Dpse\GA20656 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20656
Entrez Gene ID	4804849
Full Name	GA20656 gene product from transcript GA20656-RA
Synonyms	GA20656,dpse_GLEANR_15918
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015184750.1	XP_015040236.1	uncharacterized protein, isoform B
XM_001361306.3	XP_001361343.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20656 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20656 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe03991	XM_001361306.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20656), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe23197	XM_015184750.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20656), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20656 ( XM_001361306.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001361343.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20656), mRNA.

Price & Availability↑

CloneID	ODe03991
Clone ID Related Accession (Same CDS sequence)	XM_001361306.3
Accession Version	XM_001361306.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001361306.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC CAAAAGCCAG CGAACCCGTG GAGGGGCAAT CATTAAGTCA GGCAGAGGCA 
CCGCTAATGG CACACGAAAA GAAACAGACC CAGCAGGATC AGCCCAAATG GAGCTTTTCC 
CTCAATTTGG CTTGCGTTGG ATCCTCGATT GGAGCCGCCT TGCCCGTGGG CTACTGCACT 
GGGGTCATGA ACAGCCCCGC CGTGCTTATG CGCTCCTGGT GCAACGAAAC TCTGATTGCG 
CGATATGATC TTCACCTGGG AGCCTCGGGC CTGGAGTGGC TCTGGTCGGC CGTCGTCTCC 
ATCTTTCTGG TGGGTGGAGC CATCGGTTCT ATGACGGGCG CATCTACCGC CAACCGCTTT 
GGACGCCGCG GCTGCTTCTA CATATGCGGT ATGCTACTCG CCCTTGGTGC CGTCTGCTTC 
GCTGTATGCC GCTTGTTGGT ATCCGTGGAG CTGCTGCTCC TGGGCCGATT GCTCGTGGGG 
CTGGCGGGGG GCCTCGTCAC CGCCTTCATG CCCATGTACC ACAGCGAACT GGCCGCCCTC 
TCCCAGCGAA GTACTCTGTC TCCGTGTTGC GCAATGGGCC TGACCGCAGG CGTGGTGGTA 
GCCCAGATCT GCAGCCTACA GTCGCTTTTC GGGGGGGCAG ATCGGTGGCA CATTGCTTTG 
TCCTTTTTTG GGCTGCTGGT ACTGCTCTGC TATGCCCCAT TCCGGTGGTA CCCCGAGAGC 
CCCAAGTGGC TGTACATAGT CAAGGGCCGC AAGGAGGAGG CCAGACAACA GCTCCAGCTG 
TTGCGGGGCT ATTCCTCGGA CAGCGCGGCT CTGCAGGCGG AGCTAAACGA AATGGAGCTG 
GAGGCATCGG CCAAGGTGGA GGCTAGTGGA TTCATGGAAG TGCTGCGCAA TCCCCGTCTG 
CGCCTGCCAC TCATCATTGT TTGCGCCTTT CTTGGTGGCC AGCAGCTGTC GGGTATCAAT 
GCGATATTCT TCTACTCTGT CTCCATATTC CGCAAGGCAG GGCTGTCCAC GCAGGCGGCG 
GAGTGGGCCA ATCTGGGTGC TGGATCCCTC AATCTGGCCA CATCGATGGT GGGTCCGGTC 
CTGTTGGAGC GCGTGAATCG TCGCCCGCTG ATGCTCTTCT CCACCTTCTT CTGCGCCGTA 
TGCCTTTTTC TCTTCGCCAT GATGCTCTAC TTCATTGAGA GCTTTAGCTG GTTTGCCATG 
GGCTGCATTG GCTGCATCTT CCTGTACATA TTCTTCTATC AGTTCGGTGT CGGGCCCATG 
CCGTTCTTCA TAGGAGCCGA ACTGTTTGAG GTGTCCCCGC GTCCGGCTGC CATGGCTTTG 
GGTAGCGTTG TCTACTGGGT ATGCAATTTC ATCATTGGCA TGGCCTTTCC CACGCTGCAG 
AATGCCTGGG GCGCCCTGGT CTTCCTCCCA TTCTCCATCG CCTGCTTGCT GCTCTTTGGG 
CTGACCAAAC GCTACTTGCC GGAGACTCGC GGCCGCGATC CATCCGAGGT TGCTCCGCTC 
GTCGCCGACG GCTTTAAATC AAAAGTCTTG CGCACTCGCA CTGCGAGCCA ATCTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001361343.2
CDS	84..1643
Translation	MEKPKASEPVEGQSLSQAEAPLMAHEKKQTQQDQPKWSFSLNLACVGSSIGAALPVGYCTGVMNSPAVLMRSWCNETLIARYDLHLGASGLEWLWSAVVSIFLVGGAIGSMTGASTANRFGRRGCFYICGMLLALGAVCFAVCRLLVSVELLLLGRLLVGLAGGLVTAFMPMYHSELAALSQRSTLSPCCAMGLTAGVVVAQICSLQSLFGGADRWHIALSFFGLLVLLCYAPFRWYPESPKWLYIVKGRKEEARQQLQLLRGYSSDSAALQAELNEMELEASAKVEASGFMEVLRNPRLRLPLIIVCAFLGGQQLSGINAIFFYSVSIFRKAGLSTQAAEWANLGAGSLNLATSMVGPVLLERVNRRPLMLFSTFFCAVCLFLFAMMLYFIESFSWFAMGCIGCIFLYIFFYQFGVGPMPFFIGAELFEVSPRPAAMALGSVVYWVCNFIIGMAFPTLQNAWGALVFLPFSIACLLLFGLTKRYLPETRGRDPSEVAPLVADGFKSKVLRTRTASQSS

Target ORF information:

RefSeq Version	XM_001361306.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20656), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001361306.3

ORF Insert Sequence:

ATGGAAAAGC CAAAAGCCAG CGAACCCGTG GAGGGGCAAT CATTAAGTCA GGCAGAGGCA 
CCGCTAATGG CACACGAAAA GAAACAGACC CAGCAGGATC AGCCCAAATG GAGCTTTTCC 
CTCAATTTGG CTTGCGTTGG ATCCTCGATT GGAGCCGCCT TGCCCGTGGG CTACTGCACT 
GGGGTCATGA ACAGCCCCGC CGTGCTTATG CGCTCCTGGT GCAACGAAAC TCTGATTGCG 
CGATATGATC TTCACCTGGG AGCCTCGGGC CTGGAGTGGC TCTGGTCGGC CGTCGTCTCC 
ATCTTTCTGG TGGGTGGAGC CATCGGTTCT ATGACGGGCG CATCTACCGC CAACCGCTTT 
GGACGCCGCG GCTGCTTCTA CATATGCGGT ATGCTACTCG CCCTTGGTGC CGTCTGCTTC 
GCTGTATGCC GCTTGTTGGT ATCCGTGGAG CTGCTGCTCC TGGGCCGATT GCTCGTGGGG 
CTGGCGGGGG GCCTCGTCAC CGCCTTCATG CCCATGTACC ACAGCGAACT GGCCGCCCTC 
TCCCAGCGAA GTACTCTGTC TCCGTGTTGC GCAATGGGCC TGACCGCAGG CGTGGTGGTA 
GCCCAGATCT GCAGCCTACA GTCGCTTTTC GGGGGGGCAG ATCGGTGGCA CATTGCTTTG 
TCCTTTTTTG GGCTGCTGGT ACTGCTCTGC TATGCCCCAT TCCGGTGGTA CCCCGAGAGC 
CCCAAGTGGC TGTACATAGT CAAGGGCCGC AAGGAGGAGG CCAGACAACA GCTCCAGCTG 
TTGCGGGGCT ATTCCTCGGA CAGCGCGGCT CTGCAGGCGG AGCTAAACGA AATGGAGCTG 
GAGGCATCGG CCAAGGTGGA GGCTAGTGGA TTCATGGAAG TGCTGCGCAA TCCCCGTCTG 
CGCCTGCCAC TCATCATTGT TTGCGCCTTT CTTGGTGGCC AGCAGCTGTC GGGTATCAAT 
GCGATATTCT TCTACTCTGT CTCCATATTC CGCAAGGCAG GGCTGTCCAC GCAGGCGGCG 
GAGTGGGCCA ATCTGGGTGC TGGATCCCTC AATCTGGCCA CATCGATGGT GGGTCCGGTC 
CTGTTGGAGC GCGTGAATCG TCGCCCGCTG ATGCTCTTCT CCACCTTCTT CTGCGCCGTA 
TGCCTTTTTC TCTTCGCCAT GATGCTCTAC TTCATTGAGA GCTTTAGCTG GTTTGCCATG 
GGCTGCATTG GCTGCATCTT CCTGTACATA TTCTTCTATC AGTTCGGTGT CGGGCCCATG 
CCGTTCTTCA TAGGAGCCGA ACTGTTTGAG GTGTCCCCGC GTCCGGCTGC CATGGCTTTG 
GGTAGCGTTG TCTACTGGGT ATGCAATTTC ATCATTGGCA TGGCCTTTCC CACGCTGCAG 
AATGCCTGGG GCGCCCTGGT CTTCCTCCCA TTCTCCATCG CCTGCTTGCT GCTCTTTGGG 
CTGACCAAAC GCTACTTGCC GGAGACTCGC GGCCGCGATC CATCCGAGGT TGCTCCGCTC 
GTCGCCGACG GCTTTAAATC AAAAGTCTTG CGCACTCGCA CTGCGAGCCA ATCTTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA20656 ( XM_015184750.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040236.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20656), mRNA.

Price & Availability↑

CloneID	ODe23197
Clone ID Related Accession (Same CDS sequence)	XM_015184750.1
Accession Version	XM_015184750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGC CAAAAGCCAG CGAACCCGTG GAGGGGCAAT CATTAAGTCA GGCAGAGGCA 
CCGCTAATGG CACACGAAAA GAAACAGACC CAGGATCAGC CCAAATGGAG CTTTTCCCTC 
AATTTGGCTT GCGTTGGATC CTCGATTGGA GCCGCCTTGC CCGTGGGCTA CTGCACTGGG 
GTCATGAACA GCCCCGCCGT GCTTATGCGC TCCTGGTGCA ACGAAACTCT GATTGCGCGA 
TATGATCTTC ACCTGGGAGC CTCGGGCCTG GAGTGGCTCT GGTCGGCCGT CGTCTCCATC 
TTTCTGGTGG GTGGAGCCAT CGGTTCTATG ACGGGCGCAT CTACCGCCAA CCGCTTTGGA 
CGCCGCGGCT GCTTCTACAT ATGCGGTATG CTACTCGCCC TTGGTGCCGT CTGCTTCGCT 
GTATGCCGCT TGTTGGTATC CGTGGAGCTG CTGCTCCTGG GCCGATTGCT CGTGGGGCTG 
GCGGGGGGCC TCGTCACCGC CTTCATGCCC ATGTACCACA GCGAACTGGC CGCCCTCTCC 
CAGCGAAGTA CTCTGTCTCC GTGTTGCGCA ATGGGCCTGA CCGCAGGCGT GGTGGTAGCC 
CAGATCTGCA GCCTACAGTC GCTTTTCGGG GGGGCAGATC GGTGGCACAT TGCTTTGTCC 
TTTTTTGGGC TGCTGGTACT GCTCTGCTAT GCCCCATTCC GGTGGTACCC CGAGAGCCCC 
AAGTGGCTGT ACATAGTCAA GGGCCGCAAG GAGGAGGCCA GACAACAGCT CCAGCTGTTG 
CGGGGCTATT CCTCGGACAG CGCGGCTCTG CAGGCGGAGC TAAACGAAAT GGAGCTGGAG 
GCATCGGCCA AGGTGGAGGC TAGTGGATTC ATGGAAGTGC TGCGCAATCC CCGTCTGCGC 
CTGCCACTCA TCATTGTTTG CGCCTTTCTT GGTGGCCAGC AGCTGTCGGG TATCAATGCG 
ATATTCTTCT ACTCTGTCTC CATATTCCGC AAGGCAGGGC TGTCCACGCA GGCGGCGGAG 
TGGGCCAATC TGGGTGCTGG ATCCCTCAAT CTGGCCACAT CGATGGTGGG TCCGGTCCTG 
TTGGAGCGCG TGAATCGTCG CCCGCTGATG CTCTTCTCCA CCTTCTTCTG CGCCGTATGC 
CTTTTTCTCT TCGCCATGAT GCTCTACTTC ATTGAGAGCT TTAGCTGGTT TGCCATGGGC 
TGCATTGGCT GCATCTTCCT GTACATATTC TTCTATCAGT TCGGTGTCGG GCCCATGCCG 
TTCTTCATAG GAGCCGAACT GTTTGAGGTG TCCCCGCGTC CGGCTGCCAT GGCTTTGGGT 
AGCGTTGTCT ACTGGGTATG CAATTTCATC ATTGGCATGG CCTTTCCCAC GCTGCAGAAT 
GCCTGGGGCG CCCTGGTCTT CCTCCCATTC TCCATCGCCT GCTTGCTGCT CTTTGGGCTG 
ACCAAACGCT ACTTGCCGGA GACTCGCGGC CGCGATCCAT CCGAGGTTGC TCCGCTCGTC 
GCCGACGGCT TTAAATCAAA AGTCTTGCGC ACTCGCACTG CGAGCCAATC TTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040236.1
CDS	83..1639
Translation	MEKPKASEPVEGQSLSQAEAPLMAHEKKQTQDQPKWSFSLNLACVGSSIGAALPVGYCTGVMNSPAVLMRSWCNETLIARYDLHLGASGLEWLWSAVVSIFLVGGAIGSMTGASTANRFGRRGCFYICGMLLALGAVCFAVCRLLVSVELLLLGRLLVGLAGGLVTAFMPMYHSELAALSQRSTLSPCCAMGLTAGVVVAQICSLQSLFGGADRWHIALSFFGLLVLLCYAPFRWYPESPKWLYIVKGRKEEARQQLQLLRGYSSDSAALQAELNEMELEASAKVEASGFMEVLRNPRLRLPLIIVCAFLGGQQLSGINAIFFYSVSIFRKAGLSTQAAEWANLGAGSLNLATSMVGPVLLERVNRRPLMLFSTFFCAVCLFLFAMMLYFIESFSWFAMGCIGCIFLYIFFYQFGVGPMPFFIGAELFEVSPRPAAMALGSVVYWVCNFIIGMAFPTLQNAWGALVFLPFSIACLLLFGLTKRYLPETRGRDPSEVAPLVADGFKSKVLRTRTASQSS

Target ORF information:

RefSeq Version	XM_015184750.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20656), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015184750.1

ORF Insert Sequence:

ATGGAAAAGC CAAAAGCCAG CGAACCCGTG GAGGGGCAAT CATTAAGTCA GGCAGAGGCA 
CCGCTAATGG CACACGAAAA GAAACAGACC CAGGATCAGC CCAAATGGAG CTTTTCCCTC 
AATTTGGCTT GCGTTGGATC CTCGATTGGA GCCGCCTTGC CCGTGGGCTA CTGCACTGGG 
GTCATGAACA GCCCCGCCGT GCTTATGCGC TCCTGGTGCA ACGAAACTCT GATTGCGCGA 
TATGATCTTC ACCTGGGAGC CTCGGGCCTG GAGTGGCTCT GGTCGGCCGT CGTCTCCATC 
TTTCTGGTGG GTGGAGCCAT CGGTTCTATG ACGGGCGCAT CTACCGCCAA CCGCTTTGGA 
CGCCGCGGCT GCTTCTACAT ATGCGGTATG CTACTCGCCC TTGGTGCCGT CTGCTTCGCT 
GTATGCCGCT TGTTGGTATC CGTGGAGCTG CTGCTCCTGG GCCGATTGCT CGTGGGGCTG 
GCGGGGGGCC TCGTCACCGC CTTCATGCCC ATGTACCACA GCGAACTGGC CGCCCTCTCC 
CAGCGAAGTA CTCTGTCTCC GTGTTGCGCA ATGGGCCTGA CCGCAGGCGT GGTGGTAGCC 
CAGATCTGCA GCCTACAGTC GCTTTTCGGG GGGGCAGATC GGTGGCACAT TGCTTTGTCC 
TTTTTTGGGC TGCTGGTACT GCTCTGCTAT GCCCCATTCC GGTGGTACCC CGAGAGCCCC 
AAGTGGCTGT ACATAGTCAA GGGCCGCAAG GAGGAGGCCA GACAACAGCT CCAGCTGTTG 
CGGGGCTATT CCTCGGACAG CGCGGCTCTG CAGGCGGAGC TAAACGAAAT GGAGCTGGAG 
GCATCGGCCA AGGTGGAGGC TAGTGGATTC ATGGAAGTGC TGCGCAATCC CCGTCTGCGC 
CTGCCACTCA TCATTGTTTG CGCCTTTCTT GGTGGCCAGC AGCTGTCGGG TATCAATGCG 
ATATTCTTCT ACTCTGTCTC CATATTCCGC AAGGCAGGGC TGTCCACGCA GGCGGCGGAG 
TGGGCCAATC TGGGTGCTGG ATCCCTCAAT CTGGCCACAT CGATGGTGGG TCCGGTCCTG 
TTGGAGCGCG TGAATCGTCG CCCGCTGATG CTCTTCTCCA CCTTCTTCTG CGCCGTATGC 
CTTTTTCTCT TCGCCATGAT GCTCTACTTC ATTGAGAGCT TTAGCTGGTT TGCCATGGGC 
TGCATTGGCT GCATCTTCCT GTACATATTC TTCTATCAGT TCGGTGTCGG GCCCATGCCG 
TTCTTCATAG GAGCCGAACT GTTTGAGGTG TCCCCGCGTC CGGCTGCCAT GGCTTTGGGT 
AGCGTTGTCT ACTGGGTATG CAATTTCATC ATTGGCATGG CCTTTCCCAC GCTGCAGAAT 
GCCTGGGGCG CCCTGGTCTT CCTCCCATTC TCCATCGCCT GCTTGCTGCT CTTTGGGCTG 
ACCAAACGCT ACTTGCCGGA GACTCGCGGC CGCGATCCAT CCGAGGTTGC TCCGCTCGTC 
GCCGACGGCT TTAAATCAAA AGTCTTGCGC ACTCGCACTG CGAGCCAATC TTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA20656 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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