Dvir\tim cDNA ORF clone, Drosophila virilis

Gene
Clones
gRNAs

Summary

Gene Symbol	Dvir\tim
Entrez Gene ID	6628265
Full Name	GJ17601 gene product from transcript GJ17601-RD
Synonyms	Dvir\GJ17601,GJ17601,dvir_GLEANR_2182,tim,vtim
Gene Type	protein-coding
Organism	Drosophila virilis

mRNA and Protein(s)

mRNA	Protein	Name
XM_015172979.1	XP_015028465.1	tim, isoform B
XM_015172980.1	XP_015028466.1	tim, isoform D
XM_002052509.2	XP_002052545.2	tim, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dvir\tim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dvir\tim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODk19846	XM_015172980.1 Latest version!	Drosophila virilis tim, transcript variant D (Dvir\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODk19846	XM_002052509.2 Latest version!	Drosophila virilis tim, transcript variant C (Dvir\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODk20273	XM_015172979.1 Latest version!	Drosophila virilis tim, transcript variant B (Dvir\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dvir\tim ( XM_015172980.1 ) cDNA ORF clone, Drosophila virilis -> XP_015028466.1 Drosophila virilis tim, transcript variant D (Dvir\tim), mRNA.

Price & Availability↑

CloneID	ODk19846
Clone ID Related Accession (Same CDS sequence)	XM_015172980.1 , XM_002052509.2
Accession Version	XM_015172980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila virilis
Product	tim, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002014421). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTCGCAAC ACCTCAGCTT CAGAGCGTCT TCTCCTCGTT GGGCTCCCTT 
GTTGGCGGCA CCTACGTGGT CAGTCCCAAT GCATTGGCAA TTTTGGAGGA GATTAACCAT 
AAACTTACCT ATGAGGATCA AACGCTGCGC ACTTTCCGTC GCGCCATCGG CTTTGGCCAG 
AATGTGCGAG TCGATCTAAT ACCATTGCTC GAGAATGCCA AGGATGATGC GGTGCTCGAG 
TCAGTCATAC GGATATTGGT TAATCTTACC GTGCCGGTGG AGTGCCTGTT CTCGGTGGAT 
CTCATGTACC GCACCGAGGT AGGGCGTCAC ACAATTTTCG AGCTGAACAA GCTGCTCTAC 
AACAGCAAGG AGGCCTTCAC CGATCCGAAG AGCACCAAGA GCGTCGTCGA GTATATGAAA 
CACATTCTGG AATCGGATCC AAAGCTATCG CCGCACAAAT GCGATCAGAT CAACAATTGT 
CTCCTGTTGT TGCGCAACAT TTTGCACATA CCGGAGACGC ATGCACATTT CCTGATGCCC 
AGGCTGCAGC CGGGCAGCGG CCATCAGGTG TCCATGCAGA ACACCATATT ATGGAACCTA 
TTCATACAGA GCATTGATAA GTTGCTCCTA TATCTGATGA CCTGTCCGCA GCGTTCGCTC 
TGGGGCGTGA CCATGGTGCA GCTGATTGCA CTGATCTACA AGGATCAGCA TGTGAGCACC 
TTGCAGAAGC TGCTGAACTT GTGGTTTGAG GCCTCACTGT CGGAGAGTTC GGATGACAAT 
GAGAGTAACA CAACGCCACC CAAACAGGCC AGCGGCGACT CCAGTCCCAT GCTCACCTCA 
GATCCCACAT CGGACTCTTC GGACAATGGC AGCGGCGGCA AGAAGGAAAG CTGCGAGGAG 
CGACGTCAGG CTCTGCGCGA GGGCACCGAT GCCACGCTAC ATGAGGTCAG TCGCAAAGGG 
CATGAATATC AGAATGCCAT GGCCAGCTCG AATGCGGCCA ACTATATACT CGAGGGACCC 
TGCTCGGCCC AACAGCCGTG GTCCGACTGT GAAATGCAGG AGTACAAACA GATGACTGCC 
GTCATCAGCG AACCATTAAA TCTTTCACAA CCAGCTGACA ATGTCAACTA CACTACCAAT 
GCGAATTACG CTCGCACCAC AAGCGCAGAT ATCTTGACCA AAACGACTAG CCTAAAACAT 
GAGGGCTTTA AGCCGCCCGC ACCACGTCGA AACACGCTCT CGGCCATATT GTCGGACAAT 
TACGCACCAC TCTCTTTTAT TTCGGCCGTC AAGCTGGGCC AGAAGTCACC GCACGCGGGT 
CAACTGCAGC TAATCAAGGG CAAATGCTGT CCGCAGAAGC GTGAGTGCCC ATCCTCACAG 
TCGGAGCACT CCGACTGCGG CTATGGCACC CAGATGGAGA ATCCAGAGTC CATATCCACA 
TCCAGCAATG ATGATGATGG TCCCCAGGGC AAGCCGCAAC ATCAGAAGCC GCCGTGCAGT 
AGTAAGCATC GCAGCAAACA ACGCATCTTT GCAGTGCCGC AGGATACAAA GGATCTGAGA 
CGCAAGAAGC TGGTCAAGCG TAGCAAGAGC AGCCTCATCA ACATGAAGGG CCTGGTGCTG 
CACACGCCCA ACGACGATGA CATATCCAAT TTGCTTAAAG AATTCACAGT CGACTTTTTA 
CTCAAAGGCT ACAACTATTT GGTAGAGGAG CTGCACTCCC AGCTGTTGTC CAATGCGAAG 
ATGCCCATTG ATACATCGCA TTTCTTCTGG CTTGTCACGT TCTTTCTCAA GTTTGCCGCC 
CAGCTGGAGC TGGACATGGA ACACATCGAT ACAATACTCA CCTTCGATGT GCTGAGCTTT 
CTAACCTACG AGGGTGTCAG CCTGTGCGAG CAGCTAGAGC TCAATGCACG ACAGGAGGGT 
GCTGATCTGA GACCCTATTT GCGTCGCATG CATCTGGTGG TCACGGCCAT ACGTGAGTTC 
CTGCAGGCCA TCGAAGCCTA CAACAAGGTC ACCCATCTCA GCGAGGATGA TCGCTATCGT 
CTACGCCAGC TCCAGCTACA GATAAGCGCT ACCACAGATC TGCGCTGCTT GTTTGTTCTG 
TTGCTGCGAC GATTTAATCC CAGCATCCAT TCCAAGCAGT ATTTACAAGA TCTGGTCGTG 
ACTAATCACA TTCTTTTGCT GATTTTGGAC AATGCTGCCA AATTGGAAGG CGGCCAGACC 
ATTGGACTCT CGGAGCACAT CTCACAATTT GCCACCTTGG AGGTGATGCA CTACTATGGC 
ATTCTCTTGG AGGACTTCAG CAACAATGGC GAATATGTCA ACGACTGCAT CTTTACCATG 
ATGCATCACA TTGGCGGCGA TCTGGACCAG GTTGGCGTTC TCTATCAGCC TATTATATTG 
AAGGCCTACT CTCGCATTTG GGAGGCCGAC TATGATATAT GCGATGACTG GAGCGATCTA 
ATTGAGTATG TTATTCACAA GTTTCTGAAC ACGCCACCCA AATCACCGAT GGCTATACCA 
ACCGCTTCGT TGACCGAGCT GACTAAGGAG CATAATCAGG AACACACTGC TTGTCCCTGG 
TCACAGGAGG ACATGGACTC GCTCTGTTGG TATTTTGTAC AGAGCAAGAG GCAAAACGAT 
GTGATTGGCA ATATTGCGAA GCTGTTTAGC AACAATGGCA ACAAGATAAA GACACGTATT 
TCTATCATTC AACAGCTGCT GCAACAGGAT ATCATTACGT TGCTGGAGTA CGATGATCTG 
ATGAAGTTCG AGGATGCCGA ATATCAGAGA ACTCTCCTGG CCACGCCGAC ATCGCTGACC 
ACCGATTCGG GCATTGAGTT GAAAGAAAGC GCCTATGGCA AACCCTCGGA TGATGTGCAG 
GTTCTGCTGG ATCTAATTAG AAAGGAGAAT AAGTCGCAGC ATTTGGTGTG GCTACAGAAA 
TTGTTGCTCG AGTGCTGCTA CGTCAAGATG ATGATCAAGT GCGGCAGCTG CCAGACGGAT 
GTGGAACCCA TCATGGAGCC GGTGGTCTAT CATTGCATGT TCAAGCAAAA GCCCATACCG 
GTGGTCCAGT GGAACAATGA ACAGTCGTCG ACGATGCTCT ATCAGCCCTT CATGCTGCTG 
CTGCACAAGC TTGGCATACA GCTGCCCGCG GATGCGGGCT TAATATTTGC GAGAATACCA 
GATTTTTGGA CTCCGGAAAC GATGTATGGG CTGGCAAAGA AGCTGGGACC GTTGGATAAA 
CTGACGCTCA AGTTTGATCC TCGCGATCTG GAGGATGCTC CCCCGTCGCG CCATCACACC 
GGCGCCCGCA ACTCGCTCAG CTCGATTAGC AGCCTGGAGG CTGATTTCGG CGATTCTGAG 
GGCCTGGCAC TCATACCCGA AGTGGATCCA GCTGTGGAGA AGGCAGCAGC AGCGGCTTCT 
GCTATACCAA ACAATGAAAT ATTTGCCTTG CCCAGGGTAA AGCACTGCAA CTCCATCATC 
AGATACACGC CGGATCCGAC ACCGCCCGTG CCCAGTTGGC TGCAGTTGGT CATGCGCAGC 
AAATGCAGCA AGCGACGCAG TCCAGCCACA GACGCCAGCG ATTGCACCAG TACCAGCACA 
ATGATTGCCG ATGACGAGAT CAAGTCATAT GCCTCCATGG CTCAGGCTTC ACATACAAAG 
CTGAACGCCG GCTATCCGAC TAGTACGTTG GTCTGTATGA ACAAGTTGAG CAACTGCAGC 
TTCGCGGCGC CACCGAATGA GAACAGCAGC AGCTCCGGCT GTGGCGGCAC TGCATCTTCG 
ATGTCAATGC CAAATATGCC GGATGGAAAT TCGGATGCGT TGATGAAGAC GTCGTTTGAG 
CGGCTGGCGG TGACGGGCGC ACGCTACTTG CGGCCCAGCA ATACGGATCA GGATTACAGC 
GCCTTGGTGG CATCGGTCTA CGAAAACGAG TTTGCCAACA GCGACAATGT GTCAGTGGCC 
TCGGACTTGA CCCGCATGTA TGTCAGCGAT GAGGATGAGA AACATGAGCT CCAATTACAA 
CAGGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015028466.1
CDS	1121..5152
Translation	MDWLLATPQLQSVFSSLGSLVGGTYVVSPNALAILEEINHKLTYEDQTLRTFRRAIGFGQNVRVDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDLMYRTEVGRHTIFELNKLLYNSKEAFTDPKSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHFLMPRLQPGSGHQVSMQNTILWNLFIQSIDKLLLYLMTCPQRSLWGVTMVQLIALIYKDQHVSTLQKLLNLWFEASLSESSDDNESNTTPPKQASGDSSPMLTSDPTSDSSDNGSGGKKESCEERRQALREGTDATLHEVSRKGHEYQNAMASSNAANYILEGPCSAQQPWSDCEMQEYKQMTAVISEPLNLSQPADNVNYTTNANYARTTSADILTKTTSLKHEGFKPPAPRRNTLSAILSDNYAPLSFISAVKLGQKSPHAGQLQLIKGKCCPQKRECPSSQSEHSDCGYGTQMENPESISTSSNDDDGPQGKPQHQKPPCSSKHRSKQRIFAVPQDTKDLRRKKLVKRSKSSLINMKGLVLHTPNDDDISNLLKEFTVDFLLKGYNYLVEELHSQLLSNAKMPIDTSHFFWLVTFFLKFAAQLELDMEHIDTILTFDVLSFLTYEGVSLCEQLELNARQEGADLRPYLRRMHLVVTAIREFLQAIEAYNKVTHLSEDDRYRLRQLQLQISATTDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDNAAKLEGGQTIGLSEHISQFATLEVMHYYGILLEDFSNNGEYVNDCIFTMMHHIGGDLDQVGVLYQPIILKAYSRIWEADYDICDDWSDLIEYVIHKFLNTPPKSPMAIPTASLTELTKEHNQEHTACPWSQEDMDSLCWYFVQSKRQNDVIGNIAKLFSNNGNKIKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLATPTSLTTDSGIELKESAYGKPSDDVQVLLDLIRKENKSQHLVWLQKLLLECCYVKMMIKCGSCQTDVEPIMEPVVYHCMFKQKPIPVVQWNNEQSSTMLYQPFMLLLHKLGIQLPADAGLIFARIPDFWTPETMYGLAKKLGPLDKLTLKFDPRDLEDAPPSRHHTGARNSLSSISSLEADFGDSEGLALIPEVDPAVEKAAAAASAIPNNEIFALPRVKHCNSIIRYTPDPTPPVPSWLQLVMRSKCSKRRSPATDASDCTSTSTMIADDEIKSYASMAQASHTKLNAGYPTSTLVCMNKLSNCSFAAPPNENSSSSGCGGTASSMSMPNMPDGNSDALMKTSFERLAVTGARYLRPSNTDQDYSALVASVYENEFANSDNVSVASDLTRMYVSDEDEKHELQLQQVE

Target ORF information:

RefSeq Version	XM_015172980.1
Organism	Drosophila virilis
Definition	Drosophila virilis tim, transcript variant D (Dvir\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015172980.1

ORF Insert Sequence:

ATGGACTGGT TACTCGCAAC ACCTCAGCTT CAGAGCGTCT TCTCCTCGTT GGGCTCCCTT 
GTTGGCGGCA CCTACGTGGT CAGTCCCAAT GCATTGGCAA TTTTGGAGGA GATTAACCAT 
AAACTTACCT ATGAGGATCA AACGCTGCGC ACTTTCCGTC GCGCCATCGG CTTTGGCCAG 
AATGTGCGAG TCGATCTAAT ACCATTGCTC GAGAATGCCA AGGATGATGC GGTGCTCGAG 
TCAGTCATAC GGATATTGGT TAATCTTACC GTGCCGGTGG AGTGCCTGTT CTCGGTGGAT 
CTCATGTACC GCACCGAGGT AGGGCGTCAC ACAATTTTCG AGCTGAACAA GCTGCTCTAC 
AACAGCAAGG AGGCCTTCAC CGATCCGAAG AGCACCAAGA GCGTCGTCGA GTATATGAAA 
CACATTCTGG AATCGGATCC AAAGCTATCG CCGCACAAAT GCGATCAGAT CAACAATTGT 
CTCCTGTTGT TGCGCAACAT TTTGCACATA CCGGAGACGC ATGCACATTT CCTGATGCCC 
AGGCTGCAGC CGGGCAGCGG CCATCAGGTG TCCATGCAGA ACACCATATT ATGGAACCTA 
TTCATACAGA GCATTGATAA GTTGCTCCTA TATCTGATGA CCTGTCCGCA GCGTTCGCTC 
TGGGGCGTGA CCATGGTGCA GCTGATTGCA CTGATCTACA AGGATCAGCA TGTGAGCACC 
TTGCAGAAGC TGCTGAACTT GTGGTTTGAG GCCTCACTGT CGGAGAGTTC GGATGACAAT 
GAGAGTAACA CAACGCCACC CAAACAGGCC AGCGGCGACT CCAGTCCCAT GCTCACCTCA 
GATCCCACAT CGGACTCTTC GGACAATGGC AGCGGCGGCA AGAAGGAAAG CTGCGAGGAG 
CGACGTCAGG CTCTGCGCGA GGGCACCGAT GCCACGCTAC ATGAGGTCAG TCGCAAAGGG 
CATGAATATC AGAATGCCAT GGCCAGCTCG AATGCGGCCA ACTATATACT CGAGGGACCC 
TGCTCGGCCC AACAGCCGTG GTCCGACTGT GAAATGCAGG AGTACAAACA GATGACTGCC 
GTCATCAGCG AACCATTAAA TCTTTCACAA CCAGCTGACA ATGTCAACTA CACTACCAAT 
GCGAATTACG CTCGCACCAC AAGCGCAGAT ATCTTGACCA AAACGACTAG CCTAAAACAT 
GAGGGCTTTA AGCCGCCCGC ACCACGTCGA AACACGCTCT CGGCCATATT GTCGGACAAT 
TACGCACCAC TCTCTTTTAT TTCGGCCGTC AAGCTGGGCC AGAAGTCACC GCACGCGGGT 
CAACTGCAGC TAATCAAGGG CAAATGCTGT CCGCAGAAGC GTGAGTGCCC ATCCTCACAG 
TCGGAGCACT CCGACTGCGG CTATGGCACC CAGATGGAGA ATCCAGAGTC CATATCCACA 
TCCAGCAATG ATGATGATGG TCCCCAGGGC AAGCCGCAAC ATCAGAAGCC GCCGTGCAGT 
AGTAAGCATC GCAGCAAACA ACGCATCTTT GCAGTGCCGC AGGATACAAA GGATCTGAGA 
CGCAAGAAGC TGGTCAAGCG TAGCAAGAGC AGCCTCATCA ACATGAAGGG CCTGGTGCTG 
CACACGCCCA ACGACGATGA CATATCCAAT TTGCTTAAAG AATTCACAGT CGACTTTTTA 
CTCAAAGGCT ACAACTATTT GGTAGAGGAG CTGCACTCCC AGCTGTTGTC CAATGCGAAG 
ATGCCCATTG ATACATCGCA TTTCTTCTGG CTTGTCACGT TCTTTCTCAA GTTTGCCGCC 
CAGCTGGAGC TGGACATGGA ACACATCGAT ACAATACTCA CCTTCGATGT GCTGAGCTTT 
CTAACCTACG AGGGTGTCAG CCTGTGCGAG CAGCTAGAGC TCAATGCACG ACAGGAGGGT 
GCTGATCTGA GACCCTATTT GCGTCGCATG CATCTGGTGG TCACGGCCAT ACGTGAGTTC 
CTGCAGGCCA TCGAAGCCTA CAACAAGGTC ACCCATCTCA GCGAGGATGA TCGCTATCGT 
CTACGCCAGC TCCAGCTACA GATAAGCGCT ACCACAGATC TGCGCTGCTT GTTTGTTCTG 
TTGCTGCGAC GATTTAATCC CAGCATCCAT TCCAAGCAGT ATTTACAAGA TCTGGTCGTG 
ACTAATCACA TTCTTTTGCT GATTTTGGAC AATGCTGCCA AATTGGAAGG CGGCCAGACC 
ATTGGACTCT CGGAGCACAT CTCACAATTT GCCACCTTGG AGGTGATGCA CTACTATGGC 
ATTCTCTTGG AGGACTTCAG CAACAATGGC GAATATGTCA ACGACTGCAT CTTTACCATG 
ATGCATCACA TTGGCGGCGA TCTGGACCAG GTTGGCGTTC TCTATCAGCC TATTATATTG 
AAGGCCTACT CTCGCATTTG GGAGGCCGAC TATGATATAT GCGATGACTG GAGCGATCTA 
ATTGAGTATG TTATTCACAA GTTTCTGAAC ACGCCACCCA AATCACCGAT GGCTATACCA 
ACCGCTTCGT TGACCGAGCT GACTAAGGAG CATAATCAGG AACACACTGC TTGTCCCTGG 
TCACAGGAGG ACATGGACTC GCTCTGTTGG TATTTTGTAC AGAGCAAGAG GCAAAACGAT 
GTGATTGGCA ATATTGCGAA GCTGTTTAGC AACAATGGCA ACAAGATAAA GACACGTATT 
TCTATCATTC AACAGCTGCT GCAACAGGAT ATCATTACGT TGCTGGAGTA CGATGATCTG 
ATGAAGTTCG AGGATGCCGA ATATCAGAGA ACTCTCCTGG CCACGCCGAC ATCGCTGACC 
ACCGATTCGG GCATTGAGTT GAAAGAAAGC GCCTATGGCA AACCCTCGGA TGATGTGCAG 
GTTCTGCTGG ATCTAATTAG AAAGGAGAAT AAGTCGCAGC ATTTGGTGTG GCTACAGAAA 
TTGTTGCTCG AGTGCTGCTA CGTCAAGATG ATGATCAAGT GCGGCAGCTG CCAGACGGAT 
GTGGAACCCA TCATGGAGCC GGTGGTCTAT CATTGCATGT TCAAGCAAAA GCCCATACCG 
GTGGTCCAGT GGAACAATGA ACAGTCGTCG ACGATGCTCT ATCAGCCCTT CATGCTGCTG 
CTGCACAAGC TTGGCATACA GCTGCCCGCG GATGCGGGCT TAATATTTGC GAGAATACCA 
GATTTTTGGA CTCCGGAAAC GATGTATGGG CTGGCAAAGA AGCTGGGACC GTTGGATAAA 
CTGACGCTCA AGTTTGATCC TCGCGATCTG GAGGATGCTC CCCCGTCGCG CCATCACACC 
GGCGCCCGCA ACTCGCTCAG CTCGATTAGC AGCCTGGAGG CTGATTTCGG CGATTCTGAG 
GGCCTGGCAC TCATACCCGA AGTGGATCCA GCTGTGGAGA AGGCAGCAGC AGCGGCTTCT 
GCTATACCAA ACAATGAAAT ATTTGCCTTG CCCAGGGTAA AGCACTGCAA CTCCATCATC 
AGATACACGC CGGATCCGAC ACCGCCCGTG CCCAGTTGGC TGCAGTTGGT CATGCGCAGC 
AAATGCAGCA AGCGACGCAG TCCAGCCACA GACGCCAGCG ATTGCACCAG TACCAGCACA 
ATGATTGCCG ATGACGAGAT CAAGTCATAT GCCTCCATGG CTCAGGCTTC ACATACAAAG 
CTGAACGCCG GCTATCCGAC TAGTACGTTG GTCTGTATGA ACAAGTTGAG CAACTGCAGC 
TTCGCGGCGC CACCGAATGA GAACAGCAGC AGCTCCGGCT GTGGCGGCAC TGCATCTTCG 
ATGTCAATGC CAAATATGCC GGATGGAAAT TCGGATGCGT TGATGAAGAC GTCGTTTGAG 
CGGCTGGCGG TGACGGGCGC ACGCTACTTG CGGCCCAGCA ATACGGATCA GGATTACAGC 
GCCTTGGTGG CATCGGTCTA CGAAAACGAG TTTGCCAACA GCGACAATGT GTCAGTGGCC 
TCGGACTTGA CCCGCATGTA TGTCAGCGAT GAGGATGAGA AACATGAGCT CCAATTACAA 
CAGGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dvir\tim ( XM_002052509.2 ) cDNA ORF clone, Drosophila virilis -> XP_002052545.2 Drosophila virilis tim, transcript variant C (Dvir\tim), mRNA.

Price & Availability↑

CloneID	ODk19846
Clone ID Related Accession (Same CDS sequence)	XM_015172980.1 , XM_002052509.2
Accession Version	XM_002052509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila virilis
Product	tim, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002014421). On Dec 21, 2015 this sequence version replaced XM_002052509.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTCGCAAC ACCTCAGCTT CAGAGCGTCT TCTCCTCGTT GGGCTCCCTT 
GTTGGCGGCA CCTACGTGGT CAGTCCCAAT GCATTGGCAA TTTTGGAGGA GATTAACCAT 
AAACTTACCT ATGAGGATCA AACGCTGCGC ACTTTCCGTC GCGCCATCGG CTTTGGCCAG 
AATGTGCGAG TCGATCTAAT ACCATTGCTC GAGAATGCCA AGGATGATGC GGTGCTCGAG 
TCAGTCATAC GGATATTGGT TAATCTTACC GTGCCGGTGG AGTGCCTGTT CTCGGTGGAT 
CTCATGTACC GCACCGAGGT AGGGCGTCAC ACAATTTTCG AGCTGAACAA GCTGCTCTAC 
AACAGCAAGG AGGCCTTCAC CGATCCGAAG AGCACCAAGA GCGTCGTCGA GTATATGAAA 
CACATTCTGG AATCGGATCC AAAGCTATCG CCGCACAAAT GCGATCAGAT CAACAATTGT 
CTCCTGTTGT TGCGCAACAT TTTGCACATA CCGGAGACGC ATGCACATTT CCTGATGCCC 
AGGCTGCAGC CGGGCAGCGG CCATCAGGTG TCCATGCAGA ACACCATATT ATGGAACCTA 
TTCATACAGA GCATTGATAA GTTGCTCCTA TATCTGATGA CCTGTCCGCA GCGTTCGCTC 
TGGGGCGTGA CCATGGTGCA GCTGATTGCA CTGATCTACA AGGATCAGCA TGTGAGCACC 
TTGCAGAAGC TGCTGAACTT GTGGTTTGAG GCCTCACTGT CGGAGAGTTC GGATGACAAT 
GAGAGTAACA CAACGCCACC CAAACAGGCC AGCGGCGACT CCAGTCCCAT GCTCACCTCA 
GATCCCACAT CGGACTCTTC GGACAATGGC AGCGGCGGCA AGAAGGAAAG CTGCGAGGAG 
CGACGTCAGG CTCTGCGCGA GGGCACCGAT GCCACGCTAC ATGAGGTCAG TCGCAAAGGG 
CATGAATATC AGAATGCCAT GGCCAGCTCG AATGCGGCCA ACTATATACT CGAGGGACCC 
TGCTCGGCCC AACAGCCGTG GTCCGACTGT GAAATGCAGG AGTACAAACA GATGACTGCC 
GTCATCAGCG AACCATTAAA TCTTTCACAA CCAGCTGACA ATGTCAACTA CACTACCAAT 
GCGAATTACG CTCGCACCAC AAGCGCAGAT ATCTTGACCA AAACGACTAG CCTAAAACAT 
GAGGGCTTTA AGCCGCCCGC ACCACGTCGA AACACGCTCT CGGCCATATT GTCGGACAAT 
TACGCACCAC TCTCTTTTAT TTCGGCCGTC AAGCTGGGCC AGAAGTCACC GCACGCGGGT 
CAACTGCAGC TAATCAAGGG CAAATGCTGT CCGCAGAAGC GTGAGTGCCC ATCCTCACAG 
TCGGAGCACT CCGACTGCGG CTATGGCACC CAGATGGAGA ATCCAGAGTC CATATCCACA 
TCCAGCAATG ATGATGATGG TCCCCAGGGC AAGCCGCAAC ATCAGAAGCC GCCGTGCAGT 
AGTAAGCATC GCAGCAAACA ACGCATCTTT GCAGTGCCGC AGGATACAAA GGATCTGAGA 
CGCAAGAAGC TGGTCAAGCG TAGCAAGAGC AGCCTCATCA ACATGAAGGG CCTGGTGCTG 
CACACGCCCA ACGACGATGA CATATCCAAT TTGCTTAAAG AATTCACAGT CGACTTTTTA 
CTCAAAGGCT ACAACTATTT GGTAGAGGAG CTGCACTCCC AGCTGTTGTC CAATGCGAAG 
ATGCCCATTG ATACATCGCA TTTCTTCTGG CTTGTCACGT TCTTTCTCAA GTTTGCCGCC 
CAGCTGGAGC TGGACATGGA ACACATCGAT ACAATACTCA CCTTCGATGT GCTGAGCTTT 
CTAACCTACG AGGGTGTCAG CCTGTGCGAG CAGCTAGAGC TCAATGCACG ACAGGAGGGT 
GCTGATCTGA GACCCTATTT GCGTCGCATG CATCTGGTGG TCACGGCCAT ACGTGAGTTC 
CTGCAGGCCA TCGAAGCCTA CAACAAGGTC ACCCATCTCA GCGAGGATGA TCGCTATCGT 
CTACGCCAGC TCCAGCTACA GATAAGCGCT ACCACAGATC TGCGCTGCTT GTTTGTTCTG 
TTGCTGCGAC GATTTAATCC CAGCATCCAT TCCAAGCAGT ATTTACAAGA TCTGGTCGTG 
ACTAATCACA TTCTTTTGCT GATTTTGGAC AATGCTGCCA AATTGGAAGG CGGCCAGACC 
ATTGGACTCT CGGAGCACAT CTCACAATTT GCCACCTTGG AGGTGATGCA CTACTATGGC 
ATTCTCTTGG AGGACTTCAG CAACAATGGC GAATATGTCA ACGACTGCAT CTTTACCATG 
ATGCATCACA TTGGCGGCGA TCTGGACCAG GTTGGCGTTC TCTATCAGCC TATTATATTG 
AAGGCCTACT CTCGCATTTG GGAGGCCGAC TATGATATAT GCGATGACTG GAGCGATCTA 
ATTGAGTATG TTATTCACAA GTTTCTGAAC ACGCCACCCA AATCACCGAT GGCTATACCA 
ACCGCTTCGT TGACCGAGCT GACTAAGGAG CATAATCAGG AACACACTGC TTGTCCCTGG 
TCACAGGAGG ACATGGACTC GCTCTGTTGG TATTTTGTAC AGAGCAAGAG GCAAAACGAT 
GTGATTGGCA ATATTGCGAA GCTGTTTAGC AACAATGGCA ACAAGATAAA GACACGTATT 
TCTATCATTC AACAGCTGCT GCAACAGGAT ATCATTACGT TGCTGGAGTA CGATGATCTG 
ATGAAGTTCG AGGATGCCGA ATATCAGAGA ACTCTCCTGG CCACGCCGAC ATCGCTGACC 
ACCGATTCGG GCATTGAGTT GAAAGAAAGC GCCTATGGCA AACCCTCGGA TGATGTGCAG 
GTTCTGCTGG ATCTAATTAG AAAGGAGAAT AAGTCGCAGC ATTTGGTGTG GCTACAGAAA 
TTGTTGCTCG AGTGCTGCTA CGTCAAGATG ATGATCAAGT GCGGCAGCTG CCAGACGGAT 
GTGGAACCCA TCATGGAGCC GGTGGTCTAT CATTGCATGT TCAAGCAAAA GCCCATACCG 
GTGGTCCAGT GGAACAATGA ACAGTCGTCG ACGATGCTCT ATCAGCCCTT CATGCTGCTG 
CTGCACAAGC TTGGCATACA GCTGCCCGCG GATGCGGGCT TAATATTTGC GAGAATACCA 
GATTTTTGGA CTCCGGAAAC GATGTATGGG CTGGCAAAGA AGCTGGGACC GTTGGATAAA 
CTGACGCTCA AGTTTGATCC TCGCGATCTG GAGGATGCTC CCCCGTCGCG CCATCACACC 
GGCGCCCGCA ACTCGCTCAG CTCGATTAGC AGCCTGGAGG CTGATTTCGG CGATTCTGAG 
GGCCTGGCAC TCATACCCGA AGTGGATCCA GCTGTGGAGA AGGCAGCAGC AGCGGCTTCT 
GCTATACCAA ACAATGAAAT ATTTGCCTTG CCCAGGGTAA AGCACTGCAA CTCCATCATC 
AGATACACGC CGGATCCGAC ACCGCCCGTG CCCAGTTGGC TGCAGTTGGT CATGCGCAGC 
AAATGCAGCA AGCGACGCAG TCCAGCCACA GACGCCAGCG ATTGCACCAG TACCAGCACA 
ATGATTGCCG ATGACGAGAT CAAGTCATAT GCCTCCATGG CTCAGGCTTC ACATACAAAG 
CTGAACGCCG GCTATCCGAC TAGTACGTTG GTCTGTATGA ACAAGTTGAG CAACTGCAGC 
TTCGCGGCGC CACCGAATGA GAACAGCAGC AGCTCCGGCT GTGGCGGCAC TGCATCTTCG 
ATGTCAATGC CAAATATGCC GGATGGAAAT TCGGATGCGT TGATGAAGAC GTCGTTTGAG 
CGGCTGGCGG TGACGGGCGC ACGCTACTTG CGGCCCAGCA ATACGGATCA GGATTACAGC 
GCCTTGGTGG CATCGGTCTA CGAAAACGAG TTTGCCAACA GCGACAATGT GTCAGTGGCC 
TCGGACTTGA CCCGCATGTA TGTCAGCGAT GAGGATGAGA AACATGAGCT CCAATTACAA 
CAGGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002052545.2
CDS	981..5012
Translation	MDWLLATPQLQSVFSSLGSLVGGTYVVSPNALAILEEINHKLTYEDQTLRTFRRAIGFGQNVRVDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDLMYRTEVGRHTIFELNKLLYNSKEAFTDPKSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHFLMPRLQPGSGHQVSMQNTILWNLFIQSIDKLLLYLMTCPQRSLWGVTMVQLIALIYKDQHVSTLQKLLNLWFEASLSESSDDNESNTTPPKQASGDSSPMLTSDPTSDSSDNGSGGKKESCEERRQALREGTDATLHEVSRKGHEYQNAMASSNAANYILEGPCSAQQPWSDCEMQEYKQMTAVISEPLNLSQPADNVNYTTNANYARTTSADILTKTTSLKHEGFKPPAPRRNTLSAILSDNYAPLSFISAVKLGQKSPHAGQLQLIKGKCCPQKRECPSSQSEHSDCGYGTQMENPESISTSSNDDDGPQGKPQHQKPPCSSKHRSKQRIFAVPQDTKDLRRKKLVKRSKSSLINMKGLVLHTPNDDDISNLLKEFTVDFLLKGYNYLVEELHSQLLSNAKMPIDTSHFFWLVTFFLKFAAQLELDMEHIDTILTFDVLSFLTYEGVSLCEQLELNARQEGADLRPYLRRMHLVVTAIREFLQAIEAYNKVTHLSEDDRYRLRQLQLQISATTDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDNAAKLEGGQTIGLSEHISQFATLEVMHYYGILLEDFSNNGEYVNDCIFTMMHHIGGDLDQVGVLYQPIILKAYSRIWEADYDICDDWSDLIEYVIHKFLNTPPKSPMAIPTASLTELTKEHNQEHTACPWSQEDMDSLCWYFVQSKRQNDVIGNIAKLFSNNGNKIKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLATPTSLTTDSGIELKESAYGKPSDDVQVLLDLIRKENKSQHLVWLQKLLLECCYVKMMIKCGSCQTDVEPIMEPVVYHCMFKQKPIPVVQWNNEQSSTMLYQPFMLLLHKLGIQLPADAGLIFARIPDFWTPETMYGLAKKLGPLDKLTLKFDPRDLEDAPPSRHHTGARNSLSSISSLEADFGDSEGLALIPEVDPAVEKAAAAASAIPNNEIFALPRVKHCNSIIRYTPDPTPPVPSWLQLVMRSKCSKRRSPATDASDCTSTSTMIADDEIKSYASMAQASHTKLNAGYPTSTLVCMNKLSNCSFAAPPNENSSSSGCGGTASSMSMPNMPDGNSDALMKTSFERLAVTGARYLRPSNTDQDYSALVASVYENEFANSDNVSVASDLTRMYVSDEDEKHELQLQQVE

Target ORF information:

RefSeq Version	XM_002052509.2
Organism	Drosophila virilis
Definition	Drosophila virilis tim, transcript variant C (Dvir\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002052509.2

ORF Insert Sequence:

ATGGACTGGT TACTCGCAAC ACCTCAGCTT CAGAGCGTCT TCTCCTCGTT GGGCTCCCTT 
GTTGGCGGCA CCTACGTGGT CAGTCCCAAT GCATTGGCAA TTTTGGAGGA GATTAACCAT 
AAACTTACCT ATGAGGATCA AACGCTGCGC ACTTTCCGTC GCGCCATCGG CTTTGGCCAG 
AATGTGCGAG TCGATCTAAT ACCATTGCTC GAGAATGCCA AGGATGATGC GGTGCTCGAG 
TCAGTCATAC GGATATTGGT TAATCTTACC GTGCCGGTGG AGTGCCTGTT CTCGGTGGAT 
CTCATGTACC GCACCGAGGT AGGGCGTCAC ACAATTTTCG AGCTGAACAA GCTGCTCTAC 
AACAGCAAGG AGGCCTTCAC CGATCCGAAG AGCACCAAGA GCGTCGTCGA GTATATGAAA 
CACATTCTGG AATCGGATCC AAAGCTATCG CCGCACAAAT GCGATCAGAT CAACAATTGT 
CTCCTGTTGT TGCGCAACAT TTTGCACATA CCGGAGACGC ATGCACATTT CCTGATGCCC 
AGGCTGCAGC CGGGCAGCGG CCATCAGGTG TCCATGCAGA ACACCATATT ATGGAACCTA 
TTCATACAGA GCATTGATAA GTTGCTCCTA TATCTGATGA CCTGTCCGCA GCGTTCGCTC 
TGGGGCGTGA CCATGGTGCA GCTGATTGCA CTGATCTACA AGGATCAGCA TGTGAGCACC 
TTGCAGAAGC TGCTGAACTT GTGGTTTGAG GCCTCACTGT CGGAGAGTTC GGATGACAAT 
GAGAGTAACA CAACGCCACC CAAACAGGCC AGCGGCGACT CCAGTCCCAT GCTCACCTCA 
GATCCCACAT CGGACTCTTC GGACAATGGC AGCGGCGGCA AGAAGGAAAG CTGCGAGGAG 
CGACGTCAGG CTCTGCGCGA GGGCACCGAT GCCACGCTAC ATGAGGTCAG TCGCAAAGGG 
CATGAATATC AGAATGCCAT GGCCAGCTCG AATGCGGCCA ACTATATACT CGAGGGACCC 
TGCTCGGCCC AACAGCCGTG GTCCGACTGT GAAATGCAGG AGTACAAACA GATGACTGCC 
GTCATCAGCG AACCATTAAA TCTTTCACAA CCAGCTGACA ATGTCAACTA CACTACCAAT 
GCGAATTACG CTCGCACCAC AAGCGCAGAT ATCTTGACCA AAACGACTAG CCTAAAACAT 
GAGGGCTTTA AGCCGCCCGC ACCACGTCGA AACACGCTCT CGGCCATATT GTCGGACAAT 
TACGCACCAC TCTCTTTTAT TTCGGCCGTC AAGCTGGGCC AGAAGTCACC GCACGCGGGT 
CAACTGCAGC TAATCAAGGG CAAATGCTGT CCGCAGAAGC GTGAGTGCCC ATCCTCACAG 
TCGGAGCACT CCGACTGCGG CTATGGCACC CAGATGGAGA ATCCAGAGTC CATATCCACA 
TCCAGCAATG ATGATGATGG TCCCCAGGGC AAGCCGCAAC ATCAGAAGCC GCCGTGCAGT 
AGTAAGCATC GCAGCAAACA ACGCATCTTT GCAGTGCCGC AGGATACAAA GGATCTGAGA 
CGCAAGAAGC TGGTCAAGCG TAGCAAGAGC AGCCTCATCA ACATGAAGGG CCTGGTGCTG 
CACACGCCCA ACGACGATGA CATATCCAAT TTGCTTAAAG AATTCACAGT CGACTTTTTA 
CTCAAAGGCT ACAACTATTT GGTAGAGGAG CTGCACTCCC AGCTGTTGTC CAATGCGAAG 
ATGCCCATTG ATACATCGCA TTTCTTCTGG CTTGTCACGT TCTTTCTCAA GTTTGCCGCC 
CAGCTGGAGC TGGACATGGA ACACATCGAT ACAATACTCA CCTTCGATGT GCTGAGCTTT 
CTAACCTACG AGGGTGTCAG CCTGTGCGAG CAGCTAGAGC TCAATGCACG ACAGGAGGGT 
GCTGATCTGA GACCCTATTT GCGTCGCATG CATCTGGTGG TCACGGCCAT ACGTGAGTTC 
CTGCAGGCCA TCGAAGCCTA CAACAAGGTC ACCCATCTCA GCGAGGATGA TCGCTATCGT 
CTACGCCAGC TCCAGCTACA GATAAGCGCT ACCACAGATC TGCGCTGCTT GTTTGTTCTG 
TTGCTGCGAC GATTTAATCC CAGCATCCAT TCCAAGCAGT ATTTACAAGA TCTGGTCGTG 
ACTAATCACA TTCTTTTGCT GATTTTGGAC AATGCTGCCA AATTGGAAGG CGGCCAGACC 
ATTGGACTCT CGGAGCACAT CTCACAATTT GCCACCTTGG AGGTGATGCA CTACTATGGC 
ATTCTCTTGG AGGACTTCAG CAACAATGGC GAATATGTCA ACGACTGCAT CTTTACCATG 
ATGCATCACA TTGGCGGCGA TCTGGACCAG GTTGGCGTTC TCTATCAGCC TATTATATTG 
AAGGCCTACT CTCGCATTTG GGAGGCCGAC TATGATATAT GCGATGACTG GAGCGATCTA 
ATTGAGTATG TTATTCACAA GTTTCTGAAC ACGCCACCCA AATCACCGAT GGCTATACCA 
ACCGCTTCGT TGACCGAGCT GACTAAGGAG CATAATCAGG AACACACTGC TTGTCCCTGG 
TCACAGGAGG ACATGGACTC GCTCTGTTGG TATTTTGTAC AGAGCAAGAG GCAAAACGAT 
GTGATTGGCA ATATTGCGAA GCTGTTTAGC AACAATGGCA ACAAGATAAA GACACGTATT 
TCTATCATTC AACAGCTGCT GCAACAGGAT ATCATTACGT TGCTGGAGTA CGATGATCTG 
ATGAAGTTCG AGGATGCCGA ATATCAGAGA ACTCTCCTGG CCACGCCGAC ATCGCTGACC 
ACCGATTCGG GCATTGAGTT GAAAGAAAGC GCCTATGGCA AACCCTCGGA TGATGTGCAG 
GTTCTGCTGG ATCTAATTAG AAAGGAGAAT AAGTCGCAGC ATTTGGTGTG GCTACAGAAA 
TTGTTGCTCG AGTGCTGCTA CGTCAAGATG ATGATCAAGT GCGGCAGCTG CCAGACGGAT 
GTGGAACCCA TCATGGAGCC GGTGGTCTAT CATTGCATGT TCAAGCAAAA GCCCATACCG 
GTGGTCCAGT GGAACAATGA ACAGTCGTCG ACGATGCTCT ATCAGCCCTT CATGCTGCTG 
CTGCACAAGC TTGGCATACA GCTGCCCGCG GATGCGGGCT TAATATTTGC GAGAATACCA 
GATTTTTGGA CTCCGGAAAC GATGTATGGG CTGGCAAAGA AGCTGGGACC GTTGGATAAA 
CTGACGCTCA AGTTTGATCC TCGCGATCTG GAGGATGCTC CCCCGTCGCG CCATCACACC 
GGCGCCCGCA ACTCGCTCAG CTCGATTAGC AGCCTGGAGG CTGATTTCGG CGATTCTGAG 
GGCCTGGCAC TCATACCCGA AGTGGATCCA GCTGTGGAGA AGGCAGCAGC AGCGGCTTCT 
GCTATACCAA ACAATGAAAT ATTTGCCTTG CCCAGGGTAA AGCACTGCAA CTCCATCATC 
AGATACACGC CGGATCCGAC ACCGCCCGTG CCCAGTTGGC TGCAGTTGGT CATGCGCAGC 
AAATGCAGCA AGCGACGCAG TCCAGCCACA GACGCCAGCG ATTGCACCAG TACCAGCACA 
ATGATTGCCG ATGACGAGAT CAAGTCATAT GCCTCCATGG CTCAGGCTTC ACATACAAAG 
CTGAACGCCG GCTATCCGAC TAGTACGTTG GTCTGTATGA ACAAGTTGAG CAACTGCAGC 
TTCGCGGCGC CACCGAATGA GAACAGCAGC AGCTCCGGCT GTGGCGGCAC TGCATCTTCG 
ATGTCAATGC CAAATATGCC GGATGGAAAT TCGGATGCGT TGATGAAGAC GTCGTTTGAG 
CGGCTGGCGG TGACGGGCGC ACGCTACTTG CGGCCCAGCA ATACGGATCA GGATTACAGC 
GCCTTGGTGG CATCGGTCTA CGAAAACGAG TTTGCCAACA GCGACAATGT GTCAGTGGCC 
TCGGACTTGA CCCGCATGTA TGTCAGCGAT GAGGATGAGA AACATGAGCT CCAATTACAA 
CAGGTGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dvir\tim ( XM_015172979.1 ) cDNA ORF clone, Drosophila virilis -> XP_015028465.1 Drosophila virilis tim, transcript variant B (Dvir\tim), mRNA.

Price & Availability↑

CloneID	ODk20273
Clone ID Related Accession (Same CDS sequence)	XM_015172979.1
Accession Version	XM_015172979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila virilis
Product	tim, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002014421). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTCGCAAC ACCTCAGCTT CAGAGCGTCT TCTCCTCGTT GGGCTCCCTT 
GTTGGCGGCA CCTACGTGGT CAGTCCCAAT GCATTGGCAA TTTTGGAGGA GATTAACCAT 
AAACTTACCT ATGAGGATCA AACGCTGCGC ACTTTCCGTC GCGCCATCGG CTTTGGCCAG 
AATGTGCGAG TCGATCTAAT ACCATTGCTC GAGAATGCCA AGGATGATGC GGTGCTCGAG 
TCAGTCATAC GGATATTGGT TAATCTTACC GTGCCGGTGG AGTGCCTGTT CTCGGTGGAT 
CTCATGTACC GCACCGAGGT AGGGCGTCAC ACAATTTTCG AGCTGAACAA GCTGCTCTAC 
AACAGCAAGG AGGCCTTCAC CGATCCGAAG AGCACCAAGA GCGTCGTCGA GTATATGAAA 
CACATTCTGG AATCGGATCC AAAGCTATCG CCGCACAAAT GCGATCAGAT CAACAATTGT 
CTCCTGTTGT TGCGCAACAT TTTGCACATA CCGGAGACGC ATGCACATTT CCTGATGCCC 
AGGCTGCAGC CGGGCAGCGG CCATCAGGTG TCCATGCAGA ACACCATATT ATGGAACCTA 
TTCATACAGA GCATTGATAA GTTGCTCCTA TATCTGATGA CCTGTCCGCA GCGTTCGCTC 
TGGGGCGTGA CCATGGTGCA GCTGATTGCA CTGATCTACA AGGATCAGCA TGTGAGCACC 
TTGCAGAAGC TGCTGAACTT GTGGTTTGAG GCCTCACTGT CGGAGAGTTC GGATGACAAT 
GAGAGTAACA CAACGCCACC CAAACAGGCC AGCGGCGACT CCAGTCCCAT GCTCACCTCA 
GATCCCACAT CGGACTCTTC GGACAATGGC AGCGGCGGCA AGAAGGAAAG CTGCGAGGAG 
CGACGTCAGG CTCTGCGCGA GGGCACCGAT GCCACGCTAC ATGAGGTCAG TCGCAAAGGG 
CATGAATATC AGAATGCCAT GGCCAGCTCG AATGCGGCCA ACTATATACT CGAGGGACCC 
TGCTCGGCCC AACAGCCGTG GTCCGACTGT GAAATGCAGG AGTACAAACA GATGACTGCC 
GTCATCAGCG AACCATTAAA TCTTTCACAA CCAGCTGACA ATGTCAACTA CACTACCAAT 
GCGAATTACG CTCGCACCAC AAGCGCAGAT ATCTTGACCA AAACGACTAG CCTAAAACAT 
GAGGGCTTTA AGCCGCCCGC ACCACGTCGA AACACGCTCT CGGCCATATT GTCGGACAAT 
TACGCACCAC TCTCTTTTAT TTCGGCCGTC AAGCTGGGCC AGAAGTCACC GCACGCGGGT 
CAACTGCAGC TAATCAAGGG CAAATGCTGT CCGCAGAAGC GTGAGTGCCC ATCCTCACAG 
TCGGAGCACT CCGACTGCGG CTATGGCACC CAGATGGAGA ATCCAGAGTC CATATCCACA 
TCCAGCAATG ATGATGATGG TCCCCAGGGC AAGCCGCAAC ATCAGAAGCC GCCGTGCAGT 
AGTAAGCATC GCAGCAAACA ACGCATCTTT GCAGTGCCGC AGGATACAAA GGATCTGAGA 
CGCAAGAAGC TGGTCAAGCG TAGCAAGAGC AGCCTCATCA ACATGAAGGG CCTGGTGCTG 
CACACGCCCA ACGACGATGA CATATCCAAT TTGCTTAAAG AATTCACAGT CGACTTTTTA 
CTCAAAGGCT ACAACTATTT GGTAGAGGAG CTGCACTCCC AGCTGTTGTC CAATGCGAAG 
ATGCCCATTG ATACATCGCA TTTCTTCTGG CTTGTCACGT TCTTTCTCAA GTTTGCCGCC 
CAGCTGGAGC TGGACATGGA ACACATCGAT ACAATACTCA CCTTCGATGT GCTGAGCTTT 
CTAACCTACG AGGGTGTCAG CCTGTGCGAG CAGCTAGAGC TCAATGCACG ACAGGAGGGT 
GCTGATCTGA GACCCTATTT GCGTCGCATG CATCTGGTGG TCACGGCCAT ACGTGAGTTC 
CTGCAGGCCA TCGAAGCCTA CAACAAGGTC ACCCATCTCA GCGAGGATGA TCGCTATCGT 
CTACGCCAGC TCCAGCTACA GATAAGCGCT ACCACAGATC TGCGCTGCTT GTTTGTTCTG 
TTGCTGCGAC GATTTAATCC CAGCATCCAT TCCAAGCAGT ATTTACAAGA TCTGGTCGTG 
ACTAATCACA TTCTTTTGCT GATTTTGGAC AATGCTGCCA AATTGGAAGG CGGCCAGACC 
ATTGGACTCT CGGAGCACAT CTCACAATTT GCCACCTTGG AGGTGATGCA CTACTATGGC 
ATTCTCTTGG AGGACTTCAG CAACAATGGC GAATATGTCA ACGACTGCAT CTTTACCATG 
ATGCATCACA TTGGCGGCGA TCTGGACCAG GTTGGCGTTC TCTATCAGCC TATTATATTG 
AAGGCCTACT CTCGCATTTG GGAGGCCGAC TATGATATAT GCGATGACTG GAGCGATCTA 
ATTGAGTATG TTATTCACAA GTTTCTGAAC ACGCCACCCA AATCACCGAT GGCTATACCA 
ACCGCTTCGT TGACCGAGCT GACTAAGGAG CATAATCAGG AACACACTGC TTGTCCCTGG 
TCACAGGAGG ACATGGACTC GCTCTGTTGG TATTTTGTAC AGAGCAAGAG GCAAAACGAT 
GTGATTGGCA ATATTGCGAA GCTGTTTAGC AACAATGGCA ACAAGATAAA GACACGTATT 
TCTATCATTC AACAGCTGCT GCAACAGGAT ATCATTACGT TGCTGGAGTA CGATGATCTG 
ATGAAGTTCG AGGATGCCGA ATATCAGAGA ACTCTCCTGG CCACGCCGAC ATCGCTGACC 
ACCGATTCGG GCATTGAGTT GAAAGAAAGC GCCTATGGCA AACCCTCGGA TGATGTGCAG 
GTTCTGCTGG ATCTAATTAG AAAGGAGAAT AAGTCGCAGC ATTTGGTGTG GCTACAGAAA 
TTGTTGCTCG AGTGCTGCTA CGTCAAGATG ATGATCAAGT GCGGCAGCTG CCAGACGGAT 
GTGGAACCCA TCATGGAGCC GGTGGTCTAT CATTGCATGT TCAAGCAAAA GCCCATACCG 
GTGGTCCAGT GGAACAATGA ACAGTCGTCG ACGATGCTCT ATCAGCCCTT CATGCTGCTG 
CTGCACAAGC TTGGCATACA GCTGCCCGCG GATGCGGGCT TAATATTTGC GAGAATACCA 
GATTTTTGGA CTCCGGAAAC GATGTATGGG CTGGCAAAGA AGCTGGGACC GTTGGATAAA 
CTGACGCTCA AGTTTGATCC TCGCGATCTG GAGGATGCTC CCCCGTCGCG CCATCACACC 
GGCGCCCGCA ACTCGCTCAG CTCGATTAGC AGCCTGGAGG CTGATTTCGG CGATTCTGAG 
GGCCTGGCAC TCATACCCGA AGTGGATCCA GCTGTGGAGA AGGCAGCAGC AGCGGCTTCT 
GCTATACCAA ACAATGAAAT ATTTGCCTTG CCCAGGGTAA AGCACTGCAA CTCCATCATC 
AGATACACGC CGGATCCGAC ACCGCCCGTG CCCAGTTGGC TGCAGTTGGT CATGCGCAGC 
AAATGCAGCA AGCGACGCAG TCCAGCCACA GACGCCAGCG ATTGCACCAG TACCAGCACA 
ATGATTGCCG ATGACGAGAT CAAGTCATAT GCCTCCATGG CTCAGGCTTC ACATACAAAG 
CTGAACGCCG GCTATCCGAC TAGTACGTTG GTCTGTATGA ACAAGTTGAG CAACTGCAGC 
TTCGCGGCGC CACCGAATGA GAACAGCAGC AGCTCCGGCT GTGGCGGCAC TGCATCTTCG 
ATGTCAATGC CAAATATGCC GGATGGAAAT TCGGATGCGT TGATGAAGAC GTCGTTTGAG 
CGGCTGGCGG TGACGGGCGC ACGCTACTTG CGGCCCAGCA ATACGGATCA GGATTACAGC 
GCCTTGGTGG CATCGGTCTA CGAAAACGAG TTTGCCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015028465.1
CDS	981..4922
Translation	MDWLLATPQLQSVFSSLGSLVGGTYVVSPNALAILEEINHKLTYEDQTLRTFRRAIGFGQNVRVDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDLMYRTEVGRHTIFELNKLLYNSKEAFTDPKSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHFLMPRLQPGSGHQVSMQNTILWNLFIQSIDKLLLYLMTCPQRSLWGVTMVQLIALIYKDQHVSTLQKLLNLWFEASLSESSDDNESNTTPPKQASGDSSPMLTSDPTSDSSDNGSGGKKESCEERRQALREGTDATLHEVSRKGHEYQNAMASSNAANYILEGPCSAQQPWSDCEMQEYKQMTAVISEPLNLSQPADNVNYTTNANYARTTSADILTKTTSLKHEGFKPPAPRRNTLSAILSDNYAPLSFISAVKLGQKSPHAGQLQLIKGKCCPQKRECPSSQSEHSDCGYGTQMENPESISTSSNDDDGPQGKPQHQKPPCSSKHRSKQRIFAVPQDTKDLRRKKLVKRSKSSLINMKGLVLHTPNDDDISNLLKEFTVDFLLKGYNYLVEELHSQLLSNAKMPIDTSHFFWLVTFFLKFAAQLELDMEHIDTILTFDVLSFLTYEGVSLCEQLELNARQEGADLRPYLRRMHLVVTAIREFLQAIEAYNKVTHLSEDDRYRLRQLQLQISATTDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDNAAKLEGGQTIGLSEHISQFATLEVMHYYGILLEDFSNNGEYVNDCIFTMMHHIGGDLDQVGVLYQPIILKAYSRIWEADYDICDDWSDLIEYVIHKFLNTPPKSPMAIPTASLTELTKEHNQEHTACPWSQEDMDSLCWYFVQSKRQNDVIGNIAKLFSNNGNKIKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLATPTSLTTDSGIELKESAYGKPSDDVQVLLDLIRKENKSQHLVWLQKLLLECCYVKMMIKCGSCQTDVEPIMEPVVYHCMFKQKPIPVVQWNNEQSSTMLYQPFMLLLHKLGIQLPADAGLIFARIPDFWTPETMYGLAKKLGPLDKLTLKFDPRDLEDAPPSRHHTGARNSLSSISSLEADFGDSEGLALIPEVDPAVEKAAAAASAIPNNEIFALPRVKHCNSIIRYTPDPTPPVPSWLQLVMRSKCSKRRSPATDASDCTSTSTMIADDEIKSYASMAQASHTKLNAGYPTSTLVCMNKLSNCSFAAPPNENSSSSGCGGTASSMSMPNMPDGNSDALMKTSFERLAVTGARYLRPSNTDQDYSALVASVYENEFAK

Target ORF information:

RefSeq Version	XM_015172979.1
Organism	Drosophila virilis
Definition	Drosophila virilis tim, transcript variant B (Dvir\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015172979.1

ORF Insert Sequence:

ATGGACTGGT TACTCGCAAC ACCTCAGCTT CAGAGCGTCT TCTCCTCGTT GGGCTCCCTT 
GTTGGCGGCA CCTACGTGGT CAGTCCCAAT GCATTGGCAA TTTTGGAGGA GATTAACCAT 
AAACTTACCT ATGAGGATCA AACGCTGCGC ACTTTCCGTC GCGCCATCGG CTTTGGCCAG 
AATGTGCGAG TCGATCTAAT ACCATTGCTC GAGAATGCCA AGGATGATGC GGTGCTCGAG 
TCAGTCATAC GGATATTGGT TAATCTTACC GTGCCGGTGG AGTGCCTGTT CTCGGTGGAT 
CTCATGTACC GCACCGAGGT AGGGCGTCAC ACAATTTTCG AGCTGAACAA GCTGCTCTAC 
AACAGCAAGG AGGCCTTCAC CGATCCGAAG AGCACCAAGA GCGTCGTCGA GTATATGAAA 
CACATTCTGG AATCGGATCC AAAGCTATCG CCGCACAAAT GCGATCAGAT CAACAATTGT 
CTCCTGTTGT TGCGCAACAT TTTGCACATA CCGGAGACGC ATGCACATTT CCTGATGCCC 
AGGCTGCAGC CGGGCAGCGG CCATCAGGTG TCCATGCAGA ACACCATATT ATGGAACCTA 
TTCATACAGA GCATTGATAA GTTGCTCCTA TATCTGATGA CCTGTCCGCA GCGTTCGCTC 
TGGGGCGTGA CCATGGTGCA GCTGATTGCA CTGATCTACA AGGATCAGCA TGTGAGCACC 
TTGCAGAAGC TGCTGAACTT GTGGTTTGAG GCCTCACTGT CGGAGAGTTC GGATGACAAT 
GAGAGTAACA CAACGCCACC CAAACAGGCC AGCGGCGACT CCAGTCCCAT GCTCACCTCA 
GATCCCACAT CGGACTCTTC GGACAATGGC AGCGGCGGCA AGAAGGAAAG CTGCGAGGAG 
CGACGTCAGG CTCTGCGCGA GGGCACCGAT GCCACGCTAC ATGAGGTCAG TCGCAAAGGG 
CATGAATATC AGAATGCCAT GGCCAGCTCG AATGCGGCCA ACTATATACT CGAGGGACCC 
TGCTCGGCCC AACAGCCGTG GTCCGACTGT GAAATGCAGG AGTACAAACA GATGACTGCC 
GTCATCAGCG AACCATTAAA TCTTTCACAA CCAGCTGACA ATGTCAACTA CACTACCAAT 
GCGAATTACG CTCGCACCAC AAGCGCAGAT ATCTTGACCA AAACGACTAG CCTAAAACAT 
GAGGGCTTTA AGCCGCCCGC ACCACGTCGA AACACGCTCT CGGCCATATT GTCGGACAAT 
TACGCACCAC TCTCTTTTAT TTCGGCCGTC AAGCTGGGCC AGAAGTCACC GCACGCGGGT 
CAACTGCAGC TAATCAAGGG CAAATGCTGT CCGCAGAAGC GTGAGTGCCC ATCCTCACAG 
TCGGAGCACT CCGACTGCGG CTATGGCACC CAGATGGAGA ATCCAGAGTC CATATCCACA 
TCCAGCAATG ATGATGATGG TCCCCAGGGC AAGCCGCAAC ATCAGAAGCC GCCGTGCAGT 
AGTAAGCATC GCAGCAAACA ACGCATCTTT GCAGTGCCGC AGGATACAAA GGATCTGAGA 
CGCAAGAAGC TGGTCAAGCG TAGCAAGAGC AGCCTCATCA ACATGAAGGG CCTGGTGCTG 
CACACGCCCA ACGACGATGA CATATCCAAT TTGCTTAAAG AATTCACAGT CGACTTTTTA 
CTCAAAGGCT ACAACTATTT GGTAGAGGAG CTGCACTCCC AGCTGTTGTC CAATGCGAAG 
ATGCCCATTG ATACATCGCA TTTCTTCTGG CTTGTCACGT TCTTTCTCAA GTTTGCCGCC 
CAGCTGGAGC TGGACATGGA ACACATCGAT ACAATACTCA CCTTCGATGT GCTGAGCTTT 
CTAACCTACG AGGGTGTCAG CCTGTGCGAG CAGCTAGAGC TCAATGCACG ACAGGAGGGT 
GCTGATCTGA GACCCTATTT GCGTCGCATG CATCTGGTGG TCACGGCCAT ACGTGAGTTC 
CTGCAGGCCA TCGAAGCCTA CAACAAGGTC ACCCATCTCA GCGAGGATGA TCGCTATCGT 
CTACGCCAGC TCCAGCTACA GATAAGCGCT ACCACAGATC TGCGCTGCTT GTTTGTTCTG 
TTGCTGCGAC GATTTAATCC CAGCATCCAT TCCAAGCAGT ATTTACAAGA TCTGGTCGTG 
ACTAATCACA TTCTTTTGCT GATTTTGGAC AATGCTGCCA AATTGGAAGG CGGCCAGACC 
ATTGGACTCT CGGAGCACAT CTCACAATTT GCCACCTTGG AGGTGATGCA CTACTATGGC 
ATTCTCTTGG AGGACTTCAG CAACAATGGC GAATATGTCA ACGACTGCAT CTTTACCATG 
ATGCATCACA TTGGCGGCGA TCTGGACCAG GTTGGCGTTC TCTATCAGCC TATTATATTG 
AAGGCCTACT CTCGCATTTG GGAGGCCGAC TATGATATAT GCGATGACTG GAGCGATCTA 
ATTGAGTATG TTATTCACAA GTTTCTGAAC ACGCCACCCA AATCACCGAT GGCTATACCA 
ACCGCTTCGT TGACCGAGCT GACTAAGGAG CATAATCAGG AACACACTGC TTGTCCCTGG 
TCACAGGAGG ACATGGACTC GCTCTGTTGG TATTTTGTAC AGAGCAAGAG GCAAAACGAT 
GTGATTGGCA ATATTGCGAA GCTGTTTAGC AACAATGGCA ACAAGATAAA GACACGTATT 
TCTATCATTC AACAGCTGCT GCAACAGGAT ATCATTACGT TGCTGGAGTA CGATGATCTG 
ATGAAGTTCG AGGATGCCGA ATATCAGAGA ACTCTCCTGG CCACGCCGAC ATCGCTGACC 
ACCGATTCGG GCATTGAGTT GAAAGAAAGC GCCTATGGCA AACCCTCGGA TGATGTGCAG 
GTTCTGCTGG ATCTAATTAG AAAGGAGAAT AAGTCGCAGC ATTTGGTGTG GCTACAGAAA 
TTGTTGCTCG AGTGCTGCTA CGTCAAGATG ATGATCAAGT GCGGCAGCTG CCAGACGGAT 
GTGGAACCCA TCATGGAGCC GGTGGTCTAT CATTGCATGT TCAAGCAAAA GCCCATACCG 
GTGGTCCAGT GGAACAATGA ACAGTCGTCG ACGATGCTCT ATCAGCCCTT CATGCTGCTG 
CTGCACAAGC TTGGCATACA GCTGCCCGCG GATGCGGGCT TAATATTTGC GAGAATACCA 
GATTTTTGGA CTCCGGAAAC GATGTATGGG CTGGCAAAGA AGCTGGGACC GTTGGATAAA 
CTGACGCTCA AGTTTGATCC TCGCGATCTG GAGGATGCTC CCCCGTCGCG CCATCACACC 
GGCGCCCGCA ACTCGCTCAG CTCGATTAGC AGCCTGGAGG CTGATTTCGG CGATTCTGAG 
GGCCTGGCAC TCATACCCGA AGTGGATCCA GCTGTGGAGA AGGCAGCAGC AGCGGCTTCT 
GCTATACCAA ACAATGAAAT ATTTGCCTTG CCCAGGGTAA AGCACTGCAA CTCCATCATC 
AGATACACGC CGGATCCGAC ACCGCCCGTG CCCAGTTGGC TGCAGTTGGT CATGCGCAGC 
AAATGCAGCA AGCGACGCAG TCCAGCCACA GACGCCAGCG ATTGCACCAG TACCAGCACA 
ATGATTGCCG ATGACGAGAT CAAGTCATAT GCCTCCATGG CTCAGGCTTC ACATACAAAG 
CTGAACGCCG GCTATCCGAC TAGTACGTTG GTCTGTATGA ACAAGTTGAG CAACTGCAGC 
TTCGCGGCGC CACCGAATGA GAACAGCAGC AGCTCCGGCT GTGGCGGCAC TGCATCTTCG 
ATGTCAATGC CAAATATGCC GGATGGAAAT TCGGATGCGT TGATGAAGAC GTCGTTTGAG 
CGGCTGGCGG TGACGGGCGC ACGCTACTTG CGGCCCAGCA ATACGGATCA GGATTACAGC 
GCCTTGGTGG CATCGGTCTA CGAAAACGAG TTTGCCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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Dvir\tim cDNA ORF clone, Drosophila virilis

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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