Dsim\GD15986 cDNA ORF clone, Drosophila simulans

Gene
Clones
gRNAs

Summary

Gene Symbol	Dsim\GD15986
Entrez Gene ID	6725685
Full Name	GD15986 gene product from transcript GD15986-RA
Synonyms	Dsim_GD15986,GD15986,dsim_GLEANR_16227
Gene Type	protein-coding
Organism	Drosophila simulans

mRNA and Protein(s)

mRNA	Protein	Name
XM_016172729.1	XP_016038945.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Hu TT, Eisen MB, Thornton KR, Andolfatto P
Genome research23(1)89-98(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dsim\GD15986 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dsim\GD15986 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODg16410	XM_016172729.1 Latest version!	Drosophila simulans uncharacterized protein (Dsim\GD15986), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dsim\GD15986 ( XM_016172729.1 ) cDNA ORF clone, Drosophila simulans -> XP_016038945.1 Drosophila simulans uncharacterized protein (Dsim\GD15986), mRNA.

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CloneID	ODg16410
Clone ID Related Accession (Same CDS sequence)	XM_016172729.1
Accession Version	XM_016172729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1459612800000
Organism	Drosophila simulans
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_029795). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACCC CCACGGACTC GAAGGCGCCG TTGCGCCAGG TGAAACGCGT ACAGTTCGGC 
ATTTTGTCGC CTGATGAAAT TCGTCGCATG TCCGTCACCG AGGGTGGCGT CCAGTTTGCA 
GAGACGATGG AGGGCGGACG ACCGAAGCTG GGCGGATTGA TGGATCCGCG CCAGGGTGTC 
ATAGACAGGA CGTCGCGCTG TCAGACGTGC GCCGGCAACA TGACCGAGTG CCCCGGTCAC 
TTTGGACACA TTGATCTGGC CAAACCAGTG TTCCACATCG GCTTCATCAC CAAGACAATT 
AAGATTTTGA GATGTGTGTG CTTCTACTGC TCCAAAATGC TTGTCTCGCC ACACAATCCA 
AAGATCAAGG AGATCGTGAT GAAGTCCAGG GGACAGCCGC GTAAGCGCCT GGCCTACGTC 
TATGATCTGT GCAAGGGCAA GACGATTTGC GAGGGCGGCG AGGACATGGA CCTCACCAAG 
GAGAACCAGC AGCCGGATCC AAACAAAAAG CCCGGCCACG GCGGTTGCGG TCACTACCAG 
CCGTCGATCC GGAGAACAGG ACTGGATCTC ACCGCCGAGT GGAAGCATCA GAACGAGGAT 
TCGCAGGAGA AGAAGATCGT GGTCTCGGCG GAGCGGGTTT GGGAAATCCT CAAGCATATC 
ACCGACGAGG AGTGCTTTAT TCTGGGCATG GATCCCAAGT ATGCGCGTCC CGATTGGATG 
ATCGTGACCG TCCTGCCTGT TCCACCGTTG GCCGTACGGC CCGCAGTCGT GATGTTCGGT 
GCTGCCAAGA ATCAGGATGA TTTGACACAC AAGCTGTCCG ATATCATCAA GGCAAACAAT 
GAGCTGCGCA AAAACGAGGC CAGTGGAGCG GCAGCGCATG TGATCCAGGA GAACATCAAG 
ATGCTGCAGT TCCACGTCGC CACACTGGTG GACAACGATA TGCCCGGCAT GCCCAGGGCT 
ATGCAAAAGT CGGGAAAACC CCTTAAAGCC ATCAAGGCGC GACTCAAGGG TAAGGAGGGT 
AGGATTCGTG GCAACTTGAT GGGCAAACGT GTGGACTTCT CCGCTCGTAC TGTCATCACA 
CCCGATCCAA ATCTGCGTAT CGATCAGGTC GGTGTGCCGC GTTCCATTGC CCAGAATCTA 
ACCTTCCCCG AGCTGGTCAC TCCCTTCAAC ATCGATCGCA TGCAGGAGCT GGTGCGCAGA 
GGTAACTCGC AGTATCCGGG TGCCAAGTAC ATTGTGCGCG ACAATGGCGA GCGTATTGAT 
CTTCGCTTCC ATCCTAAATC CTCTGACTTA CATCTGCAGT GCGGCTACAA AGTAGAGCGC 
CATTTGCGCG ACGACGATCT GGTGATCTTC AACCGACAGC CCACGCTCCA CAAGATGAGT 
ATGATGGGTC ACAGAGTGAA AGTGTTGCCC TGGTCGACGT TCCGCATGAA CCTGTCGTGT 
ACATCGCCCT ACAATGCTGA TTTCGACGGC GATGAAATGA ATCTGCACGT TCCGCAGTCC 
ATGGAGACGC GCGCCGAGGT GGAGAACATC CACATCACAC CCAGACAGAT CATCACGCCG 
CAGGCGAACA AACCTGTCAT GGGTATCGTG CAGGACACTC TGACTGCAGT GCGAAAGATG 
ACCAAGCGCG ACGTATTCAT CACGCGCGAG CAGGTGATGA ATCTGCTCAT GTTCCTGCCC 
ACGTGGGACG CTAAAATGCC GCAACCCTGC ATCCTGAAAC CGCGTCCCCT GTGGACGGGC 
AAACAGATCT TCTCCCTGAT CATCCCCGGC AACGTGAACA TGATACGCAC TCATTCCACG 
CATCCGGACG AGGAGGATGA AGGACCGTAC AAGTGGATCT CGCCCGGTGA CACCAAGGTA 
ATGGTGGAGC ACGGCGAACT TATCATGGGC ATTCTCTGCA AGAAGTCACT GGGTACATCA 
GCCGGATCAT TGCTGCATAT TTGTTTCCTG GAACTTGGTC ACGACATCGC TGGTCGGTTC 
TATGGCAACA TTCAGACCGT GATCAACAAT TGGTTGCTTT TCGAGGGCCA TAGTATCGGT 
ATTGGTGACA CCATTGCCGA TCCGCAGACC TACAACGAGA TACAGCAGGC CATCAAGAAG 
GCCAAGGACG ACGTGATAAA CGTTATCCAA AAGGCCCACA ACATGGAGCT GGAGCCCACG 
CCGGGTAACA CTCTGCGTCA GACGTTCGAG AACAAGGTGA ACCGTATCCT AAACGATGCT 
CGTGACAAGA CTGGTGGTTC GGCCAAGAAA TCTCTCACTG AGTACAACAA TCTAAAGGCT 
ATGGTGGTGT CTGGTTCCAA GGGTTCCAAC ATTAACATAT CCCAGGTTAT TGCTTGTGTG 
GGTCAACAGA ACGTGGAGGG TAAGCGAATC CCCTACGGTT TCCGCAAGCG CACTCTTCCC 
CACTTTATTA AGGACGATTA TGGTCCTGAG TCACGCGGTT TCGTGGAGAA TTCGTATCTT 
GCCGGCCTGA CACCCTCTGA GTTCTATTTC CACGCCATGG GTGGTCGTGA GGGTCTTATC 
GATACTGCTG TAAAGACAGC TGAAACCGGT TACATCCAGC GTCGTCTTAT AAAGGCTATG 
GAGTCGGTGA TGGTAAACTA CGACGGTACA GTGCGTAACT CGGTGGGCCA GCTTATTCAG 
CTGCGTTACG GCGAGGACGG TCTTTGCGGC GAGCTGGTTG AGTTCCAGAA CATGCCAACG 
GTGAAACTGT CGAACAAGTC GTTTGAAAAG CGCTTCAAAT TTGACTGGAG CAACGAGCGA 
TTAATGAAGA AGGTATTTAC GGATGATGTG ATCAAGGAGA TGACCGACAG CAGCGAAGCC 
ATCCAGGAAC TGGAGGCAGA GTGGGATCGT TTGGTTTCCG ATCGCGACAG TTTGAGACAA 
ATCTTCCCCA ACGGCGAATC CAAGGTGGTA CTGCCGTGCA ACCTGCAGCG TATGATCTGG 
AATGTGCAGA AGATCTTTCA CATCAACAAG CGCCTGCCCA CGGATCTCTC GCCCATTCGG 
GTGATCAAGG GCGTTAAGAC ATTGCTCGAA CGGTGTGTTA TTGTCACGGG AAATGATCGA 
ATTTCAAAGC AGGCCAACGA GAACGCCACG CTGCTATTCC AGTGCCTAAT CCGCTCGACA 
CTGTGTACGA AATACGTGTC GGAGGAGTTC CGCCTGTCCA CGGAGGCGTT TGAGTGGTTG 
GTCGGAGAAA TCGAGACGCG TTTCCAACAG GCCCAGGCCA ATCCCGGCGA GATGGTGGGT 
GCCCTGGCTG CCCAGAGTTT GGGTGAGCCT GCTACTCAGA TGACACTGAA CACCTTCCAT 
TTTGCTGGTG TGTCTTCAAA GAACGTAACA CTGGGTGTGC CTCGTCTCAA GGAGATCATC 
AACATATCCA AAAAGCCCAA AGCGCCGTCG CTAACCGTTT TCCTTACTGG CGGCGCTGCC 
CGCGATGCGG AGAAGGCCAA GAACGTACTG TGCCGCCTGG AGCATACCAC GCTGCGCAAG 
GTTACGGCCA ATACGGCTAT TTACTACGAC CCGGATCCGC AGAGAACGGT GATATCCGAG 
GATCAAGAGT TTGTGAATGT CTATTACGAA ATGCCCGACT TTGATCCCAC ACGCATTTCG 
CCCTGGTTGC TACGTATTGA ATTGGACCGC AAGCGGATGA CGGACAAGAA GCTGACAATG 
GAACAGATCG CCGAGAAGAT TAACGTGGGC TTCGGGGAGG ATCTTAATTG TATCTTCAAT 
GACGACAATG CCGATAAGCT GGTGCTGCGC ATTAGGATCA TGAACAACGA AGAGAACAAG 
TTCCAGGACG AAGACGAGGC CGTCGACAAG ATGGAGGACG ACATGTTCCT GCGCTGCATT 
GAGGCCAACA TGCTGTCGGA CATGACACTG CAGGGTATCG AAGCCATCGG CAAGGTGTAC 
ATGCATCTGC CACAGACCGA CAGCAAGAAG CGTATCGTGA TCACTGAGAC CGGAGAGTTT 
AAGGCCATCG GCGAGTGGCT GCTCGAGACG GACGGCACAT CGATGATGAA AGTGCTGTCT 
GAGCGTGACG TGGACCCAAT CCGAACATCC TCCAACGATA TCTGCGAGAT TTTCCAGGTG 
CTGGGCATCG AGGCGGTGCG AAAGTCTGTC GAAAAGGAGA TGAACGCCGT GCTGCAGTTC 
TACGGCCTGT ACGTGAACTA TCGTCACTTG GCCCTGTTGT GCGATGTGAT GACAGCCAAG 
GGCCATCTAA TGGCCATCAC CCGTCACGGC ATCAACAGGC AAGACACTGG TGCCCTTATG 
CGATGCTCCT TCGAGGAGAC AGTGGACGTG CTGATGGATG CCGCCGCTCA CGCCGAAACG 
GATCCCATGA GGGGCGTGTC TGAGAACATT ATCATGGGAC AGCTGCCCAA AATGGGTACT 
GGCTGCTTTG ACCTTCTGCT CGATGCAGAG AAGTGTCGTT TCGGCATCGA GATTCCCAAT 
ACGTTGGGCA ACAGTATGCT AGGTGGCGCT GCTATGTTCA TTGGCGGAGG ATCCACACCG 
AGCATGACGC CACCGATGAC GCCGTGGGTT AACTGCAACA CGCCGCGATA CTTCTCTCCA 
CCCGGCCACG TAAGTGCCAT GACTCCTGGC GGTCCCAGTT TCTCGCCTTC GGCTGCATCG 
GATGCGTCCG GAATGTCGCC TAGCTGGTCG CCGGCTCATC CGGGCTCATC GCCCAGTTCA 
CCAGGACCTT CGATGTCGCC GTACTTCCCA GCCTCGCCGA GTGTTTCTCC CTCTTATTCG 
CCAACAAGTC CGAACTACAC GGCATCTTCT CCCGGTGGAG CCTCGCCCAA TTACTCGCCC 
TCGAGTCCGA ACTATTCGCC GACATCGCCG CTTTATGCAA GTCCACGTTA CGCCTCGACA 
ACGCCAAATT TCAATCCCCA GTCGACGGGT TACTCGCCAT CTTCATCGGG ATACTCGCCA 
ACATCCCCGG GGTACTCGCC CACGGCACAG TTTCAGTCGA GTCCGTCGTT TGCGGGCAGC 
GGTAGCAACA TTTACTCGCC GGGCAATGCG TACTCGCCCA GCTCGTCCAA CTACTCACCC 
AATTCACCAT CCTACTCGCC GACATCACCA TCGTACTCGC CGTCGAGTCC TTCGTACTCA 
CCAACGTCGC CTTGCTATTC GCCCACATCG CCTTCGTACT CGCCAACGAG TCCGAACTAC 
ACACCCGTTA CACCCTCATA TTCGCCGACA AGTCCGAACT ACTCAGCGTC CCCGCAATAT 
TCTCCAGCCT CGCCAGCTTA CTCGCAAACG GGGGTGAAGT ACTCGCCTAC ATCGCCGACG 
TACTCGCCGC CGTCACCTTC GTACGATGGG TCTCCAGGAT CACCACAATA TACGCCAGGA 
TCTCCGCAGT ACTCGCCGGC CTCGCCTAAG TACTCGCCGA CCTCACCGCT GTACTCGCCT 
AGTTCGCCAC AGCACTCGCC CTCAAACCAG TACAGCCCAA CAGGATCGAC CTATTCGGCG 
ACGAGTCCGC GTTACTCGCC GAACATGTCC ATCTATTCGC CGAGCAGCAC CAAGTACTCG 
CCCACCTCGC CAACGTACAC ACCGACGGCC CGCAACTACT CGCCCACGTC ACCGATGTAC 
TCGCCAACGG CACCATCGCA CTACAGTCCC ACGAGTCCGG CCTACTCGCC CAGCAGTCCC 
ACGTTCGAGG AGAGCGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016038945.1
CDS	344..6007
Translation	MSTPTDSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTCAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRKRLAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEWKHQNEDSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVMFGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFSLIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHNMELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEKRFKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQCLIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDPDPQRTVISEDQEFVNVYYEMPDFDPTRISPWLLRIELDRKRMTDKKLTMEQIAEKINVGFGEDLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQGIEAIGKVYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIPNTLGNSMLGGAAMFIGGGSTPSMTPPMTPWVNCNTPRYFSPPGHVSAMTPGGPSFSPSAASDASGMSPSWSPAHPGSSPSSPGPSMSPYFPASPSVSPSYSPTSPNYTASSPGGASPNYSPSSPNYSPTSPLYASPRYASTTPNFNPQSTGYSPSSSGYSPTSPGYSPTAQFQSSPSFAGSGSNIYSPGNAYSPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSPTSPNYSASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPTSPLYSPSSPQHSPSNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTPTARNYSPTSPMYSPTAPSHYSPTSPAYSPSSPTFEESED

Target ORF information:

RefSeq Version	XM_016172729.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein (Dsim\GD15986), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016172729.1

ORF Insert Sequence:

ATGAGCACCC CCACGGACTC GAAGGCGCCG TTGCGCCAGG TGAAACGCGT ACAGTTCGGC 
ATTTTGTCGC CTGATGAAAT TCGTCGCATG TCCGTCACCG AGGGTGGCGT CCAGTTTGCA 
GAGACGATGG AGGGCGGACG ACCGAAGCTG GGCGGATTGA TGGATCCGCG CCAGGGTGTC 
ATAGACAGGA CGTCGCGCTG TCAGACGTGC GCCGGCAACA TGACCGAGTG CCCCGGTCAC 
TTTGGACACA TTGATCTGGC CAAACCAGTG TTCCACATCG GCTTCATCAC CAAGACAATT 
AAGATTTTGA GATGTGTGTG CTTCTACTGC TCCAAAATGC TTGTCTCGCC ACACAATCCA 
AAGATCAAGG AGATCGTGAT GAAGTCCAGG GGACAGCCGC GTAAGCGCCT GGCCTACGTC 
TATGATCTGT GCAAGGGCAA GACGATTTGC GAGGGCGGCG AGGACATGGA CCTCACCAAG 
GAGAACCAGC AGCCGGATCC AAACAAAAAG CCCGGCCACG GCGGTTGCGG TCACTACCAG 
CCGTCGATCC GGAGAACAGG ACTGGATCTC ACCGCCGAGT GGAAGCATCA GAACGAGGAT 
TCGCAGGAGA AGAAGATCGT GGTCTCGGCG GAGCGGGTTT GGGAAATCCT CAAGCATATC 
ACCGACGAGG AGTGCTTTAT TCTGGGCATG GATCCCAAGT ATGCGCGTCC CGATTGGATG 
ATCGTGACCG TCCTGCCTGT TCCACCGTTG GCCGTACGGC CCGCAGTCGT GATGTTCGGT 
GCTGCCAAGA ATCAGGATGA TTTGACACAC AAGCTGTCCG ATATCATCAA GGCAAACAAT 
GAGCTGCGCA AAAACGAGGC CAGTGGAGCG GCAGCGCATG TGATCCAGGA GAACATCAAG 
ATGCTGCAGT TCCACGTCGC CACACTGGTG GACAACGATA TGCCCGGCAT GCCCAGGGCT 
ATGCAAAAGT CGGGAAAACC CCTTAAAGCC ATCAAGGCGC GACTCAAGGG TAAGGAGGGT 
AGGATTCGTG GCAACTTGAT GGGCAAACGT GTGGACTTCT CCGCTCGTAC TGTCATCACA 
CCCGATCCAA ATCTGCGTAT CGATCAGGTC GGTGTGCCGC GTTCCATTGC CCAGAATCTA 
ACCTTCCCCG AGCTGGTCAC TCCCTTCAAC ATCGATCGCA TGCAGGAGCT GGTGCGCAGA 
GGTAACTCGC AGTATCCGGG TGCCAAGTAC ATTGTGCGCG ACAATGGCGA GCGTATTGAT 
CTTCGCTTCC ATCCTAAATC CTCTGACTTA CATCTGCAGT GCGGCTACAA AGTAGAGCGC 
CATTTGCGCG ACGACGATCT GGTGATCTTC AACCGACAGC CCACGCTCCA CAAGATGAGT 
ATGATGGGTC ACAGAGTGAA AGTGTTGCCC TGGTCGACGT TCCGCATGAA CCTGTCGTGT 
ACATCGCCCT ACAATGCTGA TTTCGACGGC GATGAAATGA ATCTGCACGT TCCGCAGTCC 
ATGGAGACGC GCGCCGAGGT GGAGAACATC CACATCACAC CCAGACAGAT CATCACGCCG 
CAGGCGAACA AACCTGTCAT GGGTATCGTG CAGGACACTC TGACTGCAGT GCGAAAGATG 
ACCAAGCGCG ACGTATTCAT CACGCGCGAG CAGGTGATGA ATCTGCTCAT GTTCCTGCCC 
ACGTGGGACG CTAAAATGCC GCAACCCTGC ATCCTGAAAC CGCGTCCCCT GTGGACGGGC 
AAACAGATCT TCTCCCTGAT CATCCCCGGC AACGTGAACA TGATACGCAC TCATTCCACG 
CATCCGGACG AGGAGGATGA AGGACCGTAC AAGTGGATCT CGCCCGGTGA CACCAAGGTA 
ATGGTGGAGC ACGGCGAACT TATCATGGGC ATTCTCTGCA AGAAGTCACT GGGTACATCA 
GCCGGATCAT TGCTGCATAT TTGTTTCCTG GAACTTGGTC ACGACATCGC TGGTCGGTTC 
TATGGCAACA TTCAGACCGT GATCAACAAT TGGTTGCTTT TCGAGGGCCA TAGTATCGGT 
ATTGGTGACA CCATTGCCGA TCCGCAGACC TACAACGAGA TACAGCAGGC CATCAAGAAG 
GCCAAGGACG ACGTGATAAA CGTTATCCAA AAGGCCCACA ACATGGAGCT GGAGCCCACG 
CCGGGTAACA CTCTGCGTCA GACGTTCGAG AACAAGGTGA ACCGTATCCT AAACGATGCT 
CGTGACAAGA CTGGTGGTTC GGCCAAGAAA TCTCTCACTG AGTACAACAA TCTAAAGGCT 
ATGGTGGTGT CTGGTTCCAA GGGTTCCAAC ATTAACATAT CCCAGGTTAT TGCTTGTGTG 
GGTCAACAGA ACGTGGAGGG TAAGCGAATC CCCTACGGTT TCCGCAAGCG CACTCTTCCC 
CACTTTATTA AGGACGATTA TGGTCCTGAG TCACGCGGTT TCGTGGAGAA TTCGTATCTT 
GCCGGCCTGA CACCCTCTGA GTTCTATTTC CACGCCATGG GTGGTCGTGA GGGTCTTATC 
GATACTGCTG TAAAGACAGC TGAAACCGGT TACATCCAGC GTCGTCTTAT AAAGGCTATG 
GAGTCGGTGA TGGTAAACTA CGACGGTACA GTGCGTAACT CGGTGGGCCA GCTTATTCAG 
CTGCGTTACG GCGAGGACGG TCTTTGCGGC GAGCTGGTTG AGTTCCAGAA CATGCCAACG 
GTGAAACTGT CGAACAAGTC GTTTGAAAAG CGCTTCAAAT TTGACTGGAG CAACGAGCGA 
TTAATGAAGA AGGTATTTAC GGATGATGTG ATCAAGGAGA TGACCGACAG CAGCGAAGCC 
ATCCAGGAAC TGGAGGCAGA GTGGGATCGT TTGGTTTCCG ATCGCGACAG TTTGAGACAA 
ATCTTCCCCA ACGGCGAATC CAAGGTGGTA CTGCCGTGCA ACCTGCAGCG TATGATCTGG 
AATGTGCAGA AGATCTTTCA CATCAACAAG CGCCTGCCCA CGGATCTCTC GCCCATTCGG 
GTGATCAAGG GCGTTAAGAC ATTGCTCGAA CGGTGTGTTA TTGTCACGGG AAATGATCGA 
ATTTCAAAGC AGGCCAACGA GAACGCCACG CTGCTATTCC AGTGCCTAAT CCGCTCGACA 
CTGTGTACGA AATACGTGTC GGAGGAGTTC CGCCTGTCCA CGGAGGCGTT TGAGTGGTTG 
GTCGGAGAAA TCGAGACGCG TTTCCAACAG GCCCAGGCCA ATCCCGGCGA GATGGTGGGT 
GCCCTGGCTG CCCAGAGTTT GGGTGAGCCT GCTACTCAGA TGACACTGAA CACCTTCCAT 
TTTGCTGGTG TGTCTTCAAA GAACGTAACA CTGGGTGTGC CTCGTCTCAA GGAGATCATC 
AACATATCCA AAAAGCCCAA AGCGCCGTCG CTAACCGTTT TCCTTACTGG CGGCGCTGCC 
CGCGATGCGG AGAAGGCCAA GAACGTACTG TGCCGCCTGG AGCATACCAC GCTGCGCAAG 
GTTACGGCCA ATACGGCTAT TTACTACGAC CCGGATCCGC AGAGAACGGT GATATCCGAG 
GATCAAGAGT TTGTGAATGT CTATTACGAA ATGCCCGACT TTGATCCCAC ACGCATTTCG 
CCCTGGTTGC TACGTATTGA ATTGGACCGC AAGCGGATGA CGGACAAGAA GCTGACAATG 
GAACAGATCG CCGAGAAGAT TAACGTGGGC TTCGGGGAGG ATCTTAATTG TATCTTCAAT 
GACGACAATG CCGATAAGCT GGTGCTGCGC ATTAGGATCA TGAACAACGA AGAGAACAAG 
TTCCAGGACG AAGACGAGGC CGTCGACAAG ATGGAGGACG ACATGTTCCT GCGCTGCATT 
GAGGCCAACA TGCTGTCGGA CATGACACTG CAGGGTATCG AAGCCATCGG CAAGGTGTAC 
ATGCATCTGC CACAGACCGA CAGCAAGAAG CGTATCGTGA TCACTGAGAC CGGAGAGTTT 
AAGGCCATCG GCGAGTGGCT GCTCGAGACG GACGGCACAT CGATGATGAA AGTGCTGTCT 
GAGCGTGACG TGGACCCAAT CCGAACATCC TCCAACGATA TCTGCGAGAT TTTCCAGGTG 
CTGGGCATCG AGGCGGTGCG AAAGTCTGTC GAAAAGGAGA TGAACGCCGT GCTGCAGTTC 
TACGGCCTGT ACGTGAACTA TCGTCACTTG GCCCTGTTGT GCGATGTGAT GACAGCCAAG 
GGCCATCTAA TGGCCATCAC CCGTCACGGC ATCAACAGGC AAGACACTGG TGCCCTTATG 
CGATGCTCCT TCGAGGAGAC AGTGGACGTG CTGATGGATG CCGCCGCTCA CGCCGAAACG 
GATCCCATGA GGGGCGTGTC TGAGAACATT ATCATGGGAC AGCTGCCCAA AATGGGTACT 
GGCTGCTTTG ACCTTCTGCT CGATGCAGAG AAGTGTCGTT TCGGCATCGA GATTCCCAAT 
ACGTTGGGCA ACAGTATGCT AGGTGGCGCT GCTATGTTCA TTGGCGGAGG ATCCACACCG 
AGCATGACGC CACCGATGAC GCCGTGGGTT AACTGCAACA CGCCGCGATA CTTCTCTCCA 
CCCGGCCACG TAAGTGCCAT GACTCCTGGC GGTCCCAGTT TCTCGCCTTC GGCTGCATCG 
GATGCGTCCG GAATGTCGCC TAGCTGGTCG CCGGCTCATC CGGGCTCATC GCCCAGTTCA 
CCAGGACCTT CGATGTCGCC GTACTTCCCA GCCTCGCCGA GTGTTTCTCC CTCTTATTCG 
CCAACAAGTC CGAACTACAC GGCATCTTCT CCCGGTGGAG CCTCGCCCAA TTACTCGCCC 
TCGAGTCCGA ACTATTCGCC GACATCGCCG CTTTATGCAA GTCCACGTTA CGCCTCGACA 
ACGCCAAATT TCAATCCCCA GTCGACGGGT TACTCGCCAT CTTCATCGGG ATACTCGCCA 
ACATCCCCGG GGTACTCGCC CACGGCACAG TTTCAGTCGA GTCCGTCGTT TGCGGGCAGC 
GGTAGCAACA TTTACTCGCC GGGCAATGCG TACTCGCCCA GCTCGTCCAA CTACTCACCC 
AATTCACCAT CCTACTCGCC GACATCACCA TCGTACTCGC CGTCGAGTCC TTCGTACTCA 
CCAACGTCGC CTTGCTATTC GCCCACATCG CCTTCGTACT CGCCAACGAG TCCGAACTAC 
ACACCCGTTA CACCCTCATA TTCGCCGACA AGTCCGAACT ACTCAGCGTC CCCGCAATAT 
TCTCCAGCCT CGCCAGCTTA CTCGCAAACG GGGGTGAAGT ACTCGCCTAC ATCGCCGACG 
TACTCGCCGC CGTCACCTTC GTACGATGGG TCTCCAGGAT CACCACAATA TACGCCAGGA 
TCTCCGCAGT ACTCGCCGGC CTCGCCTAAG TACTCGCCGA CCTCACCGCT GTACTCGCCT 
AGTTCGCCAC AGCACTCGCC CTCAAACCAG TACAGCCCAA CAGGATCGAC CTATTCGGCG 
ACGAGTCCGC GTTACTCGCC GAACATGTCC ATCTATTCGC CGAGCAGCAC CAAGTACTCG 
CCCACCTCGC CAACGTACAC ACCGACGGCC CGCAACTACT CGCCCACGTC ACCGATGTAC 
TCGCCAACGG CACCATCGCA CTACAGTCCC ACGAGTCCGG CCTACTCGCC CAGCAGTCCC 
ACGTTCGAGG AGAGCGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Genome research23(1)89-98(2013 Jan)
Hu TT,Eisen MB,Thornton KR,Andolfatto P

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Dsim\GD15986 cDNA ORF clone, Drosophila simulans

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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