FGA cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	FGA
Entrez Gene ID	396307
Full Name	fibrinogen alpha chain
General protein information	Preferred Names fibrinogen alpha chain Names fibrinogen alpha chain fibrinogen alpha subunit fibrinogen alpha-E subunit pre-fibrinogen alpha subunit
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271911.1	NP_001258840.1	fibrinogen alpha chain isoform 1 precursor
NM_205356.2	NP_990687.2	fibrinogen alpha chain isoform 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	FGA	NP_000499.1
Macaca mulatta (Rhesus monkey)	FGA	XP_001088855.2
Canis lupus familiaris (dog)	FGA	XP_532697.2
Rattus norvegicus (Norway rat)	Fga	NP_001008724.1
Danio rerio (zebrafish)	fga	NP_001181918.1
Mus musculus (house mouse)	Fga	NP_001104518.1
Gallus gallus (chicken)	FGA	NP_001258840.1
Pan troglodytes (chimpanzee)	FGA	XP_001136067.1
Xenopus tropicalis (tropical clawed frog)	fga	XP_002933535.2

Gene Ontology
Bibliography

Related articles in PubMed

Substitution of the human αC region with the analogous chicken domain generates a fibrinogen with severely impaired lateral aggregation: fibrin monomers assemble into protofibrils but protofibrils do not assemble into fibers.
Ping L, Huang L, Cardinali B, Profumo A, Gorkun OV, Lord ST
Biochemistry50(42)9066-75(2011 Oct)

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Carre W, Wang X, Porter TE, Nys Y, Tang J, Bernberg E, Morgan R, Burnside J, Aggrey SE, Simon J, Cogburn LA
Physiological genomics25(3)514-24(2006 May)

A comprehensive collection of chicken cDNAs.
Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, Fong WT, Tickle C, Brown WR, Wilson SA, Hubbard SJ
Current biology : CB12(22)1965-9(2002 Nov)

Crystal structure of native chicken fibrinogen at 2.7 A resolution.
Yang Z, Kollman JM, Pandi L, Doolittle RF
Biochemistry40(42)12515-23(2001 Oct)

Crystal structure of native chicken fibrinogen at 5.5-A resolution.
Yang Z, Mochalkin I, Veerapandian L, Riley M, Doolittle RF
Proceedings of the National Academy of Sciences of the United States of America97(8)3907-12(2000 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FGA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00028	NM_001271911.1 Latest version!	Gallus gallus fibrinogen alpha chain (FGA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa00158	NM_205356.2 Latest version!	Gallus gallus fibrinogen alpha chain (FGA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FGA ( NM_001271911.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001258840.1 Gallus gallus fibrinogen alpha chain (FGA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa00028
Clone ID Related Accession (Same CDS sequence)	NM_001271911.1
Accession Version	NM_001271911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Gallus gallus(chicken)
Product	fibrinogen alpha chain isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG641954.1, AADN05000019.1, BU251255.1, BU124839.1 and BU125048.1. On Dec 13, 2012 this sequence version replaced XM_001232366.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcripts of different breeds and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201370 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGATACCAG TGACGATACT CTGTGTGTTG CTCTGCCTCA ATTTAGCTTG GGCTCAGGAT 
GGGAAGACCA CCTTTGAAAA GGAGGGCGGA GGCGGGCGTG GCCCCAGGAT CCTAGAGAAC 
ATGCACGAGT CGTCCTGCAA GTATGAGAAG AACTGGCCTA TCTGTGTGGA TGATGACTGG 
GGTACAAAAT GTCCATCAGG CTGCAGAATG CAAGGAATAA TTGACGACAC AGACCAGAAT 
TACAGTCAAA GAATAGACAA TATTAGGCAG CAACTCGCAG ACAGTCAAAA CAAGTATAAA 
ACATCCAATC GGGTAATTGT GGAAACCATA AATATACTAA AGCCTGGTCT GGAGGGTGCC 
CAGCAGCTCG ATGAGAATTA CGGTCATGTG TCAACAGAAC TGAGGAGGAG AATTGTGACA 
TTAAAGCAGC GAGTTGCCAC TCAAGTGAAC AGAATTAAAG CTCTGCAGAA CAGCATCCAG 
GAGCAGGTGG TGGAAATGAA GCGCTTGGAG GTGGACATTG ATATTAAGAT ACGAGCTTGC 
AAAGGGAGCT GTGCTAGAAG TTTTGATTAC CAGGTGGACA AAGAAGGCTA TGACAACATC 
CAGAAGCATC TTACCCAGGC CAGCTCCATT GATATGCACC CAGACTTTCA AACAACCACC 
TTGAGCACAC TGAAAATGAG GCCTCTTAAG GACTCGAATG TTCCTGAACA TTTTAAGCTC 
AAGCCTTCAC CAGAAATGCA AGCTATGAGT GCATTTAATA ACATCAAACA GATGCAAGTG 
GTATTAGAAA GACCAGAAAC AGACCACGTG GCAGAAGCAA GGGGGGATTC TTCACCTTCC 
CACACTGGCA AATTAATTAC TTCTTCTCAC AGGAGAGAGA GCCCTAGCCT GGTAGACAAA 
ACCTCCTCTG CCTCCTCTGT CCATAGATGC ACCAGAACTG TCACCAAGAA AGTCATTAGT 
GGCCCTGATG GTCCTAGAGA AGAAATAGTT GAAAAAATGG TTTCCTCTGA TGGCTCAGAC 
TGCTCCCATC TACAAGGAGG ACGAGAAGGA AGCACGTACC ATTTTAGTGG GACAGGTGAC 
TTTCACAAGC TAGACAGGCT TCTGCCAGAT CTAGAGTCAT TCTTCACCCA TGACTCTGTA 
TCCACTAGTA GTAGGCACTC AATTGGCTCA AGCACCAGCA GCCATGTGAC TGGGGCAGGC 
AGCAGCCACC TAGGCACTGG AGGGAAAGAC AAATTCACAG ATTTAGGGGA GGAGGAAGAA 
GATGACTTTG GAGGACTTCA GCCATCTGGA TTTGCTGCTG GCAGTGCGAG TCACTCCAAG 
ACTGTACTGA CCAGCTCCTC TTCTAGTTTC AACAAGGGAG GCTCCACTTT TGAAACCAAG 
TCACTAAAGA CCCGTGAAAC ATCTGAGCAG CTAGGTGGGG TGCAACATGA TCAGAGTGCA 
GAGGACACCC CAGACTTTAA GGCGCGCAGC TTCAGACCAG CAGCAATGAG TACAAGGAGA 
TCCTACAATG GGAAAGACTG CGATGATATC CGCCAGAAAC ACACTTCTGG TGCCAAAAGT 
GGCATTTTCA AAATCAAGCC AGAGGGATCC AATAAGGTTT TGTCAGTTTA TTGCGACCAA 
GAGACCACTT TGGGAGGATG GCTATTGATC CAACAGAGAA TGGATGGATC AGTGAATTTT 
AACCGTACGT GGCAAGACTA CAGGAGAGGT TTCGGCAGCG TGGATGGCAA AGGGCAAGGA 
GAGTTGTGGC TGGGCAATGA GAATATCCAC TTGCTGACTC AGAACGATAC TCTGCTTCGA 
GTAGAGTTAG AGGACTGGGA TGGAAATGCT GCATATGCAG AGTACATCGT GCAGGTAGGG 
ACTGAAGCAG AAGGCTATGC CCTGACTGTG TCCTCCTATG AGGGGACTGC TGGGGATGCT 
CTGGTTGCTG GCTGGCTGGA GGAGGGCTCT GAGTACACAT CCCATGCCCA GATGCAGTTC 
AGCACTTTTG ACCGGGACCA GGACCACTGG GAGGAGAGCT GTGCAGAGGT GTACGGGGGT 
GGCTGGTGGT ACAACAGCTG CCAGGCAGCC AACCTCAATG GCATTTACTA TCCAGGGGGC 
CATTATGACC CCAGGTACAA TGTTCCATAT GAGATCGAGA ATGGAGTAGT CTGGATACCA 
TTTAGAGCCT CCGATTATTC CCTCAAAGTT GTTAGAATGA AAATCAGACC CCTGGAAACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258840.1
CDS	28..2253
Translation	MIPVTILCVLLCLNLAWAQDGKTTFEKEGGGGRGPRILENMHESSCKYEKNWPICVDDDWGTKCPSGCRMQGIIDDTDQNYSQRIDNIRQQLADSQNKYKTSNRVIVETINILKPGLEGAQQLDENYGHVSTELRRRIVTLKQRVATQVNRIKALQNSIQEQVVEMKRLEVDIDIKIRACKGSCARSFDYQVDKEGYDNIQKHLTQASSIDMHPDFQTTTLSTLKMRPLKDSNVPEHFKLKPSPEMQAMSAFNNIKQMQVVLERPETDHVAEARGDSSPSHTGKLITSSHRRESPSLVDKTSSASSVHRCTRTVTKKVISGPDGPREEIVEKMVSSDGSDCSHLQGGREGSTYHFSGTGDFHKLDRLLPDLESFFTHDSVSTSSRHSIGSSTSSHVTGAGSSHLGTGGKDKFTDLGEEEEDDFGGLQPSGFAAGSASHSKTVLTSSSSSFNKGGSTFETKSLKTRETSEQLGGVQHDQSAEDTPDFKARSFRPAAMSTRRSYNGKDCDDIRQKHTSGAKSGIFKIKPEGSNKVLSVYCDQETTLGGWLLIQQRMDGSVNFNRTWQDYRRGFGSVDGKGQGELWLGNENIHLLTQNDTLLRVELEDWDGNAAYAEYIVQVGTEAEGYALTVSSYEGTAGDALVAGWLEEGSEYTSHAQMQFSTFDRDQDHWEESCAEVYGGGWWYNSCQAANLNGIYYPGGHYDPRYNVPYEIENGVVWIPFRASDYSLKVVRMKIRPLETL

Target ORF information:

RefSeq Version	NM_001271911.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus fibrinogen alpha chain (FGA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271911.1

ORF Insert Sequence:

ATGATACCAG TGACGATACT CTGTGTGTTG CTCTGCCTCA ATTTAGCTTG GGCTCAGGAT 
GGGAAGACCA CCTTTGAAAA GGAGGGCGGA GGCGGGCGTG GCCCCAGGAT CCTAGAGAAC 
ATGCACGAGT CGTCCTGCAA GTATGAGAAG AACTGGCCTA TCTGTGTGGA TGATGACTGG 
GGTACAAAAT GTCCATCAGG CTGCAGAATG CAAGGAATAA TTGACGACAC AGACCAGAAT 
TACAGTCAAA GAATAGACAA TATTAGGCAG CAACTCGCAG ACAGTCAAAA CAAGTATAAA 
ACATCCAATC GGGTAATTGT GGAAACCATA AATATACTAA AGCCTGGTCT GGAGGGTGCC 
CAGCAGCTCG ATGAGAATTA CGGTCATGTG TCAACAGAAC TGAGGAGGAG AATTGTGACA 
TTAAAGCAGC GAGTTGCCAC TCAAGTGAAC AGAATTAAAG CTCTGCAGAA CAGCATCCAG 
GAGCAGGTGG TGGAAATGAA GCGCTTGGAG GTGGACATTG ATATTAAGAT ACGAGCTTGC 
AAAGGGAGCT GTGCTAGAAG TTTTGATTAC CAGGTGGACA AAGAAGGCTA TGACAACATC 
CAGAAGCATC TTACCCAGGC CAGCTCCATT GATATGCACC CAGACTTTCA AACAACCACC 
TTGAGCACAC TGAAAATGAG GCCTCTTAAG GACTCGAATG TTCCTGAACA TTTTAAGCTC 
AAGCCTTCAC CAGAAATGCA AGCTATGAGT GCATTTAATA ACATCAAACA GATGCAAGTG 
GTATTAGAAA GACCAGAAAC AGACCACGTG GCAGAAGCAA GGGGGGATTC TTCACCTTCC 
CACACTGGCA AATTAATTAC TTCTTCTCAC AGGAGAGAGA GCCCTAGCCT GGTAGACAAA 
ACCTCCTCTG CCTCCTCTGT CCATAGATGC ACCAGAACTG TCACCAAGAA AGTCATTAGT 
GGCCCTGATG GTCCTAGAGA AGAAATAGTT GAAAAAATGG TTTCCTCTGA TGGCTCAGAC 
TGCTCCCATC TACAAGGAGG ACGAGAAGGA AGCACGTACC ATTTTAGTGG GACAGGTGAC 
TTTCACAAGC TAGACAGGCT TCTGCCAGAT CTAGAGTCAT TCTTCACCCA TGACTCTGTA 
TCCACTAGTA GTAGGCACTC AATTGGCTCA AGCACCAGCA GCCATGTGAC TGGGGCAGGC 
AGCAGCCACC TAGGCACTGG AGGGAAAGAC AAATTCACAG ATTTAGGGGA GGAGGAAGAA 
GATGACTTTG GAGGACTTCA GCCATCTGGA TTTGCTGCTG GCAGTGCGAG TCACTCCAAG 
ACTGTACTGA CCAGCTCCTC TTCTAGTTTC AACAAGGGAG GCTCCACTTT TGAAACCAAG 
TCACTAAAGA CCCGTGAAAC ATCTGAGCAG CTAGGTGGGG TGCAACATGA TCAGAGTGCA 
GAGGACACCC CAGACTTTAA GGCGCGCAGC TTCAGACCAG CAGCAATGAG TACAAGGAGA 
TCCTACAATG GGAAAGACTG CGATGATATC CGCCAGAAAC ACACTTCTGG TGCCAAAAGT 
GGCATTTTCA AAATCAAGCC AGAGGGATCC AATAAGGTTT TGTCAGTTTA TTGCGACCAA 
GAGACCACTT TGGGAGGATG GCTATTGATC CAACAGAGAA TGGATGGATC AGTGAATTTT 
AACCGTACGT GGCAAGACTA CAGGAGAGGT TTCGGCAGCG TGGATGGCAA AGGGCAAGGA 
GAGTTGTGGC TGGGCAATGA GAATATCCAC TTGCTGACTC AGAACGATAC TCTGCTTCGA 
GTAGAGTTAG AGGACTGGGA TGGAAATGCT GCATATGCAG AGTACATCGT GCAGGTAGGG 
ACTGAAGCAG AAGGCTATGC CCTGACTGTG TCCTCCTATG AGGGGACTGC TGGGGATGCT 
CTGGTTGCTG GCTGGCTGGA GGAGGGCTCT GAGTACACAT CCCATGCCCA GATGCAGTTC 
AGCACTTTTG ACCGGGACCA GGACCACTGG GAGGAGAGCT GTGCAGAGGT GTACGGGGGT 
GGCTGGTGGT ACAACAGCTG CCAGGCAGCC AACCTCAATG GCATTTACTA TCCAGGGGGC 
CATTATGACC CCAGGTACAA TGTTCCATAT GAGATCGAGA ATGGAGTAGT CTGGATACCA 
TTTAGAGCCT CCGATTATTC CCTCAAAGTT GTTAGAATGA AAATCAGACC CCTGGAAACC 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FGA ( NM_205356.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990687.2 Gallus gallus fibrinogen alpha chain (FGA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OGa00158
Clone ID Related Accession (Same CDS sequence)	NM_205356.2
Accession Version	NM_205356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577030400000
Organism	Gallus gallus(chicken)
Product	fibrinogen alpha chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG641954.1, M34096.1, AADN05000019.1 and BU125048.1. On Dec 12, 2012 this sequence version replaced NM_205356.1. Transcript Variant: This variant (2) differs in the 3' UTR, and uses an alternate exon structure in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to variant 1. Sequence Note: This RefSeq record was created from transcripts of different breeds and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BG641954.1, M34096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201370, SAMEA2201378 [ECO:0000348] ##Evidence-Data-END##

ATGATACCAG TGACGATACT CTGTGTGTTG CTCTGCCTCA ATTTAGCTTG GGCTCAGGAT 
GGGAAGACCA CCTTTGAAAA GGAGGGCGGA GGCGGGCGTG GCCCCAGGAT CCTAGAGAAC 
ATGCACGAGT CGTCCTGCAA GTATGAGAAG AACTGGCCTA TCTGTGTGGA TGATGACTGG 
GGTACAAAAT GTCCATCAGG CTGCAGAATG CAAGGAATAA TTGACGACAC AGACCAGAAT 
TACAGTCAAA GAATAGACAA TATTAGGCAG CAACTCGCAG ACAGTCAAAA CAAGTATAAA 
ACATCCAATC GGGTAATTGT GGAAACCATA AATATACTAA AGCCTGGTCT GGAGGGTGCC 
CAGCAGCTCG ATGAGAATTA CGGTCATGTG TCAACAGAAC TGAGGAGGAG AATTGTGACA 
TTAAAGCAGC GAGTTGCCAC TCAAGTGAAC AGAATTAAAG CTCTGCAGAA CAGCATCCAG 
GAGCAGGTGG TGGAAATGAA GCGCTTGGAG GTGGACATTG ATATTAAGAT ACGAGCTTGC 
AAAGGGAGCT GTGCTAGAAG TTTTGATTAC CAGGTGGACA AAGAAGGCTA TGACAACATC 
CAGAAGCATC TTACCCAGGC CAGCTCCATT GATATGCACC CAGACTTTCA AACAACCACC 
TTGAGCACAC TGAAAATGAG GCCTCTTAAG GACTCGAATG TTCCTGAACA TTTTAAGCTC 
AAGCCTTCAC CAGAAATGCA AGCTATGAGT GCATTTAATA ACATCAAACA GATGCAAGTG 
GTATTAGAAA GACCAGAAAC AGACCACGTG GCAGAAGCAA GGGGGGATTC TTCACCTTCC 
CACACTGGCA AATTAATTAC TTCTTCTCAC AGGAGAGAGA GCCCTAGCCT GGTAGACAAA 
ACCTCCTCTG CCTCCTCTGT CCATAGATGC ACCAGAACTG TCACCAAGAA AGTCATTAGT 
GGCCCTGATG GTCCTAGAGA AGAAATAGTT GAAAAAATGG TTTCCTCTGA TGGCTCAGAC 
TGCTCCCATC TACAAGGAGG ACGAGAAGGA AGCACGTACC ATTTTAGTGG GACAGGTGAC 
TTTCACAAGC TAGACAGGCT TCTGCCAGAT CTAGAGTCAT TCTTCACCCA TGACTCTGTA 
TCCACTAGTA GTAGGCACTC AATTGGCTCA AGCACCAGCA GCCATGTGAC TGGGGCAGGC 
AGCAGCCACC TAGGCACTGG AGGGAAAGAC AAATTCACAG ATTTAGGGGA GGAGGAAGAA 
GATGACTTTG GAGGACTTCA GCCATCTGGA TTTGCTGCTG GCAGTGCGAG TCACTCCAAG 
ACTGTACTGA CCAGCTCCTC TTCTAGTTTC AACAAGGGAG GCTCCACTTT TGAAACCAAG 
TCACTAAAGA CCCGTGAAAC ATCTGAGCAG CTAGGTGGGG TGCAACATGA TCAGAGTGCA 
GAGGACACCC CAGACTTTAA GGCGCGCAGC TTCAGACCAG CAGCAATGAG TACAAGGAGA 
TCCTACAATG GGAAAGGTAC ACAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990687.2
CDS	28..1557
Translation	MIPVTILCVLLCLNLAWAQDGKTTFEKEGGGGRGPRILENMHESSCKYEKNWPICVDDDWGTKCPSGCRMQGIIDDTDQNYSQRIDNIRQQLADSQNKYKTSNRVIVETINILKPGLEGAQQLDENYGHVSTELRRRIVTLKQRVATQVNRIKALQNSIQEQVVEMKRLEVDIDIKIRACKGSCARSFDYQVDKEGYDNIQKHLTQASSIDMHPDFQTTTLSTLKMRPLKDSNVPEHFKLKPSPEMQAMSAFNNIKQMQVVLERPETDHVAEARGDSSPSHTGKLITSSHRRESPSLVDKTSSASSVHRCTRTVTKKVISGPDGPREEIVEKMVSSDGSDCSHLQGGREGSTYHFSGTGDFHKLDRLLPDLESFFTHDSVSTSSRHSIGSSTSSHVTGAGSSHLGTGGKDKFTDLGEEEEDDFGGLQPSGFAAGSASHSKTVLTSSSSSFNKGGSTFETKSLKTRETSEQLGGVQHDQSAEDTPDFKARSFRPAAMSTRRSYNGKGTQK

Target ORF information:

RefSeq Version	NM_205356.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus fibrinogen alpha chain (FGA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205356.2

ORF Insert Sequence:

ATGATACCAG TGACGATACT CTGTGTGTTG CTCTGCCTCA ATTTAGCTTG GGCTCAGGAT 
GGGAAGACCA CCTTTGAAAA GGAGGGCGGA GGCGGGCGTG GCCCCAGGAT CCTAGAGAAC 
ATGCACGAGT CGTCCTGCAA GTATGAGAAG AACTGGCCTA TCTGTGTGGA TGATGACTGG 
GGTACAAAAT GTCCATCAGG CTGCAGAATG CAAGGAATAA TTGACGACAC AGACCAGAAT 
TACAGTCAAA GAATAGACAA TATTAGGCAG CAACTCGCAG ACAGTCAAAA CAAGTATAAA 
ACATCCAATC GGGTAATTGT GGAAACCATA AATATACTAA AGCCTGGTCT GGAGGGTGCC 
CAGCAGCTCG ATGAGAATTA CGGTCATGTG TCAACAGAAC TGAGGAGGAG AATTGTGACA 
TTAAAGCAGC GAGTTGCCAC TCAAGTGAAC AGAATTAAAG CTCTGCAGAA CAGCATCCAG 
GAGCAGGTGG TGGAAATGAA GCGCTTGGAG GTGGACATTG ATATTAAGAT ACGAGCTTGC 
AAAGGGAGCT GTGCTAGAAG TTTTGATTAC CAGGTGGACA AAGAAGGCTA TGACAACATC 
CAGAAGCATC TTACCCAGGC CAGCTCCATT GATATGCACC CAGACTTTCA AACAACCACC 
TTGAGCACAC TGAAAATGAG GCCTCTTAAG GACTCGAATG TTCCTGAACA TTTTAAGCTC 
AAGCCTTCAC CAGAAATGCA AGCTATGAGT GCATTTAATA ACATCAAACA GATGCAAGTG 
GTATTAGAAA GACCAGAAAC AGACCACGTG GCAGAAGCAA GGGGGGATTC TTCACCTTCC 
CACACTGGCA AATTAATTAC TTCTTCTCAC AGGAGAGAGA GCCCTAGCCT GGTAGACAAA 
ACCTCCTCTG CCTCCTCTGT CCATAGATGC ACCAGAACTG TCACCAAGAA AGTCATTAGT 
GGCCCTGATG GTCCTAGAGA AGAAATAGTT GAAAAAATGG TTTCCTCTGA TGGCTCAGAC 
TGCTCCCATC TACAAGGAGG ACGAGAAGGA AGCACGTACC ATTTTAGTGG GACAGGTGAC 
TTTCACAAGC TAGACAGGCT TCTGCCAGAT CTAGAGTCAT TCTTCACCCA TGACTCTGTA 
TCCACTAGTA GTAGGCACTC AATTGGCTCA AGCACCAGCA GCCATGTGAC TGGGGCAGGC 
AGCAGCCACC TAGGCACTGG AGGGAAAGAC AAATTCACAG ATTTAGGGGA GGAGGAAGAA 
GATGACTTTG GAGGACTTCA GCCATCTGGA TTTGCTGCTG GCAGTGCGAG TCACTCCAAG 
ACTGTACTGA CCAGCTCCTC TTCTAGTTTC AACAAGGGAG GCTCCACTTT TGAAACCAAG 
TCACTAAAGA CCCGTGAAAC ATCTGAGCAG CTAGGTGGGG TGCAACATGA TCAGAGTGCA 
GAGGACACCC CAGACTTTAA GGCGCGCAGC TTCAGACCAG CAGCAATGAG TACAAGGAGA 
TCCTACAATG GGAAAGGTAC ACAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Substitution of the human αC region with the analogous chicken domain generates a fibrinogen with severely impaired lateral aggregation: fibrin monomers assemble into protofibrils but protofibrils do not assemble into fibers.
Biochemistry50(42)9066-75(2011 Oct)
Ping L,Huang L,Cardinali B,Profumo A,Gorkun OV,Lord ST

Chicken genomics resource: sequencing and annotation of 35,407 ESTs from single and multiple tissue cDNA libraries and CAP3 assembly of a chicken gene index.
Physiological genomics25(3)514-24(2006 May)
Carre W,Wang X,Porter TE,Nys Y,Tang J,Bernberg E,Morgan R,Burnside J,Aggrey SE,Simon J,Cogburn LA

A comprehensive collection of chicken cDNAs.
Current biology : CB12(22)1965-9(2002 Nov)
Boardman PE,Sanz-Ezquerro J,Overton IM,Burt DW,Bosch E,Fong WT,Tickle C,Brown WR,Wilson SA,Hubbard SJ

Crystal structure of native chicken fibrinogen at 2.7 A resolution.
Biochemistry40(42)12515-23(2001 Oct)
Yang Z,Kollman JM,Pandi L,Doolittle RF

Crystal structure of native chicken fibrinogen at 5.5-A resolution.
Proceedings of the National Academy of Sciences of the United States of America97(8)3907-12(2000 Apr)
Yang Z,Mochalkin I,Veerapandian L,Riley M,Doolittle RF

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FGA cDNA ORF clone, Gallus gallus(chicken)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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