Nmdar1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nmdar1
Entrez Gene ID	40665
Full Name	NMDA receptor 1
Synonyms	CG2902,DMNMDAR1,DNMDAR-I,DNmdar,DmelNmdar1,Dmel\CG2902,DrNR1,NMDA,NMDA R1,NMDAR,NMDAR1,NMdar1,NR1,NRI,Nmdar,dNR1,dnmdar-I,dsNR1,nmr,nr1
General protein information	Preferred Names NMDA receptor 1 Names CG2902 gene product from transcript CG2902-RA CG2902-PA NMDA receptor NMDA receptor I NMDAR Nmdar1-PA dNR1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83A6-83A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_169059.2	NP_730940.1	NMDA receptor 1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001478	XP_321646.3
Caenorhabditis elegans (roundworm)	nmr-1	NP_495033.2
Xenopus tropicalis (tropical clawed frog)	grin1	NP_001072593.1
Danio rerio (zebrafish)	grin1b	NP_001137603.1
Homo sapiens (human)	GRIN1	NP_001172019.1
Bos taurus (cattle)	GRIN1	XP_002691748.3
Mus musculus (house mouse)	Grin1	NP_032195.1
Rattus norvegicus (Norway rat)	Grin1	NP_001257535.1
Gallus gallus (chicken)	GRIN1	NP_996862.1
Macaca mulatta (Rhesus monkey)	GRIN1	XP_001117773.1
Canis lupus familiaris (dog)	GRIN1	NP_001008717.1
Danio rerio (zebrafish)	grin1a	NP_001070182.2
Drosophila melanogaster (fruit fly)	Nmdar1	NP_730940.1
Arabidopsis thaliana (thale cress)	GLR5	NP_565744.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nmdar1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nmdar1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03943	NM_169059.2 Latest version!	Drosophila melanogaster NMDA receptor 1 (Nmdar1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nmdar1 ( NM_169059.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730940.1 Drosophila melanogaster NMDA receptor 1 (Nmdar1), mRNA.

Price & Availability↑

CloneID	OFa03943
Clone ID Related Accession (Same CDS sequence)	NM_169059.2
Accession Version	NM_169059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	NMDA receptor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169059.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTATGG CTGAGTTTGT TTTTTGCCGG CCATTGTTTG GGCTCGCCAT TGTTCTCCTG 
GTGGCGCCAA TTGATGCCGC CCAGAGGCAC ACTGCCTCGG ATAATCCCTC GACGTACAAC 
ATTGGCGGCG TACTGAGCAA TTCCGACAGC GAGGAGCACT TTAGCACCAC AATAAAGCAC 
CTCAACTTCG ATCAGCAGTA TGTGCCGCGG AAGGTCACCT ACTACGACAA AACCATCCGC 
ATGGACAAGA ATCCCATCAA GACGGTCTTC AATGTGTGCG ACAAGCTCAT CGAGAATCGG 
GTGTACGCCG TAGTCGTTTC GCATGAGCAA ACCTCAGGTG ATTTGTCGCC GGCGGCCGTG 
AGTTATACAA GCGGATTCTA TTCAATTCCC GTCATTGGCA TCTCCTCGCG GGACGCGGCC 
TTCTCCGACA AGAACATCCA CGTCTCCTTC CTGCGGACGG TGCCGCCGTA CTACCACCAG 
GCGGATGTCT GGCTGGAGAT GCTCAGCCAC TTTGCCTACA CGAAGGTAAT CATCATCCAC 
AGCTCCGACA CGGATGGCCG GGCCATCCTG GGTCGCTTCC AGACGACATC GCAGACTTAC 
TACGACGACG TGGATGTGCG CGCCACCGTG GAGCTGATTG TCGAGTTCGA GCCCAAGCTG 
GAGAGCTTCA CCGAGCACCT CATCGACATG AAGACCGCCC AGTCCAGGGT CTACCTCATG 
TACGCGAGCA CGGAAGACGC TCAAGTAATC TTCCGCGATG CCGGGGAGTA CAACATGACC 
GGTGAGGGTC ACGTGTGGAT TGTAACGGAG CAGGCGCTGT TCTCCAACAA CACTCCGGAC 
GGAGTACTGG GTCTCCAGCT GGAGCACGCC CATAGTGACA AGGGGCACAT CAGGGACAGC 
GTTTATGTTT TGGCGTCGGC CATCAAGGAG ATGATTTCCA ACGAGACCAT TGCCGAGGCT 
CCCAAGGATT GCGGCGATTC GGCCGTTAAC TGGGAATCGG GCAAGCGACT GTTTCAGTAC 
CTCAAAAGCC GTAATATCAC CGGCGAAACG GGTCAGGTGG CCTTCGATGA CAATGGTGAC 
CGAATCTACG CCGGCTACGA TGTGATCAAC ATTCGGGAGC AGCAGAAGAA GCACGTGGTT 
GGAAAGTTCA GCTACGACAG CATGCGGGCC AAAATGCGGA TGAGGATCAA CGACAGCGAG 
ATCATTTGGC CGGGAAAGCA GCGCCGCAAG CCCGAGGGCA TCATGATTCC CACCCACCTG 
AGGCTGCTCA CCATCGAGGA GAAGCCCTTC GTCTACGTGC GGCGCATGGG CGACGATGAG 
TTCCGCTGTG AGCCGGATGA GCGTCCGTGT CCGCTGTTCA ACAACTCGGA TGCCACGGCC 
AACGAGTTCT GCTGCCGCGG CTACTGCATC GACCTGCTGA TCGAACTGTC CAAACGGATC 
AACTTCACCT ACGACCTGGC CCTCTCGCCG GATGGTCAGT TTGGGCACTA CATCCTTCGG 
AACAACACGG GAGCGATGAC GTTGCGCAAG GAGTGGACGG GCCTCATCGG GGAGCTGGTC 
AACGAACGTG CCGACATGAT CGTGGCACCA CTAACCATCA ATCCGGAGCG TGCCGAGTAC 
ATTGAATTCT CGAAGCCATT CAAATACCAA GGCATTACAA TACTCGAGAA GAAACCGTCA 
CGATCGAGTA CTCTCGTGTC TTTCTTGCAG CCGTTTAGTA ACACGCTATG GATCTTGGTA 
ATGGTGTCGG TCCATGTTGT GGCGCTCGTG CTCTACCTGC TGGACAGATT CTCGCCGTTC 
GGACGCTTCA AGCTCTCGCA CTCGGACAGC AACGAGGAGA AGGCCCTCAA TCTCAGCTCC 
GCGGTGTGGT TCGCCTGGGG AGTCCTGCTG AACAGCGGGA TCGGCGAGGG CACGCCCCGG 
AGCTTCTCCG CCCGGGTGCT GGGCATGGTC TGGGCCGGAT TTGCTATGAT CATCGTTGCC 
TCGTACACCG CCAACCTGGC CGCCTTCCTC GTGCTGGAGC GGCCCAAGAC CAAGTTGAGC 
GGCATCAACG ACGCCCGCTT GCGGAACACC ATGGAGAACC TGACCTGCGC CACCGTCAAG 
GGCTCCTCCG TGGACATGTA CTTCCGTCGC CAGGTGGAGC TCTCCAACAT GTATCGCACC 
ATGGAGGCGA ACAACTACGC CACCGCCGAG CAGGCCATCC AGGATGTGAA GAAGGGCAAG 
CTTATGGCCT TCATCTGGGA TTCATCTCGG CTGGAGTACG AGGCCTCCAA GGATTGCGAA 
CTAGTTACAG CCGGAGAGCT GTTCGGACGA AGTGGGTACG GGATTGGACT GCAAAAGGGC 
TCGCCCTGGA CGGATGCGGT CACGCTGGCC ATCTTGGAGT TCCACGAAAG CGGTTTCATG 
GAAAAATTGG ACAAACAGTG GATCTTTCAC GGACACGTCC AGCAGAACTG CGAGCTGTTC 
GAGAAGACGC CCAACACTCT TGGGCTTAAA AACATGGCGG GAGTGTTCAT ACTGGTGGGT 
GTGGGCATTG CCGGCGGCGT GGGCCTGATC ATCATCGAGG TCATCTACAA GAAGCACCAG 
GTGAAGAAGC AGAAGCGCCT GGACATTGCC CGACATGCGG CGGACAAGTG GCGGGGCACC 
ATAGAGAAAC GAAAGACTAT TCGTGCCTCG CTGGCGATGC AGCGACAGTA CAACGTGGGG 
CTGAACTCCA CCCATGCCCC GGGCACCATC AGTCTGGCAG TGGACAAGCG CCGGTATCCT 
CGCCTCGGTC AGCGACTGGG ACCGGAGAGA GCCTGGCCAG GAGACGCCGC CGACGTTCTG 
CGCATCCGTC GGCCCTACGA ACTGGGCAAT CCTGGCCAGA GTCCGAAGGT GATGGCTGCC 
AACCAGCCGG GAATGCCCAT GCCCATGCTG GGCAAGACAC GGCCCCAACA GAGTGTCCTG 
CCACCTCGCT ACTCGCCCGG ATACACCAGC GACGTGTCGC ATCTGGTCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730940.1
CDS	241..3234
Translation	MAMAEFVFCRPLFGLAIVLLVAPIDAAQRHTASDNPSTYNIGGVLSNSDSEEHFSTTIKHLNFDQQYVPRKVTYYDKTIRMDKNPIKTVFNVCDKLIENRVYAVVVSHEQTSGDLSPAAVSYTSGFYSIPVIGISSRDAAFSDKNIHVSFLRTVPPYYHQADVWLEMLSHFAYTKVIIIHSSDTDGRAILGRFQTTSQTYYDDVDVRATVELIVEFEPKLESFTEHLIDMKTAQSRVYLMYASTEDAQVIFRDAGEYNMTGEGHVWIVTEQALFSNNTPDGVLGLQLEHAHSDKGHIRDSVYVLASAIKEMISNETIAEAPKDCGDSAVNWESGKRLFQYLKSRNITGETGQVAFDDNGDRIYAGYDVINIREQQKKHVVGKFSYDSMRAKMRMRINDSEIIWPGKQRRKPEGIMIPTHLRLLTIEEKPFVYVRRMGDDEFRCEPDERPCPLFNNSDATANEFCCRGYCIDLLIELSKRINFTYDLALSPDGQFGHYILRNNTGAMTLRKEWTGLIGELVNERADMIVAPLTINPERAEYIEFSKPFKYQGITILEKKPSRSSTLVSFLQPFSNTLWILVMVSVHVVALVLYLLDRFSPFGRFKLSHSDSNEEKALNLSSAVWFAWGVLLNSGIGEGTPRSFSARVLGMVWAGFAMIIVASYTANLAAFLVLERPKTKLSGINDARLRNTMENLTCATVKGSSVDMYFRRQVELSNMYRTMEANNYATAEQAIQDVKKGKLMAFIWDSSRLEYEASKDCELVTAGELFGRSGYGIGLQKGSPWTDAVTLAILEFHESGFMEKLDKQWIFHGHVQQNCELFEKTPNTLGLKNMAGVFILVGVGIAGGVGLIIIEVIYKKHQVKKQKRLDIARHAADKWRGTIEKRKTIRASLAMQRQYNVGLNSTHAPGTISLAVDKRRYPRLGQRLGPERAWPGDAADVLRIRRPYELGNPGQSPKVMAANQPGMPMPMLGKTRPQQSVLPPRYSPGYTSDVSHLVV

Target ORF information:

RefSeq Version	NM_169059.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NMDA receptor 1 (Nmdar1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169059.2

ORF Insert Sequence:

ATGGCTATGG CTGAGTTTGT TTTTTGCCGG CCATTGTTTG GGCTCGCCAT TGTTCTCCTG 
GTGGCGCCAA TTGATGCCGC CCAGAGGCAC ACTGCCTCGG ATAATCCCTC GACGTACAAC 
ATTGGCGGCG TACTGAGCAA TTCCGACAGC GAGGAGCACT TTAGCACCAC AATAAAGCAC 
CTCAACTTCG ATCAGCAGTA TGTGCCGCGG AAGGTCACCT ACTACGACAA AACCATCCGC 
ATGGACAAGA ATCCCATCAA GACGGTCTTC AATGTGTGCG ACAAGCTCAT CGAGAATCGG 
GTGTACGCCG TAGTCGTTTC GCATGAGCAA ACCTCAGGTG ATTTGTCGCC GGCGGCCGTG 
AGTTATACAA GCGGATTCTA TTCAATTCCC GTCATTGGCA TCTCCTCGCG GGACGCGGCC 
TTCTCCGACA AGAACATCCA CGTCTCCTTC CTGCGGACGG TGCCGCCGTA CTACCACCAG 
GCGGATGTCT GGCTGGAGAT GCTCAGCCAC TTTGCCTACA CGAAGGTAAT CATCATCCAC 
AGCTCCGACA CGGATGGCCG GGCCATCCTG GGTCGCTTCC AGACGACATC GCAGACTTAC 
TACGACGACG TGGATGTGCG CGCCACCGTG GAGCTGATTG TCGAGTTCGA GCCCAAGCTG 
GAGAGCTTCA CCGAGCACCT CATCGACATG AAGACCGCCC AGTCCAGGGT CTACCTCATG 
TACGCGAGCA CGGAAGACGC TCAAGTAATC TTCCGCGATG CCGGGGAGTA CAACATGACC 
GGTGAGGGTC ACGTGTGGAT TGTAACGGAG CAGGCGCTGT TCTCCAACAA CACTCCGGAC 
GGAGTACTGG GTCTCCAGCT GGAGCACGCC CATAGTGACA AGGGGCACAT CAGGGACAGC 
GTTTATGTTT TGGCGTCGGC CATCAAGGAG ATGATTTCCA ACGAGACCAT TGCCGAGGCT 
CCCAAGGATT GCGGCGATTC GGCCGTTAAC TGGGAATCGG GCAAGCGACT GTTTCAGTAC 
CTCAAAAGCC GTAATATCAC CGGCGAAACG GGTCAGGTGG CCTTCGATGA CAATGGTGAC 
CGAATCTACG CCGGCTACGA TGTGATCAAC ATTCGGGAGC AGCAGAAGAA GCACGTGGTT 
GGAAAGTTCA GCTACGACAG CATGCGGGCC AAAATGCGGA TGAGGATCAA CGACAGCGAG 
ATCATTTGGC CGGGAAAGCA GCGCCGCAAG CCCGAGGGCA TCATGATTCC CACCCACCTG 
AGGCTGCTCA CCATCGAGGA GAAGCCCTTC GTCTACGTGC GGCGCATGGG CGACGATGAG 
TTCCGCTGTG AGCCGGATGA GCGTCCGTGT CCGCTGTTCA ACAACTCGGA TGCCACGGCC 
AACGAGTTCT GCTGCCGCGG CTACTGCATC GACCTGCTGA TCGAACTGTC CAAACGGATC 
AACTTCACCT ACGACCTGGC CCTCTCGCCG GATGGTCAGT TTGGGCACTA CATCCTTCGG 
AACAACACGG GAGCGATGAC GTTGCGCAAG GAGTGGACGG GCCTCATCGG GGAGCTGGTC 
AACGAACGTG CCGACATGAT CGTGGCACCA CTAACCATCA ATCCGGAGCG TGCCGAGTAC 
ATTGAATTCT CGAAGCCATT CAAATACCAA GGCATTACAA TACTCGAGAA GAAACCGTCA 
CGATCGAGTA CTCTCGTGTC TTTCTTGCAG CCGTTTAGTA ACACGCTATG GATCTTGGTA 
ATGGTGTCGG TCCATGTTGT GGCGCTCGTG CTCTACCTGC TGGACAGATT CTCGCCGTTC 
GGACGCTTCA AGCTCTCGCA CTCGGACAGC AACGAGGAGA AGGCCCTCAA TCTCAGCTCC 
GCGGTGTGGT TCGCCTGGGG AGTCCTGCTG AACAGCGGGA TCGGCGAGGG CACGCCCCGG 
AGCTTCTCCG CCCGGGTGCT GGGCATGGTC TGGGCCGGAT TTGCTATGAT CATCGTTGCC 
TCGTACACCG CCAACCTGGC CGCCTTCCTC GTGCTGGAGC GGCCCAAGAC CAAGTTGAGC 
GGCATCAACG ACGCCCGCTT GCGGAACACC ATGGAGAACC TGACCTGCGC CACCGTCAAG 
GGCTCCTCCG TGGACATGTA CTTCCGTCGC CAGGTGGAGC TCTCCAACAT GTATCGCACC 
ATGGAGGCGA ACAACTACGC CACCGCCGAG CAGGCCATCC AGGATGTGAA GAAGGGCAAG 
CTTATGGCCT TCATCTGGGA TTCATCTCGG CTGGAGTACG AGGCCTCCAA GGATTGCGAA 
CTAGTTACAG CCGGAGAGCT GTTCGGACGA AGTGGGTACG GGATTGGACT GCAAAAGGGC 
TCGCCCTGGA CGGATGCGGT CACGCTGGCC ATCTTGGAGT TCCACGAAAG CGGTTTCATG 
GAAAAATTGG ACAAACAGTG GATCTTTCAC GGACACGTCC AGCAGAACTG CGAGCTGTTC 
GAGAAGACGC CCAACACTCT TGGGCTTAAA AACATGGCGG GAGTGTTCAT ACTGGTGGGT 
GTGGGCATTG CCGGCGGCGT GGGCCTGATC ATCATCGAGG TCATCTACAA GAAGCACCAG 
GTGAAGAAGC AGAAGCGCCT GGACATTGCC CGACATGCGG CGGACAAGTG GCGGGGCACC 
ATAGAGAAAC GAAAGACTAT TCGTGCCTCG CTGGCGATGC AGCGACAGTA CAACGTGGGG 
CTGAACTCCA CCCATGCCCC GGGCACCATC AGTCTGGCAG TGGACAAGCG CCGGTATCCT 
CGCCTCGGTC AGCGACTGGG ACCGGAGAGA GCCTGGCCAG GAGACGCCGC CGACGTTCTG 
CGCATCCGTC GGCCCTACGA ACTGGGCAAT CCTGGCCAGA GTCCGAAGGT GATGGCTGCC 
AACCAGCCGG GAATGCCCAT GCCCATGCTG GGCAAGACAC GGCCCCAACA GAGTGTCCTG 
CCACCTCGCT ACTCGCCCGG ATACACCAGC GACGTGTCGC ATCTGGTCGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nmdar1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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