGAAAGAGGA GAAAAGGGTG ACACAGGTTC CCCTGGCCCA 
CCAGGCATAC CTGGAACAAC AGCCTTCTTC ACCCCACACC CGAGGATTCC TGGTGAACCA 
GGACCAAAGG GAGAGAAAGG AGATCGAGGA ACAAGTGGAG AACCTGGATT ACCAGGGATC 
CCAGGTGAAC AGGGTGTCCC AGGGCCTGTA GGACTACAGG GGACAAAGGG GCAGAAAGGA 
GCTCAAGGAG AAGCTGGAGC CCCTGGAGAG GCTGGTGCAG TTGGACCAGT GGGGCCACCA 
GGACCAAGAG GATTGCCAGG CAATGTGGGA GTGCCTGGAA ACCCTGGTCT ACCAGGGAAG 
GAGGGAGAAA GAGGGGAAAA GGGTGACCCA GGAAAAGAAG GAAAAGTGGG TTTACCTGGG 
CCAGCTGGTG TGCCAGGGCC TCCTGGACCA CCGGGTTTGC CTGGCAAAGG TAAAGATGGA 
GAACAGGGGG AAAGAGGCCC ACCAGGCTTG CCAGGACCTT TTGGACATAA GGGTGAGAGG 
GGAGCTCCTG GCCTCCCTGG ACAACCAGGA GACAGAGGTG TGCCAGGAGT TGGACTGGCT 
GGACCACCTG GCCCTGTTGG ACCCCCAGGA GACAAAGGAT CATTGGGTCC TCGTGGTGTA 
CCTGGTATCC CTGGACCACA GGGGCCTCCT GGCCAGGATG GTCTACCTGG AAATCCAGGG 
GAAAGAGGAC CTCCTGGAAA ACCAGGTACC TCACCCCTGC TATCTCCAGA GGACATAAAT 
CTCTTAGTGA AGGATGTGTG CACTGAATGC CCACCTGGCC CACCAGGACT GCCAGGCATC 
CCAGGTTTCA AAGGTGACAA AGGTCTCCGA GGACCACCAG GTAGAGACGG CCAGGATGGG 
AAAACGGGAA ATGCTGGTCC CAGAGGTCCT GCAGGCCCAC CTGGGCTGGA TGGCCAAAAG 
GGTGCTAAAG GAGACCGTGG TATAACTGGG GATGCAGGAG AAAAAGGTGA ACAGGGACAC 
CCTGGAATGC CTGGCTTTGC AGGGGCTCCT GGAAGCCCTG GCCCACCTGG ACCGGATGGG 
GCACCGGGAC CAATAGGACC AGCAGGAAAC CCAGGAGACA TGGGTAAAGA TGGCCCTCCA 
GGTCCACAGG GCCCACCAGG TATTCCTGGA CTTCCTGGCA TTGCTGGTAA TGATGGCAAA 
GATGGCAAGC CAGGATCTCT TGGAGAACCG GGAAAACCTG GAGAACCAGG CCTCCCAGGC 
CAGGAAGGTG CCAGAGGCTT ACCGGGTTTC AAAGGACAGA GGGGAGATAC AGGTCCCCCA 
GGCCCACGGG GGGAACCAGG TGCGACAGGT CCTGCTGGGC GTGGGGGGCC ACCAGGGAAG 
GATGGTGACA CTGGCCCCAT AGGACCACAG GGACCTCGAG GACTAAGAGG GCAGCCGGGC 
AAGAATGGAT TGCCTGGATC TGCAGGAGAA CCTGGCCCAG CAGGTAACCC TGGTCCTAAA 
GGAAATAAAG GAGAAAATGG CAGCCCAGGA CTCCCTGGCT TCATAGGACC CCGAGGACCA 
CCTGGTGATC CAGGAGAAAA AGGCCCTCCA GGAAAGGAGG GTGCACCAGG GAAACCAGGT 
GAAACTGGAT CCAAAGGAGA GAGGGGAGAA CCTGGCATCA AAGGTGAAAA AGGCCCTCAA 
GGACAAAAGG GCCCTCCAGG TGAGCCTGGG ATACCTGGTC ATAAAGGCCA TCCAGGACTG 
ATGGGACCCC ATGGACCACC AGGAGACACT GGGCAAGTTG GGCCTCCTGG TCCCCCAGGA 
CAGCCAGGAT TTCCAGGACC ACGGGGGGAA CCTCCGTCTC TAGAGACTCT GAGAAGACTC 
ATCCAAGAGG AGTTAGCTAA GCAGCTAGAT GCAAAGTTAG CCTATCTCTT GGCTCAGATA 
CCGCCTGCAC ATGTGAAAGC ATCCCATGGC AGACCAGGAC CTCCTGGTCC CCCTGGGAAA 
GAAGGACTAC CAGGAAGAAC TGGCCCTCCA GGAGAGCCTG GCCGCCCTGG ACAGACTGGA 
TCTGAAGGAC CACCCGGACC CATTGGTCCC AAGGGAGAAA GAGGAGCGAA GGGTGAGAAA 
GGAGATGCTG GCATTGGCCA GCGAGGTGAA ATAGGTCCTC CAGGAATTCC AGGCCTCCCA 
GGGGAGCCTG GCTATGCCAA AGATGGGATA CCAGGACCAC CTGGCCCTCA AGGTGAAGCT 
GGGGTACCTG GTCTCATGGG CCCACAGGGA CCTCCGGGAG CCAATGGACA GTGTGACCTC 
GCTCAGTGCG CTTACTATGC AAGCCTGGCT GCCAGGCCCG CCAATGTCAA AGGGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001132911.1
CDS	279..5078
Translation	MSVMGRCHLPCLLILFTLMFSEERTEAQRAGCKNVHYDLVFILDASSSVGKEDFEKVRQWVSNLVETFEIGPDKTRVGVVRYSDRPTTEFDLGKYKTCEEIKEAARKIRYYGGNTNTGDALRYINTYSFSKEAGGRLSDRTVKKVAILLTDGRSQDYVLDPANAARQAGIRIFAVGVGEALKEELDEIASEPKSAHVFHVSDYNAIDKIRGKLRRRLCENVLCPNVHVEGDRFKHTNGETDEIPGFDLMDSFSVKQIFGMKENGIQSSYVRLGSFPVVQKTEDVFPQGLPDEYAFVTTFKFRKASRKEDWYIWQIIDKYGIPQVSIRLDGENKALEYNAIGLTKDAVRVVFRSARVSDLFDRSWHKIALSIQSAAVSLYIDCKLVQMLPIEDRENIDIQGKTVIGKRLYDSVPIDFDLQRMVIYCDSRHAELETCCDIPSGPCQVTVLTEAPPVEVRPTVGSEQVAHLKTVNCSCPAGQKGERGSIGPEGPKGQKGESGSQGLPGPRGEKGESGRAYSRGEKGEKGSLGLPGTPGKDGAKGEAGEPGQPGAFGLPGPAGPKGSEGKQGPPGIKGYVGAPGLQGERGEKGTRGDKGERGLDGFPGKPGVEGKQGPAGSPGPPGGSGPKGEKGSTGEPGLPGLPGSSVPTEGLKGEQGEPGPRGPQGQPGLPGLPGETGPEGKPGVPGLPGQRGKKGEPGFPGINGLIGPQGPPGPPGIAGPPGPPGTPGLPGEIGVAGKSGPPGPAGSPGKDGLNGLPGLPGQKGEQGEKGEEGSPGKAGPKGEPGSRGQPGEQGEVGLPGLQGLPGLRGEKGDQGGKGKDGLPGLKGERGEKGDTGSPGPPGIPGTTAFFTPHPRIPGEPGPKGEKGDRGTSGEPGLPGIPGEQGVPGPVGLQGTKGQKGAQGEAGAPGEAGAVGPVGPPGPRGLPGNVGVPGNPGLPGKEGERGEKGDPGKEGKVGLPGPAGVPGPPGPPGLPGKGKDGEQGERGPPGLPGPFGHKGERGAPGLPGQPGDRGVPGVGLAGPPGPVGPPGDKGSLGPRGVPGIPGPQGPPGQDGLPGNPGERGPPGKPGTSPLLSPEDINLLVKDVCTECPPGPPGLPGIPGFKGDKGLRGPPGRDGQDGKTGNAGPRGPAGPPGLDGQKGAKGDRGITGDAGEKGEQGHPGMPGFAGAPGSPGPPGPDGAPGPIGPAGNPGDMGKDGPPGPQGPPGIPGLPGIAGNDGKDGKPGSLGEPGKPGEPGLPGQEGARGLPGFKGQRGDTGPPGPRGEPGATGPAGRGGPPGKDGDTGPIGPQGPRGLRGQPGKNGLPGSAGEPGPAGNPGPKGNKGENGSPGLPGFIGPRGPPGDPGEKGPPGKEGAPGKPGETGSKGERGEPGIKGEKGPQGQKGPPGEPGIPGHKGHPGLMGPHGPPGDTGQVGPPGPPGQPGFPGPRGEPPSLETLRRLIQEELAKQLDAKLAYLLAQIPPAHVKASHGRPGPPGPPGKEGLPGRTGPPGEPGRPGQTGSEGPPGPIGPKGERGAKGEKGDAGIGQRGEIGPPGIPGLPGEPGYAKDGIPGPPGPQGEAGVPGLMGPQGPPGANGQCDLAQCAYYASLAARPANVKGP

Target ORF information:

RefSeq Version	NM_001139439.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001139439.1

ORF Insert Sequence:

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGGTC CCTTGGCTTG CCTGGCACCC CAGGGAAGGA TGGAGCAAAA 
GGAGAAGCCG GTGAACCAGG ACAACCAGGA GCATTTGGAC TGCCTGGGCC AGCAGGTCCA 
AAGGGATCTG AAGGAAAACA GGGTCCTCCA GGTATTAAAG GCTACGTAGG AGCACCGGGT 
CTACAAGGAG AAAGAGGAGA AAAGGGAACA CGAGGAGACA AGGGAGAACG AGGTTTAGAT 
GGGTTCCCAG GAAAGCCAGG TGTGGAGGGG AAACAGGGCC CTGCTGGCAG CCCAGGCCCT 
CCAGGTGGCA GTGGGCCCAA GGGAGAAAAG GGCAGTACGG GTGAACCAGG GCTCCCTGGA 
TTGCCAGGCT CTTCAGTACC TACTGAAGGC CTGAAAGGAG AGCAAGGAGA GCCTGGTCCA 
CGAGGACCTC AAGGCCAACC TGGGCTTCCT GGACTACCAG GAGAGACTGG TCCAGAGGGC 
AAGCCTGGGG TCCCAGGTTT ACCAGGTCAA AGGGGTAAGA AGGGAGAACC TGGATTCCCA 
GGAATTAATG GTCTGATTGG CCCTCAGGGA CCTCCAGGGC CCCCAGGCAT TGCTGGACCG 
CCTGGACCAC CTGGAACACC AGGACTGCCA GGAGAAATTG GTGTTGCTGG TAAAAGTGGA 
CCGCCAGGAC CAGCTGGCTC ACCTGGCAAA GATGGATTAA ATGGTCTTCC CGGGCTCCCA 
GGTCAAAAGG GAGAACAAGG AGAAAAGGGT GAGGAAGGCT CTCCTGGAAA AGCTGGCCCT 
AAGGGTGAAC CAGGAAGCCG TGGCCAACCT GGTGAACAGG GTGAAGTGGG TCTTCCTGGT 
CTTCAGGGTT TACCTGGACT AAGAGGAGAA AAAGGAGACC AGGGAGGAAA GGGAAAAGAT 
GGTCTCCCAG GACTGAAAGG GGAAAGAGGA GAAAAGGGTG ACACAGGTTC CCCTGGCCCA 
CCAGGCATAC CTGGAACAAC AGCCTTCTTC ACCCCACACC CGAGGATTCC TGGTGAACCA 
GGACCAAAGG GAGAGAAAGG AGATCGAGGA ACAAGTGGAG AACCTGGATT ACCAGGGATC 
CCAGGTGAAC AGGGTGTCCC AGGGCCTGTA GGACTACAGG GGACAAAGGG GCAGAAAGGA 
GCTCAAGGAG AAGCTGGAGC CCCTGGAGAG GCTGGTGCAG TTGGACCAGT GGGGCCACCA 
GGACCAAGAG GATTGCCAGG CAATGTGGGA GTGCCTGGAA ACCCTGGTCT ACCAGGGAAG 
GAGGGAGAAA GAGGGGAAAA GGGTGACCCA GGAAAAGAAG GAAAAGTGGG TTTACCTGGG 
CCAGCTGGTG TGCCAGGGCC TCCTGGACCA CCGGGTTTGC CTGGCAAAGG TAAAGATGGA 
GAACAGGGGG AAAGAGGCCC ACCAGGCTTG CCAGGACCTT TTGGACATAA GGGTGAGAGG 
GGAGCTCCTG GCCTCCCTGG ACAACCAGGA GACAGAGGTG TGCCAGGAGT TGGACTGGCT 
GGACCACCTG GCCCTGTTGG ACCCCCAGGA GACAAAGGAT CATTGGGTCC TCGTGGTGTA 
CCTGGTATCC CTGGACCACA GGGGCCTCCT GGCCAGGATG GTCTACCTGG AAATCCAGGG 
GAAAGAGGAC CTCCTGGAAA ACCAGGTACC TCACCCCTGC TATCTCCAGA GGACATAAAT 
CTCTTAGTGA AGGATGTGTG CACTGAATGC CCACCTGGCC CACCAGGACT GCCAGGCATC 
CCAGGTTTCA AAGGTGACAA AGGTCTCCGA GGACCACCAG GTAGAGACGG CCAGGATGGG 
AAAACGGGAA ATGCTGGTCC CAGAGGTCCT GCAGGCCCAC CTGGGCTGGA TGGCCAAAAG 
GGTGCTAAAG GAGACCGTGG TATAACTGGG GATGCAGGAG AAAAAGGTGA ACAGGGACAC 
CCTGGAATGC CTGGCTTTGC AGGGGCTCCT GGAAGCCCTG GCCCACCTGG ACCGGATGGG 
GCACCGGGAC CAATAGGACC AGCAGGAAAC CCAGGAGACA TGGGTAAAGA TGGCCCTCCA 
GGTCCACAGG GCCCACCAGG TATTCCTGGA CTTCCTGGCA TTGCTGGTAA TGATGGCAAA 
GATGGCAAGC CAGGATCTCT TGGAGAACCG GGAAAACCTG GAGAACCAGG CCTCCCAGGC 
CAGGAAGGTG CCAGAGGCTT ACCGGGTTTC AAAGGACAGA GGGGAGATAC AGGTCCCCCA 
GGCCCACGGG GGGAACCAGG TGCGACAGGT CCTGCTGGGC GTGGGGGGCC ACCAGGGAAG 
GATGGTGACA CTGGCCCCAT AGGACCACAG GGACCTCGAG GACTAAGAGG GCAGCCGGGC 
AAGAATGGAT TGCCTGGATC TGCAGGAGAA CCTGGCCCAG CAGGTAACCC TGGTCCTAAA 
GGAAATAAAG GAGAAAATGG CAGCCCAGGA CTCCCTGGCT TCATAGGACC CCGAGGACCA 
CCTGGTGATC CAGGAGAAAA AGGCCCTCCA GGAAAGGAGG GTGCACCAGG GAAACCAGGT 
GAAACTGGAT CCAAAGGAGA GAGGGGAGAA CCTGGCATCA AAGGTGAAAA AGGCCCTCAA 
GGACAAAAGG GCCCTCCAGG TGAGCCTGGG ATACCTGGTC ATAAAGGCCA TCCAGGACTG 
ATGGGACCCC ATGGACCACC AGGAGACACT GGGCAAGTTG GGCCTCCTGG TCCCCCAGGA 
CAGCCAGGAT TTCCAGGACC ACGGGGGGAA CCTCCGTCTC TAGAGACTCT GAGAAGACTC 
ATCCAAGAGG AGTTAGCTAA GCAGCTAGAT GCAAAGTTAG CCTATCTCTT GGCTCAGATA 
CCGCCTGCAC ATGTGAAAGC ATCCCATGGC AGACCAGGAC CTCCTGGTCC CCCTGGGAAA 
GAAGGACTAC CAGGAAGAAC TGGCCCTCCA GGAGAGCCTG GCCGCCCTGG ACAGACTGGA 
TCTGAAGGAC CACCCGGACC CATTGGTCCC AAGGGAGAAA GAGGAGCGAA GGGTGAGAAA 
GGAGATGCTG GCATTGGCCA GCGAGGTGAA ATAGGTCCTC CAGGAATTCC AGGCCTCCCA 
GGGGAGCCTG GCTATGCCAA AGATGGGATA CCAGGACCAC CTGGCCCTCA AGGTGAAGCT 
GGGGTACCTG GTCTCATGGG CCCACAGGGA CCTCCGGGAG CCAATGGACA GTGTGACCTC 
GCTCAGTGCG CTTACTATGC AAGCCTGGCT GCCAGGCCCG CCAATGTCAA AGGGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_025147123.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025002891.1 Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X2, mRNA.

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CloneID	OGa145799
Clone ID Related Accession (Same CDS sequence)	XM_025147123.1
Accession Version	XM_025147123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(XXII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGTGA AGAAGGAGCC CCCGGATTTC CTGGACTGAA AGGTGATAAA 
GGAGAAAAAG GGTCCCTTGG CTTGCCTGGC ACCCCAGGGA AGGATGGAGC AAAAGGAGAA 
GCCGGTGAAC CAGGACAACC AGGAGCATTT GGACTGCCTG GGCCAGCAGG TCCAAAGGGA 
TCTGAAGGAA AACAGGGTCC TCCAGGTATT AAAGGCTACG TAGGAGCACC GGGTCTACAA 
GGAGAAAGAG GAGAAAAGGG AACACGAGGA GACAAGGGAG AACGAGGTTT AGATGGGTTC 
CCAGGAAAGC CAGGTGTGGA GGGGAAACAG GGCCCTGCTG GCAGCCCAGG CCCTCCAGGT 
GGCAGTGGGC CCAAGGGAGA AAAGGGTGAA CCAGGGCTCC CTGGATTGCC AGGCTCTTCA 
GTACCTACTG AAGGCCTGAA AGGAGAGCAA GGAGAGCCTG GTCCACGAGG ACCTCAAGGC 
CAACCTGGGC TTCCTGGACT ACCAGGAGAG ACTGGTCCAG AGGGCAAGCC TGGGGTCCCA 
GGTTTACCAG GTCAAAGGGG TAAGAAGGGA GAACCTGGAT TCCCAGGAAT TAATGGTCTG 
ATTGGCCCTC AGGGACCTCC AGGGCCCCCA GGCATTGCTG GACCGCCTGG ACCACCTGGA 
ACACCAGGAC TGCCAGGAGA AATTGGTGTT GCTGGTAAAA GTGGACCGCC AGGACCAGCT 
GGCTCACCTG GCAAAGATGG ATTAAATGGT CTTCCCGGGC TCCCAGGTCA AAAGGGAGAA 
CAAGGAGAAA AGGGTGAGGA AGGCTCTCCT GGAAAAGCTG GCCCTAAGGG TGAACCAGGA 
AGCCGTGGCC AACCTGGTGA ACAGGGTGAA GTGGGTCTTC CTGGTCTTCA GGGTTTACCT 
GGACTAAGAG GAGAAAAAGG AGACCAGGGA GGAAAGGGAA AAGATGGTCT CCCAGGACTG 
AAAGGGGAAA GAGGAGAAAA GGGTGACACA GGTTCCCCTG GCCCACCAGG CATACCTGGA 
ACAACAGCCT TCTTCACCCC ACACCCGAGG ATTCCTGGTG AACCAGGACC AAAGGGAGAG 
AAAGGAGATC GAGGAACAAG TGGAGAACCT GGATTACCAG GGATCCCAGG TGAACAGGGT 
GTCCCAGGGC CTGTAGGACT ACAGGGGACA AAGGGGCAGA AAGGAGCTCA AGGAGAAGCT 
GGAGCCCCTG GAGAGGCTGG TGCAGTTGGA CCAGTGGGGC CACCAGGACC AAGAGGATTG 
CCAGGCAATG TGGGAGTGCC TGGAAACCCT GGTCTACCAG GGAAGGAGGG AGAAAGAGGG 
GAAAAGGGTG ACCCAGGAAA AGAAGGAAAA GTGGGTTTAC CTGGGCCAGC TGGTGTGCCA 
GGGCCTCCTG GACCACCGGG TTTGCCTGGC AAAGGTAAAG ATGGAGAACA GGGGGAAAGA 
GGCCCACCAG GCTTGCCAGG ACCTTTTGGA CATAAGGGTG AGAGGGGAGC TCCTGGCCTC 
CCTGGACAAC CAGGAGACAG AGGTGTGCCA GGAGTTGGAC TGGCTGGACC ACCTGGCCCT 
GTTGGACCCC CAGGAGACAA AGGATCATTG GGTCCTCGTG GTGTACCTGG TATCCCTGGA 
CCACAGGGGC CTCCTGGCCA GGATGGTCTA CCTGGAAATC CAGGGGAAAG AGGACCTCCT 
GGAAAACCAG ATGTGTGCAC TGAATGCCCA CCTGGCCCAC CAGGACTGCC AGGCATCCCA 
GGTTTCAAAG GTGACAAAGG TCTCCGAGGA CCACCAGGTA GAGACGGCCA GGATGGGAAA 
ACGGGAAATG CTGGTCCCAG AGGTCCTGCA GGCCCACCTG GGCTGGATGG CCAAAAGGGT 
GCTAAAGGAG ACCGTGGTAT AACTGGGGAT GCAGGAGAAA AAGGTGAACA GGGACACCCT 
GGAATGCCTG GCTTTGCAGG GGCTCCTGGA AGCCCTGGCC CACCTGGACC GGATGGGGCA 
CCGGGACCAA TAGGACCAGC AGGAAACCCA GGAGACATGG GTAAAGATGG CCCTCCAGGT 
CCACAGGGCC CACCAGGTAT TCCTGGACTT CCTGGCATTG CTGGTAATGA TGGCAAAGAT 
GGCAAGCCAG GATCTCTTGG AGAACCGGGA AAACCTGGAG AACCAGGCCT CCCAGGCCAG 
GAAGGTGCCA GAGGCTTACC GGGTTTCAAA GGACAGAGGG GAGATACAGG TCCCCCAGGC 
CCACGGGGGG AACCAGGTGC GACAGGTCCT GCTGGGCGTG GGGGGCCACC AGGGAAGGAT 
GGTGACACTG GCCCCATAGG ACCACAGGGA CCTCGAGGAC TAAGAGGGCA GCCGGGCAAG 
AATGGATTGC CTGGATCTGC AGGAGAACCT GGCCCAGCAG GTAACCCTGG TCCTAAAGGA 
AATAAAGGAG AAAATGGCAG CCCAGGACTC CCTGGCTTCA TAGGACCCCG AGGACCACCT 
GGTGATCCAG GAGAAAAAGG CCCTCCAGGA AAGGAGGGTG CACCAGGGAA ACCAGGTGAA 
ACTGGATCCA AAGGAGAGAG GGGAGAACCT GGCATCAAAG GTGAAAAAGG CCCTCAAGGA 
CAAAAGGGCC CTCCAGGTGA GCCTGGGATA CCTGGTCATA AAGGCCATCC AGGACTGATG 
GGACCCCATG GACCACCAGG AGACACTGGG CAAGTTGGGC CTCCTGGTCC CCCAGGACAG 
CCAGGATTTC CAGGACCACG GGGGGAACCT CCGTCTCTAG AGACTCTGAG AAGACTCATC 
CAAGAGGAGT TAGCTAAGCA GCTAGATGCA AAGTTAGCCT ATCTCTTGGC TCAGATACCG 
CCTGCACATG TGAAAGCATC CCATGGCAGA CCAGGACCTC CTGGTCCCCC TGGGAAAGAA 
GGACTACCAG GAAGAACTGG CCCTCCAGGA GAGCCTGGCC GCCCTGGACA GACTGGATCT 
GAAGGACCAC CCGGACCCAT TGGTCCCAAG GGAGAAAGAG GAGCGAAGGG TGAGAAAGGA 
GATGCTGGCA TTGGCCAGCG AGGTGAAATA GGTCCTCCAG GAATTCCAGG CCTCCCAGGG 
GAGCCTGGCT ATGCCAAAGA TGGGATACCA GGACCACCTG GCCCTCAAGG TGAAGCTGGG 
GTACCTGGTC TCATGGGCCC ACAGGGACCT CCGGGAGCCA ATGGACAGTG TGACCTCGCT 
CAGTGCGCTT ACTATGCAAG CCTGGCTGCC AGGCCCGCCA ATGTCAAAGG GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025002891.1
CDS	358..5154
Translation	MSVMGRCHLPCLLILFTLMFSEERTEAQRAGCKNVHYDLVFILDASSSVGKEDFEKVRQWVSNLVETFEIGPDKTRVGVVRYSDRPTTEFDLGKYKTCEEIKEAARKIRYYGGNTNTGDALRYINTYSFSKEAGGRLSDRTVKKVAILLTDGRSQDYVLDPANAARQAGIRIFAVGVGEALKEELDEIASEPKSAHVFHVSDYNAIDKIRGKLRRRLCENVLCPNVHVEGDRFKHTNGETDEIPGFDLMDSFSVKQIFGMKENGIQSSYVRLGSFPVVQKTEDVFPQGLPDEYAFVTTFKFRKASRKEDWYIWQIIDKYGIPQVSIRLDGENKALEYNAIGLTKDAVRVVFRSARVSDLFDRSWHKIALSIQSAAVSLYIDCKLVQMLPIEDRENIDIQGKTVIGKRLYDSVPIDFDLQRMVIYCDSRHAELETCCDIPSGPCQVTVLTEAPPVEVRPTVGSEQVAHLKTVNCSCPAGQKGERGSIGPEGPKGQKGESGSQGLPGPRGEKGESGRAYSRGEKGEKGEEGAPGFPGLKGDKGEKGSLGLPGTPGKDGAKGEAGEPGQPGAFGLPGPAGPKGSEGKQGPPGIKGYVGAPGLQGERGEKGTRGDKGERGLDGFPGKPGVEGKQGPAGSPGPPGGSGPKGEKGEPGLPGLPGSSVPTEGLKGEQGEPGPRGPQGQPGLPGLPGETGPEGKPGVPGLPGQRGKKGEPGFPGINGLIGPQGPPGPPGIAGPPGPPGTPGLPGEIGVAGKSGPPGPAGSPGKDGLNGLPGLPGQKGEQGEKGEEGSPGKAGPKGEPGSRGQPGEQGEVGLPGLQGLPGLRGEKGDQGGKGKDGLPGLKGERGEKGDTGSPGPPGIPGTTAFFTPHPRIPGEPGPKGEKGDRGTSGEPGLPGIPGEQGVPGPVGLQGTKGQKGAQGEAGAPGEAGAVGPVGPPGPRGLPGNVGVPGNPGLPGKEGERGEKGDPGKEGKVGLPGPAGVPGPPGPPGLPGKGKDGEQGERGPPGLPGPFGHKGERGAPGLPGQPGDRGVPGVGLAGPPGPVGPPGDKGSLGPRGVPGIPGPQGPPGQDGLPGNPGERGPPGKPDVCTECPPGPPGLPGIPGFKGDKGLRGPPGRDGQDGKTGNAGPRGPAGPPGLDGQKGAKGDRGITGDAGEKGEQGHPGMPGFAGAPGSPGPPGPDGAPGPIGPAGNPGDMGKDGPPGPQGPPGIPGLPGIAGNDGKDGKPGSLGEPGKPGEPGLPGQEGARGLPGFKGQRGDTGPPGPRGEPGATGPAGRGGPPGKDGDTGPIGPQGPRGLRGQPGKNGLPGSAGEPGPAGNPGPKGNKGENGSPGLPGFIGPRGPPGDPGEKGPPGKEGAPGKPGETGSKGERGEPGIKGEKGPQGQKGPPGEPGIPGHKGHPGLMGPHGPPGDTGQVGPPGPPGQPGFPGPRGEPPSLETLRRLIQEELAKQLDAKLAYLLAQIPPAHVKASHGRPGPPGPPGKEGLPGRTGPPGEPGRPGQTGSEGPPGPIGPKGERGAKGEKGDAGIGQRGEIGPPGIPGLPGEPGYAKDGIPGPPGPQGEAGVPGLMGPQGPPGANGQCDLAQCAYYASLAARPANVKGP

Target ORF information:

RefSeq Version	XM_025147123.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025147123.1

ORF Insert Sequence:

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGTGA AGAAGGAGCC CCCGGATTTC CTGGACTGAA AGGTGATAAA 
GGAGAAAAAG GGTCCCTTGG CTTGCCTGGC ACCCCAGGGA AGGATGGAGC AAAAGGAGAA 
GCCGGTGAAC CAGGACAACC AGGAGCATTT GGACTGCCTG GGCCAGCAGG TCCAAAGGGA 
TCTGAAGGAA AACAGGGTCC TCCAGGTATT AAAGGCTACG TAGGAGCACC GGGTCTACAA 
GGAGAAAGAG GAGAAAAGGG AACACGAGGA GACAAGGGAG AACGAGGTTT AGATGGGTTC 
CCAGGAAAGC CAGGTGTGGA GGGGAAACAG GGCCCTGCTG GCAGCCCAGG CCCTCCAGGT 
GGCAGTGGGC CCAAGGGAGA AAAGGGTGAA CCAGGGCTCC CTGGATTGCC AGGCTCTTCA 
GTACCTACTG AAGGCCTGAA AGGAGAGCAA GGAGAGCCTG GTCCACGAGG ACCTCAAGGC 
CAACCTGGGC TTCCTGGACT ACCAGGAGAG ACTGGTCCAG AGGGCAAGCC TGGGGTCCCA 
GGTTTACCAG GTCAAAGGGG TAAGAAGGGA GAACCTGGAT TCCCAGGAAT TAATGGTCTG 
ATTGGCCCTC AGGGACCTCC AGGGCCCCCA GGCATTGCTG GACCGCCTGG ACCACCTGGA 
ACACCAGGAC TGCCAGGAGA AATTGGTGTT GCTGGTAAAA GTGGACCGCC AGGACCAGCT 
GGCTCACCTG GCAAAGATGG ATTAAATGGT CTTCCCGGGC TCCCAGGTCA AAAGGGAGAA 
CAAGGAGAAA AGGGTGAGGA AGGCTCTCCT GGAAAAGCTG GCCCTAAGGG TGAACCAGGA 
AGCCGTGGCC AACCTGGTGA ACAGGGTGAA GTGGGTCTTC CTGGTCTTCA GGGTTTACCT 
GGACTAAGAG GAGAAAAAGG AGACCAGGGA GGAAAGGGAA AAGATGGTCT CCCAGGACTG 
AAAGGGGAAA GAGGAGAAAA GGGTGACACA GGTTCCCCTG GCCCACCAGG CATACCTGGA 
ACAACAGCCT TCTTCACCCC ACACCCGAGG ATTCCTGGTG AACCAGGACC AAAGGGAGAG 
AAAGGAGATC GAGGAACAAG TGGAGAACCT GGATTACCAG GGATCCCAGG TGAACAGGGT 
GTCCCAGGGC CTGTAGGACT ACAGGGGACA AAGGGGCAGA AAGGAGCTCA AGGAGAAGCT 
GGAGCCCCTG GAGAGGCTGG TGCAGTTGGA CCAGTGGGGC CACCAGGACC AAGAGGATTG 
CCAGGCAATG TGGGAGTGCC TGGAAACCCT GGTCTACCAG GGAAGGAGGG AGAAAGAGGG 
GAAAAGGGTG ACCCAGGAAA AGAAGGAAAA GTGGGTTTAC CTGGGCCAGC TGGTGTGCCA 
GGGCCTCCTG GACCACCGGG TTTGCCTGGC AAAGGTAAAG ATGGAGAACA GGGGGAAAGA 
GGCCCACCAG GCTTGCCAGG ACCTTTTGGA CATAAGGGTG AGAGGGGAGC TCCTGGCCTC 
CCTGGACAAC CAGGAGACAG AGGTGTGCCA GGAGTTGGAC TGGCTGGACC ACCTGGCCCT 
GTTGGACCCC CAGGAGACAA AGGATCATTG GGTCCTCGTG GTGTACCTGG TATCCCTGGA 
CCACAGGGGC CTCCTGGCCA GGATGGTCTA CCTGGAAATC CAGGGGAAAG AGGACCTCCT 
GGAAAACCAG ATGTGTGCAC TGAATGCCCA CCTGGCCCAC CAGGACTGCC AGGCATCCCA 
GGTTTCAAAG GTGACAAAGG TCTCCGAGGA CCACCAGGTA GAGACGGCCA GGATGGGAAA 
ACGGGAAATG CTGGTCCCAG AGGTCCTGCA GGCCCACCTG GGCTGGATGG CCAAAAGGGT 
GCTAAAGGAG ACCGTGGTAT AACTGGGGAT GCAGGAGAAA AAGGTGAACA GGGACACCCT 
GGAATGCCTG GCTTTGCAGG GGCTCCTGGA AGCCCTGGCC CACCTGGACC GGATGGGGCA 
CCGGGACCAA TAGGACCAGC AGGAAACCCA GGAGACATGG GTAAAGATGG CCCTCCAGGT 
CCACAGGGCC CACCAGGTAT TCCTGGACTT CCTGGCATTG CTGGTAATGA TGGCAAAGAT 
GGCAAGCCAG GATCTCTTGG AGAACCGGGA AAACCTGGAG AACCAGGCCT CCCAGGCCAG 
GAAGGTGCCA GAGGCTTACC GGGTTTCAAA GGACAGAGGG GAGATACAGG TCCCCCAGGC 
CCACGGGGGG AACCAGGTGC GACAGGTCCT GCTGGGCGTG GGGGGCCACC AGGGAAGGAT 
GGTGACACTG GCCCCATAGG ACCACAGGGA CCTCGAGGAC TAAGAGGGCA GCCGGGCAAG 
AATGGATTGC CTGGATCTGC AGGAGAACCT GGCCCAGCAG GTAACCCTGG TCCTAAAGGA 
AATAAAGGAG AAAATGGCAG CCCAGGACTC CCTGGCTTCA TAGGACCCCG AGGACCACCT 
GGTGATCCAG GAGAAAAAGG CCCTCCAGGA AAGGAGGGTG CACCAGGGAA ACCAGGTGAA 
ACTGGATCCA AAGGAGAGAG GGGAGAACCT GGCATCAAAG GTGAAAAAGG CCCTCAAGGA 
CAAAAGGGCC CTCCAGGTGA GCCTGGGATA CCTGGTCATA AAGGCCATCC AGGACTGATG 
GGACCCCATG GACCACCAGG AGACACTGGG CAAGTTGGGC CTCCTGGTCC CCCAGGACAG 
CCAGGATTTC CAGGACCACG GGGGGAACCT CCGTCTCTAG AGACTCTGAG AAGACTCATC 
CAAGAGGAGT TAGCTAAGCA GCTAGATGCA AAGTTAGCCT ATCTCTTGGC TCAGATACCG 
CCTGCACATG TGAAAGCATC CCATGGCAGA CCAGGACCTC CTGGTCCCCC TGGGAAAGAA 
GGACTACCAG GAAGAACTGG CCCTCCAGGA GAGCCTGGCC GCCCTGGACA GACTGGATCT 
GAAGGACCAC CCGGACCCAT TGGTCCCAAG GGAGAAAGAG GAGCGAAGGG TGAGAAAGGA 
GATGCTGGCA TTGGCCAGCG AGGTGAAATA GGTCCTCCAG GAATTCCAGG CCTCCCAGGG 
GAGCCTGGCT ATGCCAAAGA TGGGATACCA GGACCACCTG GCCCTCAAGG TGAAGCTGGG 
GTACCTGGTC TCATGGGCCC ACAGGGACCT CCGGGAGCCA ATGGACAGTG TGACCTCGCT 
CAGTGCGCTT ACTATGCAAG CCTGGCTGCC AGGCCCGCCA ATGTCAAAGG GCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL22A1 ( XM_015283174.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015138660.1 Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.

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CloneID	OGa32913
Clone ID Related Accession (Same CDS sequence)	XM_015283174.1
Accession Version	XM_015283174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	collagen alpha-1(XXII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGTGA AGAAGGAGCC CCCGGATTTC CTGGACTGAA AGGTGATAAA 
GGAGAAAAAG GGTCCCTTGG CTTGCCTGGC ACCCCAGGGA AGGATGGAGC AAAAGGAGAA 
GCCGGTGAAC CAGGACAACC AGGAGCATTT GGACTGCCTG GGCCAGCAGG TCCAAAGGGA 
TCTGAAGGAA AACAGGGTCC TCCAGGTATT AAAGGCTACG TAGGAGCACC GGGTCTACAA 
GGAGAAAGAG GAGAAAAGGG AACACGAGGA GACAAGGGAG AACGAGGTTT AGATGGGTTC 
CCAGGAAAGC CAGGTGTGGA GGGGAAACAG GGCCCTGCTG GCAGCCCAGG CCCTCCAGGT 
GGCAGTGGGC CCAAGGGAGA AAAGGGTGAA CCAGGGCTCC CTGGATTGCC AGGCTCTTCA 
GTACCTACTG AAGGCCTGAA AGGAGAGCAA GGAGAGCCTG GTCCACGAGG ACCTCAAGGC 
CAACCTGGGC TTCCTGGACT ACCAGGAGAG ACTGGTCCAG AGGGCAAGCC TGGGGTCCCA 
GGTTTACCAG GTCAAAGGGG TAAGAAGGGA GAACCTGGAT TCCCAGGAAT TAATGGTCTG 
ATTGGCCCTC AGGGACCTCC AGGGCCCCCA GGCATTGCTG GACCGCCTGG ACCACCTGGA 
ACACCAGGAC TGCCAGGAGA AATTGGTGTT GCTGGTAAAA GTGGACCGCC AGGACCAGCT 
GGCTCACCTG GCAAAGATGG ATTAAATGGT CTTCCCGGGC TCCCAGGTCA AAAGGGAGAA 
CAAGGAGAAA AGGGTGAGGA AGGCTCTCCT GGAAAAGCTG GCCCTAAGGG TGAACCAGGA 
AGCCGTGGCC AACCTGGTGA ACAGGGTGAA GTGGGTCTTC CTGGTCTTCA GGGTTTACCT 
GGACTAAGAG GAGAAAAAGG AGACCAGGGA GGAAAGGGAA AAGATGGTCT CCCAGGACTG 
AAAGGGGAAA GAGGAGAAAA GGGTGACACA GGTTCCCCTG GCCCACCAGG CATACCTGGA 
ACAACAGCCT TCTTCACCCC ACACCCGAGG ATTCCTGGTG AACCAGGACC AAAGGGAGAG 
AAAGGAGATC GAGGAACAAG TGGAGAACCT GGATTACCAG GGATCCCAGG TGAACAGGGT 
GTCCCAGGGC CTGTAGGACT ACAGGGGACA AAGGGGCAGA AAGGAGCTCA AGGAGAAGCT 
GGAGCCCCTG GAGAGGCTGG TGCAGTTGGA CCAGTGGGGC CACCAGGACC AAGAGGATTG 
CCAGGCAATG TGGGAGTGCC TGGAAACCCT GGTCTACCAG GGAAGGAGGG AGAAAGAGGG 
GAAAAGGGTG ACCCAGGAAA AGAAGGAAAA GTGGGTTTAC CTGGGCCAGC TGGTGTGCCA 
GGGCCTCCTG GACCACCGGG TTTGCCTGGC AAAGGTAAAG ATGGAGAACA GGGGGAAAGA 
GGCCCACCAG GCTTGCCAGG ACCTTTTGGA CATAAGGGTG AGAGGGGAGC TCCTGGCCTC 
CCTGGACAAC CAGGAGACAG AGGTGTGCCA GGAGTTGGAC TGGCTGGACC ACCTGGCCCT 
GTTGGACCCC CAGGAGACAA AGGATCATTG GGTCCTCGTG GTGTACCTGG TATCCCTGGA 
CCACAGGGGC CTCCTGGCCA GGATGGTCTA CCTGGAAATC CAGGGGAAAG AGGACCTCCT 
GGAAAACCAG GTACCTCACC CCTGCTATCT CCAGAGGACA TAAATCTCTT AGTGAAGGAT 
GTGTGCACTG AATGCCCACC TGGCCCACCA GGACTGCCAG GCATCCCAGG TTTCAAAGGT 
GACAAAGGTC TCCGAGGACC ACCAGGTAGA GACGGCCAGG ATGGGAAAAC GGGAAATGCT 
GGTCCCAGAG GTCCTGCAGG CCCACCTGGG CTGGATGGCC AAAAGGGTGC TAAAGGAGAC 
CGTGGTATAA CTGGGGATGC AGGAGAAAAA GGTGAACAGG GACACCCTGG AATGCCTGGC 
TTTGCAGGGG CTCCTGGAAG CCCTGGCCCA CCTGGACCGG ATGGGGCACC GGGACCAATA 
GGACCAGCAG GAAACCCAGG AGACATGGGT AAAGATGGCC CTCCAGGTCC ACAGGGCCCA 
CCAGGTATTC CTGGACTTCC TGGCATTGCT GGTAATGATG GCAAAGATGG CAAGCCAGGA 
TCTCTTGGAG AACCGGGAAA ACCTGGAGAA CCAGGCCTCC CAGGCCAGGA AGGTGCCAGA 
GGCTTACCGG GTTTCAAAGG ACAGAGGGGA GATACAGGTC CCCCAGGCCC ACGGGGGGAA 
CCAGGTGCGA CAGGTCCTGC TGGGCGTGGG GGGCCACCAG GGAAGGATGG TGACACTGGC 
CCCATAGGAC CACAGGGACC TCGAGGACTA AGAGGGCAGC CGGGCAAGAA TGGATTGCCT 
GGATCTGCAG GAGAACCTGG CCCAGCAGGT AACCCTGGTC CTAAAGGAAA TAAAGGAGAA 
AATGGCAGCC CAGGACTCCC TGGCTTCATA GGACCCCGAG GACCACCTGG TGATCCAGGA 
GAAAAAGGCC CTCCAGGAAA GGAGGGTGCA CCAGGGAAAC CAGGTGAAAC TGGATCCAAA 
GGAGAGAGGG GAGAACCTGG CATCAAAGGT GAAAAAGGCC CTCAAGGACA AAAGGGCCCT 
CCAGGTGAGC CTGGGATACC TGGTCATAAA GGCCATCCAG GACTGATGGG ACCCCATGGA 
CCACCAGGAG ACACTGGGCA AGTTGGGCCT CCTGGTCCCC CAGGACAGCC AGGATTTCCA 
GGACCACGGG GGGAACCTCC GTCTCTAGAG ACTCTGAGAA GACTCATCCA AGAGGAGTTA 
GCTAAGCAGC TAGATGCAAA GTTAGCCTAT CTCTTGGCTC AGATACCGCC TGCACATGTG 
AAAGCATCCC ATGGCAGACC AGGACCTCCT GGTCCCCCTG GGAAAGAAGG ACTACCAGGA 
AGAACTGGCC CTCCAGGAGA GCCTGGCCGC CCTGGACAGA CTGGATCTGA AGGACCACCC 
GGACCCATTG GTCCCAAGGG AGAAAGAGGA GCGAAGGGTG AGAAAGGAGA TGCTGGCATT 
GGCCAGCGAG GTGAAATAGG TCCTCCAGGA ATTCCAGGCC TCCCAGGGGA GCCTGGCTAT 
GCCAAAGATG GGATACCAGG ACCACCTGGC CCTCAAGGTG AAGCTGGGGT ACCTGGTCTC 
ATGGGCCCAC AGGGACCTCC GGGAGCCAAT GGACAGTGTG ACCTCGCTCA GTGCGCTTAC 
TATGCAAGCC TGGCTGCCAG GCCCGCCAAT GTCAAAGGGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015138660.1
CDS	358..5202
Translation	MSVMGRCHLPCLLILFTLMFSEERTEAQRAGCKNVHYDLVFILDASSSVGKEDFEKVRQWVSNLVETFEIGPDKTRVGVVRYSDRPTTEFDLGKYKTCEEIKEAARKIRYYGGNTNTGDALRYINTYSFSKEAGGRLSDRTVKKVAILLTDGRSQDYVLDPANAARQAGIRIFAVGVGEALKEELDEIASEPKSAHVFHVSDYNAIDKIRGKLRRRLCENVLCPNVHVEGDRFKHTNGETDEIPGFDLMDSFSVKQIFGMKENGIQSSYVRLGSFPVVQKTEDVFPQGLPDEYAFVTTFKFRKASRKEDWYIWQIIDKYGIPQVSIRLDGENKALEYNAIGLTKDAVRVVFRSARVSDLFDRSWHKIALSIQSAAVSLYIDCKLVQMLPIEDRENIDIQGKTVIGKRLYDSVPIDFDLQRMVIYCDSRHAELETCCDIPSGPCQVTVLTEAPPVEVRPTVGSEQVAHLKTVNCSCPAGQKGERGSIGPEGPKGQKGESGSQGLPGPRGEKGESGRAYSRGEKGEKGEEGAPGFPGLKGDKGEKGSLGLPGTPGKDGAKGEAGEPGQPGAFGLPGPAGPKGSEGKQGPPGIKGYVGAPGLQGERGEKGTRGDKGERGLDGFPGKPGVEGKQGPAGSPGPPGGSGPKGEKGEPGLPGLPGSSVPTEGLKGEQGEPGPRGPQGQPGLPGLPGETGPEGKPGVPGLPGQRGKKGEPGFPGINGLIGPQGPPGPPGIAGPPGPPGTPGLPGEIGVAGKSGPPGPAGSPGKDGLNGLPGLPGQKGEQGEKGEEGSPGKAGPKGEPGSRGQPGEQGEVGLPGLQGLPGLRGEKGDQGGKGKDGLPGLKGERGEKGDTGSPGPPGIPGTTAFFTPHPRIPGEPGPKGEKGDRGTSGEPGLPGIPGEQGVPGPVGLQGTKGQKGAQGEAGAPGEAGAVGPVGPPGPRGLPGNVGVPGNPGLPGKEGERGEKGDPGKEGKVGLPGPAGVPGPPGPPGLPGKGKDGEQGERGPPGLPGPFGHKGERGAPGLPGQPGDRGVPGVGLAGPPGPVGPPGDKGSLGPRGVPGIPGPQGPPGQDGLPGNPGERGPPGKPGTSPLLSPEDINLLVKDVCTECPPGPPGLPGIPGFKGDKGLRGPPGRDGQDGKTGNAGPRGPAGPPGLDGQKGAKGDRGITGDAGEKGEQGHPGMPGFAGAPGSPGPPGPDGAPGPIGPAGNPGDMGKDGPPGPQGPPGIPGLPGIAGNDGKDGKPGSLGEPGKPGEPGLPGQEGARGLPGFKGQRGDTGPPGPRGEPGATGPAGRGGPPGKDGDTGPIGPQGPRGLRGQPGKNGLPGSAGEPGPAGNPGPKGNKGENGSPGLPGFIGPRGPPGDPGEKGPPGKEGAPGKPGETGSKGERGEPGIKGEKGPQGQKGPPGEPGIPGHKGHPGLMGPHGPPGDTGQVGPPGPPGQPGFPGPRGEPPSLETLRRLIQEELAKQLDAKLAYLLAQIPPAHVKASHGRPGPPGPPGKEGLPGRTGPPGEPGRPGQTGSEGPPGPIGPKGERGAKGEKGDAGIGQRGEIGPPGIPGLPGEPGYAKDGIPGPPGPQGEAGVPGLMGPQGPPGANGQCDLAQCAYYASLAARPANVKGP

Target ORF information:

RefSeq Version	XM_015283174.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus collagen type XXII alpha 1 chain (COL22A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283174.1

ORF Insert Sequence:

ATGTCTGTTA TGGGAAGATG CCATTTACCC TGTCTCCTAA TTCTTTTCAC ACTTATGTTC 
AGTGAGGAGA GAACTGAGGC ACAGAGAGCA GGTTGCAAGA ATGTTCATTA TGACCTTGTT 
TTCATCTTGG ATGCCTCCTC TAGTGTTGGA AAAGAAGATT TTGAGAAGGT TCGGCAGTGG 
GTGTCTAATT TGGTGGAAAC TTTTGAGATT GGTCCTGACA AAACCCGCGT CGGTGTGGTG 
CGATACAGTG ACCGGCCAAC CACTGAGTTT GACCTGGGAA AGTACAAAAC CTGTGAGGAA 
ATCAAAGAGG CCGCCCGAAA AATTAGGTAT TATGGGGGTA ATACAAACAC TGGAGATGCT 
CTCCGGTACA TCAACACTTA CAGTTTTTCC AAAGAGGCAG GTGGGAGACT TAGTGATCGA 
ACTGTTAAAA AAGTGGCTAT CCTTCTGACT GATGGAAGGA GCCAGGATTA TGTCTTGGAT 
CCGGCCAATG CAGCTCGTCA GGCTGGAATT AGGATCTTTG CTGTGGGTGT CGGCGAAGCC 
TTAAAAGAAG AATTGGATGA GATTGCTTCA GAACCAAAGT CTGCTCACGT GTTTCATGTC 
TCCGATTACA ATGCCATCGA TAAAATTCGG GGGAAGCTGA GAAGACGTCT CTGTGAAAAT 
GTTCTGTGTC CAAATGTTCA TGTGGAAGGA GATCGATTCA AGCACACTAA TGGGGAGACA 
GATGAGATTC CAGGGTTTGA CCTGATGGAT TCGTTCAGTG TGAAACAGAT CTTTGGAATG 
AAAGAAAATG GAATTCAGAG CTCCTATGTA CGACTTGGAA GTTTCCCTGT TGTTCAGAAA 
ACTGAGGATG TATTCCCACA AGGTCTGCCT GATGAATATG CCTTTGTAAC TACCTTCAAA 
TTCCGGAAGG CTTCCAGAAA AGAAGATTGG TATATTTGGC AGATTATTGA CAAGTATGGC 
ATACCACAGG TCTCGATCCG ACTGGATGGA GAAAACAAAG CTTTGGAGTA CAATGCCATT 
GGGCTCACCA AGGATGCTGT GAGAGTGGTC TTTCGAAGTG CACGAGTCAG TGATCTTTTT 
GATCGCAGCT GGCACAAAAT TGCCCTCAGC ATTCAGTCAG CAGCTGTCTC ACTGTACATA 
GACTGCAAGC TTGTGCAGAT GCTGCCTATT GAAGACAGGG AAAATATCGA CATTCAAGGA 
AAGACTGTGA TTGGCAAACG CTTGTATGAT AGTGTCCCCA TTGATTTTGA TCTTCAGCGG 
ATGGTTATTT ACTGTGATTC TCGGCATGCT GAGCTGGAGA CCTGCTGTGA CATTCCTTCA 
GGACCATGCC AGGTCACGGT CCTAACAGAA GCACCTCCTG TGGAAGTGAG GCCTACTGTT 
GGCAGCGAGC AAGTTGCCCA CCTAAAGACT GTCAATTGCT CCTGTCCTGC TGGACAAAAG 
GGGGAGAGAG GCTCAATTGG CCCTGAAGGT CCTAAAGGTC AAAAGGGAGA AAGTGGCAGC 
CAAGGGCTTC CTGGACCCAG AGGAGAAAAA GGAGAGTCAG GCAGAGCCTA TAGCAGAGGA 
GAAAAAGGAG AAAAGGGTGA AGAAGGAGCC CCCGGATTTC CTGGACTGAA AGGTGATAAA 
GGAGAAAAAG GGTCCCTTGG CTTGCCTGGC ACCCCAGGGA AGGATGGAGC AAAAGGAGAA 
GCCGGTGAAC CAGGACAACC AGGAGCATTT GGACTGCCTG GGCCAGCAGG TCCAAAGGGA 
TCTGAAGGAA AACAGGGTCC TCCAGGTATT AAAGGCTACG TAGGAGCACC GGGTCTACAA 
GGAGAAAGAG GAGAAAAGGG AACACGAGGA GACAAGGGAG AACGAGGTTT AGATGGGTTC 
CCAGGAAAGC CAGGTGTGGA GGGGAAACAG GGCCCTGCTG GCAGCCCAGG CCCTCCAGGT 
GGCAGTGGGC CCAAGGGAGA AAAGGGTGAA CCAGGGCTCC CTGGATTGCC AGGCTCTTCA 
GTACCTACTG AAGGCCTGAA AGGAGAGCAA GGAGAGCCTG GTCCACGAGG ACCTCAAGGC 
CAACCTGGGC TTCCTGGACT ACCAGGAGAG ACTGGTCCAG AGGGCAAGCC TGGGGTCCCA 
GGTTTACCAG GTCAAAGGGG TAAGAAGGGA GAACCTGGAT TCCCAGGAAT TAATGGTCTG 
ATTGGCCCTC AGGGACCTCC AGGGCCCCCA GGCATTGCTG GACCGCCTGG ACCACCTGGA 
ACACCAGGAC TGCCAGGAGA AATTGGTGTT GCTGGTAAAA GTGGACCGCC AGGACCAGCT 
GGCTCACCTG GCAAAGATGG ATTAAATGGT CTTCCCGGGC TCCCAGGTCA AAAGGGAGAA 
CAAGGAGAAA AGGGTGAGGA AGGCTCTCCT GGAAAAGCTG GCCCTAAGGG TGAACCAGGA 
AGCCGTGGCC AACCTGGTGA ACAGGGTGAA GTGGGTCTTC CTGGTCTTCA GGGTTTACCT 
GGACTAAGAG GAGAAAAAGG AGACCAGGGA GGAAAGGGAA AAGATGGTCT CCCAGGACTG 
AAAGGGGAAA GAGGAGAAAA GGGTGACACA GGTTCCCCTG GCCCACCAGG CATACCTGGA 
ACAACAGCCT TCTTCACCCC ACACCCGAGG ATTCCTGGTG AACCAGGACC AAAGGGAGAG 
AAAGGAGATC GAGGAACAAG TGGAGAACCT GGATTACCAG GGATCCCAGG TGAACAGGGT 
GTCCCAGGGC CTGTAGGACT ACAGGGGACA AAGGGGCAGA AAGGAGCTCA AGGAGAAGCT 
GGAGCCCCTG GAGAGGCTGG TGCAGTTGGA CCAGTGGGGC CACCAGGACC AAGAGGATTG 
CCAGGCAATG TGGGAGTGCC TGGAAACCCT GGTCTACCAG GGAAGGAGGG AGAAAGAGGG 
GAAAAGGGTG ACCCAGGAAA AGAAGGAAAA GTGGGTTTAC CTGGGCCAGC TGGTGTGCCA 
GGGCCTCCTG GACCACCGGG TTTGCCTGGC AAAGGTAAAG ATGGAGAACA GGGGGAAAGA 
GGCCCACCAG GCTTGCCAGG ACCTTTTGGA CATAAGGGTG AGAGGGGAGC TCCTGGCCTC 
CCTGGACAAC CAGGAGACAG AGGTGTGCCA GGAGTTGGAC TGGCTGGACC ACCTGGCCCT 
GTTGGACCCC CAGGAGACAA AGGATCATTG GGTCCTCGTG GTGTACCTGG TATCCCTGGA 
CCACAGGGGC CTCCTGGCCA GGATGGTCTA CCTGGAAATC CAGGGGAAAG AGGACCTCCT 
GGAAAACCAG GTACCTCACC CCTGCTATCT CCAGAGGACA TAAATCTCTT AGTGAAGGAT 
GTGTGCACTG AATGCCCACC TGGCCCACCA GGACTGCCAG GCATCCCAGG TTTCAAAGGT 
GACAAAGGTC TCCGAGGACC ACCAGGTAGA GACGGCCAGG ATGGGAAAAC GGGAAATGCT 
GGTCCCAGAG GTCCTGCAGG CCCACCTGGG CTGGATGGCC AAAAGGGTGC TAAAGGAGAC 
CGTGGTATAA CTGGGGATGC AGGAGAAAAA GGTGAACAGG GACACCCTGG AATGCCTGGC 
TTTGCAGGGG CTCCTGGAAG CCCTGGCCCA CCTGGACCGG ATGGGGCACC GGGACCAATA 
GGACCAGCAG GAAACCCAGG AGACATGGGT AAAGATGGCC CTCCAGGTCC ACAGGGCCCA 
CCAGGTATTC CTGGACTTCC TGGCATTGCT GGTAATGATG GCAAAGATGG CAAGCCAGGA 
TCTCTTGGAG AACCGGGAAA ACCTGGAGAA CCAGGCCTCC CAGGCCAGGA AGGTGCCAGA 
GGCTTACCGG GTTTCAAAGG ACAGAGGGGA GATACAGGTC CCCCAGGCCC ACGGGGGGAA 
CCAGGTGCGA CAGGTCCTGC TGGGCGTGGG GGGCCACCAG GGAAGGATGG TGACACTGGC 
CCCATAGGAC CACAGGGACC TCGAGGACTA AGAGGGCAGC CGGGCAAGAA TGGATTGCCT 
GGATCTGCAG GAGAACCTGG CCCAGCAGGT AACCCTGGTC CTAAAGGAAA TAAAGGAGAA 
AATGGCAGCC CAGGACTCCC TGGCTTCATA GGACCCCGAG GACCACCTGG TGATCCAGGA 
GAAAAAGGCC CTCCAGGAAA GGAGGGTGCA CCAGGGAAAC CAGGTGAAAC TGGATCCAAA 
GGAGAGAGGG GAGAACCTGG CATCAAAGGT GAAAAAGGCC CTCAAGGACA AAAGGGCCCT 
CCAGGTGAGC CTGGGATACC TGGTCATAAA GGCCATCCAG GACTGATGGG ACCCCATGGA 
CCACCAGGAG ACACTGGGCA AGTTGGGCCT CCTGGTCCCC CAGGACAGCC AGGATTTCCA 
GGACCACGGG GGGAACCTCC GTCTCTAGAG ACTCTGAGAA GACTCATCCA AGAGGAGTTA 
GCTAAGCAGC TAGATGCAAA GTTAGCCTAT CTCTTGGCTC AGATACCGCC TGCACATGTG 
AAAGCATCCC ATGGCAGACC AGGACCTCCT GGTCCCCCTG GGAAAGAAGG ACTACCAGGA 
AGAACTGGCC CTCCAGGAGA GCCTGGCCGC CCTGGACAGA CTGGATCTGA AGGACCACCC 
GGACCCATTG GTCCCAAGGG AGAAAGAGGA GCGAAGGGTG AGAAAGGAGA TGCTGGCATT 
GGCCAGCGAG GTGAAATAGG TCCTCCAGGA ATTCCAGGCC TCCCAGGGGA GCCTGGCTAT 
GCCAAAGATG GGATACCAGG ACCACCTGGC CCTCAAGGTG AAGCTGGGGT ACCTGGTCTC 
ATGGGCCCAC AGGGACCTCC GGGAGCCAAT GGACAGTGTG ACCTCGCTCA GTGCGCTTAC 
TATGCAAGCC TGGCTGCCAG GCCCGCCAAT GTCAAAGGGC CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL22A1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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