Usp32 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp32
Entrez Gene ID	40169
Full Name	Ubiquitin specific protease 32
Synonyms	CG8334,Dmel\CG8334,Q9VW49,USP15,usp32
General protein information	Preferred Names Ubiquitin specific protease 32 Names CG8334 gene product from transcript CG8334-RB CG8334-PB CG8334-PC CG8334-PD CG8334-PE Usp32-PB Usp32-PC Usp32-PD Usp32-PE ubiquitin specific protease 32
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76D3-76D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275148.2	NP_001262077.1	ubiquitin specific protease 32, isoform E
NM_001275146.2	NP_001262075.1	ubiquitin specific protease 32, isoform C
NM_001275147.2	NP_001262076.1	ubiquitin specific protease 32, isoform D
NM_001275145.2	NP_001262074.1	ubiquitin specific protease 32, isoform B

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC716857	XP_002808172.1
Canis lupus familiaris (dog)	USP32	XP_005624819.1
Bos taurus (cattle)	USP32	XP_002695689.2
Gallus gallus (chicken)	USP32	XP_004946797.1
Drosophila melanogaster (fruit fly)	CG8334	NP_001262075.1
Caenorhabditis elegans (roundworm)	cyk-3	NP_001022992.1
Xenopus tropicalis (tropical clawed frog)	usp32	XP_002933869.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001209	XP_321947.5
Mus musculus (house mouse)	Usp32	NP_001025105.1
Rattus norvegicus (Norway rat)	Usp32	NP_001100502.1
Danio rerio (zebrafish)	usp32	XP_686236.6
Homo sapiens (human)	USP32	NP_115971.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Usp32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa21104	NM_001275145.2 Latest version!	Drosophila melanogaster ubiquitin specific protease 32, transcript variant B (Usp32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa21105	NM_001275148.2 Latest version!	Drosophila melanogaster ubiquitin specific protease 32, transcript variant E (Usp32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa21106	NM_001275146.2 Latest version!	Drosophila melanogaster ubiquitin specific protease 32, transcript variant C (Usp32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFa21107	NM_001275147.2 Latest version!	Drosophila melanogaster ubiquitin specific protease 32, transcript variant D (Usp32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp32 ( NM_001275145.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262074.1 Drosophila melanogaster ubiquitin specific protease 32, transcript variant B (Usp32), mRNA.

Price & Availability↑

CloneID	OFa21104
Clone ID Related Accession (Same CDS sequence)	NM_001275145.2
Accession Version	NM_001275145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 32, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001275145.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGTAA GCCGGTGCTT TGGAAAGAGA 
GAGTGCATGC CCCACAGCCT ATTTTGCTTC AAGGAGTCGC TAATTCTGAG CTCCAGCCCG 
GAGAACACAT TTATGCACGG CGCTGCCGCC CAACAGAAGC GCGTATCGTC CGCCAAGCTG 
CTGCACACGG AGAGTAATAC CAGCTCCATG TCCTACACGA ATCATTCCGG CGAAAATTCC 
ATGGAGAGCT CGCTGACCGA ACCCATTCCA TTGGCGGACC TGGAGCCGGT TAGCAGCCGT 
AATGGCAGTG GCGGCGAGGA CTGCTCCTAC CGGACGTCGC CAAACGATTC CAGTGGCCTC 
AGCACGGGCC ACACCCTGGG CGCCAGTTTG GACGTGGACG AGCAGGCGGA AGAGGGCAAC 
GCGGAGGATC ACGATCAGCC GGATCAGATC ACAACCTCGC AGCCGGAGAC CAGTAGCGGG 
GTCTACTCTC GTCGCTCCTC CCAGCCGCCG CACAAGGCGG GCAAGTATTT GGTAGCCGTA 
CATCGAAAGA TAACGCGGCA TGACAGCTAC TTCCTGTCCT ACCACAAGAC GAGACCAAGT 
CTCTTTGGCG TTCCGCTGCT CATTCCAAAC AGCGAGGGTG GCACCCACAA GGATCTTTAC 
TGCGCCGTGT GGCTGCAGGT GAGCAGGCTA CTCAGCCCAT TGCCTGCCAC CACGGAACAG 
GCGAATCATG CCGCCGATTG TGATGACAGC CTGGGCTACG ATTTCCCCTT CACGTTGCGT 
GCCGTCAAGG CAGATGGTCT AACCTGCGCT ATTTGCCCTT GGTCAAGCTT CTGTCGCGGA 
TGCGAGATTC GATGCAACAA TGATTATGTA CTCCAGGGAG CCCTGCCGCC CATCAATGCC 
GCCGCCAGTA ATACATCAAC GCCAAAAATG AATGCTAAAT TCCCATCGCT ACCAAATCTG 
GAGGCTAAAC GGACGCCGGA ATATACCGCC TCCTTGTCGT ACACACCGAC AACCAAATAC 
TTTGAAGACT TCACCATTGC CATCGACTGG GATCCGACCG CCCTGCACTT GCGCTACCAA 
AGCACCTTGG AGCGTCTGTG GGTGGATCAC GAGACCATTG CCATAAGTCG GCGGGAGCAA 
GTGGAGCCCG TAGACCTGAA TCATTGCCTG CGCGCCTTCA CCTCGGAGGA GAAACTGGAG 
CAGTGGTATC ACTGCAGTCA TTGCAAGGGC AAAAAACCCG CCACCAAGAA GCTCCAGATC 
TGGAAGCTAC CTCCGATATT GATTGTTCAC TTGAAGCGGT TTAACTGCGT CAACGGCAAA 
TGGGTGAAGT CACAGAAAGT GGTCCACTTT CCTTTCGATG ACTTCGATCC TACTCCGTAT 
CTGGCCTCAG TCCCTCAGGA GACGATACTG CGGCACAAGG AGCTGCTGGA GCTAAAGAAT 
GATGCAGAAA TGACGATGGC AACTAACGAA GTTGTTAGCG AATTGGACGA GATTGATGCA 
CCCAGTAAGG AGGTTAAGGA AGAACTACCC AATCAAACGG GGTCCACAAA GGCTACGGCG 
TCGCCACCGC CCACAGGGAA TATTCTGCGC CAGAGCAAGA CCAAAAATGC TGTCCGGCGA 
CAACGCCTCA TCTCTACGAG TCTCACAAAA ACGCCTATCG TTGATGGCGA GTTCGAAGAC 
TATCACCAAC ACCGACTTAA ACCAGATGTG GATCAGTTCG ATCCCAGATA CCGACTCTAT 
GCTGTTGTGT CTCACTCGGG CATGCTGAAC GGAGGTCACT ACATTTCCTA TGCTTCGAAT 
GCAACTGGTT CCTGGTACTG CTATAACGAC AGCTCCTGCC GCGAAATATC CCAGAAGCCG 
GTCATCGATC CAAGTGCTGC ATATCTGCTG TTCTACGAAC GCAAGGGCCT GGACTACGAG 
CCTTACCTAC CGAACATCGA GGGACGGACG CTGCCCAATA CCGCCAGTGT GCCACTCGAG 
GTGGACGAGA CCGAGGGAGA GCTGAAGAAG CTGTGCTCAA TTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262074.1
CDS	448..5595
Translation	MLYAACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNLAPFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNCVSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCFGKRECMPHSLFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEVDETEGELKKLCSIS

Target ORF information:

RefSeq Version	NM_001275145.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 32, transcript variant B (Usp32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275145.2

ORF Insert Sequence:

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGTAA GCCGGTGCTT TGGAAAGAGA 
GAGTGCATGC CCCACAGCCT ATTTTGCTTC AAGGAGTCGC TAATTCTGAG CTCCAGCCCG 
GAGAACACAT TTATGCACGG CGCTGCCGCC CAACAGAAGC GCGTATCGTC CGCCAAGCTG 
CTGCACACGG AGAGTAATAC CAGCTCCATG TCCTACACGA ATCATTCCGG CGAAAATTCC 
ATGGAGAGCT CGCTGACCGA ACCCATTCCA TTGGCGGACC TGGAGCCGGT TAGCAGCCGT 
AATGGCAGTG GCGGCGAGGA CTGCTCCTAC CGGACGTCGC CAAACGATTC CAGTGGCCTC 
AGCACGGGCC ACACCCTGGG CGCCAGTTTG GACGTGGACG AGCAGGCGGA AGAGGGCAAC 
GCGGAGGATC ACGATCAGCC GGATCAGATC ACAACCTCGC AGCCGGAGAC CAGTAGCGGG 
GTCTACTCTC GTCGCTCCTC CCAGCCGCCG CACAAGGCGG GCAAGTATTT GGTAGCCGTA 
CATCGAAAGA TAACGCGGCA TGACAGCTAC TTCCTGTCCT ACCACAAGAC GAGACCAAGT 
CTCTTTGGCG TTCCGCTGCT CATTCCAAAC AGCGAGGGTG GCACCCACAA GGATCTTTAC 
TGCGCCGTGT GGCTGCAGGT GAGCAGGCTA CTCAGCCCAT TGCCTGCCAC CACGGAACAG 
GCGAATCATG CCGCCGATTG TGATGACAGC CTGGGCTACG ATTTCCCCTT CACGTTGCGT 
GCCGTCAAGG CAGATGGTCT AACCTGCGCT ATTTGCCCTT GGTCAAGCTT CTGTCGCGGA 
TGCGAGATTC GATGCAACAA TGATTATGTA CTCCAGGGAG CCCTGCCGCC CATCAATGCC 
GCCGCCAGTA ATACATCAAC GCCAAAAATG AATGCTAAAT TCCCATCGCT ACCAAATCTG 
GAGGCTAAAC GGACGCCGGA ATATACCGCC TCCTTGTCGT ACACACCGAC AACCAAATAC 
TTTGAAGACT TCACCATTGC CATCGACTGG GATCCGACCG CCCTGCACTT GCGCTACCAA 
AGCACCTTGG AGCGTCTGTG GGTGGATCAC GAGACCATTG CCATAAGTCG GCGGGAGCAA 
GTGGAGCCCG TAGACCTGAA TCATTGCCTG CGCGCCTTCA CCTCGGAGGA GAAACTGGAG 
CAGTGGTATC ACTGCAGTCA TTGCAAGGGC AAAAAACCCG CCACCAAGAA GCTCCAGATC 
TGGAAGCTAC CTCCGATATT GATTGTTCAC TTGAAGCGGT TTAACTGCGT CAACGGCAAA 
TGGGTGAAGT CACAGAAAGT GGTCCACTTT CCTTTCGATG ACTTCGATCC TACTCCGTAT 
CTGGCCTCAG TCCCTCAGGA GACGATACTG CGGCACAAGG AGCTGCTGGA GCTAAAGAAT 
GATGCAGAAA TGACGATGGC AACTAACGAA GTTGTTAGCG AATTGGACGA GATTGATGCA 
CCCAGTAAGG AGGTTAAGGA AGAACTACCC AATCAAACGG GGTCCACAAA GGCTACGGCG 
TCGCCACCGC CCACAGGGAA TATTCTGCGC CAGAGCAAGA CCAAAAATGC TGTCCGGCGA 
CAACGCCTCA TCTCTACGAG TCTCACAAAA ACGCCTATCG TTGATGGCGA GTTCGAAGAC 
TATCACCAAC ACCGACTTAA ACCAGATGTG GATCAGTTCG ATCCCAGATA CCGACTCTAT 
GCTGTTGTGT CTCACTCGGG CATGCTGAAC GGAGGTCACT ACATTTCCTA TGCTTCGAAT 
GCAACTGGTT CCTGGTACTG CTATAACGAC AGCTCCTGCC GCGAAATATC CCAGAAGCCG 
GTCATCGATC CAAGTGCTGC ATATCTGCTG TTCTACGAAC GCAAGGGCCT GGACTACGAG 
CCTTACCTAC CGAACATCGA GGGACGGACG CTGCCCAATA CCGCCAGTGT GCCACTCGAG 
GTGGACGAGA CCGAGGGAGA GCTGAAGAAG CTGTGCTCAA TTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp32 ( NM_001275148.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262077.1 Drosophila melanogaster ubiquitin specific protease 32, transcript variant E (Usp32), mRNA.

Price & Availability↑

CloneID	OFa21105
Clone ID Related Accession (Same CDS sequence)	NM_001275148.2
Accession Version	NM_001275148.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 32, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001275148.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGTAA GCCGGTGCTT TGGAAAGAGA 
GAGTGCATGC CCCACAGCCT ATTTTGCTTC AAGGAGTCGC TAATTCTGAG CTCCAGCCCG 
GAGAACACAT TTATGCACGG CGCTGCCGCC CAACAGAAGC GCGTATCGTC CGCCAAGCTG 
CTGCACACGG AGAGTAATAC CAGCTCCATG TCCTACACGA ATCATTCCGG CGAAAATTCC 
ATGGAGAGCT CGCTGACCGA ACCCATTCCA TTGGCGGACC TGGAGCCGGT TAGCAGCCGT 
AATGGCAGTG GCGGCGAGGA CTGCTCCTAC CGGACGTCGC CAAACGATTC CAGTGGCCTC 
AGCACGGGCC ACACCCTGGG CGCCAGTTTG GACGTGGACG AGCAGGCGGA AGAGGGCAAC 
GCGGAGGATC ACGATCAGCC GGATCAGATC ACAACCTCGC AGCCGGAGAC CAGTAGCGGG 
GTCTACTCTC GTCGCTCCTC CCAGCCGCCG CACAAGGCGG GCAAGTATTT GGTAGCCGTA 
CATCGAAAGA TAACGCGGCA TGACAGCTAC TTCCTGTCCT ACCACAAGAC GAGACCAAGT 
CTCTTTGGCG TTCCGCTGCT CATTCCAAAC AGCGAGGGTG GCACCCACAA GGATCTTTAC 
TGCGCCGTGT GGCTGCAGGT GAGCAGGCTA CTCAGCCCAT TGCCTGCCAC CACGGAACAG 
GCGAATCATG CCGCCGATTG CCTGGGCTAC GATTTCCCCT TCACGTTGCG TGCCGTCAAG 
GCAGATGGTC TAACCTGCGC TATTTGCCCT TGGTCAAGCT TCTGTCGCGG ATGCGAGATT 
CGATGCAACA ATGATTATGT ACTCCAGGGA GCCCTGCCGC CCATCAATGC CGCCGCCAGT 
AATACATCAA CGCCAAAAAT GAATGCTAAA TTCCCATCGC TACCAAATCT GGAGGCTAAA 
CGGACGCCGG AATATACCGC CTCCTTGTCG TACACACCGA CAACCAAATA CTTTGAAGAC 
TTCACCATTG CCATCGACTG GGATCCGACC GCCCTGCACT TGCGCTACCA AAGCACCTTG 
GAGCGTCTGT GGGTGGATCA CGAGACCATT GCCATAAGTC GGCGGGAGCA AGTGGAGCCC 
GTAGACCTGA ATCATTGCCT GCGCGCCTTC ACCTCGGAGG AGAAACTGGA GCAGTGGTAT 
CACTGCAGTC ATTGCAAGGG CAAAAAACCC GCCACCAAGA AGCTCCAGAT CTGGAAGCTA 
CCTCCGATAT TGATTGTTCA CTTGAAGCGG TTTAACTGCG TCAACGGCAA ATGGGTGAAG 
TCACAGAAAG TGGTCCACTT TCCTTTCGAT GACTTCGATC CTACTCCGTA TCTGGCCTCA 
GTCCCTCAGG AGACGATACT GCGGCACAAG GAGCTGCTGG AGCTAAAGAA TGATGCAGAA 
ATGACGATGG CAACTAACGA AGTTGTTAGC GAATTGGACG AGATTGATGC ACCCAGTAAG 
GAGGTTAAGG AAGAACTACC CAATCAAACG GGGTCCACAA AGGCTACGGC GTCGCCACCG 
CCCACAGGGA ATATTCTGCG CCAGAGCAAG ACCAAAAATG CTGTCCGGCG ACAACGCCTC 
ATCTCTACGA GTCTCACAAA AACGCCTATC GTTGATGGCG AGTTCGAAGA CTATCACCAA 
CACCGACTTA AACCAGATGT GGATCAGTTC GATCCCAGAT ACCGACTCTA TGCTGTTGTG 
TCTCACTCGG GCATGCTGAA CGGAGGTCAC TACATTTCCT ATGCTTCGAA TGCAACTGGT 
TCCTGGTACT GCTATAACGA CAGCTCCTGC CGCGAAATAT CCCAGAAGCC GGTCATCGAT 
CCAAGTGCTG CATATCTGCT GTTCTACGAA CGCAAGGGCC TGGACTACGA GCCTTACCTA 
CCGAACATCG AGGGACGGAC GCTGCCCAAT ACCGCCAGTG TGCCACTCGA GGTGGACGAG 
ACCGAGGGAG AGCTGAAGAA GCTGTGCTCA ATTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262077.1
CDS	448..5586
Translation	MLYAACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNLAPFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNCVSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCFGKRECMPHSLFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEVDETEGELKKLCSIS

Target ORF information:

RefSeq Version	NM_001275148.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 32, transcript variant E (Usp32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275148.2

ORF Insert Sequence:

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGTAA GCCGGTGCTT TGGAAAGAGA 
GAGTGCATGC CCCACAGCCT ATTTTGCTTC AAGGAGTCGC TAATTCTGAG CTCCAGCCCG 
GAGAACACAT TTATGCACGG CGCTGCCGCC CAACAGAAGC GCGTATCGTC CGCCAAGCTG 
CTGCACACGG AGAGTAATAC CAGCTCCATG TCCTACACGA ATCATTCCGG CGAAAATTCC 
ATGGAGAGCT CGCTGACCGA ACCCATTCCA TTGGCGGACC TGGAGCCGGT TAGCAGCCGT 
AATGGCAGTG GCGGCGAGGA CTGCTCCTAC CGGACGTCGC CAAACGATTC CAGTGGCCTC 
AGCACGGGCC ACACCCTGGG CGCCAGTTTG GACGTGGACG AGCAGGCGGA AGAGGGCAAC 
GCGGAGGATC ACGATCAGCC GGATCAGATC ACAACCTCGC AGCCGGAGAC CAGTAGCGGG 
GTCTACTCTC GTCGCTCCTC CCAGCCGCCG CACAAGGCGG GCAAGTATTT GGTAGCCGTA 
CATCGAAAGA TAACGCGGCA TGACAGCTAC TTCCTGTCCT ACCACAAGAC GAGACCAAGT 
CTCTTTGGCG TTCCGCTGCT CATTCCAAAC AGCGAGGGTG GCACCCACAA GGATCTTTAC 
TGCGCCGTGT GGCTGCAGGT GAGCAGGCTA CTCAGCCCAT TGCCTGCCAC CACGGAACAG 
GCGAATCATG CCGCCGATTG CCTGGGCTAC GATTTCCCCT TCACGTTGCG TGCCGTCAAG 
GCAGATGGTC TAACCTGCGC TATTTGCCCT TGGTCAAGCT TCTGTCGCGG ATGCGAGATT 
CGATGCAACA ATGATTATGT ACTCCAGGGA GCCCTGCCGC CCATCAATGC CGCCGCCAGT 
AATACATCAA CGCCAAAAAT GAATGCTAAA TTCCCATCGC TACCAAATCT GGAGGCTAAA 
CGGACGCCGG AATATACCGC CTCCTTGTCG TACACACCGA CAACCAAATA CTTTGAAGAC 
TTCACCATTG CCATCGACTG GGATCCGACC GCCCTGCACT TGCGCTACCA AAGCACCTTG 
GAGCGTCTGT GGGTGGATCA CGAGACCATT GCCATAAGTC GGCGGGAGCA AGTGGAGCCC 
GTAGACCTGA ATCATTGCCT GCGCGCCTTC ACCTCGGAGG AGAAACTGGA GCAGTGGTAT 
CACTGCAGTC ATTGCAAGGG CAAAAAACCC GCCACCAAGA AGCTCCAGAT CTGGAAGCTA 
CCTCCGATAT TGATTGTTCA CTTGAAGCGG TTTAACTGCG TCAACGGCAA ATGGGTGAAG 
TCACAGAAAG TGGTCCACTT TCCTTTCGAT GACTTCGATC CTACTCCGTA TCTGGCCTCA 
GTCCCTCAGG AGACGATACT GCGGCACAAG GAGCTGCTGG AGCTAAAGAA TGATGCAGAA 
ATGACGATGG CAACTAACGA AGTTGTTAGC GAATTGGACG AGATTGATGC ACCCAGTAAG 
GAGGTTAAGG AAGAACTACC CAATCAAACG GGGTCCACAA AGGCTACGGC GTCGCCACCG 
CCCACAGGGA ATATTCTGCG CCAGAGCAAG ACCAAAAATG CTGTCCGGCG ACAACGCCTC 
ATCTCTACGA GTCTCACAAA AACGCCTATC GTTGATGGCG AGTTCGAAGA CTATCACCAA 
CACCGACTTA AACCAGATGT GGATCAGTTC GATCCCAGAT ACCGACTCTA TGCTGTTGTG 
TCTCACTCGG GCATGCTGAA CGGAGGTCAC TACATTTCCT ATGCTTCGAA TGCAACTGGT 
TCCTGGTACT GCTATAACGA CAGCTCCTGC CGCGAAATAT CCCAGAAGCC GGTCATCGAT 
CCAAGTGCTG CATATCTGCT GTTCTACGAA CGCAAGGGCC TGGACTACGA GCCTTACCTA 
CCGAACATCG AGGGACGGAC GCTGCCCAAT ACCGCCAGTG TGCCACTCGA GGTGGACGAG 
ACCGAGGGAG AGCTGAAGAA GCTGTGCTCA ATTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp32 ( NM_001275146.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262075.1 Drosophila melanogaster ubiquitin specific protease 32, transcript variant C (Usp32), mRNA.

Price & Availability↑

CloneID	OFa21106
Clone ID Related Accession (Same CDS sequence)	NM_001275146.2
Accession Version	NM_001275146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 32, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001275146.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGAGT CGCTAATTCT GAGCTCCAGC 
CCGGAGAACA CATTTATGCA CGGCGCTGCC GCCCAACAGA AGCGCGTATC GTCCGCCAAG 
CTGCTGCACA CGGAGAGTAA TACCAGCTCC ATGTCCTACA CGAATCATTC CGGCGAAAAT 
TCCATGGAGA GCTCGCTGAC CGAACCCATT CCATTGGCGG ACCTGGAGCC GGTTAGCAGC 
CGTAATGGCA GTGGCGGCGA GGACTGCTCC TACCGGACGT CGCCAAACGA TTCCAGTGGC 
CTCAGCACGG GCCACACCCT GGGCGCCAGT TTGGACGTGG ACGAGCAGGC GGAAGAGGGC 
AACGCGGAGG ATCACGATCA GCCGGATCAG ATCACAACCT CGCAGCCGGA GACCAGTAGC 
GGGGTCTACT CTCGTCGCTC CTCCCAGCCG CCGCACAAGG CGGGCAAGTA TTTGGTAGCC 
GTACATCGAA AGATAACGCG GCATGACAGC TACTTCCTGT CCTACCACAA GACGAGACCA 
AGTCTCTTTG GCGTTCCGCT GCTCATTCCA AACAGCGAGG GTGGCACCCA CAAGGATCTT 
TACTGCGCCG TGTGGCTGCA GGTGAGCAGG CTACTCAGCC CATTGCCTGC CACCACGGAA 
CAGGCGAATC ATGCCGCCGA TTGTGATGAC AGCCTGGGCT ACGATTTCCC CTTCACGTTG 
CGTGCCGTCA AGGCAGATGG TCTAACCTGC GCTATTTGCC CTTGGTCAAG CTTCTGTCGC 
GGATGCGAGA TTCGATGCAA CAATGATTAT GTACTCCAGG GAGCCCTGCC GCCCATCAAT 
GCCGCCGCCA GTAATACATC AACGCCAAAA ATGAATGCTA AATTCCCATC GCTACCAAAT 
CTGGAGGCTA AACGGACGCC GGAATATACC GCCTCCTTGT CGTACACACC GACAACCAAA 
TACTTTGAAG ACTTCACCAT TGCCATCGAC TGGGATCCGA CCGCCCTGCA CTTGCGCTAC 
CAAAGCACCT TGGAGCGTCT GTGGGTGGAT CACGAGACCA TTGCCATAAG TCGGCGGGAG 
CAAGTGGAGC CCGTAGACCT GAATCATTGC CTGCGCGCCT TCACCTCGGA GGAGAAACTG 
GAGCAGTGGT ATCACTGCAG TCATTGCAAG GGCAAAAAAC CCGCCACCAA GAAGCTCCAG 
ATCTGGAAGC TACCTCCGAT ATTGATTGTT CACTTGAAGC GGTTTAACTG CGTCAACGGC 
AAATGGGTGA AGTCACAGAA AGTGGTCCAC TTTCCTTTCG ATGACTTCGA TCCTACTCCG 
TATCTGGCCT CAGTCCCTCA GGAGACGATA CTGCGGCACA AGGAGCTGCT GGAGCTAAAG 
AATGATGCAG AAATGACGAT GGCAACTAAC GAAGTTGTTA GCGAATTGGA CGAGATTGAT 
GCACCCAGTA AGGAGGTTAA GGAAGAACTA CCCAATCAAA CGGGGTCCAC AAAGGCTACG 
GCGTCGCCAC CGCCCACAGG GAATATTCTG CGCCAGAGCA AGACCAAAAA TGCTGTCCGG 
CGACAACGCC TCATCTCTAC GAGTCTCACA AAAACGCCTA TCGTTGATGG CGAGTTCGAA 
GACTATCACC AACACCGACT TAAACCAGAT GTGGATCAGT TCGATCCCAG ATACCGACTC 
TATGCTGTTG TGTCTCACTC GGGCATGCTG AACGGAGGTC ACTACATTTC CTATGCTTCG 
AATGCAACTG GTTCCTGGTA CTGCTATAAC GACAGCTCCT GCCGCGAAAT ATCCCAGAAG 
CCGGTCATCG ATCCAAGTGC TGCATATCTG CTGTTCTACG AACGCAAGGG CCTGGACTAC 
GAGCCTTACC TACCGAACAT CGAGGGACGG ACGCTGCCCA ATACCGCCAG TGTGCCACTC 
GAGGTGGACG AGACCGAGGG AGAGCTGAAG AAGCTGTGCT CAATTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262075.1
CDS	448..5538
Translation	MLYAACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNLAPFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNCVSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEVDETEGELKKLCSIS

Target ORF information:

RefSeq Version	NM_001275146.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 32, transcript variant C (Usp32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275146.2

ORF Insert Sequence:

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGAGT CGCTAATTCT GAGCTCCAGC 
CCGGAGAACA CATTTATGCA CGGCGCTGCC GCCCAACAGA AGCGCGTATC GTCCGCCAAG 
CTGCTGCACA CGGAGAGTAA TACCAGCTCC ATGTCCTACA CGAATCATTC CGGCGAAAAT 
TCCATGGAGA GCTCGCTGAC CGAACCCATT CCATTGGCGG ACCTGGAGCC GGTTAGCAGC 
CGTAATGGCA GTGGCGGCGA GGACTGCTCC TACCGGACGT CGCCAAACGA TTCCAGTGGC 
CTCAGCACGG GCCACACCCT GGGCGCCAGT TTGGACGTGG ACGAGCAGGC GGAAGAGGGC 
AACGCGGAGG ATCACGATCA GCCGGATCAG ATCACAACCT CGCAGCCGGA GACCAGTAGC 
GGGGTCTACT CTCGTCGCTC CTCCCAGCCG CCGCACAAGG CGGGCAAGTA TTTGGTAGCC 
GTACATCGAA AGATAACGCG GCATGACAGC TACTTCCTGT CCTACCACAA GACGAGACCA 
AGTCTCTTTG GCGTTCCGCT GCTCATTCCA AACAGCGAGG GTGGCACCCA CAAGGATCTT 
TACTGCGCCG TGTGGCTGCA GGTGAGCAGG CTACTCAGCC CATTGCCTGC CACCACGGAA 
CAGGCGAATC ATGCCGCCGA TTGTGATGAC AGCCTGGGCT ACGATTTCCC CTTCACGTTG 
CGTGCCGTCA AGGCAGATGG TCTAACCTGC GCTATTTGCC CTTGGTCAAG CTTCTGTCGC 
GGATGCGAGA TTCGATGCAA CAATGATTAT GTACTCCAGG GAGCCCTGCC GCCCATCAAT 
GCCGCCGCCA GTAATACATC AACGCCAAAA ATGAATGCTA AATTCCCATC GCTACCAAAT 
CTGGAGGCTA AACGGACGCC GGAATATACC GCCTCCTTGT CGTACACACC GACAACCAAA 
TACTTTGAAG ACTTCACCAT TGCCATCGAC TGGGATCCGA CCGCCCTGCA CTTGCGCTAC 
CAAAGCACCT TGGAGCGTCT GTGGGTGGAT CACGAGACCA TTGCCATAAG TCGGCGGGAG 
CAAGTGGAGC CCGTAGACCT GAATCATTGC CTGCGCGCCT TCACCTCGGA GGAGAAACTG 
GAGCAGTGGT ATCACTGCAG TCATTGCAAG GGCAAAAAAC CCGCCACCAA GAAGCTCCAG 
ATCTGGAAGC TACCTCCGAT ATTGATTGTT CACTTGAAGC GGTTTAACTG CGTCAACGGC 
AAATGGGTGA AGTCACAGAA AGTGGTCCAC TTTCCTTTCG ATGACTTCGA TCCTACTCCG 
TATCTGGCCT CAGTCCCTCA GGAGACGATA CTGCGGCACA AGGAGCTGCT GGAGCTAAAG 
AATGATGCAG AAATGACGAT GGCAACTAAC GAAGTTGTTA GCGAATTGGA CGAGATTGAT 
GCACCCAGTA AGGAGGTTAA GGAAGAACTA CCCAATCAAA CGGGGTCCAC AAAGGCTACG 
GCGTCGCCAC CGCCCACAGG GAATATTCTG CGCCAGAGCA AGACCAAAAA TGCTGTCCGG 
CGACAACGCC TCATCTCTAC GAGTCTCACA AAAACGCCTA TCGTTGATGG CGAGTTCGAA 
GACTATCACC AACACCGACT TAAACCAGAT GTGGATCAGT TCGATCCCAG ATACCGACTC 
TATGCTGTTG TGTCTCACTC GGGCATGCTG AACGGAGGTC ACTACATTTC CTATGCTTCG 
AATGCAACTG GTTCCTGGTA CTGCTATAAC GACAGCTCCT GCCGCGAAAT ATCCCAGAAG 
CCGGTCATCG ATCCAAGTGC TGCATATCTG CTGTTCTACG AACGCAAGGG CCTGGACTAC 
GAGCCTTACC TACCGAACAT CGAGGGACGG ACGCTGCCCA ATACCGCCAG TGTGCCACTC 
GAGGTGGACG AGACCGAGGG AGAGCTGAAG AAGCTGTGCT CAATTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp32 ( NM_001275147.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262076.1 Drosophila melanogaster ubiquitin specific protease 32, transcript variant D (Usp32), mRNA.

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CloneID	OFa21107
Clone ID Related Accession (Same CDS sequence)	NM_001275147.2
Accession Version	NM_001275147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin specific protease 32, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001275147.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGAGT CGCTAATTCT GAGCTCCAGC 
CCGGAGAACA CATTTATGCA CGGCGCTGCC GCCCAACAGA AGCGCGTATC GTCCGCCAAG 
CTGCTGCACA CGGAGAGTAA TACCAGCTCC ATGTCCTACA CGAATCATTC CGGCGAAAAT 
TCCATGGAGA GCTCGCTGAC CGAACCCATT CCATTGGCGG ACCTGGAGCC GGTTAGCAGC 
CGTAATGGCA GTGGCGGCGA GGACTGCTCC TACCGGACGT CGCCAAACGA TTCCAGTGGC 
CTCAGCACGG GCCACACCCT GGGCGCCAGT TTGGACGTGG ACGAGCAGGC GGAAGAGGGC 
AACGCGGAGG ATCACGATCA GCCGGATCAG ATCACAACCT CGCAGCCGGA GACCAGTAGC 
GGGGTCTACT CTCGTCGCTC CTCCCAGCCG CCGCACAAGG CGGGCAAGTA TTTGGTAGCC 
GTACATCGAA AGATAACGCG GCATGACAGC TACTTCCTGT CCTACCACAA GACGAGACCA 
AGTCTCTTTG GCGTTCCGCT GCTCATTCCA AACAGCGAGG GTGGCACCCA CAAGGATCTT 
TACTGCGCCG TGTGGCTGCA GGTGAGCAGG CTACTCAGCC CATTGCCTGC CACCACGGAA 
CAGGCGAATC ATGCCGCCGA TTGCCTGGGC TACGATTTCC CCTTCACGTT GCGTGCCGTC 
AAGGCAGATG GTCTAACCTG CGCTATTTGC CCTTGGTCAA GCTTCTGTCG CGGATGCGAG 
ATTCGATGCA ACAATGATTA TGTACTCCAG GGAGCCCTGC CGCCCATCAA TGCCGCCGCC 
AGTAATACAT CAACGCCAAA AATGAATGCT AAATTCCCAT CGCTACCAAA TCTGGAGGCT 
AAACGGACGC CGGAATATAC CGCCTCCTTG TCGTACACAC CGACAACCAA ATACTTTGAA 
GACTTCACCA TTGCCATCGA CTGGGATCCG ACCGCCCTGC ACTTGCGCTA CCAAAGCACC 
TTGGAGCGTC TGTGGGTGGA TCACGAGACC ATTGCCATAA GTCGGCGGGA GCAAGTGGAG 
CCCGTAGACC TGAATCATTG CCTGCGCGCC TTCACCTCGG AGGAGAAACT GGAGCAGTGG 
TATCACTGCA GTCATTGCAA GGGCAAAAAA CCCGCCACCA AGAAGCTCCA GATCTGGAAG 
CTACCTCCGA TATTGATTGT TCACTTGAAG CGGTTTAACT GCGTCAACGG CAAATGGGTG 
AAGTCACAGA AAGTGGTCCA CTTTCCTTTC GATGACTTCG ATCCTACTCC GTATCTGGCC 
TCAGTCCCTC AGGAGACGAT ACTGCGGCAC AAGGAGCTGC TGGAGCTAAA GAATGATGCA 
GAAATGACGA TGGCAACTAA CGAAGTTGTT AGCGAATTGG ACGAGATTGA TGCACCCAGT 
AAGGAGGTTA AGGAAGAACT ACCCAATCAA ACGGGGTCCA CAAAGGCTAC GGCGTCGCCA 
CCGCCCACAG GGAATATTCT GCGCCAGAGC AAGACCAAAA ATGCTGTCCG GCGACAACGC 
CTCATCTCTA CGAGTCTCAC AAAAACGCCT ATCGTTGATG GCGAGTTCGA AGACTATCAC 
CAACACCGAC TTAAACCAGA TGTGGATCAG TTCGATCCCA GATACCGACT CTATGCTGTT 
GTGTCTCACT CGGGCATGCT GAACGGAGGT CACTACATTT CCTATGCTTC GAATGCAACT 
GGTTCCTGGT ACTGCTATAA CGACAGCTCC TGCCGCGAAA TATCCCAGAA GCCGGTCATC 
GATCCAAGTG CTGCATATCT GCTGTTCTAC GAACGCAAGG GCCTGGACTA CGAGCCTTAC 
CTACCGAACA TCGAGGGACG GACGCTGCCC AATACCGCCA GTGTGCCACT CGAGGTGGAC 
GAGACCGAGG GAGAGCTGAA GAAGCTGTGC TCAATTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262076.1
CDS	448..5529
Translation	MLYAACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNLAPFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNCVSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEVDETEGELKKLCSIS

Target ORF information:

RefSeq Version	NM_001275147.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin specific protease 32, transcript variant D (Usp32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275147.2

ORF Insert Sequence:

ATGTTATATG CCGCCTGTGG TGGCACCCAG CGTGGCATCT CCTTTAACGA CCTGCTCTGC 
GGTCTGGTCT TGATAACTCG TGGTACCCAA GCGGAGAAAA CCAAATTCCT GTGGAACCTG 
TACTGCAACG ATGCCGGCAC CTTCATCATC AAGTCGGACT ATGTGCGCAA CGTCAATCTG 
GCTCCCTTCG AGAGCGTCTC CCTATTCGCT CAAAGCGAAC GCGTCAATTT CGAGCAGTTC 
CAGGACTGGA TCATAAAGCA TCGCAATGCC ACTGTCCTCT CCAAATGGCT TCTGTCCGAC 
AACTGTGTTA GCCTCACATC GGAGCTGGAG ACGCCAACGT TTTACCAGAG CCTCGCTGGA 
GTGACGCATC TTGAGGAGAA GGACATTGGC GATTTGGAAA AGGAGTTTTG GCGTTTGAAA 
AACACTTCCC AAAATGGCCA AATCGACTTG CAGTTCCTGG GACCCCTGAT CAGTCCACCC 
ATACCAAAGA ATGCTCTAGC AGGTCTATTC AACGCCTTTG ATGAGAACCG CGACGGACAC 
ATCGACTTTA AGGAGCTGTG TTGCGGAGTA AGTGCCGCCT GCCGAGGACC GGGTGTGGAA 
AGGACACGAT TTTGCTTCAA AATCTTCGAT GTGGATCGAG ATGGCGTTCT TAGCCACGAT 
GAAACACTGC AGATGATCAA TGTTCTGCTA TTAGTAGCCA AGGAAAATCA GGAGTCCCAG 
CAATACAAGG ATCTGACCAA ACAGCTGGTG ATAAGTGATC TTCTGGAGTT TGGACAGAGA 
AGAAGTCCGG ATGGAACGCC TAGTAAGCTA ACCCGAGACA ATGTCTCGCT GACCGCTGAG 
GATTTTATGC TGTGGACCGT ACAGTGTGAT CTAAGACTCA TGCAGCCCTT GCTGGACCTC 
ATCTTCGAGC TGTGCCACAT CGTCTTTGGC CTGTGGCCCC AGTGCAAGCA CATGGAGAAC 
GATATCGTCC GGGGATGGTT GCGACGGGAG GAGCGCAGGC CGTACCGCGT TGGACAGTTT 
TGGTACTTGA TCACGCATGA CTGGTGGCTC AGTTGGATGC AATACACTCA GCACACGACG 
CACACCTGCG ACTATTGCAA GCGAACCGCC AGCCAGCGGA CCGCTGTTGA TGAGGCGCTC 
GTCTGCGATG AGAGCTTTAA CACCCACAGC TTGGAACAGC ACGATAGCTA CTCCCTGGGT 
TCTGGCACGG GATCGGCTTC CGGTTCCGGG TCGGCGAGTA GTGGCATCTC AGCTGGACGA 
CATTGCGGAC CCGTGCGACC AGGGCCTATT GACAACAGCA ATCTGATCAC CGCCAATCCG 
TTCCGGAATG TTCGAACTCT CACCGGCGAG GGTGGTCACC TCAAGCGGGA CACACCCCTG 
GTGCAGAACC ACGACTTTGA ACTGGTGCCC AAGTCACTGT GGAAGGCATT GAATCGATGG 
TATGGCGATA ATCTTCCACT GCCACGACAG GTCATCCAAC CGCCCAACTC CGATGTGGAA 
CTGGAGCTAT ACCCGCTGAA CCTGCGAATC CTGCTCCACC AGGCGCAGCC ATCCCAGACA 
GGCGTAGGCG GAGGCACTCA ACTTGGTAGT TGGGGCTCCA CGGTGAGCGG TGGCTACGGA 
GTGCTTGCCT CCGGTGGCGG ATATGCCGCC ATCGCTGTAA GCAGTGTACT CCAACCGCCG 
AAGCGATACT TGGCCTACAC GGCTGCCTTC AGCCGACTGG CCACGGTGCG TCAGGTGGGC 
GAGTTTTTGT GCGAGCAACT GCGTCTTAAG TCAGAAGATA TCCGGCTATG GCACGTTCCA 
CAGCTGGACA ACGGTGCCAT ATTGCTAGAA GAGGATGCCA TGTGCCTGAA AGAGTTGCTC 
ATTCGGGACA ACGACCAGCT GTTGCTGGAA ATCCGCAACA AAGATTTGAC CTGGCCGGAG 
GAACTTGGCT CCTTGGCCAC CGCTCAATGT GGCCAGGGTG CGGGAACACC GGGGGACCGG 
CGTCGCTTAA CACGCAGTTC AATTATGTCG GTACATGCGC CCGGAGCTAC AGGCCTGCAC 
AACCTGGGCA ACACCTGCTT CATGAACGCC GCTCTCCAGG TGCTGTTTAA TACCCAACCA 
CTGGCACAAT ACTTTCAGCG TGAGATGCAT CGCTTCGAGG TGAACGCGGC CAACAAACTG 
GGCACAAAGG GTCAGCTAGC CATGCGCTAT GCGGAGCTGC TCAAGGAGGT TTGGACGGCC 
ACGACTAGAT CTGTGGCCCC GCTGAAGCTA CGCTTTTGTG TCAACAAGTA TGCGCCGCAG 
TTCGCCGGTG GAGGTCAGCA CGATTCCCAG GAGCTGCTCG AGTGGCTGTT GGACGCTCTG 
CATGAGGACC TTAACCGTGT GATGGAGAAA CCGTACAGCG AGCTGAAGGA CTCGAATGGG 
CGCCCAGACA AAATAGTGGC CGCCGAGGCC TGGTCCCAGC ACCACGCCCG AAACCAGTCA 
ATTATCATTG ATCTGTTCTA CGGCCAGTTG AAATCCAAGG TCAGCTGTCT GGGTTGTGGA 
CACGAATCGG TGCGATTCGA TCCGTTTAGT CTGCTCAGTT TGCCGCTTCC GGTAGAGAAT 
TATATCTACT TTGAGGTCTT GGTTATTCTG TTGGATGGAA GCGTTCCAAT CAAATATGGT 
TTTCGTCTGA ACTCCGATTG CAAGTACTCG CACCTGAAAC ACAAGTTGTC CACCATGTGC 
TCCCTGCCTC CAAATCTAAT GCTCGTCTGC GAGCTATGGA ACTCACAAAT ACGCCAGGTG 
CTAAACGACG ACGAGAAACT GCGGACGCAG AGTGCCAAGG AGCTGTACGT CTATCAGCTG 
CCGGAACAAA GTATGCGAAC ACGATCCAAC TCAGGGCTTA GCATGCACAT CGAGCAGGGT 
CTTAAGGACA TACAGCGCAG TTCCGCTCTC ATCACGAGCG CCCAGGACTC GCTGTCCTCG 
CTGAGCACTT TGCAGACGTC CAGCCACCGC GCCTCGTCAC GAGTCCTGTG CAACGGACAC 
GTTTCGGGCC TGGATGTCGA AGGCGAGGCG GAGGTGGGCA CGGATGTGTC GCAATGTAAC 
AGCAACAGCA ATTACAATCC TATTGTAAGC ACTTACAGCG GAAACGGAAG CGGGGACAAC 
CAGGTGCACG AACTGCTGCC AGATGAGGCC GGAAAGGAGT CGCTAATTCT GAGCTCCAGC 
CCGGAGAACA CATTTATGCA CGGCGCTGCC GCCCAACAGA AGCGCGTATC GTCCGCCAAG 
CTGCTGCACA CGGAGAGTAA TACCAGCTCC ATGTCCTACA CGAATCATTC CGGCGAAAAT 
TCCATGGAGA GCTCGCTGAC CGAACCCATT CCATTGGCGG ACCTGGAGCC GGTTAGCAGC 
CGTAATGGCA GTGGCGGCGA GGACTGCTCC TACCGGACGT CGCCAAACGA TTCCAGTGGC 
CTCAGCACGG GCCACACCCT GGGCGCCAGT TTGGACGTGG ACGAGCAGGC GGAAGAGGGC 
AACGCGGAGG ATCACGATCA GCCGGATCAG ATCACAACCT CGCAGCCGGA GACCAGTAGC 
GGGGTCTACT CTCGTCGCTC CTCCCAGCCG CCGCACAAGG CGGGCAAGTA TTTGGTAGCC 
GTACATCGAA AGATAACGCG GCATGACAGC TACTTCCTGT CCTACCACAA GACGAGACCA 
AGTCTCTTTG GCGTTCCGCT GCTCATTCCA AACAGCGAGG GTGGCACCCA CAAGGATCTT 
TACTGCGCCG TGTGGCTGCA GGTGAGCAGG CTACTCAGCC CATTGCCTGC CACCACGGAA 
CAGGCGAATC ATGCCGCCGA TTGCCTGGGC TACGATTTCC CCTTCACGTT GCGTGCCGTC 
AAGGCAGATG GTCTAACCTG CGCTATTTGC CCTTGGTCAA GCTTCTGTCG CGGATGCGAG 
ATTCGATGCA ACAATGATTA TGTACTCCAG GGAGCCCTGC CGCCCATCAA TGCCGCCGCC 
AGTAATACAT CAACGCCAAA AATGAATGCT AAATTCCCAT CGCTACCAAA TCTGGAGGCT 
AAACGGACGC CGGAATATAC CGCCTCCTTG TCGTACACAC CGACAACCAA ATACTTTGAA 
GACTTCACCA TTGCCATCGA CTGGGATCCG ACCGCCCTGC ACTTGCGCTA CCAAAGCACC 
TTGGAGCGTC TGTGGGTGGA TCACGAGACC ATTGCCATAA GTCGGCGGGA GCAAGTGGAG 
CCCGTAGACC TGAATCATTG CCTGCGCGCC TTCACCTCGG AGGAGAAACT GGAGCAGTGG 
TATCACTGCA GTCATTGCAA GGGCAAAAAA CCCGCCACCA AGAAGCTCCA GATCTGGAAG 
CTACCTCCGA TATTGATTGT TCACTTGAAG CGGTTTAACT GCGTCAACGG CAAATGGGTG 
AAGTCACAGA AAGTGGTCCA CTTTCCTTTC GATGACTTCG ATCCTACTCC GTATCTGGCC 
TCAGTCCCTC AGGAGACGAT ACTGCGGCAC AAGGAGCTGC TGGAGCTAAA GAATGATGCA 
GAAATGACGA TGGCAACTAA CGAAGTTGTT AGCGAATTGG ACGAGATTGA TGCACCCAGT 
AAGGAGGTTA AGGAAGAACT ACCCAATCAA ACGGGGTCCA CAAAGGCTAC GGCGTCGCCA 
CCGCCCACAG GGAATATTCT GCGCCAGAGC AAGACCAAAA ATGCTGTCCG GCGACAACGC 
CTCATCTCTA CGAGTCTCAC AAAAACGCCT ATCGTTGATG GCGAGTTCGA AGACTATCAC 
CAACACCGAC TTAAACCAGA TGTGGATCAG TTCGATCCCA GATACCGACT CTATGCTGTT 
GTGTCTCACT CGGGCATGCT GAACGGAGGT CACTACATTT CCTATGCTTC GAATGCAACT 
GGTTCCTGGT ACTGCTATAA CGACAGCTCC TGCCGCGAAA TATCCCAGAA GCCGGTCATC 
GATCCAAGTG CTGCATATCT GCTGTTCTAC GAACGCAAGG GCCTGGACTA CGAGCCTTAC 
CTACCGAACA TCGAGGGACG GACGCTGCCC AATACCGCCA GTGTGCCACT CGAGGTGGAC 
GAGACCGAGG GAGAGCTGAA GAAGCTGTGC TCAATTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Usp32 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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