Act87E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Act87E
Entrez Gene ID	48632
Full Name	Actin 87E
Synonyms	Act87e,Actin,Actin87E,ArpC,CG18290,Dmel\CG18290,E,act 87E,act87E,actin,mus87E
General protein information	Preferred Names Actin 87E Names CG18290 gene product from transcript CG18290-RC Act87E-PA Act87E-PB Act87E-PC CG18290-PA CG18290-PB CG18290-PC actin actin 87E actin87E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87E11-87E11

mRNA and Protein(s)

mRNA	Protein	Name
NM_057743.5	NP_477091.1	actin 87E, isoform A
NM_169525.2	NP_731812.1	actin 87E, isoform B
NM_001300385.1	NP_001287314.1	actin 87E, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011516	XP_315269.4
Homo sapiens (human)	ACTB	NP_001092.1
Gallus gallus (chicken)	ACTB	NP_990849.1
Oryza sativa (rice)	Os05g0438800	NP_001055661.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005095	XP_313971.4
Pan troglodytes (chimpanzee)	ACTB	NP_001009945.1
Rattus norvegicus (Norway rat)	Actb	NP_112406.1
Macaca mulatta (Rhesus monkey)	LOC100428865	XP_002802770.1
Canis lupus familiaris (dog)	ACTB	NP_001182774.1
Drosophila melanogaster (fruit fly)	Act57B	NP_523800.1
Xenopus tropicalis (tropical clawed frog)	actb	NP_998884.1
Macaca mulatta (Rhesus monkey)	ACTB	NP_001028256.1
Mus musculus (house mouse)	Actb	NP_031419.1
Bos taurus (cattle)	ACTB	NP_776404.2
Drosophila melanogaster (fruit fly)	Act87E	NP_731812.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Act87E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Act87E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08906	NM_057743.5 Latest version!	Drosophila melanogaster actin 87E, transcript variant A (Act87E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa08906	NM_169525.2 Latest version!	Drosophila melanogaster actin 87E, transcript variant B (Act87E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa08906	NM_001300385.1 Latest version!	Drosophila melanogaster actin 87E, transcript variant C (Act87E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Act87E ( NM_057743.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477091.1 Drosophila melanogaster actin 87E, transcript variant A (Act87E), mRNA.

Price & Availability↑

CloneID	OFa08906
Clone ID Related Accession (Same CDS sequence)	NM_057743.5 , NM_169525.2 , NM_001300385.1
Accession Version	NM_057743.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	actin 87E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057743.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGACG ATGAGGTTGC CGCATTGGTC GTGGACAATG GTTCCGGAAT GTGCAAGGCA 
GGATTCGCCG GCGATGATGC GCCCCGCGCC GTCTTCCCCT CGATTGTGGG TCGTCCCCGT 
CATCAGGGCG TAATGGTGGG CATGGGACAG AAGGACTCCT ATGTTGGTGA TGAGGCCCAG 
AGCAAGCGTG GTATCCTCAC CCTGAAATAC CCCATCGAGC ACGGCATCAT CACCAACTGG 
GACGATATGG AGAAGATCTG GCACCACACT TTCTATAACG AGCTGCGCGT CGCCCCCGAG 
GAACACCCCG TCCTGCTGAC CGAGGCCCCC CTGAACCCCA AGGCCAATCG CGAGAAGATG 
ACCCAGATCA TGTTCGAGAC CTTCAACGCA CCCGCCATGT ATGTGGCCAT CCAGGCTGTG 
CTCTCGCTGT ACGCCTCCGG TCGTACCACC GGTATTGTCC TCGACTCCGG TGACGGTGTC 
TCCCACACCG TGCCCATCTA CGAGGGTTAC GCCCTGCCCC ACGCCATCCT GCGTCTGGAT 
CTGGCTGGTC GCGATTTGAC CGACTACCTG ATGAAGATCC TGACCGAGCG CGGTTACTCA 
TTCACCACCA CCGCTGAGCG TGAAATCGTT CGCGACATCA AGGAGAAGCT GTGCTATGTT 
GCCCTGGACT TTGAGCAGGA GATGGCCACC GCCGCCGCCT CCACATCCCT GGAGAAGTCA 
TACGAGCTTC CCGACGGACA GGTGATCACC ATCGGCAACG AACGTTTCCG CTGCCCAGAG 
TCGCTGTTCC AGCCCTCTTT CCTGGGAATG GAATCGTGCG GCATCCACGA GACCGTGTAC 
AACTCGATCA TGAAGTGCGA TGTGGACATC CGTAAGGATC TGTATGCTAA CATCGTCATG 
TCGGGTGGTA CCACCATGTA CCCTGGTATT GCCGATCGTA TGCAGAAGGA GATCACCGCC 
CTGGCGCCGT CCACCATCAA GATCAAGATC ATTGCCCCAC CGGAGCGCAA GTACTCCGTC 
TGGATCGGTG GCTCCATCCT GGCCTCCCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG 
CAGGAGTACG ACGAGTCCGG CCCAGGAATC GTCCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477091.1
CDS	82..1212
Translation	MCDDEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPESLFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANIVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVHRKCF

Target ORF information:

RefSeq Version	NM_057743.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster actin 87E, transcript variant A (Act87E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057743.5

ORF Insert Sequence:

ATGTGTGACG ATGAGGTTGC CGCATTGGTC GTGGACAATG GTTCCGGAAT GTGCAAGGCA 
GGATTCGCCG GCGATGATGC GCCCCGCGCC GTCTTCCCCT CGATTGTGGG TCGTCCCCGT 
CATCAGGGCG TAATGGTGGG CATGGGACAG AAGGACTCCT ATGTTGGTGA TGAGGCCCAG 
AGCAAGCGTG GTATCCTCAC CCTGAAATAC CCCATCGAGC ACGGCATCAT CACCAACTGG 
GACGATATGG AGAAGATCTG GCACCACACT TTCTATAACG AGCTGCGCGT CGCCCCCGAG 
GAACACCCCG TCCTGCTGAC CGAGGCCCCC CTGAACCCCA AGGCCAATCG CGAGAAGATG 
ACCCAGATCA TGTTCGAGAC CTTCAACGCA CCCGCCATGT ATGTGGCCAT CCAGGCTGTG 
CTCTCGCTGT ACGCCTCCGG TCGTACCACC GGTATTGTCC TCGACTCCGG TGACGGTGTC 
TCCCACACCG TGCCCATCTA CGAGGGTTAC GCCCTGCCCC ACGCCATCCT GCGTCTGGAT 
CTGGCTGGTC GCGATTTGAC CGACTACCTG ATGAAGATCC TGACCGAGCG CGGTTACTCA 
TTCACCACCA CCGCTGAGCG TGAAATCGTT CGCGACATCA AGGAGAAGCT GTGCTATGTT 
GCCCTGGACT TTGAGCAGGA GATGGCCACC GCCGCCGCCT CCACATCCCT GGAGAAGTCA 
TACGAGCTTC CCGACGGACA GGTGATCACC ATCGGCAACG AACGTTTCCG CTGCCCAGAG 
TCGCTGTTCC AGCCCTCTTT CCTGGGAATG GAATCGTGCG GCATCCACGA GACCGTGTAC 
AACTCGATCA TGAAGTGCGA TGTGGACATC CGTAAGGATC TGTATGCTAA CATCGTCATG 
TCGGGTGGTA CCACCATGTA CCCTGGTATT GCCGATCGTA TGCAGAAGGA GATCACCGCC 
CTGGCGCCGT CCACCATCAA GATCAAGATC ATTGCCCCAC CGGAGCGCAA GTACTCCGTC 
TGGATCGGTG GCTCCATCCT GGCCTCCCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG 
CAGGAGTACG ACGAGTCCGG CCCAGGAATC GTCCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Act87E ( NM_169525.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731812.1 Drosophila melanogaster actin 87E, transcript variant B (Act87E), mRNA.

Price & Availability↑

CloneID	OFa08906
Clone ID Related Accession (Same CDS sequence)	NM_057743.5 , NM_169525.2 , NM_001300385.1
Accession Version	NM_169525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	actin 87E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_169525.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGACG ATGAGGTTGC CGCATTGGTC GTGGACAATG GTTCCGGAAT GTGCAAGGCA 
GGATTCGCCG GCGATGATGC GCCCCGCGCC GTCTTCCCCT CGATTGTGGG TCGTCCCCGT 
CATCAGGGCG TAATGGTGGG CATGGGACAG AAGGACTCCT ATGTTGGTGA TGAGGCCCAG 
AGCAAGCGTG GTATCCTCAC CCTGAAATAC CCCATCGAGC ACGGCATCAT CACCAACTGG 
GACGATATGG AGAAGATCTG GCACCACACT TTCTATAACG AGCTGCGCGT CGCCCCCGAG 
GAACACCCCG TCCTGCTGAC CGAGGCCCCC CTGAACCCCA AGGCCAATCG CGAGAAGATG 
ACCCAGATCA TGTTCGAGAC CTTCAACGCA CCCGCCATGT ATGTGGCCAT CCAGGCTGTG 
CTCTCGCTGT ACGCCTCCGG TCGTACCACC GGTATTGTCC TCGACTCCGG TGACGGTGTC 
TCCCACACCG TGCCCATCTA CGAGGGTTAC GCCCTGCCCC ACGCCATCCT GCGTCTGGAT 
CTGGCTGGTC GCGATTTGAC CGACTACCTG ATGAAGATCC TGACCGAGCG CGGTTACTCA 
TTCACCACCA CCGCTGAGCG TGAAATCGTT CGCGACATCA AGGAGAAGCT GTGCTATGTT 
GCCCTGGACT TTGAGCAGGA GATGGCCACC GCCGCCGCCT CCACATCCCT GGAGAAGTCA 
TACGAGCTTC CCGACGGACA GGTGATCACC ATCGGCAACG AACGTTTCCG CTGCCCAGAG 
TCGCTGTTCC AGCCCTCTTT CCTGGGAATG GAATCGTGCG GCATCCACGA GACCGTGTAC 
AACTCGATCA TGAAGTGCGA TGTGGACATC CGTAAGGATC TGTATGCTAA CATCGTCATG 
TCGGGTGGTA CCACCATGTA CCCTGGTATT GCCGATCGTA TGCAGAAGGA GATCACCGCC 
CTGGCGCCGT CCACCATCAA GATCAAGATC ATTGCCCCAC CGGAGCGCAA GTACTCCGTC 
TGGATCGGTG GCTCCATCCT GGCCTCCCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG 
CAGGAGTACG ACGAGTCCGG CCCAGGAATC GTCCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731812.1
CDS	158..1288
Translation	MCDDEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPESLFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANIVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVHRKCF

Target ORF information:

RefSeq Version	NM_169525.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster actin 87E, transcript variant B (Act87E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169525.2

ORF Insert Sequence:

ATGTGTGACG ATGAGGTTGC CGCATTGGTC GTGGACAATG GTTCCGGAAT GTGCAAGGCA 
GGATTCGCCG GCGATGATGC GCCCCGCGCC GTCTTCCCCT CGATTGTGGG TCGTCCCCGT 
CATCAGGGCG TAATGGTGGG CATGGGACAG AAGGACTCCT ATGTTGGTGA TGAGGCCCAG 
AGCAAGCGTG GTATCCTCAC CCTGAAATAC CCCATCGAGC ACGGCATCAT CACCAACTGG 
GACGATATGG AGAAGATCTG GCACCACACT TTCTATAACG AGCTGCGCGT CGCCCCCGAG 
GAACACCCCG TCCTGCTGAC CGAGGCCCCC CTGAACCCCA AGGCCAATCG CGAGAAGATG 
ACCCAGATCA TGTTCGAGAC CTTCAACGCA CCCGCCATGT ATGTGGCCAT CCAGGCTGTG 
CTCTCGCTGT ACGCCTCCGG TCGTACCACC GGTATTGTCC TCGACTCCGG TGACGGTGTC 
TCCCACACCG TGCCCATCTA CGAGGGTTAC GCCCTGCCCC ACGCCATCCT GCGTCTGGAT 
CTGGCTGGTC GCGATTTGAC CGACTACCTG ATGAAGATCC TGACCGAGCG CGGTTACTCA 
TTCACCACCA CCGCTGAGCG TGAAATCGTT CGCGACATCA AGGAGAAGCT GTGCTATGTT 
GCCCTGGACT TTGAGCAGGA GATGGCCACC GCCGCCGCCT CCACATCCCT GGAGAAGTCA 
TACGAGCTTC CCGACGGACA GGTGATCACC ATCGGCAACG AACGTTTCCG CTGCCCAGAG 
TCGCTGTTCC AGCCCTCTTT CCTGGGAATG GAATCGTGCG GCATCCACGA GACCGTGTAC 
AACTCGATCA TGAAGTGCGA TGTGGACATC CGTAAGGATC TGTATGCTAA CATCGTCATG 
TCGGGTGGTA CCACCATGTA CCCTGGTATT GCCGATCGTA TGCAGAAGGA GATCACCGCC 
CTGGCGCCGT CCACCATCAA GATCAAGATC ATTGCCCCAC CGGAGCGCAA GTACTCCGTC 
TGGATCGGTG GCTCCATCCT GGCCTCCCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG 
CAGGAGTACG ACGAGTCCGG CCCAGGAATC GTCCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Act87E ( NM_001300385.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287314.1 Drosophila melanogaster actin 87E, transcript variant C (Act87E), mRNA.

Price & Availability↑

CloneID	OFa08906
Clone ID Related Accession (Same CDS sequence)	NM_057743.5 , NM_169525.2 , NM_001300385.1
Accession Version	NM_001300385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	actin 87E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGACG ATGAGGTTGC CGCATTGGTC GTGGACAATG GTTCCGGAAT GTGCAAGGCA 
GGATTCGCCG GCGATGATGC GCCCCGCGCC GTCTTCCCCT CGATTGTGGG TCGTCCCCGT 
CATCAGGGCG TAATGGTGGG CATGGGACAG AAGGACTCCT ATGTTGGTGA TGAGGCCCAG 
AGCAAGCGTG GTATCCTCAC CCTGAAATAC CCCATCGAGC ACGGCATCAT CACCAACTGG 
GACGATATGG AGAAGATCTG GCACCACACT TTCTATAACG AGCTGCGCGT CGCCCCCGAG 
GAACACCCCG TCCTGCTGAC CGAGGCCCCC CTGAACCCCA AGGCCAATCG CGAGAAGATG 
ACCCAGATCA TGTTCGAGAC CTTCAACGCA CCCGCCATGT ATGTGGCCAT CCAGGCTGTG 
CTCTCGCTGT ACGCCTCCGG TCGTACCACC GGTATTGTCC TCGACTCCGG TGACGGTGTC 
TCCCACACCG TGCCCATCTA CGAGGGTTAC GCCCTGCCCC ACGCCATCCT GCGTCTGGAT 
CTGGCTGGTC GCGATTTGAC CGACTACCTG ATGAAGATCC TGACCGAGCG CGGTTACTCA 
TTCACCACCA CCGCTGAGCG TGAAATCGTT CGCGACATCA AGGAGAAGCT GTGCTATGTT 
GCCCTGGACT TTGAGCAGGA GATGGCCACC GCCGCCGCCT CCACATCCCT GGAGAAGTCA 
TACGAGCTTC CCGACGGACA GGTGATCACC ATCGGCAACG AACGTTTCCG CTGCCCAGAG 
TCGCTGTTCC AGCCCTCTTT CCTGGGAATG GAATCGTGCG GCATCCACGA GACCGTGTAC 
AACTCGATCA TGAAGTGCGA TGTGGACATC CGTAAGGATC TGTATGCTAA CATCGTCATG 
TCGGGTGGTA CCACCATGTA CCCTGGTATT GCCGATCGTA TGCAGAAGGA GATCACCGCC 
CTGGCGCCGT CCACCATCAA GATCAAGATC ATTGCCCCAC CGGAGCGCAA GTACTCCGTC 
TGGATCGGTG GCTCCATCCT GGCCTCCCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG 
CAGGAGTACG ACGAGTCCGG CCCAGGAATC GTCCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287314.1
CDS	82..1212
Translation	MCDDEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPESLFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANIVMSGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVHRKCF

Target ORF information:

RefSeq Version	NM_001300385.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster actin 87E, transcript variant C (Act87E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300385.1

ORF Insert Sequence:

ATGTGTGACG ATGAGGTTGC CGCATTGGTC GTGGACAATG GTTCCGGAAT GTGCAAGGCA 
GGATTCGCCG GCGATGATGC GCCCCGCGCC GTCTTCCCCT CGATTGTGGG TCGTCCCCGT 
CATCAGGGCG TAATGGTGGG CATGGGACAG AAGGACTCCT ATGTTGGTGA TGAGGCCCAG 
AGCAAGCGTG GTATCCTCAC CCTGAAATAC CCCATCGAGC ACGGCATCAT CACCAACTGG 
GACGATATGG AGAAGATCTG GCACCACACT TTCTATAACG AGCTGCGCGT CGCCCCCGAG 
GAACACCCCG TCCTGCTGAC CGAGGCCCCC CTGAACCCCA AGGCCAATCG CGAGAAGATG 
ACCCAGATCA TGTTCGAGAC CTTCAACGCA CCCGCCATGT ATGTGGCCAT CCAGGCTGTG 
CTCTCGCTGT ACGCCTCCGG TCGTACCACC GGTATTGTCC TCGACTCCGG TGACGGTGTC 
TCCCACACCG TGCCCATCTA CGAGGGTTAC GCCCTGCCCC ACGCCATCCT GCGTCTGGAT 
CTGGCTGGTC GCGATTTGAC CGACTACCTG ATGAAGATCC TGACCGAGCG CGGTTACTCA 
TTCACCACCA CCGCTGAGCG TGAAATCGTT CGCGACATCA AGGAGAAGCT GTGCTATGTT 
GCCCTGGACT TTGAGCAGGA GATGGCCACC GCCGCCGCCT CCACATCCCT GGAGAAGTCA 
TACGAGCTTC CCGACGGACA GGTGATCACC ATCGGCAACG AACGTTTCCG CTGCCCAGAG 
TCGCTGTTCC AGCCCTCTTT CCTGGGAATG GAATCGTGCG GCATCCACGA GACCGTGTAC 
AACTCGATCA TGAAGTGCGA TGTGGACATC CGTAAGGATC TGTATGCTAA CATCGTCATG 
TCGGGTGGTA CCACCATGTA CCCTGGTATT GCCGATCGTA TGCAGAAGGA GATCACCGCC 
CTGGCGCCGT CCACCATCAA GATCAAGATC ATTGCCCCAC CGGAGCGCAA GTACTCCGTC 
TGGATCGGTG GCTCCATCCT GGCCTCCCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG 
CAGGAGTACG ACGAGTCCGG CCCAGGAATC GTCCACCGCA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Act87E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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