Tpc1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpc1
Entrez Gene ID	41316
Full Name	Thiamine pyrophosphate carrier protein 1
Synonyms	CG6608,DmTpc1,Dmel\CG6608,Q9VGU9
General protein information	Preferred Names Thiamine pyrophosphate carrier protein 1 Names CG6608 gene product from transcript CG6608-RA CG6608-PA CG6608-PB Tpc1-PA Tpc1-PB thiamine pyrophosphate carrier protein thiamine pyrophosphate carrier protein 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86C7-86C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_141777.4	NP_650034.1	thiamine pyrophosphate carrier protein 1, isoform A
NM_169372.3	NP_731527.1	thiamine pyrophosphate carrier protein 1, isoform B

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TPC1	NP_011610.3
Gallus gallus (chicken)	SLC25A19	XP_004946425.1
Caenorhabditis elegans (roundworm)	hpo-12	NP_001255632.1
Homo sapiens (human)	SLC25A19	NP_001119593.1
Canis lupus familiaris (dog)	SLC25A19	XP_005624165.1
Drosophila melanogaster (fruit fly)	Tpc1	NP_650034.1
Neurospora crassa	NCU07384	XP_958877.1
Eremothecium gossypii	AGOS_AAR036W	NP_982577.1
Bos taurus (cattle)	SLC25A19	NP_001039352.1
Rattus norvegicus (Norway rat)	Slc25a19	NP_001007675.1
Danio rerio (zebrafish)	slc25a19	NP_991278.1
Arabidopsis thaliana (thale cress)	AT3G21390	NP_566683.1
Oryza sativa (rice)	Os01g0934200	NP_001045309.1
Schizosaccharomyces pombe (fission yeast)	SPBC1604.04	NP_596636.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006806	XP_308941.4
Kluyveromyces lactis	KLLA0D15015g	XP_453726.1
Pan troglodytes (chimpanzee)	SLC25A19	NP_001233547.1
Macaca mulatta (Rhesus monkey)	SLC25A19	XP_001094462.2
Mus musculus (house mouse)	Slc25a19	NP_080347.1
Arabidopsis thaliana (thale cress)	AT5G48970	NP_199708.1
Xenopus tropicalis (tropical clawed frog)	slc25a19	NP_001015953.1
Magnaporthe oryzae (rice blast fungus)	MGG_00489	XP_003718573.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005113	XP_313991.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tpc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08320	NM_169372.3 Latest version!	Drosophila melanogaster thiamine pyrophosphate carrier protein 1, transcript variant B (Tpc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa08320	NM_141777.4 Latest version!	Drosophila melanogaster thiamine pyrophosphate carrier protein 1, transcript variant A (Tpc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpc1 ( NM_141777.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650034.1 Drosophila melanogaster thiamine pyrophosphate carrier protein 1, transcript variant A (Tpc1), mRNA.

Price & Availability↑

CloneID	OFa08320
Clone ID Related Accession (Same CDS sequence)	NM_141777.4 , NM_169372.3
Accession Version	NM_141777.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	thiamine pyrophosphate carrier protein 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141777.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGTAA CCACAGGCTC CACCAGTGAG GCGACCACAA CCACAACTCC CGTTCCGCGA 
CGCAAGCACA GTACGCGGGA GCAGCTGCAT CAAATGCTGG CCGGTGGACT TTCGGCGGCC 
ATTACGCGCT CCACCTGCCA GCCGCTGGAC GTGCTAAAGA TCCGCTTCCA GCTGCAGGTG 
GAGCCGCTGG GCAAGAACGC CGCCAAGGAG GGACCAGGAG CCCTTACCTC CAAATACACA 
TCCATCGGAC AGGCGGTGAA AACCATATAC CGCGAGGAGG GAATGTTGGC CTTCTGGAAG 
GGACACAATC CTGCCCAAGT GCTGAGCATA ATGTACGGCA TTTGTCAGTT CTGGACGTAC 
GAGCAGCTCA GCCTGATGGC CAAACAGACC AGCTATCTGG CGGATCATCA GCATCTATCG 
AACTTCCTGT GCGGCGCTGC GGCCGGAGGA GCAGCAGTGA TCATATCCAC GCCGCTGGAC 
GTCATCCGCA CTCGTCTGAT TGCCCAGGAC ACCAGCAAGG GATACAGGAA TGCAACAAGG 
GCTGTGTCGG CGATTGTCCG CCAGGAGGGA CCTCGCGGCA TGTACCGCGG TCTGTCCTCC 
GCCCTGCTGC AAATAACGCC GCTGATGGGC ACCAACTTTA TGGCTTACCG CCTGTTCAGC 
GACTGGGCGT GTGCCTTCTT GGAGGTGAGC GATCGCAGCC AATTGCCCAC GTGGACGCTG 
CTGGGATTGG GCGCCTCCTC GGGCATGCTA TCCAAGACGA TCGTGTATCC CTTCGATTTG 
ATCAAAAAGC GACTGCAAAT CCAGGGCTTC GAGTCGAATC GCCAGACCTT CGGACAGACA 
CTGCAGTGCC ACGGAGTCTG GGACTGTTTG CGGCTGACCG TGCGCCAAGA GGGCGTTAGG 
GGCTTGTACA AGGGCGTGGC CCCCACACTC CTTAAGTCAA GCATGACGAC GGCCCTGTAC 
TTCAGCATTT ACGATAAGCT CAAGCAGGTG CGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650034.1
CDS	630..1628
Translation	MAVTTGSTSEATTTTTPVPRRKHSTREQLHQMLAGGLSAAITRSTCQPLDVLKIRFQLQVEPLGKNAAKEGPGALTSKYTSIGQAVKTIYREEGMLAFWKGHNPAQVLSIMYGICQFWTYEQLSLMAKQTSYLADHQHLSNFLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKGYRNATRAVSAIVRQEGPRGMYRGLSSALLQITPLMGTNFMAYRLFSDWACAFLEVSDRSQLPTWTLLGLGASSGMLSKTIVYPFDLIKKRLQIQGFESNRQTFGQTLQCHGVWDCLRLTVRQEGVRGLYKGVAPTLLKSSMTTALYFSIYDKLKQVRF

Target ORF information:

RefSeq Version	NM_141777.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thiamine pyrophosphate carrier protein 1, transcript variant A (Tpc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141777.4

ORF Insert Sequence:

ATGGCGGTAA CCACAGGCTC CACCAGTGAG GCGACCACAA CCACAACTCC CGTTCCGCGA 
CGCAAGCACA GTACGCGGGA GCAGCTGCAT CAAATGCTGG CCGGTGGACT TTCGGCGGCC 
ATTACGCGCT CCACCTGCCA GCCGCTGGAC GTGCTAAAGA TCCGCTTCCA GCTGCAGGTG 
GAGCCGCTGG GCAAGAACGC CGCCAAGGAG GGACCAGGAG CCCTTACCTC CAAATACACA 
TCCATCGGAC AGGCGGTGAA AACCATATAC CGCGAGGAGG GAATGTTGGC CTTCTGGAAG 
GGACACAATC CTGCCCAAGT GCTGAGCATA ATGTACGGCA TTTGTCAGTT CTGGACGTAC 
GAGCAGCTCA GCCTGATGGC CAAACAGACC AGCTATCTGG CGGATCATCA GCATCTATCG 
AACTTCCTGT GCGGCGCTGC GGCCGGAGGA GCAGCAGTGA TCATATCCAC GCCGCTGGAC 
GTCATCCGCA CTCGTCTGAT TGCCCAGGAC ACCAGCAAGG GATACAGGAA TGCAACAAGG 
GCTGTGTCGG CGATTGTCCG CCAGGAGGGA CCTCGCGGCA TGTACCGCGG TCTGTCCTCC 
GCCCTGCTGC AAATAACGCC GCTGATGGGC ACCAACTTTA TGGCTTACCG CCTGTTCAGC 
GACTGGGCGT GTGCCTTCTT GGAGGTGAGC GATCGCAGCC AATTGCCCAC GTGGACGCTG 
CTGGGATTGG GCGCCTCCTC GGGCATGCTA TCCAAGACGA TCGTGTATCC CTTCGATTTG 
ATCAAAAAGC GACTGCAAAT CCAGGGCTTC GAGTCGAATC GCCAGACCTT CGGACAGACA 
CTGCAGTGCC ACGGAGTCTG GGACTGTTTG CGGCTGACCG TGCGCCAAGA GGGCGTTAGG 
GGCTTGTACA AGGGCGTGGC CCCCACACTC CTTAAGTCAA GCATGACGAC GGCCCTGTAC 
TTCAGCATTT ACGATAAGCT CAAGCAGGTG CGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpc1 ( NM_169372.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731527.1 Drosophila melanogaster thiamine pyrophosphate carrier protein 1, transcript variant B (Tpc1), mRNA.

Price & Availability↑

CloneID	OFa08320
Clone ID Related Accession (Same CDS sequence)	NM_141777.4 , NM_169372.3
Accession Version	NM_169372.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	thiamine pyrophosphate carrier protein 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169372.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGTAA CCACAGGCTC CACCAGTGAG GCGACCACAA CCACAACTCC CGTTCCGCGA 
CGCAAGCACA GTACGCGGGA GCAGCTGCAT CAAATGCTGG CCGGTGGACT TTCGGCGGCC 
ATTACGCGCT CCACCTGCCA GCCGCTGGAC GTGCTAAAGA TCCGCTTCCA GCTGCAGGTG 
GAGCCGCTGG GCAAGAACGC CGCCAAGGAG GGACCAGGAG CCCTTACCTC CAAATACACA 
TCCATCGGAC AGGCGGTGAA AACCATATAC CGCGAGGAGG GAATGTTGGC CTTCTGGAAG 
GGACACAATC CTGCCCAAGT GCTGAGCATA ATGTACGGCA TTTGTCAGTT CTGGACGTAC 
GAGCAGCTCA GCCTGATGGC CAAACAGACC AGCTATCTGG CGGATCATCA GCATCTATCG 
AACTTCCTGT GCGGCGCTGC GGCCGGAGGA GCAGCAGTGA TCATATCCAC GCCGCTGGAC 
GTCATCCGCA CTCGTCTGAT TGCCCAGGAC ACCAGCAAGG GATACAGGAA TGCAACAAGG 
GCTGTGTCGG CGATTGTCCG CCAGGAGGGA CCTCGCGGCA TGTACCGCGG TCTGTCCTCC 
GCCCTGCTGC AAATAACGCC GCTGATGGGC ACCAACTTTA TGGCTTACCG CCTGTTCAGC 
GACTGGGCGT GTGCCTTCTT GGAGGTGAGC GATCGCAGCC AATTGCCCAC GTGGACGCTG 
CTGGGATTGG GCGCCTCCTC GGGCATGCTA TCCAAGACGA TCGTGTATCC CTTCGATTTG 
ATCAAAAAGC GACTGCAAAT CCAGGGCTTC GAGTCGAATC GCCAGACCTT CGGACAGACA 
CTGCAGTGCC ACGGAGTCTG GGACTGTTTG CGGCTGACCG TGCGCCAAGA GGGCGTTAGG 
GGCTTGTACA AGGGCGTGGC CCCCACACTC CTTAAGTCAA GCATGACGAC GGCCCTGTAC 
TTCAGCATTT ACGATAAGCT CAAGCAGGTG CGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731527.1
CDS	729..1727
Translation	MAVTTGSTSEATTTTTPVPRRKHSTREQLHQMLAGGLSAAITRSTCQPLDVLKIRFQLQVEPLGKNAAKEGPGALTSKYTSIGQAVKTIYREEGMLAFWKGHNPAQVLSIMYGICQFWTYEQLSLMAKQTSYLADHQHLSNFLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKGYRNATRAVSAIVRQEGPRGMYRGLSSALLQITPLMGTNFMAYRLFSDWACAFLEVSDRSQLPTWTLLGLGASSGMLSKTIVYPFDLIKKRLQIQGFESNRQTFGQTLQCHGVWDCLRLTVRQEGVRGLYKGVAPTLLKSSMTTALYFSIYDKLKQVRF

Target ORF information:

RefSeq Version	NM_169372.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thiamine pyrophosphate carrier protein 1, transcript variant B (Tpc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169372.3

ORF Insert Sequence:

ATGGCGGTAA CCACAGGCTC CACCAGTGAG GCGACCACAA CCACAACTCC CGTTCCGCGA 
CGCAAGCACA GTACGCGGGA GCAGCTGCAT CAAATGCTGG CCGGTGGACT TTCGGCGGCC 
ATTACGCGCT CCACCTGCCA GCCGCTGGAC GTGCTAAAGA TCCGCTTCCA GCTGCAGGTG 
GAGCCGCTGG GCAAGAACGC CGCCAAGGAG GGACCAGGAG CCCTTACCTC CAAATACACA 
TCCATCGGAC AGGCGGTGAA AACCATATAC CGCGAGGAGG GAATGTTGGC CTTCTGGAAG 
GGACACAATC CTGCCCAAGT GCTGAGCATA ATGTACGGCA TTTGTCAGTT CTGGACGTAC 
GAGCAGCTCA GCCTGATGGC CAAACAGACC AGCTATCTGG CGGATCATCA GCATCTATCG 
AACTTCCTGT GCGGCGCTGC GGCCGGAGGA GCAGCAGTGA TCATATCCAC GCCGCTGGAC 
GTCATCCGCA CTCGTCTGAT TGCCCAGGAC ACCAGCAAGG GATACAGGAA TGCAACAAGG 
GCTGTGTCGG CGATTGTCCG CCAGGAGGGA CCTCGCGGCA TGTACCGCGG TCTGTCCTCC 
GCCCTGCTGC AAATAACGCC GCTGATGGGC ACCAACTTTA TGGCTTACCG CCTGTTCAGC 
GACTGGGCGT GTGCCTTCTT GGAGGTGAGC GATCGCAGCC AATTGCCCAC GTGGACGCTG 
CTGGGATTGG GCGCCTCCTC GGGCATGCTA TCCAAGACGA TCGTGTATCC CTTCGATTTG 
ATCAAAAAGC GACTGCAAAT CCAGGGCTTC GAGTCGAATC GCCAGACCTT CGGACAGACA 
CTGCAGTGCC ACGGAGTCTG GGACTGTTTG CGGCTGACCG TGCGCCAAGA GGGCGTTAGG 
GGCTTGTACA AGGGCGTGGC CCCCACACTC CTTAAGTCAA GCATGACGAC GGCCCTGTAC 
TTCAGCATTT ACGATAAGCT CAAGCAGGTG CGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tpc1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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