Dpse\GA15365 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA15365
Entrez Gene ID	4805055
Full Name	GA15365 gene product from transcript GA15365-RA
Synonyms	GA15365,dpse_GLEANR_16059
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001361482.3	XP_001361519.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA15365 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA15365 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe04200	XM_001361482.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA15365), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA15365 ( XM_001361482.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001361519.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA15365), mRNA.

Price & Availability↑

CloneID	ODe04200
Clone ID Related Accession (Same CDS sequence)	XM_001361482.3
Accession Version	XM_001361482.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001361482.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGGG ACAGCCTACC CCGCGTACCG GACACCCACG GCGACGTGGT CGATGAGAAA 
TTATTCTCGG ATCTGTACAT ACGGACCAGC TGGGTGGATG CCCAAGTGGC GCTCGATCAA 
ATTGATAAGG GTAAAGCGCG TGGCAGCCGG ACGGCTATCT ATCTGCGTTC AGTATTCCAG 
TCCCACCTCG AAACGCTCGG CAGCTCTGTG CAGAAGCATG CGGGCAAGGT GCTATTCGTG 
GCCATCCTGG TGCTGAGCAC CTTCTGTGTG GGCCTGAAGA GCGCCCAGAT TCACTCGAAG 
GTGCACCAGC TCTGGATACA GGAGGGCGGC CGCCTCGAAT CGGAGCTGGC ATACACGCAG 
AAGACCATCG GCGAGGACGA GTCCTCCACG CATCAGCTGC TCATCCAGAC GGCCCACGAT 
CCGAATGCCT CGGTGCTGCA TCCGCAGGCG TTGCTCTCCC ACCTCGAGGT GCTGGTCAAG 
GCCACCGCCG TCAAGGTGCA CATGTACGAC GCCGAGTGGG GACTGCGAGA CATGTGCAAC 
TCCCCGACGA CGCCCACCTT CGAGGGGCAC TACTACATCG AGCAGATACT AAAGCATTTG 
ATACCGTGCT CGATCATCAC GCCGTTGGAT TGCTTCTGGG AGGGCAGCCA GCTGCTGGGA 
CCGGAGTTTC CCGTGAATAT ACCTGGCCTC AATCAGCGCC TCATGTGGAG CACTCTGAAC 
CCCGCGTCGG TGATGCAGTT CATGAAGCAG CAGATGTCCG ACCAGAAGAT CAGCTTCGAC 
TTCGAGACCG TCGAGCAGTA CCTGAAACGA GCGGCCATCG GCAGCGGCTA CATGGAGAAG 
CCCTGCCTCA ATCCGCTGAA TCCCAGTTGC CCGGAGACGG CCCCGAACAA GAACAGCACC 
ACGCCGCCAG ATGTGGGGGC GATACTGTCG GGCGGGTGCT ACGGCTATGC GGCCAAGCAC 
ATGCACTGGC CGGAGCAGCT GATTGTGGGC GGGGCGCAGC GGAATCGCAG CGGGCACCTG 
AAGAAGGCCC AGGCCCTGCA GACGGTGGTG CAGCTGATGA CCGAGAAGGA GATGTACGAC 
CAGTGGGAGG ACAATTACAA GGTGCACCAC ATCGGCTGGA CCCAGGAGAA GGCCGCCGAG 
GTGCTGAATG CCTGGCAGCG GAACTTCTCC CGCGAGGTGG AGCTCCTCCT GCGCAAACAG 
TCCCGCATTG CGGCCAACTA CGACATCTAC GTGTTCAGCT CGGCCGCCCT GGACGACATC 
CTGGCCAAGT TCTCGCATCC GAGTGCCCTG AGCATCGTTA TCGGCGTCGC CGTCACCGTC 
CTTTACGCCT TTTGCACGCT CCTCCGCTGG CGGGATCCTG TTCGGGGCCA GAGCAGCGTG 
GGTGTTGCTG GTGTCCTGCT CATGTGCTTT AGCACGGCGG CTGGCCTGGG ACTGTGTGCC 
CTGATGGGGA TCGTCTTCAA TGCGGCCAGC ACCCAGGTGG TGCCGTTCCT GGCCCTGGGT 
CTCGGAGTGG ACCACATCTT TATGCTGACG GCGGCGTATG CGGAAAGCAA TCGCAAGGAG 
CAGACCAAGC TGATCCTGAA GAAGGTCGGT CCCAGCATCC TGTTCAGCGC CTGCAGCACG 
GCCGGATCCT TCTTCGCGGC CGCCTTCATT CCAGTGCCTG CGCTGAAGGT CTTCTGCCTG 
CAGGCGGCCA TCGTGATGTG CTTCAATCTG GCGGCGGCTC TGCTGGTCTT CCCGGCCATG 
ATCTCCTTGG ATCTGCGGCG GCGCACAGCC GGACGGGCGG ACATTTTCTG CTGCTGCTTT 
CCCGTGTGGA AGGAGCAGTC CAAAGTGATG CCGATGAACA ACAACAACAG CCTGCGGGGA 
GCACGGCACG TGAAGAACTG CAACAACAAC CGCGTGGGTC TGCCTCCCCA GCCACCATCC 
CAGAACCCCC TTCTGGAGCA GCACGCCAGC GGCGTCCCCT CTGCCAGCAG CAAAAGCAGC 
AGCAGCAACT CCCTGCCGTC CTTCTCGCTG TCCAGCTTCG CCTTCGAGTA CTACACGCCC 
TTCCTGATGC GCAGCTGGGT GAAATTTCTG GCCGTGATGG GCTTCCTGGT GGCGCTGATC 
TTCAGTCTGT ACGCCTCCAC CAAGCTGCAG GATGGCCTGG ACATCATCGA CCTGGTGCCA 
AAGCACAGCA ACGAGCACAA GTTCCTCGAC GCGCAGACGC GCCTCTTCGG CTTTTACAGC 
ATGTACGCGG TGACGCAAGG CAATTTCGAG TACCCCACGC AGCAGCAGCT CCTGAGGGAC 
TACCACGACG CATTCGTCCG AGTCCCGCAT GTGATCAAGA ACGATAACGG GGGCCTGCCG 
GACTTCTGGC TGCTGCTGTT CCGCGACTGG CTTAGCAATC TGCAGCGCAT CTTCGACGAT 
GAGATCCGGG ACGGGCGACT GAACAAGGAG GTGTGGTACC CGAATGCCAG CAGCGATGCC 
ATCCTGGCGT ACAAGCTGAT CGTGCAGACG GGCCATGTGG ACAATCCGGT GGACAAGGAG 
CTGGTGAAGA CGAATCGGCT GGTCTTCAGC GATGGCATCA TCAATCCGAA GGCCTTCTAC 
AACTATCTCT CTGCCTGGGC CACCAACGAT GTCTTCGCCT ATGGAGCCTC TCAGGGCAAA 
CTCTATCCGG AACCGCGTCA GTATTATCAT GCGCCCAACG AGTACGACCT GAAGATACCC 
AAGAGCCTGC CCCTGGTCTA CGCACAGATG CCCTTCTATC TGCACGGCCT GACGGACACC 
TCGCAGATCA AGACCCTGAT CGGACACATA CGCGACCTGA GCGTTAAGTT CGAGGGCTTT 
GGTCTGCCCA ACTATCCCTC CGGCATTCCA TTCATCTTCT GGGAGCAGTA CATGACGTTA 
CGCTCCTCGC TGGCCATGAT CCTGGCGTGT GTCTTGCTGG CTGCCTTGGT GCTGGTCTCC 
CTGCTGCTGC TGTCCGTTTG GGCCGCCGTG CTGGTGATCC TCAGCGTCTT GGCCTCGCTG 
GCCCAGATCT TTGGGGCCAT GACCTTGCTG GGCATCAAGC TGTCGGCCAT ACCCGCTGTC 
ATACTCATAC TGAGCGTGGG CATGATGCTC TGCTTCAATG TGCACATCTC CCTGGGCTTT 
ATGACTTCGT TGGGCAATCG TCAACGTCGC GTCCATCTGG CCATGCAGCT GTCATTGGGT 
CCCCTGGTGC ATGGGATGCT CACCTCTGGG GTGGCCGTTT TCATGCTCTC CACGTCCCCC 
TTCGAGTTCG TCATCCGCCA CTTCTGCTTC CTGCTGATCG TGGTCCTCTG CGTGGGGGCC 
TGCAACAGTC TGGTGGTCTT CCCCATTCTG CTGAGCATGC TGGGGCCAGA GGCGGAGCTT 
GTGCCCTTGG AGCATCCCGA TAGGATATCC ACGCCCTCGC CGCTGCCCGT GCGCAGCAGC 
AAGCGTTCGA ACAAGTCGTT CAGTGGGAAC GGCTCGCGTT CGTCGTCGCG CAACAACTGC 
CAGAAGGCGC ACCACTACCA CAAGGACGTC AACATCAACG ATCCCTCGCT GACGACCATT 
ACGGAGGAGC CGCAGTCTTG GAAGTCCAGC AACTCCTCCA TCCAGATGCA CAACGACTGG 
AGTGCCCCCG CACCGCCGCC GGCACAGACA ACGAATAACT TCAACAACAG CAACTATCAG 
CATGGCGGGG GATCGCGACC CGCCTCGTAT GGAGCACCAC CACCCGCTTA CCACAAGGCC 
GCACAGCAGC AGCAGCAGCA GTATCCGCCG GAACTGCAGA GCATCGTTGT CCAGCCGGAG 
GTGACGGTGG AGACGACGCA CTCGGACAGC AACACCACCA AGGTGACGGC CACGGCCAAC 
ATCAAGGTGG AGCTGGTCAC GCCCGGGCGG GCGGTGCGCA GCTATAACTT TACGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001361519.1
CDS	817..4716
Translation	MDRDSLPRVPDTHGDVVDEKLFSDLYIRTSWVDAQVALDQIDKGKARGSRTAIYLRSVFQSHLETLGSSVQKHAGKVLFVAILVLSTFCVGLKSAQIHSKVHQLWIQEGGRLESELAYTQKTIGEDESSTHQLLIQTAHDPNASVLHPQALLSHLEVLVKATAVKVHMYDAEWGLRDMCNSPTTPTFEGHYYIEQILKHLIPCSIITPLDCFWEGSQLLGPEFPVNIPGLNQRLMWSTLNPASVMQFMKQQMSDQKISFDFETVEQYLKRAAIGSGYMEKPCLNPLNPSCPETAPNKNSTTPPDVGAILSGGCYGYAAKHMHWPEQLIVGGAQRNRSGHLKKAQALQTVVQLMTEKEMYDQWEDNYKVHHIGWTQEKAAEVLNAWQRNFSREVELLLRKQSRIAANYDIYVFSSAALDDILAKFSHPSALSIVIGVAVTVLYAFCTLLRWRDPVRGQSSVGVAGVLLMCFSTAAGLGLCALMGIVFNAASTQVVPFLALGLGVDHIFMLTAAYAESNRKEQTKLILKKVGPSILFSACSTAGSFFAAAFIPVPALKVFCLQAAIVMCFNLAAALLVFPAMISLDLRRRTAGRADIFCCCFPVWKEQSKVMPMNNNNSLRGARHVKNCNNNRVGLPPQPPSQNPLLEQHASGVPSASSKSSSSNSLPSFSLSSFAFEYYTPFLMRSWVKFLAVMGFLVALIFSLYASTKLQDGLDIIDLVPKHSNEHKFLDAQTRLFGFYSMYAVTQGNFEYPTQQQLLRDYHDAFVRVPHVIKNDNGGLPDFWLLLFRDWLSNLQRIFDDEIRDGRLNKEVWYPNASSDAILAYKLIVQTGHVDNPVDKELVKTNRLVFSDGIINPKAFYNYLSAWATNDVFAYGASQGKLYPEPRQYYHAPNEYDLKIPKSLPLVYAQMPFYLHGLTDTSQIKTLIGHIRDLSVKFEGFGLPNYPSGIPFIFWEQYMTLRSSLAMILACVLLAALVLVSLLLLSVWAAVLVILSVLASLAQIFGAMTLLGIKLSAIPAVILILSVGMMLCFNVHISLGFMTSLGNRQRRVHLAMQLSLGPLVHGMLTSGVAVFMLSTSPFEFVIRHFCFLLIVVLCVGACNSLVVFPILLSMLGPEAELVPLEHPDRISTPSPLPVRSSKRSNKSFSGNGSRSSSRNNCQKAHHYHKDVNINDPSLTTITEEPQSWKSSNSSIQMHNDWSAPAPPPAQTTNNFNNSNYQHGGGSRPASYGAPPPAYHKAAQQQQQQYPPELQSIVVQPEVTVETTHSDSNTTKVTATANIKVELVTPGRAVRSYNFTS

Target ORF information:

RefSeq Version	XM_001361482.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA15365), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001361482.3

ORF Insert Sequence:

ATGGACAGGG ACAGCCTACC CCGCGTACCG GACACCCACG GCGACGTGGT CGATGAGAAA 
TTATTCTCGG ATCTGTACAT ACGGACCAGC TGGGTGGATG CCCAAGTGGC GCTCGATCAA 
ATTGATAAGG GTAAAGCGCG TGGCAGCCGG ACGGCTATCT ATCTGCGTTC AGTATTCCAG 
TCCCACCTCG AAACGCTCGG CAGCTCTGTG CAGAAGCATG CGGGCAAGGT GCTATTCGTG 
GCCATCCTGG TGCTGAGCAC CTTCTGTGTG GGCCTGAAGA GCGCCCAGAT TCACTCGAAG 
GTGCACCAGC TCTGGATACA GGAGGGCGGC CGCCTCGAAT CGGAGCTGGC ATACACGCAG 
AAGACCATCG GCGAGGACGA GTCCTCCACG CATCAGCTGC TCATCCAGAC GGCCCACGAT 
CCGAATGCCT CGGTGCTGCA TCCGCAGGCG TTGCTCTCCC ACCTCGAGGT GCTGGTCAAG 
GCCACCGCCG TCAAGGTGCA CATGTACGAC GCCGAGTGGG GACTGCGAGA CATGTGCAAC 
TCCCCGACGA CGCCCACCTT CGAGGGGCAC TACTACATCG AGCAGATACT AAAGCATTTG 
ATACCGTGCT CGATCATCAC GCCGTTGGAT TGCTTCTGGG AGGGCAGCCA GCTGCTGGGA 
CCGGAGTTTC CCGTGAATAT ACCTGGCCTC AATCAGCGCC TCATGTGGAG CACTCTGAAC 
CCCGCGTCGG TGATGCAGTT CATGAAGCAG CAGATGTCCG ACCAGAAGAT CAGCTTCGAC 
TTCGAGACCG TCGAGCAGTA CCTGAAACGA GCGGCCATCG GCAGCGGCTA CATGGAGAAG 
CCCTGCCTCA ATCCGCTGAA TCCCAGTTGC CCGGAGACGG CCCCGAACAA GAACAGCACC 
ACGCCGCCAG ATGTGGGGGC GATACTGTCG GGCGGGTGCT ACGGCTATGC GGCCAAGCAC 
ATGCACTGGC CGGAGCAGCT GATTGTGGGC GGGGCGCAGC GGAATCGCAG CGGGCACCTG 
AAGAAGGCCC AGGCCCTGCA GACGGTGGTG CAGCTGATGA CCGAGAAGGA GATGTACGAC 
CAGTGGGAGG ACAATTACAA GGTGCACCAC ATCGGCTGGA CCCAGGAGAA GGCCGCCGAG 
GTGCTGAATG CCTGGCAGCG GAACTTCTCC CGCGAGGTGG AGCTCCTCCT GCGCAAACAG 
TCCCGCATTG CGGCCAACTA CGACATCTAC GTGTTCAGCT CGGCCGCCCT GGACGACATC 
CTGGCCAAGT TCTCGCATCC GAGTGCCCTG AGCATCGTTA TCGGCGTCGC CGTCACCGTC 
CTTTACGCCT TTTGCACGCT CCTCCGCTGG CGGGATCCTG TTCGGGGCCA GAGCAGCGTG 
GGTGTTGCTG GTGTCCTGCT CATGTGCTTT AGCACGGCGG CTGGCCTGGG ACTGTGTGCC 
CTGATGGGGA TCGTCTTCAA TGCGGCCAGC ACCCAGGTGG TGCCGTTCCT GGCCCTGGGT 
CTCGGAGTGG ACCACATCTT TATGCTGACG GCGGCGTATG CGGAAAGCAA TCGCAAGGAG 
CAGACCAAGC TGATCCTGAA GAAGGTCGGT CCCAGCATCC TGTTCAGCGC CTGCAGCACG 
GCCGGATCCT TCTTCGCGGC CGCCTTCATT CCAGTGCCTG CGCTGAAGGT CTTCTGCCTG 
CAGGCGGCCA TCGTGATGTG CTTCAATCTG GCGGCGGCTC TGCTGGTCTT CCCGGCCATG 
ATCTCCTTGG ATCTGCGGCG GCGCACAGCC GGACGGGCGG ACATTTTCTG CTGCTGCTTT 
CCCGTGTGGA AGGAGCAGTC CAAAGTGATG CCGATGAACA ACAACAACAG CCTGCGGGGA 
GCACGGCACG TGAAGAACTG CAACAACAAC CGCGTGGGTC TGCCTCCCCA GCCACCATCC 
CAGAACCCCC TTCTGGAGCA GCACGCCAGC GGCGTCCCCT CTGCCAGCAG CAAAAGCAGC 
AGCAGCAACT CCCTGCCGTC CTTCTCGCTG TCCAGCTTCG CCTTCGAGTA CTACACGCCC 
TTCCTGATGC GCAGCTGGGT GAAATTTCTG GCCGTGATGG GCTTCCTGGT GGCGCTGATC 
TTCAGTCTGT ACGCCTCCAC CAAGCTGCAG GATGGCCTGG ACATCATCGA CCTGGTGCCA 
AAGCACAGCA ACGAGCACAA GTTCCTCGAC GCGCAGACGC GCCTCTTCGG CTTTTACAGC 
ATGTACGCGG TGACGCAAGG CAATTTCGAG TACCCCACGC AGCAGCAGCT CCTGAGGGAC 
TACCACGACG CATTCGTCCG AGTCCCGCAT GTGATCAAGA ACGATAACGG GGGCCTGCCG 
GACTTCTGGC TGCTGCTGTT CCGCGACTGG CTTAGCAATC TGCAGCGCAT CTTCGACGAT 
GAGATCCGGG ACGGGCGACT GAACAAGGAG GTGTGGTACC CGAATGCCAG CAGCGATGCC 
ATCCTGGCGT ACAAGCTGAT CGTGCAGACG GGCCATGTGG ACAATCCGGT GGACAAGGAG 
CTGGTGAAGA CGAATCGGCT GGTCTTCAGC GATGGCATCA TCAATCCGAA GGCCTTCTAC 
AACTATCTCT CTGCCTGGGC CACCAACGAT GTCTTCGCCT ATGGAGCCTC TCAGGGCAAA 
CTCTATCCGG AACCGCGTCA GTATTATCAT GCGCCCAACG AGTACGACCT GAAGATACCC 
AAGAGCCTGC CCCTGGTCTA CGCACAGATG CCCTTCTATC TGCACGGCCT GACGGACACC 
TCGCAGATCA AGACCCTGAT CGGACACATA CGCGACCTGA GCGTTAAGTT CGAGGGCTTT 
GGTCTGCCCA ACTATCCCTC CGGCATTCCA TTCATCTTCT GGGAGCAGTA CATGACGTTA 
CGCTCCTCGC TGGCCATGAT CCTGGCGTGT GTCTTGCTGG CTGCCTTGGT GCTGGTCTCC 
CTGCTGCTGC TGTCCGTTTG GGCCGCCGTG CTGGTGATCC TCAGCGTCTT GGCCTCGCTG 
GCCCAGATCT TTGGGGCCAT GACCTTGCTG GGCATCAAGC TGTCGGCCAT ACCCGCTGTC 
ATACTCATAC TGAGCGTGGG CATGATGCTC TGCTTCAATG TGCACATCTC CCTGGGCTTT 
ATGACTTCGT TGGGCAATCG TCAACGTCGC GTCCATCTGG CCATGCAGCT GTCATTGGGT 
CCCCTGGTGC ATGGGATGCT CACCTCTGGG GTGGCCGTTT TCATGCTCTC CACGTCCCCC 
TTCGAGTTCG TCATCCGCCA CTTCTGCTTC CTGCTGATCG TGGTCCTCTG CGTGGGGGCC 
TGCAACAGTC TGGTGGTCTT CCCCATTCTG CTGAGCATGC TGGGGCCAGA GGCGGAGCTT 
GTGCCCTTGG AGCATCCCGA TAGGATATCC ACGCCCTCGC CGCTGCCCGT GCGCAGCAGC 
AAGCGTTCGA ACAAGTCGTT CAGTGGGAAC GGCTCGCGTT CGTCGTCGCG CAACAACTGC 
CAGAAGGCGC ACCACTACCA CAAGGACGTC AACATCAACG ATCCCTCGCT GACGACCATT 
ACGGAGGAGC CGCAGTCTTG GAAGTCCAGC AACTCCTCCA TCCAGATGCA CAACGACTGG 
AGTGCCCCCG CACCGCCGCC GGCACAGACA ACGAATAACT TCAACAACAG CAACTATCAG 
CATGGCGGGG GATCGCGACC CGCCTCGTAT GGAGCACCAC CACCCGCTTA CCACAAGGCC 
GCACAGCAGC AGCAGCAGCA GTATCCGCCG GAACTGCAGA GCATCGTTGT CCAGCCGGAG 
GTGACGGTGG AGACGACGCA CTCGGACAGC AACACCACCA AGGTGACGGC CACGGCCAAC 
ATCAAGGTGG AGCTGGTCAC GCCCGGGCGG GCGGTGCGCA GCTATAACTT TACGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA15365 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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