Spn28F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spn28F
Entrez Gene ID	49807
Full Name	Serpin 28F
Synonyms	CG8137,Dmel\CG8137,Sp2,Spn2,sp2,spn2
General protein information	Preferred Names Serpin 28F Names CG8137 gene product from transcript CG8137-RA CG8137-PA Spn28F-PA serine protease inhibitor 2 serpin 2 serpin 28F
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28F5-28F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_080218.5	NP_524957.2	serpin 28F

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0610800	NP_001050652.1
Arabidopsis thaliana (thale cress)	AT1G62160	NP_176407.2
Macaca mulatta (Rhesus monkey)	SERPINB3	XP_001091267.1
Bos taurus (cattle)	SERPINB4	NP_001095792.2
Bos taurus (cattle)	LOC786348	XP_003587825.1
Drosophila melanogaster (fruit fly)	Spn42Dd	NP_001163067.1
Arabidopsis thaliana (thale cress)	AT3G45220	NP_190108.1
Arabidopsis thaliana (thale cress)	AT2G25240	NP_180096.3
Oryza sativa (rice)	Os03g0610650	NP_001173533.1
Homo sapiens (human)	SERPINB4	NP_002965.1
Pan troglodytes (chimpanzee)	SERPINB4	XP_512165.2
Drosophila melanogaster (fruit fly)	Spn42De	NP_610246.3
Drosophila melanogaster (fruit fly)	Spn28B	NP_001260209.1
Drosophila melanogaster (fruit fly)	Spn28F	NP_524957.2
Caenorhabditis elegans (roundworm)	srp-3	NP_503528.2
Caenorhabditis elegans (roundworm)	srp-8	NP_505108.1
Arabidopsis thaliana (thale cress)	AT1G64010	NP_176584.1
Xenopus tropicalis (tropical clawed frog)	LOC100490314	XP_002936465.1
Canis lupus familiaris (dog)	SERPINB4	XP_541074.2
Gallus gallus (chicken)	OVAL	NP_990483.1
Drosophila melanogaster (fruit fly)	Spn38F	NP_524956.2
Caenorhabditis elegans (roundworm)	srp-6	NP_504890.1
Caenorhabditis elegans (roundworm)	srp-5	NP_504889.2
Arabidopsis thaliana (thale cress)	SRP2	NP_179060.1
Arabidopsis thaliana (thale cress)	AT2G35580	NP_181101.1
Bos taurus (cattle)	LOC511106	NP_001099089.1
Rattus norvegicus (Norway rat)	Serpinb3a	XP_006249702.1
Gallus gallus (chicken)	OVALX	NP_001263315.1
Drosophila melanogaster (fruit fly)	Spn28Da	NP_001036345.1
Drosophila melanogaster (fruit fly)	Spn28Db	NP_787995.2
Caenorhabditis elegans (roundworm)	srp-7	NP_001023823.1
Caenorhabditis elegans (roundworm)	srp-1	NP_503315.1
Arabidopsis thaliana (thale cress)	AT2G26390	NP_180207.1
Arabidopsis thaliana (thale cress)	SRP3	NP_176586.1
Oryza sativa (rice)	Os03g0610700	NP_001050651.1
Macaca mulatta (Rhesus monkey)	SERPINB4	XP_001090785.1
Bos taurus (cattle)	LOC786410	NP_001193642.1
Mus musculus (house mouse)	Serpinb3a	NP_033152.3
Gallus gallus (chicken)	OVALY	NP_001026172.1
Caenorhabditis elegans (roundworm)	srp-2	NP_503318.1
Arabidopsis thaliana (thale cress)	AT1G47710	NP_175202.1
Arabidopsis thaliana (thale cress)	AT1G62170	NP_176408.1
Bos taurus (cattle)	LOC100337240	XP_002697900.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spn28F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spn28F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15286	NM_080218.5 Latest version!	Drosophila melanogaster serpin 28F (Spn28F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spn28F ( NM_080218.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524957.2 Drosophila melanogaster serpin 28F (Spn28F), mRNA.

Price & Availability↑

CloneID	OFa15286
Clone ID Related Accession (Same CDS sequence)	NM_080218.5
Accession Version	NM_080218.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	serpin 28F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_080218.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCATT TGTGTCTGCT TCTTTTGGCC ACGTCGGTAT CTTGTCGCTT TGCCGACGAT 
TTCTACCAAC TTTTGGCCAA GGAAAATGCG GCCAATAATC TCATTTCCTC TCCCCTTTCC 
GTGGAAATCG CACTGAGCAT GGCCTACATG GGAGCCAGGG CAAAGACAGC CCAAGAAATG 
AGGAATGTTT TGAAACTGCC CGATGACAAA AAGGAAGTGG CAGCCAAATA CAAGGACCTT 
CTGAGTAAAC TCGAAGGACG CGAGAAGGTA GCCACTCTCA GTTTGGCTAA TCGCATATAC 
GTGAACAAAA AGTTCCAGCT AGTACCGAGT TACAATCAGA TGGTCAAGGA TTCATTTATG 
GCTGAAGCGG AGGCAATCGA CATTGTCGAT CCCAATAAGG CTTCTTCAAT TGTCAATAAT 
TGGGTAGATA ATCAGACGCG TGGTAAAATC AAAGACTTAG TTTCATCCAA TGACATGTCG 
AAAATGGAAT TGATCGTCTT AAATGCCATA TACTTTAAAG GCCAGTGGGA GTACAAATTT 
AATCCCAAGT TAACGAAGAA GAGGAATTTC CGTGTTTCGG ATCAAAAGAG TGTTCCTGTC 
GAGATGATGT CACTATTTCA ATCATTTAGG GCCGCACATG ATAGTGAACT GGGTGCCAAA 
ATTATCGAGC TGCCCTACAG AAATTCAAGC CTGTCCATGC TAATTTTTCT ACCGGACCAG 
GTCGATGGAC TATCAGAGCT GGAGAAAAAG ATTGTAGGAT TCAAACCGAA ACTTTCCAAA 
ATGGATGTAA CTTTAAGGCT TCCCAAGTTT AAAATCGAAT TCTTCGCTCA ACTAAACAAA 
GTTCTTGTGG CGATGGGCAT ACAAGATGCG TTCGAAAAAT CTGCTGACTT CAAAGATCTG 
GTCGAAAACA GTAACGTTCA TGTGAAAAAA GTCATTCATA AGGCGTTCAT CGAGGTTAAT 
GAAGAAGGAG CTGAGGCAGC GGCTGCAACA GCTTTACTTT TCGTGCGCTA TAGTATGCCT 
ATGCCGTCAT CTCAAATGGT TTTTAATGCC GATCATCCTT TTGCCTATGT CATTCGCGAT 
AGGGAAACCA TTTATTTCCA GGGCCACTTT GTGAAACCGA ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524957.2
CDS	35..1162
Translation	MKHLCLLLLATSVSCRFADDFYQLLAKENAANNLISSPLSVEIALSMAYMGARAKTAQEMRNVLKLPDDKKEVAAKYKDLLSKLEGREKVATLSLANRIYVNKKFQLVPSYNQMVKDSFMAEAEAIDIVDPNKASSIVNNWVDNQTRGKIKDLVSSNDMSKMELIVLNAIYFKGQWEYKFNPKLTKKRNFRVSDQKSVPVEMMSLFQSFRAAHDSELGAKIIELPYRNSSLSMLIFLPDQVDGLSELEKKIVGFKPKLSKMDVTLRLPKFKIEFFAQLNKVLVAMGIQDAFEKSADFKDLVENSNVHVKKVIHKAFIEVNEEGAEAAAATALLFVRYSMPMPSSQMVFNADHPFAYVIRDRETIYFQGHFVKPNE

Target ORF information:

RefSeq Version	NM_080218.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serpin 28F (Spn28F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080218.5

ORF Insert Sequence:

ATGAAGCATT TGTGTCTGCT TCTTTTGGCC ACGTCGGTAT CTTGTCGCTT TGCCGACGAT 
TTCTACCAAC TTTTGGCCAA GGAAAATGCG GCCAATAATC TCATTTCCTC TCCCCTTTCC 
GTGGAAATCG CACTGAGCAT GGCCTACATG GGAGCCAGGG CAAAGACAGC CCAAGAAATG 
AGGAATGTTT TGAAACTGCC CGATGACAAA AAGGAAGTGG CAGCCAAATA CAAGGACCTT 
CTGAGTAAAC TCGAAGGACG CGAGAAGGTA GCCACTCTCA GTTTGGCTAA TCGCATATAC 
GTGAACAAAA AGTTCCAGCT AGTACCGAGT TACAATCAGA TGGTCAAGGA TTCATTTATG 
GCTGAAGCGG AGGCAATCGA CATTGTCGAT CCCAATAAGG CTTCTTCAAT TGTCAATAAT 
TGGGTAGATA ATCAGACGCG TGGTAAAATC AAAGACTTAG TTTCATCCAA TGACATGTCG 
AAAATGGAAT TGATCGTCTT AAATGCCATA TACTTTAAAG GCCAGTGGGA GTACAAATTT 
AATCCCAAGT TAACGAAGAA GAGGAATTTC CGTGTTTCGG ATCAAAAGAG TGTTCCTGTC 
GAGATGATGT CACTATTTCA ATCATTTAGG GCCGCACATG ATAGTGAACT GGGTGCCAAA 
ATTATCGAGC TGCCCTACAG AAATTCAAGC CTGTCCATGC TAATTTTTCT ACCGGACCAG 
GTCGATGGAC TATCAGAGCT GGAGAAAAAG ATTGTAGGAT TCAAACCGAA ACTTTCCAAA 
ATGGATGTAA CTTTAAGGCT TCCCAAGTTT AAAATCGAAT TCTTCGCTCA ACTAAACAAA 
GTTCTTGTGG CGATGGGCAT ACAAGATGCG TTCGAAAAAT CTGCTGACTT CAAAGATCTG 
GTCGAAAACA GTAACGTTCA TGTGAAAAAA GTCATTCATA AGGCGTTCAT CGAGGTTAAT 
GAAGAAGGAG CTGAGGCAGC GGCTGCAACA GCTTTACTTT TCGTGCGCTA TAGTATGCCT 
ATGCCGTCAT CTCAAATGGT TTTTAATGCC GATCATCCTT TTGCCTATGT CATTCGCGAT 
AGGGAAACCA TTTATTTCCA GGGCCACTTT GTGAAACCGA ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spn28F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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