Brd8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brd8
Entrez Gene ID	43460
Full Name	Bromodomain containing 8
Synonyms	CG14514,Dmel\CG14514,dBrd8
General protein information	Preferred Names Bromodomain containing 8 Names CG14514 gene product from transcript CG14514-RA Brd8-PA CG14514-PA bromodomain containing 8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99A1-99A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143428.4	NP_651685.2	bromodomain containing 8

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Brd8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brd8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06483	NM_143428.4 Latest version!	Drosophila melanogaster bromodomain containing 8 (Brd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brd8 ( NM_143428.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651685.2 Drosophila melanogaster bromodomain containing 8 (Brd8), mRNA.

Price & Availability↑

CloneID	OFa06483
Clone ID Related Accession (Same CDS sequence)	NM_143428.4
Accession Version	NM_143428.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	bromodomain containing 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143428.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCCG GGGTACAAGA GCGCCTGCAA CTGAGCCGCA TGCCGCTGGA CACGTGGTCG 
AAGCGGGAGC AGCTCATCCT GGCGTCGGCG GTCTCCTGCA GCGGCGACCA GAACTGGATC 
ACGGTGAGCC GCACGCTGAA GACGATCTGC GGCAATGGCA GCAGTAACCG ACCGGCGGAC 
TGGTTCTCGC AGAAGAACTG TGCCATGCAG TACGGCAATC TGCTGGAGAG CGTGGAGGCC 
ACCAAGCGCA AGAAGCGCAG CAGCGAAAGC AGTGCCGGCG TCTCCTCGCC GGCCACCGTG 
GAGACGCCCA CAGAATTGCT GCTCCGTCGG CTCACGGAGG AGCGTCAGGC GGAGATCAAG 
GCGCAGATGC GTCAGGATCA GGAGACTTAC CGACGCATCC AGCGGGAGAT TGAGTCGCTG 
CAATCGGACG CGGTGACCGA GCAGGAGCTG CAGGACATGT GGCTGGAGAT CGAGAAGGAG 
CAGGAGGCGC GCCGCATCGA GGAGATGAAG CTGGAGAATC GCATGCGGGA GCGCGAGCAG 
CGCAAGAAGG ACCTGGCCAG CAACTGGCGA AACAGCAGTC TAGCCGTCAA GCGTTCCAAC 
CAAGCTGCCG ACACGACTTC CGTCGATATG GACGTGGAGG ACATAAACAC CAGTGGCAAT 
GGCAAACAGC AGACGGGTCC TTCGCCTCTA CTCACATCTC TCCTGAAATC GCCCACAGGC 
AATGCACCTG CAACGCCCAC CCCAGGAGGA GCTGCACCAG CAGCCGGAGG AACTGGCAGC 
GTGGCCAGGG CTACGGCGCC AACCATCACC TCGCTGCTGA CCAGCGGTCC AAGCTCAGCT 
GTTGCCAATC AGCCAGTCAT CACCATGAAG AGCCCCACAG ATGCGGCGTT TATGTCCCGA 
CCTATTACCG GACCTCCGGC CAGTGAGGCT TCTACTACGC CCACACTCGA ACGGAGTCCC 
TCGCAGGCGG CACCCACACT CTCCATGCTG CTAGAGAAGA ACATGGCTGC GGCGAAGGCC 
AACGAAAGCG GAAGCTTGGT AAAGCCAGAG GGGTCAGGAA ACCAGGAGCA GGGTGTAGAC 
GAAGCCACTT CTTCTGCAGC TGGCACAGCA GACTCCGACG AGGCGGATCC CAACGAAGCG 
CAGCTGCTGG AAGTCTTCAA AAACATTGAT CAAATAATAG ATGACATCGA CATTGATATG 
GCCAGTGTCA TTGACGATGA GATTCTGAAA AATGTGGATG ACGTCGTAGC CTCGCCATCA 
AACGATAAAG CCGAAGTTAT TGACTTTGAG GACAAGCTGG ACGCTTTGAA GCGGGAGCAA 
AGTAAAAGTT CCCCAGCCAG TGATAAACCA AAATCTGTGG AGCTAGTAAT TGGATCCAGC 
GATGACTCCA ACGACAATAT ACCCCTGGCT GCCGTGGCCT CCCAGGAGAG TAAGGATCGT 
GGTCAGTCCG GTGGTGAATC TACTAGGGGC ACCGAGGACG CAGAGTCCGA GCCGTTGACC 
AGCGAGGTGG CGGTGGAGGA GATTGGTGAG ACCATTACCA TTATCAGCAC TTCAAGTGAG 
GATACTGCAG GATCGCCACC CACGAAAATG GAGGAAGATG GGGCCACTGA TTCCCTTCCG 
AAGAAGGAGG ATGCCGCGGA AGACAAATCC GAACAGGAAA GTAGCTCCCA AGGACAAAAT 
AAGGCAGGAG GAAGCACGGA CGAGCCGGAA CTAATAGATC ACCAAGCCAC TAGGGAAGCA 
CAAAGCAAGT CAGGCAAGGA CACAGCGGGA AAGCCAGTAA CAATGCAAGA AAAGACGACC 
ACTCAGTCCG CTCCGGTTCC TGTGGATCTG CACGACACAG ACGAGGAGTC GTCTTCGTTG 
ACGGACAGCA GCAAGCACGA TGACCATCCA AGGAGTGCAA AAGCTAAGCC CCCGGCATTG 
AAGCTGGCTC TGGATGACTC CAAGTCGGAA CTGGAGCTGA GTGGCACACC TCAGCCACCG 
TCGGCGCCAG CTCGAACGCC TTTGCTACGA AAACTGCCGC ATCGGGATCG GTCCGAAAGT 
CCAATGGTGG ACGATGACGC CACAACAGCC AGCGATCATT CCACTGCTAG GTCCACACGT 
CGCCGCTGCT CCTCCACGCC CGTTATCGAC AGCATACCCA ATTCTCCCGC CTCCAGCGAG 
CACACCGACG ATCGGAGAGA AACGCGAGCC GCCTCCAAGA AGCTCTTCCT GTCCATTTAT 
GCCATGTTGC TGGACAGCAA GCATGCGGCT CCCTTTAAGC GTCCCTTCCA CGACGAGCAC 
GCCCAGCGAC ATGCGGATCT ATGCCTACGC CCCATGGATT TCCCCACCAT CAAGCGGAAC 
ATCGACTCGG GATTCATTCG CAGCTTGAGC GAGCTGCACA GGGATGTCCT GCTCATGGCC 
CACAACGTTT TGGTGGCCTA CAAACCACAC ACGGCTCAGC ACAAAACGGC GCGTTTGTTC 
GTGCAGGACT GTCAGGCGAT CAAAGAGTTC TCCCAATTGC CGGACGTGCA GGCAGGGATC 
ACGGCCACGC CGGTGAGCAA CACGGGAAGC TTGCGAGCGG ATAAATCGAG CGGAAGCAAG 
GCCAGGAGCG GCTCCCGAAA GAGCCAAAGG CATCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651685.2
CDS	86..2704
Translation	MSAGVQERLQLSRMPLDTWSKREQLILASAVSCSGDQNWITVSRTLKTICGNGSSNRPADWFSQKNCAMQYGNLLESVEATKRKKRSSESSAGVSSPATVETPTELLLRRLTEERQAEIKAQMRQDQETYRRIQREIESLQSDAVTEQELQDMWLEIEKEQEARRIEEMKLENRMREREQRKKDLASNWRNSSLAVKRSNQAADTTSVDMDVEDINTSGNGKQQTGPSPLLTSLLKSPTGNAPATPTPGGAAPAAGGTGSVARATAPTITSLLTSGPSSAVANQPVITMKSPTDAAFMSRPITGPPASEASTTPTLERSPSQAAPTLSMLLEKNMAAAKANESGSLVKPEGSGNQEQGVDEATSSAAGTADSDEADPNEAQLLEVFKNIDQIIDDIDIDMASVIDDEILKNVDDVVASPSNDKAEVIDFEDKLDALKREQSKSSPASDKPKSVELVIGSSDDSNDNIPLAAVASQESKDRGQSGGESTRGTEDAESEPLTSEVAVEEIGETITIISTSSEDTAGSPPTKMEEDGATDSLPKKEDAAEDKSEQESSSQGQNKAGGSTDEPELIDHQATREAQSKSGKDTAGKPVTMQEKTTTQSAPVPVDLHDTDEESSSLTDSSKHDDHPRSAKAKPPALKLALDDSKSELELSGTPQPPSAPARTPLLRKLPHRDRSESPMVDDDATTASDHSTARSTRRRCSSTPVIDSIPNSPASSEHTDDRRETRAASKKLFLSIYAMLLDSKHAAPFKRPFHDEHAQRHADLCLRPMDFPTIKRNIDSGFIRSLSELHRDVLLMAHNVLVAYKPHTAQHKTARLFVQDCQAIKEFSQLPDVQAGITATPVSNTGSLRADKSSGSKARSGSRKSQRHH

Target ORF information:

RefSeq Version	NM_143428.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bromodomain containing 8 (Brd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143428.4

ORF Insert Sequence:

ATGTCTGCCG GGGTACAAGA GCGCCTGCAA CTGAGCCGCA TGCCGCTGGA CACGTGGTCG 
AAGCGGGAGC AGCTCATCCT GGCGTCGGCG GTCTCCTGCA GCGGCGACCA GAACTGGATC 
ACGGTGAGCC GCACGCTGAA GACGATCTGC GGCAATGGCA GCAGTAACCG ACCGGCGGAC 
TGGTTCTCGC AGAAGAACTG TGCCATGCAG TACGGCAATC TGCTGGAGAG CGTGGAGGCC 
ACCAAGCGCA AGAAGCGCAG CAGCGAAAGC AGTGCCGGCG TCTCCTCGCC GGCCACCGTG 
GAGACGCCCA CAGAATTGCT GCTCCGTCGG CTCACGGAGG AGCGTCAGGC GGAGATCAAG 
GCGCAGATGC GTCAGGATCA GGAGACTTAC CGACGCATCC AGCGGGAGAT TGAGTCGCTG 
CAATCGGACG CGGTGACCGA GCAGGAGCTG CAGGACATGT GGCTGGAGAT CGAGAAGGAG 
CAGGAGGCGC GCCGCATCGA GGAGATGAAG CTGGAGAATC GCATGCGGGA GCGCGAGCAG 
CGCAAGAAGG ACCTGGCCAG CAACTGGCGA AACAGCAGTC TAGCCGTCAA GCGTTCCAAC 
CAAGCTGCCG ACACGACTTC CGTCGATATG GACGTGGAGG ACATAAACAC CAGTGGCAAT 
GGCAAACAGC AGACGGGTCC TTCGCCTCTA CTCACATCTC TCCTGAAATC GCCCACAGGC 
AATGCACCTG CAACGCCCAC CCCAGGAGGA GCTGCACCAG CAGCCGGAGG AACTGGCAGC 
GTGGCCAGGG CTACGGCGCC AACCATCACC TCGCTGCTGA CCAGCGGTCC AAGCTCAGCT 
GTTGCCAATC AGCCAGTCAT CACCATGAAG AGCCCCACAG ATGCGGCGTT TATGTCCCGA 
CCTATTACCG GACCTCCGGC CAGTGAGGCT TCTACTACGC CCACACTCGA ACGGAGTCCC 
TCGCAGGCGG CACCCACACT CTCCATGCTG CTAGAGAAGA ACATGGCTGC GGCGAAGGCC 
AACGAAAGCG GAAGCTTGGT AAAGCCAGAG GGGTCAGGAA ACCAGGAGCA GGGTGTAGAC 
GAAGCCACTT CTTCTGCAGC TGGCACAGCA GACTCCGACG AGGCGGATCC CAACGAAGCG 
CAGCTGCTGG AAGTCTTCAA AAACATTGAT CAAATAATAG ATGACATCGA CATTGATATG 
GCCAGTGTCA TTGACGATGA GATTCTGAAA AATGTGGATG ACGTCGTAGC CTCGCCATCA 
AACGATAAAG CCGAAGTTAT TGACTTTGAG GACAAGCTGG ACGCTTTGAA GCGGGAGCAA 
AGTAAAAGTT CCCCAGCCAG TGATAAACCA AAATCTGTGG AGCTAGTAAT TGGATCCAGC 
GATGACTCCA ACGACAATAT ACCCCTGGCT GCCGTGGCCT CCCAGGAGAG TAAGGATCGT 
GGTCAGTCCG GTGGTGAATC TACTAGGGGC ACCGAGGACG CAGAGTCCGA GCCGTTGACC 
AGCGAGGTGG CGGTGGAGGA GATTGGTGAG ACCATTACCA TTATCAGCAC TTCAAGTGAG 
GATACTGCAG GATCGCCACC CACGAAAATG GAGGAAGATG GGGCCACTGA TTCCCTTCCG 
AAGAAGGAGG ATGCCGCGGA AGACAAATCC GAACAGGAAA GTAGCTCCCA AGGACAAAAT 
AAGGCAGGAG GAAGCACGGA CGAGCCGGAA CTAATAGATC ACCAAGCCAC TAGGGAAGCA 
CAAAGCAAGT CAGGCAAGGA CACAGCGGGA AAGCCAGTAA CAATGCAAGA AAAGACGACC 
ACTCAGTCCG CTCCGGTTCC TGTGGATCTG CACGACACAG ACGAGGAGTC GTCTTCGTTG 
ACGGACAGCA GCAAGCACGA TGACCATCCA AGGAGTGCAA AAGCTAAGCC CCCGGCATTG 
AAGCTGGCTC TGGATGACTC CAAGTCGGAA CTGGAGCTGA GTGGCACACC TCAGCCACCG 
TCGGCGCCAG CTCGAACGCC TTTGCTACGA AAACTGCCGC ATCGGGATCG GTCCGAAAGT 
CCAATGGTGG ACGATGACGC CACAACAGCC AGCGATCATT CCACTGCTAG GTCCACACGT 
CGCCGCTGCT CCTCCACGCC CGTTATCGAC AGCATACCCA ATTCTCCCGC CTCCAGCGAG 
CACACCGACG ATCGGAGAGA AACGCGAGCC GCCTCCAAGA AGCTCTTCCT GTCCATTTAT 
GCCATGTTGC TGGACAGCAA GCATGCGGCT CCCTTTAAGC GTCCCTTCCA CGACGAGCAC 
GCCCAGCGAC ATGCGGATCT ATGCCTACGC CCCATGGATT TCCCCACCAT CAAGCGGAAC 
ATCGACTCGG GATTCATTCG CAGCTTGAGC GAGCTGCACA GGGATGTCCT GCTCATGGCC 
CACAACGTTT TGGTGGCCTA CAAACCACAC ACGGCTCAGC ACAAAACGGC GCGTTTGTTC 
GTGCAGGACT GTCAGGCGAT CAAAGAGTTC TCCCAATTGC CGGACGTGCA GGCAGGGATC 
ACGGCCACGC CGGTGAGCAA CACGGGAAGC TTGCGAGCGG ATAAATCGAG CGGAAGCAAG 
GCCAGGAGCG GCTCCCGAAA GAGCCAAAGG CATCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Brd8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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