bgm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bgm
Entrez Gene ID	44117
Full Name	bubblegum
Synonyms	2a9hom,BG:DS01514.2,BG:DS0514.2,Bgm,CG4501,Dmel\CG4501
General protein information	Preferred Names bubblegum Names CG4501 gene product from transcript CG4501-RC CG4501-PA CG4501-PC bgm-PA bgm-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34F1-34F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298994.1	NP_001285923.1	bubblegum, isoform C
NM_079959.4	NP_524698.1	bubblegum, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Acsbg2	NP_001034203.1
Danio rerio (zebrafish)	acsbg2	NP_001119851.1
Xenopus tropicalis (tropical clawed frog)	acsbg2	NP_001121441.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008596	XP_314697.3
Homo sapiens (human)	ACSBG2	NP_001276106.1
Pan troglodytes (chimpanzee)	ACSBG2	NP_001233536.1
Canis lupus familiaris (dog)	ACSBG2	XP_005633050.1
Drosophila melanogaster (fruit fly)	bgm	NP_524698.1
Macaca mulatta (Rhesus monkey)	ACSBG2	XP_001087092.2
Rattus norvegicus (Norway rat)	Acsbg2	NP_001073565.1
Gallus gallus (chicken)	ACSBG2	NP_001012864.1
Bos taurus (cattle)	ACSBG2	XP_005208962.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bgm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bgm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16418	NM_079959.4 Latest version!	Drosophila melanogaster bubblegum (bgm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OFa16418	NM_001298994.1 Latest version!	Drosophila melanogaster bubblegum (bgm), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bgm ( NM_001298994.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285923.1 Drosophila melanogaster bubblegum (bgm), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16418
Clone ID Related Accession (Same CDS sequence)	NM_001298994.1 , NM_079959.4
Accession Version	NM_001298994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	bubblegum, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCACGA TAGACGCGCT CTACAATCGT CCTGGGCCCA ACCGCCTGCG GCAGGCGGAT 
GCCTATCGCA CCACCAATCG TCAGGATGCC GTCAAGATTC GTATGGCCAA GGATGGAATC 
GGCGCAGAGG AGCCCATCTC CGTGCCCGGC CTGCTGAAGC GTACGGTCAA CAATTATGGC 
GACTATCCTG CGCTGCGCAC CAAGAACGGC AAGAACGGAT ATCACACTGT CACCTACAAA 
CAATATGAGC AGAAGGTGCA CCAGGTGGCC AAGGCGTTCA TTAAGCTCGG TCTGGAGGAG 
CACCATTCGG TGGGTGTGCT GGCCTTCAAT TGCGCCGAAT GGTTCTACTC GGCCATGGGC 
GCCATTCACG CACGAGGCAT CATCGCCGGA ATCTACACCA CCAATTCCGC CGATGCAGTG 
CAGCACGTTC TGGAGAGCTC ACATGCCCAA ATCGTGGTCG TCGACGACGC CAAGCAAATG 
GACAAGATTC ACGCCATTCG CGACAAGCTG CCCAAGCTCA AGGCCGCCAT TCAGATCCAG 
GAGCCCTATT CCCCCTACTT GAAGAAGGAG GATGGCTACT ACAGGTGGTC GGAGATCGAG 
TCGATGAACG TTAGCGACGT GGAGGATCAG TACATGACCC GTTTGGAGAA TGTGGCGATC 
AACGAGTGCT GCTGCCTGGT CTACACCTCC GGAACGGTGG GCATGCCCAA GGGCGTGATG 
CTCTCCCACG ACAACATCAC CTTCGATGTG CGCGGCATCG TCAAGGCCAT GGACCGTGTG 
GTGGTTGGGG CGGAGTCGAT CGTCTCCTAC CTGCCACTTT CGCACGTGGC CGCCCAGACC 
GTGGACATTT ACACCTGCGC CTTTGTGGCG GGCTGCATTT GGTTCGCCGA CAAGGATGCG 
CTGAAGGGAA CGCTGGTGAA GTCGTTGCAG GATGCGCGAC CCACGCGATT CATGGGCGTG 
CCGCGTGTGT ACGAGAAGTT CCAGGAGCGA ATGGTCGCCG TGGCCAGCTC CAGCGGCAGC 
CTGAAGAAGA TGCTCGCCAG CTGGGCCAAG GGCATCACGC TGAAGCACTA CATGGTGAGT 
CAAGGCAAGA GCTCCGGGGG ATTCCGGTAC AAGATTGCCA AGTCGCTCAT CATGTCCAAG 
GTGAAGCAGG CCCTGGGCTT CGATCGCGTC CTTACACTGG CCAGTGCGGC AGCTCCCATG 
TCGCCGGAGA CGAAGAAGTA CTTCCTCAGT CTGGACCTAA AGATTGTCGA TGCCTTCGGC 
ATGTCAGAAA CGGCCGGTTG TCACACCATC TGCCTTCCCG ATTCCGTGGG TCTGAACACA 
ATCGGCAAAA CTTTGCCCGG CTGCGAGTCC AAGTTCATCA ACAAGGATGC CAACGGTCAC 
GGAGAGCTGT GCATCCGAGG ACGTCACGTT TTCATGGGCT ACATCGACAA CAAGGAGAAG 
ACCGAGGAGT CGCTGGATGA CGACTGCTGG CTGCATTCCG GTGATTTGGG ATTTGTGGAT 
GACAAGGGTT ATGTTTCACT GACGGGACGA TCCAAGGAGA TCATCATTAC CGCCGGCGGC 
GAGAACATAC CGCCAGTGCA CATCGAGAAC ACGATCAAGA AGGAGCTGGA TGCCATTTCC 
AATGCCTTTT TGGTGGGCGA GCAGCGCAAA TATCTCACTG TTCTGATCAC CCTAAAGACC 
GAAGTGGACA AGGATTCCGG TGAGCCGCTG GACGAGCTTA GCCACGAGTC CTCCGTGTGG 
GTGAAATCGC TGGGAGTGGA GCACAAGACC GTATCGGATA TCCTGGCCGC AGGTCCCTGC 
CCCAAGGTGT GGAAGTCCAT CGAGGATGCC ATTAAGCGGG CCAACAAGCA GTCCATTTCC 
AATGCCCAAA AGGTTCAGAA GTTCACCATT CTGCCGCACG ACTTCTCCAT TCCCACCGGC 
GAACTTGGAC CCACCCTAAA GGTTAAGCGC AACGTTGTGT CCAAGATGTA TGCCGATGAG 
ATCGAGAAAC TATATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285923.1
CDS	145..2145
Translation	MSTIDALYNRPGPNRLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYRWSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTICLPDSVGLNTIGKTLPGCESKFINKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYADEIEKLYA

Target ORF information:

RefSeq Version	NM_001298994.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bubblegum (bgm), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298994.1

ORF Insert Sequence:

ATGTCCACGA TAGACGCGCT CTACAATCGT CCTGGGCCCA ACCGCCTGCG GCAGGCGGAT 
GCCTATCGCA CCACCAATCG TCAGGATGCC GTCAAGATTC GTATGGCCAA GGATGGAATC 
GGCGCAGAGG AGCCCATCTC CGTGCCCGGC CTGCTGAAGC GTACGGTCAA CAATTATGGC 
GACTATCCTG CGCTGCGCAC CAAGAACGGC AAGAACGGAT ATCACACTGT CACCTACAAA 
CAATATGAGC AGAAGGTGCA CCAGGTGGCC AAGGCGTTCA TTAAGCTCGG TCTGGAGGAG 
CACCATTCGG TGGGTGTGCT GGCCTTCAAT TGCGCCGAAT GGTTCTACTC GGCCATGGGC 
GCCATTCACG CACGAGGCAT CATCGCCGGA ATCTACACCA CCAATTCCGC CGATGCAGTG 
CAGCACGTTC TGGAGAGCTC ACATGCCCAA ATCGTGGTCG TCGACGACGC CAAGCAAATG 
GACAAGATTC ACGCCATTCG CGACAAGCTG CCCAAGCTCA AGGCCGCCAT TCAGATCCAG 
GAGCCCTATT CCCCCTACTT GAAGAAGGAG GATGGCTACT ACAGGTGGTC GGAGATCGAG 
TCGATGAACG TTAGCGACGT GGAGGATCAG TACATGACCC GTTTGGAGAA TGTGGCGATC 
AACGAGTGCT GCTGCCTGGT CTACACCTCC GGAACGGTGG GCATGCCCAA GGGCGTGATG 
CTCTCCCACG ACAACATCAC CTTCGATGTG CGCGGCATCG TCAAGGCCAT GGACCGTGTG 
GTGGTTGGGG CGGAGTCGAT CGTCTCCTAC CTGCCACTTT CGCACGTGGC CGCCCAGACC 
GTGGACATTT ACACCTGCGC CTTTGTGGCG GGCTGCATTT GGTTCGCCGA CAAGGATGCG 
CTGAAGGGAA CGCTGGTGAA GTCGTTGCAG GATGCGCGAC CCACGCGATT CATGGGCGTG 
CCGCGTGTGT ACGAGAAGTT CCAGGAGCGA ATGGTCGCCG TGGCCAGCTC CAGCGGCAGC 
CTGAAGAAGA TGCTCGCCAG CTGGGCCAAG GGCATCACGC TGAAGCACTA CATGGTGAGT 
CAAGGCAAGA GCTCCGGGGG ATTCCGGTAC AAGATTGCCA AGTCGCTCAT CATGTCCAAG 
GTGAAGCAGG CCCTGGGCTT CGATCGCGTC CTTACACTGG CCAGTGCGGC AGCTCCCATG 
TCGCCGGAGA CGAAGAAGTA CTTCCTCAGT CTGGACCTAA AGATTGTCGA TGCCTTCGGC 
ATGTCAGAAA CGGCCGGTTG TCACACCATC TGCCTTCCCG ATTCCGTGGG TCTGAACACA 
ATCGGCAAAA CTTTGCCCGG CTGCGAGTCC AAGTTCATCA ACAAGGATGC CAACGGTCAC 
GGAGAGCTGT GCATCCGAGG ACGTCACGTT TTCATGGGCT ACATCGACAA CAAGGAGAAG 
ACCGAGGAGT CGCTGGATGA CGACTGCTGG CTGCATTCCG GTGATTTGGG ATTTGTGGAT 
GACAAGGGTT ATGTTTCACT GACGGGACGA TCCAAGGAGA TCATCATTAC CGCCGGCGGC 
GAGAACATAC CGCCAGTGCA CATCGAGAAC ACGATCAAGA AGGAGCTGGA TGCCATTTCC 
AATGCCTTTT TGGTGGGCGA GCAGCGCAAA TATCTCACTG TTCTGATCAC CCTAAAGACC 
GAAGTGGACA AGGATTCCGG TGAGCCGCTG GACGAGCTTA GCCACGAGTC CTCCGTGTGG 
GTGAAATCGC TGGGAGTGGA GCACAAGACC GTATCGGATA TCCTGGCCGC AGGTCCCTGC 
CCCAAGGTGT GGAAGTCCAT CGAGGATGCC ATTAAGCGGG CCAACAAGCA GTCCATTTCC 
AATGCCCAAA AGGTTCAGAA GTTCACCATT CTGCCGCACG ACTTCTCCAT TCCCACCGGC 
GAACTTGGAC CCACCCTAAA GGTTAAGCGC AACGTTGTGT CCAAGATGTA TGCCGATGAG 
ATCGAGAAAC TATATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bgm ( NM_079959.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524698.1 Drosophila melanogaster bubblegum (bgm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16418
Clone ID Related Accession (Same CDS sequence)	NM_001298994.1 , NM_079959.4
Accession Version	NM_079959.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	bubblegum, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_079959.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCACGA TAGACGCGCT CTACAATCGT CCTGGGCCCA ACCGCCTGCG GCAGGCGGAT 
GCCTATCGCA CCACCAATCG TCAGGATGCC GTCAAGATTC GTATGGCCAA GGATGGAATC 
GGCGCAGAGG AGCCCATCTC CGTGCCCGGC CTGCTGAAGC GTACGGTCAA CAATTATGGC 
GACTATCCTG CGCTGCGCAC CAAGAACGGC AAGAACGGAT ATCACACTGT CACCTACAAA 
CAATATGAGC AGAAGGTGCA CCAGGTGGCC AAGGCGTTCA TTAAGCTCGG TCTGGAGGAG 
CACCATTCGG TGGGTGTGCT GGCCTTCAAT TGCGCCGAAT GGTTCTACTC GGCCATGGGC 
GCCATTCACG CACGAGGCAT CATCGCCGGA ATCTACACCA CCAATTCCGC CGATGCAGTG 
CAGCACGTTC TGGAGAGCTC ACATGCCCAA ATCGTGGTCG TCGACGACGC CAAGCAAATG 
GACAAGATTC ACGCCATTCG CGACAAGCTG CCCAAGCTCA AGGCCGCCAT TCAGATCCAG 
GAGCCCTATT CCCCCTACTT GAAGAAGGAG GATGGCTACT ACAGGTGGTC GGAGATCGAG 
TCGATGAACG TTAGCGACGT GGAGGATCAG TACATGACCC GTTTGGAGAA TGTGGCGATC 
AACGAGTGCT GCTGCCTGGT CTACACCTCC GGAACGGTGG GCATGCCCAA GGGCGTGATG 
CTCTCCCACG ACAACATCAC CTTCGATGTG CGCGGCATCG TCAAGGCCAT GGACCGTGTG 
GTGGTTGGGG CGGAGTCGAT CGTCTCCTAC CTGCCACTTT CGCACGTGGC CGCCCAGACC 
GTGGACATTT ACACCTGCGC CTTTGTGGCG GGCTGCATTT GGTTCGCCGA CAAGGATGCG 
CTGAAGGGAA CGCTGGTGAA GTCGTTGCAG GATGCGCGAC CCACGCGATT CATGGGCGTG 
CCGCGTGTGT ACGAGAAGTT CCAGGAGCGA ATGGTCGCCG TGGCCAGCTC CAGCGGCAGC 
CTGAAGAAGA TGCTCGCCAG CTGGGCCAAG GGCATCACGC TGAAGCACTA CATGGTGAGT 
CAAGGCAAGA GCTCCGGGGG ATTCCGGTAC AAGATTGCCA AGTCGCTCAT CATGTCCAAG 
GTGAAGCAGG CCCTGGGCTT CGATCGCGTC CTTACACTGG CCAGTGCGGC AGCTCCCATG 
TCGCCGGAGA CGAAGAAGTA CTTCCTCAGT CTGGACCTAA AGATTGTCGA TGCCTTCGGC 
ATGTCAGAAA CGGCCGGTTG TCACACCATC TGCCTTCCCG ATTCCGTGGG TCTGAACACA 
ATCGGCAAAA CTTTGCCCGG CTGCGAGTCC AAGTTCATCA ACAAGGATGC CAACGGTCAC 
GGAGAGCTGT GCATCCGAGG ACGTCACGTT TTCATGGGCT ACATCGACAA CAAGGAGAAG 
ACCGAGGAGT CGCTGGATGA CGACTGCTGG CTGCATTCCG GTGATTTGGG ATTTGTGGAT 
GACAAGGGTT ATGTTTCACT GACGGGACGA TCCAAGGAGA TCATCATTAC CGCCGGCGGC 
GAGAACATAC CGCCAGTGCA CATCGAGAAC ACGATCAAGA AGGAGCTGGA TGCCATTTCC 
AATGCCTTTT TGGTGGGCGA GCAGCGCAAA TATCTCACTG TTCTGATCAC CCTAAAGACC 
GAAGTGGACA AGGATTCCGG TGAGCCGCTG GACGAGCTTA GCCACGAGTC CTCCGTGTGG 
GTGAAATCGC TGGGAGTGGA GCACAAGACC GTATCGGATA TCCTGGCCGC AGGTCCCTGC 
CCCAAGGTGT GGAAGTCCAT CGAGGATGCC ATTAAGCGGG CCAACAAGCA GTCCATTTCC 
AATGCCCAAA AGGTTCAGAA GTTCACCATT CTGCCGCACG ACTTCTCCAT TCCCACCGGC 
GAACTTGGAC CCACCCTAAA GGTTAAGCGC AACGTTGTGT CCAAGATGTA TGCCGATGAG 
ATCGAGAAAC TATATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524698.1
CDS	182..2182
Translation	MSTIDALYNRPGPNRLRQADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKIHAIRDKLPKLKAAIQIQEPYSPYLKKEDGYYRWSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQGKSSGGFRYKIAKSLIMSKVKQALGFDRVLTLASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTICLPDSVGLNTIGKTLPGCESKFINKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKELDAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSDILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYADEIEKLYA

Target ORF information:

RefSeq Version	NM_079959.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bubblegum (bgm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079959.4

ORF Insert Sequence:

ATGTCCACGA TAGACGCGCT CTACAATCGT CCTGGGCCCA ACCGCCTGCG GCAGGCGGAT 
GCCTATCGCA CCACCAATCG TCAGGATGCC GTCAAGATTC GTATGGCCAA GGATGGAATC 
GGCGCAGAGG AGCCCATCTC CGTGCCCGGC CTGCTGAAGC GTACGGTCAA CAATTATGGC 
GACTATCCTG CGCTGCGCAC CAAGAACGGC AAGAACGGAT ATCACACTGT CACCTACAAA 
CAATATGAGC AGAAGGTGCA CCAGGTGGCC AAGGCGTTCA TTAAGCTCGG TCTGGAGGAG 
CACCATTCGG TGGGTGTGCT GGCCTTCAAT TGCGCCGAAT GGTTCTACTC GGCCATGGGC 
GCCATTCACG CACGAGGCAT CATCGCCGGA ATCTACACCA CCAATTCCGC CGATGCAGTG 
CAGCACGTTC TGGAGAGCTC ACATGCCCAA ATCGTGGTCG TCGACGACGC CAAGCAAATG 
GACAAGATTC ACGCCATTCG CGACAAGCTG CCCAAGCTCA AGGCCGCCAT TCAGATCCAG 
GAGCCCTATT CCCCCTACTT GAAGAAGGAG GATGGCTACT ACAGGTGGTC GGAGATCGAG 
TCGATGAACG TTAGCGACGT GGAGGATCAG TACATGACCC GTTTGGAGAA TGTGGCGATC 
AACGAGTGCT GCTGCCTGGT CTACACCTCC GGAACGGTGG GCATGCCCAA GGGCGTGATG 
CTCTCCCACG ACAACATCAC CTTCGATGTG CGCGGCATCG TCAAGGCCAT GGACCGTGTG 
GTGGTTGGGG CGGAGTCGAT CGTCTCCTAC CTGCCACTTT CGCACGTGGC CGCCCAGACC 
GTGGACATTT ACACCTGCGC CTTTGTGGCG GGCTGCATTT GGTTCGCCGA CAAGGATGCG 
CTGAAGGGAA CGCTGGTGAA GTCGTTGCAG GATGCGCGAC CCACGCGATT CATGGGCGTG 
CCGCGTGTGT ACGAGAAGTT CCAGGAGCGA ATGGTCGCCG TGGCCAGCTC CAGCGGCAGC 
CTGAAGAAGA TGCTCGCCAG CTGGGCCAAG GGCATCACGC TGAAGCACTA CATGGTGAGT 
CAAGGCAAGA GCTCCGGGGG ATTCCGGTAC AAGATTGCCA AGTCGCTCAT CATGTCCAAG 
GTGAAGCAGG CCCTGGGCTT CGATCGCGTC CTTACACTGG CCAGTGCGGC AGCTCCCATG 
TCGCCGGAGA CGAAGAAGTA CTTCCTCAGT CTGGACCTAA AGATTGTCGA TGCCTTCGGC 
ATGTCAGAAA CGGCCGGTTG TCACACCATC TGCCTTCCCG ATTCCGTGGG TCTGAACACA 
ATCGGCAAAA CTTTGCCCGG CTGCGAGTCC AAGTTCATCA ACAAGGATGC CAACGGTCAC 
GGAGAGCTGT GCATCCGAGG ACGTCACGTT TTCATGGGCT ACATCGACAA CAAGGAGAAG 
ACCGAGGAGT CGCTGGATGA CGACTGCTGG CTGCATTCCG GTGATTTGGG ATTTGTGGAT 
GACAAGGGTT ATGTTTCACT GACGGGACGA TCCAAGGAGA TCATCATTAC CGCCGGCGGC 
GAGAACATAC CGCCAGTGCA CATCGAGAAC ACGATCAAGA AGGAGCTGGA TGCCATTTCC 
AATGCCTTTT TGGTGGGCGA GCAGCGCAAA TATCTCACTG TTCTGATCAC CCTAAAGACC 
GAAGTGGACA AGGATTCCGG TGAGCCGCTG GACGAGCTTA GCCACGAGTC CTCCGTGTGG 
GTGAAATCGC TGGGAGTGGA GCACAAGACC GTATCGGATA TCCTGGCCGC AGGTCCCTGC 
CCCAAGGTGT GGAAGTCCAT CGAGGATGCC ATTAAGCGGG CCAACAAGCA GTCCATTTCC 
AATGCCCAAA AGGTTCAGAA GTTCACCATT CTGCCGCACG ACTTCTCCAT TCCCACCGGC 
GAACTTGGAC CCACCCTAAA GGTTAAGCGC AACGTTGTGT CCAAGATGTA TGCCGATGAG 
ATCGAGAAAC TATATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bgm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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