Dpse\GA17997 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA17997
Entrez Gene ID	4816481
Full Name	GA17997 gene product from transcript GA17997-RA
Synonyms	GA17997,dpse_GLEANR_9106
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015181144.1	XP_015036630.1	uncharacterized protein, isoform B
XM_001355978.3	XP_001356014.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA17997 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA17997 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe10707	XM_001355978.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA17997), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe18570	XM_015181144.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17997), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA17997 ( XM_001355978.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001356014.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA17997), mRNA.

Price & Availability↑

CloneID	ODe10707
Clone ID Related Accession (Same CDS sequence)	XM_001355978.3
Accession Version	XM_001355978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). On Dec 22, 2015 this sequence version replaced XM_001355978.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAACT TTGACGCCTT CGAGCAGGGC CTTGCCAGCC CCAACGGAAA CGGAAGGCTG 
CCCAATGGAC ACGGCTGCCC GAAAGACAGC CCCAGCGTGG ACAACCAGCT GAAATTCTCG 
GCGCCCGCCT ACACTGAGAT TCGCACGAGG CGCTGCAACG CCAAGGCGGC CGCGGAGTCC 
AAGAATGCCA GCTCAAGCAC CTACCACCCG GAGAGGAAAG TGCTCTTGAA TGGACACGCC 
GCCGCGGCCA GGGAGCACAA GTCGGAGTGC AAGCCCAACC AGGCAGAGGG CAAGCTCTCG 
CCGGAGATGG AGCACTACCA GAAGACCTCG TTCGAAGAGG TGCCCATGCA CACGGCCTGC 
CTCACCTACC TGGGATTCTA CCTGCTCATG ATCCTGGGCT ACATCAACCA GCTGCTGTTC 
GTGCCCAAAG TGGCCACGGA GAAGGGCCGT GAGGGCTACG TGGCCCTGTA CGACGCCTTC 
GAGAGCTTCT ACTCCCGCTA CGTGTACCGC CGCATTAAGG ATTGCTGGAA CCGCCCCATC 
TGCAGTGTGC CCGGTGATGA GCTGACGCTC AAGGACCGCG TGACCGACGA CTATGGTTGG 
AGCTTCAAGT TTACGGGAAC GGAAACGCGT TGCCTCAATC TGGGCTCCTA CAACTATCTG 
GGCTTTGCCG CCGCCACAGG TCGCTGCGCC GACGAGTCCG AAGAGATGGC CCGCGACGCT 
GGCCTGGCTT ACTGCAGTTC CCGCTGTGAA CTGGGTAACA ATGCTCAGCT GGAGGAGCTG 
GAGAAGCTGA CTGCTCGCTA CTTTGGCGTG GAGGACGCTA TCGTTTTTGG CATGGGGTTC 
GCCACCAACG CCCTCAACCT GCCGTCTCTG CTCGGCCCGA ACTGCATGGT GGTGAGCGAT 
GAGAAGAACC ACGCCTCGAT CATCCTGGGC CTGCGTCTGT CCGGGGCCAC AATCAAGGTG 
TTCAAGCACA ACAACATGCG GGACTTGGAG CGCGTGCTGC GCGACGGCAT CTGCTACGGC 
AATACCAAGA ACGGAGGCAA ACCCTGGTCC AAGGTGATGA TCATTGTGGA GGGCATCTTC 
AGCATGGAGG GTTCCATAGT GCGCCTGCCC GAGGTCGTAG CCCTGAAGAA GAAGTACAAG 
GCATATCTGT ATCTAGACGA GGCCCACAGC GTGGGTGCTA TGGGCTCCCA TGGAAGGGGC 
GTCACGGACT ACTACAAGGT GGATCCAAAA GACGTCGACA TCCTAATGGG CACGTTCACC 
AAGAGCTTCG GCAGTGCTGG GGGCTATCTA GCCGGATCTA AGAAACTGAT TGACTTCCTT 
CGTACCAACA GCCATGCCCA CTGCTATGCT GGATCTATTT CGCCGCCTAT TGCTCAGCAG 
ATCCTCACCT CCATGAAGAC TATCATGGGC GAGGATGGCA CTGAGACGGG TCGCAACAAG 
ATCATGCAAC TGGCCAGGAA CACGCGGTAT TTCCGCCGTC GTCTGGCCCA GCTAGGAGTG 
ATAACCTACG GCCACGAAGA CTCGCCCGTG GTGCCCATGC TCGTCTACCT CTTCTCCAAG 
ATTGGTGCCG TGGTTCGCAC TTTGACCACC CGCCACATCG CCGTGGTGGG TGCCGGTTTT 
CCAGCCACAC CCATTATGGA GGGTCGCATC CGTTTCTGCC TGTCGGCTGC CCACACCAAA 
GAGCAGCTGG ACTATGCCCT TGAGGTTATT GACGAGACCA GCGACGATCT GGGCCTGAAG 
TATTCACGCA AGCCACGCGA TCCAAATCCG ATTGAATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001356014.1
CDS	256..2037
Translation	MGNFDAFEQGLASPNGNGRLPNGHGCPKDSPSVDNQLKFSAPAYTEIRTRRCNAKAAAESKNASSSTYHPERKVLLNGHAAAAREHKSECKPNQAEGKLSPEMEHYQKTSFEEVPMHTACLTYLGFYLLMILGYINQLLFVPKVATEKGREGYVALYDAFESFYSRYVYRRIKDCWNRPICSVPGDELTLKDRVTDDYGWSFKFTGTETRCLNLGSYNYLGFAAATGRCADESEEMARDAGLAYCSSRCELGNNAQLEELEKLTARYFGVEDAIVFGMGFATNALNLPSLLGPNCMVVSDEKNHASIILGLRLSGATIKVFKHNNMRDLERVLRDGICYGNTKNGGKPWSKVMIIVEGIFSMEGSIVRLPEVVALKKKYKAYLYLDEAHSVGAMGSHGRGVTDYYKVDPKDVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAGSISPPIAQQILTSMKTIMGEDGTETGRNKIMQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAVVGAGFPATPIMEGRIRFCLSAAHTKEQLDYALEVIDETSDDLGLKYSRKPRDPNPIEY

Target ORF information:

RefSeq Version	XM_001355978.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA17997), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355978.3

ORF Insert Sequence:

ATGGGAAACT TTGACGCCTT CGAGCAGGGC CTTGCCAGCC CCAACGGAAA CGGAAGGCTG 
CCCAATGGAC ACGGCTGCCC GAAAGACAGC CCCAGCGTGG ACAACCAGCT GAAATTCTCG 
GCGCCCGCCT ACACTGAGAT TCGCACGAGG CGCTGCAACG CCAAGGCGGC CGCGGAGTCC 
AAGAATGCCA GCTCAAGCAC CTACCACCCG GAGAGGAAAG TGCTCTTGAA TGGACACGCC 
GCCGCGGCCA GGGAGCACAA GTCGGAGTGC AAGCCCAACC AGGCAGAGGG CAAGCTCTCG 
CCGGAGATGG AGCACTACCA GAAGACCTCG TTCGAAGAGG TGCCCATGCA CACGGCCTGC 
CTCACCTACC TGGGATTCTA CCTGCTCATG ATCCTGGGCT ACATCAACCA GCTGCTGTTC 
GTGCCCAAAG TGGCCACGGA GAAGGGCCGT GAGGGCTACG TGGCCCTGTA CGACGCCTTC 
GAGAGCTTCT ACTCCCGCTA CGTGTACCGC CGCATTAAGG ATTGCTGGAA CCGCCCCATC 
TGCAGTGTGC CCGGTGATGA GCTGACGCTC AAGGACCGCG TGACCGACGA CTATGGTTGG 
AGCTTCAAGT TTACGGGAAC GGAAACGCGT TGCCTCAATC TGGGCTCCTA CAACTATCTG 
GGCTTTGCCG CCGCCACAGG TCGCTGCGCC GACGAGTCCG AAGAGATGGC CCGCGACGCT 
GGCCTGGCTT ACTGCAGTTC CCGCTGTGAA CTGGGTAACA ATGCTCAGCT GGAGGAGCTG 
GAGAAGCTGA CTGCTCGCTA CTTTGGCGTG GAGGACGCTA TCGTTTTTGG CATGGGGTTC 
GCCACCAACG CCCTCAACCT GCCGTCTCTG CTCGGCCCGA ACTGCATGGT GGTGAGCGAT 
GAGAAGAACC ACGCCTCGAT CATCCTGGGC CTGCGTCTGT CCGGGGCCAC AATCAAGGTG 
TTCAAGCACA ACAACATGCG GGACTTGGAG CGCGTGCTGC GCGACGGCAT CTGCTACGGC 
AATACCAAGA ACGGAGGCAA ACCCTGGTCC AAGGTGATGA TCATTGTGGA GGGCATCTTC 
AGCATGGAGG GTTCCATAGT GCGCCTGCCC GAGGTCGTAG CCCTGAAGAA GAAGTACAAG 
GCATATCTGT ATCTAGACGA GGCCCACAGC GTGGGTGCTA TGGGCTCCCA TGGAAGGGGC 
GTCACGGACT ACTACAAGGT GGATCCAAAA GACGTCGACA TCCTAATGGG CACGTTCACC 
AAGAGCTTCG GCAGTGCTGG GGGCTATCTA GCCGGATCTA AGAAACTGAT TGACTTCCTT 
CGTACCAACA GCCATGCCCA CTGCTATGCT GGATCTATTT CGCCGCCTAT TGCTCAGCAG 
ATCCTCACCT CCATGAAGAC TATCATGGGC GAGGATGGCA CTGAGACGGG TCGCAACAAG 
ATCATGCAAC TGGCCAGGAA CACGCGGTAT TTCCGCCGTC GTCTGGCCCA GCTAGGAGTG 
ATAACCTACG GCCACGAAGA CTCGCCCGTG GTGCCCATGC TCGTCTACCT CTTCTCCAAG 
ATTGGTGCCG TGGTTCGCAC TTTGACCACC CGCCACATCG CCGTGGTGGG TGCCGGTTTT 
CCAGCCACAC CCATTATGGA GGGTCGCATC CGTTTCTGCC TGTCGGCTGC CCACACCAAA 
GAGCAGCTGG ACTATGCCCT TGAGGTTATT GACGAGACCA GCGACGATCT GGGCCTGAAG 
TATTCACGCA AGCCACGCGA TCCAAATCCG ATTGAATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA17997 ( XM_015181144.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015036630.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17997), partial mRNA.

Price & Availability↑

CloneID	ODe18570
Clone ID Related Accession (Same CDS sequence)	XM_015181144.1
Accession Version	XM_015181144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589963). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGCATGCG TCATCTCGGA GAATGCCAGC TCAAGCACCT ACCACCCGGA GAGGAAAGTG 
CTCTTGAATG GACACGCCGC CGCGGCCAGG GAGCACAAGT CGGAGTGCAA GCCCAACCAG 
GCAGAGGGCA AGCTCTCGCC GGAGATGGAG CACTACCAGA AGACCTCGTT CGAAGAGGTG 
CCCATGCACA CGGCCTGCCT CACCTACCTG GGATTCTACC TGCTCATGAT CCTGGGCTAC 
ATCAACCAGC TGCTGTTCGT GCCCAAAGTG GCCACGGAGA AGGGCCGTGA GGGCTACGTG 
GCCCTGTACG ACGCCTTCGA GAGCTTCTAC TCCCGCTACG TGTACCGCCG CATTAAGGAT 
TGCTGGAACC GCCCCATCTG CAGTGTGCCC GGTGATGAGC TGACGCTCAA GGACCGCGTG 
ACCGACGACT ATGGTTGGAG CTTCAAGTTT ACGGGAACGG AAACGCGTTG CCTCAATCTG 
GGCTCCTACA ACTATCTGGG CTTTGCCGCC GCCACAGGTC GCTGCGCCGA CGAGTCCGAA 
GAGATGGCCC GCGACGCTGG CCTGGCTTAC TGCAGTTCCC GCTGTGAACT GGGTAACAAT 
GCTCAGCTGG AGGAGCTGGA GAAGCTGACT GCTCGCTACT TTGGCGTGGA GGACGCTATC 
GTTTTTGGCA TGGGGTTCGC CACCAACGCC CTCAACCTGC CGTCTCTGCT CGGCCCGAAC 
TGCATGGTGG TGAGCGATGA GAAGAACCAC GCCTCGATCA TCCTGGGCCT GCGTCTGTCC 
GGGGCCACAA TCAAGGTGTT CAAGCACAAC AACATGCGGG ACTTGGAGCG CGTGCTGCGC 
GACGGCATCT GCTACGGCAA TACCAAGAAC GGAGGCAAAC CCTGGTCCAA GGTGATGATC 
ATTGTGGAGG GCATCTTCAG CATGGAGGGT TCCATAGTGC GCCTGCCCGA GGTCGTAGCC 
CTGAAGAAGA AGTACAAGGC ATATCTGTAT CTAGACGAGG CCCACAGCGT GGGTGCTATG 
GGCTCCCATG GAAGGGGCGT CACGGACTAC TACAAGGTGG ATCCAAAAGA CGTCGACATC 
CTAATGGGCA CGTTCACCAA GAGCTTCGGC AGTGCTGGGG GCTATCTAGC CGGATCTAAG 
AAACTGATTG ACTTCCTTCG TACCAACAGC CATGCCCACT GCTATGCTGG ATCTATTTCG 
CCGCCTATTG CTCAGCAGAT CCTCACCTCC ATGAAGACTA TCATGGGCGA GGATGGCACT 
GAGACGGGTC GCAACAAGAT CATGCAACTG GCCAGGAACA CGCGGTATTT CCGCCGTCGT 
CTGGCCCAGC TAGGAGTGAT AACCTACGGC CACGAAGACT CGCCCGTGGT GCCCATGCTC 
GTCTACCTCT TCTCCAAGAT TGGTGCCGTG GTTCGCACTT TGACCACCCG CCACATCGCC 
GTGGTGGGTG CCGGTTTTCC AGCCACACCC ATTATGGAGG GTCGCATCCG TTTCTGCCTG 
TCGGCTGCCC ACACCAAAGA GCAGCTGGAC TATGCCCTTG AGGTTATTGA CGAGACCAGC 
GACGATCTGG GCCTGAAGTA TTCACGCAAG CCACGCGATC CAAATCCGAT TGAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015036630.1
CDS	1..1620
Translation	MACVISENASSSTYHPERKVLLNGHAAAAREHKSECKPNQAEGKLSPEMEHYQKTSFEEVPMHTACLTYLGFYLLMILGYINQLLFVPKVATEKGREGYVALYDAFESFYSRYVYRRIKDCWNRPICSVPGDELTLKDRVTDDYGWSFKFTGTETRCLNLGSYNYLGFAAATGRCADESEEMARDAGLAYCSSRCELGNNAQLEELEKLTARYFGVEDAIVFGMGFATNALNLPSLLGPNCMVVSDEKNHASIILGLRLSGATIKVFKHNNMRDLERVLRDGICYGNTKNGGKPWSKVMIIVEGIFSMEGSIVRLPEVVALKKKYKAYLYLDEAHSVGAMGSHGRGVTDYYKVDPKDVDILMGTFTKSFGSAGGYLAGSKKLIDFLRTNSHAHCYAGSISPPIAQQILTSMKTIMGEDGTETGRNKIMQLARNTRYFRRRLAQLGVITYGHEDSPVVPMLVYLFSKIGAVVRTLTTRHIAVVGAGFPATPIMEGRIRFCLSAAHTKEQLDYALEVIDETSDDLGLKYSRKPRDPNPIEY

Target ORF information:

RefSeq Version	XM_015181144.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17997), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015181144.1

ORF Insert Sequence:

ATGGCATGCG TCATCTCGGA GAATGCCAGC TCAAGCACCT ACCACCCGGA GAGGAAAGTG 
CTCTTGAATG GACACGCCGC CGCGGCCAGG GAGCACAAGT CGGAGTGCAA GCCCAACCAG 
GCAGAGGGCA AGCTCTCGCC GGAGATGGAG CACTACCAGA AGACCTCGTT CGAAGAGGTG 
CCCATGCACA CGGCCTGCCT CACCTACCTG GGATTCTACC TGCTCATGAT CCTGGGCTAC 
ATCAACCAGC TGCTGTTCGT GCCCAAAGTG GCCACGGAGA AGGGCCGTGA GGGCTACGTG 
GCCCTGTACG ACGCCTTCGA GAGCTTCTAC TCCCGCTACG TGTACCGCCG CATTAAGGAT 
TGCTGGAACC GCCCCATCTG CAGTGTGCCC GGTGATGAGC TGACGCTCAA GGACCGCGTG 
ACCGACGACT ATGGTTGGAG CTTCAAGTTT ACGGGAACGG AAACGCGTTG CCTCAATCTG 
GGCTCCTACA ACTATCTGGG CTTTGCCGCC GCCACAGGTC GCTGCGCCGA CGAGTCCGAA 
GAGATGGCCC GCGACGCTGG CCTGGCTTAC TGCAGTTCCC GCTGTGAACT GGGTAACAAT 
GCTCAGCTGG AGGAGCTGGA GAAGCTGACT GCTCGCTACT TTGGCGTGGA GGACGCTATC 
GTTTTTGGCA TGGGGTTCGC CACCAACGCC CTCAACCTGC CGTCTCTGCT CGGCCCGAAC 
TGCATGGTGG TGAGCGATGA GAAGAACCAC GCCTCGATCA TCCTGGGCCT GCGTCTGTCC 
GGGGCCACAA TCAAGGTGTT CAAGCACAAC AACATGCGGG ACTTGGAGCG CGTGCTGCGC 
GACGGCATCT GCTACGGCAA TACCAAGAAC GGAGGCAAAC CCTGGTCCAA GGTGATGATC 
ATTGTGGAGG GCATCTTCAG CATGGAGGGT TCCATAGTGC GCCTGCCCGA GGTCGTAGCC 
CTGAAGAAGA AGTACAAGGC ATATCTGTAT CTAGACGAGG CCCACAGCGT GGGTGCTATG 
GGCTCCCATG GAAGGGGCGT CACGGACTAC TACAAGGTGG ATCCAAAAGA CGTCGACATC 
CTAATGGGCA CGTTCACCAA GAGCTTCGGC AGTGCTGGGG GCTATCTAGC CGGATCTAAG 
AAACTGATTG ACTTCCTTCG TACCAACAGC CATGCCCACT GCTATGCTGG ATCTATTTCG 
CCGCCTATTG CTCAGCAGAT CCTCACCTCC ATGAAGACTA TCATGGGCGA GGATGGCACT 
GAGACGGGTC GCAACAAGAT CATGCAACTG GCCAGGAACA CGCGGTATTT CCGCCGTCGT 
CTGGCCCAGC TAGGAGTGAT AACCTACGGC CACGAAGACT CGCCCGTGGT GCCCATGCTC 
GTCTACCTCT TCTCCAAGAT TGGTGCCGTG GTTCGCACTT TGACCACCCG CCACATCGCC 
GTGGTGGGTG CCGGTTTTCC AGCCACACCC ATTATGGAGG GTCGCATCCG TTTCTGCCTG 
TCGGCTGCCC ACACCAAAGA GCAGCTGGAC TATGCCCTTG AGGTTATTGA CGAGACCAGC 
GACGATCTGG GCCTGAAGTA TTCACGCAAG CCACGCGATC CAAATCCGAT TGAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA17997 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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