Dpse\GA14224 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA14224
Entrez Gene ID	4804702
Full Name	GA14224 gene product from transcript GA14224-RE
Synonyms	GA14224,dpse_GLEANR_15828
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_004444218.2	XP_004444275.2	uncharacterized protein, isoform E
XM_001361183.4	XP_001361220.2	uncharacterized protein, isoform A
XM_004444217.2	XP_004444274.2	uncharacterized protein, isoform F
XM_004444216.2	XP_004444273.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA14224 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA14224 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODe21878	XM_004444218.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA14224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODe03856	XM_001361183.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA14224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODe03857	XM_004444216.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA14224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODe21879	XM_004444217.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA14224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA14224 ( XM_004444218.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444275.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA14224), mRNA.

Price & Availability↑

CloneID	ODe21878
Clone ID Related Accession (Same CDS sequence)	XM_004444218.2
Accession Version	XM_004444218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444218.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTTA ACCAAATTAC GGCCGTGTTG 
GCCAACGTAA GTCTTGATAT ATCCACCACC AACAGCCAGG TGAACAAGAT ACGGGATCAG 
TGCCAGAAGC AGGAGGAGAC CATCAACGAA CAGGAGGGCG AGCTCAATGC CAAGCGCTCC 
GAGCTGCAGA AGCTCAAGGA TGAGGAATCG TCTTTGCAGA AGGAGTACGA CGACAATAAT 
CGCGAGCTCT CCAAGCTGAC CAAGCATCTG CAGAATACGC AGCTCCAAAT TAGCTCGGTG 
CGTTCTATGG TCACTCAATT GATGGAGACC CAGCGCCAGA TGACCGATGC ACTGCTCATC 
TGTCGCGCCG CCATGGAGAC CCAGAACGCG GAGCTAGTCT CCGAGTACCA GCTTAAGATC 
GAGCCGGACT TTGATGACGC TCGCAAGACA CTCCACAAGG AGATCGAGAT GCCCAAGGAC 
GATCCCTTCG ACGAGAACAA CAGCGGAGCG GCCAATCAAG CCAAGCAGTC GACCAACGGC 
TTTGGCAACG ATCCCTTTAG CGGCCAGGTC AGCAAGCCAG CCATCAATGC CGGCTTCGAT 
GACAGCTTCA CGATGAGCTC CGGCTTTGAC AGTGGCTTCG ATGCGTTTGG ACAGAGCGGA 
ACTGGCTCTT CTATGGGCTT TGGCCAGAGC GTAACAGGAT CAGCAACTGG TTTTGGCCAA 
ACGCAACGCG ATCCCTTCGG TGCCGACGCT TTCGGGGGCA GCAAAAGCAG CGCCATAACA 
CCGGAGCCTG GGAAGGACGA CTTTGGAAGC GATCCGTTTG CCTCTCTGCA TGCACCCACT 
GGCCAGGGTC AGGTGCTCAG CCCGAACGCA CAGAGGTCAG GCCCACCACC ACGACCGGAG 
TCGCCTAGTC CAGCATTGCC GCCAAAGAAA TCCAAGATGC CGCCACCCAG ACCAGCCCCG 
CCACGAGCAT CCCAGCCAAC GAGTGGTTTT GGCGGAGGCG GCGGGGGAGG AGGCTTCGCT 
GACTTTGAAG ACTTTGACAA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444275.2
CDS	358..2844
Translation	MNLDFAKVCSKHIGIYEAYYKQIDPKGTGAIEAMTAAKFLKRSGLSDVVLSRIWDLSDPNGKGFLDKPGFFVALKLVSLSQAGQVANMNNIYVDTVNPPKVGELPKTVPARIQTGPVASGGAATGDWTISVIDRLKYEQLFQSLRPHDGMLPGDKVKGVLMESKLPMNILGTIWDLADQDKDGNLDKHEFIVAMHLVYQTLQKRTVPSVLPPELRKPGGTGPPPKPAMPPPPAGAPMPRAPSGEGFGDSGFVANFPKDIAPPAAIPMPIAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVTANADWVVTASELNRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLTANMVPPSMRSTVSGVDMQLQETKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVNQITAVLANVSLDISTTNSQVNKIRDQCQKQEETINEQEGELNAKRSELQKLKDEESSLQKEYDDNNRELSKLTKHLQNTQLQISSVRSMVTQLMETQRQMTDALLICRAAMETQNAELVSEYQLKIEPDFDDARKTLHKEIEMPKDDPFDENNSGAANQAKQSTNGFGNDPFSGQVSKPAINAGFDDSFTMSSGFDSGFDAFGQSGTGSSMGFGQSVTGSATGFGQTQRDPFGADAFGGSKSSAITPEPGKDDFGSDPFASLHAPTGQGQVLSPNAQRSGPPPRPESPSPALPPKKSKMPPPRPAPPRASQPTSGFGGGGGGGGFADFEDFDNK

Target ORF information:

RefSeq Version	XM_004444218.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant E (Dpse\GA14224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444218.2

ORF Insert Sequence:

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTTA ACCAAATTAC GGCCGTGTTG 
GCCAACGTAA GTCTTGATAT ATCCACCACC AACAGCCAGG TGAACAAGAT ACGGGATCAG 
TGCCAGAAGC AGGAGGAGAC CATCAACGAA CAGGAGGGCG AGCTCAATGC CAAGCGCTCC 
GAGCTGCAGA AGCTCAAGGA TGAGGAATCG TCTTTGCAGA AGGAGTACGA CGACAATAAT 
CGCGAGCTCT CCAAGCTGAC CAAGCATCTG CAGAATACGC AGCTCCAAAT TAGCTCGGTG 
CGTTCTATGG TCACTCAATT GATGGAGACC CAGCGCCAGA TGACCGATGC ACTGCTCATC 
TGTCGCGCCG CCATGGAGAC CCAGAACGCG GAGCTAGTCT CCGAGTACCA GCTTAAGATC 
GAGCCGGACT TTGATGACGC TCGCAAGACA CTCCACAAGG AGATCGAGAT GCCCAAGGAC 
GATCCCTTCG ACGAGAACAA CAGCGGAGCG GCCAATCAAG CCAAGCAGTC GACCAACGGC 
TTTGGCAACG ATCCCTTTAG CGGCCAGGTC AGCAAGCCAG CCATCAATGC CGGCTTCGAT 
GACAGCTTCA CGATGAGCTC CGGCTTTGAC AGTGGCTTCG ATGCGTTTGG ACAGAGCGGA 
ACTGGCTCTT CTATGGGCTT TGGCCAGAGC GTAACAGGAT CAGCAACTGG TTTTGGCCAA 
ACGCAACGCG ATCCCTTCGG TGCCGACGCT TTCGGGGGCA GCAAAAGCAG CGCCATAACA 
CCGGAGCCTG GGAAGGACGA CTTTGGAAGC GATCCGTTTG CCTCTCTGCA TGCACCCACT 
GGCCAGGGTC AGGTGCTCAG CCCGAACGCA CAGAGGTCAG GCCCACCACC ACGACCGGAG 
TCGCCTAGTC CAGCATTGCC GCCAAAGAAA TCCAAGATGC CGCCACCCAG ACCAGCCCCG 
CCACGAGCAT CCCAGCCAAC GAGTGGTTTT GGCGGAGGCG GCGGGGGAGG AGGCTTCGCT 
GACTTTGAAG ACTTTGACAA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA14224 ( XM_001361183.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001361220.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA14224), mRNA.

Price & Availability↑

CloneID	ODe03856
Clone ID Related Accession (Same CDS sequence)	XM_001361183.4
Accession Version	XM_001361183.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001361183.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTTA ACCAAATTAC GGCCGTGTTG 
GCCAACGTAA GTCTTGATAT ATCCACCACC AACAGCCAGG TGAACAAGAT ACGGGATCAG 
TGCCAGAAGC AGGAGGAGAC CATCAACGAA CAGGAGGGCG AGCTCAATGC CAAGCGCTCC 
GAGCTGCAGA AGCTCAAGGA TGAGGAATCG TCTTTGCAGA AGGAGTACGA CGACAATAAT 
CGCGAGCTCT CCAAGCTGAC CAAGCATCTG CAGAATACGC AGCTCCAAAT TAGCTCGGTG 
CGTTCTATGG TCACTCAATT GATGGAGACC CAGCGCCAGA TGACCGATGC ACTGCTCATC 
TGTCGCGCCG CCATGGAGAC CCAGAACGCG GAGCTAGTCT CCGAGTACCA GCTTAAGATC 
GAGCCGGACT TTGATGACGC TCGCAAGACA CTCCACAAGG AGATCGAGAT GCCCAAGGAC 
GATCCCTTCG ACGAGAACAA CAGCGGAGCG GCCAATCAAG CCAAGCAGTC GACCAACGGC 
TTTGGCAACG ATCCCTTTAG CGGCCAGGTC AGCAAGCCAG CCATCAATGC CGGCTTCGAT 
GACAGCTTCA CGATGAGCTC CGGCTTTGAC AGTGGCTTCG ATGCGTTTGG ACAGAGCGGA 
ACTGGCTCTT CTATGGGCTT TGGCCAGAGC GTAACAGGAT CAGCAACTGG TTTTGGCCAA 
ACGCAACGCG ATCCCTTCGG TGCCGACGCT TTCGGGGGCA GCAAAAGCAG CGCCATAACA 
CCGGAGCCTG GGAAGGACGA CTTTGGAAGC GATCCGTTTG CCTCTCTGCA TGCACCCACT 
GGCCAGGGTC AGGTGCTCAG CCCGAACGCA CAGAGGTCAG GCCCACCACC ACGACCGGAG 
TCGCCTAGTC CAGCATTGCC GCCAAAGAAA TCCAAGATGC CGCCACCCAG ACCAGCCCCG 
CCACGAGCAT CCCAGCCAAC GAGTGGTTTT GGCGGAGGCG GCGGGGGAGG AGGCTTCGCT 
GACTTTGAAG ACTTTGACAA TAAGCTCCAG AACATTCCCA GCACGCCAGC AAACACGCCC 
ACGCCCATGG CCACGGCCAC CCCTATTCCA ACGATCAATG CCTCGCTGCT GGACAGCTTC 
TCCCTGTTCG ATGATACAGC CTCAGGCTCC AAGGCTGCCA GCACACCTCT GCACCCCTTG 
CTAGCGTCAT CCTCATCCAC GTCAACAGCG TCCACAATTC CCACCGTCAG CAGCACCAGC 
ACCATGTCCA CCACCTCAAA TAACCAACAC TTGTCGCGTT CCAACACACC GCTGCAGACG 
CAAGTCACAA CGGGATTGGG AATCGGAGTA TTAGCAACGA CAGGAAGCCT GGGCCTGGAA 
GCGAGTCTTT TTGATGCCTT TGGCGGCGGC AAAGGAAACG TCAATGCGAA AACACCAACG 
CCAGGCCTCA TCACGGGGCC CACAGACTTT AAGGATGATC CTTTCAAGGA CTACCGCTAT 
GAAGATCCGT TCAGCATCAA AGATCCCTTT GCCGACGATG ATCAGGAGGA GGATGTTGGT 
ACCAAGGGCG AGGCCCATAA AAAGAACTTT GCGGAGGACT TTAGCTCAGA GGATGAGATG 
GGAACCGGCG CCGCCAAGAC CCTCAACAAC ATGAAAGGCA TGAGCATCAA TAACAACACG 
AAACCGAGAG CCGTAACACC CTTGAACAAC GATATCATGC AGCAGTTCGA TGCATTCAAT 
CTGAACGCGA GTCCGGCCCC ATCCTCGCAC CACTCGAATG CCTCACAGAA CCACAAAAAG 
TCCCAGTCGG CGCTGGATGC GTTTGCGGAC TTTGATGACT TTGGATCGGA CACAGCTGGA 
ACTACAGCCA CAACAACAAC AACGACGACA ACCACTAAGA AACTCAGCGA TTCCTTCTTC 
GATGCATTTA ATGACAACTT TAAGCTTAAC GACACCACAA TCACAGCCAC AGGAACAATG 
CCGACATCGG TAACAAAAGC TTTGGCCAGC GAACAAATTA GTACGAGCAG GACGTCATTC 
GATGCCTTTG CCGTTAGTAG CGACGCCTTC AATGACAACT TCGAGGACAG CTTTGGTGGA 
AGCAAGCCGA CCAATTCCAC CCAACTGGAC AACAATTTTG CCAAATTCGA GGCCTTCGAT 
GTCCATAACT TCTCGAGTGA CTTTGGCAAG GGCTTCCCGG AGGGCAAGCA GAATGGCGGG 
GCCAAGAAGG AACAAACTAA GGATAGGTAC GCGGCGGACT ATTCAAAGCC GGAGAGCTTT 
GACGCTGACC TGGAAGAGGC GCTCAAGCGC AGTATGCTGG ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001361220.2
CDS	357..4124
Translation	MNLDFAKVCSKHIGIYEAYYKQIDPKGTGAIEAMTAAKFLKRSGLSDVVLSRIWDLSDPNGKGFLDKPGFFVALKLVSLSQAGQVANMNNIYVDTVNPPKVGELPKTVPARIQTGPVASGGAATGDWTISVIDRLKYEQLFQSLRPHDGMLPGDKVKGVLMESKLPMNILGTIWDLADQDKDGNLDKHEFIVAMHLVYQTLQKRTVPSVLPPELRKPGGTGPPPKPAMPPPPAGAPMPRAPSGEGFGDSGFVANFPKDIAPPAAIPMPIAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVTANADWVVTASELNRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLTANMVPPSMRSTVSGVDMQLQETKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVNQITAVLANVSLDISTTNSQVNKIRDQCQKQEETINEQEGELNAKRSELQKLKDEESSLQKEYDDNNRELSKLTKHLQNTQLQISSVRSMVTQLMETQRQMTDALLICRAAMETQNAELVSEYQLKIEPDFDDARKTLHKEIEMPKDDPFDENNSGAANQAKQSTNGFGNDPFSGQVSKPAINAGFDDSFTMSSGFDSGFDAFGQSGTGSSMGFGQSVTGSATGFGQTQRDPFGADAFGGSKSSAITPEPGKDDFGSDPFASLHAPTGQGQVLSPNAQRSGPPPRPESPSPALPPKKSKMPPPRPAPPRASQPTSGFGGGGGGGGFADFEDFDNKLQNIPSTPANTPTPMATATPIPTINASLLDSFSLFDDTASGSKAASTPLHPLLASSSSTSTASTIPTVSSTSTMSTTSNNQHLSRSNTPLQTQVTTGLGIGVLATTGSLGLEASLFDAFGGGKGNVNAKTPTPGLITGPTDFKDDPFKDYRYEDPFSIKDPFADDDQEEDVGTKGEAHKKNFAEDFSSEDEMGTGAAKTLNNMKGMSINNNTKPRAVTPLNNDIMQQFDAFNLNASPAPSSHHSNASQNHKKSQSALDAFADFDDFGSDTAGTTATTTTTTTTTKKLSDSFFDAFNDNFKLNDTTITATGTMPTSVTKALASEQISTSRTSFDAFAVSSDAFNDNFEDSFGGSKPTNSTQLDNNFAKFEAFDVHNFSSDFGKGFPEGKQNGGAKKEQTKDRYAADYSKPESFDADLEEALKRSMLDN

Target ORF information:

RefSeq Version	XM_001361183.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA14224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001361183.4

ORF Insert Sequence:

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTTA ACCAAATTAC GGCCGTGTTG 
GCCAACGTAA GTCTTGATAT ATCCACCACC AACAGCCAGG TGAACAAGAT ACGGGATCAG 
TGCCAGAAGC AGGAGGAGAC CATCAACGAA CAGGAGGGCG AGCTCAATGC CAAGCGCTCC 
GAGCTGCAGA AGCTCAAGGA TGAGGAATCG TCTTTGCAGA AGGAGTACGA CGACAATAAT 
CGCGAGCTCT CCAAGCTGAC CAAGCATCTG CAGAATACGC AGCTCCAAAT TAGCTCGGTG 
CGTTCTATGG TCACTCAATT GATGGAGACC CAGCGCCAGA TGACCGATGC ACTGCTCATC 
TGTCGCGCCG CCATGGAGAC CCAGAACGCG GAGCTAGTCT CCGAGTACCA GCTTAAGATC 
GAGCCGGACT TTGATGACGC TCGCAAGACA CTCCACAAGG AGATCGAGAT GCCCAAGGAC 
GATCCCTTCG ACGAGAACAA CAGCGGAGCG GCCAATCAAG CCAAGCAGTC GACCAACGGC 
TTTGGCAACG ATCCCTTTAG CGGCCAGGTC AGCAAGCCAG CCATCAATGC CGGCTTCGAT 
GACAGCTTCA CGATGAGCTC CGGCTTTGAC AGTGGCTTCG ATGCGTTTGG ACAGAGCGGA 
ACTGGCTCTT CTATGGGCTT TGGCCAGAGC GTAACAGGAT CAGCAACTGG TTTTGGCCAA 
ACGCAACGCG ATCCCTTCGG TGCCGACGCT TTCGGGGGCA GCAAAAGCAG CGCCATAACA 
CCGGAGCCTG GGAAGGACGA CTTTGGAAGC GATCCGTTTG CCTCTCTGCA TGCACCCACT 
GGCCAGGGTC AGGTGCTCAG CCCGAACGCA CAGAGGTCAG GCCCACCACC ACGACCGGAG 
TCGCCTAGTC CAGCATTGCC GCCAAAGAAA TCCAAGATGC CGCCACCCAG ACCAGCCCCG 
CCACGAGCAT CCCAGCCAAC GAGTGGTTTT GGCGGAGGCG GCGGGGGAGG AGGCTTCGCT 
GACTTTGAAG ACTTTGACAA TAAGCTCCAG AACATTCCCA GCACGCCAGC AAACACGCCC 
ACGCCCATGG CCACGGCCAC CCCTATTCCA ACGATCAATG CCTCGCTGCT GGACAGCTTC 
TCCCTGTTCG ATGATACAGC CTCAGGCTCC AAGGCTGCCA GCACACCTCT GCACCCCTTG 
CTAGCGTCAT CCTCATCCAC GTCAACAGCG TCCACAATTC CCACCGTCAG CAGCACCAGC 
ACCATGTCCA CCACCTCAAA TAACCAACAC TTGTCGCGTT CCAACACACC GCTGCAGACG 
CAAGTCACAA CGGGATTGGG AATCGGAGTA TTAGCAACGA CAGGAAGCCT GGGCCTGGAA 
GCGAGTCTTT TTGATGCCTT TGGCGGCGGC AAAGGAAACG TCAATGCGAA AACACCAACG 
CCAGGCCTCA TCACGGGGCC CACAGACTTT AAGGATGATC CTTTCAAGGA CTACCGCTAT 
GAAGATCCGT TCAGCATCAA AGATCCCTTT GCCGACGATG ATCAGGAGGA GGATGTTGGT 
ACCAAGGGCG AGGCCCATAA AAAGAACTTT GCGGAGGACT TTAGCTCAGA GGATGAGATG 
GGAACCGGCG CCGCCAAGAC CCTCAACAAC ATGAAAGGCA TGAGCATCAA TAACAACACG 
AAACCGAGAG CCGTAACACC CTTGAACAAC GATATCATGC AGCAGTTCGA TGCATTCAAT 
CTGAACGCGA GTCCGGCCCC ATCCTCGCAC CACTCGAATG CCTCACAGAA CCACAAAAAG 
TCCCAGTCGG CGCTGGATGC GTTTGCGGAC TTTGATGACT TTGGATCGGA CACAGCTGGA 
ACTACAGCCA CAACAACAAC AACGACGACA ACCACTAAGA AACTCAGCGA TTCCTTCTTC 
GATGCATTTA ATGACAACTT TAAGCTTAAC GACACCACAA TCACAGCCAC AGGAACAATG 
CCGACATCGG TAACAAAAGC TTTGGCCAGC GAACAAATTA GTACGAGCAG GACGTCATTC 
GATGCCTTTG CCGTTAGTAG CGACGCCTTC AATGACAACT TCGAGGACAG CTTTGGTGGA 
AGCAAGCCGA CCAATTCCAC CCAACTGGAC AACAATTTTG CCAAATTCGA GGCCTTCGAT 
GTCCATAACT TCTCGAGTGA CTTTGGCAAG GGCTTCCCGG AGGGCAAGCA GAATGGCGGG 
GCCAAGAAGG AACAAACTAA GGATAGGTAC GCGGCGGACT ATTCAAAGCC GGAGAGCTTT 
GACGCTGACC TGGAAGAGGC GCTCAAGCGC AGTATGCTGG ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA14224 ( XM_004444216.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444273.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA14224), mRNA.

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CloneID	ODe03857
Clone ID Related Accession (Same CDS sequence)	XM_004444216.2
Accession Version	XM_004444216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444216.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTGA ACAAGATACG GGATCAGTGC 
CAGAAGCAGG AGGAGACCAT CAACGAACAG GAGGGCGAGC TCAATGCCAA GCGCTCCGAG 
CTGCAGAAGC TCAAGGATGA GGAATCGTCT TTGCAGAAGG AGTACGACGA CAATAATCGC 
GAGCTCTCCA AGCTGACCAA GCATCTGCAG AATACGCAGC TCCAAATTAG CTCGGTGCGT 
TCTATGGTCA CTCAATTGAT GGAGACCCAG CGCCAGATGA CCGATGCACT GCTCATCTGT 
CGCGCCGCCA TGGAGACCCA GAACGCGGAG CTAGTCTCCG AGTACCAGCT TAAGATCGAG 
CCGGACTTTG ATGACGCTCG CAAGACACTC CACAAGGAGA TCGAGATGCC CAAGGACGAT 
CCCTTCGACG AGAACAACAG CGGAGCGGCC AATCAAGCCA AGCAGTCGAC CAACGGCTTT 
GGCAACGATC CCTTTAGCGG CCAGGTCAGC AAGCCAGCCA TCAATGCCGG CTTCGATGAC 
AGCTTCACGA TGAGCTCCGG CTTTGACAGT GGCTTCGATG CGTTTGGACA GAGCGGAACT 
GGCTCTTCTA TGGGCTTTGG CCAGAGCGTA ACAGGATCAG CAACTGGTTT TGGCCAAACG 
CAACGCGATC CCTTCGGTGC CGACGCTTTC GGGGGCAGCA AAAGCAGCGC CATAACACCG 
GAGCCTGGGA AGGACGACTT TGGAAGCGAT CCGTTTGCCT CTCTGCATGC ACCCACTGGC 
CAGGGTCAGG TGCTCAGCCC GAACGCACAG AGGTCAGGCC CACCACCACG ACCGGAGTCG 
CCTAGTCCAG CATTGCCGCC AAAGAAATCC AAGATGCCGC CACCCAGACC AGCCCCGCCA 
CGAGCATCCC AGCCAACGAG TGGTTTTGGC GGAGGCGGCG GGGGAGGAGG CTTCGCTGAC 
TTTGAAGACT TTGACAATAA GCTCCAGAAC ATTCCCAGCA CGCCAGCAAA CACGCCCACG 
CCCATGGCCA CGGCCACCCC TATTCCAACG ATCAATGCCT CGCTGCTGGA CAGCTTCTCC 
CTGTTCGATG ATACAGCCTC AGGCTCCAAG GCTGCCAGCA CACCTCTGCA CCCCTTGCTA 
GCGTCATCCT CATCCACGTC AACAGCGTCC ACAATTCCCA CCGTCAGCAG CACCAGCACC 
ATGTCCACCA CCTCAAATAA CCAACACTTG TCGCGTTCCA ACACACCGCT GCAGACGCAA 
GTCACAACGG GATTGGGAAT CGGAGTATTA GCAACGACAG GAAGCCTGGG CCTGGAAGCG 
AGTCTTTTTG ATGCCTTTGG CGGCGGCAAA GGAAACGTCA ATGCGAAAAC ACCAACGCCA 
GGCCTCATCA CGGGGCCCAC AGACTTTAAG GATGATCCTT TCAAGGACTA CCGCTATGAA 
GATCCGTTCA GCATCAAAGA TCCCTTTGCC GACGATGATC AGGAGGAGGA TGTTGGTACC 
AAGGGCGAGG CCCATAAAAA GAACTTTGCG GAGGACTTTA GCTCAGAGGA TGAGATGGGA 
ACCGGCGCCG CCAAGACCCT CAACAACATG AAAGGCATGA GCATCAATAA CAACACGAAA 
CCGAGAGCCG TAACACCCTT GAACAACGAT ATCATGCAGC AGTTCGATGC ATTCAATCTG 
AACGCGAGTC CGGCCCCATC CTCGCACCAC TCGAATGCCT CACAGAACCA CAAAAAGTCC 
CAGTCGGCGC TGGATGCGTT TGCGGACTTT GATGACTTTG GATCGGACAC AGCTGGAACT 
ACAGCCACAA CAACAACAAC GACGACAACC ACTAAGAAAC TCAGCGATTC CTTCTTCGAT 
GCATTTAATG ACAACTTTAA GCTTAACGAC ACCACAATCA CAGCCACAGG AACAATGCCG 
ACATCGGTAA CAAAAGCTTT GGCCAGCGAA CAAATTAGTA CGAGCAGGAC GTCATTCGAT 
GCCTTTGCCG TTAGTAGCGA CGCCTTCAAT GACAACTTCG AGGACAGCTT TGGTGGAAGC 
AAGCCGACCA ATTCCACCCA ACTGGACAAC AATTTTGCCA AATTCGAGGC CTTCGATGTC 
CATAACTTCT CGAGTGACTT TGGCAAGGGC TTCCCGGAGG GCAAGCAGAA TGGCGGGGCC 
AAGAAGGAAC AAACTAAGGA TAGGTACGCG GCGGACTATT CAAAGCCGGA GAGCTTTGAC 
GCTGACCTGG AAGAGGCGCT CAAGCGCAGT ATGCTGGACA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444273.1
CDS	357..4061
Translation	MNLDFAKVCSKHIGIYEAYYKQIDPKGTGAIEAMTAAKFLKRSGLSDVVLSRIWDLSDPNGKGFLDKPGFFVALKLVSLSQAGQVANMNNIYVDTVNPPKVGELPKTVPARIQTGPVASGGAATGDWTISVIDRLKYEQLFQSLRPHDGMLPGDKVKGVLMESKLPMNILGTIWDLADQDKDGNLDKHEFIVAMHLVYQTLQKRTVPSVLPPELRKPGGTGPPPKPAMPPPPAGAPMPRAPSGEGFGDSGFVANFPKDIAPPAAIPMPIAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVTANADWVVTASELNRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLTANMVPPSMRSTVSGVDMQLQETKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVNKIRDQCQKQEETINEQEGELNAKRSELQKLKDEESSLQKEYDDNNRELSKLTKHLQNTQLQISSVRSMVTQLMETQRQMTDALLICRAAMETQNAELVSEYQLKIEPDFDDARKTLHKEIEMPKDDPFDENNSGAANQAKQSTNGFGNDPFSGQVSKPAINAGFDDSFTMSSGFDSGFDAFGQSGTGSSMGFGQSVTGSATGFGQTQRDPFGADAFGGSKSSAITPEPGKDDFGSDPFASLHAPTGQGQVLSPNAQRSGPPPRPESPSPALPPKKSKMPPPRPAPPRASQPTSGFGGGGGGGGFADFEDFDNKLQNIPSTPANTPTPMATATPIPTINASLLDSFSLFDDTASGSKAASTPLHPLLASSSSTSTASTIPTVSSTSTMSTTSNNQHLSRSNTPLQTQVTTGLGIGVLATTGSLGLEASLFDAFGGGKGNVNAKTPTPGLITGPTDFKDDPFKDYRYEDPFSIKDPFADDDQEEDVGTKGEAHKKNFAEDFSSEDEMGTGAAKTLNNMKGMSINNNTKPRAVTPLNNDIMQQFDAFNLNASPAPSSHHSNASQNHKKSQSALDAFADFDDFGSDTAGTTATTTTTTTTTKKLSDSFFDAFNDNFKLNDTTITATGTMPTSVTKALASEQISTSRTSFDAFAVSSDAFNDNFEDSFGGSKPTNSTQLDNNFAKFEAFDVHNFSSDFGKGFPEGKQNGGAKKEQTKDRYAADYSKPESFDADLEEALKRSMLDN

Target ORF information:

RefSeq Version	XM_004444216.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA14224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444216.2

ORF Insert Sequence:

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTGA ACAAGATACG GGATCAGTGC 
CAGAAGCAGG AGGAGACCAT CAACGAACAG GAGGGCGAGC TCAATGCCAA GCGCTCCGAG 
CTGCAGAAGC TCAAGGATGA GGAATCGTCT TTGCAGAAGG AGTACGACGA CAATAATCGC 
GAGCTCTCCA AGCTGACCAA GCATCTGCAG AATACGCAGC TCCAAATTAG CTCGGTGCGT 
TCTATGGTCA CTCAATTGAT GGAGACCCAG CGCCAGATGA CCGATGCACT GCTCATCTGT 
CGCGCCGCCA TGGAGACCCA GAACGCGGAG CTAGTCTCCG AGTACCAGCT TAAGATCGAG 
CCGGACTTTG ATGACGCTCG CAAGACACTC CACAAGGAGA TCGAGATGCC CAAGGACGAT 
CCCTTCGACG AGAACAACAG CGGAGCGGCC AATCAAGCCA AGCAGTCGAC CAACGGCTTT 
GGCAACGATC CCTTTAGCGG CCAGGTCAGC AAGCCAGCCA TCAATGCCGG CTTCGATGAC 
AGCTTCACGA TGAGCTCCGG CTTTGACAGT GGCTTCGATG CGTTTGGACA GAGCGGAACT 
GGCTCTTCTA TGGGCTTTGG CCAGAGCGTA ACAGGATCAG CAACTGGTTT TGGCCAAACG 
CAACGCGATC CCTTCGGTGC CGACGCTTTC GGGGGCAGCA AAAGCAGCGC CATAACACCG 
GAGCCTGGGA AGGACGACTT TGGAAGCGAT CCGTTTGCCT CTCTGCATGC ACCCACTGGC 
CAGGGTCAGG TGCTCAGCCC GAACGCACAG AGGTCAGGCC CACCACCACG ACCGGAGTCG 
CCTAGTCCAG CATTGCCGCC AAAGAAATCC AAGATGCCGC CACCCAGACC AGCCCCGCCA 
CGAGCATCCC AGCCAACGAG TGGTTTTGGC GGAGGCGGCG GGGGAGGAGG CTTCGCTGAC 
TTTGAAGACT TTGACAATAA GCTCCAGAAC ATTCCCAGCA CGCCAGCAAA CACGCCCACG 
CCCATGGCCA CGGCCACCCC TATTCCAACG ATCAATGCCT CGCTGCTGGA CAGCTTCTCC 
CTGTTCGATG ATACAGCCTC AGGCTCCAAG GCTGCCAGCA CACCTCTGCA CCCCTTGCTA 
GCGTCATCCT CATCCACGTC AACAGCGTCC ACAATTCCCA CCGTCAGCAG CACCAGCACC 
ATGTCCACCA CCTCAAATAA CCAACACTTG TCGCGTTCCA ACACACCGCT GCAGACGCAA 
GTCACAACGG GATTGGGAAT CGGAGTATTA GCAACGACAG GAAGCCTGGG CCTGGAAGCG 
AGTCTTTTTG ATGCCTTTGG CGGCGGCAAA GGAAACGTCA ATGCGAAAAC ACCAACGCCA 
GGCCTCATCA CGGGGCCCAC AGACTTTAAG GATGATCCTT TCAAGGACTA CCGCTATGAA 
GATCCGTTCA GCATCAAAGA TCCCTTTGCC GACGATGATC AGGAGGAGGA TGTTGGTACC 
AAGGGCGAGG CCCATAAAAA GAACTTTGCG GAGGACTTTA GCTCAGAGGA TGAGATGGGA 
ACCGGCGCCG CCAAGACCCT CAACAACATG AAAGGCATGA GCATCAATAA CAACACGAAA 
CCGAGAGCCG TAACACCCTT GAACAACGAT ATCATGCAGC AGTTCGATGC ATTCAATCTG 
AACGCGAGTC CGGCCCCATC CTCGCACCAC TCGAATGCCT CACAGAACCA CAAAAAGTCC 
CAGTCGGCGC TGGATGCGTT TGCGGACTTT GATGACTTTG GATCGGACAC AGCTGGAACT 
ACAGCCACAA CAACAACAAC GACGACAACC ACTAAGAAAC TCAGCGATTC CTTCTTCGAT 
GCATTTAATG ACAACTTTAA GCTTAACGAC ACCACAATCA CAGCCACAGG AACAATGCCG 
ACATCGGTAA CAAAAGCTTT GGCCAGCGAA CAAATTAGTA CGAGCAGGAC GTCATTCGAT 
GCCTTTGCCG TTAGTAGCGA CGCCTTCAAT GACAACTTCG AGGACAGCTT TGGTGGAAGC 
AAGCCGACCA ATTCCACCCA ACTGGACAAC AATTTTGCCA AATTCGAGGC CTTCGATGTC 
CATAACTTCT CGAGTGACTT TGGCAAGGGC TTCCCGGAGG GCAAGCAGAA TGGCGGGGCC 
AAGAAGGAAC AAACTAAGGA TAGGTACGCG GCGGACTATT CAAAGCCGGA GAGCTTTGAC 
GCTGACCTGG AAGAGGCGCT CAAGCGCAGT ATGCTGGACA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA14224 ( XM_004444217.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444274.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA14224), mRNA.

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CloneID	ODe21879
Clone ID Related Accession (Same CDS sequence)	XM_004444217.2
Accession Version	XM_004444217.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444217.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTTA ACCAAATTAC GGCCGTGTTG 
GCCAACGTAA GTCTTGATAT ATCCACCACC AACAGCCAGG TGAACAAGAT ACGGGATCAG 
TGCCAGAAGC AGGAGGAGAC CATCAACGAA CAGGAGGGCG AGCTCAATGC CAAGCGCTCC 
GAGCTGCAGA AGCTCAAGGA TGAGGAATCG TCTTTGCAGA AGGAGTACGA CGACAATAAT 
CGCGAGCTCT CCAAGCTGAC CAAGCATCTG CAGAATACGC AGCTCCAAAT TAGCTCGGTG 
CGTTCTATGG TCACTCAATT GATGGAGACC CAGCGCCAGA TGACCGATGC ACTGCTCATC 
TGTCGCGCCG CCATGGAGAC CCAGAACGCG GAGCTAGTCT CCGAGTACCA GCTTAAGATC 
GAGCCGGACT TTGATGACGC TCGCAAGACA CTCCACAAGG AGATCGAGAT GCCCAAGGAC 
GATCCCTTCG ACGAGAACAA CAGCGGAGCG GCCAATCAAG CCAAGCAGTC GACCAACGGC 
TTTGGCAACG ATCCCTTTAG CGGCCAGGTC AGCAAGCCAG CCATCAATGC CGGCTTCGAT 
GACAGCTTCA CGATGAGCTC CGGCTTTGAC AGTGGCTTCG ATGCGTTTGG ACAGAGCGGA 
ACTGGCTCTT CTATGGGCTT TGGCCAGAGC GTAACAGGAT CAGCAACTGG TTTTGGCCAA 
ACGCAACGCG ATCCCTTCGG TGCCGACGCT TTCGGGGGCA GCAAAAGCAG CGCCATAACA 
CCGGAGCCTG GGAAGGACGA CTTTGGAAGC GATCCGTTTG CCTCTCTGCA TGCACCCACT 
GGCCAGGGTC AGGTGCTCAG CCCGAACGCA CAGAGGTCAG GCCCACCACC ACGACCGGAG 
TCGCCTAGTC CAGCATTGCC GCCAAAGAAA TCCAAGATGC CGCCACCCAG ACCAGCCCCG 
CCACGAGCAT CCCAGCCAAC GAGTGGTTTT GGCGGAGGCG GCGGGGGAGG AGGCTTCGCT 
GACTTTGAAG ACTTTGACAA TAAGACGCAA GTCACAACGG GATTGGGAAT CGGAGTATTA 
GCAACGACAG GAAGCCTGGG CCTGGAAGCG AGTCTTTTTG ATGCCTTTGG CGGCGGCAAA 
GGAAACGTCA ATGCGAAAAC ACCAACGCCA GGCCTCATCA CGGGGCCCAC AGACTTTAAG 
GATGATCCTT TCAAGGACTA CCGCTATGAA GATCCGTTCA GCATCAAAGA TCCCTTTGCC 
GACGATGATC AGGAGGAGGA TGTTGGTACC AAGGGCGAGG CCCATAAAAA GAACTTTGCG 
GAGGACTTTA GCTCAGAGGA TGAGATGGGA ACCGGCGCCG CCAAGACCCT CAACAACATG 
AAAGGCATGA GCATCAATAA CAACACGAAA CCGAGAGCCG TAACACCCTT GAACAACGAT 
ATCATGCAGC AGTTCGATGC ATTCAATCTG AACGCGAGTC CGGCCCCATC CTCGCACCAC 
TCGAATGCCT CACAGAACCA CAAAAAGTCC CAGTCGGCGC TGGATGCGTT TGCGGACTTT 
GATGACTTTG GATCGGACAC AGCTGGAACT ACAGCCACAA CAACAACAAC GACGACAACC 
ACTAAGAAAC TCAGCGATTC CTTCTTCGAT GCATTTAATG ACAACTTTAA GCTTAACGAC 
ACCACAATCA CAGCCACAGG AACAATGCCG ACATCGGTAA CAAAAGCTTT GGCCAGCGAA 
CAAATTAGTA CGAGCAGGAC GTCATTCGAT GCCTTTGCCG TTAGTAGCGA CGCCTTCAAT 
GACAACTTCG AGGACAGCTT TGGTGGAAGC AAGCCGACCA ATTCCACCCA ACTGGACAAC 
AATTTTGCCA AATTCGAGGC CTTCGATGTC CATAACTTCT CGAGTGACTT TGGCAAGGGC 
TTCCCGGAGG GCAAGCAGAA TGGCGGGGCC AAGAAGGAAC AAACTAAGGA TAGGTACGCG 
GCGGACTATT CAAAGCCGGA GAGCTTTGAC GCTGACCTGG AAGAGGCGCT CAAGCGCAGT 
ATGCTGGACA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444274.2
CDS	357..3851
Translation	MNLDFAKVCSKHIGIYEAYYKQIDPKGTGAIEAMTAAKFLKRSGLSDVVLSRIWDLSDPNGKGFLDKPGFFVALKLVSLSQAGQVANMNNIYVDTVNPPKVGELPKTVPARIQTGPVASGGAATGDWTISVIDRLKYEQLFQSLRPHDGMLPGDKVKGVLMESKLPMNILGTIWDLADQDKDGNLDKHEFIVAMHLVYQTLQKRTVPSVLPPELRKPGGTGPPPKPAMPPPPAGAPMPRAPSGEGFGDSGFVANFPKDIAPPAAIPMPIAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVTANADWVVTASELNRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLTANMVPPSMRSTVSGVDMQLQETKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVNQITAVLANVSLDISTTNSQVNKIRDQCQKQEETINEQEGELNAKRSELQKLKDEESSLQKEYDDNNRELSKLTKHLQNTQLQISSVRSMVTQLMETQRQMTDALLICRAAMETQNAELVSEYQLKIEPDFDDARKTLHKEIEMPKDDPFDENNSGAANQAKQSTNGFGNDPFSGQVSKPAINAGFDDSFTMSSGFDSGFDAFGQSGTGSSMGFGQSVTGSATGFGQTQRDPFGADAFGGSKSSAITPEPGKDDFGSDPFASLHAPTGQGQVLSPNAQRSGPPPRPESPSPALPPKKSKMPPPRPAPPRASQPTSGFGGGGGGGGFADFEDFDNKTQVTTGLGIGVLATTGSLGLEASLFDAFGGGKGNVNAKTPTPGLITGPTDFKDDPFKDYRYEDPFSIKDPFADDDQEEDVGTKGEAHKKNFAEDFSSEDEMGTGAAKTLNNMKGMSINNNTKPRAVTPLNNDIMQQFDAFNLNASPAPSSHHSNASQNHKKSQSALDAFADFDDFGSDTAGTTATTTTTTTTTKKLSDSFFDAFNDNFKLNDTTITATGTMPTSVTKALASEQISTSRTSFDAFAVSSDAFNDNFEDSFGGSKPTNSTQLDNNFAKFEAFDVHNFSSDFGKGFPEGKQNGGAKKEQTKDRYAADYSKPESFDADLEEALKRSMLDN

Target ORF information:

RefSeq Version	XM_004444217.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant F (Dpse\GA14224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444217.2

ORF Insert Sequence:

ATGAATTTGG ATTTTGCCAA GGTGTGCAGT AAGCACATCG GCATCTACGA GGCTTACTAT 
AAACAGATTG ACCCGAAAGG CACTGGAGCC ATCGAGGCCA TGACGGCGGC CAAGTTTCTC 
AAGAGGTCCG GCCTCAGCGA TGTGGTGCTC AGTCGGATCT GGGATCTGTC CGATCCAAAT 
GGGAAGGGCT TCCTTGACAA GCCCGGTTTC TTTGTGGCCC TGAAGCTAGT CTCGCTCTCC 
CAGGCGGGAC AGGTGGCCAA CATGAACAAC ATCTATGTGG ACACTGTCAA CCCTCCGAAA 
GTGGGTGAAT TACCAAAAAC GGTGCCCGCT CGCATTCAAA CAGGTCCCGT CGCGAGCGGA 
GGCGCGGCCA CCGGCGACTG GACAATCAGT GTCATCGATC GGCTTAAATA CGAGCAGCTT 
TTCCAGTCGC TCCGTCCACA CGACGGCATG CTGCCCGGCG ACAAGGTGAA AGGCGTGCTT 
ATGGAGTCCA AGCTACCGAT GAACATCCTC GGCACCATCT GGGACCTGGC TGATCAGGAC 
AAGGACGGCA ATTTGGACAA GCACGAATTT ATCGTGGCCA TGCATCTCGT CTATCAGACG 
CTCCAGAAGC GCACCGTGCC CAGTGTCCTA CCACCCGAGC TACGGAAGCC CGGTGGCACT 
GGGCCGCCTC CCAAACCTGC CATGCCTCCA CCACCAGCTG GCGCTCCCAT GCCGCGTGCC 
CCCAGCGGTG AGGGGTTCGG CGATAGTGGC TTCGTCGCCA ACTTCCCCAA GGACATTGCC 
CCGCCGGCGG CTATTCCTAT GCCCATAGCC GTGCCGCCCA TGACTCGCAT TCCGCCCGTG 
GGCGCGGTCA GTTCCCAGCC TCTGATCCAG ACCGATCCTC TCATACCAAT CGGCGCCCCG 
GTGACGGCCA ATGCAGACTG GGTGGTGACT GCCTCCGAGC TGAACCGATT CGAGGAGATA 
TTCCGACAGT CGGACCTGGA CAAGGACGGC CTGGTCTCTG GCCTCGAGGT GAAGGACATT 
TTCATAAAGT CGGGTATACC ACAACGCAGT CTGGCAGACA TCTGGGCGCT GTGCGACACC 
AATCAGTCGG GTAAGTTGAC TGTGGAGCAG TTCGCCCTGG CCATGTGGTT CGTCGAGCGG 
AAGCAGCGTG GCGTGGATCC GCCGCATGTT CTGACCGCCA ACATGGTGCC GCCATCAATG 
CGCTCGACTG TATCGGGCGT GGACATGCAG CTGCAGGAGA CGAAGCCCAC ATACTCCAAT 
CCCGAGCTGG AGATGATCTC CAAGGAGATC GAGGAGCTGG CTCGTGAAAG ACGTGTGCTC 
GAGACGGAGA TCGCCCAAAA GGAAGCTGAC GTGCGCATCA AGAACGGGGA AGTCCGCAGC 
CTGCAGAGCG AACTGGACAC CTTGACGGCC ACACTGAAGC AACTGGAGAA TCAGCGGGGA 
GAGGCCCAGA AGCGTCTGGA CGATCTGCAG GCTCAAGTTA ACCAAATTAC GGCCGTGTTG 
GCCAACGTAA GTCTTGATAT ATCCACCACC AACAGCCAGG TGAACAAGAT ACGGGATCAG 
TGCCAGAAGC AGGAGGAGAC CATCAACGAA CAGGAGGGCG AGCTCAATGC CAAGCGCTCC 
GAGCTGCAGA AGCTCAAGGA TGAGGAATCG TCTTTGCAGA AGGAGTACGA CGACAATAAT 
CGCGAGCTCT CCAAGCTGAC CAAGCATCTG CAGAATACGC AGCTCCAAAT TAGCTCGGTG 
CGTTCTATGG TCACTCAATT GATGGAGACC CAGCGCCAGA TGACCGATGC ACTGCTCATC 
TGTCGCGCCG CCATGGAGAC CCAGAACGCG GAGCTAGTCT CCGAGTACCA GCTTAAGATC 
GAGCCGGACT TTGATGACGC TCGCAAGACA CTCCACAAGG AGATCGAGAT GCCCAAGGAC 
GATCCCTTCG ACGAGAACAA CAGCGGAGCG GCCAATCAAG CCAAGCAGTC GACCAACGGC 
TTTGGCAACG ATCCCTTTAG CGGCCAGGTC AGCAAGCCAG CCATCAATGC CGGCTTCGAT 
GACAGCTTCA CGATGAGCTC CGGCTTTGAC AGTGGCTTCG ATGCGTTTGG ACAGAGCGGA 
ACTGGCTCTT CTATGGGCTT TGGCCAGAGC GTAACAGGAT CAGCAACTGG TTTTGGCCAA 
ACGCAACGCG ATCCCTTCGG TGCCGACGCT TTCGGGGGCA GCAAAAGCAG CGCCATAACA 
CCGGAGCCTG GGAAGGACGA CTTTGGAAGC GATCCGTTTG CCTCTCTGCA TGCACCCACT 
GGCCAGGGTC AGGTGCTCAG CCCGAACGCA CAGAGGTCAG GCCCACCACC ACGACCGGAG 
TCGCCTAGTC CAGCATTGCC GCCAAAGAAA TCCAAGATGC CGCCACCCAG ACCAGCCCCG 
CCACGAGCAT CCCAGCCAAC GAGTGGTTTT GGCGGAGGCG GCGGGGGAGG AGGCTTCGCT 
GACTTTGAAG ACTTTGACAA TAAGACGCAA GTCACAACGG GATTGGGAAT CGGAGTATTA 
GCAACGACAG GAAGCCTGGG CCTGGAAGCG AGTCTTTTTG ATGCCTTTGG CGGCGGCAAA 
GGAAACGTCA ATGCGAAAAC ACCAACGCCA GGCCTCATCA CGGGGCCCAC AGACTTTAAG 
GATGATCCTT TCAAGGACTA CCGCTATGAA GATCCGTTCA GCATCAAAGA TCCCTTTGCC 
GACGATGATC AGGAGGAGGA TGTTGGTACC AAGGGCGAGG CCCATAAAAA GAACTTTGCG 
GAGGACTTTA GCTCAGAGGA TGAGATGGGA ACCGGCGCCG CCAAGACCCT CAACAACATG 
AAAGGCATGA GCATCAATAA CAACACGAAA CCGAGAGCCG TAACACCCTT GAACAACGAT 
ATCATGCAGC AGTTCGATGC ATTCAATCTG AACGCGAGTC CGGCCCCATC CTCGCACCAC 
TCGAATGCCT CACAGAACCA CAAAAAGTCC CAGTCGGCGC TGGATGCGTT TGCGGACTTT 
GATGACTTTG GATCGGACAC AGCTGGAACT ACAGCCACAA CAACAACAAC GACGACAACC 
ACTAAGAAAC TCAGCGATTC CTTCTTCGAT GCATTTAATG ACAACTTTAA GCTTAACGAC 
ACCACAATCA CAGCCACAGG AACAATGCCG ACATCGGTAA CAAAAGCTTT GGCCAGCGAA 
CAAATTAGTA CGAGCAGGAC GTCATTCGAT GCCTTTGCCG TTAGTAGCGA CGCCTTCAAT 
GACAACTTCG AGGACAGCTT TGGTGGAAGC AAGCCGACCA ATTCCACCCA ACTGGACAAC 
AATTTTGCCA AATTCGAGGC CTTCGATGTC CATAACTTCT CGAGTGACTT TGGCAAGGGC 
TTCCCGGAGG GCAAGCAGAA TGGCGGGGCC AAGAAGGAAC AAACTAAGGA TAGGTACGCG 
GCGGACTATT CAAAGCCGGA GAGCTTTGAC GCTGACCTGG AAGAGGCGCT CAAGCGCAGT 
ATGCTGGACA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA14224 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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