CEP250 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CEP250
Entrez Gene ID	419138
Full Name	centrosomal protein 250
General protein information	Preferred Names centrosomal protein 250 Names centrosome-associated protein CEP250 centrosomal protein 250kDa
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015296253.1	XP_015151739.1	centrosome-associated protein CEP250 isoform X1
XM_004946945.3	XP_004947002.1	centrosome-associated protein CEP250 isoform X2
XM_004946947.2	XP_004947004.1	centrosome-associated protein CEP250 isoform X3
XM_004946950.2	XP_004947007.1	centrosome-associated protein CEP250 isoform X5
XM_025142331.1	XP_024998099.1	centrosome-associated protein CEP250 isoform X4
XM_004946947.3	XP_004947004.1	centrosome-associated protein CEP250 isoform X3
XM_015296253.2	XP_015151739.1	centrosome-associated protein CEP250 isoform X1
XM_004946945.2	XP_004947002.1	centrosome-associated protein CEP250 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100858952	XP_003642497.2
Homo sapiens (human)	CEP250	NP_009117.2
Pan troglodytes (chimpanzee)	CEP250	XP_003316929.1
Mus musculus (house mouse)	Cep250	NP_001123471.1
Bos taurus (cattle)	CEP250	XP_005215062.1
Gallus gallus (chicken)	CEP250	XP_417323.4
Macaca mulatta (Rhesus monkey)	CEP250	XP_001099508.1
Canis lupus familiaris (dog)	CEP250	XP_542975.3
Rattus norvegicus (Norway rat)	Cep250	XP_006235436.1

Gene Ontology

The following CEP250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CEP250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa146922	XM_025142331.1 Latest version!	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa17583	XM_004946945.3 Latest version!	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa17584	XM_004946947.3 Latest version!	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa17587	XM_004946950.2 Latest version!	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa44661	XM_015296253.2 Latest version!	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OGa17584	XM_004946947.2	Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa44661	XM_015296253.1	Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OGa17583	XM_004946945.2	Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CEP250 ( XM_025142331.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024998099.1 Gallus gallus centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

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CloneID	OGa146922
Clone ID Related Accession (Same CDS sequence)	XM_025142331.1
Accession Version	XM_025142331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCC GAGAGGAGCT GGAATTGAAG GAGAGTGAAT GGCGCAGTGA GCGTGAGTTT 
TATGACAGCT ACTTAAGGGG TGAACATAAC CGTCTTCTGA GCCTATGGCG TCAGGTGCTG 
ACTTTCCGCC GTCACTTCCT GGAAATGAAG ACTGCCACTG ACAGAGATTT GTCAGAGCTG 
AAGGCAGAGC AAATGAGACT CTCTGGATCT ATACTTGTAA GCTGCTTCCG TTTGAACTCT 
GGCGTACAGC TCTGGGAGTC TGTCACTTTG GGGAGGCCTG TCCTAAAGGA TCAAGCACAG 
CAGCAAGTGG AAAGGGAAAT AAACCAGGAG GCTTTGGAAG TGATGTGCTT ACAAGTTGAT 
GAGGACCTGG AGAAGAAAGA GCTTCAAGAT AGGGTGATGG AGCTCTCAGC CTTGCTTGTA 
CAGTCTCAGA AACAAAATGA AGAGAAAGAG AAGACCGTGA AAACACTTAA CGACACTGTG 
GAGATGCTAG AAGCAAGTCG GTTAGAGGTA GAATACGAAG CTTCATTAAC TAAAAATGCC 
AAAGAAGAGA ACCTTGCCCT TCAGAAGCTA ATCAAAGAGA TAACTGAGGT GGTGTTAGAT 
GACAGTGACA GCACAGTCGG CATTGTCCAG CGTCCAGAGT CTAGCAACGT CCTCTCTTGT 
CTGAGCTCCC TTGATGCAGA GCATGCCTTT GTGTTGGTTC AGGAAACACT GGCAAGGAGG 
CAAAAAGCAA TACAGGCCCT AAAAGAAGAG CTTTCTACCA GGCAAGACTC TATCAGTTTT 
CTGCAGCACC AGCACAGACA GCAGGAAGAG AAGTGCAAGA AGCTGCAGCA AAGGCTTGAG 
CAACTGGAGG AGGAAAACAA GATGTCTAAT AGCCACCAGC AGCACCTCAA GTCTCTGGTA 
CTAGCGCTCA AGTGTGACTG TGAAAACCTA GAGAAAATCA GAGGAGAACT ACAGCAAAAG 
CTGGAATTAA GTGAGCAAGA AGCCTCACGT CTGCGTCAGA GTAACACTGA GCTGCAGCTG 
AAGGAAGATT CAGCCCAAGG GGAAAAGGTG GAGCAGCAGC TGGCAATGGA GAGAGCACAT 
CATGACCATG AGCTCGTTCT GAAGGACTTA GCTGCACTTG AAGAAAAACA TTCGTTGTTA 
CAGAATGAAT TGGTAGCCGC GAGGGAGAAG CTGGAGGAAT CACACCTTCA GAGGGATCTG 
CTGAAGCAAG AGAAACATGA GCTTACAGTG GCACTGGAAA AGGCAGAGAA ATCAGTAGCA 
GCATTGACAG GGGCTCAGAA TAAGTTGAAT TCTGAAATAG CTGACCTACA TACTGCTGCA 
GCAAAGATGA GCAGTATTAA TGAAGCTCTT GCACTGGATA AAGTGGAACT GAACAAGCTT 
GTGTTGCAGC TGGAACAAGA GAATGAACTT CTGTCAGATA AAGTGAATGA GATGGAGAGA 
GCAAAGGTCT CTGAACAGGA GAAGATAACC TTGTATAAAA GAACAAATGA AGAGCTTTGT 
GCAGAGAAAT CCCACCTGGA GAAGCTGCTG AAGAAAGCAG AGGAGCAACA GGAAGAGCTG 
CAGATGCAAC TGAGAATACT GGCAGAGGAA AAGGAAGAAA CCCTGGAGAA ACTCAACCAG 
GTTTACCGCC AGCAAGAGTC AGCTAGCAGT GGTTTGGAGC AGTTGCGCCA ACAGTCCTCT 
CGCCAAGAGG ATGCAGTGGC CAAGGTGTCC AAAGAGAAGG AATTCCTGGT GCATGAGAAG 
GCCGCCCTTG AGGTGCGACT GGCAGCTGTG GAACGGGACA GACAAGGCCT TTCAGAGCAG 
CTGGCAGAGG CCAGGTCAGT GAAGGACACC CTGGAATCCA GCCTGTTTGA GGCTCAGCAG 
CAGTTATCTC ACCTGGAGAT CACCAAGAGT CAGCTTGAAA TCCAGCTTCA TACAGTCGTT 
CAGGCTAAGG AAGTGATACA AGGGGAAGTG AAGTGTCTTC AATATGAGCT GGAAACAGAG 
AGATGTCTAA TGAAGCAGGA ACAGAAAAAC ATGGCACAAC GGCTCATTCA GATAGAAGAG 
CAGCATAACA ATACCCTGAA ACTTCAGCAA ACTGATCATG AAGTGGAAAT AAACAAACTC 
CTACAAGACC TGGCAAGTGA ACGGGAGGGG CGCCAGTTAG AGCTACAGGA GATGCTGGAG 
CTCTGGGAAA AGGAGAAAGC AGAGGCAGAA GGGCAGCATG AGAAGAAGCT GTTTCATATG 
AAGGAGAAAG TTGCTACCAT GCAAGCTCAA CAAGAAGAGG AACGAACCAG AGTTGAAAGT 
GCCAATCAAG AGATTTTGAC AGAAAAGGAG AACGAGAAGA AAGCTTTATT AGAGACACTA 
CTCCAAACTC AGGGAGAACT AACAGAAGCC TGCCATCAGC TTGAGCAACT TAGGCAGGAG 
GTGAAAGAGC AGCAGGAGTA TGAGCAGAAC ATCACAGAAA AACTGCAAGC AGAGCTACAG 
GAAACTCACT GTAAAATCAA GATGGTGGAG AATATGCACA AGGAAGAAAT GGAAAACATC 
AAAGAAGAAA AGAATATCCT TCTCCAGCAG AGGGATGATC TACAAAAACA GGTAGAAGAG 
CTGACATCTC AGCTAGCAGC CTCCGAAGAG TCCCATCAAG CAATTGGCCA TAAAGCTCAG 
CAAGAGCTGA GTGAGGCACA AGAGTTGTCC AGGCAGAAGG TATTGGAGGT TGTGCACCTT 
CAGAAAATCT TGGAGGAGGA GAGGGGCCAA TGGGAAGAGG TGGAACATCA GAACAAAGAG 
CTGCAGGTGT GCCTGCAGTC CTTAGAGGGG GAAAGGAGTC GATGGGAAGA AGTGGAACGT 
CACAACACAG AATTTCAGGC TTCATTGAAG GCCCTAGAGA GCGAAAGAGA AAGGCTATCT 
CTGTCTCTGG AACAGAAGGA ATTGAGCCTC AAGACCTTGG AAGAAAACAA CCTTGTCCAG 
CAGAACGAGG TGTCTAAACT ACATTCTGCT ATTCAACAGG CTCAGCAGCT CCACTCAGAC 
CACAGAAGAG AAATACAAGA GCTCAACAAC CAGGTACAGA CACTACAGGA AGTGGTGCTG 
GAGAAGGAAG CTAGCCTGGC AGCTCGGGAG AAGCAGCTAT TACAGGATCT GGAGGAGTCT 
CGAGCAGGCG AACGGTGCTT GAGAGACTCT CTGCACGTGC TAGAGGCTGA GATGGCTGAA 
CTTCATCTGA GGCTTTGTAG CACAGAGAAC AGAGCAAAGG CATTGGCTAC AGAGTGCCAG 
CAGGCTAATA ATGCCCACTG TGAAGCCCAG TCTCAGCTGG ACAAATTGCA CTTGGTTCTT 
CACCACGTGC TGTGTGACAG CACTGATAAA AGCAGAGACT TAGTCGCTTG GAGTTCAGAA 
CAGGATCATG TCTGGGGCCT AACTGTTTCT CAGGCCAAGG ATCTCCATGT AGAGCTCACA 
GTGGACAGAG TGGCAGCAGC CCTGCAAGAC CTGCGTCAGG ACCTGAAGCA AACTCAGCAA 
GATCTGAATG ATGCTGGGAA GAAAGTGCAG GACCTGGAAC TGGAGCTGAG CAAGAGGCAA 
GCTGAGAGGG ACCACTTCAG TGCCCACAAT CAGGAGCTAC AGAAACAGTT GGCTCAAAGC 
CAGGAAGAGA CACAGATGGT AGAAGGGAAG AAGAACTCTC TACAAGCAGC CTTGCAGGCA 
GAAGCTGCTG CTCTAAAGAA GGAGGCTATT ACACTACATC AAGAGGTGGC ATCTTTGGAA 
AGGAAACTTG AGAGCGCTGA GAAGCAAAAA AAGGATGTGC TGCATGAACG GGATAGACTG 
CAAGCAGTTG AAGAAAAACT GATGTGGGAG ATAAAAATTC TTCAGGAGTC AGTCACAGCC 
TCAGAAACAC GAGCAAACAC AGCAGCAGAT ATGAATCACT CCCTTGAACA GGAACTACAA 
ACTACATTGT CTGTCTTGAA AACAAAAAAT AAGGAAGTGG ATGCTCAATG GGAGAAAATC 
CAGATGCTAC AGAAAGAGGC AGCAGATGTA AAAGCTTTGC AGGAGAATCT TATTCAAGTG 
AATGCCATCC TGTCAAAGAG AGAGGGAGAA ATGAAGTTAT ACCAGGAACA GATGAGAATG 
CTGGAAAACC AGAAAGAAAT GCATAAAACT ACTCTAGATC AAGTTATTAA GGACATAAAA 
GAGAAAAAAG AGAAGACAGA ATCCCAGCAA GAACAAATAC AGGAGTTAGA GAAGCAGCAA 
GAACTGCAAA GGACTGTTAT AAGCAAAATG AGCAAAGACC TGGAAGACAG AGATAAGGAG 
ATCAGATCCC AGCAAGAAGA AATATGGGAG CTGGAGAAGC AACAAGAACT GCAGAGGACT 
GTTGTAAGCA AGATGACTAA AGACCTGGCA CACAGAGACC AGGAGATCCA ATCCCAACAA 
GAAGAGATTC AGGAGCTAGA GAAGGAGCGA GAATTGCAGA GGACTGCTGC AAGTAAGATG 
AGCAAAGATC TAAAGGAGAG AGATGAGAAG ATAAGATCCC AGCAAGAACT GATAGAGGAG 
CTAGAGAAGC AGCAAGAACT GCAGAGGACT GCCTTAAGCA AAATGAGCAA AAACCTGGAG 
GAGAGAGACC AGGAGATCAA ATCCCAGCAA GAACTGATAG AGGAGCTAAA GAAGCAACAA 
GAACTACAGA GGACTGCTGT GAGCAAAATG AACAAAGACC TGGAGGAGAG AGACCAGGAG 
ATTAGATCCC AGCAAGAAGA GATACAGGAA CTGGAAAAGC AACGAGAACT GCAGAGGACT 
ATTTTAAGCA AGATGAGCAA AGATCTGGAA GAGAAAGATC AGGTGATCAA GTTCCAGGAA 
GGGAAGGTAA TGATTCTAGA ACAACATGGT ACATCACAGG TGAGAAGTTT GCTAGTGGAT 
CTTGATCATA TGAAGGGAAA CTTGAAGGAG AAAAACTTAG AGCTGATGTC TCTAAATCAG 
CAGATCAAAG AACTGGAGAT GGAGAGAGAA GAGGTGAAAT CTTTGCACAC AAGCCTTGAA 
CAGCTTAGAG CAGTTCTTAG GGATAGAGAG AATGAGTCTG ACTCTCAAAG GGATCAGTTA 
AGACTCTTTC AGCAGTACAA GGAGCATCAA GAGGAGTACC TGCAAGAGCT CCAGGATAAA 
GTAGAAAAAA TGACACTTTC CTTATCTAAA AAAGATCAAG AGCTCGAGTC ACAGCAAAAG 
CAAATCCAGG AAGCTGAAGA AGTCATGGAA AAGAAGTTGA AGACTGTTTG TGACCAACTG 
GAGCAGACAT TAGAAACCTT AAAAGAAAAG GAAAGACTCC TAGACATCCA AAAGCAACAA 
ACAAGGGAAT ATGAGGAAAA AACAGAACAG ATGAATGTCT TATGTAGAGA CTTAGAATAC 
ACTAAGGCAA TATTGAGAGA GAAGGATTTA ATGATTGAAT CTCAGAAGGA ACTGATTGAG 
ACCTTCCAAA AACAAGAAGA CGACTCTATG CAGCAGAAAG AAATTCTGCA GCATCTTAAA 
GGGGCTTTAA AGGAACAAGA ACAAGAAACT TTATCTCTTA GAAAGCAGTG TGAGGCATTT 
AAAGAAAAGG AGGAAAAGCA CAAAACTGAT CAAGTGATTC TTCAAGAAAC AAAAATCACT 
CTGAAAGAAA GAGAAAGAAA GATAGAGGTT TTAGAGAAAG CTGTCTCTAA GCTTCAACAA 
CAAAATGATG ATACGATGAT GCAGACTACA GCTATAGTGC AAAAACTACA ATGTGCTGAA 
TCTTCCTTAG CAGCTAGGGA TCAAGAGATA GCATCTCTGA AAGAGCATGT CCAGGAACTT 
CAGAAGCAGA AGGAGTCGGA AGCTAAACAG GTCAAAAGTC TACAGCAGGA TCTAAACAAA 
ATGAGCAAAA CAATGAAGAA GAACAATTTG CAGATTCTCA AACAGTCGGA GCAGATTAAT 
GTGTTTCATA TTTGTGAAGA AAGTAGGAAA GTAGCACTAA CATCTTGCCA GAATCAAGTG 
AGTCTGCTTG AGGAAGTGGT GAGAAAGAGA GATGAAGACA ATGAAACTCT TCTGCAAAAA 
CTCCAGCACC AAGAAGAAGA ACTGAAGACC TTGCAGAATC TCCAGCTTAT GCTATCTGAG 
AAGAAAGAGG AGATTAAACG TCACGGAGAG GAAGAGAAGT TCCCAGAAGA AGCCTTGCAT 
GGGAGTGAAT GGGAGACGAA GGCTCAAGGT GAGCAAAAAA AGTCAGAAGA TGAGGAAATA 
AGAGGTCTTC ATGAAGATCT CCAGTATGTT CAGCAGACTC TGACAAAGAA GGATGAAGAG 
ATCAAATACC AGAGAGACAG AGTCAAGTAT TTAGAGAAAA CTCTGGCAGG GAAAGAAAAA 
GAGCTGAGGA GGCAGAGTGA ACTCTTGAAA CAATTAACAT CAGCTCTGCA TTGGAAAGAT 
GAAGGGGAGA CTCTAAAGAA ACAAATCCAG AAACTCCAAA AATGGGAGGA AGAAGAAGCA 
GAGAACAGGA GGGTTCTCCA AGAGAGAGAC CAATTCTTGC AAAGGCAGAA GGAACTAGCC 
CAACAACTGG AAGATGAGAG GAAAGTAAAG GGGGAAGAAC TGAAGCGTGT GATTGCTATT 
TTGAAGCAGA CTGAAAGTGG CGAAATCGAA TGGAAAGAGA AGGCACAAGC ACTGACTCTT 
GCCCTTTCTA AGAGTGAAAT GACCAATGGG ACTTTGAGGG AAGAAATAGC CATTCTGCAG 
AGTATGGTTT CAGAGAGGGA CAAGGACCGG TTTCATCTTC AGGAGGCTTT TGAAGGCGGT 
GAGAACCTAT CGTGGCTCTT GGAAAAGAAG CTCCTATGGC AGCGGCTGGA GTGTCTGCAG 
CAAGCAGTTG CAAGGCTGGA ACATGAAAAG TCTGAGCTGA AGCAACTCAA TGTTGAGCTC 
AAGATAACTC TTGAGCAGGT GGAACGTGAA CGGAGGAGGC TGAAGAGAAG TTGTAGTCAT 
CAGTCACAGC CAAATGCAAA CAGGTTTTCT CTCTCGGAAT CTGATGAGGA CAAGATGCCT 
GCTTCTAGAA AGGAGGAGGC TCGTGCTCTC TGCAGTCAAT TAGCTGAGTT ACAGAACCAG 
GTGACTCTTC TGCAGCAGCA GTTGGCACAA GAACGAAAAT ACAAGCAGGA CTATATTGAG 
TGTTGTGCAA AAACCAGTCA GGAGCTTTTG GACCTTCACC AAGAGCTGTC TTACTCCCTG 
GAGGCTGTGA TCAGGGAGCC CAAAGCTGCT GTTCTGGAGG CAGAGACTCA GAAGCTGGAT 
CTATCTCTGA ACCATACCTT GGCACTAATA TCTTTGGACT GCCGATCTCC TGAGAGACAG 
CTGTTGCACT CAGCAGTGAG ATCCACCACA AGGGATGGTC TGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024998099.1
CDS	505..7812
Translation	MRAREELELKESEWRSEREFYDSYLRGEHNRLLSLWRQVLTFRRHFLEMKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVTLLQQQLAQERKYKQDYIECCAKTSQELLDLHQELSYSLEAVIREPKAAVLEAETQKLDLSLNHTLALISLDCRSPERQLLHSAVRSTTRDGLR

Target ORF information:

RefSeq Version	XM_025142331.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025142331.1

ORF Insert Sequence:

ATGAGGGCCC GAGAGGAGCT GGAATTGAAG GAGAGTGAAT GGCGCAGTGA GCGTGAGTTT 
TATGACAGCT ACTTAAGGGG TGAACATAAC CGTCTTCTGA GCCTATGGCG TCAGGTGCTG 
ACTTTCCGCC GTCACTTCCT GGAAATGAAG ACTGCCACTG ACAGAGATTT GTCAGAGCTG 
AAGGCAGAGC AAATGAGACT CTCTGGATCT ATACTTGTAA GCTGCTTCCG TTTGAACTCT 
GGCGTACAGC TCTGGGAGTC TGTCACTTTG GGGAGGCCTG TCCTAAAGGA TCAAGCACAG 
CAGCAAGTGG AAAGGGAAAT AAACCAGGAG GCTTTGGAAG TGATGTGCTT ACAAGTTGAT 
GAGGACCTGG AGAAGAAAGA GCTTCAAGAT AGGGTGATGG AGCTCTCAGC CTTGCTTGTA 
CAGTCTCAGA AACAAAATGA AGAGAAAGAG AAGACCGTGA AAACACTTAA CGACACTGTG 
GAGATGCTAG AAGCAAGTCG GTTAGAGGTA GAATACGAAG CTTCATTAAC TAAAAATGCC 
AAAGAAGAGA ACCTTGCCCT TCAGAAGCTA ATCAAAGAGA TAACTGAGGT GGTGTTAGAT 
GACAGTGACA GCACAGTCGG CATTGTCCAG CGTCCAGAGT CTAGCAACGT CCTCTCTTGT 
CTGAGCTCCC TTGATGCAGA GCATGCCTTT GTGTTGGTTC AGGAAACACT GGCAAGGAGG 
CAAAAAGCAA TACAGGCCCT AAAAGAAGAG CTTTCTACCA GGCAAGACTC TATCAGTTTT 
CTGCAGCACC AGCACAGACA GCAGGAAGAG AAGTGCAAGA AGCTGCAGCA AAGGCTTGAG 
CAACTGGAGG AGGAAAACAA GATGTCTAAT AGCCACCAGC AGCACCTCAA GTCTCTGGTA 
CTAGCGCTCA AGTGTGACTG TGAAAACCTA GAGAAAATCA GAGGAGAACT ACAGCAAAAG 
CTGGAATTAA GTGAGCAAGA AGCCTCACGT CTGCGTCAGA GTAACACTGA GCTGCAGCTG 
AAGGAAGATT CAGCCCAAGG GGAAAAGGTG GAGCAGCAGC TGGCAATGGA GAGAGCACAT 
CATGACCATG AGCTCGTTCT GAAGGACTTA GCTGCACTTG AAGAAAAACA TTCGTTGTTA 
CAGAATGAAT TGGTAGCCGC GAGGGAGAAG CTGGAGGAAT CACACCTTCA GAGGGATCTG 
CTGAAGCAAG AGAAACATGA GCTTACAGTG GCACTGGAAA AGGCAGAGAA ATCAGTAGCA 
GCATTGACAG GGGCTCAGAA TAAGTTGAAT TCTGAAATAG CTGACCTACA TACTGCTGCA 
GCAAAGATGA GCAGTATTAA TGAAGCTCTT GCACTGGATA AAGTGGAACT GAACAAGCTT 
GTGTTGCAGC TGGAACAAGA GAATGAACTT CTGTCAGATA AAGTGAATGA GATGGAGAGA 
GCAAAGGTCT CTGAACAGGA GAAGATAACC TTGTATAAAA GAACAAATGA AGAGCTTTGT 
GCAGAGAAAT CCCACCTGGA GAAGCTGCTG AAGAAAGCAG AGGAGCAACA GGAAGAGCTG 
CAGATGCAAC TGAGAATACT GGCAGAGGAA AAGGAAGAAA CCCTGGAGAA ACTCAACCAG 
GTTTACCGCC AGCAAGAGTC AGCTAGCAGT GGTTTGGAGC AGTTGCGCCA ACAGTCCTCT 
CGCCAAGAGG ATGCAGTGGC CAAGGTGTCC AAAGAGAAGG AATTCCTGGT GCATGAGAAG 
GCCGCCCTTG AGGTGCGACT GGCAGCTGTG GAACGGGACA GACAAGGCCT TTCAGAGCAG 
CTGGCAGAGG CCAGGTCAGT GAAGGACACC CTGGAATCCA GCCTGTTTGA GGCTCAGCAG 
CAGTTATCTC ACCTGGAGAT CACCAAGAGT CAGCTTGAAA TCCAGCTTCA TACAGTCGTT 
CAGGCTAAGG AAGTGATACA AGGGGAAGTG AAGTGTCTTC AATATGAGCT GGAAACAGAG 
AGATGTCTAA TGAAGCAGGA ACAGAAAAAC ATGGCACAAC GGCTCATTCA GATAGAAGAG 
CAGCATAACA ATACCCTGAA ACTTCAGCAA ACTGATCATG AAGTGGAAAT AAACAAACTC 
CTACAAGACC TGGCAAGTGA ACGGGAGGGG CGCCAGTTAG AGCTACAGGA GATGCTGGAG 
CTCTGGGAAA AGGAGAAAGC AGAGGCAGAA GGGCAGCATG AGAAGAAGCT GTTTCATATG 
AAGGAGAAAG TTGCTACCAT GCAAGCTCAA CAAGAAGAGG AACGAACCAG AGTTGAAAGT 
GCCAATCAAG AGATTTTGAC AGAAAAGGAG AACGAGAAGA AAGCTTTATT AGAGACACTA 
CTCCAAACTC AGGGAGAACT AACAGAAGCC TGCCATCAGC TTGAGCAACT TAGGCAGGAG 
GTGAAAGAGC AGCAGGAGTA TGAGCAGAAC ATCACAGAAA AACTGCAAGC AGAGCTACAG 
GAAACTCACT GTAAAATCAA GATGGTGGAG AATATGCACA AGGAAGAAAT GGAAAACATC 
AAAGAAGAAA AGAATATCCT TCTCCAGCAG AGGGATGATC TACAAAAACA GGTAGAAGAG 
CTGACATCTC AGCTAGCAGC CTCCGAAGAG TCCCATCAAG CAATTGGCCA TAAAGCTCAG 
CAAGAGCTGA GTGAGGCACA AGAGTTGTCC AGGCAGAAGG TATTGGAGGT TGTGCACCTT 
CAGAAAATCT TGGAGGAGGA GAGGGGCCAA TGGGAAGAGG TGGAACATCA GAACAAAGAG 
CTGCAGGTGT GCCTGCAGTC CTTAGAGGGG GAAAGGAGTC GATGGGAAGA AGTGGAACGT 
CACAACACAG AATTTCAGGC TTCATTGAAG GCCCTAGAGA GCGAAAGAGA AAGGCTATCT 
CTGTCTCTGG AACAGAAGGA ATTGAGCCTC AAGACCTTGG AAGAAAACAA CCTTGTCCAG 
CAGAACGAGG TGTCTAAACT ACATTCTGCT ATTCAACAGG CTCAGCAGCT CCACTCAGAC 
CACAGAAGAG AAATACAAGA GCTCAACAAC CAGGTACAGA CACTACAGGA AGTGGTGCTG 
GAGAAGGAAG CTAGCCTGGC AGCTCGGGAG AAGCAGCTAT TACAGGATCT GGAGGAGTCT 
CGAGCAGGCG AACGGTGCTT GAGAGACTCT CTGCACGTGC TAGAGGCTGA GATGGCTGAA 
CTTCATCTGA GGCTTTGTAG CACAGAGAAC AGAGCAAAGG CATTGGCTAC AGAGTGCCAG 
CAGGCTAATA ATGCCCACTG TGAAGCCCAG TCTCAGCTGG ACAAATTGCA CTTGGTTCTT 
CACCACGTGC TGTGTGACAG CACTGATAAA AGCAGAGACT TAGTCGCTTG GAGTTCAGAA 
CAGGATCATG TCTGGGGCCT AACTGTTTCT CAGGCCAAGG ATCTCCATGT AGAGCTCACA 
GTGGACAGAG TGGCAGCAGC CCTGCAAGAC CTGCGTCAGG ACCTGAAGCA AACTCAGCAA 
GATCTGAATG ATGCTGGGAA GAAAGTGCAG GACCTGGAAC TGGAGCTGAG CAAGAGGCAA 
GCTGAGAGGG ACCACTTCAG TGCCCACAAT CAGGAGCTAC AGAAACAGTT GGCTCAAAGC 
CAGGAAGAGA CACAGATGGT AGAAGGGAAG AAGAACTCTC TACAAGCAGC CTTGCAGGCA 
GAAGCTGCTG CTCTAAAGAA GGAGGCTATT ACACTACATC AAGAGGTGGC ATCTTTGGAA 
AGGAAACTTG AGAGCGCTGA GAAGCAAAAA AAGGATGTGC TGCATGAACG GGATAGACTG 
CAAGCAGTTG AAGAAAAACT GATGTGGGAG ATAAAAATTC TTCAGGAGTC AGTCACAGCC 
TCAGAAACAC GAGCAAACAC AGCAGCAGAT ATGAATCACT CCCTTGAACA GGAACTACAA 
ACTACATTGT CTGTCTTGAA AACAAAAAAT AAGGAAGTGG ATGCTCAATG GGAGAAAATC 
CAGATGCTAC AGAAAGAGGC AGCAGATGTA AAAGCTTTGC AGGAGAATCT TATTCAAGTG 
AATGCCATCC TGTCAAAGAG AGAGGGAGAA ATGAAGTTAT ACCAGGAACA GATGAGAATG 
CTGGAAAACC AGAAAGAAAT GCATAAAACT ACTCTAGATC AAGTTATTAA GGACATAAAA 
GAGAAAAAAG AGAAGACAGA ATCCCAGCAA GAACAAATAC AGGAGTTAGA GAAGCAGCAA 
GAACTGCAAA GGACTGTTAT AAGCAAAATG AGCAAAGACC TGGAAGACAG AGATAAGGAG 
ATCAGATCCC AGCAAGAAGA AATATGGGAG CTGGAGAAGC AACAAGAACT GCAGAGGACT 
GTTGTAAGCA AGATGACTAA AGACCTGGCA CACAGAGACC AGGAGATCCA ATCCCAACAA 
GAAGAGATTC AGGAGCTAGA GAAGGAGCGA GAATTGCAGA GGACTGCTGC AAGTAAGATG 
AGCAAAGATC TAAAGGAGAG AGATGAGAAG ATAAGATCCC AGCAAGAACT GATAGAGGAG 
CTAGAGAAGC AGCAAGAACT GCAGAGGACT GCCTTAAGCA AAATGAGCAA AAACCTGGAG 
GAGAGAGACC AGGAGATCAA ATCCCAGCAA GAACTGATAG AGGAGCTAAA GAAGCAACAA 
GAACTACAGA GGACTGCTGT GAGCAAAATG AACAAAGACC TGGAGGAGAG AGACCAGGAG 
ATTAGATCCC AGCAAGAAGA GATACAGGAA CTGGAAAAGC AACGAGAACT GCAGAGGACT 
ATTTTAAGCA AGATGAGCAA AGATCTGGAA GAGAAAGATC AGGTGATCAA GTTCCAGGAA 
GGGAAGGTAA TGATTCTAGA ACAACATGGT ACATCACAGG TGAGAAGTTT GCTAGTGGAT 
CTTGATCATA TGAAGGGAAA CTTGAAGGAG AAAAACTTAG AGCTGATGTC TCTAAATCAG 
CAGATCAAAG AACTGGAGAT GGAGAGAGAA GAGGTGAAAT CTTTGCACAC AAGCCTTGAA 
CAGCTTAGAG CAGTTCTTAG GGATAGAGAG AATGAGTCTG ACTCTCAAAG GGATCAGTTA 
AGACTCTTTC AGCAGTACAA GGAGCATCAA GAGGAGTACC TGCAAGAGCT CCAGGATAAA 
GTAGAAAAAA TGACACTTTC CTTATCTAAA AAAGATCAAG AGCTCGAGTC ACAGCAAAAG 
CAAATCCAGG AAGCTGAAGA AGTCATGGAA AAGAAGTTGA AGACTGTTTG TGACCAACTG 
GAGCAGACAT TAGAAACCTT AAAAGAAAAG GAAAGACTCC TAGACATCCA AAAGCAACAA 
ACAAGGGAAT ATGAGGAAAA AACAGAACAG ATGAATGTCT TATGTAGAGA CTTAGAATAC 
ACTAAGGCAA TATTGAGAGA GAAGGATTTA ATGATTGAAT CTCAGAAGGA ACTGATTGAG 
ACCTTCCAAA AACAAGAAGA CGACTCTATG CAGCAGAAAG AAATTCTGCA GCATCTTAAA 
GGGGCTTTAA AGGAACAAGA ACAAGAAACT TTATCTCTTA GAAAGCAGTG TGAGGCATTT 
AAAGAAAAGG AGGAAAAGCA CAAAACTGAT CAAGTGATTC TTCAAGAAAC AAAAATCACT 
CTGAAAGAAA GAGAAAGAAA GATAGAGGTT TTAGAGAAAG CTGTCTCTAA GCTTCAACAA 
CAAAATGATG ATACGATGAT GCAGACTACA GCTATAGTGC AAAAACTACA ATGTGCTGAA 
TCTTCCTTAG CAGCTAGGGA TCAAGAGATA GCATCTCTGA AAGAGCATGT CCAGGAACTT 
CAGAAGCAGA AGGAGTCGGA AGCTAAACAG GTCAAAAGTC TACAGCAGGA TCTAAACAAA 
ATGAGCAAAA CAATGAAGAA GAACAATTTG CAGATTCTCA AACAGTCGGA GCAGATTAAT 
GTGTTTCATA TTTGTGAAGA AAGTAGGAAA GTAGCACTAA CATCTTGCCA GAATCAAGTG 
AGTCTGCTTG AGGAAGTGGT GAGAAAGAGA GATGAAGACA ATGAAACTCT TCTGCAAAAA 
CTCCAGCACC AAGAAGAAGA ACTGAAGACC TTGCAGAATC TCCAGCTTAT GCTATCTGAG 
AAGAAAGAGG AGATTAAACG TCACGGAGAG GAAGAGAAGT TCCCAGAAGA AGCCTTGCAT 
GGGAGTGAAT GGGAGACGAA GGCTCAAGGT GAGCAAAAAA AGTCAGAAGA TGAGGAAATA 
AGAGGTCTTC ATGAAGATCT CCAGTATGTT CAGCAGACTC TGACAAAGAA GGATGAAGAG 
ATCAAATACC AGAGAGACAG AGTCAAGTAT TTAGAGAAAA CTCTGGCAGG GAAAGAAAAA 
GAGCTGAGGA GGCAGAGTGA ACTCTTGAAA CAATTAACAT CAGCTCTGCA TTGGAAAGAT 
GAAGGGGAGA CTCTAAAGAA ACAAATCCAG AAACTCCAAA AATGGGAGGA AGAAGAAGCA 
GAGAACAGGA GGGTTCTCCA AGAGAGAGAC CAATTCTTGC AAAGGCAGAA GGAACTAGCC 
CAACAACTGG AAGATGAGAG GAAAGTAAAG GGGGAAGAAC TGAAGCGTGT GATTGCTATT 
TTGAAGCAGA CTGAAAGTGG CGAAATCGAA TGGAAAGAGA AGGCACAAGC ACTGACTCTT 
GCCCTTTCTA AGAGTGAAAT GACCAATGGG ACTTTGAGGG AAGAAATAGC CATTCTGCAG 
AGTATGGTTT CAGAGAGGGA CAAGGACCGG TTTCATCTTC AGGAGGCTTT TGAAGGCGGT 
GAGAACCTAT CGTGGCTCTT GGAAAAGAAG CTCCTATGGC AGCGGCTGGA GTGTCTGCAG 
CAAGCAGTTG CAAGGCTGGA ACATGAAAAG TCTGAGCTGA AGCAACTCAA TGTTGAGCTC 
AAGATAACTC TTGAGCAGGT GGAACGTGAA CGGAGGAGGC TGAAGAGAAG TTGTAGTCAT 
CAGTCACAGC CAAATGCAAA CAGGTTTTCT CTCTCGGAAT CTGATGAGGA CAAGATGCCT 
GCTTCTAGAA AGGAGGAGGC TCGTGCTCTC TGCAGTCAAT TAGCTGAGTT ACAGAACCAG 
GTGACTCTTC TGCAGCAGCA GTTGGCACAA GAACGAAAAT ACAAGCAGGA CTATATTGAG 
TGTTGTGCAA AAACCAGTCA GGAGCTTTTG GACCTTCACC AAGAGCTGTC TTACTCCCTG 
GAGGCTGTGA TCAGGGAGCC CAAAGCTGCT GTTCTGGAGG CAGAGACTCA GAAGCTGGAT 
CTATCTCTGA ACCATACCTT GGCACTAATA TCTTTGGACT GCCGATCTCC TGAGAGACAG 
CTGTTGCACT CAGCAGTGAG ATCCACCACA AGGGATGGTC TGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_004946945.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947002.1 Gallus gallus centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

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CloneID	OGa17583
Clone ID Related Accession (Same CDS sequence)	XM_004946945.3 , XM_004946945.2
Accession Version	XM_004946945.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC AGTATCGGAG CTGGTGTCGT GAGCTGGAGC AGCGACTTGA AGCAGGAGGG 
GTGAGTGTGC AGGGATCCCT TTCATACAGA TGGGAAACCA CTGAAGACCA CAGCCTGGAG 
AAGGCACTCC TCCAGTTAGA AGAAGAGCAG CAAAGGTGTG AGAATCTGGC AGAAGTGAAT 
ACCCTCCTGC GGGAACACCT AGATAAAGCA AATGAGGTGA ATTTAGCCCT TAAAGAAGAT 
GTTGGAAAAC TGACAGCAGA TTGGATGAGG GCCCGAGAGG AGCTGGAATT GAAGGAGAGT 
GAATGGCGCA GTGAGCGTGA GTTTTATGAC AGCTACTTAA GGGGTGAACA TAACCGTCTT 
CTGAGCCTAT GGCGTCAGGT GCTGACTTTC CGCCGTCACT TCCTGGAAAT GAAGACTGCC 
ACTGACAGAG ATTTGTCAGA GCTGAAGGCA GAGCAAATGA GACTCTCTGG ATCTATACTT 
GTAAGCTGCT TCCGTTTGAA CTCTGGCGTA CAGCTCTGGG AGTCTGTCAC TTTGGGGAGG 
CCTGTCCTAA AGGATCAAGC ACAGCAGCAA GTGGAAAGGG AAATAAACCA GGAGGCTTTG 
GAAGTGATGT GCTTACAAGT TGATGAGGAC CTGGAGAAGA AAGAGCTTCA AGATAGGGTG 
ATGGAGCTCT CAGCCTTGCT TGTACAGTCT CAGAAACAAA ATGAAGAGAA AGAGAAGACC 
GTGAAAACAC TTAACGACAC TGTGGAGATG CTAGAAGCAA GTCGGTTAGA GGTAGAATAC 
GAAGCTTCAT TAACTAAAAA TGCCAAAGAA GAGAACCTTG CCCTTCAGAA GCTAATCAAA 
GAGATAACTG AGGTGGTGTT AGATGACAGT GACAGCACAG TCGGCATTGT CCAGCGTCCA 
GAGTCTAGCA ACGTCCTCTC TTGTCTGAGC TCCCTTGATG CAGAGCATGC CTTTGTGTTG 
GTTCAGGAAA CACTGGCAAG GAGGCAAAAA GCAATACAGG CCCTAAAAGA AGAGCTTTCT 
ACCAGGCAAG ACTCTATCAG TTTTCTGCAG CACCAGCACA GACAGCAGGA AGAGAAGTGC 
AAGAAGCTGC AGCAAAGGCT TGAGCAACTG GAGGAGGAAA ACAAGATGTC TAATAGCCAC 
CAGCAGCACC TCAAGTCTCT GGTACTAGCG CTCAAGTGTG ACTGTGAAAA CCTAGAGAAA 
ATCAGAGGAG AACTACAGCA AAAGCTGGAA TTAAGTGAGC AAGAAGCCTC ACGTCTGCGT 
CAGAGTAACA CTGAGCTGCA GCTGAAGGAA GATTCAGCCC AAGGGGAAAA GGTGGAGCAG 
CAGCTGGCAA TGGAGAGAGC ACATCATGAC CATGAGCTCG TTCTGAAGGA CTTAGCTGCA 
CTTGAAGAAA AACATTCGTT GTTACAGAAT GAATTGGTAG CCGCGAGGGA GAAGCTGGAG 
GAATCACACC TTCAGAGGGA TCTGCTGAAG CAAGAGAAAC ATGAGCTTAC AGTGGCACTG 
GAAAAGGCAG AGAAATCAGT AGCAGCATTG ACAGGGGCTC AGAATAAGTT GAATTCTGAA 
ATAGCTGACC TACATACTGC TGCAGCAAAG ATGAGCAGTA TTAATGAAGC TCTTGCACTG 
GATAAAGTGG AACTGAACAA GCTTGTGTTG CAGCTGGAAC AAGAGAATGA ACTTCTGTCA 
GATAAAGTGA ATGAGATGGA GAGAGCAAAG GTCTCTGAAC AGGAGAAGAT AACCTTGTAT 
AAAAGAACAA ATGAAGAGCT TTGTGCAGAG AAATCCCACC TGGAGAAGCT GCTGAAGAAA 
GCAGAGGAGC AACAGGAAGA GCTGCAGATG CAACTGAGAA TACTGGCAGA GGAAAAGGAA 
GAAACCCTGG AGAAACTCAA CCAGGTTTAC CGCCAGCAAG AGTCAGCTAG CAGTGGTTTG 
GAGCAGTTGC GCCAACAGTC CTCTCGCCAA GAGGATGCAG TGGCCAAGGT GTCCAAAGAG 
AAGGAATTCC TGGTGCATGA GAAGGCCGCC CTTGAGGTGC GACTGGCAGC TGTGGAACGG 
GACAGACAAG GCCTTTCAGA GCAGCTGGCA GAGGCCAGGT CAGTGAAGGA CACCCTGGAA 
TCCAGCCTGT TTGAGGCTCA GCAGCAGTTA TCTCACCTGG AGATCACCAA GAGTCAGCTT 
GAAATCCAGC TTCATACAGT CGTTCAGGCT AAGGAAGTGA TACAAGGGGA AGTGAAGTGT 
CTTCAATATG AGCTGGAAAC AGAGAGATGT CTAATGAAGC AGGAACAGAA AAACATGGCA 
CAACGGCTCA TTCAGATAGA AGAGCAGCAT AACAATACCC TGAAACTTCA GCAAACTGAT 
CATGAAGTGG AAATAAACAA ACTCCTACAA GACCTGGCAA GTGAACGGGA GGGGCGCCAG 
TTAGAGCTAC AGGAGATGCT GGAGCTCTGG GAAAAGGAGA AAGCAGAGGC AGAAGGGCAG 
CATGAGAAGA AGCTGTTTCA TATGAAGGAG AAAGTTGCTA CCATGCAAGC TCAACAAGAA 
GAGGAACGAA CCAGAGTTGA AAGTGCCAAT CAAGAGATTT TGACAGAAAA GGAGAACGAG 
AAGAAAGCTT TATTAGAGAC ACTACTCCAA ACTCAGGGAG AACTAACAGA AGCCTGCCAT 
CAGCTTGAGC AACTTAGGCA GGAGGTGAAA GAGCAGCAGG AGTATGAGCA GAACATCACA 
GAAAAACTGC AAGCAGAGCT ACAGGAAACT CACTGTAAAA TCAAGATGGT GGAGAATATG 
CACAAGGAAG AAATGGAAAA CATCAAAGAA GAAAAGAATA TCCTTCTCCA GCAGAGGGAT 
GATCTACAAA AACAGGTAGA AGAGCTGACA TCTCAGCTAG CAGCCTCCGA AGAGTCCCAT 
CAAGCAATTG GCCATAAAGC TCAGCAAGAG CTGAGTGAGG CACAAGAGTT GTCCAGGCAG 
AAGGTATTGG AGGTTGTGCA CCTTCAGAAA ATCTTGGAGG AGGAGAGGGG CCAATGGGAA 
GAGGTGGAAC ATCAGAACAA AGAGCTGCAG GTGTGCCTGC AGTCCTTAGA GGGGGAAAGG 
AGTCGATGGG AAGAAGTGGA ACGTCACAAC ACAGAATTTC AGGCTTCATT GAAGGCCCTA 
GAGAGCGAAA GAGAAAGGCT ATCTCTGTCT CTGGAACAGA AGGAATTGAG CCTCAAGACC 
TTGGAAGAAA ACAACCTTGT CCAGCAGAAC GAGGTGTCTA AACTACATTC TGCTATTCAA 
CAGGCTCAGC AGCTCCACTC AGACCACAGA AGAGAAATAC AAGAGCTCAA CAACCAGGTA 
CAGACACTAC AGGAAGTGGT GCTGGAGAAG GAAGCTAGCC TGGCAGCTCG GGAGAAGCAG 
CTATTACAGG ATCTGGAGGA GTCTCGAGCA GGCGAACGGT GCTTGAGAGA CTCTCTGCAC 
GTGCTAGAGG CTGAGATGGC TGAACTTCAT CTGAGGCTTT GTAGCACAGA GAACAGAGCA 
AAGGCATTGG CTACAGAGTG CCAGCAGGCT AATAATGCCC ACTGTGAAGC CCAGTCTCAG 
CTGGACAAAT TGCACTTGGT TCTTCACCAC GTGCTGTGTG ACAGCACTGA TAAAAGCAGA 
GACTTAGTCG CTTGGAGTTC AGAACAGGAT CATGTCTGGG GCCTAACTGT TTCTCAGGCC 
AAGGATCTCC ATGTAGAGCT CACAGTGGAC AGAGTGGCAG CAGCCCTGCA AGACCTGCGT 
CAGGACCTGA AGCAAACTCA GCAAGATCTG AATGATGCTG GGAAGAAAGT GCAGGACCTG 
GAACTGGAGC TGAGCAAGAG GCAAGCTGAG AGGGACCACT TCAGTGCCCA CAATCAGGAG 
CTACAGAAAC AGTTGGCTCA AAGCCAGGAA GAGACACAGA TGGTAGAAGG GAAGAAGAAC 
TCTCTACAAG CAGCCTTGCA GGCAGAAGCT GCTGCTCTAA AGAAGGAGGC TATTACACTA 
CATCAAGAGG TGGCATCTTT GGAAAGGAAA CTTGAGAGCG CTGAGAAGCA AAAAAAGGAT 
GTGCTGCATG AACGGGATAG ACTGCAAGCA GTTGAAGAAA AACTGATGTG GGAGATAAAA 
ATTCTTCAGG AGTCAGTCAC AGCCTCAGAA ACACGAGCAA ACACAGCAGC AGATATGAAT 
CACTCCCTTG AACAGGAACT ACAAACTACA TTGTCTGTCT TGAAAACAAA AAATAAGGAA 
GTGGATGCTC AATGGGAGAA AATCCAGATG CTACAGAAAG AGGCAGCAGA TGTAAAAGCT 
TTGCAGGAGA ATCTTATTCA AGTGAATGCC ATCCTGTCAA AGAGAGAGGG AGAAATGAAG 
TTATACCAGG AACAGATGAG AATGCTGGAA AACCAGAAAG AAATGCATAA AACTACTCTA 
GATCAAGTTA TTAAGGACAT AAAAGAGAAA AAAGAGAAGA CAGAATCCCA GCAAGAACAA 
ATACAGGAGT TAGAGAAGCA GCAAGAACTG CAAAGGACTG TTATAAGCAA AATGAGCAAA 
GACCTGGAAG ACAGAGATAA GGAGATCAGA TCCCAGCAAG AAGAAATATG GGAGCTGGAG 
AAGCAACAAG AACTGCAGAG GACTGTTGTA AGCAAGATGA CTAAAGACCT GGCACACAGA 
GACCAGGAGA TCCAATCCCA ACAAGAAGAG ATTCAGGAGC TAGAGAAGGA GCGAGAATTG 
CAGAGGACTG CTGCAAGTAA GATGAGCAAA GATCTAAAGG AGAGAGATGA GAAGATAAGA 
TCCCAGCAAG AACTGATAGA GGAGCTAGAG AAGCAGCAAG AACTGCAGAG GACTGCCTTA 
AGCAAAATGA GCAAAAACCT GGAGGAGAGA GACCAGGAGA TCAAATCCCA GCAAGAACTG 
ATAGAGGAGC TAAAGAAGCA ACAAGAACTA CAGAGGACTG CTGTGAGCAA AATGAACAAA 
GACCTGGAGG AGAGAGACCA GGAGATTAGA TCCCAGCAAG AAGAGATACA GGAACTGGAA 
AAGCAACGAG AACTGCAGAG GACTATTTTA AGCAAGATGA GCAAAGATCT GGAAGAGAAA 
GATCAGGTGA TCAAGTTCCA GGAAGGGAAG GTAATGATTC TAGAACAACA TGGTACATCA 
CAGGTGAGAA GTTTGCTAGT GGATCTTGAT CATATGAAGG GAAACTTGAA GGAGAAAAAC 
TTAGAGCTGA TGTCTCTAAA TCAGCAGATC AAAGAACTGG AGATGGAGAG AGAAGAGGTG 
AAATCTTTGC ACACAAGCCT TGAACAGCTT AGAGCAGTTC TTAGGGATAG AGAGAATGAG 
TCTGACTCTC AAAGGGATCA GTTAAGACTC TTTCAGCAGT ACAAGGAGCA TCAAGAGGAG 
TACCTGCAAG AGCTCCAGGA TAAAGTAGAA AAAATGACAC TTTCCTTATC TAAAAAAGAT 
CAAGAGCTCG AGTCACAGCA AAAGCAAATC CAGGAAGCTG AAGAAGTCAT GGAAAAGAAG 
TTGAAGACTG TTTGTGACCA ACTGGAGCAG ACATTAGAAA CCTTAAAAGA AAAGGAAAGA 
CTCCTAGACA TCCAAAAGCA ACAAACAAGG GAATATGAGG AAAAAACAGA ACAGATGAAT 
GTCTTATGTA GAGACTTAGA ATACACTAAG GCAATATTGA GAGAGAAGGA TTTAATGATT 
GAATCTCAGA AGGAACTGAT TGAGACCTTC CAAAAACAAG AAGACGACTC TATGCAGCAG 
AAAGAAATTC TGCAGCATCT TAAAGGGGCT TTAAAGGAAC AAGAACAAGA AACTTTATCT 
CTTAGAAAGC AGTGTGAGGC ATTTAAAGAA AAGGAGGAAA AGCACAAAAC TGATCAAGTG 
ATTCTTCAAG AAACAAAAAT CACTCTGAAA GAAAGAGAAA GAAAGATAGA GGTTTTAGAG 
AAAGCTGTCT CTAAGCTTCA ACAACAAAAT GATGATACGA TGATGCAGAC TACAGCTATA 
GTGCAAAAAC TACAATGTGC TGAATCTTCC TTAGCAGCTA GGGATCAAGA GATAGCATCT 
CTGAAAGAGC ATGTCCAGGA ACTTCAGAAG CAGAAGGAGT CGGAAGCTAA ACAGGTCAAA 
AGTCTACAGC AGGATCTAAA CAAAATGAGC AAAACAATGA AGAAGAACAA TTTGCAGATT 
CTCAAACAGT CGGAGCAGAT TAATGTGTTT CATATTTGTG AAGAAAGTAG GAAAGTAGCA 
CTAACATCTT GCCAGAATCA AGTGAGTCTG CTTGAGGAAG TGGTGAGAAA GAGAGATGAA 
GACAATGAAA CTCTTCTGCA AAAACTCCAG CACCAAGAAG AAGAACTGAA GACCTTGCAG 
AATCTCCAGC TTATGCTATC TGAGAAGAAA GAGGAGATTA AACGTCACGG AGAGGAAGAG 
AAGTTCCCAG AAGAAGCCTT GCATGGGAGT GAATGGGAGA CGAAGGCTCA AGGTGAGCAA 
AAAAAGTCAG AAGATGAGGA AATAAGAGGT CTTCATGAAG ATCTCCAGTA TGTTCAGCAG 
ACTCTGACAA AGAAGGATGA AGAGATCAAA TACCAGAGAG ACAGAGTCAA GTATTTAGAG 
AAAACTCTGG CAGGGAAAGA AAAAGAGCTG AGGAGGCAGA GTGAACTCTT GAAACAATTA 
ACATCAGCTC TGCATTGGAA AGATGAAGGG GAGACTCTAA AGAAACAAAT CCAGAAACTC 
CAAAAATGGG AGGAAGAAGA AGCAGAGAAC AGGAGGGTTC TCCAAGAGAG AGACCAATTC 
TTGCAAAGGC AGAAGGAACT AGCCCAACAA CTGGAAGATG AGAGGAAAGT AAAGGGGGAA 
GAACTGAAGC GTGTGATTGC TATTTTGAAG CAGACTGAAA GTGGCGAAAT CGAATGGAAA 
GAGAAGGCAC AAGCACTGAC TCTTGCCCTT TCTAAGAGTG AAATGACCAA TGGGACTTTG 
AGGGAAGAAA TAGCCATTCT GCAGAGTATG GTTTCAGAGA GGGACAAGGA CCGGTTTCAT 
CTTCAGGAGG CTTTTGAAGG CGGTGAGAAC CTATCGTGGC TCTTGGAAAA GAAGCTCCTA 
TGGCAGCGGC TGGAGTGTCT GCAGCAAGCA GTTGCAAGGC TGGAACATGA AAAGTCTGAG 
CTGAAGCAAC TCAATGTTGA GCTCAAGATA ACTCTTGAGC AGGTGGAACG TGAACGGAGG 
AGGCTGAAGA GAAGTTGTAG TCATCAGTCA CAGCCAAATG CAAACAGGTT TTCTCTCTCG 
GAATCTGATG AGGACAAGAT GCCTGCTTCT AGAAAGGAGG AGGCTCGTGC TCTCTGCAGT 
CAATTAGCTG AGTTACAGAA CCAGGTGACT CTTCTGCAGC AGCAGTTGGC ACAAGAACGA 
AAATACAAGC AGGACTATAT TGAGTGTTGT GCAAAAACCA GTCAGGAGCT TTTGGACCTT 
CACCAAGAGC TGTCTTACTC CCTGGAGGCT GTGATCAGGG AGCCCAAAGC TGCTGTTCTG 
GAGGCAGAGA CTCAGAAGCT GGATCTATCT CTGAACCATA CCTTGGCACT AATATCTTTG 
GACTGCCGAT CTCCTGAGAG ACAGCTGTTG CACTCAGCAG TGAGATCCAC CACAAGGGAT 
GGTCTGAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947002.1
CDS	185..7756
Translation	MLLQYRSWCRELEQRLEAGGVSVQGSLSYRWETTEDHSLEKALLQLEEEQQRCENLAEVNTLLREHLDKANEVNLALKEDVGKLTADWMRAREELELKESEWRSEREFYDSYLRGEHNRLLSLWRQVLTFRRHFLEMKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVTLLQQQLAQERKYKQDYIECCAKTSQELLDLHQELSYSLEAVIREPKAAVLEAETQKLDLSLNHTLALISLDCRSPERQLLHSAVRSTTRDGLR

Target ORF information:

RefSeq Version	XM_004946945.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946945.3

ORF Insert Sequence:

ATGCTGCTGC AGTATCGGAG CTGGTGTCGT GAGCTGGAGC AGCGACTTGA AGCAGGAGGG 
GTGAGTGTGC AGGGATCCCT TTCATACAGA TGGGAAACCA CTGAAGACCA CAGCCTGGAG 
AAGGCACTCC TCCAGTTAGA AGAAGAGCAG CAAAGGTGTG AGAATCTGGC AGAAGTGAAT 
ACCCTCCTGC GGGAACACCT AGATAAAGCA AATGAGGTGA ATTTAGCCCT TAAAGAAGAT 
GTTGGAAAAC TGACAGCAGA TTGGATGAGG GCCCGAGAGG AGCTGGAATT GAAGGAGAGT 
GAATGGCGCA GTGAGCGTGA GTTTTATGAC AGCTACTTAA GGGGTGAACA TAACCGTCTT 
CTGAGCCTAT GGCGTCAGGT GCTGACTTTC CGCCGTCACT TCCTGGAAAT GAAGACTGCC 
ACTGACAGAG ATTTGTCAGA GCTGAAGGCA GAGCAAATGA GACTCTCTGG ATCTATACTT 
GTAAGCTGCT TCCGTTTGAA CTCTGGCGTA CAGCTCTGGG AGTCTGTCAC TTTGGGGAGG 
CCTGTCCTAA AGGATCAAGC ACAGCAGCAA GTGGAAAGGG AAATAAACCA GGAGGCTTTG 
GAAGTGATGT GCTTACAAGT TGATGAGGAC CTGGAGAAGA AAGAGCTTCA AGATAGGGTG 
ATGGAGCTCT CAGCCTTGCT TGTACAGTCT CAGAAACAAA ATGAAGAGAA AGAGAAGACC 
GTGAAAACAC TTAACGACAC TGTGGAGATG CTAGAAGCAA GTCGGTTAGA GGTAGAATAC 
GAAGCTTCAT TAACTAAAAA TGCCAAAGAA GAGAACCTTG CCCTTCAGAA GCTAATCAAA 
GAGATAACTG AGGTGGTGTT AGATGACAGT GACAGCACAG TCGGCATTGT CCAGCGTCCA 
GAGTCTAGCA ACGTCCTCTC TTGTCTGAGC TCCCTTGATG CAGAGCATGC CTTTGTGTTG 
GTTCAGGAAA CACTGGCAAG GAGGCAAAAA GCAATACAGG CCCTAAAAGA AGAGCTTTCT 
ACCAGGCAAG ACTCTATCAG TTTTCTGCAG CACCAGCACA GACAGCAGGA AGAGAAGTGC 
AAGAAGCTGC AGCAAAGGCT TGAGCAACTG GAGGAGGAAA ACAAGATGTC TAATAGCCAC 
CAGCAGCACC TCAAGTCTCT GGTACTAGCG CTCAAGTGTG ACTGTGAAAA CCTAGAGAAA 
ATCAGAGGAG AACTACAGCA AAAGCTGGAA TTAAGTGAGC AAGAAGCCTC ACGTCTGCGT 
CAGAGTAACA CTGAGCTGCA GCTGAAGGAA GATTCAGCCC AAGGGGAAAA GGTGGAGCAG 
CAGCTGGCAA TGGAGAGAGC ACATCATGAC CATGAGCTCG TTCTGAAGGA CTTAGCTGCA 
CTTGAAGAAA AACATTCGTT GTTACAGAAT GAATTGGTAG CCGCGAGGGA GAAGCTGGAG 
GAATCACACC TTCAGAGGGA TCTGCTGAAG CAAGAGAAAC ATGAGCTTAC AGTGGCACTG 
GAAAAGGCAG AGAAATCAGT AGCAGCATTG ACAGGGGCTC AGAATAAGTT GAATTCTGAA 
ATAGCTGACC TACATACTGC TGCAGCAAAG ATGAGCAGTA TTAATGAAGC TCTTGCACTG 
GATAAAGTGG AACTGAACAA GCTTGTGTTG CAGCTGGAAC AAGAGAATGA ACTTCTGTCA 
GATAAAGTGA ATGAGATGGA GAGAGCAAAG GTCTCTGAAC AGGAGAAGAT AACCTTGTAT 
AAAAGAACAA ATGAAGAGCT TTGTGCAGAG AAATCCCACC TGGAGAAGCT GCTGAAGAAA 
GCAGAGGAGC AACAGGAAGA GCTGCAGATG CAACTGAGAA TACTGGCAGA GGAAAAGGAA 
GAAACCCTGG AGAAACTCAA CCAGGTTTAC CGCCAGCAAG AGTCAGCTAG CAGTGGTTTG 
GAGCAGTTGC GCCAACAGTC CTCTCGCCAA GAGGATGCAG TGGCCAAGGT GTCCAAAGAG 
AAGGAATTCC TGGTGCATGA GAAGGCCGCC CTTGAGGTGC GACTGGCAGC TGTGGAACGG 
GACAGACAAG GCCTTTCAGA GCAGCTGGCA GAGGCCAGGT CAGTGAAGGA CACCCTGGAA 
TCCAGCCTGT TTGAGGCTCA GCAGCAGTTA TCTCACCTGG AGATCACCAA GAGTCAGCTT 
GAAATCCAGC TTCATACAGT CGTTCAGGCT AAGGAAGTGA TACAAGGGGA AGTGAAGTGT 
CTTCAATATG AGCTGGAAAC AGAGAGATGT CTAATGAAGC AGGAACAGAA AAACATGGCA 
CAACGGCTCA TTCAGATAGA AGAGCAGCAT AACAATACCC TGAAACTTCA GCAAACTGAT 
CATGAAGTGG AAATAAACAA ACTCCTACAA GACCTGGCAA GTGAACGGGA GGGGCGCCAG 
TTAGAGCTAC AGGAGATGCT GGAGCTCTGG GAAAAGGAGA AAGCAGAGGC AGAAGGGCAG 
CATGAGAAGA AGCTGTTTCA TATGAAGGAG AAAGTTGCTA CCATGCAAGC TCAACAAGAA 
GAGGAACGAA CCAGAGTTGA AAGTGCCAAT CAAGAGATTT TGACAGAAAA GGAGAACGAG 
AAGAAAGCTT TATTAGAGAC ACTACTCCAA ACTCAGGGAG AACTAACAGA AGCCTGCCAT 
CAGCTTGAGC AACTTAGGCA GGAGGTGAAA GAGCAGCAGG AGTATGAGCA GAACATCACA 
GAAAAACTGC AAGCAGAGCT ACAGGAAACT CACTGTAAAA TCAAGATGGT GGAGAATATG 
CACAAGGAAG AAATGGAAAA CATCAAAGAA GAAAAGAATA TCCTTCTCCA GCAGAGGGAT 
GATCTACAAA AACAGGTAGA AGAGCTGACA TCTCAGCTAG CAGCCTCCGA AGAGTCCCAT 
CAAGCAATTG GCCATAAAGC TCAGCAAGAG CTGAGTGAGG CACAAGAGTT GTCCAGGCAG 
AAGGTATTGG AGGTTGTGCA CCTTCAGAAA ATCTTGGAGG AGGAGAGGGG CCAATGGGAA 
GAGGTGGAAC ATCAGAACAA AGAGCTGCAG GTGTGCCTGC AGTCCTTAGA GGGGGAAAGG 
AGTCGATGGG AAGAAGTGGA ACGTCACAAC ACAGAATTTC AGGCTTCATT GAAGGCCCTA 
GAGAGCGAAA GAGAAAGGCT ATCTCTGTCT CTGGAACAGA AGGAATTGAG CCTCAAGACC 
TTGGAAGAAA ACAACCTTGT CCAGCAGAAC GAGGTGTCTA AACTACATTC TGCTATTCAA 
CAGGCTCAGC AGCTCCACTC AGACCACAGA AGAGAAATAC AAGAGCTCAA CAACCAGGTA 
CAGACACTAC AGGAAGTGGT GCTGGAGAAG GAAGCTAGCC TGGCAGCTCG GGAGAAGCAG 
CTATTACAGG ATCTGGAGGA GTCTCGAGCA GGCGAACGGT GCTTGAGAGA CTCTCTGCAC 
GTGCTAGAGG CTGAGATGGC TGAACTTCAT CTGAGGCTTT GTAGCACAGA GAACAGAGCA 
AAGGCATTGG CTACAGAGTG CCAGCAGGCT AATAATGCCC ACTGTGAAGC CCAGTCTCAG 
CTGGACAAAT TGCACTTGGT TCTTCACCAC GTGCTGTGTG ACAGCACTGA TAAAAGCAGA 
GACTTAGTCG CTTGGAGTTC AGAACAGGAT CATGTCTGGG GCCTAACTGT TTCTCAGGCC 
AAGGATCTCC ATGTAGAGCT CACAGTGGAC AGAGTGGCAG CAGCCCTGCA AGACCTGCGT 
CAGGACCTGA AGCAAACTCA GCAAGATCTG AATGATGCTG GGAAGAAAGT GCAGGACCTG 
GAACTGGAGC TGAGCAAGAG GCAAGCTGAG AGGGACCACT TCAGTGCCCA CAATCAGGAG 
CTACAGAAAC AGTTGGCTCA AAGCCAGGAA GAGACACAGA TGGTAGAAGG GAAGAAGAAC 
TCTCTACAAG CAGCCTTGCA GGCAGAAGCT GCTGCTCTAA AGAAGGAGGC TATTACACTA 
CATCAAGAGG TGGCATCTTT GGAAAGGAAA CTTGAGAGCG CTGAGAAGCA AAAAAAGGAT 
GTGCTGCATG AACGGGATAG ACTGCAAGCA GTTGAAGAAA AACTGATGTG GGAGATAAAA 
ATTCTTCAGG AGTCAGTCAC AGCCTCAGAA ACACGAGCAA ACACAGCAGC AGATATGAAT 
CACTCCCTTG AACAGGAACT ACAAACTACA TTGTCTGTCT TGAAAACAAA AAATAAGGAA 
GTGGATGCTC AATGGGAGAA AATCCAGATG CTACAGAAAG AGGCAGCAGA TGTAAAAGCT 
TTGCAGGAGA ATCTTATTCA AGTGAATGCC ATCCTGTCAA AGAGAGAGGG AGAAATGAAG 
TTATACCAGG AACAGATGAG AATGCTGGAA AACCAGAAAG AAATGCATAA AACTACTCTA 
GATCAAGTTA TTAAGGACAT AAAAGAGAAA AAAGAGAAGA CAGAATCCCA GCAAGAACAA 
ATACAGGAGT TAGAGAAGCA GCAAGAACTG CAAAGGACTG TTATAAGCAA AATGAGCAAA 
GACCTGGAAG ACAGAGATAA GGAGATCAGA TCCCAGCAAG AAGAAATATG GGAGCTGGAG 
AAGCAACAAG AACTGCAGAG GACTGTTGTA AGCAAGATGA CTAAAGACCT GGCACACAGA 
GACCAGGAGA TCCAATCCCA ACAAGAAGAG ATTCAGGAGC TAGAGAAGGA GCGAGAATTG 
CAGAGGACTG CTGCAAGTAA GATGAGCAAA GATCTAAAGG AGAGAGATGA GAAGATAAGA 
TCCCAGCAAG AACTGATAGA GGAGCTAGAG AAGCAGCAAG AACTGCAGAG GACTGCCTTA 
AGCAAAATGA GCAAAAACCT GGAGGAGAGA GACCAGGAGA TCAAATCCCA GCAAGAACTG 
ATAGAGGAGC TAAAGAAGCA ACAAGAACTA CAGAGGACTG CTGTGAGCAA AATGAACAAA 
GACCTGGAGG AGAGAGACCA GGAGATTAGA TCCCAGCAAG AAGAGATACA GGAACTGGAA 
AAGCAACGAG AACTGCAGAG GACTATTTTA AGCAAGATGA GCAAAGATCT GGAAGAGAAA 
GATCAGGTGA TCAAGTTCCA GGAAGGGAAG GTAATGATTC TAGAACAACA TGGTACATCA 
CAGGTGAGAA GTTTGCTAGT GGATCTTGAT CATATGAAGG GAAACTTGAA GGAGAAAAAC 
TTAGAGCTGA TGTCTCTAAA TCAGCAGATC AAAGAACTGG AGATGGAGAG AGAAGAGGTG 
AAATCTTTGC ACACAAGCCT TGAACAGCTT AGAGCAGTTC TTAGGGATAG AGAGAATGAG 
TCTGACTCTC AAAGGGATCA GTTAAGACTC TTTCAGCAGT ACAAGGAGCA TCAAGAGGAG 
TACCTGCAAG AGCTCCAGGA TAAAGTAGAA AAAATGACAC TTTCCTTATC TAAAAAAGAT 
CAAGAGCTCG AGTCACAGCA AAAGCAAATC CAGGAAGCTG AAGAAGTCAT GGAAAAGAAG 
TTGAAGACTG TTTGTGACCA ACTGGAGCAG ACATTAGAAA CCTTAAAAGA AAAGGAAAGA 
CTCCTAGACA TCCAAAAGCA ACAAACAAGG GAATATGAGG AAAAAACAGA ACAGATGAAT 
GTCTTATGTA GAGACTTAGA ATACACTAAG GCAATATTGA GAGAGAAGGA TTTAATGATT 
GAATCTCAGA AGGAACTGAT TGAGACCTTC CAAAAACAAG AAGACGACTC TATGCAGCAG 
AAAGAAATTC TGCAGCATCT TAAAGGGGCT TTAAAGGAAC AAGAACAAGA AACTTTATCT 
CTTAGAAAGC AGTGTGAGGC ATTTAAAGAA AAGGAGGAAA AGCACAAAAC TGATCAAGTG 
ATTCTTCAAG AAACAAAAAT CACTCTGAAA GAAAGAGAAA GAAAGATAGA GGTTTTAGAG 
AAAGCTGTCT CTAAGCTTCA ACAACAAAAT GATGATACGA TGATGCAGAC TACAGCTATA 
GTGCAAAAAC TACAATGTGC TGAATCTTCC TTAGCAGCTA GGGATCAAGA GATAGCATCT 
CTGAAAGAGC ATGTCCAGGA ACTTCAGAAG CAGAAGGAGT CGGAAGCTAA ACAGGTCAAA 
AGTCTACAGC AGGATCTAAA CAAAATGAGC AAAACAATGA AGAAGAACAA TTTGCAGATT 
CTCAAACAGT CGGAGCAGAT TAATGTGTTT CATATTTGTG AAGAAAGTAG GAAAGTAGCA 
CTAACATCTT GCCAGAATCA AGTGAGTCTG CTTGAGGAAG TGGTGAGAAA GAGAGATGAA 
GACAATGAAA CTCTTCTGCA AAAACTCCAG CACCAAGAAG AAGAACTGAA GACCTTGCAG 
AATCTCCAGC TTATGCTATC TGAGAAGAAA GAGGAGATTA AACGTCACGG AGAGGAAGAG 
AAGTTCCCAG AAGAAGCCTT GCATGGGAGT GAATGGGAGA CGAAGGCTCA AGGTGAGCAA 
AAAAAGTCAG AAGATGAGGA AATAAGAGGT CTTCATGAAG ATCTCCAGTA TGTTCAGCAG 
ACTCTGACAA AGAAGGATGA AGAGATCAAA TACCAGAGAG ACAGAGTCAA GTATTTAGAG 
AAAACTCTGG CAGGGAAAGA AAAAGAGCTG AGGAGGCAGA GTGAACTCTT GAAACAATTA 
ACATCAGCTC TGCATTGGAA AGATGAAGGG GAGACTCTAA AGAAACAAAT CCAGAAACTC 
CAAAAATGGG AGGAAGAAGA AGCAGAGAAC AGGAGGGTTC TCCAAGAGAG AGACCAATTC 
TTGCAAAGGC AGAAGGAACT AGCCCAACAA CTGGAAGATG AGAGGAAAGT AAAGGGGGAA 
GAACTGAAGC GTGTGATTGC TATTTTGAAG CAGACTGAAA GTGGCGAAAT CGAATGGAAA 
GAGAAGGCAC AAGCACTGAC TCTTGCCCTT TCTAAGAGTG AAATGACCAA TGGGACTTTG 
AGGGAAGAAA TAGCCATTCT GCAGAGTATG GTTTCAGAGA GGGACAAGGA CCGGTTTCAT 
CTTCAGGAGG CTTTTGAAGG CGGTGAGAAC CTATCGTGGC TCTTGGAAAA GAAGCTCCTA 
TGGCAGCGGC TGGAGTGTCT GCAGCAAGCA GTTGCAAGGC TGGAACATGA AAAGTCTGAG 
CTGAAGCAAC TCAATGTTGA GCTCAAGATA ACTCTTGAGC AGGTGGAACG TGAACGGAGG 
AGGCTGAAGA GAAGTTGTAG TCATCAGTCA CAGCCAAATG CAAACAGGTT TTCTCTCTCG 
GAATCTGATG AGGACAAGAT GCCTGCTTCT AGAAAGGAGG AGGCTCGTGC TCTCTGCAGT 
CAATTAGCTG AGTTACAGAA CCAGGTGACT CTTCTGCAGC AGCAGTTGGC ACAAGAACGA 
AAATACAAGC AGGACTATAT TGAGTGTTGT GCAAAAACCA GTCAGGAGCT TTTGGACCTT 
CACCAAGAGC TGTCTTACTC CCTGGAGGCT GTGATCAGGG AGCCCAAAGC TGCTGTTCTG 
GAGGCAGAGA CTCAGAAGCT GGATCTATCT CTGAACCATA CCTTGGCACT AATATCTTTG 
GACTGCCGAT CTCCTGAGAG ACAGCTGTTG CACTCAGCAG TGAGATCCAC CACAAGGGAT 
GGTCTGAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_004946945.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947002.1 Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

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CloneID	OGa17583
Clone ID Related Accession (Same CDS sequence)	XM_004946945.3 , XM_004946945.2
Accession Version	XM_004946945.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455990.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946945.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC AGTATCGGAG CTGGTGTCGT GAGCTGGAGC AGCGACTTGA AGCAGGAGGG 
GTGAGTGTGC AGGGATCCCT TTCATACAGA TGGGAAACCA CTGAAGACCA CAGCCTGGAG 
AAGGCACTCC TCCAGTTAGA AGAAGAGCAG CAAAGGTGTG AGAATCTGGC AGAAGTGAAT 
ACCCTCCTGC GGGAACACCT AGATAAAGCA AATGAGGTGA ATTTAGCCCT TAAAGAAGAT 
GTTGGAAAAC TGACAGCAGA TTGGATGAGG GCCCGAGAGG AGCTGGAATT GAAGGAGAGT 
GAATGGCGCA GTGAGCGTGA GTTTTATGAC AGCTACTTAA GGGGTGAACA TAACCGTCTT 
CTGAGCCTAT GGCGTCAGGT GCTGACTTTC CGCCGTCACT TCCTGGAAAT GAAGACTGCC 
ACTGACAGAG ATTTGTCAGA GCTGAAGGCA GAGCAAATGA GACTCTCTGG ATCTATACTT 
GTAAGCTGCT TCCGTTTGAA CTCTGGCGTA CAGCTCTGGG AGTCTGTCAC TTTGGGGAGG 
CCTGTCCTAA AGGATCAAGC ACAGCAGCAA GTGGAAAGGG AAATAAACCA GGAGGCTTTG 
GAAGTGATGT GCTTACAAGT TGATGAGGAC CTGGAGAAGA AAGAGCTTCA AGATAGGGTG 
ATGGAGCTCT CAGCCTTGCT TGTACAGTCT CAGAAACAAA ATGAAGAGAA AGAGAAGACC 
GTGAAAACAC TTAACGACAC TGTGGAGATG CTAGAAGCAA GTCGGTTAGA GGTAGAATAC 
GAAGCTTCAT TAACTAAAAA TGCCAAAGAA GAGAACCTTG CCCTTCAGAA GCTAATCAAA 
GAGATAACTG AGGTGGTGTT AGATGACAGT GACAGCACAG TCGGCATTGT CCAGCGTCCA 
GAGTCTAGCA ACGTCCTCTC TTGTCTGAGC TCCCTTGATG CAGAGCATGC CTTTGTGTTG 
GTTCAGGAAA CACTGGCAAG GAGGCAAAAA GCAATACAGG CCCTAAAAGA AGAGCTTTCT 
ACCAGGCAAG ACTCTATCAG TTTTCTGCAG CACCAGCACA GACAGCAGGA AGAGAAGTGC 
AAGAAGCTGC AGCAAAGGCT TGAGCAACTG GAGGAGGAAA ACAAGATGTC TAATAGCCAC 
CAGCAGCACC TCAAGTCTCT GGTACTAGCG CTCAAGTGTG ACTGTGAAAA CCTAGAGAAA 
ATCAGAGGAG AACTACAGCA AAAGCTGGAA TTAAGTGAGC AAGAAGCCTC ACGTCTGCGT 
CAGAGTAACA CTGAGCTGCA GCTGAAGGAA GATTCAGCCC AAGGGGAAAA GGTGGAGCAG 
CAGCTGGCAA TGGAGAGAGC ACATCATGAC CATGAGCTCG TTCTGAAGGA CTTAGCTGCA 
CTTGAAGAAA AACATTCGTT GTTACAGAAT GAATTGGTAG CCGCGAGGGA GAAGCTGGAG 
GAATCACACC TTCAGAGGGA TCTGCTGAAG CAAGAGAAAC ATGAGCTTAC AGTGGCACTG 
GAAAAGGCAG AGAAATCAGT AGCAGCATTG ACAGGGGCTC AGAATAAGTT GAATTCTGAA 
ATAGCTGACC TACATACTGC TGCAGCAAAG ATGAGCAGTA TTAATGAAGC TCTTGCACTG 
GATAAAGTGG AACTGAACAA GCTTGTGTTG CAGCTGGAAC AAGAGAATGA ACTTCTGTCA 
GATAAAGTGA ATGAGATGGA GAGAGCAAAG GTCTCTGAAC AGGAGAAGAT AACCTTGTAT 
AAAAGAACAA ATGAAGAGCT TTGTGCAGAG AAATCCCACC TGGAGAAGCT GCTGAAGAAA 
GCAGAGGAGC AACAGGAAGA GCTGCAGATG CAACTGAGAA TACTGGCAGA GGAAAAGGAA 
GAAACCCTGG AGAAACTCAA CCAGGTTTAC CGCCAGCAAG AGTCAGCTAG CAGTGGTTTG 
GAGCAGTTGC GCCAACAGTC CTCTCGCCAA GAGGATGCAG TGGCCAAGGT GTCCAAAGAG 
AAGGAATTCC TGGTGCATGA GAAGGCCGCC CTTGAGGTGC GACTGGCAGC TGTGGAACGG 
GACAGACAAG GCCTTTCAGA GCAGCTGGCA GAGGCCAGGT CAGTGAAGGA CACCCTGGAA 
TCCAGCCTGT TTGAGGCTCA GCAGCAGTTA TCTCACCTGG AGATCACCAA GAGTCAGCTT 
GAAATCCAGC TTCATACAGT CGTTCAGGCT AAGGAAGTGA TACAAGGGGA AGTGAAGTGT 
CTTCAATATG AGCTGGAAAC AGAGAGATGT CTAATGAAGC AGGAACAGAA AAACATGGCA 
CAACGGCTCA TTCAGATAGA AGAGCAGCAT AACAATACCC TGAAACTTCA GCAAACTGAT 
CATGAAGTGG AAATAAACAA ACTCCTACAA GACCTGGCAA GTGAACGGGA GGGGCGCCAG 
TTAGAGCTAC AGGAGATGCT GGAGCTCTGG GAAAAGGAGA AAGCAGAGGC AGAAGGGCAG 
CATGAGAAGA AGCTGTTTCA TATGAAGGAG AAAGTTGCTA CCATGCAAGC TCAACAAGAA 
GAGGAACGAA CCAGAGTTGA AAGTGCCAAT CAAGAGATTT TGACAGAAAA GGAGAACGAG 
AAGAAAGCTT TATTAGAGAC ACTACTCCAA ACTCAGGGAG AACTAACAGA AGCCTGCCAT 
CAGCTTGAGC AACTTAGGCA GGAGGTGAAA GAGCAGCAGG AGTATGAGCA GAACATCACA 
GAAAAACTGC AAGCAGAGCT ACAGGAAACT CACTGTAAAA TCAAGATGGT GGAGAATATG 
CACAAGGAAG AAATGGAAAA CATCAAAGAA GAAAAGAATA TCCTTCTCCA GCAGAGGGAT 
GATCTACAAA AACAGGTAGA AGAGCTGACA TCTCAGCTAG CAGCCTCCGA AGAGTCCCAT 
CAAGCAATTG GCCATAAAGC TCAGCAAGAG CTGAGTGAGG CACAAGAGTT GTCCAGGCAG 
AAGGTATTGG AGGTTGTGCA CCTTCAGAAA ATCTTGGAGG AGGAGAGGGG CCAATGGGAA 
GAGGTGGAAC ATCAGAACAA AGAGCTGCAG GTGTGCCTGC AGTCCTTAGA GGGGGAAAGG 
AGTCGATGGG AAGAAGTGGA ACGTCACAAC ACAGAATTTC AGGCTTCATT GAAGGCCCTA 
GAGAGCGAAA GAGAAAGGCT ATCTCTGTCT CTGGAACAGA AGGAATTGAG CCTCAAGACC 
TTGGAAGAAA ACAACCTTGT CCAGCAGAAC GAGGTGTCTA AACTACATTC TGCTATTCAA 
CAGGCTCAGC AGCTCCACTC AGACCACAGA AGAGAAATAC AAGAGCTCAA CAACCAGGTA 
CAGACACTAC AGGAAGTGGT GCTGGAGAAG GAAGCTAGCC TGGCAGCTCG GGAGAAGCAG 
CTATTACAGG ATCTGGAGGA GTCTCGAGCA GGCGAACGGT GCTTGAGAGA CTCTCTGCAC 
GTGCTAGAGG CTGAGATGGC TGAACTTCAT CTGAGGCTTT GTAGCACAGA GAACAGAGCA 
AAGGCATTGG CTACAGAGTG CCAGCAGGCT AATAATGCCC ACTGTGAAGC CCAGTCTCAG 
CTGGACAAAT TGCACTTGGT TCTTCACCAC GTGCTGTGTG ACAGCACTGA TAAAAGCAGA 
GACTTAGTCG CTTGGAGTTC AGAACAGGAT CATGTCTGGG GCCTAACTGT TTCTCAGGCC 
AAGGATCTCC ATGTAGAGCT CACAGTGGAC AGAGTGGCAG CAGCCCTGCA AGACCTGCGT 
CAGGACCTGA AGCAAACTCA GCAAGATCTG AATGATGCTG GGAAGAAAGT GCAGGACCTG 
GAACTGGAGC TGAGCAAGAG GCAAGCTGAG AGGGACCACT TCAGTGCCCA CAATCAGGAG 
CTACAGAAAC AGTTGGCTCA AAGCCAGGAA GAGACACAGA TGGTAGAAGG GAAGAAGAAC 
TCTCTACAAG CAGCCTTGCA GGCAGAAGCT GCTGCTCTAA AGAAGGAGGC TATTACACTA 
CATCAAGAGG TGGCATCTTT GGAAAGGAAA CTTGAGAGCG CTGAGAAGCA AAAAAAGGAT 
GTGCTGCATG AACGGGATAG ACTGCAAGCA GTTGAAGAAA AACTGATGTG GGAGATAAAA 
ATTCTTCAGG AGTCAGTCAC AGCCTCAGAA ACACGAGCAA ACACAGCAGC AGATATGAAT 
CACTCCCTTG AACAGGAACT ACAAACTACA TTGTCTGTCT TGAAAACAAA AAATAAGGAA 
GTGGATGCTC AATGGGAGAA AATCCAGATG CTACAGAAAG AGGCAGCAGA TGTAAAAGCT 
TTGCAGGAGA ATCTTATTCA AGTGAATGCC ATCCTGTCAA AGAGAGAGGG AGAAATGAAG 
TTATACCAGG AACAGATGAG AATGCTGGAA AACCAGAAAG AAATGCATAA AACTACTCTA 
GATCAAGTTA TTAAGGACAT AAAAGAGAAA AAAGAGAAGA CAGAATCCCA GCAAGAACAA 
ATACAGGAGT TAGAGAAGCA GCAAGAACTG CAAAGGACTG TTATAAGCAA AATGAGCAAA 
GACCTGGAAG ACAGAGATAA GGAGATCAGA TCCCAGCAAG AAGAAATATG GGAGCTGGAG 
AAGCAACAAG AACTGCAGAG GACTGTTGTA AGCAAGATGA CTAAAGACCT GGCACACAGA 
GACCAGGAGA TCCAATCCCA ACAAGAAGAG ATTCAGGAGC TAGAGAAGGA GCGAGAATTG 
CAGAGGACTG CTGCAAGTAA GATGAGCAAA GATCTAAAGG AGAGAGATGA GAAGATAAGA 
TCCCAGCAAG AACTGATAGA GGAGCTAGAG AAGCAGCAAG AACTGCAGAG GACTGCCTTA 
AGCAAAATGA GCAAAAACCT GGAGGAGAGA GACCAGGAGA TCAAATCCCA GCAAGAACTG 
ATAGAGGAGC TAAAGAAGCA ACAAGAACTA CAGAGGACTG CTGTGAGCAA AATGAACAAA 
GACCTGGAGG AGAGAGACCA GGAGATTAGA TCCCAGCAAG AAGAGATACA GGAACTGGAA 
AAGCAACGAG AACTGCAGAG GACTATTTTA AGCAAGATGA GCAAAGATCT GGAAGAGAAA 
GATCAGGTGA TCAAGTTCCA GGAAGGGAAG GTAATGATTC TAGAACAACA TGGTACATCA 
CAGGTGAGAA GTTTGCTAGT GGATCTTGAT CATATGAAGG GAAACTTGAA GGAGAAAAAC 
TTAGAGCTGA TGTCTCTAAA TCAGCAGATC AAAGAACTGG AGATGGAGAG AGAAGAGGTG 
AAATCTTTGC ACACAAGCCT TGAACAGCTT AGAGCAGTTC TTAGGGATAG AGAGAATGAG 
TCTGACTCTC AAAGGGATCA GTTAAGACTC TTTCAGCAGT ACAAGGAGCA TCAAGAGGAG 
TACCTGCAAG AGCTCCAGGA TAAAGTAGAA AAAATGACAC TTTCCTTATC TAAAAAAGAT 
CAAGAGCTCG AGTCACAGCA AAAGCAAATC CAGGAAGCTG AAGAAGTCAT GGAAAAGAAG 
TTGAAGACTG TTTGTGACCA ACTGGAGCAG ACATTAGAAA CCTTAAAAGA AAAGGAAAGA 
CTCCTAGACA TCCAAAAGCA ACAAACAAGG GAATATGAGG AAAAAACAGA ACAGATGAAT 
GTCTTATGTA GAGACTTAGA ATACACTAAG GCAATATTGA GAGAGAAGGA TTTAATGATT 
GAATCTCAGA AGGAACTGAT TGAGACCTTC CAAAAACAAG AAGACGACTC TATGCAGCAG 
AAAGAAATTC TGCAGCATCT TAAAGGGGCT TTAAAGGAAC AAGAACAAGA AACTTTATCT 
CTTAGAAAGC AGTGTGAGGC ATTTAAAGAA AAGGAGGAAA AGCACAAAAC TGATCAAGTG 
ATTCTTCAAG AAACAAAAAT CACTCTGAAA GAAAGAGAAA GAAAGATAGA GGTTTTAGAG 
AAAGCTGTCT CTAAGCTTCA ACAACAAAAT GATGATACGA TGATGCAGAC TACAGCTATA 
GTGCAAAAAC TACAATGTGC TGAATCTTCC TTAGCAGCTA GGGATCAAGA GATAGCATCT 
CTGAAAGAGC ATGTCCAGGA ACTTCAGAAG CAGAAGGAGT CGGAAGCTAA ACAGGTCAAA 
AGTCTACAGC AGGATCTAAA CAAAATGAGC AAAACAATGA AGAAGAACAA TTTGCAGATT 
CTCAAACAGT CGGAGCAGAT TAATGTGTTT CATATTTGTG AAGAAAGTAG GAAAGTAGCA 
CTAACATCTT GCCAGAATCA AGTGAGTCTG CTTGAGGAAG TGGTGAGAAA GAGAGATGAA 
GACAATGAAA CTCTTCTGCA AAAACTCCAG CACCAAGAAG AAGAACTGAA GACCTTGCAG 
AATCTCCAGC TTATGCTATC TGAGAAGAAA GAGGAGATTA AACGTCACGG AGAGGAAGAG 
AAGTTCCCAG AAGAAGCCTT GCATGGGAGT GAATGGGAGA CGAAGGCTCA AGGTGAGCAA 
AAAAAGTCAG AAGATGAGGA AATAAGAGGT CTTCATGAAG ATCTCCAGTA TGTTCAGCAG 
ACTCTGACAA AGAAGGATGA AGAGATCAAA TACCAGAGAG ACAGAGTCAA GTATTTAGAG 
AAAACTCTGG CAGGGAAAGA AAAAGAGCTG AGGAGGCAGA GTGAACTCTT GAAACAATTA 
ACATCAGCTC TGCATTGGAA AGATGAAGGG GAGACTCTAA AGAAACAAAT CCAGAAACTC 
CAAAAATGGG AGGAAGAAGA AGCAGAGAAC AGGAGGGTTC TCCAAGAGAG AGACCAATTC 
TTGCAAAGGC AGAAGGAACT AGCCCAACAA CTGGAAGATG AGAGGAAAGT AAAGGGGGAA 
GAACTGAAGC GTGTGATTGC TATTTTGAAG CAGACTGAAA GTGGCGAAAT CGAATGGAAA 
GAGAAGGCAC AAGCACTGAC TCTTGCCCTT TCTAAGAGTG AAATGACCAA TGGGACTTTG 
AGGGAAGAAA TAGCCATTCT GCAGAGTATG GTTTCAGAGA GGGACAAGGA CCGGTTTCAT 
CTTCAGGAGG CTTTTGAAGG CGGTGAGAAC CTATCGTGGC TCTTGGAAAA GAAGCTCCTA 
TGGCAGCGGC TGGAGTGTCT GCAGCAAGCA GTTGCAAGGC TGGAACATGA AAAGTCTGAG 
CTGAAGCAAC TCAATGTTGA GCTCAAGATA ACTCTTGAGC AGGTGGAACG TGAACGGAGG 
AGGCTGAAGA GAAGTTGTAG TCATCAGTCA CAGCCAAATG CAAACAGGTT TTCTCTCTCG 
GAATCTGATG AGGACAAGAT GCCTGCTTCT AGAAAGGAGG AGGCTCGTGC TCTCTGCAGT 
CAATTAGCTG AGTTACAGAA CCAGGTGACT CTTCTGCAGC AGCAGTTGGC ACAAGAACGA 
AAATACAAGC AGGACTATAT TGAGTGTTGT GCAAAAACCA GTCAGGAGCT TTTGGACCTT 
CACCAAGAGC TGTCTTACTC CCTGGAGGCT GTGATCAGGG AGCCCAAAGC TGCTGTTCTG 
GAGGCAGAGA CTCAGAAGCT GGATCTATCT CTGAACCATA CCTTGGCACT AATATCTTTG 
GACTGCCGAT CTCCTGAGAG ACAGCTGTTG CACTCAGCAG TGAGATCCAC CACAAGGGAT 
GGTCTGAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947002.1
CDS	186..7757
Translation	MLLQYRSWCRELEQRLEAGGVSVQGSLSYRWETTEDHSLEKALLQLEEEQQRCENLAEVNTLLREHLDKANEVNLALKEDVGKLTADWMRAREELELKESEWRSEREFYDSYLRGEHNRLLSLWRQVLTFRRHFLEMKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVTLLQQQLAQERKYKQDYIECCAKTSQELLDLHQELSYSLEAVIREPKAAVLEAETQKLDLSLNHTLALISLDCRSPERQLLHSAVRSTTRDGLR

Target ORF information:

RefSeq Version	XM_004946945.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946945.2

ORF Insert Sequence:

ATGCTGCTGC AGTATCGGAG CTGGTGTCGT GAGCTGGAGC AGCGACTTGA AGCAGGAGGG 
GTGAGTGTGC AGGGATCCCT TTCATACAGA TGGGAAACCA CTGAAGACCA CAGCCTGGAG 
AAGGCACTCC TCCAGTTAGA AGAAGAGCAG CAAAGGTGTG AGAATCTGGC AGAAGTGAAT 
ACCCTCCTGC GGGAACACCT AGATAAAGCA AATGAGGTGA ATTTAGCCCT TAAAGAAGAT 
GTTGGAAAAC TGACAGCAGA TTGGATGAGG GCCCGAGAGG AGCTGGAATT GAAGGAGAGT 
GAATGGCGCA GTGAGCGTGA GTTTTATGAC AGCTACTTAA GGGGTGAACA TAACCGTCTT 
CTGAGCCTAT GGCGTCAGGT GCTGACTTTC CGCCGTCACT TCCTGGAAAT GAAGACTGCC 
ACTGACAGAG ATTTGTCAGA GCTGAAGGCA GAGCAAATGA GACTCTCTGG ATCTATACTT 
GTAAGCTGCT TCCGTTTGAA CTCTGGCGTA CAGCTCTGGG AGTCTGTCAC TTTGGGGAGG 
CCTGTCCTAA AGGATCAAGC ACAGCAGCAA GTGGAAAGGG AAATAAACCA GGAGGCTTTG 
GAAGTGATGT GCTTACAAGT TGATGAGGAC CTGGAGAAGA AAGAGCTTCA AGATAGGGTG 
ATGGAGCTCT CAGCCTTGCT TGTACAGTCT CAGAAACAAA ATGAAGAGAA AGAGAAGACC 
GTGAAAACAC TTAACGACAC TGTGGAGATG CTAGAAGCAA GTCGGTTAGA GGTAGAATAC 
GAAGCTTCAT TAACTAAAAA TGCCAAAGAA GAGAACCTTG CCCTTCAGAA GCTAATCAAA 
GAGATAACTG AGGTGGTGTT AGATGACAGT GACAGCACAG TCGGCATTGT CCAGCGTCCA 
GAGTCTAGCA ACGTCCTCTC TTGTCTGAGC TCCCTTGATG CAGAGCATGC CTTTGTGTTG 
GTTCAGGAAA CACTGGCAAG GAGGCAAAAA GCAATACAGG CCCTAAAAGA AGAGCTTTCT 
ACCAGGCAAG ACTCTATCAG TTTTCTGCAG CACCAGCACA GACAGCAGGA AGAGAAGTGC 
AAGAAGCTGC AGCAAAGGCT TGAGCAACTG GAGGAGGAAA ACAAGATGTC TAATAGCCAC 
CAGCAGCACC TCAAGTCTCT GGTACTAGCG CTCAAGTGTG ACTGTGAAAA CCTAGAGAAA 
ATCAGAGGAG AACTACAGCA AAAGCTGGAA TTAAGTGAGC AAGAAGCCTC ACGTCTGCGT 
CAGAGTAACA CTGAGCTGCA GCTGAAGGAA GATTCAGCCC AAGGGGAAAA GGTGGAGCAG 
CAGCTGGCAA TGGAGAGAGC ACATCATGAC CATGAGCTCG TTCTGAAGGA CTTAGCTGCA 
CTTGAAGAAA AACATTCGTT GTTACAGAAT GAATTGGTAG CCGCGAGGGA GAAGCTGGAG 
GAATCACACC TTCAGAGGGA TCTGCTGAAG CAAGAGAAAC ATGAGCTTAC AGTGGCACTG 
GAAAAGGCAG AGAAATCAGT AGCAGCATTG ACAGGGGCTC AGAATAAGTT GAATTCTGAA 
ATAGCTGACC TACATACTGC TGCAGCAAAG ATGAGCAGTA TTAATGAAGC TCTTGCACTG 
GATAAAGTGG AACTGAACAA GCTTGTGTTG CAGCTGGAAC AAGAGAATGA ACTTCTGTCA 
GATAAAGTGA ATGAGATGGA GAGAGCAAAG GTCTCTGAAC AGGAGAAGAT AACCTTGTAT 
AAAAGAACAA ATGAAGAGCT TTGTGCAGAG AAATCCCACC TGGAGAAGCT GCTGAAGAAA 
GCAGAGGAGC AACAGGAAGA GCTGCAGATG CAACTGAGAA TACTGGCAGA GGAAAAGGAA 
GAAACCCTGG AGAAACTCAA CCAGGTTTAC CGCCAGCAAG AGTCAGCTAG CAGTGGTTTG 
GAGCAGTTGC GCCAACAGTC CTCTCGCCAA GAGGATGCAG TGGCCAAGGT GTCCAAAGAG 
AAGGAATTCC TGGTGCATGA GAAGGCCGCC CTTGAGGTGC GACTGGCAGC TGTGGAACGG 
GACAGACAAG GCCTTTCAGA GCAGCTGGCA GAGGCCAGGT CAGTGAAGGA CACCCTGGAA 
TCCAGCCTGT TTGAGGCTCA GCAGCAGTTA TCTCACCTGG AGATCACCAA GAGTCAGCTT 
GAAATCCAGC TTCATACAGT CGTTCAGGCT AAGGAAGTGA TACAAGGGGA AGTGAAGTGT 
CTTCAATATG AGCTGGAAAC AGAGAGATGT CTAATGAAGC AGGAACAGAA AAACATGGCA 
CAACGGCTCA TTCAGATAGA AGAGCAGCAT AACAATACCC TGAAACTTCA GCAAACTGAT 
CATGAAGTGG AAATAAACAA ACTCCTACAA GACCTGGCAA GTGAACGGGA GGGGCGCCAG 
TTAGAGCTAC AGGAGATGCT GGAGCTCTGG GAAAAGGAGA AAGCAGAGGC AGAAGGGCAG 
CATGAGAAGA AGCTGTTTCA TATGAAGGAG AAAGTTGCTA CCATGCAAGC TCAACAAGAA 
GAGGAACGAA CCAGAGTTGA AAGTGCCAAT CAAGAGATTT TGACAGAAAA GGAGAACGAG 
AAGAAAGCTT TATTAGAGAC ACTACTCCAA ACTCAGGGAG AACTAACAGA AGCCTGCCAT 
CAGCTTGAGC AACTTAGGCA GGAGGTGAAA GAGCAGCAGG AGTATGAGCA GAACATCACA 
GAAAAACTGC AAGCAGAGCT ACAGGAAACT CACTGTAAAA TCAAGATGGT GGAGAATATG 
CACAAGGAAG AAATGGAAAA CATCAAAGAA GAAAAGAATA TCCTTCTCCA GCAGAGGGAT 
GATCTACAAA AACAGGTAGA AGAGCTGACA TCTCAGCTAG CAGCCTCCGA AGAGTCCCAT 
CAAGCAATTG GCCATAAAGC TCAGCAAGAG CTGAGTGAGG CACAAGAGTT GTCCAGGCAG 
AAGGTATTGG AGGTTGTGCA CCTTCAGAAA ATCTTGGAGG AGGAGAGGGG CCAATGGGAA 
GAGGTGGAAC ATCAGAACAA AGAGCTGCAG GTGTGCCTGC AGTCCTTAGA GGGGGAAAGG 
AGTCGATGGG AAGAAGTGGA ACGTCACAAC ACAGAATTTC AGGCTTCATT GAAGGCCCTA 
GAGAGCGAAA GAGAAAGGCT ATCTCTGTCT CTGGAACAGA AGGAATTGAG CCTCAAGACC 
TTGGAAGAAA ACAACCTTGT CCAGCAGAAC GAGGTGTCTA AACTACATTC TGCTATTCAA 
CAGGCTCAGC AGCTCCACTC AGACCACAGA AGAGAAATAC AAGAGCTCAA CAACCAGGTA 
CAGACACTAC AGGAAGTGGT GCTGGAGAAG GAAGCTAGCC TGGCAGCTCG GGAGAAGCAG 
CTATTACAGG ATCTGGAGGA GTCTCGAGCA GGCGAACGGT GCTTGAGAGA CTCTCTGCAC 
GTGCTAGAGG CTGAGATGGC TGAACTTCAT CTGAGGCTTT GTAGCACAGA GAACAGAGCA 
AAGGCATTGG CTACAGAGTG CCAGCAGGCT AATAATGCCC ACTGTGAAGC CCAGTCTCAG 
CTGGACAAAT TGCACTTGGT TCTTCACCAC GTGCTGTGTG ACAGCACTGA TAAAAGCAGA 
GACTTAGTCG CTTGGAGTTC AGAACAGGAT CATGTCTGGG GCCTAACTGT TTCTCAGGCC 
AAGGATCTCC ATGTAGAGCT CACAGTGGAC AGAGTGGCAG CAGCCCTGCA AGACCTGCGT 
CAGGACCTGA AGCAAACTCA GCAAGATCTG AATGATGCTG GGAAGAAAGT GCAGGACCTG 
GAACTGGAGC TGAGCAAGAG GCAAGCTGAG AGGGACCACT TCAGTGCCCA CAATCAGGAG 
CTACAGAAAC AGTTGGCTCA AAGCCAGGAA GAGACACAGA TGGTAGAAGG GAAGAAGAAC 
TCTCTACAAG CAGCCTTGCA GGCAGAAGCT GCTGCTCTAA AGAAGGAGGC TATTACACTA 
CATCAAGAGG TGGCATCTTT GGAAAGGAAA CTTGAGAGCG CTGAGAAGCA AAAAAAGGAT 
GTGCTGCATG AACGGGATAG ACTGCAAGCA GTTGAAGAAA AACTGATGTG GGAGATAAAA 
ATTCTTCAGG AGTCAGTCAC AGCCTCAGAA ACACGAGCAA ACACAGCAGC AGATATGAAT 
CACTCCCTTG AACAGGAACT ACAAACTACA TTGTCTGTCT TGAAAACAAA AAATAAGGAA 
GTGGATGCTC AATGGGAGAA AATCCAGATG CTACAGAAAG AGGCAGCAGA TGTAAAAGCT 
TTGCAGGAGA ATCTTATTCA AGTGAATGCC ATCCTGTCAA AGAGAGAGGG AGAAATGAAG 
TTATACCAGG AACAGATGAG AATGCTGGAA AACCAGAAAG AAATGCATAA AACTACTCTA 
GATCAAGTTA TTAAGGACAT AAAAGAGAAA AAAGAGAAGA CAGAATCCCA GCAAGAACAA 
ATACAGGAGT TAGAGAAGCA GCAAGAACTG CAAAGGACTG TTATAAGCAA AATGAGCAAA 
GACCTGGAAG ACAGAGATAA GGAGATCAGA TCCCAGCAAG AAGAAATATG GGAGCTGGAG 
AAGCAACAAG AACTGCAGAG GACTGTTGTA AGCAAGATGA CTAAAGACCT GGCACACAGA 
GACCAGGAGA TCCAATCCCA ACAAGAAGAG ATTCAGGAGC TAGAGAAGGA GCGAGAATTG 
CAGAGGACTG CTGCAAGTAA GATGAGCAAA GATCTAAAGG AGAGAGATGA GAAGATAAGA 
TCCCAGCAAG AACTGATAGA GGAGCTAGAG AAGCAGCAAG AACTGCAGAG GACTGCCTTA 
AGCAAAATGA GCAAAAACCT GGAGGAGAGA GACCAGGAGA TCAAATCCCA GCAAGAACTG 
ATAGAGGAGC TAAAGAAGCA ACAAGAACTA CAGAGGACTG CTGTGAGCAA AATGAACAAA 
GACCTGGAGG AGAGAGACCA GGAGATTAGA TCCCAGCAAG AAGAGATACA GGAACTGGAA 
AAGCAACGAG AACTGCAGAG GACTATTTTA AGCAAGATGA GCAAAGATCT GGAAGAGAAA 
GATCAGGTGA TCAAGTTCCA GGAAGGGAAG GTAATGATTC TAGAACAACA TGGTACATCA 
CAGGTGAGAA GTTTGCTAGT GGATCTTGAT CATATGAAGG GAAACTTGAA GGAGAAAAAC 
TTAGAGCTGA TGTCTCTAAA TCAGCAGATC AAAGAACTGG AGATGGAGAG AGAAGAGGTG 
AAATCTTTGC ACACAAGCCT TGAACAGCTT AGAGCAGTTC TTAGGGATAG AGAGAATGAG 
TCTGACTCTC AAAGGGATCA GTTAAGACTC TTTCAGCAGT ACAAGGAGCA TCAAGAGGAG 
TACCTGCAAG AGCTCCAGGA TAAAGTAGAA AAAATGACAC TTTCCTTATC TAAAAAAGAT 
CAAGAGCTCG AGTCACAGCA AAAGCAAATC CAGGAAGCTG AAGAAGTCAT GGAAAAGAAG 
TTGAAGACTG TTTGTGACCA ACTGGAGCAG ACATTAGAAA CCTTAAAAGA AAAGGAAAGA 
CTCCTAGACA TCCAAAAGCA ACAAACAAGG GAATATGAGG AAAAAACAGA ACAGATGAAT 
GTCTTATGTA GAGACTTAGA ATACACTAAG GCAATATTGA GAGAGAAGGA TTTAATGATT 
GAATCTCAGA AGGAACTGAT TGAGACCTTC CAAAAACAAG AAGACGACTC TATGCAGCAG 
AAAGAAATTC TGCAGCATCT TAAAGGGGCT TTAAAGGAAC AAGAACAAGA AACTTTATCT 
CTTAGAAAGC AGTGTGAGGC ATTTAAAGAA AAGGAGGAAA AGCACAAAAC TGATCAAGTG 
ATTCTTCAAG AAACAAAAAT CACTCTGAAA GAAAGAGAAA GAAAGATAGA GGTTTTAGAG 
AAAGCTGTCT CTAAGCTTCA ACAACAAAAT GATGATACGA TGATGCAGAC TACAGCTATA 
GTGCAAAAAC TACAATGTGC TGAATCTTCC TTAGCAGCTA GGGATCAAGA GATAGCATCT 
CTGAAAGAGC ATGTCCAGGA ACTTCAGAAG CAGAAGGAGT CGGAAGCTAA ACAGGTCAAA 
AGTCTACAGC AGGATCTAAA CAAAATGAGC AAAACAATGA AGAAGAACAA TTTGCAGATT 
CTCAAACAGT CGGAGCAGAT TAATGTGTTT CATATTTGTG AAGAAAGTAG GAAAGTAGCA 
CTAACATCTT GCCAGAATCA AGTGAGTCTG CTTGAGGAAG TGGTGAGAAA GAGAGATGAA 
GACAATGAAA CTCTTCTGCA AAAACTCCAG CACCAAGAAG AAGAACTGAA GACCTTGCAG 
AATCTCCAGC TTATGCTATC TGAGAAGAAA GAGGAGATTA AACGTCACGG AGAGGAAGAG 
AAGTTCCCAG AAGAAGCCTT GCATGGGAGT GAATGGGAGA CGAAGGCTCA AGGTGAGCAA 
AAAAAGTCAG AAGATGAGGA AATAAGAGGT CTTCATGAAG ATCTCCAGTA TGTTCAGCAG 
ACTCTGACAA AGAAGGATGA AGAGATCAAA TACCAGAGAG ACAGAGTCAA GTATTTAGAG 
AAAACTCTGG CAGGGAAAGA AAAAGAGCTG AGGAGGCAGA GTGAACTCTT GAAACAATTA 
ACATCAGCTC TGCATTGGAA AGATGAAGGG GAGACTCTAA AGAAACAAAT CCAGAAACTC 
CAAAAATGGG AGGAAGAAGA AGCAGAGAAC AGGAGGGTTC TCCAAGAGAG AGACCAATTC 
TTGCAAAGGC AGAAGGAACT AGCCCAACAA CTGGAAGATG AGAGGAAAGT AAAGGGGGAA 
GAACTGAAGC GTGTGATTGC TATTTTGAAG CAGACTGAAA GTGGCGAAAT CGAATGGAAA 
GAGAAGGCAC AAGCACTGAC TCTTGCCCTT TCTAAGAGTG AAATGACCAA TGGGACTTTG 
AGGGAAGAAA TAGCCATTCT GCAGAGTATG GTTTCAGAGA GGGACAAGGA CCGGTTTCAT 
CTTCAGGAGG CTTTTGAAGG CGGTGAGAAC CTATCGTGGC TCTTGGAAAA GAAGCTCCTA 
TGGCAGCGGC TGGAGTGTCT GCAGCAAGCA GTTGCAAGGC TGGAACATGA AAAGTCTGAG 
CTGAAGCAAC TCAATGTTGA GCTCAAGATA ACTCTTGAGC AGGTGGAACG TGAACGGAGG 
AGGCTGAAGA GAAGTTGTAG TCATCAGTCA CAGCCAAATG CAAACAGGTT TTCTCTCTCG 
GAATCTGATG AGGACAAGAT GCCTGCTTCT AGAAAGGAGG AGGCTCGTGC TCTCTGCAGT 
CAATTAGCTG AGTTACAGAA CCAGGTGACT CTTCTGCAGC AGCAGTTGGC ACAAGAACGA 
AAATACAAGC AGGACTATAT TGAGTGTTGT GCAAAAACCA GTCAGGAGCT TTTGGACCTT 
CACCAAGAGC TGTCTTACTC CCTGGAGGCT GTGATCAGGG AGCCCAAAGC TGCTGTTCTG 
GAGGCAGAGA CTCAGAAGCT GGATCTATCT CTGAACCATA CCTTGGCACT AATATCTTTG 
GACTGCCGAT CTCCTGAGAG ACAGCTGTTG CACTCAGCAG TGAGATCCAC CACAAGGGAT 
GGTCTGAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_004946947.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947004.1 Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

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CloneID	OGa17584
Clone ID Related Accession (Same CDS sequence)	XM_004946947.2 , XM_004946947.3
Accession Version	XM_004946947.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455990.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946947.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGAGGAA TAGAAAAGCT GTCATCAAAA GCTGCTGTAA CTGCACTGCT 
GAACACAACT TTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947004.1
CDS	14..7471
Translation	MAAREAARGQACLRRRLHGAEEAQQQQAVLVRKLQEKLLQYRSWCRELEQRLEAGGVSVQGSLSYRWETTEDHSLEKALLQLEEEQQRCENLAEVNTLLREHLDKANEVNLALKEDVGKLTADWMRAREELELKESEWRSEREFYDSYLRGEHNRLLSLWRQVLTFRRHFLEMKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVRNRKAVIKSCCNCTAEHNFA

Target ORF information:

RefSeq Version	XM_004946947.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946947.2

ORF Insert Sequence:

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGAGGAA TAGAAAAGCT GTCATCAAAA GCTGCTGTAA CTGCACTGCT 
GAACACAACT TTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_004946947.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947004.1 Gallus gallus centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

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CloneID	OGa17584
Clone ID Related Accession (Same CDS sequence)	XM_004946947.2 , XM_004946947.3
Accession Version	XM_004946947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004946947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGAGGAA TAGAAAAGCT GTCATCAAAA GCTGCTGTAA CTGCACTGCT 
GAACACAACT TTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947004.1
CDS	16..7473
Translation	MAAREAARGQACLRRRLHGAEEAQQQQAVLVRKLQEKLLQYRSWCRELEQRLEAGGVSVQGSLSYRWETTEDHSLEKALLQLEEEQQRCENLAEVNTLLREHLDKANEVNLALKEDVGKLTADWMRAREELELKESEWRSEREFYDSYLRGEHNRLLSLWRQVLTFRRHFLEMKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVRNRKAVIKSCCNCTAEHNFA

Target ORF information:

RefSeq Version	XM_004946947.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946947.3

ORF Insert Sequence:

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGAGGAA TAGAAAAGCT GTCATCAAAA GCTGCTGTAA CTGCACTGCT 
GAACACAACT TTGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_004946950.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004947007.1 Gallus gallus centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

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CloneID	OGa17587
Clone ID Related Accession (Same CDS sequence)	XM_004946950.2
Accession Version	XM_004946950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004946950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTG CCACTGACAG AGATTTGTCA GAGCTGAAGG CAGAGCAAAT GAGACTCTCT 
GGATCTATAC TTGTAAGCTG CTTCCGTTTG AACTCTGGCG TACAGCTCTG GGAGTCTGTC 
ACTTTGGGGA GGCCTGTCCT AAAGGATCAA GCACAGCAGC AAGTGGAAAG GGAAATAAAC 
CAGGAGGCTT TGGAAGTGAT GTGCTTACAA GTTGATGAGG ACCTGGAGAA GAAAGAGCTT 
CAAGATAGGG TGATGGAGCT CTCAGCCTTG CTTGTACAGT CTCAGAAACA AAATGAAGAG 
AAAGAGAAGA CCGTGAAAAC ACTTAACGAC ACTGTGGAGA TGCTAGAAGC AAGTCGGTTA 
GAGGTAGAAT ACGAAGCTTC ATTAACTAAA AATGCCAAAG AAGAGAACCT TGCCCTTCAG 
AAGCTAATCA AAGAGATAAC TGAGGTGGTG TTAGATGACA GTGACAGCAC AGTCGGCATT 
GTCCAGCGTC CAGAGTCTAG CAACGTCCTC TCTTGTCTGA GCTCCCTTGA TGCAGAGCAT 
GCCTTTGTGT TGGTTCAGGA AACACTGGCA AGGAGGCAAA AAGCAATACA GGCCCTAAAA 
GAAGAGCTTT CTACCAGGCA AGACTCTATC AGTTTTCTGC AGCACCAGCA CAGACAGCAG 
GAAGAGAAGT GCAAGAAGCT GCAGCAAAGG CTTGAGCAAC TGGAGGAGGA AAACAAGATG 
TCTAATAGCC ACCAGCAGCA CCTCAAGTCT CTGGTACTAG CGCTCAAGTG TGACTGTGAA 
AACCTAGAGA AAATCAGAGG AGAACTACAG CAAAAGCTGG AATTAAGTGA GCAAGAAGCC 
TCACGTCTGC GTCAGAGTAA CACTGAGCTG CAGCTGAAGG AAGATTCAGC CCAAGGGGAA 
AAGGTGGAGC AGCAGCTGGC AATGGAGAGA GCACATCATG ACCATGAGCT CGTTCTGAAG 
GACTTAGCTG CACTTGAAGA AAAACATTCG TTGTTACAGA ATGAATTGGT AGCCGCGAGG 
GAGAAGCTGG AGGAATCACA CCTTCAGAGG GATCTGCTGA AGCAAGAGAA ACATGAGCTT 
ACAGTGGCAC TGGAAAAGGC AGAGAAATCA GTAGCAGCAT TGACAGGGGC TCAGAATAAG 
TTGAATTCTG AAATAGCTGA CCTACATACT GCTGCAGCAA AGATGAGCAG TATTAATGAA 
GCTCTTGCAC TGGATAAAGT GGAACTGAAC AAGCTTGTGT TGCAGCTGGA ACAAGAGAAT 
GAACTTCTGT CAGATAAAGT GAATGAGATG GAGAGAGCAA AGGTCTCTGA ACAGGAGAAG 
ATAACCTTGT ATAAAAGAAC AAATGAAGAG CTTTGTGCAG AGAAATCCCA CCTGGAGAAG 
CTGCTGAAGA AAGCAGAGGA GCAACAGGAA GAGCTGCAGA TGCAACTGAG AATACTGGCA 
GAGGAAAAGG AAGAAACCCT GGAGAAACTC AACCAGGTTT ACCGCCAGCA AGAGTCAGCT 
AGCAGTGGTT TGGAGCAGTT GCGCCAACAG TCCTCTCGCC AAGAGGATGC AGTGGCCAAG 
GTGTCCAAAG AGAAGGAATT CCTGGTGCAT GAGAAGGCCG CCCTTGAGGT GCGACTGGCA 
GCTGTGGAAC GGGACAGACA AGGCCTTTCA GAGCAGCTGG CAGAGGCCAG GTCAGTGAAG 
GACACCCTGG AATCCAGCCT GTTTGAGGCT CAGCAGCAGT TATCTCACCT GGAGATCACC 
AAGAGTCAGC TTGAAATCCA GCTTCATACA GTCGTTCAGG CTAAGGAAGT GATACAAGGG 
GAAGTGAAGT GTCTTCAATA TGAGCTGGAA ACAGAGAGAT GTCTAATGAA GCAGGAACAG 
AAAAACATGG CACAACGGCT CATTCAGATA GAAGAGCAGC ATAACAATAC CCTGAAACTT 
CAGCAAACTG ATCATGAAGT GGAAATAAAC AAACTCCTAC AAGACCTGGC AAGTGAACGG 
GAGGGGCGCC AGTTAGAGCT ACAGGAGATG CTGGAGCTCT GGGAAAAGGA GAAAGCAGAG 
GCAGAAGGGC AGCATGAGAA GAAGCTGTTT CATATGAAGG AGAAAGTTGC TACCATGCAA 
GCTCAACAAG AAGAGGAACG AACCAGAGTT GAAAGTGCCA ATCAAGAGAT TTTGACAGAA 
AAGGAGAACG AGAAGAAAGC TTTATTAGAG ACACTACTCC AAACTCAGGG AGAACTAACA 
GAAGCCTGCC ATCAGCTTGA GCAACTTAGG CAGGAGGTGA AAGAGCAGCA GGAGTATGAG 
CAGAACATCA CAGAAAAACT GCAAGCAGAG CTACAGGAAA CTCACTGTAA AATCAAGATG 
GTGGAGAATA TGCACAAGGA AGAAATGGAA AACATCAAAG AAGAAAAGAA TATCCTTCTC 
CAGCAGAGGG ATGATCTACA AAAACAGGTA GAAGAGCTGA CATCTCAGCT AGCAGCCTCC 
GAAGAGTCCC ATCAAGCAAT TGGCCATAAA GCTCAGCAAG AGCTGAGTGA GGCACAAGAG 
TTGTCCAGGC AGAAGGTATT GGAGGTTGTG CACCTTCAGA AAATCTTGGA GGAGGAGAGG 
GGCCAATGGG AAGAGGTGGA ACATCAGAAC AAAGAGCTGC AGGTGTGCCT GCAGTCCTTA 
GAGGGGGAAA GGAGTCGATG GGAAGAAGTG GAACGTCACA ACACAGAATT TCAGGCTTCA 
TTGAAGGCCC TAGAGAGCGA AAGAGAAAGG CTATCTCTGT CTCTGGAACA GAAGGAATTG 
AGCCTCAAGA CCTTGGAAGA AAACAACCTT GTCCAGCAGA ACGAGGTGTC TAAACTACAT 
TCTGCTATTC AACAGGCTCA GCAGCTCCAC TCAGACCACA GAAGAGAAAT ACAAGAGCTC 
AACAACCAGG TACAGACACT ACAGGAAGTG GTGCTGGAGA AGGAAGCTAG CCTGGCAGCT 
CGGGAGAAGC AGCTATTACA GGATCTGGAG GAGTCTCGAG CAGGCGAACG GTGCTTGAGA 
GACTCTCTGC ACGTGCTAGA GGCTGAGATG GCTGAACTTC ATCTGAGGCT TTGTAGCACA 
GAGAACAGAG CAAAGGCATT GGCTACAGAG TGCCAGCAGG CTAATAATGC CCACTGTGAA 
GCCCAGTCTC AGCTGGACAA ATTGCACTTG GTTCTTCACC ACGTGCTGTG TGACAGCACT 
GATAAAAGCA GAGACTTAGT CGCTTGGAGT TCAGAACAGG ATCATGTCTG GGGCCTAACT 
GTTTCTCAGG CCAAGGATCT CCATGTAGAG CTCACAGTGG ACAGAGTGGC AGCAGCCCTG 
CAAGACCTGC GTCAGGACCT GAAGCAAACT CAGCAAGATC TGAATGATGC TGGGAAGAAA 
GTGCAGGACC TGGAACTGGA GCTGAGCAAG AGGCAAGCTG AGAGGGACCA CTTCAGTGCC 
CACAATCAGG AGCTACAGAA ACAGTTGGCT CAAAGCCAGG AAGAGACACA GATGGTAGAA 
GGGAAGAAGA ACTCTCTACA AGCAGCCTTG CAGGCAGAAG CTGCTGCTCT AAAGAAGGAG 
GCTATTACAC TACATCAAGA GGTGGCATCT TTGGAAAGGA AACTTGAGAG CGCTGAGAAG 
CAAAAAAAGG ATGTGCTGCA TGAACGGGAT AGACTGCAAG CAGTTGAAGA AAAACTGATG 
TGGGAGATAA AAATTCTTCA GGAGTCAGTC ACAGCCTCAG AAACACGAGC AAACACAGCA 
GCAGATATGA ATCACTCCCT TGAACAGGAA CTACAAACTA CATTGTCTGT CTTGAAAACA 
AAAAATAAGG AAGTGGATGC TCAATGGGAG AAAATCCAGA TGCTACAGAA AGAGGCAGCA 
GATGTAAAAG CTTTGCAGGA GAATCTTATT CAAGTGAATG CCATCCTGTC AAAGAGAGAG 
GGAGAAATGA AGTTATACCA GGAACAGATG AGAATGCTGG AAAACCAGAA AGAAATGCAT 
AAAACTACTC TAGATCAAGT TATTAAGGAC ATAAAAGAGA AAAAAGAGAA GACAGAATCC 
CAGCAAGAAC AAATACAGGA GTTAGAGAAG CAGCAAGAAC TGCAAAGGAC TGTTATAAGC 
AAAATGAGCA AAGACCTGGA AGACAGAGAT AAGGAGATCA GATCCCAGCA AGAAGAAATA 
TGGGAGCTGG AGAAGCAACA AGAACTGCAG AGGACTGTTG TAAGCAAGAT GACTAAAGAC 
CTGGCACACA GAGACCAGGA GATCCAATCC CAACAAGAAG AGATTCAGGA GCTAGAGAAG 
GAGCGAGAAT TGCAGAGGAC TGCTGCAAGT AAGATGAGCA AAGATCTAAA GGAGAGAGAT 
GAGAAGATAA GATCCCAGCA AGAACTGATA GAGGAGCTAG AGAAGCAGCA AGAACTGCAG 
AGGACTGCCT TAAGCAAAAT GAGCAAAAAC CTGGAGGAGA GAGACCAGGA GATCAAATCC 
CAGCAAGAAC TGATAGAGGA GCTAAAGAAG CAACAAGAAC TACAGAGGAC TGCTGTGAGC 
AAAATGAACA AAGACCTGGA GGAGAGAGAC CAGGAGATTA GATCCCAGCA AGAAGAGATA 
CAGGAACTGG AAAAGCAACG AGAACTGCAG AGGACTATTT TAAGCAAGAT GAGCAAAGAT 
CTGGAAGAGA AAGATCAGGT GATCAAGTTC CAGGAAGGGA AGGTAATGAT TCTAGAACAA 
CATGGTACAT CACAGGTGAG AAGTTTGCTA GTGGATCTTG ATCATATGAA GGGAAACTTG 
AAGGAGAAAA ACTTAGAGCT GATGTCTCTA AATCAGCAGA TCAAAGAACT GGAGATGGAG 
AGAGAAGAGG TGAAATCTTT GCACACAAGC CTTGAACAGC TTAGAGCAGT TCTTAGGGAT 
AGAGAGAATG AGTCTGACTC TCAAAGGGAT CAGTTAAGAC TCTTTCAGCA GTACAAGGAG 
CATCAAGAGG AGTACCTGCA AGAGCTCCAG GATAAAGTAG AAAAAATGAC ACTTTCCTTA 
TCTAAAAAAG ATCAAGAGCT CGAGTCACAG CAAAAGCAAA TCCAGGAAGC TGAAGAAGTC 
ATGGAAAAGA AGTTGAAGAC TGTTTGTGAC CAACTGGAGC AGACATTAGA AACCTTAAAA 
GAAAAGGAAA GACTCCTAGA CATCCAAAAG CAACAAACAA GGGAATATGA GGAAAAAACA 
GAACAGATGA ATGTCTTATG TAGAGACTTA GAATACACTA AGGCAATATT GAGAGAGAAG 
GATTTAATGA TTGAATCTCA GAAGGAACTG ATTGAGACCT TCCAAAAACA AGAAGACGAC 
TCTATGCAGC AGAAAGAAAT TCTGCAGCAT CTTAAAGGGG CTTTAAAGGA ACAAGAACAA 
GAAACTTTAT CTCTTAGAAA GCAGTGTGAG GCATTTAAAG AAAAGGAGGA AAAGCACAAA 
ACTGATCAAG TGATTCTTCA AGAAACAAAA ATCACTCTGA AAGAAAGAGA AAGAAAGATA 
GAGGTTTTAG AGAAAGCTGT CTCTAAGCTT CAACAACAAA ATGATGATAC GATGATGCAG 
ACTACAGCTA TAGTGCAAAA ACTACAATGT GCTGAATCTT CCTTAGCAGC TAGGGATCAA 
GAGATAGCAT CTCTGAAAGA GCATGTCCAG GAACTTCAGA AGCAGAAGGA GTCGGAAGCT 
AAACAGGTCA AAAGTCTACA GCAGGATCTA AACAAAATGA GCAAAACAAT GAAGAAGAAC 
AATTTGCAGA TTCTCAAACA GTCGGAGCAG ATTAATGTGT TTCATATTTG TGAAGAAAGT 
AGGAAAGTAG CACTAACATC TTGCCAGAAT CAAGTGAGTC TGCTTGAGGA AGTGGTGAGA 
AAGAGAGATG AAGACAATGA AACTCTTCTG CAAAAACTCC AGCACCAAGA AGAAGAACTG 
AAGACCTTGC AGAATCTCCA GCTTATGCTA TCTGAGAAGA AAGAGGAGAT TAAACGTCAC 
GGAGAGGAAG AGAAGTTCCC AGAAGAAGCC TTGCATGGGA GTGAATGGGA GACGAAGGCT 
CAAGGTGAGC AAAAAAAGTC AGAAGATGAG GAAATAAGAG GTCTTCATGA AGATCTCCAG 
TATGTTCAGC AGACTCTGAC AAAGAAGGAT GAAGAGATCA AATACCAGAG AGACAGAGTC 
AAGTATTTAG AGAAAACTCT GGCAGGGAAA GAAAAAGAGC TGAGGAGGCA GAGTGAACTC 
TTGAAACAAT TAACATCAGC TCTGCATTGG AAAGATGAAG GGGAGACTCT AAAGAAACAA 
ATCCAGAAAC TCCAAAAATG GGAGGAAGAA GAAGCAGAGA ACAGGAGGGT TCTCCAAGAG 
AGAGACCAAT TCTTGCAAAG GCAGAAGGAA CTAGCCCAAC AACTGGAAGA TGAGAGGAAA 
GTAAAGGGGG AAGAACTGAA GCGTGTGATT GCTATTTTGA AGCAGACTGA AAGTGGCGAA 
ATCGAATGGA AAGAGAAGGC ACAAGCACTG ACTCTTGCCC TTTCTAAGAG TGAAATGACC 
AATGGGACTT TGAGGGAAGA AATAGCCATT CTGCAGAGTA TGGTTTCAGA GAGGGACAAG 
GACCGGTTTC ATCTTCAGGA GGCTTTTGAA GGCGGTGAGA ACCTATCGTG GCTCTTGGAA 
AAGAAGCTCC TATGGCAGCG GCTGGAGTGT CTGCAGCAAG CAGTTGCAAG GCTGGAACAT 
GAAAAGTCTG AGCTGAAGCA ACTCAATGTT GAGCTCAAGA TAACTCTTGA GCAGGTGGAA 
CGTGAACGGA GGAGGCTGAA GAGAAGTTGT AGTCATCAGT CACAGCCAAA TGCAAACAGG 
TTTTCTCTCT CGGAATCTGA TGAGGACAAG ATGCCTGCTT CTAGAAAGGA GGAGGCTCGT 
GCTCTCTGCA GTCAATTAGC TGAGTTACAG AACCAGGTGA CTCTTCTGCA GCAGCAGTTG 
GCACAAGAAC GAAAATACAA GCAGGACTAT ATTGAGTGTT GTGCAAAAAC CAGTCAGGAG 
CTTTTGGACC TTCACCAAGA GCTGTCTTAC TCCCTGGAGG CTGTGATCAG GGAGCCCAAA 
GCTGCTGTTC TGGAGGCAGA GACTCAGAAG CTGGATCTAT CTCTGAACCA TACCTTGGCA 
CTAATATCTT TGGACTGCCG ATCTCCTGAG AGACAGCTGT TGCACTCAGC AGTGAGATCC 
ACCACAAGGG ATGGTCTGAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004947007.1
CDS	171..7334
Translation	MKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVTLLQQQLAQERKYKQDYIECCAKTSQELLDLHQELSYSLEAVIREPKAAVLEAETQKLDLSLNHTLALISLDCRSPERQLLHSAVRSTTRDGLR

Target ORF information:

RefSeq Version	XM_004946950.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004946950.2

ORF Insert Sequence:

ATGAAGACTG CCACTGACAG AGATTTGTCA GAGCTGAAGG CAGAGCAAAT GAGACTCTCT 
GGATCTATAC TTGTAAGCTG CTTCCGTTTG AACTCTGGCG TACAGCTCTG GGAGTCTGTC 
ACTTTGGGGA GGCCTGTCCT AAAGGATCAA GCACAGCAGC AAGTGGAAAG GGAAATAAAC 
CAGGAGGCTT TGGAAGTGAT GTGCTTACAA GTTGATGAGG ACCTGGAGAA GAAAGAGCTT 
CAAGATAGGG TGATGGAGCT CTCAGCCTTG CTTGTACAGT CTCAGAAACA AAATGAAGAG 
AAAGAGAAGA CCGTGAAAAC ACTTAACGAC ACTGTGGAGA TGCTAGAAGC AAGTCGGTTA 
GAGGTAGAAT ACGAAGCTTC ATTAACTAAA AATGCCAAAG AAGAGAACCT TGCCCTTCAG 
AAGCTAATCA AAGAGATAAC TGAGGTGGTG TTAGATGACA GTGACAGCAC AGTCGGCATT 
GTCCAGCGTC CAGAGTCTAG CAACGTCCTC TCTTGTCTGA GCTCCCTTGA TGCAGAGCAT 
GCCTTTGTGT TGGTTCAGGA AACACTGGCA AGGAGGCAAA AAGCAATACA GGCCCTAAAA 
GAAGAGCTTT CTACCAGGCA AGACTCTATC AGTTTTCTGC AGCACCAGCA CAGACAGCAG 
GAAGAGAAGT GCAAGAAGCT GCAGCAAAGG CTTGAGCAAC TGGAGGAGGA AAACAAGATG 
TCTAATAGCC ACCAGCAGCA CCTCAAGTCT CTGGTACTAG CGCTCAAGTG TGACTGTGAA 
AACCTAGAGA AAATCAGAGG AGAACTACAG CAAAAGCTGG AATTAAGTGA GCAAGAAGCC 
TCACGTCTGC GTCAGAGTAA CACTGAGCTG CAGCTGAAGG AAGATTCAGC CCAAGGGGAA 
AAGGTGGAGC AGCAGCTGGC AATGGAGAGA GCACATCATG ACCATGAGCT CGTTCTGAAG 
GACTTAGCTG CACTTGAAGA AAAACATTCG TTGTTACAGA ATGAATTGGT AGCCGCGAGG 
GAGAAGCTGG AGGAATCACA CCTTCAGAGG GATCTGCTGA AGCAAGAGAA ACATGAGCTT 
ACAGTGGCAC TGGAAAAGGC AGAGAAATCA GTAGCAGCAT TGACAGGGGC TCAGAATAAG 
TTGAATTCTG AAATAGCTGA CCTACATACT GCTGCAGCAA AGATGAGCAG TATTAATGAA 
GCTCTTGCAC TGGATAAAGT GGAACTGAAC AAGCTTGTGT TGCAGCTGGA ACAAGAGAAT 
GAACTTCTGT CAGATAAAGT GAATGAGATG GAGAGAGCAA AGGTCTCTGA ACAGGAGAAG 
ATAACCTTGT ATAAAAGAAC AAATGAAGAG CTTTGTGCAG AGAAATCCCA CCTGGAGAAG 
CTGCTGAAGA AAGCAGAGGA GCAACAGGAA GAGCTGCAGA TGCAACTGAG AATACTGGCA 
GAGGAAAAGG AAGAAACCCT GGAGAAACTC AACCAGGTTT ACCGCCAGCA AGAGTCAGCT 
AGCAGTGGTT TGGAGCAGTT GCGCCAACAG TCCTCTCGCC AAGAGGATGC AGTGGCCAAG 
GTGTCCAAAG AGAAGGAATT CCTGGTGCAT GAGAAGGCCG CCCTTGAGGT GCGACTGGCA 
GCTGTGGAAC GGGACAGACA AGGCCTTTCA GAGCAGCTGG CAGAGGCCAG GTCAGTGAAG 
GACACCCTGG AATCCAGCCT GTTTGAGGCT CAGCAGCAGT TATCTCACCT GGAGATCACC 
AAGAGTCAGC TTGAAATCCA GCTTCATACA GTCGTTCAGG CTAAGGAAGT GATACAAGGG 
GAAGTGAAGT GTCTTCAATA TGAGCTGGAA ACAGAGAGAT GTCTAATGAA GCAGGAACAG 
AAAAACATGG CACAACGGCT CATTCAGATA GAAGAGCAGC ATAACAATAC CCTGAAACTT 
CAGCAAACTG ATCATGAAGT GGAAATAAAC AAACTCCTAC AAGACCTGGC AAGTGAACGG 
GAGGGGCGCC AGTTAGAGCT ACAGGAGATG CTGGAGCTCT GGGAAAAGGA GAAAGCAGAG 
GCAGAAGGGC AGCATGAGAA GAAGCTGTTT CATATGAAGG AGAAAGTTGC TACCATGCAA 
GCTCAACAAG AAGAGGAACG AACCAGAGTT GAAAGTGCCA ATCAAGAGAT TTTGACAGAA 
AAGGAGAACG AGAAGAAAGC TTTATTAGAG ACACTACTCC AAACTCAGGG AGAACTAACA 
GAAGCCTGCC ATCAGCTTGA GCAACTTAGG CAGGAGGTGA AAGAGCAGCA GGAGTATGAG 
CAGAACATCA CAGAAAAACT GCAAGCAGAG CTACAGGAAA CTCACTGTAA AATCAAGATG 
GTGGAGAATA TGCACAAGGA AGAAATGGAA AACATCAAAG AAGAAAAGAA TATCCTTCTC 
CAGCAGAGGG ATGATCTACA AAAACAGGTA GAAGAGCTGA CATCTCAGCT AGCAGCCTCC 
GAAGAGTCCC ATCAAGCAAT TGGCCATAAA GCTCAGCAAG AGCTGAGTGA GGCACAAGAG 
TTGTCCAGGC AGAAGGTATT GGAGGTTGTG CACCTTCAGA AAATCTTGGA GGAGGAGAGG 
GGCCAATGGG AAGAGGTGGA ACATCAGAAC AAAGAGCTGC AGGTGTGCCT GCAGTCCTTA 
GAGGGGGAAA GGAGTCGATG GGAAGAAGTG GAACGTCACA ACACAGAATT TCAGGCTTCA 
TTGAAGGCCC TAGAGAGCGA AAGAGAAAGG CTATCTCTGT CTCTGGAACA GAAGGAATTG 
AGCCTCAAGA CCTTGGAAGA AAACAACCTT GTCCAGCAGA ACGAGGTGTC TAAACTACAT 
TCTGCTATTC AACAGGCTCA GCAGCTCCAC TCAGACCACA GAAGAGAAAT ACAAGAGCTC 
AACAACCAGG TACAGACACT ACAGGAAGTG GTGCTGGAGA AGGAAGCTAG CCTGGCAGCT 
CGGGAGAAGC AGCTATTACA GGATCTGGAG GAGTCTCGAG CAGGCGAACG GTGCTTGAGA 
GACTCTCTGC ACGTGCTAGA GGCTGAGATG GCTGAACTTC ATCTGAGGCT TTGTAGCACA 
GAGAACAGAG CAAAGGCATT GGCTACAGAG TGCCAGCAGG CTAATAATGC CCACTGTGAA 
GCCCAGTCTC AGCTGGACAA ATTGCACTTG GTTCTTCACC ACGTGCTGTG TGACAGCACT 
GATAAAAGCA GAGACTTAGT CGCTTGGAGT TCAGAACAGG ATCATGTCTG GGGCCTAACT 
GTTTCTCAGG CCAAGGATCT CCATGTAGAG CTCACAGTGG ACAGAGTGGC AGCAGCCCTG 
CAAGACCTGC GTCAGGACCT GAAGCAAACT CAGCAAGATC TGAATGATGC TGGGAAGAAA 
GTGCAGGACC TGGAACTGGA GCTGAGCAAG AGGCAAGCTG AGAGGGACCA CTTCAGTGCC 
CACAATCAGG AGCTACAGAA ACAGTTGGCT CAAAGCCAGG AAGAGACACA GATGGTAGAA 
GGGAAGAAGA ACTCTCTACA AGCAGCCTTG CAGGCAGAAG CTGCTGCTCT AAAGAAGGAG 
GCTATTACAC TACATCAAGA GGTGGCATCT TTGGAAAGGA AACTTGAGAG CGCTGAGAAG 
CAAAAAAAGG ATGTGCTGCA TGAACGGGAT AGACTGCAAG CAGTTGAAGA AAAACTGATG 
TGGGAGATAA AAATTCTTCA GGAGTCAGTC ACAGCCTCAG AAACACGAGC AAACACAGCA 
GCAGATATGA ATCACTCCCT TGAACAGGAA CTACAAACTA CATTGTCTGT CTTGAAAACA 
AAAAATAAGG AAGTGGATGC TCAATGGGAG AAAATCCAGA TGCTACAGAA AGAGGCAGCA 
GATGTAAAAG CTTTGCAGGA GAATCTTATT CAAGTGAATG CCATCCTGTC AAAGAGAGAG 
GGAGAAATGA AGTTATACCA GGAACAGATG AGAATGCTGG AAAACCAGAA AGAAATGCAT 
AAAACTACTC TAGATCAAGT TATTAAGGAC ATAAAAGAGA AAAAAGAGAA GACAGAATCC 
CAGCAAGAAC AAATACAGGA GTTAGAGAAG CAGCAAGAAC TGCAAAGGAC TGTTATAAGC 
AAAATGAGCA AAGACCTGGA AGACAGAGAT AAGGAGATCA GATCCCAGCA AGAAGAAATA 
TGGGAGCTGG AGAAGCAACA AGAACTGCAG AGGACTGTTG TAAGCAAGAT GACTAAAGAC 
CTGGCACACA GAGACCAGGA GATCCAATCC CAACAAGAAG AGATTCAGGA GCTAGAGAAG 
GAGCGAGAAT TGCAGAGGAC TGCTGCAAGT AAGATGAGCA AAGATCTAAA GGAGAGAGAT 
GAGAAGATAA GATCCCAGCA AGAACTGATA GAGGAGCTAG AGAAGCAGCA AGAACTGCAG 
AGGACTGCCT TAAGCAAAAT GAGCAAAAAC CTGGAGGAGA GAGACCAGGA GATCAAATCC 
CAGCAAGAAC TGATAGAGGA GCTAAAGAAG CAACAAGAAC TACAGAGGAC TGCTGTGAGC 
AAAATGAACA AAGACCTGGA GGAGAGAGAC CAGGAGATTA GATCCCAGCA AGAAGAGATA 
CAGGAACTGG AAAAGCAACG AGAACTGCAG AGGACTATTT TAAGCAAGAT GAGCAAAGAT 
CTGGAAGAGA AAGATCAGGT GATCAAGTTC CAGGAAGGGA AGGTAATGAT TCTAGAACAA 
CATGGTACAT CACAGGTGAG AAGTTTGCTA GTGGATCTTG ATCATATGAA GGGAAACTTG 
AAGGAGAAAA ACTTAGAGCT GATGTCTCTA AATCAGCAGA TCAAAGAACT GGAGATGGAG 
AGAGAAGAGG TGAAATCTTT GCACACAAGC CTTGAACAGC TTAGAGCAGT TCTTAGGGAT 
AGAGAGAATG AGTCTGACTC TCAAAGGGAT CAGTTAAGAC TCTTTCAGCA GTACAAGGAG 
CATCAAGAGG AGTACCTGCA AGAGCTCCAG GATAAAGTAG AAAAAATGAC ACTTTCCTTA 
TCTAAAAAAG ATCAAGAGCT CGAGTCACAG CAAAAGCAAA TCCAGGAAGC TGAAGAAGTC 
ATGGAAAAGA AGTTGAAGAC TGTTTGTGAC CAACTGGAGC AGACATTAGA AACCTTAAAA 
GAAAAGGAAA GACTCCTAGA CATCCAAAAG CAACAAACAA GGGAATATGA GGAAAAAACA 
GAACAGATGA ATGTCTTATG TAGAGACTTA GAATACACTA AGGCAATATT GAGAGAGAAG 
GATTTAATGA TTGAATCTCA GAAGGAACTG ATTGAGACCT TCCAAAAACA AGAAGACGAC 
TCTATGCAGC AGAAAGAAAT TCTGCAGCAT CTTAAAGGGG CTTTAAAGGA ACAAGAACAA 
GAAACTTTAT CTCTTAGAAA GCAGTGTGAG GCATTTAAAG AAAAGGAGGA AAAGCACAAA 
ACTGATCAAG TGATTCTTCA AGAAACAAAA ATCACTCTGA AAGAAAGAGA AAGAAAGATA 
GAGGTTTTAG AGAAAGCTGT CTCTAAGCTT CAACAACAAA ATGATGATAC GATGATGCAG 
ACTACAGCTA TAGTGCAAAA ACTACAATGT GCTGAATCTT CCTTAGCAGC TAGGGATCAA 
GAGATAGCAT CTCTGAAAGA GCATGTCCAG GAACTTCAGA AGCAGAAGGA GTCGGAAGCT 
AAACAGGTCA AAAGTCTACA GCAGGATCTA AACAAAATGA GCAAAACAAT GAAGAAGAAC 
AATTTGCAGA TTCTCAAACA GTCGGAGCAG ATTAATGTGT TTCATATTTG TGAAGAAAGT 
AGGAAAGTAG CACTAACATC TTGCCAGAAT CAAGTGAGTC TGCTTGAGGA AGTGGTGAGA 
AAGAGAGATG AAGACAATGA AACTCTTCTG CAAAAACTCC AGCACCAAGA AGAAGAACTG 
AAGACCTTGC AGAATCTCCA GCTTATGCTA TCTGAGAAGA AAGAGGAGAT TAAACGTCAC 
GGAGAGGAAG AGAAGTTCCC AGAAGAAGCC TTGCATGGGA GTGAATGGGA GACGAAGGCT 
CAAGGTGAGC AAAAAAAGTC AGAAGATGAG GAAATAAGAG GTCTTCATGA AGATCTCCAG 
TATGTTCAGC AGACTCTGAC AAAGAAGGAT GAAGAGATCA AATACCAGAG AGACAGAGTC 
AAGTATTTAG AGAAAACTCT GGCAGGGAAA GAAAAAGAGC TGAGGAGGCA GAGTGAACTC 
TTGAAACAAT TAACATCAGC TCTGCATTGG AAAGATGAAG GGGAGACTCT AAAGAAACAA 
ATCCAGAAAC TCCAAAAATG GGAGGAAGAA GAAGCAGAGA ACAGGAGGGT TCTCCAAGAG 
AGAGACCAAT TCTTGCAAAG GCAGAAGGAA CTAGCCCAAC AACTGGAAGA TGAGAGGAAA 
GTAAAGGGGG AAGAACTGAA GCGTGTGATT GCTATTTTGA AGCAGACTGA AAGTGGCGAA 
ATCGAATGGA AAGAGAAGGC ACAAGCACTG ACTCTTGCCC TTTCTAAGAG TGAAATGACC 
AATGGGACTT TGAGGGAAGA AATAGCCATT CTGCAGAGTA TGGTTTCAGA GAGGGACAAG 
GACCGGTTTC ATCTTCAGGA GGCTTTTGAA GGCGGTGAGA ACCTATCGTG GCTCTTGGAA 
AAGAAGCTCC TATGGCAGCG GCTGGAGTGT CTGCAGCAAG CAGTTGCAAG GCTGGAACAT 
GAAAAGTCTG AGCTGAAGCA ACTCAATGTT GAGCTCAAGA TAACTCTTGA GCAGGTGGAA 
CGTGAACGGA GGAGGCTGAA GAGAAGTTGT AGTCATCAGT CACAGCCAAA TGCAAACAGG 
TTTTCTCTCT CGGAATCTGA TGAGGACAAG ATGCCTGCTT CTAGAAAGGA GGAGGCTCGT 
GCTCTCTGCA GTCAATTAGC TGAGTTACAG AACCAGGTGA CTCTTCTGCA GCAGCAGTTG 
GCACAAGAAC GAAAATACAA GCAGGACTAT ATTGAGTGTT GTGCAAAAAC CAGTCAGGAG 
CTTTTGGACC TTCACCAAGA GCTGTCTTAC TCCCTGGAGG CTGTGATCAG GGAGCCCAAA 
GCTGCTGTTC TGGAGGCAGA GACTCAGAAG CTGGATCTAT CTCTGAACCA TACCTTGGCA 
CTAATATCTT TGGACTGCCG ATCTCCTGAG AGACAGCTGT TGCACTCAGC AGTGAGATCC 
ACCACAAGGG ATGGTCTGAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015296253.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151739.1 Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

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CloneID	OGa44661
Clone ID Related Accession (Same CDS sequence)	XM_015296253.1 , XM_015296253.2
Accession Version	XM_015296253.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455990.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGACTCT TCTGCAGCAG CAGTTGGCAC AAGAACGAAA ATACAAGCAG 
GACTATATTG AGTGTTGTGC AAAAACCAGT CAGGAGCTTT TGGACCTTCA CCAAGAGCTG 
TCTTACTCCC TGGAGGCTGT GATCAGGGAG CCCAAAGCTG CTGTTCTGGA GGCAGAGACT 
CAGAAGCTGG ATCTATCTCT GAACCATACC TTGGCACTAA TATCTTTGGA CTGCCGATCT 
CCTGAGAGAC AGCTGTTGCA CTCAGCAGTG AGATCCACCA CAAGGGATGG TCTGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151739.1
CDS	14..7693
Translation	MAAREAARGQACLRRRLHGAEEAQQQQAVLVRKLQEKLLQYRSWCRELEQRLEAGGVSVQGSLSYRWETTEDHSLEKALLQLEEEQQRCENLAEVNTLLREHLDKANEVNLALKEDVGKLTADWMRAREELELKESEWRSEREFYDSYLRGEHNRLLSLWRQVLTFRRHFLEMKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVTLLQQQLAQERKYKQDYIECCAKTSQELLDLHQELSYSLEAVIREPKAAVLEAETQKLDLSLNHTLALISLDCRSPERQLLHSAVRSTTRDGLR

Target ORF information:

RefSeq Version	XM_015296253.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296253.1

ORF Insert Sequence:

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGACTCT TCTGCAGCAG CAGTTGGCAC AAGAACGAAA ATACAAGCAG 
GACTATATTG AGTGTTGTGC AAAAACCAGT CAGGAGCTTT TGGACCTTCA CCAAGAGCTG 
TCTTACTCCC TGGAGGCTGT GATCAGGGAG CCCAAAGCTG CTGTTCTGGA GGCAGAGACT 
CAGAAGCTGG ATCTATCTCT GAACCATACC TTGGCACTAA TATCTTTGGA CTGCCGATCT 
CCTGAGAGAC AGCTGTTGCA CTCAGCAGTG AGATCCACCA CAAGGGATGG TCTGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015296253.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151739.1 Gallus gallus centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

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CloneID	OGa44661
Clone ID Related Accession (Same CDS sequence)	XM_015296253.1 , XM_015296253.2
Accession Version	XM_015296253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGACTCT TCTGCAGCAG CAGTTGGCAC AAGAACGAAA ATACAAGCAG 
GACTATATTG AGTGTTGTGC AAAAACCAGT CAGGAGCTTT TGGACCTTCA CCAAGAGCTG 
TCTTACTCCC TGGAGGCTGT GATCAGGGAG CCCAAAGCTG CTGTTCTGGA GGCAGAGACT 
CAGAAGCTGG ATCTATCTCT GAACCATACC TTGGCACTAA TATCTTTGGA CTGCCGATCT 
CCTGAGAGAC AGCTGTTGCA CTCAGCAGTG AGATCCACCA CAAGGGATGG TCTGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151739.1
CDS	16..7695
Translation	MAAREAARGQACLRRRLHGAEEAQQQQAVLVRKLQEKLLQYRSWCRELEQRLEAGGVSVQGSLSYRWETTEDHSLEKALLQLEEEQQRCENLAEVNTLLREHLDKANEVNLALKEDVGKLTADWMRAREELELKESEWRSEREFYDSYLRGEHNRLLSLWRQVLTFRRHFLEMKTATDRDLSELKAEQMRLSGSILVSCFRLNSGVQLWESVTLGRPVLKDQAQQQVEREINQEALEVMCLQVDEDLEKKELQDRVMELSALLVQSQKQNEEKEKTVKTLNDTVEMLEASRLEVEYEASLTKNAKEENLALQKLIKEITEVVLDDSDSTVGIVQRPESSNVLSCLSSLDAEHAFVLVQETLARRQKAIQALKEELSTRQDSISFLQHQHRQQEEKCKKLQQRLEQLEEENKMSNSHQQHLKSLVLALKCDCENLEKIRGELQQKLELSEQEASRLRQSNTELQLKEDSAQGEKVEQQLAMERAHHDHELVLKDLAALEEKHSLLQNELVAAREKLEESHLQRDLLKQEKHELTVALEKAEKSVAALTGAQNKLNSEIADLHTAAAKMSSINEALALDKVELNKLVLQLEQENELLSDKVNEMERAKVSEQEKITLYKRTNEELCAEKSHLEKLLKKAEEQQEELQMQLRILAEEKEETLEKLNQVYRQQESASSGLEQLRQQSSRQEDAVAKVSKEKEFLVHEKAALEVRLAAVERDRQGLSEQLAEARSVKDTLESSLFEAQQQLSHLEITKSQLEIQLHTVVQAKEVIQGEVKCLQYELETERCLMKQEQKNMAQRLIQIEEQHNNTLKLQQTDHEVEINKLLQDLASEREGRQLELQEMLELWEKEKAEAEGQHEKKLFHMKEKVATMQAQQEEERTRVESANQEILTEKENEKKALLETLLQTQGELTEACHQLEQLRQEVKEQQEYEQNITEKLQAELQETHCKIKMVENMHKEEMENIKEEKNILLQQRDDLQKQVEELTSQLAASEESHQAIGHKAQQELSEAQELSRQKVLEVVHLQKILEEERGQWEEVEHQNKELQVCLQSLEGERSRWEEVERHNTEFQASLKALESERERLSLSLEQKELSLKTLEENNLVQQNEVSKLHSAIQQAQQLHSDHRREIQELNNQVQTLQEVVLEKEASLAAREKQLLQDLEESRAGERCLRDSLHVLEAEMAELHLRLCSTENRAKALATECQQANNAHCEAQSQLDKLHLVLHHVLCDSTDKSRDLVAWSSEQDHVWGLTVSQAKDLHVELTVDRVAAALQDLRQDLKQTQQDLNDAGKKVQDLELELSKRQAERDHFSAHNQELQKQLAQSQEETQMVEGKKNSLQAALQAEAAALKKEAITLHQEVASLERKLESAEKQKKDVLHERDRLQAVEEKLMWEIKILQESVTASETRANTAADMNHSLEQELQTTLSVLKTKNKEVDAQWEKIQMLQKEAADVKALQENLIQVNAILSKREGEMKLYQEQMRMLENQKEMHKTTLDQVIKDIKEKKEKTESQQEQIQELEKQQELQRTVISKMSKDLEDRDKEIRSQQEEIWELEKQQELQRTVVSKMTKDLAHRDQEIQSQQEEIQELEKERELQRTAASKMSKDLKERDEKIRSQQELIEELEKQQELQRTALSKMSKNLEERDQEIKSQQELIEELKKQQELQRTAVSKMNKDLEERDQEIRSQQEEIQELEKQRELQRTILSKMSKDLEEKDQVIKFQEGKVMILEQHGTSQVRSLLVDLDHMKGNLKEKNLELMSLNQQIKELEMEREEVKSLHTSLEQLRAVLRDRENESDSQRDQLRLFQQYKEHQEEYLQELQDKVEKMTLSLSKKDQELESQQKQIQEAEEVMEKKLKTVCDQLEQTLETLKEKERLLDIQKQQTREYEEKTEQMNVLCRDLEYTKAILREKDLMIESQKELIETFQKQEDDSMQQKEILQHLKGALKEQEQETLSLRKQCEAFKEKEEKHKTDQVILQETKITLKERERKIEVLEKAVSKLQQQNDDTMMQTTAIVQKLQCAESSLAARDQEIASLKEHVQELQKQKESEAKQVKSLQQDLNKMSKTMKKNNLQILKQSEQINVFHICEESRKVALTSCQNQVSLLEEVVRKRDEDNETLLQKLQHQEEELKTLQNLQLMLSEKKEEIKRHGEEEKFPEEALHGSEWETKAQGEQKKSEDEEIRGLHEDLQYVQQTLTKKDEEIKYQRDRVKYLEKTLAGKEKELRRQSELLKQLTSALHWKDEGETLKKQIQKLQKWEEEEAENRRVLQERDQFLQRQKELAQQLEDERKVKGEELKRVIAILKQTESGEIEWKEKAQALTLALSKSEMTNGTLREEIAILQSMVSERDKDRFHLQEAFEGGENLSWLLEKKLLWQRLECLQQAVARLEHEKSELKQLNVELKITLEQVERERRRLKRSCSHQSQPNANRFSLSESDEDKMPASRKEEARALCSQLAELQNQVTLLQQQLAQERKYKQDYIECCAKTSQELLDLHQELSYSLEAVIREPKAAVLEAETQKLDLSLNHTLALISLDCRSPERQLLHSAVRSTTRDGLR

Target ORF information:

RefSeq Version	XM_015296253.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296253.2

ORF Insert Sequence:

ATGGCGGCGA GGGAGGCGGC GCGGGGCCAG GCCTGCCTGC GGCGTCGGCT GCACGGAGCG 
GAGGAGGCGC AGCAGCAGCA AGCGGTGCTG GTGCGGAAGC TGCAGGAGAA GCTGCTGCAG 
TATCGGAGCT GGTGTCGTGA GCTGGAGCAG CGACTTGAAG CAGGAGGGGT GAGTGTGCAG 
GGATCCCTTT CATACAGATG GGAAACCACT GAAGACCACA GCCTGGAGAA GGCACTCCTC 
CAGTTAGAAG AAGAGCAGCA AAGGTGTGAG AATCTGGCAG AAGTGAATAC CCTCCTGCGG 
GAACACCTAG ATAAAGCAAA TGAGGTGAAT TTAGCCCTTA AAGAAGATGT TGGAAAACTG 
ACAGCAGATT GGATGAGGGC CCGAGAGGAG CTGGAATTGA AGGAGAGTGA ATGGCGCAGT 
GAGCGTGAGT TTTATGACAG CTACTTAAGG GGTGAACATA ACCGTCTTCT GAGCCTATGG 
CGTCAGGTGC TGACTTTCCG CCGTCACTTC CTGGAAATGA AGACTGCCAC TGACAGAGAT 
TTGTCAGAGC TGAAGGCAGA GCAAATGAGA CTCTCTGGAT CTATACTTGT AAGCTGCTTC 
CGTTTGAACT CTGGCGTACA GCTCTGGGAG TCTGTCACTT TGGGGAGGCC TGTCCTAAAG 
GATCAAGCAC AGCAGCAAGT GGAAAGGGAA ATAAACCAGG AGGCTTTGGA AGTGATGTGC 
TTACAAGTTG ATGAGGACCT GGAGAAGAAA GAGCTTCAAG ATAGGGTGAT GGAGCTCTCA 
GCCTTGCTTG TACAGTCTCA GAAACAAAAT GAAGAGAAAG AGAAGACCGT GAAAACACTT 
AACGACACTG TGGAGATGCT AGAAGCAAGT CGGTTAGAGG TAGAATACGA AGCTTCATTA 
ACTAAAAATG CCAAAGAAGA GAACCTTGCC CTTCAGAAGC TAATCAAAGA GATAACTGAG 
GTGGTGTTAG ATGACAGTGA CAGCACAGTC GGCATTGTCC AGCGTCCAGA GTCTAGCAAC 
GTCCTCTCTT GTCTGAGCTC CCTTGATGCA GAGCATGCCT TTGTGTTGGT TCAGGAAACA 
CTGGCAAGGA GGCAAAAAGC AATACAGGCC CTAAAAGAAG AGCTTTCTAC CAGGCAAGAC 
TCTATCAGTT TTCTGCAGCA CCAGCACAGA CAGCAGGAAG AGAAGTGCAA GAAGCTGCAG 
CAAAGGCTTG AGCAACTGGA GGAGGAAAAC AAGATGTCTA ATAGCCACCA GCAGCACCTC 
AAGTCTCTGG TACTAGCGCT CAAGTGTGAC TGTGAAAACC TAGAGAAAAT CAGAGGAGAA 
CTACAGCAAA AGCTGGAATT AAGTGAGCAA GAAGCCTCAC GTCTGCGTCA GAGTAACACT 
GAGCTGCAGC TGAAGGAAGA TTCAGCCCAA GGGGAAAAGG TGGAGCAGCA GCTGGCAATG 
GAGAGAGCAC ATCATGACCA TGAGCTCGTT CTGAAGGACT TAGCTGCACT TGAAGAAAAA 
CATTCGTTGT TACAGAATGA ATTGGTAGCC GCGAGGGAGA AGCTGGAGGA ATCACACCTT 
CAGAGGGATC TGCTGAAGCA AGAGAAACAT GAGCTTACAG TGGCACTGGA AAAGGCAGAG 
AAATCAGTAG CAGCATTGAC AGGGGCTCAG AATAAGTTGA ATTCTGAAAT AGCTGACCTA 
CATACTGCTG CAGCAAAGAT GAGCAGTATT AATGAAGCTC TTGCACTGGA TAAAGTGGAA 
CTGAACAAGC TTGTGTTGCA GCTGGAACAA GAGAATGAAC TTCTGTCAGA TAAAGTGAAT 
GAGATGGAGA GAGCAAAGGT CTCTGAACAG GAGAAGATAA CCTTGTATAA AAGAACAAAT 
GAAGAGCTTT GTGCAGAGAA ATCCCACCTG GAGAAGCTGC TGAAGAAAGC AGAGGAGCAA 
CAGGAAGAGC TGCAGATGCA ACTGAGAATA CTGGCAGAGG AAAAGGAAGA AACCCTGGAG 
AAACTCAACC AGGTTTACCG CCAGCAAGAG TCAGCTAGCA GTGGTTTGGA GCAGTTGCGC 
CAACAGTCCT CTCGCCAAGA GGATGCAGTG GCCAAGGTGT CCAAAGAGAA GGAATTCCTG 
GTGCATGAGA AGGCCGCCCT TGAGGTGCGA CTGGCAGCTG TGGAACGGGA CAGACAAGGC 
CTTTCAGAGC AGCTGGCAGA GGCCAGGTCA GTGAAGGACA CCCTGGAATC CAGCCTGTTT 
GAGGCTCAGC AGCAGTTATC TCACCTGGAG ATCACCAAGA GTCAGCTTGA AATCCAGCTT 
CATACAGTCG TTCAGGCTAA GGAAGTGATA CAAGGGGAAG TGAAGTGTCT TCAATATGAG 
CTGGAAACAG AGAGATGTCT AATGAAGCAG GAACAGAAAA ACATGGCACA ACGGCTCATT 
CAGATAGAAG AGCAGCATAA CAATACCCTG AAACTTCAGC AAACTGATCA TGAAGTGGAA 
ATAAACAAAC TCCTACAAGA CCTGGCAAGT GAACGGGAGG GGCGCCAGTT AGAGCTACAG 
GAGATGCTGG AGCTCTGGGA AAAGGAGAAA GCAGAGGCAG AAGGGCAGCA TGAGAAGAAG 
CTGTTTCATA TGAAGGAGAA AGTTGCTACC ATGCAAGCTC AACAAGAAGA GGAACGAACC 
AGAGTTGAAA GTGCCAATCA AGAGATTTTG ACAGAAAAGG AGAACGAGAA GAAAGCTTTA 
TTAGAGACAC TACTCCAAAC TCAGGGAGAA CTAACAGAAG CCTGCCATCA GCTTGAGCAA 
CTTAGGCAGG AGGTGAAAGA GCAGCAGGAG TATGAGCAGA ACATCACAGA AAAACTGCAA 
GCAGAGCTAC AGGAAACTCA CTGTAAAATC AAGATGGTGG AGAATATGCA CAAGGAAGAA 
ATGGAAAACA TCAAAGAAGA AAAGAATATC CTTCTCCAGC AGAGGGATGA TCTACAAAAA 
CAGGTAGAAG AGCTGACATC TCAGCTAGCA GCCTCCGAAG AGTCCCATCA AGCAATTGGC 
CATAAAGCTC AGCAAGAGCT GAGTGAGGCA CAAGAGTTGT CCAGGCAGAA GGTATTGGAG 
GTTGTGCACC TTCAGAAAAT CTTGGAGGAG GAGAGGGGCC AATGGGAAGA GGTGGAACAT 
CAGAACAAAG AGCTGCAGGT GTGCCTGCAG TCCTTAGAGG GGGAAAGGAG TCGATGGGAA 
GAAGTGGAAC GTCACAACAC AGAATTTCAG GCTTCATTGA AGGCCCTAGA GAGCGAAAGA 
GAAAGGCTAT CTCTGTCTCT GGAACAGAAG GAATTGAGCC TCAAGACCTT GGAAGAAAAC 
AACCTTGTCC AGCAGAACGA GGTGTCTAAA CTACATTCTG CTATTCAACA GGCTCAGCAG 
CTCCACTCAG ACCACAGAAG AGAAATACAA GAGCTCAACA ACCAGGTACA GACACTACAG 
GAAGTGGTGC TGGAGAAGGA AGCTAGCCTG GCAGCTCGGG AGAAGCAGCT ATTACAGGAT 
CTGGAGGAGT CTCGAGCAGG CGAACGGTGC TTGAGAGACT CTCTGCACGT GCTAGAGGCT 
GAGATGGCTG AACTTCATCT GAGGCTTTGT AGCACAGAGA ACAGAGCAAA GGCATTGGCT 
ACAGAGTGCC AGCAGGCTAA TAATGCCCAC TGTGAAGCCC AGTCTCAGCT GGACAAATTG 
CACTTGGTTC TTCACCACGT GCTGTGTGAC AGCACTGATA AAAGCAGAGA CTTAGTCGCT 
TGGAGTTCAG AACAGGATCA TGTCTGGGGC CTAACTGTTT CTCAGGCCAA GGATCTCCAT 
GTAGAGCTCA CAGTGGACAG AGTGGCAGCA GCCCTGCAAG ACCTGCGTCA GGACCTGAAG 
CAAACTCAGC AAGATCTGAA TGATGCTGGG AAGAAAGTGC AGGACCTGGA ACTGGAGCTG 
AGCAAGAGGC AAGCTGAGAG GGACCACTTC AGTGCCCACA ATCAGGAGCT ACAGAAACAG 
TTGGCTCAAA GCCAGGAAGA GACACAGATG GTAGAAGGGA AGAAGAACTC TCTACAAGCA 
GCCTTGCAGG CAGAAGCTGC TGCTCTAAAG AAGGAGGCTA TTACACTACA TCAAGAGGTG 
GCATCTTTGG AAAGGAAACT TGAGAGCGCT GAGAAGCAAA AAAAGGATGT GCTGCATGAA 
CGGGATAGAC TGCAAGCAGT TGAAGAAAAA CTGATGTGGG AGATAAAAAT TCTTCAGGAG 
TCAGTCACAG CCTCAGAAAC ACGAGCAAAC ACAGCAGCAG ATATGAATCA CTCCCTTGAA 
CAGGAACTAC AAACTACATT GTCTGTCTTG AAAACAAAAA ATAAGGAAGT GGATGCTCAA 
TGGGAGAAAA TCCAGATGCT ACAGAAAGAG GCAGCAGATG TAAAAGCTTT GCAGGAGAAT 
CTTATTCAAG TGAATGCCAT CCTGTCAAAG AGAGAGGGAG AAATGAAGTT ATACCAGGAA 
CAGATGAGAA TGCTGGAAAA CCAGAAAGAA ATGCATAAAA CTACTCTAGA TCAAGTTATT 
AAGGACATAA AAGAGAAAAA AGAGAAGACA GAATCCCAGC AAGAACAAAT ACAGGAGTTA 
GAGAAGCAGC AAGAACTGCA AAGGACTGTT ATAAGCAAAA TGAGCAAAGA CCTGGAAGAC 
AGAGATAAGG AGATCAGATC CCAGCAAGAA GAAATATGGG AGCTGGAGAA GCAACAAGAA 
CTGCAGAGGA CTGTTGTAAG CAAGATGACT AAAGACCTGG CACACAGAGA CCAGGAGATC 
CAATCCCAAC AAGAAGAGAT TCAGGAGCTA GAGAAGGAGC GAGAATTGCA GAGGACTGCT 
GCAAGTAAGA TGAGCAAAGA TCTAAAGGAG AGAGATGAGA AGATAAGATC CCAGCAAGAA 
CTGATAGAGG AGCTAGAGAA GCAGCAAGAA CTGCAGAGGA CTGCCTTAAG CAAAATGAGC 
AAAAACCTGG AGGAGAGAGA CCAGGAGATC AAATCCCAGC AAGAACTGAT AGAGGAGCTA 
AAGAAGCAAC AAGAACTACA GAGGACTGCT GTGAGCAAAA TGAACAAAGA CCTGGAGGAG 
AGAGACCAGG AGATTAGATC CCAGCAAGAA GAGATACAGG AACTGGAAAA GCAACGAGAA 
CTGCAGAGGA CTATTTTAAG CAAGATGAGC AAAGATCTGG AAGAGAAAGA TCAGGTGATC 
AAGTTCCAGG AAGGGAAGGT AATGATTCTA GAACAACATG GTACATCACA GGTGAGAAGT 
TTGCTAGTGG ATCTTGATCA TATGAAGGGA AACTTGAAGG AGAAAAACTT AGAGCTGATG 
TCTCTAAATC AGCAGATCAA AGAACTGGAG ATGGAGAGAG AAGAGGTGAA ATCTTTGCAC 
ACAAGCCTTG AACAGCTTAG AGCAGTTCTT AGGGATAGAG AGAATGAGTC TGACTCTCAA 
AGGGATCAGT TAAGACTCTT TCAGCAGTAC AAGGAGCATC AAGAGGAGTA CCTGCAAGAG 
CTCCAGGATA AAGTAGAAAA AATGACACTT TCCTTATCTA AAAAAGATCA AGAGCTCGAG 
TCACAGCAAA AGCAAATCCA GGAAGCTGAA GAAGTCATGG AAAAGAAGTT GAAGACTGTT 
TGTGACCAAC TGGAGCAGAC ATTAGAAACC TTAAAAGAAA AGGAAAGACT CCTAGACATC 
CAAAAGCAAC AAACAAGGGA ATATGAGGAA AAAACAGAAC AGATGAATGT CTTATGTAGA 
GACTTAGAAT ACACTAAGGC AATATTGAGA GAGAAGGATT TAATGATTGA ATCTCAGAAG 
GAACTGATTG AGACCTTCCA AAAACAAGAA GACGACTCTA TGCAGCAGAA AGAAATTCTG 
CAGCATCTTA AAGGGGCTTT AAAGGAACAA GAACAAGAAA CTTTATCTCT TAGAAAGCAG 
TGTGAGGCAT TTAAAGAAAA GGAGGAAAAG CACAAAACTG ATCAAGTGAT TCTTCAAGAA 
ACAAAAATCA CTCTGAAAGA AAGAGAAAGA AAGATAGAGG TTTTAGAGAA AGCTGTCTCT 
AAGCTTCAAC AACAAAATGA TGATACGATG ATGCAGACTA CAGCTATAGT GCAAAAACTA 
CAATGTGCTG AATCTTCCTT AGCAGCTAGG GATCAAGAGA TAGCATCTCT GAAAGAGCAT 
GTCCAGGAAC TTCAGAAGCA GAAGGAGTCG GAAGCTAAAC AGGTCAAAAG TCTACAGCAG 
GATCTAAACA AAATGAGCAA AACAATGAAG AAGAACAATT TGCAGATTCT CAAACAGTCG 
GAGCAGATTA ATGTGTTTCA TATTTGTGAA GAAAGTAGGA AAGTAGCACT AACATCTTGC 
CAGAATCAAG TGAGTCTGCT TGAGGAAGTG GTGAGAAAGA GAGATGAAGA CAATGAAACT 
CTTCTGCAAA AACTCCAGCA CCAAGAAGAA GAACTGAAGA CCTTGCAGAA TCTCCAGCTT 
ATGCTATCTG AGAAGAAAGA GGAGATTAAA CGTCACGGAG AGGAAGAGAA GTTCCCAGAA 
GAAGCCTTGC ATGGGAGTGA ATGGGAGACG AAGGCTCAAG GTGAGCAAAA AAAGTCAGAA 
GATGAGGAAA TAAGAGGTCT TCATGAAGAT CTCCAGTATG TTCAGCAGAC TCTGACAAAG 
AAGGATGAAG AGATCAAATA CCAGAGAGAC AGAGTCAAGT ATTTAGAGAA AACTCTGGCA 
GGGAAAGAAA AAGAGCTGAG GAGGCAGAGT GAACTCTTGA AACAATTAAC ATCAGCTCTG 
CATTGGAAAG ATGAAGGGGA GACTCTAAAG AAACAAATCC AGAAACTCCA AAAATGGGAG 
GAAGAAGAAG CAGAGAACAG GAGGGTTCTC CAAGAGAGAG ACCAATTCTT GCAAAGGCAG 
AAGGAACTAG CCCAACAACT GGAAGATGAG AGGAAAGTAA AGGGGGAAGA ACTGAAGCGT 
GTGATTGCTA TTTTGAAGCA GACTGAAAGT GGCGAAATCG AATGGAAAGA GAAGGCACAA 
GCACTGACTC TTGCCCTTTC TAAGAGTGAA ATGACCAATG GGACTTTGAG GGAAGAAATA 
GCCATTCTGC AGAGTATGGT TTCAGAGAGG GACAAGGACC GGTTTCATCT TCAGGAGGCT 
TTTGAAGGCG GTGAGAACCT ATCGTGGCTC TTGGAAAAGA AGCTCCTATG GCAGCGGCTG 
GAGTGTCTGC AGCAAGCAGT TGCAAGGCTG GAACATGAAA AGTCTGAGCT GAAGCAACTC 
AATGTTGAGC TCAAGATAAC TCTTGAGCAG GTGGAACGTG AACGGAGGAG GCTGAAGAGA 
AGTTGTAGTC ATCAGTCACA GCCAAATGCA AACAGGTTTT CTCTCTCGGA ATCTGATGAG 
GACAAGATGC CTGCTTCTAG AAAGGAGGAG GCTCGTGCTC TCTGCAGTCA ATTAGCTGAG 
TTACAGAACC AGGTGACTCT TCTGCAGCAG CAGTTGGCAC AAGAACGAAA ATACAAGCAG 
GACTATATTG AGTGTTGTGC AAAAACCAGT CAGGAGCTTT TGGACCTTCA CCAAGAGCTG 
TCTTACTCCC TGGAGGCTGT GATCAGGGAG CCCAAAGCTG CTGTTCTGGA GGCAGAGACT 
CAGAAGCTGG ATCTATCTCT GAACCATACC TTGGCACTAA TATCTTTGGA CTGCCGATCT 
CCTGAGAGAC AGCTGTTGCA CTCAGCAGTG AGATCCACCA CAAGGGATGG TCTGAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CEP250 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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