twf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	twf
Entrez Gene ID	41719
Full Name	twinfilin
Synonyms	A6,CG3172,Dmel\CG3172,Twf
General protein information	Preferred Names twinfilin Names CG3172 gene product from transcript CG3172-RA CG3172-PA twf-PA twinfillin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88A9-88A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_142081.4	NP_650338.1	twinfilin

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_07617	XP_003711539.1
Eremothecium gossypii	AGOS_ABR105C	NP_983052.2
Anopheles gambiae (African malaria mosquito)	TWF_ANOGA	XP_310246.2
Neurospora crassa	NCU07512	XP_957984.2
Homo sapiens (human)	TWF1	NP_001229326.1
Bos taurus (cattle)	TWF1	NP_001020491.1
Xenopus tropicalis (tropical clawed frog)	twf1	NP_001011469.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003717	XP_310245.5
Mus musculus (house mouse)	Twf1	NP_032997.3
Rattus norvegicus (Norway rat)	Twf1	NP_001008521.1
Schizosaccharomyces pombe (fission yeast)	twf1	NP_588449.1
Macaca mulatta (Rhesus monkey)	LOC700686	XP_002808023.1
Canis lupus familiaris (dog)	TWF1	XP_003433603.1
Caenorhabditis elegans (roundworm)	F38E9.5	NP_510669.1
Kluyveromyces lactis	KLLA0C15719g	XP_452902.1
Gallus gallus (chicken)	TWF1	NP_001265024.1
Danio rerio (zebrafish)	twf1b	NP_001002304.1
Drosophila melanogaster (fruit fly)	twf	NP_650338.1
Saccharomyces cerevisiae (baker's yeast)	TWF1	NP_011594.3
Pan troglodytes (chimpanzee)	TWF1	XP_001165700.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following twf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04835	NM_142081.4 Latest version!	Drosophila melanogaster twinfilin (twf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twf ( NM_142081.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650338.1 Drosophila melanogaster twinfilin (twf), mRNA.

Price & Availability↑

CloneID	OFa04835
Clone ID Related Accession (Same CDS sequence)	NM_142081.4
Accession Version	NM_142081.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	twinfilin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142081.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCACC AAACGGGTAT CCGAGCCAAC GAGCAGCTGG CCAAGGTGTT CGGCAAAGCG 
AAGAATGGTA AATTTCGCGT GATAAAAGTT TCGATCGAGA ATGAGCAGCT TAGTTGCGGC 
GCCACGGCGG AGACGAAAAA GGATTGGGAG CGCGACTACG ACAAGCTTAT AGGTCCCCTA 
CTGGAAAAGG ATGTGCCCTG CTATATTCTC TATCGCCTGG ACGCCAAGAT CCCGCTGGGC 
TACAGTTGGC TACTTATCAG CTGGACGCCG GACACGGCCA GCATCCGACA GAAGATGGTA 
TACGCTTCCA CGAAGGCCAC GTTGAAAACG GAGTTCGGAT CAGCATACAT CACAGAGGAG 
CTGCATGCCA CCACTCTGGA TGAGTGCACG CTGGAGGGCT ATCGCCGGCA CAAGCAGGAT 
TTCGCAGCCC CAGCTCCATT GACCAGCAGG GAGGAGGAGC TGAAAGAGCT GCGAAAGACA 
GAGGTTCACA CTGAGATCAA TACGAATACG CGGCACCAAA CTCTGGGTGG GATCAATTGC 
CCGCTGAGCG AGGCAACCGT GGCTGCCGTT CAGGATTTGG TGCGCGGCAA GCACGACTAC 
CTTCAGTTCC GCATCGATCT GGAGGAGGAA CAGATCCATG TTTCGCGTGC GGCCAAAGTG 
GAGCTCGCCG ATCTGCCGAA GCAAGTGCCC GAGGATCATG CACGATACCA CCTCTTCCTG 
TTTCGGCACA CGCACGAGGG CGACTACTTT GAGTCGTACG TGTTCGTCTA CTCCATGCCT 
GGATATTCCT GCTCCGTGCG CGAGCGCATG ATGTACTCAA GCTGTAAGGC TCCTTTTCTC 
GACGAGCTCG CGGCCCTTGG CGTGGAGGTT GTTAAGAAGC TTGAAATCGA CAGCGGCAGC 
GAGCTGACCG AAGCCTTCTT GCAGGACGAG CTGCACCCAA AGAAGATTCT ACACCGACCG 
GCTTTTGCCA AGCCCAAGGG GCCACCAAAC CGAGGCGCCA AGCGCCTCAC GCGTCCCACC 
GCAGAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650338.1
CDS	59..1090
Translation	MSHQTGIRANEQLAKVFGKAKNGKFRVIKVSIENEQLSCGATAETKKDWERDYDKLIGPLLEKDVPCYILYRLDAKIPLGYSWLLISWTPDTASIRQKMVYASTKATLKTEFGSAYITEELHATTLDECTLEGYRRHKQDFAAPAPLTSREEELKELRKTEVHTEINTNTRHQTLGGINCPLSEATVAAVQDLVRGKHDYLQFRIDLEEEQIHVSRAAKVELADLPKQVPEDHARYHLFLFRHTHEGDYFESYVFVYSMPGYSCSVRERMMYSSCKAPFLDELAALGVEVVKKLEIDSGSELTEAFLQDELHPKKILHRPAFAKPKGPPNRGAKRLTRPTAED

Target ORF information:

RefSeq Version	NM_142081.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twinfilin (twf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142081.4

ORF Insert Sequence:

ATGTCTCACC AAACGGGTAT CCGAGCCAAC GAGCAGCTGG CCAAGGTGTT CGGCAAAGCG 
AAGAATGGTA AATTTCGCGT GATAAAAGTT TCGATCGAGA ATGAGCAGCT TAGTTGCGGC 
GCCACGGCGG AGACGAAAAA GGATTGGGAG CGCGACTACG ACAAGCTTAT AGGTCCCCTA 
CTGGAAAAGG ATGTGCCCTG CTATATTCTC TATCGCCTGG ACGCCAAGAT CCCGCTGGGC 
TACAGTTGGC TACTTATCAG CTGGACGCCG GACACGGCCA GCATCCGACA GAAGATGGTA 
TACGCTTCCA CGAAGGCCAC GTTGAAAACG GAGTTCGGAT CAGCATACAT CACAGAGGAG 
CTGCATGCCA CCACTCTGGA TGAGTGCACG CTGGAGGGCT ATCGCCGGCA CAAGCAGGAT 
TTCGCAGCCC CAGCTCCATT GACCAGCAGG GAGGAGGAGC TGAAAGAGCT GCGAAAGACA 
GAGGTTCACA CTGAGATCAA TACGAATACG CGGCACCAAA CTCTGGGTGG GATCAATTGC 
CCGCTGAGCG AGGCAACCGT GGCTGCCGTT CAGGATTTGG TGCGCGGCAA GCACGACTAC 
CTTCAGTTCC GCATCGATCT GGAGGAGGAA CAGATCCATG TTTCGCGTGC GGCCAAAGTG 
GAGCTCGCCG ATCTGCCGAA GCAAGTGCCC GAGGATCATG CACGATACCA CCTCTTCCTG 
TTTCGGCACA CGCACGAGGG CGACTACTTT GAGTCGTACG TGTTCGTCTA CTCCATGCCT 
GGATATTCCT GCTCCGTGCG CGAGCGCATG ATGTACTCAA GCTGTAAGGC TCCTTTTCTC 
GACGAGCTCG CGGCCCTTGG CGTGGAGGTT GTTAAGAAGC TTGAAATCGA CAGCGGCAGC 
GAGCTGACCG AAGCCTTCTT GCAGGACGAG CTGCACCCAA AGAAGATTCT ACACCGACCG 
GCTTTTGCCA AGCCCAAGGG GCCACCAAAC CGAGGCGCCA AGCGCCTCAC GCGTCCCACC 
GCAGAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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twf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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