Mettl3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mettl3
Entrez Gene ID	42844
Full Name	Inducer of meiosis 4
Synonyms	CG5933,Dmel\CG5933,IME4,MTA70,Mta70,ime4
General protein information	Preferred Names Inducer of meiosis 4 Names CG5933 gene product from transcript CG5933-RA CG5933-PA Ime4-PA Mta70 homologue inducer of MEiosis 4 homolog inducer of meiosis 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95D8-95D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_142947.4	NP_651204.1	methyltransferase like 3

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002895	XP_312015.5
Homo sapiens (human)	METTL3	NP_062826.2
Bos taurus (cattle)	METTL3	NP_001095708.1
Drosophila melanogaster (fruit fly)	Ime4	NP_651204.1
Macaca mulatta (Rhesus monkey)	METTL3	XP_001097947.2
Xenopus tropicalis (tropical clawed frog)	mettl3	NP_001016419.1
Pan troglodytes (chimpanzee)	METTL3	NP_001233484.1
Canis lupus familiaris (dog)	METTL3	XP_532627.2
Rattus norvegicus (Norway rat)	Mettl3	NP_001019965.1
Mus musculus (house mouse)	Mettl3	NP_062695.2
Danio rerio (zebrafish)	mettl3	NP_997945.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mettl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mettl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05878	NM_142947.4 Latest version!	Drosophila melanogaster methyltransferase like 3 (Mettl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mettl3 ( NM_142947.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651204.1 Drosophila melanogaster methyltransferase like 3 (Mettl3), mRNA.

Price & Availability↑

CloneID	OFa05878
Clone ID Related Accession (Same CDS sequence)	NM_142947.4
Accession Version	NM_142947.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	methyltransferase like 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142947.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG CGTGGGACAT AAAATCACTC AAGACAAAGC GGAACACGCT CCGCGAGAAG 
CTGGAAAAGC GCAAGAAGGA GCGCATTGAG ATTCTCTCGG ACATTCAGGA GGATCTGACG 
AATCCCAAAA AGGAACTCGT TGAGGCTGAC TTGGAAGTAC AGAAGGAGGT GCTGCAGGCC 
CTCAGTTCCT GCTCCCTGGC CCTGCCCATT GTCTCCACAC AGGTGGTGGA AAAGATTGCC 
GGCAGCAGCC TGGAGATGGT GAACTTTATT CTAGGCAAAC TGGCCAACCA GGGTGCAATT 
GTGATCCGGA ACGTGACCAT CGGCACCGAG GCCGGCTGCG AAATCATCTC TGTGCAGCCC 
AAGGAGCTGA AGGAGATCCT GGAAGACACC AACGATACGT GTCAGCAAAA GGAGGAGGAG 
GCCAAGAGAA AGTTGGAAGT CGACGATGTT GATCAGCCAC AGGAGAAGAC AATAAAACTG 
GAGTCCACTG TAGCGCGGAA AGAGTCCACA AGCCTGGATG CTCCGGACGA TATTATGATG 
CTGCTATCCA TGCCTTCCAC CCGCGAAAAA CAGAGTAAGC AGGTGGGCGA GGAGATTCTT 
GAGCTGCTTA CTAAGCCTAC GGCCAAAGAG CGATCCGTGG CTGAGAAGTT TAAGTCCCAC 
GGCGGAGCGC AGGTCATGGA GTTTTGCTCC CACGGCACAA AAGTCGAGTG CCTGAAGGCT 
CAACAAGCCA CCGCCGAGAT GGCGGCCAAA AAGAAACAAG AAAGAAGAGA CGAAAAGGAG 
CTTCGCCCGG ATGTTGATGC GGGCGAAAAC GTCACCGGTA AGGTACCCAA GACAGAATCG 
GCAGCCGAGG ATGGAGAAAT CATTGCGGAG GTTATAAACA ATTGCGAAGC CGAGTCGCAG 
GAATCCACCG ATGGCAGCGA TACCTGCAGC AGTGAGACAA CAGACAAGTG CACCAAACTG 
CATTTCAAAA AGATCATCCA GGCTCACACG GACGAATCTC TGGGGGACTG CAGTTTCCTA 
AACACCTGCT TCCACATGGC CACCTGCAAG TACGTGCACT ATGAGGTGGA CACCCTGCCG 
CATATAAACA CAAACAAGCC CACGGATGTG AAAACCAAAT TGAGCCTCAA GCGCAGCGTA 
GACTCCAGTT GCACGCTCTA TCCGCCGCAA TGGATTCAAT GCGACCTACG CTTCTTGGAC 
ATGACGGTCC TGGGAAAGTT CGCTGTGGTG ATGGCCGATC CTCCCTGGGA TATCCACATG 
GAATTGCCCT ACGGCACAAT GTCGGACGAT GAAATGAGAG CACTGGGCGT GCCGGCACTT 
CAGGATGACG GCTTGATCTT TCTGTGGGTC ACTGGACGAG CCATGGAACT TGGCCGCGAC 
TGCCTCAAGT TGTGGGGCTA TGAGCGCGTG GACGAACTCA TCTGGGTAAA GACCAACCAA 
CTGCAGCGAA TTATTCGCAC TGGGCGCACT GGTCATTGGC TAAACCACGG CAAGGAGCAC 
TGTTTGGTGG GCATGAAAGG CAATCCCACA AACCTGAACC GCGGACTCGA TTGCGATGTG 
ATCGTTGCGG AGGTGCGGGC CACCTCTCAC AAGCCGGATG AGATTTATGG TATTATCGAG 
CGTCTAAGCC CGGGTACTCG CAAGATCGAG CTCTTTGGGC GTCCGCACAA CATCCAACCG 
AACTGGATAA CCCTGGGAAA CCAACTGGAT GGCATTCGAC TGGTGGATCC GGAGCTAATT 
ACGCAGTTCC AGAAGCGTTA TCCAGATGGT AACTGCATGT CGCCAGCTTC TGCCAATGCG 
GCGTCGATCA ATGGAATACA AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651204.1
CDS	41..1867
Translation	MADAWDIKSLKTKRNTLREKLEKRKKERIEILSDIQEDLTNPKKELVEADLEVQKEVLQALSSCSLALPIVSTQVVEKIAGSSLEMVNFILGKLANQGAIVIRNVTIGTEAGCEIISVQPKELKEILEDTNDTCQQKEEEAKRKLEVDDVDQPQEKTIKLESTVARKESTSLDAPDDIMMLLSMPSTREKQSKQVGEEILELLTKPTAKERSVAEKFKSHGGAQVMEFCSHGTKVECLKAQQATAEMAAKKKQERRDEKELRPDVDAGENVTGKVPKTESAAEDGEIIAEVINNCEAESQESTDGSDTCSSETTDKCTKLHFKKIIQAHTDESLGDCSFLNTCFHMATCKYVHYEVDTLPHINTNKPTDVKTKLSLKRSVDSSCTLYPPQWIQCDLRFLDMTVLGKFAVVMADPPWDIHMELPYGTMSDDEMRALGVPALQDDGLIFLWVTGRAMELGRDCLKLWGYERVDELIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGMKGNPTNLNRGLDCDVIVAEVRATSHKPDEIYGIIERLSPGTRKIELFGRPHNIQPNWITLGNQLDGIRLVDPELITQFQKRYPDGNCMSPASANAASINGIQK

Target ORF information:

RefSeq Version	NM_142947.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methyltransferase like 3 (Mettl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142947.4

ORF Insert Sequence:

ATGGCAGATG CGTGGGACAT AAAATCACTC AAGACAAAGC GGAACACGCT CCGCGAGAAG 
CTGGAAAAGC GCAAGAAGGA GCGCATTGAG ATTCTCTCGG ACATTCAGGA GGATCTGACG 
AATCCCAAAA AGGAACTCGT TGAGGCTGAC TTGGAAGTAC AGAAGGAGGT GCTGCAGGCC 
CTCAGTTCCT GCTCCCTGGC CCTGCCCATT GTCTCCACAC AGGTGGTGGA AAAGATTGCC 
GGCAGCAGCC TGGAGATGGT GAACTTTATT CTAGGCAAAC TGGCCAACCA GGGTGCAATT 
GTGATCCGGA ACGTGACCAT CGGCACCGAG GCCGGCTGCG AAATCATCTC TGTGCAGCCC 
AAGGAGCTGA AGGAGATCCT GGAAGACACC AACGATACGT GTCAGCAAAA GGAGGAGGAG 
GCCAAGAGAA AGTTGGAAGT CGACGATGTT GATCAGCCAC AGGAGAAGAC AATAAAACTG 
GAGTCCACTG TAGCGCGGAA AGAGTCCACA AGCCTGGATG CTCCGGACGA TATTATGATG 
CTGCTATCCA TGCCTTCCAC CCGCGAAAAA CAGAGTAAGC AGGTGGGCGA GGAGATTCTT 
GAGCTGCTTA CTAAGCCTAC GGCCAAAGAG CGATCCGTGG CTGAGAAGTT TAAGTCCCAC 
GGCGGAGCGC AGGTCATGGA GTTTTGCTCC CACGGCACAA AAGTCGAGTG CCTGAAGGCT 
CAACAAGCCA CCGCCGAGAT GGCGGCCAAA AAGAAACAAG AAAGAAGAGA CGAAAAGGAG 
CTTCGCCCGG ATGTTGATGC GGGCGAAAAC GTCACCGGTA AGGTACCCAA GACAGAATCG 
GCAGCCGAGG ATGGAGAAAT CATTGCGGAG GTTATAAACA ATTGCGAAGC CGAGTCGCAG 
GAATCCACCG ATGGCAGCGA TACCTGCAGC AGTGAGACAA CAGACAAGTG CACCAAACTG 
CATTTCAAAA AGATCATCCA GGCTCACACG GACGAATCTC TGGGGGACTG CAGTTTCCTA 
AACACCTGCT TCCACATGGC CACCTGCAAG TACGTGCACT ATGAGGTGGA CACCCTGCCG 
CATATAAACA CAAACAAGCC CACGGATGTG AAAACCAAAT TGAGCCTCAA GCGCAGCGTA 
GACTCCAGTT GCACGCTCTA TCCGCCGCAA TGGATTCAAT GCGACCTACG CTTCTTGGAC 
ATGACGGTCC TGGGAAAGTT CGCTGTGGTG ATGGCCGATC CTCCCTGGGA TATCCACATG 
GAATTGCCCT ACGGCACAAT GTCGGACGAT GAAATGAGAG CACTGGGCGT GCCGGCACTT 
CAGGATGACG GCTTGATCTT TCTGTGGGTC ACTGGACGAG CCATGGAACT TGGCCGCGAC 
TGCCTCAAGT TGTGGGGCTA TGAGCGCGTG GACGAACTCA TCTGGGTAAA GACCAACCAA 
CTGCAGCGAA TTATTCGCAC TGGGCGCACT GGTCATTGGC TAAACCACGG CAAGGAGCAC 
TGTTTGGTGG GCATGAAAGG CAATCCCACA AACCTGAACC GCGGACTCGA TTGCGATGTG 
ATCGTTGCGG AGGTGCGGGC CACCTCTCAC AAGCCGGATG AGATTTATGG TATTATCGAG 
CGTCTAAGCC CGGGTACTCG CAAGATCGAG CTCTTTGGGC GTCCGCACAA CATCCAACCG 
AACTGGATAA CCCTGGGAAA CCAACTGGAT GGCATTCGAC TGGTGGATCC GGAGCTAATT 
ACGCAGTTCC AGAAGCGTTA TCCAGATGGT AACTGCATGT CGCCAGCTTC TGCCAATGCG 
GCGTCGATCA ATGGAATACA AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mettl3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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