Cht5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cht5
Entrez Gene ID	41687
Full Name	Chitinase 5
Synonyms	CG9307,DmCHT5,DmCht5,Dmel\CG9307
General protein information	Preferred Names Chitinase 5 Names CG9307 gene product from transcript CG9307-RA CG9307-PA Cht5-PA chitin-binding peritrophin-A chitinase 5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87F13-87F13

mRNA and Protein(s)

mRNA	Protein	Name
NM_142057.3	NP_650314.1	chitinase 5

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Cht5	NP_650314.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002457	XP_001237469.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cht5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cht5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08360	NM_142057.3 Latest version!	Drosophila melanogaster chitinase 5 (Cht5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cht5 ( NM_142057.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650314.1 Drosophila melanogaster chitinase 5 (Cht5), mRNA.

Price & Availability↑

CloneID	OFa08360
Clone ID Related Accession (Same CDS sequence)	NM_142057.3
Accession Version	NM_142057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	chitinase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142057.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTCGAT ATATTCCGCT CCTACTCCTG GGAGTGCTGT GCTCCTGGCC AGCTGCCACT 
GTGGCCAGTG ACCAGGCTTC CCGCATCGTC TGCTACTTTA GCAACTGGGC CGTGTATCGA 
ACTGGAATCG GTCGCTATGG ATTGGAGGAT GTGCCCGCCG ACCTGTGCAC CCACATCATC 
TACTCCTTTA TTGGTGTTAA CGATAAGAGC TGGGATGTGC TGGTGATCGA TCCTGAGTTG 
GATGTGGATC AGGGTGGCTT CAGCAAGTTT ACACAATTGA AGAAATCTAA TCCCAATGTG 
AAGCTCGAGA TAGCAGTGGG TGGCTGGGCG GAAGGTGGCT CCAAGTACTC ACAAATGGTG 
GCTGTTCGCG ATCGCCGGCA AAGTTTCATA CGCAGTGTGG TACGCTTTAT GAAGCAGTAC 
AACTTCGATG GATTCGACTT GGACTGGGAG TATCCGGGAG CCACGGATCG CGGTGGCAAC 
TATGGGGATA AGGACAAGTT CCTTTATTTT GTTGAGGAAC TGCGACGTGC CTTCGATCGC 
GAGGGTCGCG GATGGGAAAT CACCATGGCG GTGCCCGTTG CCAAGTTCCG GCTGAACGAA 
GGTTACCACG TTCCGGAATT GTGCGAAGCG CTGGATGCCA TTCACGCCAT GACCTATGAC 
CTGCGAGGAA ACTGGGCCGG ATTCGCCGAT GTGCACAGTC CACTCTATAA GCGAAAGCAC 
GACCAATACG CCTACGAGAA ACTGAATGTG AACGACGGCC TTGCGCTGTG GGAGGAGATG 
GGCTGCCCGG CCAACAAGCT GGTGGTGGGT GTTCCCTTCT ACGGTCGGAC GTTCACGCTG 
AGCAACTCGA ACAAGAACTA CAACATGGGC ACCTACATCA ATAAGGAGGC AGGCGGCGGA 
GCTCCGGGTC CCTACACGAA TGCCAGCGGC TTTCTGGCCT ACTACGAAAT TTGCACCGAG 
GTGATGGACA AGTCCAAGGG ATGGACGGTA GAGTGGGATG ACGCTGGCAT GGTGCCCTAC 
ACCTACAAGG ACACACAGTG GGTGGGCTAC GAGAACGAGG CATCCATACA GATCAAGATG 
GACTTTATCA AGCAGCGGGG ATACGCTGGT GCCATGACCT GGGCCATAGA TATGGACGAC 
TTCCATGGAA TGTGCGGCAG GAAGAACGGC CTAACGCAAA TCCTGTACGA CAACATGAAG 
AACTATCGGG TTCCGGAACC TACAAGGCAG ACAACACCAA GGCCTGAGTG GGCAAAGCCG 
CCAGCAACAC CGCCAAATCC AGATGAGGGC GCAGTGGTGG CACCAACTAC TAGCACTACC 
AAGCGGCCAA AACCAAAACC GAAGCCAACT TCAAGTCCAT TGAGCCCAAC ATCCGCTCCA 
GGTCCTGTTC CAACTGTTGG CAGTAGCACT CCGAAGCCAA CCACAAAGAA GCCCAAAAAG 
CCAAAGAAGA CAACAACCAC CACAACAACC ACTCCTGCTC CGGAAAAGAG CACTGAGGAG 
CCAGAAGAAG TTGTTTACCC AGTGGATCCA GTGGAGCCCA CTGATCCCGA GCAGCCTATG 
GGACCGCAGT TCGATCCCAA CGAGATCGAT TGTACCAACC GTGACTTTGT ACCACATCCC 
AATTGCCGAA AGTACTTCCG CTGTGTCCAT GGCAAGCCCG TTGAATTCGA GTGCAAGGAG 
GGAACGGCCT TCCACACGGT CCTGAATGTC TGCGATTGGA TCGAGAACAG CGACCGTTAC 
TACTGCAGTC GAATGAAGAA CAAGGAGAAG GGCAACGAGG CCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650314.1
CDS	131..1918
Translation	MVRYIPLLLLGVLCSWPAATVASDQASRIVCYFSNWAVYRTGIGRYGLEDVPADLCTHIIYSFIGVNDKSWDVLVIDPELDVDQGGFSKFTQLKKSNPNVKLEIAVGGWAEGGSKYSQMVAVRDRRQSFIRSVVRFMKQYNFDGFDLDWEYPGATDRGGNYGDKDKFLYFVEELRRAFDREGRGWEITMAVPVAKFRLNEGYHVPELCEALDAIHAMTYDLRGNWAGFADVHSPLYKRKHDQYAYEKLNVNDGLALWEEMGCPANKLVVGVPFYGRTFTLSNSNKNYNMGTYINKEAGGGAPGPYTNASGFLAYYEICTEVMDKSKGWTVEWDDAGMVPYTYKDTQWVGYENEASIQIKMDFIKQRGYAGAMTWAIDMDDFHGMCGRKNGLTQILYDNMKNYRVPEPTRQTTPRPEWAKPPATPPNPDEGAVVAPTTSTTKRPKPKPKPTSSPLSPTSAPGPVPTVGSSTPKPTTKKPKKPKKTTTTTTTTPAPEKSTEEPEEVVYPVDPVEPTDPEQPMGPQFDPNEIDCTNRDFVPHPNCRKYFRCVHGKPVEFECKEGTAFHTVLNVCDWIENSDRYYCSRMKNKEKGNEAH

Target ORF information:

RefSeq Version	NM_142057.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitinase 5 (Cht5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142057.3

ORF Insert Sequence:

ATGGTTCGAT ATATTCCGCT CCTACTCCTG GGAGTGCTGT GCTCCTGGCC AGCTGCCACT 
GTGGCCAGTG ACCAGGCTTC CCGCATCGTC TGCTACTTTA GCAACTGGGC CGTGTATCGA 
ACTGGAATCG GTCGCTATGG ATTGGAGGAT GTGCCCGCCG ACCTGTGCAC CCACATCATC 
TACTCCTTTA TTGGTGTTAA CGATAAGAGC TGGGATGTGC TGGTGATCGA TCCTGAGTTG 
GATGTGGATC AGGGTGGCTT CAGCAAGTTT ACACAATTGA AGAAATCTAA TCCCAATGTG 
AAGCTCGAGA TAGCAGTGGG TGGCTGGGCG GAAGGTGGCT CCAAGTACTC ACAAATGGTG 
GCTGTTCGCG ATCGCCGGCA AAGTTTCATA CGCAGTGTGG TACGCTTTAT GAAGCAGTAC 
AACTTCGATG GATTCGACTT GGACTGGGAG TATCCGGGAG CCACGGATCG CGGTGGCAAC 
TATGGGGATA AGGACAAGTT CCTTTATTTT GTTGAGGAAC TGCGACGTGC CTTCGATCGC 
GAGGGTCGCG GATGGGAAAT CACCATGGCG GTGCCCGTTG CCAAGTTCCG GCTGAACGAA 
GGTTACCACG TTCCGGAATT GTGCGAAGCG CTGGATGCCA TTCACGCCAT GACCTATGAC 
CTGCGAGGAA ACTGGGCCGG ATTCGCCGAT GTGCACAGTC CACTCTATAA GCGAAAGCAC 
GACCAATACG CCTACGAGAA ACTGAATGTG AACGACGGCC TTGCGCTGTG GGAGGAGATG 
GGCTGCCCGG CCAACAAGCT GGTGGTGGGT GTTCCCTTCT ACGGTCGGAC GTTCACGCTG 
AGCAACTCGA ACAAGAACTA CAACATGGGC ACCTACATCA ATAAGGAGGC AGGCGGCGGA 
GCTCCGGGTC CCTACACGAA TGCCAGCGGC TTTCTGGCCT ACTACGAAAT TTGCACCGAG 
GTGATGGACA AGTCCAAGGG ATGGACGGTA GAGTGGGATG ACGCTGGCAT GGTGCCCTAC 
ACCTACAAGG ACACACAGTG GGTGGGCTAC GAGAACGAGG CATCCATACA GATCAAGATG 
GACTTTATCA AGCAGCGGGG ATACGCTGGT GCCATGACCT GGGCCATAGA TATGGACGAC 
TTCCATGGAA TGTGCGGCAG GAAGAACGGC CTAACGCAAA TCCTGTACGA CAACATGAAG 
AACTATCGGG TTCCGGAACC TACAAGGCAG ACAACACCAA GGCCTGAGTG GGCAAAGCCG 
CCAGCAACAC CGCCAAATCC AGATGAGGGC GCAGTGGTGG CACCAACTAC TAGCACTACC 
AAGCGGCCAA AACCAAAACC GAAGCCAACT TCAAGTCCAT TGAGCCCAAC ATCCGCTCCA 
GGTCCTGTTC CAACTGTTGG CAGTAGCACT CCGAAGCCAA CCACAAAGAA GCCCAAAAAG 
CCAAAGAAGA CAACAACCAC CACAACAACC ACTCCTGCTC CGGAAAAGAG CACTGAGGAG 
CCAGAAGAAG TTGTTTACCC AGTGGATCCA GTGGAGCCCA CTGATCCCGA GCAGCCTATG 
GGACCGCAGT TCGATCCCAA CGAGATCGAT TGTACCAACC GTGACTTTGT ACCACATCCC 
AATTGCCGAA AGTACTTCCG CTGTGTCCAT GGCAAGCCCG TTGAATTCGA GTGCAAGGAG 
GGAACGGCCT TCCACACGGT CCTGAATGTC TGCGATTGGA TCGAGAACAG CGACCGTTAC 
TACTGCAGTC GAATGAAGAA CAAGGAGAAG GGCAACGAGG CCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cht5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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