Dsim\tim cDNA ORF clone, Drosophila simulans

Gene
Clones
gRNAs

Summary

Gene Symbol	Dsim\tim
Entrez Gene ID	6730875
Full Name	GD22753 gene product from transcript GD22753-RD
Synonyms	Dsim\GD22753,Dsim_GD22753,GD22753,TIM,dsim_GLEANR_6496,tim
Gene Type	protein-coding
Organism	Drosophila simulans

mRNA and Protein(s)

mRNA	Protein	Name
XM_016179061.1	XP_016023295.1	tim, isoform B
XM_016179065.1	XP_016023299.1	tim, isoform F
XM_016179063.1	XP_016023297.1	tim, isoform D
XM_016179062.1	XP_016023296.1	tim, isoform C
XM_016179064.1	XP_016023298.1	tim, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Hu TT, Eisen MB, Thornton KR, Andolfatto P
Genome research23(1)89-98(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dsim\tim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dsim\tim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODg18785	XM_016179061.1 Latest version!	Drosophila simulans tim, transcript variant B (Dsim\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODg18786	XM_016179062.1 Latest version!	Drosophila simulans tim, transcript variant C (Dsim\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODg18787	XM_016179064.1 Latest version!	Drosophila simulans tim, transcript variant E (Dsim\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODg18787	XM_016179065.1 Latest version!	Drosophila simulans tim, transcript variant F (Dsim\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODg18787	XM_016179063.1 Latest version!	Drosophila simulans tim, transcript variant D (Dsim\tim), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dsim\tim ( XM_016179061.1 ) cDNA ORF clone, Drosophila simulans -> XP_016023295.1 Drosophila simulans tim, transcript variant B (Dsim\tim), mRNA.

Price & Availability↑

CloneID	ODg18785
Clone ID Related Accession (Same CDS sequence)	XM_016179061.1
Accession Version	XM_016179061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	tim, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGGTGC CCATTGACAC ATCGCACTTC 
TTCTGGCTGG TCACCTACTT CCTGAAGTTT GCCGCCCAAC TGGAGCTGGA CATGGAGCAC 
ATCGACACCA TTCTCACCTA CGATGTTTTG AGCTACTTGA CCTATGAGGG TGTTTCCCTT 
TGTGAACAAC TGGAACTGAA TGCCCGACAG GAGGGCAGTG ACCTGAAGCC CTATCTGAGG 
CGGATGCACT TGGTGGTGAC GGCCATCCGG GAGTTCCTCC AGGCCATCGA CACATACAAC 
AAAGTGACCC ATCTGAACGA GGACGACAAG GCCCATTTGA GGCAGCTCCA GCTGCAGATT 
AGCGAGATGT CCGATCTGAG GTGCCTTTTT GTCCTGCTGC TTAGGCGCTT CAATCCCAGC 
ATCCATTCCA AGCAGTATCT CCAGGATCTG GTGGTTACCA ATCACATCCT CCTCCTCATT 
CTGGACAGTT CGGCCAAACT GGGTGGATGT CAAACCATTC GCCTGTCGGA GCACATTACA 
CAGTTTGCCA CGCTGGAGGT GATGCACTAC TATGGCATCC TCTTGGAGGA CTTCAACAAC 
AACGGAGAGT TTGTCAATGA CTGCATCTTC ACCATGATGC ATCACATAGG TGGCGATCTG 
GGCCAGATTG GGGTTCTATT CCAACCAATT ATTTTGAAAA CCTATTCAAG AATTTGGGAA 
GCGGACTATG AACTGTGCGA TGACTGGTCT GATCTTATCG AGTATGTGAT TCACAAGTTC 
ATGAATACTC CTCCGAAGTC GCCACTCACC ATTCCCACAA CTTCCTTAAC GGAAATGACC 
AAGGAACACA ACCAGGAGCA TACCGTTTGC TCATGGTCGC AGGAGGAAAT GGATACACTG 
TATTGGTATT ATGTGCAGAG CAAGAAGAAC AACGATATTG TGGGAAAGAT AGTTAAGCTC 
TTCAGCAACA ACGGCAACAA GCTGAAAACC CGGATTTCTA TTATCCAACA ACTTTTGCAA 
CAAGACATTA TTACCCTGTT GGAGTACGAT GATCTGATGA AGTTCGAGGA TGCGGAGTAT 
CAGAGAACTT TGCTGACCAC TCCCACTTCC GCAACCACAG AGTCTGGAAT TGAGATTAAG 
GAGTGCGCCT ACGGCAAACC CTCAGATGAT GTGCAGATCC TGCTGGACCT GATCATCAAG 
GAAAACAAAG CGCAGCATTT GTTATGGCTG CAGAGGATCC TCATCGAGTG CTGCTTCGTC 
AAGCTGACCC TGCGGAGTGG TCTCAAGGTT CCGGAAGGCG ATCACATCAT GGAGCCGGTG 
GCCTATCACT GCATCTGCAA GCAGAAGTCC ATTCCGGTGG TGCAGTGGAA CAACGAGCAA 
TCCACTACGA TGCTGTACCA GCCCTTTGTG CTGCTGCTCC ACAAACTGGG CATTCAGCTG 
CCGGCGGACG CGGGCTCGAT CTTCGCCAGA ATTCCGGACT ACTGGACACC GGAGACCATG 
TACGGACTGG CCAAGAAGCT GGGACCGCTG GACAAACTCA ACCTAAAGTT CGACACCAGC 
GAACTGGAGG ATGCGACGGC GTCGAGTCCG TCGCGGTACC ACCACACCGG ACCCCGCAAC 
TCGCTCAGCT CGGTGAGCAG CCTGGACGTG GACCTCGGCG AGCCCGAGGA GCTGGCCCTC 
ATACCCGAGG TGGATGCGGC CGTGGAGAAG GCACACGCCA TGGCGTCCAC GCCATCGCCC 
AGCGAGATGT TCGCGGTGCC CAAGACGAAG CACTGCAACT CGATCATCAG ATACACACCC 
GATCCCACGC CTCCAGTGCC CAACTGGCTG CAGTTGGTCA TGCGCAGCAA ATGCAATCAG 
CGCACAGGTC CGGCTGGTGA TCCCAGCGAT TGCGTGGGCT CCTCGTCGAC AACAGTGGAC 
GATGAGGGAT TGGGCAAGTC CATCAGTGCA GCCACTTCGC AGGCGGCGAG CACCTCCATG 
AGCACGGTTA ATCCCACAAC CACTTTGAGC CTCAATATGC TGAACACCTT CATGGGAAGC 
CACAACGAGA ACAGCAGCAG TTCCGGATGC GGAGGCACCG TGTCCTCCCT GTCCATGGTG 
GCTCTGATGA GCACTGGCGC GGTCGGCGGA GGAGGCAACA CCTCTGGACT GGACATGGAT 
GTGGACGCCT CCATGAAGTC GTCGTTCGAG CGACTGGAGG TCAACGGATC GCACTTCTCG 
CGGGCCAACA ACTTGGACCA GGAGTACAGT GCTATGGTGG CATCTGTGTA CGAAAAGGAG 
AAGGAATTAA ACAGCGACAA TGTCTCTTTG GCCTCGGACC TGACCAGAAT GTATGTGAGC 
GATGAGGACG ATCGACTTGA GCGAACCGAG ATCCGGGTGC CCCACTATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016023295.1
CDS	303..4496
Translation	MDWLLATPQLYSAFSSLGCLEGDTYVVNPNALAILEEINYKLTYEDQTLRTFRRAIGFGQNVRSDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDVMYRTDVGRHTIFELNKLLYTSKEAFTEARSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHCVMPMMQSMPHGISMQNTILWNLFIQSIDKLLLYLMTCPQRAFWGVTMVQLIALIYKDQHVSTLQKLLSLWFEASLSESSEDNESNTSPPKQGSGDSSPMLTSDPTSDSSDNGSNGRGMGGGMREGTAATLQEVSRKGQEYQNAMARVPADKPDGSEEASDMTGNDSEQPGSPEQSQPAGESMDDGDYEDQRHSQLNEHGEEDEDEDEVEEEEYLQLGPASEPLNLTQQPADKVNNTTSPTSSPPQGCLGTEPFKPPPPLPVRASTSAHAQMQKFNESSYASHVSAVKLGQKSPHAGQLQLTKGKCCPQKRECPSSQSELSDCGYGTQVENQESISTSSNDDDGPQGKPQHQKPPCNTKPRNKPRTIMSPMDKKELRRKKLVKRSKSSLINMKGLVQHTPTDDDISNLLKEFTVDFLLKGYSYLVEELHMQLLSNAVPIDTSHFFWLVTYFLKFAAQLELDMEHIDTILTYDVLSYLTYEGVSLCEQLELNARQEGSDLKPYLRRMHLVVTAIREFLQAIDTYNKVTHLNEDDKAHLRQLQLQISEMSDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDSSAKLGGCQTIRLSEHITQFATLEVMHYYGILLEDFNNNGEFVNDCIFTMMHHIGGDLGQIGVLFQPIILKTYSRIWEADYELCDDWSDLIEYVIHKFMNTPPKSPLTIPTTSLTEMTKEHNQEHTVCSWSQEEMDTLYWYYVQSKKNNDIVGKIVKLFSNNGNKLKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLTTPTSATTESGIEIKECAYGKPSDDVQILLDLIIKENKAQHLLWLQRILIECCFVKLTLRSGLKVPEGDHIMEPVAYHCICKQKSIPVVQWNNEQSTTMLYQPFVLLLHKLGIQLPADAGSIFARIPDYWTPETMYGLAKKLGPLDKLNLKFDTSELEDATASSPSRYHHTGPRNSLSSVSSLDVDLGEPEELALIPEVDAAVEKAHAMASTPSPSEMFAVPKTKHCNSIIRYTPDPTPPVPNWLQLVMRSKCNQRTGPAGDPSDCVGSSSTTVDDEGLGKSISAATSQAASTSMSTVNPTTTLSLNMLNTFMGSHNENSSSSGCGGTVSSLSMVALMSTGAVGGGGNTSGLDMDVDASMKSSFERLEVNGSHFSRANNLDQEYSAMVASVYEKEKELNSDNVSLASDLTRMYVSDEDDRLERTEIRVPHYH

Target ORF information:

RefSeq Version	XM_016179061.1
Organism	Drosophila simulans
Definition	Drosophila simulans tim, transcript variant B (Dsim\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016179061.1

ORF Insert Sequence:

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGGTGC CCATTGACAC ATCGCACTTC 
TTCTGGCTGG TCACCTACTT CCTGAAGTTT GCCGCCCAAC TGGAGCTGGA CATGGAGCAC 
ATCGACACCA TTCTCACCTA CGATGTTTTG AGCTACTTGA CCTATGAGGG TGTTTCCCTT 
TGTGAACAAC TGGAACTGAA TGCCCGACAG GAGGGCAGTG ACCTGAAGCC CTATCTGAGG 
CGGATGCACT TGGTGGTGAC GGCCATCCGG GAGTTCCTCC AGGCCATCGA CACATACAAC 
AAAGTGACCC ATCTGAACGA GGACGACAAG GCCCATTTGA GGCAGCTCCA GCTGCAGATT 
AGCGAGATGT CCGATCTGAG GTGCCTTTTT GTCCTGCTGC TTAGGCGCTT CAATCCCAGC 
ATCCATTCCA AGCAGTATCT CCAGGATCTG GTGGTTACCA ATCACATCCT CCTCCTCATT 
CTGGACAGTT CGGCCAAACT GGGTGGATGT CAAACCATTC GCCTGTCGGA GCACATTACA 
CAGTTTGCCA CGCTGGAGGT GATGCACTAC TATGGCATCC TCTTGGAGGA CTTCAACAAC 
AACGGAGAGT TTGTCAATGA CTGCATCTTC ACCATGATGC ATCACATAGG TGGCGATCTG 
GGCCAGATTG GGGTTCTATT CCAACCAATT ATTTTGAAAA CCTATTCAAG AATTTGGGAA 
GCGGACTATG AACTGTGCGA TGACTGGTCT GATCTTATCG AGTATGTGAT TCACAAGTTC 
ATGAATACTC CTCCGAAGTC GCCACTCACC ATTCCCACAA CTTCCTTAAC GGAAATGACC 
AAGGAACACA ACCAGGAGCA TACCGTTTGC TCATGGTCGC AGGAGGAAAT GGATACACTG 
TATTGGTATT ATGTGCAGAG CAAGAAGAAC AACGATATTG TGGGAAAGAT AGTTAAGCTC 
TTCAGCAACA ACGGCAACAA GCTGAAAACC CGGATTTCTA TTATCCAACA ACTTTTGCAA 
CAAGACATTA TTACCCTGTT GGAGTACGAT GATCTGATGA AGTTCGAGGA TGCGGAGTAT 
CAGAGAACTT TGCTGACCAC TCCCACTTCC GCAACCACAG AGTCTGGAAT TGAGATTAAG 
GAGTGCGCCT ACGGCAAACC CTCAGATGAT GTGCAGATCC TGCTGGACCT GATCATCAAG 
GAAAACAAAG CGCAGCATTT GTTATGGCTG CAGAGGATCC TCATCGAGTG CTGCTTCGTC 
AAGCTGACCC TGCGGAGTGG TCTCAAGGTT CCGGAAGGCG ATCACATCAT GGAGCCGGTG 
GCCTATCACT GCATCTGCAA GCAGAAGTCC ATTCCGGTGG TGCAGTGGAA CAACGAGCAA 
TCCACTACGA TGCTGTACCA GCCCTTTGTG CTGCTGCTCC ACAAACTGGG CATTCAGCTG 
CCGGCGGACG CGGGCTCGAT CTTCGCCAGA ATTCCGGACT ACTGGACACC GGAGACCATG 
TACGGACTGG CCAAGAAGCT GGGACCGCTG GACAAACTCA ACCTAAAGTT CGACACCAGC 
GAACTGGAGG ATGCGACGGC GTCGAGTCCG TCGCGGTACC ACCACACCGG ACCCCGCAAC 
TCGCTCAGCT CGGTGAGCAG CCTGGACGTG GACCTCGGCG AGCCCGAGGA GCTGGCCCTC 
ATACCCGAGG TGGATGCGGC CGTGGAGAAG GCACACGCCA TGGCGTCCAC GCCATCGCCC 
AGCGAGATGT TCGCGGTGCC CAAGACGAAG CACTGCAACT CGATCATCAG ATACACACCC 
GATCCCACGC CTCCAGTGCC CAACTGGCTG CAGTTGGTCA TGCGCAGCAA ATGCAATCAG 
CGCACAGGTC CGGCTGGTGA TCCCAGCGAT TGCGTGGGCT CCTCGTCGAC AACAGTGGAC 
GATGAGGGAT TGGGCAAGTC CATCAGTGCA GCCACTTCGC AGGCGGCGAG CACCTCCATG 
AGCACGGTTA ATCCCACAAC CACTTTGAGC CTCAATATGC TGAACACCTT CATGGGAAGC 
CACAACGAGA ACAGCAGCAG TTCCGGATGC GGAGGCACCG TGTCCTCCCT GTCCATGGTG 
GCTCTGATGA GCACTGGCGC GGTCGGCGGA GGAGGCAACA CCTCTGGACT GGACATGGAT 
GTGGACGCCT CCATGAAGTC GTCGTTCGAG CGACTGGAGG TCAACGGATC GCACTTCTCG 
CGGGCCAACA ACTTGGACCA GGAGTACAGT GCTATGGTGG CATCTGTGTA CGAAAAGGAG 
AAGGAATTAA ACAGCGACAA TGTCTCTTTG GCCTCGGACC TGACCAGAAT GTATGTGAGC 
GATGAGGACG ATCGACTTGA GCGAACCGAG ATCCGGGTGC CCCACTATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\tim ( XM_016179062.1 ) cDNA ORF clone, Drosophila simulans -> XP_016023296.1 Drosophila simulans tim, transcript variant C (Dsim\tim), mRNA.

Price & Availability↑

CloneID	ODg18786
Clone ID Related Accession (Same CDS sequence)	XM_016179062.1
Accession Version	XM_016179062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	tim, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGACATGGAG 
CACATCGACA CCATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTTTCC 
CTTTGTGAAC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTG 
AGGCGGATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT CGACACATAC 
AACAAAGTGA CCCATCTGAA CGAGGACGAC AAGGCCCATT TGAGGCAGCT CCAGCTGCAG 
ATTAGCGAGA TGTCCGATCT GAGGTGCCTT TTTGTCCTGC TGCTTAGGCG CTTCAATCCC 
AGCATCCATT CCAAGCAGTA TCTCCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTCCTC 
ATTCTGGACA GTTCGGCCAA ACTGGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATT 
ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TCCTCTTGGA GGACTTCAAC 
AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT AGGTGGCGAT 
CTGGGCCAGA TTGGGGTTCT ATTCCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCCA CAACTTCCTT AACGGAAATG 
ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCATGGT CGCAGGAGGA AATGGATACA 
CTGTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCCGGATTT CTATTATCCA ACAACTTTTG 
CAACAAGACA TTATTACCCT GTTGGAGTAC GATGATCTGA TGAAGTTCGA GGATGCGGAG 
TATCAGAGAA CTTTGCTGAC CACTCCCACT TCCGCAACCA CAGAGTCTGG AATTGAGATT 
AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTGCAGA TCCTGCTGGA CCTGATCATC 
AAGGAAAACA AAGCGCAGCA TTTGTTATGG CTGCAGAGGA TCCTCATCGA GTGCTGCTTC 
GTCAAGCTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
GTGGCCTATC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
CAATCCACTA CGATGCTGTA CCAGCCCTTT GTGCTGCTGC TCCACAAACT GGGCATTCAG 
CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACC 
ATGTACGGAC TGGCCAAGAA GCTGGGACCG CTGGACAAAC TCAACCTAAA GTTCGACACC 
AGCGAACTGG AGGATGCGAC GGCGTCGAGT CCGTCGCGGT ACCACCACAC CGGACCCCGC 
AACTCGCTCA GCTCGGTGAG CAGCCTGGAC GTGGACCTCG GCGAGCCCGA GGAGCTGGCC 
CTCATACCCG AGGTGGATGC GGCCGTGGAG AAGGCACACG CCATGGCGTC CACGCCATCG 
CCCAGCGAGA TGTTCGCGGT GCCCAAGACG AAGCACTGCA ACTCGATCAT CAGATACACA 
CCCGATCCCA CGCCTCCAGT GCCCAACTGG CTGCAGTTGG TCATGCGCAG CAAATGCAAT 
CAGCGCACAG GTCCGGCTGG TGATCCCAGC GATTGCGTGG GCTCCTCGTC GACAACAGTG 
GACGATGAGG GATTGGGCAA GTCCATCAGT GCAGCCACTT CGCAGGCGGC GAGCACCTCC 
ATGAGCACGG TTAATCCCAC AACCACTTTG AGCCTCAATA TGCTGAACAC CTTCATGGGA 
AGCCACAACG AGAACAGCAG CAGTTCCGGA TGCGGAGGCA CCGTGTCCTC CCTGTCCATG 
GTGGCTCTGA TGAGCACTGG CGCGGTCGGC GGAGGAGGCA ACACCTCTGG ACTGGACATG 
GATGTGGACG CCTCCATGAA GTCGTCGTTC GAGCGACTGG AGGTCAACGG ATCGCACTTC 
TCGCGGGCCA ACAACTTGGA CCAGGAGTAC AGTGCTATGG TGGCATCTGT GTACGAAAAG 
GAGAAGGAAT TAAACAGCGA CAATGTCTCT TTGGCCTCGG ACCTGACCAG AATGTATGTG 
AGCGATGAGG ACGATCGACT TGAGCGAACC GAGATCCGGG TGCCCCACTA TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016023296.1
CDS	303..4499
Translation	MDWLLATPQLYSAFSSLGCLEGDTYVVNPNALAILEEINYKLTYEDQTLRTFRRAIGFGQNVRSDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDVMYRTDVGRHTIFELNKLLYTSKEAFTEARSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHCVMPMMQSMPHGISMQNTILWNLFIQSIDKLLLYLMTCPQRAFWGVTMVQLIALIYKDQHVSTLQKLLSLWFEASLSESSEDNESNTSPPKQGSGDSSPMLTSDPTSDSSDNGSNGRGMGGGMREGTAATLQEVSRKGQEYQNAMARVPADKPDGSEEASDMTGNDSEQPGSPEQSQPAGESMDDGDYEDQRHSQLNEHGEEDEDEDEVEEEEYLQLGPASEPLNLTQQPADKVNNTTSPTSSPPQGCLGTEPFKPPPPLPVRASTSAHAQMQKFNESSYASHVSAVKLGQKSPHAGQLQLTKGKCCPQKRECPSSQSELSDCGYGTQVENQESISTSSNDDDGPQGKPQHQKPPCNTKPRNKPRTIMSPMDKKELRRKKLVKRSKSSLINMKGLVQHTPTDDDISNLLKEFTVDFLLKGYSYLVEELHMQLLSNAKVPIDTSHFFWLVTYFLKFAAQLELDMEHIDTILTYDVLSYLTYEGVSLCEQLELNARQEGSDLKPYLRRMHLVVTAIREFLQAIDTYNKVTHLNEDDKAHLRQLQLQISEMSDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDSSAKLGGCQTIRLSEHITQFATLEVMHYYGILLEDFNNNGEFVNDCIFTMMHHIGGDLGQIGVLFQPIILKTYSRIWEADYELCDDWSDLIEYVIHKFMNTPPKSPLTIPTTSLTEMTKEHNQEHTVCSWSQEEMDTLYWYYVQSKKNNDIVGKIVKLFSNNGNKLKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLTTPTSATTESGIEIKECAYGKPSDDVQILLDLIIKENKAQHLLWLQRILIECCFVKLTLRSGLKVPEGDHIMEPVAYHCICKQKSIPVVQWNNEQSTTMLYQPFVLLLHKLGIQLPADAGSIFARIPDYWTPETMYGLAKKLGPLDKLNLKFDTSELEDATASSPSRYHHTGPRNSLSSVSSLDVDLGEPEELALIPEVDAAVEKAHAMASTPSPSEMFAVPKTKHCNSIIRYTPDPTPPVPNWLQLVMRSKCNQRTGPAGDPSDCVGSSSTTVDDEGLGKSISAATSQAASTSMSTVNPTTTLSLNMLNTFMGSHNENSSSSGCGGTVSSLSMVALMSTGAVGGGGNTSGLDMDVDASMKSSFERLEVNGSHFSRANNLDQEYSAMVASVYEKEKELNSDNVSLASDLTRMYVSDEDDRLERTEIRVPHYH

Target ORF information:

RefSeq Version	XM_016179062.1
Organism	Drosophila simulans
Definition	Drosophila simulans tim, transcript variant C (Dsim\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016179062.1

ORF Insert Sequence:

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGACATGGAG 
CACATCGACA CCATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTTTCC 
CTTTGTGAAC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTG 
AGGCGGATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT CGACACATAC 
AACAAAGTGA CCCATCTGAA CGAGGACGAC AAGGCCCATT TGAGGCAGCT CCAGCTGCAG 
ATTAGCGAGA TGTCCGATCT GAGGTGCCTT TTTGTCCTGC TGCTTAGGCG CTTCAATCCC 
AGCATCCATT CCAAGCAGTA TCTCCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTCCTC 
ATTCTGGACA GTTCGGCCAA ACTGGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATT 
ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TCCTCTTGGA GGACTTCAAC 
AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT AGGTGGCGAT 
CTGGGCCAGA TTGGGGTTCT ATTCCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCCA CAACTTCCTT AACGGAAATG 
ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCATGGT CGCAGGAGGA AATGGATACA 
CTGTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCCGGATTT CTATTATCCA ACAACTTTTG 
CAACAAGACA TTATTACCCT GTTGGAGTAC GATGATCTGA TGAAGTTCGA GGATGCGGAG 
TATCAGAGAA CTTTGCTGAC CACTCCCACT TCCGCAACCA CAGAGTCTGG AATTGAGATT 
AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTGCAGA TCCTGCTGGA CCTGATCATC 
AAGGAAAACA AAGCGCAGCA TTTGTTATGG CTGCAGAGGA TCCTCATCGA GTGCTGCTTC 
GTCAAGCTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
GTGGCCTATC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
CAATCCACTA CGATGCTGTA CCAGCCCTTT GTGCTGCTGC TCCACAAACT GGGCATTCAG 
CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACC 
ATGTACGGAC TGGCCAAGAA GCTGGGACCG CTGGACAAAC TCAACCTAAA GTTCGACACC 
AGCGAACTGG AGGATGCGAC GGCGTCGAGT CCGTCGCGGT ACCACCACAC CGGACCCCGC 
AACTCGCTCA GCTCGGTGAG CAGCCTGGAC GTGGACCTCG GCGAGCCCGA GGAGCTGGCC 
CTCATACCCG AGGTGGATGC GGCCGTGGAG AAGGCACACG CCATGGCGTC CACGCCATCG 
CCCAGCGAGA TGTTCGCGGT GCCCAAGACG AAGCACTGCA ACTCGATCAT CAGATACACA 
CCCGATCCCA CGCCTCCAGT GCCCAACTGG CTGCAGTTGG TCATGCGCAG CAAATGCAAT 
CAGCGCACAG GTCCGGCTGG TGATCCCAGC GATTGCGTGG GCTCCTCGTC GACAACAGTG 
GACGATGAGG GATTGGGCAA GTCCATCAGT GCAGCCACTT CGCAGGCGGC GAGCACCTCC 
ATGAGCACGG TTAATCCCAC AACCACTTTG AGCCTCAATA TGCTGAACAC CTTCATGGGA 
AGCCACAACG AGAACAGCAG CAGTTCCGGA TGCGGAGGCA CCGTGTCCTC CCTGTCCATG 
GTGGCTCTGA TGAGCACTGG CGCGGTCGGC GGAGGAGGCA ACACCTCTGG ACTGGACATG 
GATGTGGACG CCTCCATGAA GTCGTCGTTC GAGCGACTGG AGGTCAACGG ATCGCACTTC 
TCGCGGGCCA ACAACTTGGA CCAGGAGTAC AGTGCTATGG TGGCATCTGT GTACGAAAAG 
GAGAAGGAAT TAAACAGCGA CAATGTCTCT TTGGCCTCGG ACCTGACCAG AATGTATGTG 
AGCGATGAGG ACGATCGACT TGAGCGAACC GAGATCCGGG TGCCCCACTA TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\tim ( XM_016179065.1 ) cDNA ORF clone, Drosophila simulans -> XP_016023299.1 Drosophila simulans tim, transcript variant F (Dsim\tim), mRNA.

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CloneID	ODg18787
Clone ID Related Accession (Same CDS sequence)	XM_016179065.1 , XM_016179063.1 , XM_016179064.1
Accession Version	XM_016179065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	tim, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAACA AGAAGGTGCC CATTGACACA 
TCGCACTTCT TCTGGCTGGT CACCTACTTC CTGAAGTTTG CCGCCCAACT GGAGCTGGAC 
ATGGAGCACA TCGACACCAT TCTCACCTAC GATGTTTTGA GCTACTTGAC CTATGAGGGT 
GTTTCCCTTT GTGAACAACT GGAACTGAAT GCCCGACAGG AGGGCAGTGA CCTGAAGCCC 
TATCTGAGGC GGATGCACTT GGTGGTGACG GCCATCCGGG AGTTCCTCCA GGCCATCGAC 
ACATACAACA AAGTGACCCA TCTGAACGAG GACGACAAGG CCCATTTGAG GCAGCTCCAG 
CTGCAGATTA GCGAGATGTC CGATCTGAGG TGCCTTTTTG TCCTGCTGCT TAGGCGCTTC 
AATCCCAGCA TCCATTCCAA GCAGTATCTC CAGGATCTGG TGGTTACCAA TCACATCCTC 
CTCCTCATTC TGGACAGTTC GGCCAAACTG GGTGGATGTC AAACCATTCG CCTGTCGGAG 
CACATTACAC AGTTTGCCAC GCTGGAGGTG ATGCACTACT ATGGCATCCT CTTGGAGGAC 
TTCAACAACA ACGGAGAGTT TGTCAATGAC TGCATCTTCA CCATGATGCA TCACATAGGT 
GGCGATCTGG GCCAGATTGG GGTTCTATTC CAACCAATTA TTTTGAAAAC CTATTCAAGA 
ATTTGGGAAG CGGACTATGA ACTGTGCGAT GACTGGTCTG ATCTTATCGA GTATGTGATT 
CACAAGTTCA TGAATACTCC TCCGAAGTCG CCACTCACCA TTCCCACAAC TTCCTTAACG 
GAAATGACCA AGGAACACAA CCAGGAGCAT ACCGTTTGCT CATGGTCGCA GGAGGAAATG 
GATACACTGT ATTGGTATTA TGTGCAGAGC AAGAAGAACA ACGATATTGT GGGAAAGATA 
GTTAAGCTCT TCAGCAACAA CGGCAACAAG CTGAAAACCC GGATTTCTAT TATCCAACAA 
CTTTTGCAAC AAGACATTAT TACCCTGTTG GAGTACGATG ATCTGATGAA GTTCGAGGAT 
GCGGAGTATC AGAGAACTTT GCTGACCACT CCCACTTCCG CAACCACAGA GTCTGGAATT 
GAGATTAAGG AGTGCGCCTA CGGCAAACCC TCAGATGATG TGCAGATCCT GCTGGACCTG 
ATCATCAAGG AAAACAAAGC GCAGCATTTG TTATGGCTGC AGAGGATCCT CATCGAGTGC 
TGCTTCGTCA AGCTGACCCT GCGGAGTGGT CTCAAGGTTC CGGAAGGCGA TCACATCATG 
GAGCCGGTGG CCTATCACTG CATCTGCAAG CAGAAGTCCA TTCCGGTGGT GCAGTGGAAC 
AACGAGCAAT CCACTACGAT GCTGTACCAG CCCTTTGTGC TGCTGCTCCA CAAACTGGGC 
ATTCAGCTGC CGGCGGACGC GGGCTCGATC TTCGCCAGAA TTCCGGACTA CTGGACACCG 
GAGACCATGT ACGGACTGGC CAAGAAGCTG GGACCGCTGG ACAAACTCAA CCTAAAGTTC 
GACACCAGCG AACTGGAGGA TGCGACGGCG TCGAGTCCGT CGCGGTACCA CCACACCGGA 
CCCCGCAACT CGCTCAGCTC GGTGAGCAGC CTGGACGTGG ACCTCGGCGA GCCCGAGGAG 
CTGGCCCTCA TACCCGAGGT GGATGCGGCC GTGGAGAAGG CACACGCCAT GGCGTCCACG 
CCATCGCCCA GCGAGATGTT CGCGGTGCCC AAGACGAAGC ACTGCAACTC GATCATCAGA 
TACACACCCG ATCCCACGCC TCCAGTGCCC AACTGGCTGC AGTTGGTCAT GCGCAGCAAA 
TGCAATCAGC GCACAGGTCC GGCTGGTGAT CCCAGCGATT GCGTGGGCTC CTCGTCGACA 
ACAGTGGACG ATGAGGGATT GGGCAAGTCC ATCAGTGCAG CCACTTCGCA GGCGGCGAGC 
ACCTCCATGA GCACGGTTAA TCCCACAACC ACTTTGAGCC TCAATATGCT GAACACCTTC 
ATGGGAAGCC ACAACGAGAA CAGCAGCAGT TCCGGATGCG GAGGCACCGT GTCCTCCCTG 
TCCATGGTGG CTCTGATGAG CACTGGCGCG GTCGGCGGAG GAGGCAACAC CTCTGGACTG 
GACATGGATG TGGACGCCTC CATGAAGTCG TCGTTCGAGC GACTGGAGGT CAACGGATCG 
CACTTCTCGC GGGCCAACAA CTTGGACCAG GAGTACAGTG CTATGGTGGC ATCTGTGTAC 
GAAAAGGAGA AGGAATTAAA CAGCGACAAT GTCTCTTTGG CCTCGGACCT GACCAGAATG 
TATGTGAGCG ATGAGGACGA TCGACTTGAG CGAACCGAGA TCCGGGTGCC CCACTATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016023299.1
CDS	116..4318
Translation	MDWLLATPQLYSAFSSLGCLEGDTYVVNPNALAILEEINYKLTYEDQTLRTFRRAIGFGQNVRSDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDVMYRTDVGRHTIFELNKLLYTSKEAFTEARSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHCVMPMMQSMPHGISMQNTILWNLFIQSIDKLLLYLMTCPQRAFWGVTMVQLIALIYKDQHVSTLQKLLSLWFEASLSESSEDNESNTSPPKQGSGDSSPMLTSDPTSDSSDNGSNGRGMGGGMREGTAATLQEVSRKGQEYQNAMARVPADKPDGSEEASDMTGNDSEQPGSPEQSQPAGESMDDGDYEDQRHSQLNEHGEEDEDEDEVEEEEYLQLGPASEPLNLTQQPADKVNNTTSPTSSPPQGCLGTEPFKPPPPLPVRASTSAHAQMQKFNESSYASHVSAVKLGQKSPHAGQLQLTKGKCCPQKRECPSSQSELSDCGYGTQVENQESISTSSNDDDGPQGKPQHQKPPCNTKPRNKPRTIMSPMDKKELRRKKLVKRSKSSLINMKGLVQHTPTDDDISNLLKEFTVDFLLKGYSYLVEELHMQLLSNANKKVPIDTSHFFWLVTYFLKFAAQLELDMEHIDTILTYDVLSYLTYEGVSLCEQLELNARQEGSDLKPYLRRMHLVVTAIREFLQAIDTYNKVTHLNEDDKAHLRQLQLQISEMSDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDSSAKLGGCQTIRLSEHITQFATLEVMHYYGILLEDFNNNGEFVNDCIFTMMHHIGGDLGQIGVLFQPIILKTYSRIWEADYELCDDWSDLIEYVIHKFMNTPPKSPLTIPTTSLTEMTKEHNQEHTVCSWSQEEMDTLYWYYVQSKKNNDIVGKIVKLFSNNGNKLKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLTTPTSATTESGIEIKECAYGKPSDDVQILLDLIIKENKAQHLLWLQRILIECCFVKLTLRSGLKVPEGDHIMEPVAYHCICKQKSIPVVQWNNEQSTTMLYQPFVLLLHKLGIQLPADAGSIFARIPDYWTPETMYGLAKKLGPLDKLNLKFDTSELEDATASSPSRYHHTGPRNSLSSVSSLDVDLGEPEELALIPEVDAAVEKAHAMASTPSPSEMFAVPKTKHCNSIIRYTPDPTPPVPNWLQLVMRSKCNQRTGPAGDPSDCVGSSSTTVDDEGLGKSISAATSQAASTSMSTVNPTTTLSLNMLNTFMGSHNENSSSSGCGGTVSSLSMVALMSTGAVGGGGNTSGLDMDVDASMKSSFERLEVNGSHFSRANNLDQEYSAMVASVYEKEKELNSDNVSLASDLTRMYVSDEDDRLERTEIRVPHYH

Target ORF information:

RefSeq Version	XM_016179065.1
Organism	Drosophila simulans
Definition	Drosophila simulans tim, transcript variant F (Dsim\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016179065.1

ORF Insert Sequence:

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAACA AGAAGGTGCC CATTGACACA 
TCGCACTTCT TCTGGCTGGT CACCTACTTC CTGAAGTTTG CCGCCCAACT GGAGCTGGAC 
ATGGAGCACA TCGACACCAT TCTCACCTAC GATGTTTTGA GCTACTTGAC CTATGAGGGT 
GTTTCCCTTT GTGAACAACT GGAACTGAAT GCCCGACAGG AGGGCAGTGA CCTGAAGCCC 
TATCTGAGGC GGATGCACTT GGTGGTGACG GCCATCCGGG AGTTCCTCCA GGCCATCGAC 
ACATACAACA AAGTGACCCA TCTGAACGAG GACGACAAGG CCCATTTGAG GCAGCTCCAG 
CTGCAGATTA GCGAGATGTC CGATCTGAGG TGCCTTTTTG TCCTGCTGCT TAGGCGCTTC 
AATCCCAGCA TCCATTCCAA GCAGTATCTC CAGGATCTGG TGGTTACCAA TCACATCCTC 
CTCCTCATTC TGGACAGTTC GGCCAAACTG GGTGGATGTC AAACCATTCG CCTGTCGGAG 
CACATTACAC AGTTTGCCAC GCTGGAGGTG ATGCACTACT ATGGCATCCT CTTGGAGGAC 
TTCAACAACA ACGGAGAGTT TGTCAATGAC TGCATCTTCA CCATGATGCA TCACATAGGT 
GGCGATCTGG GCCAGATTGG GGTTCTATTC CAACCAATTA TTTTGAAAAC CTATTCAAGA 
ATTTGGGAAG CGGACTATGA ACTGTGCGAT GACTGGTCTG ATCTTATCGA GTATGTGATT 
CACAAGTTCA TGAATACTCC TCCGAAGTCG CCACTCACCA TTCCCACAAC TTCCTTAACG 
GAAATGACCA AGGAACACAA CCAGGAGCAT ACCGTTTGCT CATGGTCGCA GGAGGAAATG 
GATACACTGT ATTGGTATTA TGTGCAGAGC AAGAAGAACA ACGATATTGT GGGAAAGATA 
GTTAAGCTCT TCAGCAACAA CGGCAACAAG CTGAAAACCC GGATTTCTAT TATCCAACAA 
CTTTTGCAAC AAGACATTAT TACCCTGTTG GAGTACGATG ATCTGATGAA GTTCGAGGAT 
GCGGAGTATC AGAGAACTTT GCTGACCACT CCCACTTCCG CAACCACAGA GTCTGGAATT 
GAGATTAAGG AGTGCGCCTA CGGCAAACCC TCAGATGATG TGCAGATCCT GCTGGACCTG 
ATCATCAAGG AAAACAAAGC GCAGCATTTG TTATGGCTGC AGAGGATCCT CATCGAGTGC 
TGCTTCGTCA AGCTGACCCT GCGGAGTGGT CTCAAGGTTC CGGAAGGCGA TCACATCATG 
GAGCCGGTGG CCTATCACTG CATCTGCAAG CAGAAGTCCA TTCCGGTGGT GCAGTGGAAC 
AACGAGCAAT CCACTACGAT GCTGTACCAG CCCTTTGTGC TGCTGCTCCA CAAACTGGGC 
ATTCAGCTGC CGGCGGACGC GGGCTCGATC TTCGCCAGAA TTCCGGACTA CTGGACACCG 
GAGACCATGT ACGGACTGGC CAAGAAGCTG GGACCGCTGG ACAAACTCAA CCTAAAGTTC 
GACACCAGCG AACTGGAGGA TGCGACGGCG TCGAGTCCGT CGCGGTACCA CCACACCGGA 
CCCCGCAACT CGCTCAGCTC GGTGAGCAGC CTGGACGTGG ACCTCGGCGA GCCCGAGGAG 
CTGGCCCTCA TACCCGAGGT GGATGCGGCC GTGGAGAAGG CACACGCCAT GGCGTCCACG 
CCATCGCCCA GCGAGATGTT CGCGGTGCCC AAGACGAAGC ACTGCAACTC GATCATCAGA 
TACACACCCG ATCCCACGCC TCCAGTGCCC AACTGGCTGC AGTTGGTCAT GCGCAGCAAA 
TGCAATCAGC GCACAGGTCC GGCTGGTGAT CCCAGCGATT GCGTGGGCTC CTCGTCGACA 
ACAGTGGACG ATGAGGGATT GGGCAAGTCC ATCAGTGCAG CCACTTCGCA GGCGGCGAGC 
ACCTCCATGA GCACGGTTAA TCCCACAACC ACTTTGAGCC TCAATATGCT GAACACCTTC 
ATGGGAAGCC ACAACGAGAA CAGCAGCAGT TCCGGATGCG GAGGCACCGT GTCCTCCCTG 
TCCATGGTGG CTCTGATGAG CACTGGCGCG GTCGGCGGAG GAGGCAACAC CTCTGGACTG 
GACATGGATG TGGACGCCTC CATGAAGTCG TCGTTCGAGC GACTGGAGGT CAACGGATCG 
CACTTCTCGC GGGCCAACAA CTTGGACCAG GAGTACAGTG CTATGGTGGC ATCTGTGTAC 
GAAAAGGAGA AGGAATTAAA CAGCGACAAT GTCTCTTTGG CCTCGGACCT GACCAGAATG 
TATGTGAGCG ATGAGGACGA TCGACTTGAG CGAACCGAGA TCCGGGTGCC CCACTATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\tim ( XM_016179063.1 ) cDNA ORF clone, Drosophila simulans -> XP_016023297.1 Drosophila simulans tim, transcript variant D (Dsim\tim), mRNA.

Price & Availability↑

CloneID	ODg18787
Clone ID Related Accession (Same CDS sequence)	XM_016179065.1 , XM_016179063.1 , XM_016179064.1
Accession Version	XM_016179063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	tim, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAACA AGAAGGTGCC CATTGACACA 
TCGCACTTCT TCTGGCTGGT CACCTACTTC CTGAAGTTTG CCGCCCAACT GGAGCTGGAC 
ATGGAGCACA TCGACACCAT TCTCACCTAC GATGTTTTGA GCTACTTGAC CTATGAGGGT 
GTTTCCCTTT GTGAACAACT GGAACTGAAT GCCCGACAGG AGGGCAGTGA CCTGAAGCCC 
TATCTGAGGC GGATGCACTT GGTGGTGACG GCCATCCGGG AGTTCCTCCA GGCCATCGAC 
ACATACAACA AAGTGACCCA TCTGAACGAG GACGACAAGG CCCATTTGAG GCAGCTCCAG 
CTGCAGATTA GCGAGATGTC CGATCTGAGG TGCCTTTTTG TCCTGCTGCT TAGGCGCTTC 
AATCCCAGCA TCCATTCCAA GCAGTATCTC CAGGATCTGG TGGTTACCAA TCACATCCTC 
CTCCTCATTC TGGACAGTTC GGCCAAACTG GGTGGATGTC AAACCATTCG CCTGTCGGAG 
CACATTACAC AGTTTGCCAC GCTGGAGGTG ATGCACTACT ATGGCATCCT CTTGGAGGAC 
TTCAACAACA ACGGAGAGTT TGTCAATGAC TGCATCTTCA CCATGATGCA TCACATAGGT 
GGCGATCTGG GCCAGATTGG GGTTCTATTC CAACCAATTA TTTTGAAAAC CTATTCAAGA 
ATTTGGGAAG CGGACTATGA ACTGTGCGAT GACTGGTCTG ATCTTATCGA GTATGTGATT 
CACAAGTTCA TGAATACTCC TCCGAAGTCG CCACTCACCA TTCCCACAAC TTCCTTAACG 
GAAATGACCA AGGAACACAA CCAGGAGCAT ACCGTTTGCT CATGGTCGCA GGAGGAAATG 
GATACACTGT ATTGGTATTA TGTGCAGAGC AAGAAGAACA ACGATATTGT GGGAAAGATA 
GTTAAGCTCT TCAGCAACAA CGGCAACAAG CTGAAAACCC GGATTTCTAT TATCCAACAA 
CTTTTGCAAC AAGACATTAT TACCCTGTTG GAGTACGATG ATCTGATGAA GTTCGAGGAT 
GCGGAGTATC AGAGAACTTT GCTGACCACT CCCACTTCCG CAACCACAGA GTCTGGAATT 
GAGATTAAGG AGTGCGCCTA CGGCAAACCC TCAGATGATG TGCAGATCCT GCTGGACCTG 
ATCATCAAGG AAAACAAAGC GCAGCATTTG TTATGGCTGC AGAGGATCCT CATCGAGTGC 
TGCTTCGTCA AGCTGACCCT GCGGAGTGGT CTCAAGGTTC CGGAAGGCGA TCACATCATG 
GAGCCGGTGG CCTATCACTG CATCTGCAAG CAGAAGTCCA TTCCGGTGGT GCAGTGGAAC 
AACGAGCAAT CCACTACGAT GCTGTACCAG CCCTTTGTGC TGCTGCTCCA CAAACTGGGC 
ATTCAGCTGC CGGCGGACGC GGGCTCGATC TTCGCCAGAA TTCCGGACTA CTGGACACCG 
GAGACCATGT ACGGACTGGC CAAGAAGCTG GGACCGCTGG ACAAACTCAA CCTAAAGTTC 
GACACCAGCG AACTGGAGGA TGCGACGGCG TCGAGTCCGT CGCGGTACCA CCACACCGGA 
CCCCGCAACT CGCTCAGCTC GGTGAGCAGC CTGGACGTGG ACCTCGGCGA GCCCGAGGAG 
CTGGCCCTCA TACCCGAGGT GGATGCGGCC GTGGAGAAGG CACACGCCAT GGCGTCCACG 
CCATCGCCCA GCGAGATGTT CGCGGTGCCC AAGACGAAGC ACTGCAACTC GATCATCAGA 
TACACACCCG ATCCCACGCC TCCAGTGCCC AACTGGCTGC AGTTGGTCAT GCGCAGCAAA 
TGCAATCAGC GCACAGGTCC GGCTGGTGAT CCCAGCGATT GCGTGGGCTC CTCGTCGACA 
ACAGTGGACG ATGAGGGATT GGGCAAGTCC ATCAGTGCAG CCACTTCGCA GGCGGCGAGC 
ACCTCCATGA GCACGGTTAA TCCCACAACC ACTTTGAGCC TCAATATGCT GAACACCTTC 
ATGGGAAGCC ACAACGAGAA CAGCAGCAGT TCCGGATGCG GAGGCACCGT GTCCTCCCTG 
TCCATGGTGG CTCTGATGAG CACTGGCGCG GTCGGCGGAG GAGGCAACAC CTCTGGACTG 
GACATGGATG TGGACGCCTC CATGAAGTCG TCGTTCGAGC GACTGGAGGT CAACGGATCG 
CACTTCTCGC GGGCCAACAA CTTGGACCAG GAGTACAGTG CTATGGTGGC ATCTGTGTAC 
GAAAAGGAGA AGGAATTAAA CAGCGACAAT GTCTCTTTGG CCTCGGACCT GACCAGAATG 
TATGTGAGCG ATGAGGACGA TCGACTTGAG CGAACCGAGA TCCGGGTGCC CCACTATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016023297.1
CDS	178..4380
Translation	MDWLLATPQLYSAFSSLGCLEGDTYVVNPNALAILEEINYKLTYEDQTLRTFRRAIGFGQNVRSDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDVMYRTDVGRHTIFELNKLLYTSKEAFTEARSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHCVMPMMQSMPHGISMQNTILWNLFIQSIDKLLLYLMTCPQRAFWGVTMVQLIALIYKDQHVSTLQKLLSLWFEASLSESSEDNESNTSPPKQGSGDSSPMLTSDPTSDSSDNGSNGRGMGGGMREGTAATLQEVSRKGQEYQNAMARVPADKPDGSEEASDMTGNDSEQPGSPEQSQPAGESMDDGDYEDQRHSQLNEHGEEDEDEDEVEEEEYLQLGPASEPLNLTQQPADKVNNTTSPTSSPPQGCLGTEPFKPPPPLPVRASTSAHAQMQKFNESSYASHVSAVKLGQKSPHAGQLQLTKGKCCPQKRECPSSQSELSDCGYGTQVENQESISTSSNDDDGPQGKPQHQKPPCNTKPRNKPRTIMSPMDKKELRRKKLVKRSKSSLINMKGLVQHTPTDDDISNLLKEFTVDFLLKGYSYLVEELHMQLLSNANKKVPIDTSHFFWLVTYFLKFAAQLELDMEHIDTILTYDVLSYLTYEGVSLCEQLELNARQEGSDLKPYLRRMHLVVTAIREFLQAIDTYNKVTHLNEDDKAHLRQLQLQISEMSDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDSSAKLGGCQTIRLSEHITQFATLEVMHYYGILLEDFNNNGEFVNDCIFTMMHHIGGDLGQIGVLFQPIILKTYSRIWEADYELCDDWSDLIEYVIHKFMNTPPKSPLTIPTTSLTEMTKEHNQEHTVCSWSQEEMDTLYWYYVQSKKNNDIVGKIVKLFSNNGNKLKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLTTPTSATTESGIEIKECAYGKPSDDVQILLDLIIKENKAQHLLWLQRILIECCFVKLTLRSGLKVPEGDHIMEPVAYHCICKQKSIPVVQWNNEQSTTMLYQPFVLLLHKLGIQLPADAGSIFARIPDYWTPETMYGLAKKLGPLDKLNLKFDTSELEDATASSPSRYHHTGPRNSLSSVSSLDVDLGEPEELALIPEVDAAVEKAHAMASTPSPSEMFAVPKTKHCNSIIRYTPDPTPPVPNWLQLVMRSKCNQRTGPAGDPSDCVGSSSTTVDDEGLGKSISAATSQAASTSMSTVNPTTTLSLNMLNTFMGSHNENSSSSGCGGTVSSLSMVALMSTGAVGGGGNTSGLDMDVDASMKSSFERLEVNGSHFSRANNLDQEYSAMVASVYEKEKELNSDNVSLASDLTRMYVSDEDDRLERTEIRVPHYH

Target ORF information:

RefSeq Version	XM_016179063.1
Organism	Drosophila simulans
Definition	Drosophila simulans tim, transcript variant D (Dsim\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016179063.1

ORF Insert Sequence:

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAACA AGAAGGTGCC CATTGACACA 
TCGCACTTCT TCTGGCTGGT CACCTACTTC CTGAAGTTTG CCGCCCAACT GGAGCTGGAC 
ATGGAGCACA TCGACACCAT TCTCACCTAC GATGTTTTGA GCTACTTGAC CTATGAGGGT 
GTTTCCCTTT GTGAACAACT GGAACTGAAT GCCCGACAGG AGGGCAGTGA CCTGAAGCCC 
TATCTGAGGC GGATGCACTT GGTGGTGACG GCCATCCGGG AGTTCCTCCA GGCCATCGAC 
ACATACAACA AAGTGACCCA TCTGAACGAG GACGACAAGG CCCATTTGAG GCAGCTCCAG 
CTGCAGATTA GCGAGATGTC CGATCTGAGG TGCCTTTTTG TCCTGCTGCT TAGGCGCTTC 
AATCCCAGCA TCCATTCCAA GCAGTATCTC CAGGATCTGG TGGTTACCAA TCACATCCTC 
CTCCTCATTC TGGACAGTTC GGCCAAACTG GGTGGATGTC AAACCATTCG CCTGTCGGAG 
CACATTACAC AGTTTGCCAC GCTGGAGGTG ATGCACTACT ATGGCATCCT CTTGGAGGAC 
TTCAACAACA ACGGAGAGTT TGTCAATGAC TGCATCTTCA CCATGATGCA TCACATAGGT 
GGCGATCTGG GCCAGATTGG GGTTCTATTC CAACCAATTA TTTTGAAAAC CTATTCAAGA 
ATTTGGGAAG CGGACTATGA ACTGTGCGAT GACTGGTCTG ATCTTATCGA GTATGTGATT 
CACAAGTTCA TGAATACTCC TCCGAAGTCG CCACTCACCA TTCCCACAAC TTCCTTAACG 
GAAATGACCA AGGAACACAA CCAGGAGCAT ACCGTTTGCT CATGGTCGCA GGAGGAAATG 
GATACACTGT ATTGGTATTA TGTGCAGAGC AAGAAGAACA ACGATATTGT GGGAAAGATA 
GTTAAGCTCT TCAGCAACAA CGGCAACAAG CTGAAAACCC GGATTTCTAT TATCCAACAA 
CTTTTGCAAC AAGACATTAT TACCCTGTTG GAGTACGATG ATCTGATGAA GTTCGAGGAT 
GCGGAGTATC AGAGAACTTT GCTGACCACT CCCACTTCCG CAACCACAGA GTCTGGAATT 
GAGATTAAGG AGTGCGCCTA CGGCAAACCC TCAGATGATG TGCAGATCCT GCTGGACCTG 
ATCATCAAGG AAAACAAAGC GCAGCATTTG TTATGGCTGC AGAGGATCCT CATCGAGTGC 
TGCTTCGTCA AGCTGACCCT GCGGAGTGGT CTCAAGGTTC CGGAAGGCGA TCACATCATG 
GAGCCGGTGG CCTATCACTG CATCTGCAAG CAGAAGTCCA TTCCGGTGGT GCAGTGGAAC 
AACGAGCAAT CCACTACGAT GCTGTACCAG CCCTTTGTGC TGCTGCTCCA CAAACTGGGC 
ATTCAGCTGC CGGCGGACGC GGGCTCGATC TTCGCCAGAA TTCCGGACTA CTGGACACCG 
GAGACCATGT ACGGACTGGC CAAGAAGCTG GGACCGCTGG ACAAACTCAA CCTAAAGTTC 
GACACCAGCG AACTGGAGGA TGCGACGGCG TCGAGTCCGT CGCGGTACCA CCACACCGGA 
CCCCGCAACT CGCTCAGCTC GGTGAGCAGC CTGGACGTGG ACCTCGGCGA GCCCGAGGAG 
CTGGCCCTCA TACCCGAGGT GGATGCGGCC GTGGAGAAGG CACACGCCAT GGCGTCCACG 
CCATCGCCCA GCGAGATGTT CGCGGTGCCC AAGACGAAGC ACTGCAACTC GATCATCAGA 
TACACACCCG ATCCCACGCC TCCAGTGCCC AACTGGCTGC AGTTGGTCAT GCGCAGCAAA 
TGCAATCAGC GCACAGGTCC GGCTGGTGAT CCCAGCGATT GCGTGGGCTC CTCGTCGACA 
ACAGTGGACG ATGAGGGATT GGGCAAGTCC ATCAGTGCAG CCACTTCGCA GGCGGCGAGC 
ACCTCCATGA GCACGGTTAA TCCCACAACC ACTTTGAGCC TCAATATGCT GAACACCTTC 
ATGGGAAGCC ACAACGAGAA CAGCAGCAGT TCCGGATGCG GAGGCACCGT GTCCTCCCTG 
TCCATGGTGG CTCTGATGAG CACTGGCGCG GTCGGCGGAG GAGGCAACAC CTCTGGACTG 
GACATGGATG TGGACGCCTC CATGAAGTCG TCGTTCGAGC GACTGGAGGT CAACGGATCG 
CACTTCTCGC GGGCCAACAA CTTGGACCAG GAGTACAGTG CTATGGTGGC ATCTGTGTAC 
GAAAAGGAGA AGGAATTAAA CAGCGACAAT GTCTCTTTGG CCTCGGACCT GACCAGAATG 
TATGTGAGCG ATGAGGACGA TCGACTTGAG CGAACCGAGA TCCGGGTGCC CCACTATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\tim ( XM_016179064.1 ) cDNA ORF clone, Drosophila simulans -> XP_016023298.1 Drosophila simulans tim, transcript variant E (Dsim\tim), mRNA.

Price & Availability↑

CloneID	ODg18787
Clone ID Related Accession (Same CDS sequence)	XM_016179065.1 , XM_016179063.1 , XM_016179064.1
Accession Version	XM_016179064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	tim, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479533). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAACA AGAAGGTGCC CATTGACACA 
TCGCACTTCT TCTGGCTGGT CACCTACTTC CTGAAGTTTG CCGCCCAACT GGAGCTGGAC 
ATGGAGCACA TCGACACCAT TCTCACCTAC GATGTTTTGA GCTACTTGAC CTATGAGGGT 
GTTTCCCTTT GTGAACAACT GGAACTGAAT GCCCGACAGG AGGGCAGTGA CCTGAAGCCC 
TATCTGAGGC GGATGCACTT GGTGGTGACG GCCATCCGGG AGTTCCTCCA GGCCATCGAC 
ACATACAACA AAGTGACCCA TCTGAACGAG GACGACAAGG CCCATTTGAG GCAGCTCCAG 
CTGCAGATTA GCGAGATGTC CGATCTGAGG TGCCTTTTTG TCCTGCTGCT TAGGCGCTTC 
AATCCCAGCA TCCATTCCAA GCAGTATCTC CAGGATCTGG TGGTTACCAA TCACATCCTC 
CTCCTCATTC TGGACAGTTC GGCCAAACTG GGTGGATGTC AAACCATTCG CCTGTCGGAG 
CACATTACAC AGTTTGCCAC GCTGGAGGTG ATGCACTACT ATGGCATCCT CTTGGAGGAC 
TTCAACAACA ACGGAGAGTT TGTCAATGAC TGCATCTTCA CCATGATGCA TCACATAGGT 
GGCGATCTGG GCCAGATTGG GGTTCTATTC CAACCAATTA TTTTGAAAAC CTATTCAAGA 
ATTTGGGAAG CGGACTATGA ACTGTGCGAT GACTGGTCTG ATCTTATCGA GTATGTGATT 
CACAAGTTCA TGAATACTCC TCCGAAGTCG CCACTCACCA TTCCCACAAC TTCCTTAACG 
GAAATGACCA AGGAACACAA CCAGGAGCAT ACCGTTTGCT CATGGTCGCA GGAGGAAATG 
GATACACTGT ATTGGTATTA TGTGCAGAGC AAGAAGAACA ACGATATTGT GGGAAAGATA 
GTTAAGCTCT TCAGCAACAA CGGCAACAAG CTGAAAACCC GGATTTCTAT TATCCAACAA 
CTTTTGCAAC AAGACATTAT TACCCTGTTG GAGTACGATG ATCTGATGAA GTTCGAGGAT 
GCGGAGTATC AGAGAACTTT GCTGACCACT CCCACTTCCG CAACCACAGA GTCTGGAATT 
GAGATTAAGG AGTGCGCCTA CGGCAAACCC TCAGATGATG TGCAGATCCT GCTGGACCTG 
ATCATCAAGG AAAACAAAGC GCAGCATTTG TTATGGCTGC AGAGGATCCT CATCGAGTGC 
TGCTTCGTCA AGCTGACCCT GCGGAGTGGT CTCAAGGTTC CGGAAGGCGA TCACATCATG 
GAGCCGGTGG CCTATCACTG CATCTGCAAG CAGAAGTCCA TTCCGGTGGT GCAGTGGAAC 
AACGAGCAAT CCACTACGAT GCTGTACCAG CCCTTTGTGC TGCTGCTCCA CAAACTGGGC 
ATTCAGCTGC CGGCGGACGC GGGCTCGATC TTCGCCAGAA TTCCGGACTA CTGGACACCG 
GAGACCATGT ACGGACTGGC CAAGAAGCTG GGACCGCTGG ACAAACTCAA CCTAAAGTTC 
GACACCAGCG AACTGGAGGA TGCGACGGCG TCGAGTCCGT CGCGGTACCA CCACACCGGA 
CCCCGCAACT CGCTCAGCTC GGTGAGCAGC CTGGACGTGG ACCTCGGCGA GCCCGAGGAG 
CTGGCCCTCA TACCCGAGGT GGATGCGGCC GTGGAGAAGG CACACGCCAT GGCGTCCACG 
CCATCGCCCA GCGAGATGTT CGCGGTGCCC AAGACGAAGC ACTGCAACTC GATCATCAGA 
TACACACCCG ATCCCACGCC TCCAGTGCCC AACTGGCTGC AGTTGGTCAT GCGCAGCAAA 
TGCAATCAGC GCACAGGTCC GGCTGGTGAT CCCAGCGATT GCGTGGGCTC CTCGTCGACA 
ACAGTGGACG ATGAGGGATT GGGCAAGTCC ATCAGTGCAG CCACTTCGCA GGCGGCGAGC 
ACCTCCATGA GCACGGTTAA TCCCACAACC ACTTTGAGCC TCAATATGCT GAACACCTTC 
ATGGGAAGCC ACAACGAGAA CAGCAGCAGT TCCGGATGCG GAGGCACCGT GTCCTCCCTG 
TCCATGGTGG CTCTGATGAG CACTGGCGCG GTCGGCGGAG GAGGCAACAC CTCTGGACTG 
GACATGGATG TGGACGCCTC CATGAAGTCG TCGTTCGAGC GACTGGAGGT CAACGGATCG 
CACTTCTCGC GGGCCAACAA CTTGGACCAG GAGTACAGTG CTATGGTGGC ATCTGTGTAC 
GAAAAGGAGA AGGAATTAAA CAGCGACAAT GTCTCTTTGG CCTCGGACCT GACCAGAATG 
TATGTGAGCG ATGAGGACGA TCGACTTGAG CGAACCGAGA TCCGGGTGCC CCACTATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016023298.1
CDS	118..4320
Translation	MDWLLATPQLYSAFSSLGCLEGDTYVVNPNALAILEEINYKLTYEDQTLRTFRRAIGFGQNVRSDLIPLLENAKDDAVLESVIRILVNLTVPVECLFSVDVMYRTDVGRHTIFELNKLLYTSKEAFTEARSTKSVVEYMKHILESDPKLSPHKCDQINNCLLLLRNILHIPETHAHCVMPMMQSMPHGISMQNTILWNLFIQSIDKLLLYLMTCPQRAFWGVTMVQLIALIYKDQHVSTLQKLLSLWFEASLSESSEDNESNTSPPKQGSGDSSPMLTSDPTSDSSDNGSNGRGMGGGMREGTAATLQEVSRKGQEYQNAMARVPADKPDGSEEASDMTGNDSEQPGSPEQSQPAGESMDDGDYEDQRHSQLNEHGEEDEDEDEVEEEEYLQLGPASEPLNLTQQPADKVNNTTSPTSSPPQGCLGTEPFKPPPPLPVRASTSAHAQMQKFNESSYASHVSAVKLGQKSPHAGQLQLTKGKCCPQKRECPSSQSELSDCGYGTQVENQESISTSSNDDDGPQGKPQHQKPPCNTKPRNKPRTIMSPMDKKELRRKKLVKRSKSSLINMKGLVQHTPTDDDISNLLKEFTVDFLLKGYSYLVEELHMQLLSNANKKVPIDTSHFFWLVTYFLKFAAQLELDMEHIDTILTYDVLSYLTYEGVSLCEQLELNARQEGSDLKPYLRRMHLVVTAIREFLQAIDTYNKVTHLNEDDKAHLRQLQLQISEMSDLRCLFVLLLRRFNPSIHSKQYLQDLVVTNHILLLILDSSAKLGGCQTIRLSEHITQFATLEVMHYYGILLEDFNNNGEFVNDCIFTMMHHIGGDLGQIGVLFQPIILKTYSRIWEADYELCDDWSDLIEYVIHKFMNTPPKSPLTIPTTSLTEMTKEHNQEHTVCSWSQEEMDTLYWYYVQSKKNNDIVGKIVKLFSNNGNKLKTRISIIQQLLQQDIITLLEYDDLMKFEDAEYQRTLLTTPTSATTESGIEIKECAYGKPSDDVQILLDLIIKENKAQHLLWLQRILIECCFVKLTLRSGLKVPEGDHIMEPVAYHCICKQKSIPVVQWNNEQSTTMLYQPFVLLLHKLGIQLPADAGSIFARIPDYWTPETMYGLAKKLGPLDKLNLKFDTSELEDATASSPSRYHHTGPRNSLSSVSSLDVDLGEPEELALIPEVDAAVEKAHAMASTPSPSEMFAVPKTKHCNSIIRYTPDPTPPVPNWLQLVMRSKCNQRTGPAGDPSDCVGSSSTTVDDEGLGKSISAATSQAASTSMSTVNPTTTLSLNMLNTFMGSHNENSSSSGCGGTVSSLSMVALMSTGAVGGGGNTSGLDMDVDASMKSSFERLEVNGSHFSRANNLDQEYSAMVASVYEKEKELNSDNVSLASDLTRMYVSDEDDRLERTEIRVPHYH

Target ORF information:

RefSeq Version	XM_016179064.1
Organism	Drosophila simulans
Definition	Drosophila simulans tim, transcript variant E (Dsim\tim), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016179064.1

ORF Insert Sequence:

ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
AAGCTCACCT ATGAGGACCA AACGCTGCGC ACCTTTCGAC GGGCCATTGG ATTCGGCCAG 
AATGTGAGGT CAGATCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
TCGGTCATCC GGATACTGGT CAACCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
CACATCCTGG AGTCGGACCC CAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
CTGCTGCTTC TGAGGAATAT CCTGCACATT CCGGAGACGC ATGCCCATTG CGTGATGCCC 
ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAACCTTTTC 
ATCCAGAGCA TAGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
GGAGTGACCA TGGTGCAACT GATTGCTCTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
CAAAAACTAC TAAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
AGTAATACCT CGCCCCCCAA ACAGGGAAGT GGCGATTCCA GCCCCATGCT GACTTCCGAT 
CCCACCTCCG ATTCCTCGGA CAACGGCAGC AATGGCCGTG GCATGGGTGG TGGCATGCGG 
GAGGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAAG GTCAGGAATA CCAGAACGCC 
ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
AACGACAGCG AGCAGCCAGG ATCGCCGGAG CAATCACAGC CCGCCGGCGA GTCCATGGAC 
GATGGAGATT ACGAGGACCA GAGACACAGC CAGCTGAACG AGCACGGCGA GGAGGATGAA 
GATGAGGACG AAGTGGAGGA AGAGGAGTAC CTACAATTGG GACCAGCCTC GGAGCCCCTA 
AACTTAACCC AACAACCAGC TGACAAGGTC AACAACACTA CCAGCCCAAC GTCCAGCCCG 
CCACAGGGCT GCCTGGGCAC AGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
TCTGCGGTTA AATTGGGCCA AAAGTCCCCC CATGCCGGAC AACTTCAGCT GACCAAGGGC 
AAGTGTTGTC CACAGAAACG GGAATGCCCC TCCTCGCAGT CTGAGCTATC GGATTGCGGT 
TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGACGATGGA 
CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTAAGAC GCAAAAAACT GGTCAAGCGC 
AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTTCAGC ACACCCCCAC TGATGATGAC 
ATCTCTAATC TGCTAAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
GTGGAGGAAT TGCACATGCA ACTGCTTTCC AATGCGAACA AGAAGGTGCC CATTGACACA 
TCGCACTTCT TCTGGCTGGT CACCTACTTC CTGAAGTTTG CCGCCCAACT GGAGCTGGAC 
ATGGAGCACA TCGACACCAT TCTCACCTAC GATGTTTTGA GCTACTTGAC CTATGAGGGT 
GTTTCCCTTT GTGAACAACT GGAACTGAAT GCCCGACAGG AGGGCAGTGA CCTGAAGCCC 
TATCTGAGGC GGATGCACTT GGTGGTGACG GCCATCCGGG AGTTCCTCCA GGCCATCGAC 
ACATACAACA AAGTGACCCA TCTGAACGAG GACGACAAGG CCCATTTGAG GCAGCTCCAG 
CTGCAGATTA GCGAGATGTC CGATCTGAGG TGCCTTTTTG TCCTGCTGCT TAGGCGCTTC 
AATCCCAGCA TCCATTCCAA GCAGTATCTC CAGGATCTGG TGGTTACCAA TCACATCCTC 
CTCCTCATTC TGGACAGTTC GGCCAAACTG GGTGGATGTC AAACCATTCG CCTGTCGGAG 
CACATTACAC AGTTTGCCAC GCTGGAGGTG ATGCACTACT ATGGCATCCT CTTGGAGGAC 
TTCAACAACA ACGGAGAGTT TGTCAATGAC TGCATCTTCA CCATGATGCA TCACATAGGT 
GGCGATCTGG GCCAGATTGG GGTTCTATTC CAACCAATTA TTTTGAAAAC CTATTCAAGA 
ATTTGGGAAG CGGACTATGA ACTGTGCGAT GACTGGTCTG ATCTTATCGA GTATGTGATT 
CACAAGTTCA TGAATACTCC TCCGAAGTCG CCACTCACCA TTCCCACAAC TTCCTTAACG 
GAAATGACCA AGGAACACAA CCAGGAGCAT ACCGTTTGCT CATGGTCGCA GGAGGAAATG 
GATACACTGT ATTGGTATTA TGTGCAGAGC AAGAAGAACA ACGATATTGT GGGAAAGATA 
GTTAAGCTCT TCAGCAACAA CGGCAACAAG CTGAAAACCC GGATTTCTAT TATCCAACAA 
CTTTTGCAAC AAGACATTAT TACCCTGTTG GAGTACGATG ATCTGATGAA GTTCGAGGAT 
GCGGAGTATC AGAGAACTTT GCTGACCACT CCCACTTCCG CAACCACAGA GTCTGGAATT 
GAGATTAAGG AGTGCGCCTA CGGCAAACCC TCAGATGATG TGCAGATCCT GCTGGACCTG 
ATCATCAAGG AAAACAAAGC GCAGCATTTG TTATGGCTGC AGAGGATCCT CATCGAGTGC 
TGCTTCGTCA AGCTGACCCT GCGGAGTGGT CTCAAGGTTC CGGAAGGCGA TCACATCATG 
GAGCCGGTGG CCTATCACTG CATCTGCAAG CAGAAGTCCA TTCCGGTGGT GCAGTGGAAC 
AACGAGCAAT CCACTACGAT GCTGTACCAG CCCTTTGTGC TGCTGCTCCA CAAACTGGGC 
ATTCAGCTGC CGGCGGACGC GGGCTCGATC TTCGCCAGAA TTCCGGACTA CTGGACACCG 
GAGACCATGT ACGGACTGGC CAAGAAGCTG GGACCGCTGG ACAAACTCAA CCTAAAGTTC 
GACACCAGCG AACTGGAGGA TGCGACGGCG TCGAGTCCGT CGCGGTACCA CCACACCGGA 
CCCCGCAACT CGCTCAGCTC GGTGAGCAGC CTGGACGTGG ACCTCGGCGA GCCCGAGGAG 
CTGGCCCTCA TACCCGAGGT GGATGCGGCC GTGGAGAAGG CACACGCCAT GGCGTCCACG 
CCATCGCCCA GCGAGATGTT CGCGGTGCCC AAGACGAAGC ACTGCAACTC GATCATCAGA 
TACACACCCG ATCCCACGCC TCCAGTGCCC AACTGGCTGC AGTTGGTCAT GCGCAGCAAA 
TGCAATCAGC GCACAGGTCC GGCTGGTGAT CCCAGCGATT GCGTGGGCTC CTCGTCGACA 
ACAGTGGACG ATGAGGGATT GGGCAAGTCC ATCAGTGCAG CCACTTCGCA GGCGGCGAGC 
ACCTCCATGA GCACGGTTAA TCCCACAACC ACTTTGAGCC TCAATATGCT GAACACCTTC 
ATGGGAAGCC ACAACGAGAA CAGCAGCAGT TCCGGATGCG GAGGCACCGT GTCCTCCCTG 
TCCATGGTGG CTCTGATGAG CACTGGCGCG GTCGGCGGAG GAGGCAACAC CTCTGGACTG 
GACATGGATG TGGACGCCTC CATGAAGTCG TCGTTCGAGC GACTGGAGGT CAACGGATCG 
CACTTCTCGC GGGCCAACAA CTTGGACCAG GAGTACAGTG CTATGGTGGC ATCTGTGTAC 
GAAAAGGAGA AGGAATTAAA CAGCGACAAT GTCTCTTTGG CCTCGGACCT GACCAGAATG 
TATGTGAGCG ATGAGGACGA TCGACTTGAG CGAACCGAGA TCCGGGTGCC CCACTATCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Genome research23(1)89-98(2013 Jan)
Hu TT,Eisen MB,Thornton KR,Andolfatto P

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Dsim\tim cDNA ORF clone, Drosophila simulans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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