Dpse\GA20741 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA20741
Entrez Gene ID	4803182
Full Name	GA20741 gene product from transcript GA20741-RB
Synonyms	GA20741,dpse_GLEANR_6437
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015182852.1	XP_015038338.1	uncharacterized protein, isoform B
XM_001359928.3	XP_001359965.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA20741 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA20741 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe02582	XM_001359928.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20741), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODe20957	XM_015182852.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20741), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA20741 ( XM_001359928.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001359965.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20741), mRNA.

Price & Availability↑

CloneID	ODe02582
Clone ID Related Accession (Same CDS sequence)	XM_001359928.3
Accession Version	XM_001359928.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001359928.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACTCGA ATCTGTGGAT ATTTATTTGG CGCCGCAAAC TCTCCTTCCT GCTAGTAGTG 
TCTGGCCTCG TGCTGCTGGG TCTCTGGACA TGGGCCATAC TCATCGATAC GACCACCACG 
CCACCACTGC CGGTTCCCTC AGCGAATGCT GAGGCAAGAG AGCGCCAACA GCAGCAGCAG 
AACCCGGGAT ACCAGCAGGT CCATATTATC CAGAAGCTAA TTGCCCAGGC CAACCGTGTG 
CTCCGCGCCG ATCGACTCAA GGACAACGCC GCCGGAGAGA AGGCGCCAAC TGTCCAGCGT 
CTGGGCAACG TGCGATTCCT GCGTCCCACC CAGGCCAAGA AGGCCCCACT GCCGCTGTCC 
GAGAGCTATC TGTTCGAGCA GGAGCGCCTG AAGATACTCG AACAGCAGCA GCGTCAGCGC 
AACGCCGGAA TGGAGGAGCT GAATGCCAAT GCCGAGGATG AGCGTATGCT CAGCTCTGCC 
GAGCGCCAGA CCCAGTACGA GCACGATCTC CAGCGGATGG GCGTTCCCGT GGTGGCTGGT 
ATCGGACCAG ACGGGCCACG TTCCCCTAAC GAGCGTCTCG TCCACTTGGA TCTGAAGGGG 
GCCCCGCCCA AGCTGAGTTT CCTCAAGCAA CTGCTGCCGA TGCTCAGGGC TCTGGGTGCC 
ACGGGGCTAC TGATCGAATA CGAGGATATG TTTCCCTTTA CTGGAGCCCT CCAGCCGCTG 
GCCGCCCACA ATGCCTACAA AGAAGATGAA TTGAGGGACT TTCTGGAGTG CGCCGCCTTG 
CATGGTTTGA GTGTGATGCC TCTGCTTCAG ACCTTCGGGC ATTTGGAGTA TGTCCTGAAA 
CTGGAGGGAT TCGAGCAGCT GCGAGAGCTG CCCGAAAGTC CCCAATCCAT CTGCCCCAGT 
CAGCCGCAGA GCATGGCCCT GCTGGAGCAG ATGCTCACCC AGGTGATCGA GCTGCATTTA 
CAGTGCATCG GCAAGGCCAC CCCCTCAGCC CCGACACTGC CCATCAAGTT CACGCACATC 
CACATCGGAT GCGATGAGGT GCAGCGCATG GGGGAGTGCC AGGCCTGCCG CCAGAGACTC 
CGGAGCGAGC TGTTCCTGTC GCACGTGATC AGTCTCTCGC ACTTTGTGCG TCGCCACTGG 
CCCCAACTGG GCGTGGTCAT CTGGGATGAT CAGCTGCGAT CGATGTCACT CAGCGAGCTG 
CAACACTCGC AGATCGGCAG CTATGTGGAG CCCATGGTGT GGGTGTATGC CAGCGATATC 
TATCGGTTCA TCCAGCCGCA GCTGTGGGAC ACCTATGCCA AGGTCTTTCC CAGTGCTTGG 
GTGGCCTCGG CCTACAAGGG AGCCTTCGGC GAGAGCCTGC TGGTGCCGCC GCTGCAGCAT 
CATCTGGAAA ATAACATACG CTGGCTGGCG GTCATCGCCA AGGAGGGCGG ACGCTTTGCC 
AAAGGACTGC GCGGTGTGGC TCTAACCGGC TGGCAGCGAT ACGATCACTT TGCCGTCCTG 
TGCGAACTGC TGCCAGTGGG AATGCCCAGC CTGATGGTGT CGCTGTCGAC TGTTTCCAAG 
GGTTATTTCA GCACTAATCC AAGGGACAAC GATCTGCTGC GCGTGCTGCA GTGCGTCTTT 
CAGCCGGACA GCCGGCGCTC GGGTCGCCCC TGGCTGGAGC TTCATCCGAA TGCCCATCAT 
AGCCAACTGT TTGCTGTCTG CAACTATCCC GGCCACATGG TCTTCAAGTA TGCCCTTCGC 
CTGTACGACA AACTGGCCGA AGTGGGAGCC TATCTACAGC AGACGCGCGA GCAGAGCGCC 
TGGCTCTCCG ACTACAATGT GAGGCACAAC TTCAGCTCCC CGTTGAGAGT TCACGAGCTG 
ATTGCCCGAA CGCCAATACT AATGGAGGAG CTGCGCGCAA TGGCTCGCGA GGCACAGCAG 
CTGCTGTGGG AGGCGTACGA TGAGTACACG GTGGCTGAAT TTGTAGAGCA GCACATCTAT 
CCTACCATCG AGGCGCTGCA GCGCCAATTG GCCAGCGGAG CGACGCTGCT CCAACGCCGC 
ACCTGGCCAC AACGGCCGCT GCCATTGACC CTGAGGATAC AGGAGGATAT GGGCTTGGTC 
ACAGATCGCC AGCCGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001359965.2
CDS	153..2273
Translation	MHSNLWIFIWRRKLSFLLVVSGLVLLGLWTWAILIDTTTTPPLPVPSANAEARERQQQQQNPGYQQVHIIQKLIAQANRVLRADRLKDNAAGEKAPTVQRLGNVRFLRPTQAKKAPLPLSESYLFEQERLKILEQQQRQRNAGMEELNANAEDERMLSSAERQTQYEHDLQRMGVPVVAGIGPDGPRSPNERLVHLDLKGAPPKLSFLKQLLPMLRALGATGLLIEYEDMFPFTGALQPLAAHNAYKEDELRDFLECAALHGLSVMPLLQTFGHLEYVLKLEGFEQLRELPESPQSICPSQPQSMALLEQMLTQVIELHLQCIGKATPSAPTLPIKFTHIHIGCDEVQRMGECQACRQRLRSELFLSHVISLSHFVRRHWPQLGVVIWDDQLRSMSLSELQHSQIGSYVEPMVWVYASDIYRFIQPQLWDTYAKVFPSAWVASAYKGAFGESLLVPPLQHHLENNIRWLAVIAKEGGRFAKGLRGVALTGWQRYDHFAVLCELLPVGMPSLMVSLSTVSKGYFSTNPRDNDLLRVLQCVFQPDSRRSGRPWLELHPNAHHSQLFAVCNYPGHMVFKYALRLYDKLAEVGAYLQQTREQSAWLSDYNVRHNFSSPLRVHELIARTPILMEELRAMAREAQQLLWEAYDEYTVAEFVEQHIYPTIEALQRQLASGATLLQRRTWPQRPLPLTLRIQEDMGLVTDRQPH

Target ORF information:

RefSeq Version	XM_001359928.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA20741), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001359928.3

ORF Insert Sequence:

ATGCACTCGA ATCTGTGGAT ATTTATTTGG CGCCGCAAAC TCTCCTTCCT GCTAGTAGTG 
TCTGGCCTCG TGCTGCTGGG TCTCTGGACA TGGGCCATAC TCATCGATAC GACCACCACG 
CCACCACTGC CGGTTCCCTC AGCGAATGCT GAGGCAAGAG AGCGCCAACA GCAGCAGCAG 
AACCCGGGAT ACCAGCAGGT CCATATTATC CAGAAGCTAA TTGCCCAGGC CAACCGTGTG 
CTCCGCGCCG ATCGACTCAA GGACAACGCC GCCGGAGAGA AGGCGCCAAC TGTCCAGCGT 
CTGGGCAACG TGCGATTCCT GCGTCCCACC CAGGCCAAGA AGGCCCCACT GCCGCTGTCC 
GAGAGCTATC TGTTCGAGCA GGAGCGCCTG AAGATACTCG AACAGCAGCA GCGTCAGCGC 
AACGCCGGAA TGGAGGAGCT GAATGCCAAT GCCGAGGATG AGCGTATGCT CAGCTCTGCC 
GAGCGCCAGA CCCAGTACGA GCACGATCTC CAGCGGATGG GCGTTCCCGT GGTGGCTGGT 
ATCGGACCAG ACGGGCCACG TTCCCCTAAC GAGCGTCTCG TCCACTTGGA TCTGAAGGGG 
GCCCCGCCCA AGCTGAGTTT CCTCAAGCAA CTGCTGCCGA TGCTCAGGGC TCTGGGTGCC 
ACGGGGCTAC TGATCGAATA CGAGGATATG TTTCCCTTTA CTGGAGCCCT CCAGCCGCTG 
GCCGCCCACA ATGCCTACAA AGAAGATGAA TTGAGGGACT TTCTGGAGTG CGCCGCCTTG 
CATGGTTTGA GTGTGATGCC TCTGCTTCAG ACCTTCGGGC ATTTGGAGTA TGTCCTGAAA 
CTGGAGGGAT TCGAGCAGCT GCGAGAGCTG CCCGAAAGTC CCCAATCCAT CTGCCCCAGT 
CAGCCGCAGA GCATGGCCCT GCTGGAGCAG ATGCTCACCC AGGTGATCGA GCTGCATTTA 
CAGTGCATCG GCAAGGCCAC CCCCTCAGCC CCGACACTGC CCATCAAGTT CACGCACATC 
CACATCGGAT GCGATGAGGT GCAGCGCATG GGGGAGTGCC AGGCCTGCCG CCAGAGACTC 
CGGAGCGAGC TGTTCCTGTC GCACGTGATC AGTCTCTCGC ACTTTGTGCG TCGCCACTGG 
CCCCAACTGG GCGTGGTCAT CTGGGATGAT CAGCTGCGAT CGATGTCACT CAGCGAGCTG 
CAACACTCGC AGATCGGCAG CTATGTGGAG CCCATGGTGT GGGTGTATGC CAGCGATATC 
TATCGGTTCA TCCAGCCGCA GCTGTGGGAC ACCTATGCCA AGGTCTTTCC CAGTGCTTGG 
GTGGCCTCGG CCTACAAGGG AGCCTTCGGC GAGAGCCTGC TGGTGCCGCC GCTGCAGCAT 
CATCTGGAAA ATAACATACG CTGGCTGGCG GTCATCGCCA AGGAGGGCGG ACGCTTTGCC 
AAAGGACTGC GCGGTGTGGC TCTAACCGGC TGGCAGCGAT ACGATCACTT TGCCGTCCTG 
TGCGAACTGC TGCCAGTGGG AATGCCCAGC CTGATGGTGT CGCTGTCGAC TGTTTCCAAG 
GGTTATTTCA GCACTAATCC AAGGGACAAC GATCTGCTGC GCGTGCTGCA GTGCGTCTTT 
CAGCCGGACA GCCGGCGCTC GGGTCGCCCC TGGCTGGAGC TTCATCCGAA TGCCCATCAT 
AGCCAACTGT TTGCTGTCTG CAACTATCCC GGCCACATGG TCTTCAAGTA TGCCCTTCGC 
CTGTACGACA AACTGGCCGA AGTGGGAGCC TATCTACAGC AGACGCGCGA GCAGAGCGCC 
TGGCTCTCCG ACTACAATGT GAGGCACAAC TTCAGCTCCC CGTTGAGAGT TCACGAGCTG 
ATTGCCCGAA CGCCAATACT AATGGAGGAG CTGCGCGCAA TGGCTCGCGA GGCACAGCAG 
CTGCTGTGGG AGGCGTACGA TGAGTACACG GTGGCTGAAT TTGTAGAGCA GCACATCTAT 
CCTACCATCG AGGCGCTGCA GCGCCAATTG GCCAGCGGAG CGACGCTGCT CCAACGCCGC 
ACCTGGCCAC AACGGCCGCT GCCATTGACC CTGAGGATAC AGGAGGATAT GGGCTTGGTC 
ACAGATCGCC AGCCGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA20741 ( XM_015182852.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015038338.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20741), mRNA.

Price & Availability↑

CloneID	ODe20957
Clone ID Related Accession (Same CDS sequence)	XM_015182852.1
Accession Version	XM_015182852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGCGA ACTTTTCGCA CAGACTTTTG TGCGTCGCTG TTCGTCGCCA GATGCACTCG 
AATCTGTGGA TATTTATTTG GCGCCGCAAA CTCTCCTTCC TGCTAGTAGT GTCTGGCCTC 
GTGCTGCTGG GTCTCTGGAC ATGGGCCATA CTCATCGATA CGACCACCAC GCCACCACTG 
CCGGTTCCCT CAGCGAATGC TGAGGCAAGA GAGCGCCAAC AGCAGCAGCA GAACCCGGGA 
TACCAGCAGG TCCATATTAT CCAGAAGCTA ATTGCCCAGG CCAACCGTGT GCTCCGCGCC 
GATCGACTCA AGGACAACGC CGCCGGAGAG AAGGCGCCAA CTGTCCAGCG TCTGGGCAAC 
GTGCGATTCC TGCGTCCCAC CCAGGCCAAG AAGGCCCCAC TGCCGCTGTC CGAGAGCTAT 
CTGTTCGAGC AGGAGCGCCT GAAGATACTC GAACAGCAGC AGCGTCAGCG CAACGCCGGA 
ATGGAGGAGC TGAATGCCAA TGCCGAGGAT GAGCGTATGC TCAGCTCTGC CGAGCGCCAG 
ACCCAGTACG AGCACGATCT CCAGCGGATG GGCGTTCCCG TGGTGGCTGG TATCGGACCA 
GACGGGCCAC GTTCCCCTAA CGAGCGTCTC GTCCACTTGG ATCTGAAGGG GGCCCCGCCC 
AAGCTGAGTT TCCTCAAGCA ACTGCTGCCG ATGCTCAGGG CTCTGGGTGC CACGGGGCTA 
CTGATCGAAT ACGAGGATAT GTTTCCCTTT ACTGGAGCCC TCCAGCCGCT GGCCGCCCAC 
AATGCCTACA AAGAAGATGA ATTGAGGGAC TTTCTGGAGT GCGCCGCCTT GCATGGTTTG 
AGTGTGATGC CTCTGCTTCA GACCTTCGGG CATTTGGAGT ATGTCCTGAA ACTGGAGGGA 
TTCGAGCAGC TGCGAGAGCT GCCCGAAAGT CCCCAATCCA TCTGCCCCAG TCAGCCGCAG 
AGCATGGCCC TGCTGGAGCA GATGCTCACC CAGGTGATCG AGCTGCATTT ACAGTGCATC 
GGCAAGGCCA CCCCCTCAGC CCCGACACTG CCCATCAAGT TCACGCACAT CCACATCGGA 
TGCGATGAGG TGCAGCGCAT GGGGGAGTGC CAGGCCTGCC GCCAGAGACT CCGGAGCGAG 
CTGTTCCTGT CGCACGTGAT CAGTCTCTCG CACTTTGTGC GTCGCCACTG GCCCCAACTG 
GGCGTGGTCA TCTGGGATGA TCAGCTGCGA TCGATGTCAC TCAGCGAGCT GCAACACTCG 
CAGATCGGCA GCTATGTGGA GCCCATGGTG TGGGTGTATG CCAGCGATAT CTATCGGTTC 
ATCCAGCCGC AGCTGTGGGA CACCTATGCC AAGGTCTTTC CCAGTGCTTG GGTGGCCTCG 
GCCTACAAGG GAGCCTTCGG CGAGAGCCTG CTGGTGCCGC CGCTGCAGCA TCATCTGGAA 
AATAACATAC GCTGGCTGGC GGTCATCGCC AAGGAGGGCG GACGCTTTGC CAAAGGACTG 
CGCGGTGTGG CTCTAACCGG CTGGCAGCGA TACGATCACT TTGCCGTCCT GTGCGAACTG 
CTGCCAGTGG GAATGCCCAG CCTGATGGTG TCGCTGTCGA CTGTTTCCAA GGGTTATTTC 
AGCACTAATC CAAGGGACAA CGATCTGCTG CGCGTGCTGC AGTGCGTCTT TCAGCCGGAC 
AGCCGGCGCT CGGGTCGCCC CTGGCTGGAG CTTCATCCGA ATGCCCATCA TAGCCAACTG 
TTTGCTGTCT GCAACTATCC CGGCCACATG GTCTTCAAGT ATGCCCTTCG CCTGTACGAC 
AAACTGGCCG AAGTGGGAGC CTATCTACAG CAGACGCGCG AGCAGAGCGC CTGGCTCTCC 
GACTACAATG TGAGGCACAA CTTCAGCTCC CCGTTGAGAG TTCACGAGCT GATTGCCCGA 
ACGCCAATAC TAATGGAGGA GCTGCGCGCA ATGGCTCGCG AGGCACAGCA GCTGCTGTGG 
GAGGCGTACG ATGAGTACAC GGTGGCTGAA TTTGTAGAGC AGCACATCTA TCCTACCATC 
GAGGCGCTGC AGCGCCAATT GGCCAGCGGA GCGACGCTGC TCCAACGCCG CACCTGGCCA 
CAACGGCCGC TGCCATTGAC CCTGAGGATA CAGGAGGATA TGGGCTTGGT CACAGATCGC 
CAGCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015038338.1
CDS	82..2253
Translation	MYANFSHRLLCVAVRRQMHSNLWIFIWRRKLSFLLVVSGLVLLGLWTWAILIDTTTTPPLPVPSANAEARERQQQQQNPGYQQVHIIQKLIAQANRVLRADRLKDNAAGEKAPTVQRLGNVRFLRPTQAKKAPLPLSESYLFEQERLKILEQQQRQRNAGMEELNANAEDERMLSSAERQTQYEHDLQRMGVPVVAGIGPDGPRSPNERLVHLDLKGAPPKLSFLKQLLPMLRALGATGLLIEYEDMFPFTGALQPLAAHNAYKEDELRDFLECAALHGLSVMPLLQTFGHLEYVLKLEGFEQLRELPESPQSICPSQPQSMALLEQMLTQVIELHLQCIGKATPSAPTLPIKFTHIHIGCDEVQRMGECQACRQRLRSELFLSHVISLSHFVRRHWPQLGVVIWDDQLRSMSLSELQHSQIGSYVEPMVWVYASDIYRFIQPQLWDTYAKVFPSAWVASAYKGAFGESLLVPPLQHHLENNIRWLAVIAKEGGRFAKGLRGVALTGWQRYDHFAVLCELLPVGMPSLMVSLSTVSKGYFSTNPRDNDLLRVLQCVFQPDSRRSGRPWLELHPNAHHSQLFAVCNYPGHMVFKYALRLYDKLAEVGAYLQQTREQSAWLSDYNVRHNFSSPLRVHELIARTPILMEELRAMAREAQQLLWEAYDEYTVAEFVEQHIYPTIEALQRQLASGATLLQRRTWPQRPLPLTLRIQEDMGLVTDRQPH

Target ORF information:

RefSeq Version	XM_015182852.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA20741), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015182852.1

ORF Insert Sequence:

ATGTACGCGA ACTTTTCGCA CAGACTTTTG TGCGTCGCTG TTCGTCGCCA GATGCACTCG 
AATCTGTGGA TATTTATTTG GCGCCGCAAA CTCTCCTTCC TGCTAGTAGT GTCTGGCCTC 
GTGCTGCTGG GTCTCTGGAC ATGGGCCATA CTCATCGATA CGACCACCAC GCCACCACTG 
CCGGTTCCCT CAGCGAATGC TGAGGCAAGA GAGCGCCAAC AGCAGCAGCA GAACCCGGGA 
TACCAGCAGG TCCATATTAT CCAGAAGCTA ATTGCCCAGG CCAACCGTGT GCTCCGCGCC 
GATCGACTCA AGGACAACGC CGCCGGAGAG AAGGCGCCAA CTGTCCAGCG TCTGGGCAAC 
GTGCGATTCC TGCGTCCCAC CCAGGCCAAG AAGGCCCCAC TGCCGCTGTC CGAGAGCTAT 
CTGTTCGAGC AGGAGCGCCT GAAGATACTC GAACAGCAGC AGCGTCAGCG CAACGCCGGA 
ATGGAGGAGC TGAATGCCAA TGCCGAGGAT GAGCGTATGC TCAGCTCTGC CGAGCGCCAG 
ACCCAGTACG AGCACGATCT CCAGCGGATG GGCGTTCCCG TGGTGGCTGG TATCGGACCA 
GACGGGCCAC GTTCCCCTAA CGAGCGTCTC GTCCACTTGG ATCTGAAGGG GGCCCCGCCC 
AAGCTGAGTT TCCTCAAGCA ACTGCTGCCG ATGCTCAGGG CTCTGGGTGC CACGGGGCTA 
CTGATCGAAT ACGAGGATAT GTTTCCCTTT ACTGGAGCCC TCCAGCCGCT GGCCGCCCAC 
AATGCCTACA AAGAAGATGA ATTGAGGGAC TTTCTGGAGT GCGCCGCCTT GCATGGTTTG 
AGTGTGATGC CTCTGCTTCA GACCTTCGGG CATTTGGAGT ATGTCCTGAA ACTGGAGGGA 
TTCGAGCAGC TGCGAGAGCT GCCCGAAAGT CCCCAATCCA TCTGCCCCAG TCAGCCGCAG 
AGCATGGCCC TGCTGGAGCA GATGCTCACC CAGGTGATCG AGCTGCATTT ACAGTGCATC 
GGCAAGGCCA CCCCCTCAGC CCCGACACTG CCCATCAAGT TCACGCACAT CCACATCGGA 
TGCGATGAGG TGCAGCGCAT GGGGGAGTGC CAGGCCTGCC GCCAGAGACT CCGGAGCGAG 
CTGTTCCTGT CGCACGTGAT CAGTCTCTCG CACTTTGTGC GTCGCCACTG GCCCCAACTG 
GGCGTGGTCA TCTGGGATGA TCAGCTGCGA TCGATGTCAC TCAGCGAGCT GCAACACTCG 
CAGATCGGCA GCTATGTGGA GCCCATGGTG TGGGTGTATG CCAGCGATAT CTATCGGTTC 
ATCCAGCCGC AGCTGTGGGA CACCTATGCC AAGGTCTTTC CCAGTGCTTG GGTGGCCTCG 
GCCTACAAGG GAGCCTTCGG CGAGAGCCTG CTGGTGCCGC CGCTGCAGCA TCATCTGGAA 
AATAACATAC GCTGGCTGGC GGTCATCGCC AAGGAGGGCG GACGCTTTGC CAAAGGACTG 
CGCGGTGTGG CTCTAACCGG CTGGCAGCGA TACGATCACT TTGCCGTCCT GTGCGAACTG 
CTGCCAGTGG GAATGCCCAG CCTGATGGTG TCGCTGTCGA CTGTTTCCAA GGGTTATTTC 
AGCACTAATC CAAGGGACAA CGATCTGCTG CGCGTGCTGC AGTGCGTCTT TCAGCCGGAC 
AGCCGGCGCT CGGGTCGCCC CTGGCTGGAG CTTCATCCGA ATGCCCATCA TAGCCAACTG 
TTTGCTGTCT GCAACTATCC CGGCCACATG GTCTTCAAGT ATGCCCTTCG CCTGTACGAC 
AAACTGGCCG AAGTGGGAGC CTATCTACAG CAGACGCGCG AGCAGAGCGC CTGGCTCTCC 
GACTACAATG TGAGGCACAA CTTCAGCTCC CCGTTGAGAG TTCACGAGCT GATTGCCCGA 
ACGCCAATAC TAATGGAGGA GCTGCGCGCA ATGGCTCGCG AGGCACAGCA GCTGCTGTGG 
GAGGCGTACG ATGAGTACAC GGTGGCTGAA TTTGTAGAGC AGCACATCTA TCCTACCATC 
GAGGCGCTGC AGCGCCAATT GGCCAGCGGA GCGACGCTGC TCCAACGCCG CACCTGGCCA 
CAACGGCCGC TGCCATTGAC CCTGAGGATA CAGGAGGATA TGGGCTTGGT CACAGATCGC 
CAGCCGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA20741 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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