Actbeta cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actbeta
Entrez Gene ID	43826
Full Name	Activin-beta
Synonyms	Act,Act-beta,Activin,CG11062,Dmel\CG11062,act,actbeta,activin-beta,anon-WO02059370.79,beta-Activin,dACT,dAct,dActivin,dact
General protein information	Preferred Names Activin-beta Names CG11062 gene product from transcript CG11062-RA Actbeta-PA CG11062-PA activan activin activin 102F activin beta activin-b dActivin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102F8-102F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_143685.3	NP_651942.2	Activin-beta

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	INHBA	XP_001098514.1
Pan troglodytes (chimpanzee)	INHBA	XP_519063.2
Danio rerio (zebrafish)	inhbaa	NP_570991.1
Bos taurus (cattle)	INHBA	NP_776788.1
Gallus gallus (chicken)	INHBA	NP_990727.1
Canis lupus familiaris (dog)	INHBA	XP_540364.2
Drosophila melanogaster (fruit fly)	Actbeta	NP_651942.2
Xenopus tropicalis (tropical clawed frog)	inhba	XP_002933452.1
Homo sapiens (human)	INHBA	NP_002183.1
Mus musculus (house mouse)	Inhba	NP_032406.1
Rattus norvegicus (Norway rat)	Inhba	NP_058824.1
Danio rerio (zebrafish)	inhbab	NP_001018166.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Actbeta gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actbeta cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00308	NM_143685.3 Latest version!	Drosophila melanogaster Activin-beta (Actbeta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actbeta ( NM_143685.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651942.2 Drosophila melanogaster Activin-beta (Actbeta), mRNA.

Price & Availability↑

CloneID	OFa00308
Clone ID Related Accession (Same CDS sequence)	NM_143685.3
Accession Version	NM_143685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Activin-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_143685.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTTG CCTTCGATTC TAATCATTCG CAATCGGGGG CGCCATTCAA AGGCAGCAGG 
TGTTTCTTTA ATTGTCAATG CATCTGCTGT CGCCAAGGAT GCTGCGTTGT GGTTGTAAAG 
TGCTGTTGCT GCTTTAACTT AAACTGCTGC AACAGCCTTG GCTCCCGGAA GTCATTTCCA 
CAACCCGCTG CAATGCGTAA AAAAGTTGCT GACCTCGAAG TCCTTAGAGT ATCAAGGTTT 
GTGGCGGTTA TTTTAGTGCT GGCTCGGTGG GTTACTGCGG TAGCGACACT CCTGACAAGC 
TGCATACTCC TAGACATATT TTCCGTGCCT GGCCAGTCTG GAGTTGCAGA TAGAAGCCAA 
GCCAGCAGTA GGACAGTGCA CGTCTCGGTT CCTACCACAC CTAATGAAAC TCCCAGTAGC 
ACTTCGGAGA CGAAGCTAAA GTTGCTTTAT GGGTATACAT CGTATGACAT AAATAACGAC 
CAACAGGTAA AGTCCAACAA TTTATGTAGA GTGCTTTGTA AAAGTCGCAA TCGTAAACGA 
CAGCGAAGGA GGCGACGCCG ACGCAATCAC AGACGACGCA GGCACAGATA TACTAAGCGA 
CTTCATCATC TAATGCAAGA TAATATGAGC GGCTTTGAGC AAAGACTTAA TTTTAGCGAT 
GCCAAATGCC AGTCTTTGGA GACAAATTAC GGAACTAATT ATGACTTAGT ACAAGGAGGT 
AAACTATTTA GTCAGTCAGA GAGAAGCCTA CTGGTGTCCC CTTTGAGGGA AATTGAAGCA 
CCTTGGCCAG CGATTCATGG TTCAATGCGT AACTGTTCAA AGATTAAACG CAATAGAGCC 
AATCTTATTT GGCTTCTAAT TGGACTCGTC TGGTTTGAAG TCAAACTTAT AAATTGCAAT 
GGGATCAGCA GTAGTAATTA TTATGCTTCG AATTTGGAGA GTCACAAGGG CTGCACCTTG 
TGCCATGAAA GCGGAAAGCC CAACATATAC ACCGATAAAG ATAATCCACA CACAGACTAC 
AACATCTACA ACAAATACCA TTCCAACAAT AATTTCAACA AAAAAACTAA TCAACCCCAT 
AACAACATTG CACCCAGCGA TGAAGTTCGT TTGGAGTCAA TAAAACGGCA AATTTTGACA 
AAGCTTGGTC TTAGCCACAA GCCTAATGTA TCTCATCCTT TACCAAAGCA ATTTATTTGG 
GAAACAATAT ATCGAGTCGA TGGTGGACGA ATGATACCAA ACAATGCATT TGGGAGCAGT 
GGTAAGAATT TAGATCAGAA GACTATCAAG CTGAGGGCGT TTGCCTCGCC CGGATCACAT 
TTGTTTAATG GCCGAGGTGG AAGGACCGAT CAGAGAAGCG AACGGGATCC CAGTCATCAT 
AAATATCGAT CCCCGTTCGA TTTCACTTTC AATATAAGTA AAAATAATGT ATATGGGAAA 
GTATTGAGAA ATCGTTCTTT AGAACGAATC GATAAAAAAA ACTCATTCTT AAACGGATGG 
ACAGAAAACC GTCAATTAAA AATAAACTCT CAAATTGCAT CTATGCCTAT AGAACTAAAG 
AGCCACCATA ACAGTAGTCC TAAGGAGCTT AAAAGCGGAG CGGTCAGAAA AGTTAATGGC 
ATCAACGGCA CACAAATGAA TGAGAATGCC TTAAAAAAAT CTACATATCC TATAGATATA 
AATCATAGTA TTGATAATAA AACCCATACT GGCAAAAATG GTGAGATGAG CCACAATGAT 
TATGAATATT TTAACGATTA CAGTGTTCAG ACTCATGATA AAAACCGGTA CCATGAAGGT 
CGCTCTAGCA TTGGATATCA GCCAGCGATC CACAACATAG AATATGAAAA TCAGAAAGGA 
CATCACGAAT CTTTTGCTGA TGATCATGAA AATATTGATC ATGAAGACTT CTTTGGAAAT 
ACGCAGGAAA TAATTACATT TGCTGAAGAG GGAACCCAAT ATCGACAATA TCGAATACTC 
GAGTTTTCGG CCCAAAACCG CCGTGTTCCC AGCCAAAAAT TATCCATACG AAGCGCTCAG 
ATTCATATAA GGATAGACAA GCCACATAGT CTTTGGATTG AAAAAGCAAA AAGTTTGCCA 
GAAAAACATT TACTAAACAC TAAACGAAAA TGGGGAGCAA ATAAACCACA TCACAGAATA 
AAAATATGGG TATTTCAGTT ATCAACTTCA ATCAATATTA CGGAGAAGGG AATAGATAAG 
GCTATTATCT TCCGAGCTTC TTTTCAAGTT GACCCGAAAA ATTTAGGATG GCAAAAATTC 
GATTTAACCG ATACAATCCG TGAATGGTAC GGCCATACCA GTCACGAAAA ACTGCGTTTG 
TTGATTGATT GTACTGGATG CGGTGGTCGT TACTCTTTGC ACCTCTTTCA AACTTCGAAA 
CTGAGAGGAA ACTCATCCGA CTACTTGAGT ACGAATCCCA ATCGACCATT TCTCGTACTT 
CATACAGAAT CATCCAGAAC AAGGCGTGTT AGGCGACGCG CCGTAGATTG TGGCGGAGCT 
TTAAATGGAC AGTGCTGTAA AGAATCTTTT TATGTTTCTT TCAAAGCACT AGGTTGGGAT 
GACTGGATTA TTGCACCCAG AGGATATTTT GCAAACTATT GCCGAGGCGA TTGCACGGGC 
TCTTTTAGGA CGCCAGACAC TTTCCAAACA TTTCATGCTC ATTTTATAGA GGAGTATCGG 
AAAATGGGAC TTATGAATGG AATGCGACCG TGTTGCGCCC CAATAAAATT CTCTAGTATG 
TCCTTAATAT ATTATGGTGA TGATGGTATT ATTAAACGAG ACTTGCCAAA AATGGTTGTT 
GATGAGTGTG GTTGCCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651942.2
CDS	331..3171
Translation	MRFAFDSNHSQSGAPFKGSRCFFNCQCICCRQGCCVVVVKCCCCFNLNCCNSLGSRKSFPQPAAMRKKVADLEVLRVSRFVAVILVLARWVTAVATLLTSCILLDIFSVPGQSGVADRSQASSRTVHVSVPTTPNETPSSTSETKLKLLYGYTSYDINNDQQVKSNNLCRVLCKSRNRKRQRRRRRRRNHRRRRHRYTKRLHHLMQDNMSGFEQRLNFSDAKCQSLETNYGTNYDLVQGGKLFSQSERSLLVSPLREIEAPWPAIHGSMRNCSKIKRNRANLIWLLIGLVWFEVKLINCNGISSSNYYASNLESHKGCTLCHESGKPNIYTDKDNPHTDYNIYNKYHSNNNFNKKTNQPHNNIAPSDEVRLESIKRQILTKLGLSHKPNVSHPLPKQFIWETIYRVDGGRMIPNNAFGSSGKNLDQKTIKLRAFASPGSHLFNGRGGRTDQRSERDPSHHKYRSPFDFTFNISKNNVYGKVLRNRSLERIDKKNSFLNGWTENRQLKINSQIASMPIELKSHHNSSPKELKSGAVRKVNGINGTQMNENALKKSTYPIDINHSIDNKTHTGKNGEMSHNDYEYFNDYSVQTHDKNRYHEGRSSIGYQPAIHNIEYENQKGHHESFADDHENIDHEDFFGNTQEIITFAEEGTQYRQYRILEFSAQNRRVPSQKLSIRSAQIHIRIDKPHSLWIEKAKSLPEKHLLNTKRKWGANKPHHRIKIWVFQLSTSINITEKGIDKAIIFRASFQVDPKNLGWQKFDLTDTIREWYGHTSHEKLRLLIDCTGCGGRYSLHLFQTSKLRGNSSDYLSTNPNRPFLVLHTESSRTRRVRRRAVDCGGALNGQCCKESFYVSFKALGWDDWIIAPRGYFANYCRGDCTGSFRTPDTFQTFHAHFIEEYRKMGLMNGMRPCCAPIKFSSMSLIYYGDDGIIKRDLPKMVVDECGCP

Target ORF information:

RefSeq Version	NM_143685.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Activin-beta (Actbeta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143685.3

ORF Insert Sequence:

ATGCGATTTG CCTTCGATTC TAATCATTCG CAATCGGGGG CGCCATTCAA AGGCAGCAGG 
TGTTTCTTTA ATTGTCAATG CATCTGCTGT CGCCAAGGAT GCTGCGTTGT GGTTGTAAAG 
TGCTGTTGCT GCTTTAACTT AAACTGCTGC AACAGCCTTG GCTCCCGGAA GTCATTTCCA 
CAACCCGCTG CAATGCGTAA AAAAGTTGCT GACCTCGAAG TCCTTAGAGT ATCAAGGTTT 
GTGGCGGTTA TTTTAGTGCT GGCTCGGTGG GTTACTGCGG TAGCGACACT CCTGACAAGC 
TGCATACTCC TAGACATATT TTCCGTGCCT GGCCAGTCTG GAGTTGCAGA TAGAAGCCAA 
GCCAGCAGTA GGACAGTGCA CGTCTCGGTT CCTACCACAC CTAATGAAAC TCCCAGTAGC 
ACTTCGGAGA CGAAGCTAAA GTTGCTTTAT GGGTATACAT CGTATGACAT AAATAACGAC 
CAACAGGTAA AGTCCAACAA TTTATGTAGA GTGCTTTGTA AAAGTCGCAA TCGTAAACGA 
CAGCGAAGGA GGCGACGCCG ACGCAATCAC AGACGACGCA GGCACAGATA TACTAAGCGA 
CTTCATCATC TAATGCAAGA TAATATGAGC GGCTTTGAGC AAAGACTTAA TTTTAGCGAT 
GCCAAATGCC AGTCTTTGGA GACAAATTAC GGAACTAATT ATGACTTAGT ACAAGGAGGT 
AAACTATTTA GTCAGTCAGA GAGAAGCCTA CTGGTGTCCC CTTTGAGGGA AATTGAAGCA 
CCTTGGCCAG CGATTCATGG TTCAATGCGT AACTGTTCAA AGATTAAACG CAATAGAGCC 
AATCTTATTT GGCTTCTAAT TGGACTCGTC TGGTTTGAAG TCAAACTTAT AAATTGCAAT 
GGGATCAGCA GTAGTAATTA TTATGCTTCG AATTTGGAGA GTCACAAGGG CTGCACCTTG 
TGCCATGAAA GCGGAAAGCC CAACATATAC ACCGATAAAG ATAATCCACA CACAGACTAC 
AACATCTACA ACAAATACCA TTCCAACAAT AATTTCAACA AAAAAACTAA TCAACCCCAT 
AACAACATTG CACCCAGCGA TGAAGTTCGT TTGGAGTCAA TAAAACGGCA AATTTTGACA 
AAGCTTGGTC TTAGCCACAA GCCTAATGTA TCTCATCCTT TACCAAAGCA ATTTATTTGG 
GAAACAATAT ATCGAGTCGA TGGTGGACGA ATGATACCAA ACAATGCATT TGGGAGCAGT 
GGTAAGAATT TAGATCAGAA GACTATCAAG CTGAGGGCGT TTGCCTCGCC CGGATCACAT 
TTGTTTAATG GCCGAGGTGG AAGGACCGAT CAGAGAAGCG AACGGGATCC CAGTCATCAT 
AAATATCGAT CCCCGTTCGA TTTCACTTTC AATATAAGTA AAAATAATGT ATATGGGAAA 
GTATTGAGAA ATCGTTCTTT AGAACGAATC GATAAAAAAA ACTCATTCTT AAACGGATGG 
ACAGAAAACC GTCAATTAAA AATAAACTCT CAAATTGCAT CTATGCCTAT AGAACTAAAG 
AGCCACCATA ACAGTAGTCC TAAGGAGCTT AAAAGCGGAG CGGTCAGAAA AGTTAATGGC 
ATCAACGGCA CACAAATGAA TGAGAATGCC TTAAAAAAAT CTACATATCC TATAGATATA 
AATCATAGTA TTGATAATAA AACCCATACT GGCAAAAATG GTGAGATGAG CCACAATGAT 
TATGAATATT TTAACGATTA CAGTGTTCAG ACTCATGATA AAAACCGGTA CCATGAAGGT 
CGCTCTAGCA TTGGATATCA GCCAGCGATC CACAACATAG AATATGAAAA TCAGAAAGGA 
CATCACGAAT CTTTTGCTGA TGATCATGAA AATATTGATC ATGAAGACTT CTTTGGAAAT 
ACGCAGGAAA TAATTACATT TGCTGAAGAG GGAACCCAAT ATCGACAATA TCGAATACTC 
GAGTTTTCGG CCCAAAACCG CCGTGTTCCC AGCCAAAAAT TATCCATACG AAGCGCTCAG 
ATTCATATAA GGATAGACAA GCCACATAGT CTTTGGATTG AAAAAGCAAA AAGTTTGCCA 
GAAAAACATT TACTAAACAC TAAACGAAAA TGGGGAGCAA ATAAACCACA TCACAGAATA 
AAAATATGGG TATTTCAGTT ATCAACTTCA ATCAATATTA CGGAGAAGGG AATAGATAAG 
GCTATTATCT TCCGAGCTTC TTTTCAAGTT GACCCGAAAA ATTTAGGATG GCAAAAATTC 
GATTTAACCG ATACAATCCG TGAATGGTAC GGCCATACCA GTCACGAAAA ACTGCGTTTG 
TTGATTGATT GTACTGGATG CGGTGGTCGT TACTCTTTGC ACCTCTTTCA AACTTCGAAA 
CTGAGAGGAA ACTCATCCGA CTACTTGAGT ACGAATCCCA ATCGACCATT TCTCGTACTT 
CATACAGAAT CATCCAGAAC AAGGCGTGTT AGGCGACGCG CCGTAGATTG TGGCGGAGCT 
TTAAATGGAC AGTGCTGTAA AGAATCTTTT TATGTTTCTT TCAAAGCACT AGGTTGGGAT 
GACTGGATTA TTGCACCCAG AGGATATTTT GCAAACTATT GCCGAGGCGA TTGCACGGGC 
TCTTTTAGGA CGCCAGACAC TTTCCAAACA TTTCATGCTC ATTTTATAGA GGAGTATCGG 
AAAATGGGAC TTATGAATGG AATGCGACCG TGTTGCGCCC CAATAAAATT CTCTAGTATG 
TCCTTAATAT ATTATGGTGA TGATGGTATT ATTAAACGAG ACTTGCCAAA AATGGTTGTT 
GATGAGTGTG GTTGCCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Actbeta cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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