Men-b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Men-b
Entrez Gene ID	43936
Full Name	Malic enzyme b
Synonyms	CG5889,Dmel\CG5889,MDH,Mdh,anon-EST:Posey79
General protein information	Preferred Names Malic enzyme b Names CG5889 gene product from transcript CG5889-RC CG5889-PA CG5889-PB CG5889-PC Mdh Men-b-PA Men-b-PB Men-b-PC malic DH malic enzyme b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97E5-97E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170278.2	NP_001163749.1	malic enzyme b, isoform B
NM_001170279.2	NP_001163750.1	malic enzyme b, isoform C
NM_143702.4	NP_651959.1	malic enzyme b, isoform A

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os01g0743500	NP_001044219.1
Homo sapiens (human)	ME3	NP_006671.2
Pan troglodytes (chimpanzee)	ME3	XP_508682.2
Mus musculus (house mouse)	Me3	NP_852072.2
Gallus gallus (chicken)	ME3	XP_003640618.2
Canis lupus familiaris (dog)	ME3	XP_005633441.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004159	XP_003436731.1
Macaca mulatta (Rhesus monkey)	ME3	XP_001103502.1
Bos taurus (cattle)	ME3	NP_001069345.1
Danio rerio (zebrafish)	me3	NP_001082825.1
Drosophila melanogaster (fruit fly)	Men-b	NP_651959.1
Xenopus tropicalis (tropical clawed frog)	me3	NP_001107309.1
Rattus norvegicus (Norway rat)	Me3	NP_001101961.2
Caenorhabditis elegans (roundworm)	men-1	NP_496968.1
Arabidopsis thaliana (thale cress)	NADP-ME1	NP_179580.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Men-b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Men-b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06822	NM_143702.4 Latest version!	Drosophila melanogaster malic enzyme b, transcript variant A (Men-b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa07763	NM_001170279.2 Latest version!	Drosophila melanogaster malic enzyme b, transcript variant C (Men-b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa07764	NM_001170278.2 Latest version!	Drosophila melanogaster malic enzyme b, transcript variant B (Men-b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Men-b ( NM_143702.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651959.1 Drosophila melanogaster malic enzyme b, transcript variant A (Men-b), mRNA.

Price & Availability↑

CloneID	OFa06822
Clone ID Related Accession (Same CDS sequence)	NM_143702.4
Accession Version	NM_143702.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	malic enzyme b, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143702.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTCAC GACCCAGCTT ATGTGGAACT GTGGGCAAAT TGTGTCGATG CGGCACTTCA 
GCAACAGGAA AGACTGCTGT GGCAGCAGCA ACAGTTCCCA CCGCTCGACA CTATCATGAG 
GTGGTGGGCG ATATCATCTG CCCGTCTCAG GTGCGCGGCA TAGATCACAT CCGTGATCCG 
CGACTCAACA AGGGCCTTGC CTTTACACTG GAGGAGCGCC AGACTCTGGG CATCCATGGG 
CTGCAGCCAG CGCGCTTCAA GACGCAAGAG GAGCAGCTGC AGCTATGCAA GATCGCCGTA 
AACCGCTACA CGGAGCCGCT GAACAAGTAT CTCTATCTGA GCGATCTGTA CGATCGCAAT 
GAGCGTCTGT TCTTCCGCTT CCTATCGGAG AACATCGAGG ATCTGATGCC CATTGTGTAC 
ACGCCGACGG TGGGCTTGGC CTGCCAGCGC TTTGGCCTGA TCTACCGACG TCCTCATGGT 
CTGTTCATCA CCTACAACGA TCGAGGACAC ATCTTTGATG TGATGAAGAA CTGGCCGGAG 
CCGAATGTGC GTGCCATCTG TGTGACGGAT GGTGAGCGCA TCCTGGGACT GGGAGATTTG 
GGCGCCTGCG GCATGGGCAT TCCCGTGGGC AAGCTGGCCT TGTACACGGC TCTGGCCGGA 
ATCAAGCCCC ATCAGTGCCT GCCCATTGTG GTGGATGTGG GCACCAACAA CATCGATCTG 
CTGGAGGATC CCCTGTACGT GGGTCTGCGT CAGAAGCGAG TGGTTGGTCG GGAGTACGAC 
GAGTTCATTG ACGAGTTTAT GGAGGCCGTG GTGCAGCGCT ATGGCCAGAA CACTCTGATC 
CAGTTTGAGG ACTTTGGCAA CCACAATGCA TTTAGGTTCC TGGACAAATA CCGCAACACC 
TATTGCACTT TCAACGACGA CATCCAGGGA ACCGCCTCCG TGGCCGTTGC TGGGCTCTAT 
GCCTCCAAGC GTATTACGGG TAAGTCCTTC AAGGACTACA CCTTCCTGTT TGCAGGAGCC 
GGTGAGGCCG CCATCGGCAT CGCCGATCTC ACGGTCAAGG CCATGGTGCA GGATGGTGTG 
CCCATCGAGG AGGCCTACAA CAGAATTTAC ATGGTCGATA TCGATGGTCT GCTGACCAAG 
AGCCGCAAGG TGGGCAACTT GGATGGCCAC AAAATCCACT ACGCCAAGGA CATCAATCCG 
ATGTCAGATC TCGCCGAGAT TGTCTCCACC ATCAAACCCA GTGTGCTTAT TGGTGCCTCG 
GCTGCTGCCG GCATTTTCAC ACCGGAAATC CTGCGCACCA TGGCGGATAA CAATGAGAGG 
CCCGTGGTGT TCGCCTTGTC CAATCCCACC AGCAAGGCAG AATGCACTGC CGAAGATGCT 
TACAAGCACA CGGATGCTCG CGTGATCTTC TCGTCGGGCT CTCCCTTCCC ACCGGTCCAG 
ATCGGTGACA AGACCTTCTA CCCGGGCCAG GGCAACAATG CCTACATCTT CCCAGGCGTT 
GGCTTGGGCG TGATCTGCAC GGGTACTCAC CACATACCCG ACGAAATGTT CCTCATCGCC 
GCCCAGGAGT TGGCCAACTT CGTGGAGCCC AGCGATATTG AGCGCGGATC GCTGTATCCT 
CCGCTGTCGA GCATCCGCAA CGTGTCCATG AACATTGCCG TTGGCGTGAC CAAATGTGCC 
TACGATAGAG GCTTGGCATC TACCTACCCT GAGCCACAGG ACAAGCGCAA GTGGCTGGAG 
AATCAGCTGT ACAACTTCAA CTACGAGAGC TCGATGCCCG CTTCCTGGGT TTGGCCGCGG 
ATGCCCTACA TTAAGACTCG CGAAGAAAGC CCGCTCATTG CCGCCATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651959.1
CDS	229..2082
Translation	MFSRPSLCGTVGKLCRCGTSATGKTAVAAATVPTARHYHEVVGDIICPSQVRGIDHIRDPRLNKGLAFTLEERQTLGIHGLQPARFKTQEEQLQLCKIAVNRYTEPLNKYLYLSDLYDRNERLFFRFLSENIEDLMPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERILGLGDLGACGMGIPVGKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKRVVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYCTFNDDIQGTASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDGVPIEEAYNRIYMVDIDGLLTKSRKVGNLDGHKIHYAKDINPMSDLAEIVSTIKPSVLIGASAAAGIFTPEILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYPGQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFVEPSDIERGSLYPPLSSIRNVSMNIAVGVTKCAYDRGLASTYPEPQDKRKWLENQLYNFNYESSMPASWVWPRMPYIKTREESPLIAAIK

Target ORF information:

RefSeq Version	NM_143702.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malic enzyme b, transcript variant A (Men-b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143702.4

ORF Insert Sequence:

ATGTTCTCAC GACCCAGCTT ATGTGGAACT GTGGGCAAAT TGTGTCGATG CGGCACTTCA 
GCAACAGGAA AGACTGCTGT GGCAGCAGCA ACAGTTCCCA CCGCTCGACA CTATCATGAG 
GTGGTGGGCG ATATCATCTG CCCGTCTCAG GTGCGCGGCA TAGATCACAT CCGTGATCCG 
CGACTCAACA AGGGCCTTGC CTTTACACTG GAGGAGCGCC AGACTCTGGG CATCCATGGG 
CTGCAGCCAG CGCGCTTCAA GACGCAAGAG GAGCAGCTGC AGCTATGCAA GATCGCCGTA 
AACCGCTACA CGGAGCCGCT GAACAAGTAT CTCTATCTGA GCGATCTGTA CGATCGCAAT 
GAGCGTCTGT TCTTCCGCTT CCTATCGGAG AACATCGAGG ATCTGATGCC CATTGTGTAC 
ACGCCGACGG TGGGCTTGGC CTGCCAGCGC TTTGGCCTGA TCTACCGACG TCCTCATGGT 
CTGTTCATCA CCTACAACGA TCGAGGACAC ATCTTTGATG TGATGAAGAA CTGGCCGGAG 
CCGAATGTGC GTGCCATCTG TGTGACGGAT GGTGAGCGCA TCCTGGGACT GGGAGATTTG 
GGCGCCTGCG GCATGGGCAT TCCCGTGGGC AAGCTGGCCT TGTACACGGC TCTGGCCGGA 
ATCAAGCCCC ATCAGTGCCT GCCCATTGTG GTGGATGTGG GCACCAACAA CATCGATCTG 
CTGGAGGATC CCCTGTACGT GGGTCTGCGT CAGAAGCGAG TGGTTGGTCG GGAGTACGAC 
GAGTTCATTG ACGAGTTTAT GGAGGCCGTG GTGCAGCGCT ATGGCCAGAA CACTCTGATC 
CAGTTTGAGG ACTTTGGCAA CCACAATGCA TTTAGGTTCC TGGACAAATA CCGCAACACC 
TATTGCACTT TCAACGACGA CATCCAGGGA ACCGCCTCCG TGGCCGTTGC TGGGCTCTAT 
GCCTCCAAGC GTATTACGGG TAAGTCCTTC AAGGACTACA CCTTCCTGTT TGCAGGAGCC 
GGTGAGGCCG CCATCGGCAT CGCCGATCTC ACGGTCAAGG CCATGGTGCA GGATGGTGTG 
CCCATCGAGG AGGCCTACAA CAGAATTTAC ATGGTCGATA TCGATGGTCT GCTGACCAAG 
AGCCGCAAGG TGGGCAACTT GGATGGCCAC AAAATCCACT ACGCCAAGGA CATCAATCCG 
ATGTCAGATC TCGCCGAGAT TGTCTCCACC ATCAAACCCA GTGTGCTTAT TGGTGCCTCG 
GCTGCTGCCG GCATTTTCAC ACCGGAAATC CTGCGCACCA TGGCGGATAA CAATGAGAGG 
CCCGTGGTGT TCGCCTTGTC CAATCCCACC AGCAAGGCAG AATGCACTGC CGAAGATGCT 
TACAAGCACA CGGATGCTCG CGTGATCTTC TCGTCGGGCT CTCCCTTCCC ACCGGTCCAG 
ATCGGTGACA AGACCTTCTA CCCGGGCCAG GGCAACAATG CCTACATCTT CCCAGGCGTT 
GGCTTGGGCG TGATCTGCAC GGGTACTCAC CACATACCCG ACGAAATGTT CCTCATCGCC 
GCCCAGGAGT TGGCCAACTT CGTGGAGCCC AGCGATATTG AGCGCGGATC GCTGTATCCT 
CCGCTGTCGA GCATCCGCAA CGTGTCCATG AACATTGCCG TTGGCGTGAC CAAATGTGCC 
TACGATAGAG GCTTGGCATC TACCTACCCT GAGCCACAGG ACAAGCGCAA GTGGCTGGAG 
AATCAGCTGT ACAACTTCAA CTACGAGAGC TCGATGCCCG CTTCCTGGGT TTGGCCGCGG 
ATGCCCTACA TTAAGACTCG CGAAGAAAGC CCGCTCATTG CCGCCATCAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Men-b ( NM_001170279.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163750.1 Drosophila melanogaster malic enzyme b, transcript variant C (Men-b), mRNA.

Price & Availability↑

CloneID	OFa07763
Clone ID Related Accession (Same CDS sequence)	NM_001170279.2
Accession Version	NM_001170279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	malic enzyme b, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170279.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTCAC GACCCAGCTT ATGTGGAACT GTGGGCAAAT TGTGTCGATG CGGCACTTCA 
GCAACAGGAA AGACTGCTGT GGCAGCAGCA ACAGTTCCCA CCGCTCGACA CTATCATGAG 
GTGGTGGGCG ATATCATCTG CCCGTCTCAG GTGCGCGGCA TAGATCACAT CCGTGATCCG 
CGACTCAACA AGGGCCTTGC CTTTACACTG GAGGAGCGCC AGACTCTGGG CATCCATGGG 
CTGCAGCCAG CGCGCTTCAA GACGCAAGAG GAGCAGCTGC AGCTATGCAA GATCGCCGTA 
AACCGCTACA CGGAGCCGCT GAACAAGTAT CTCTATCTGA GCGATCTGTA CGATCGCAAT 
GAGCGTCTGT TCTTCCGCTT CCTATCGGAG AACATCGAGG ATCTGATGCC CATTGTGTAC 
ACGCCGACGG TGGGCTTGGC CTGCCAGCGC TTTGGCCTGA TCTACCGACG TCCTCATGGT 
CTGTTCATCA CCTACAACGA TCGAGGACAC ATCTTTGATG TGATGAAGAA CTGGCCGGAG 
CCGAATGTGC GTGCCATCTG TGTGACGGAT GGTGAGCGCA TCCTGGGACT GGGAGATTTG 
GGCGCCTGCG GCATGGGCAT TCCCGTGGGC AAGCTGGCCT TGTACACGGC TCTGGCCGGA 
ATCAAGCCCC ATCAGTGCCT GCCCATTGTG GTGGATGTGG GCACCAACAA CATCGATCTG 
CTGGAGGATC CCCTGTACGT GGGTCTGCGT CAGAAGCGAG TGGTTGGTCG GGAGTACGAC 
GAGTTCATTG ACGAGTTTAT GGAGGCCGTG GTGCAGCGCT ATGGCCAGAA CACTCTGATC 
CAGTTTGAGG ACTTTGGCAA CCACAATGCA TTTAGGTTCC TGGACAAATA CCGCAACACC 
TATTGCACTT TCAACGACGA CATCCAGGGA ACCGCCTCCG TGGCCGTTGC TGGGCTCTAT 
GCCTCCAAGC GTATTACGGG TAAGTCCTTC AAGGACTACA CCTTCCTGTT TGCAGGAGCC 
GGTGAGGCCG CCATCGGCAT CGCCGATCTC ACGGTCAAGG CCATGGTGCA GGATGGTGTG 
CCCATCGAGG AGGCCTACAA CAGAATTTAC ATGGTCGATA TCGATGGTCT GCTGACCAAG 
AGCCGCAAGG TGGGCAACTT GGATGGCCAC AAAATCCACT ACGCCAAGGA CATCAATCCG 
ATGTCAGATC TCGCCGAGAT TGTCTCCACC ATCAAACCCA GTGTGCTTAT TGGTGCCTCG 
GCTGCTGCCG GCATTTTCAC ACCGGAAATC CTGCGCACCA TGGCGGATAA CAATGAGAGG 
CCCGTGGTGT TCGCCTTGTC CAATCCCACC AGCAAGGCAG AATGCACTGC CGAAGATGCT 
TACAAGCACA CGGATGCTCG CGTGATCTTC TCGTCGGGCT CTCCCTTCCC ACCGGTCCAG 
ATCGGTGACA AGACCTTCTA CCCGGGCCAG GGCAACAATG CCTACATCTT CCCAGGCGTT 
GGCTTGGGCG TGATCTGCAC GGGTACTCAC CACATACCCG ACGAAATGTT CCTCATCGCC 
GCCCAGGAGT TGGCCAACTT CGTGGAGCCC AGCGATATTG AGCGCGGATC GCTGTATCCT 
CCGCTGTCGA GCATCCGCAA CGTGTCCATG AACATTGCCG TTGGCGTGAC CAAATGTGCC 
TACGATAGAG GCTTGGCATC TACCTACCCT GAGCCACAGG ACAAGCGCAA GTGGCTGGAG 
AATCAGCTGT ACAACTTCAA CTACGAGAGC TCGATGCCCG CTTCCTGGGT TTGGCCGCGG 
ATGCCCTACA TTAAGACTCG TGATTTAGTC CCCACAAAGC TCTATGGAAA ACAAAAGAGC 
GAGGAAGTAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163750.1
CDS	229..2103
Translation	MFSRPSLCGTVGKLCRCGTSATGKTAVAAATVPTARHYHEVVGDIICPSQVRGIDHIRDPRLNKGLAFTLEERQTLGIHGLQPARFKTQEEQLQLCKIAVNRYTEPLNKYLYLSDLYDRNERLFFRFLSENIEDLMPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERILGLGDLGACGMGIPVGKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKRVVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYCTFNDDIQGTASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDGVPIEEAYNRIYMVDIDGLLTKSRKVGNLDGHKIHYAKDINPMSDLAEIVSTIKPSVLIGASAAAGIFTPEILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYPGQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFVEPSDIERGSLYPPLSSIRNVSMNIAVGVTKCAYDRGLASTYPEPQDKRKWLENQLYNFNYESSMPASWVWPRMPYIKTRDLVPTKLYGKQKSEEVM

Target ORF information:

RefSeq Version	NM_001170279.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malic enzyme b, transcript variant C (Men-b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170279.2

ORF Insert Sequence:

ATGTTCTCAC GACCCAGCTT ATGTGGAACT GTGGGCAAAT TGTGTCGATG CGGCACTTCA 
GCAACAGGAA AGACTGCTGT GGCAGCAGCA ACAGTTCCCA CCGCTCGACA CTATCATGAG 
GTGGTGGGCG ATATCATCTG CCCGTCTCAG GTGCGCGGCA TAGATCACAT CCGTGATCCG 
CGACTCAACA AGGGCCTTGC CTTTACACTG GAGGAGCGCC AGACTCTGGG CATCCATGGG 
CTGCAGCCAG CGCGCTTCAA GACGCAAGAG GAGCAGCTGC AGCTATGCAA GATCGCCGTA 
AACCGCTACA CGGAGCCGCT GAACAAGTAT CTCTATCTGA GCGATCTGTA CGATCGCAAT 
GAGCGTCTGT TCTTCCGCTT CCTATCGGAG AACATCGAGG ATCTGATGCC CATTGTGTAC 
ACGCCGACGG TGGGCTTGGC CTGCCAGCGC TTTGGCCTGA TCTACCGACG TCCTCATGGT 
CTGTTCATCA CCTACAACGA TCGAGGACAC ATCTTTGATG TGATGAAGAA CTGGCCGGAG 
CCGAATGTGC GTGCCATCTG TGTGACGGAT GGTGAGCGCA TCCTGGGACT GGGAGATTTG 
GGCGCCTGCG GCATGGGCAT TCCCGTGGGC AAGCTGGCCT TGTACACGGC TCTGGCCGGA 
ATCAAGCCCC ATCAGTGCCT GCCCATTGTG GTGGATGTGG GCACCAACAA CATCGATCTG 
CTGGAGGATC CCCTGTACGT GGGTCTGCGT CAGAAGCGAG TGGTTGGTCG GGAGTACGAC 
GAGTTCATTG ACGAGTTTAT GGAGGCCGTG GTGCAGCGCT ATGGCCAGAA CACTCTGATC 
CAGTTTGAGG ACTTTGGCAA CCACAATGCA TTTAGGTTCC TGGACAAATA CCGCAACACC 
TATTGCACTT TCAACGACGA CATCCAGGGA ACCGCCTCCG TGGCCGTTGC TGGGCTCTAT 
GCCTCCAAGC GTATTACGGG TAAGTCCTTC AAGGACTACA CCTTCCTGTT TGCAGGAGCC 
GGTGAGGCCG CCATCGGCAT CGCCGATCTC ACGGTCAAGG CCATGGTGCA GGATGGTGTG 
CCCATCGAGG AGGCCTACAA CAGAATTTAC ATGGTCGATA TCGATGGTCT GCTGACCAAG 
AGCCGCAAGG TGGGCAACTT GGATGGCCAC AAAATCCACT ACGCCAAGGA CATCAATCCG 
ATGTCAGATC TCGCCGAGAT TGTCTCCACC ATCAAACCCA GTGTGCTTAT TGGTGCCTCG 
GCTGCTGCCG GCATTTTCAC ACCGGAAATC CTGCGCACCA TGGCGGATAA CAATGAGAGG 
CCCGTGGTGT TCGCCTTGTC CAATCCCACC AGCAAGGCAG AATGCACTGC CGAAGATGCT 
TACAAGCACA CGGATGCTCG CGTGATCTTC TCGTCGGGCT CTCCCTTCCC ACCGGTCCAG 
ATCGGTGACA AGACCTTCTA CCCGGGCCAG GGCAACAATG CCTACATCTT CCCAGGCGTT 
GGCTTGGGCG TGATCTGCAC GGGTACTCAC CACATACCCG ACGAAATGTT CCTCATCGCC 
GCCCAGGAGT TGGCCAACTT CGTGGAGCCC AGCGATATTG AGCGCGGATC GCTGTATCCT 
CCGCTGTCGA GCATCCGCAA CGTGTCCATG AACATTGCCG TTGGCGTGAC CAAATGTGCC 
TACGATAGAG GCTTGGCATC TACCTACCCT GAGCCACAGG ACAAGCGCAA GTGGCTGGAG 
AATCAGCTGT ACAACTTCAA CTACGAGAGC TCGATGCCCG CTTCCTGGGT TTGGCCGCGG 
ATGCCCTACA TTAAGACTCG TGATTTAGTC CCCACAAAGC TCTATGGAAA ACAAAAGAGC 
GAGGAAGTAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Men-b ( NM_001170278.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163749.1 Drosophila melanogaster malic enzyme b, transcript variant B (Men-b), mRNA.

Price & Availability↑

CloneID	OFa07764
Clone ID Related Accession (Same CDS sequence)	NM_001170278.2
Accession Version	NM_001170278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	malic enzyme b, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170278.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTCAC GACCCAGCTT ATGTGGAACT GTGGGCAAAT TGTGTCGATG CGGCACTTCA 
GCAACAGGAA AGACTGCTGT GGCAGCAGCA ACAGTTCCCA CCGCTCGACA CTATCATGAG 
GTGGTGGGCG ATATCATCTG CCCGTCTCAG GTGCGCGGCA TAGATCACAT CCGTGATCCG 
CGACTCAACA AGGGCCTTGC CTTTACACTG GAGGAGCGCC AGACTCTGGG CATCCATGGG 
CTGCAGCCAG CGCGCTTCAA GACGCAAGAG GAGCAGCTGC AGCTATGCAA GATCGCCGTA 
AACCGCTACA CGGAGCCGCT GAACAAGTAT CTCTATCTGA GCGATCTGTA CGATCGCAAT 
GAGCGTCTGT TCTTCCGCTT CCTATCGGAG AACATCGAGG ATCTGATGCC CATTGTGTAC 
ACGCCGACGG TGGGCTTGGC CTGCCAGCGC TTTGGCCTGA TCTACCGACG TCCTCATGGT 
CTGTTCATCA CCTACAACGA TCGAGGACAC ATCTTTGATG TGATGAAGAA CTGGCCGGAG 
CCGAATGTGC GTGCCATCTG TGTGACGGAT GGTGAGCGCA TCCTGGGACT GGGAGATTTG 
GGCGCCTGCG GCATGGGCAT TCCCGTGGGC AAGCTGGCCT TGTACACGGC TCTGGCCGGA 
ATCAAGCCCC ATCAGTGCCT GCCCATTGTG GTGGATGTGG GCACCAACAA CATCGATCTG 
CTGGAGGATC CCCTGTACGT GGGTCTGCGT CAGAAGCGAG TGGTTGGTCG GGAGTACGAC 
GAGTTCATTG ACGAGTTTAT GGAGGCCGTG GTGCAGCGCT ATGGCCAGAA CACTCTGATC 
CAGTTTGAGG ACTTTGGCAA CCACAATGCA TTTAGGTTCC TGGACAAATA CCGCAACACC 
TATTGCACTT TCAACGACGA CATCCAGGGA ACCGCCTCCG TGGCCGTTGC TGGGCTCTAT 
GCCTCCAAGC GTATTACGGG TAAGTCCTTC AAGGACTACA CCTTCCTGTT TGCAGGAGCC 
GGTGAGGCCG CCATCGGCAT CGCCGATCTC ACGGTCAAGG CCATGGTGCA GGATGGTGTG 
CCCATCGAGG AGGCCTACAA CAGAATTTAC ATGGTCGATA TCGATGGTCT GCTGACCAAG 
AGCCGCAAGG TGGGCAACTT GGATGGCCAC AAAATCCACT ACGCCAAGGA CATCAATCCG 
ATGTCAGATC TCGCCGAGAT TGTCTCCACC ATCAAACCCA GTGTGCTTAT TGGTGCCTCG 
GCTGCTGCCG GCATTTTCAC ACCGGAAATC CTGCGCACCA TGGCGGATAA CAATGAGAGG 
CCCGTGGTGT TCGCCTTGTC CAATCCCACC AGCAAGGCAG AATGCACTGC CGAAGATGCT 
TACAAGCACA CGGATGCTCG CGTGATCTTC TCGTCGGGCT CTCCCTTCCC ACCGGTCCAG 
ATCGGTGACA AGACCTTCTA CCCGGGCCAG GGCAACAATG CCTACATCTT CCCAGGCGTT 
GGCTTGGGCG TGATCTGCAC GGGTACTCAC CACATACCCG ACGAAATGTT CCTCATCGCC 
GCCCAGGAGT TGGCCAACTT CGTGGAGCCC AGCGATATTG AGCGCGGATC GCTGTATCCT 
CCGCTGTCGA GCATCCGCAA CGTGTCCATG AACATTGCCG TTGGCGTGAC CAAATGTGCC 
TACGATAGAG GCTTGGCATC TACCTACCCT GAGCCACAGG ACAAGCGCAA GTGGCTGGAG 
AATCAGCTGT ACAACTTCAA CTACGAGAGC TCGATGCCCG CTTCCTGGGT TTGGCCGCGG 
ATGCCCTACA TTAAGACTCG TGATTTAGTC CCCACAAAGC TCTATGGAAA ACAAAGTGAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163749.1
CDS	229..2094
Translation	MFSRPSLCGTVGKLCRCGTSATGKTAVAAATVPTARHYHEVVGDIICPSQVRGIDHIRDPRLNKGLAFTLEERQTLGIHGLQPARFKTQEEQLQLCKIAVNRYTEPLNKYLYLSDLYDRNERLFFRFLSENIEDLMPIVYTPTVGLACQRFGLIYRRPHGLFITYNDRGHIFDVMKNWPEPNVRAICVTDGERILGLGDLGACGMGIPVGKLALYTALAGIKPHQCLPIVVDVGTNNIDLLEDPLYVGLRQKRVVGREYDEFIDEFMEAVVQRYGQNTLIQFEDFGNHNAFRFLDKYRNTYCTFNDDIQGTASVAVAGLYASKRITGKSFKDYTFLFAGAGEAAIGIADLTVKAMVQDGVPIEEAYNRIYMVDIDGLLTKSRKVGNLDGHKIHYAKDINPMSDLAEIVSTIKPSVLIGASAAAGIFTPEILRTMADNNERPVVFALSNPTSKAECTAEDAYKHTDARVIFSSGSPFPPVQIGDKTFYPGQGNNAYIFPGVGLGVICTGTHHIPDEMFLIAAQELANFVEPSDIERGSLYPPLSSIRNVSMNIAVGVTKCAYDRGLASTYPEPQDKRKWLENQLYNFNYESSMPASWVWPRMPYIKTRDLVPTKLYGKQSEN

Target ORF information:

RefSeq Version	NM_001170278.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malic enzyme b, transcript variant B (Men-b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170278.2

ORF Insert Sequence:

ATGTTCTCAC GACCCAGCTT ATGTGGAACT GTGGGCAAAT TGTGTCGATG CGGCACTTCA 
GCAACAGGAA AGACTGCTGT GGCAGCAGCA ACAGTTCCCA CCGCTCGACA CTATCATGAG 
GTGGTGGGCG ATATCATCTG CCCGTCTCAG GTGCGCGGCA TAGATCACAT CCGTGATCCG 
CGACTCAACA AGGGCCTTGC CTTTACACTG GAGGAGCGCC AGACTCTGGG CATCCATGGG 
CTGCAGCCAG CGCGCTTCAA GACGCAAGAG GAGCAGCTGC AGCTATGCAA GATCGCCGTA 
AACCGCTACA CGGAGCCGCT GAACAAGTAT CTCTATCTGA GCGATCTGTA CGATCGCAAT 
GAGCGTCTGT TCTTCCGCTT CCTATCGGAG AACATCGAGG ATCTGATGCC CATTGTGTAC 
ACGCCGACGG TGGGCTTGGC CTGCCAGCGC TTTGGCCTGA TCTACCGACG TCCTCATGGT 
CTGTTCATCA CCTACAACGA TCGAGGACAC ATCTTTGATG TGATGAAGAA CTGGCCGGAG 
CCGAATGTGC GTGCCATCTG TGTGACGGAT GGTGAGCGCA TCCTGGGACT GGGAGATTTG 
GGCGCCTGCG GCATGGGCAT TCCCGTGGGC AAGCTGGCCT TGTACACGGC TCTGGCCGGA 
ATCAAGCCCC ATCAGTGCCT GCCCATTGTG GTGGATGTGG GCACCAACAA CATCGATCTG 
CTGGAGGATC CCCTGTACGT GGGTCTGCGT CAGAAGCGAG TGGTTGGTCG GGAGTACGAC 
GAGTTCATTG ACGAGTTTAT GGAGGCCGTG GTGCAGCGCT ATGGCCAGAA CACTCTGATC 
CAGTTTGAGG ACTTTGGCAA CCACAATGCA TTTAGGTTCC TGGACAAATA CCGCAACACC 
TATTGCACTT TCAACGACGA CATCCAGGGA ACCGCCTCCG TGGCCGTTGC TGGGCTCTAT 
GCCTCCAAGC GTATTACGGG TAAGTCCTTC AAGGACTACA CCTTCCTGTT TGCAGGAGCC 
GGTGAGGCCG CCATCGGCAT CGCCGATCTC ACGGTCAAGG CCATGGTGCA GGATGGTGTG 
CCCATCGAGG AGGCCTACAA CAGAATTTAC ATGGTCGATA TCGATGGTCT GCTGACCAAG 
AGCCGCAAGG TGGGCAACTT GGATGGCCAC AAAATCCACT ACGCCAAGGA CATCAATCCG 
ATGTCAGATC TCGCCGAGAT TGTCTCCACC ATCAAACCCA GTGTGCTTAT TGGTGCCTCG 
GCTGCTGCCG GCATTTTCAC ACCGGAAATC CTGCGCACCA TGGCGGATAA CAATGAGAGG 
CCCGTGGTGT TCGCCTTGTC CAATCCCACC AGCAAGGCAG AATGCACTGC CGAAGATGCT 
TACAAGCACA CGGATGCTCG CGTGATCTTC TCGTCGGGCT CTCCCTTCCC ACCGGTCCAG 
ATCGGTGACA AGACCTTCTA CCCGGGCCAG GGCAACAATG CCTACATCTT CCCAGGCGTT 
GGCTTGGGCG TGATCTGCAC GGGTACTCAC CACATACCCG ACGAAATGTT CCTCATCGCC 
GCCCAGGAGT TGGCCAACTT CGTGGAGCCC AGCGATATTG AGCGCGGATC GCTGTATCCT 
CCGCTGTCGA GCATCCGCAA CGTGTCCATG AACATTGCCG TTGGCGTGAC CAAATGTGCC 
TACGATAGAG GCTTGGCATC TACCTACCCT GAGCCACAGG ACAAGCGCAA GTGGCTGGAG 
AATCAGCTGT ACAACTTCAA CTACGAGAGC TCGATGCCCG CTTCCTGGGT TTGGCCGCGG 
ATGCCCTACA TTAAGACTCG TGATTTAGTC CCCACAAAGC TCTATGGAAA ACAAAGTGAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Men-b cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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