U4-U6-60K cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	U4-U6-60K
Entrez Gene ID	39955
Full Name	U4-U6 small nuclear riboprotein factor 60K
Synonyms	CG6322,Dmel\CG6322,Prp4,U4/U6-60K
General protein information	Preferred Names U4-U6 small nuclear riboprotein factor 60K Names CG6322 gene product from transcript CG6322-RA CG6322-PA U4-U6-60K-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 74D2-74D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_140733.3	NP_648990.1	U4-U6 small nuclear riboprotein factor 60K

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E04819g	XP_454163.1
Neurospora crassa	NCU11251	XP_001728376.1
Homo sapiens (human)	PRPF4	NP_001231855.1
Danio rerio (zebrafish)	prpf4	NP_956049.1
Caenorhabditis elegans (roundworm)	prp-4	NP_492363.1
Danio rerio (zebrafish)	LOC100334455	XP_002662201.1
Canis lupus familiaris (dog)	PRPF4	XP_532038.2
Drosophila melanogaster (fruit fly)	U4-U6-60K	NP_648990.1
Arabidopsis thaliana (thale cress)	AT2G05720	NP_178635.1
Eremothecium gossypii	AGOS_AEL269C	NP_984591.1
Schizosaccharomyces pombe (fission yeast)	SPAC227.12	NP_592966.1
Rattus norvegicus (Norway rat)	Prpf4	NP_001100129.1
Arabidopsis thaliana (thale cress)	LIS	NP_181681.1
Macaca mulatta (Rhesus monkey)	PRPF4	XP_001102859.1
Xenopus tropicalis (tropical clawed frog)	prpf4	NP_989058.1
Magnaporthe oryzae (rice blast fungus)	MGG_01259	XP_003714171.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002144	XP_308051.4
Pan troglodytes (chimpanzee)	PRPF4	XP_520198.3
Bos taurus (cattle)	PRPF4	NP_001029502.1
Mus musculus (house mouse)	Prpf4	NP_081573.1
Gallus gallus (chicken)	PRPF4	XP_415544.3
Saccharomyces cerevisiae (baker's yeast)	PRP4	NP_015504.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following U4-U6-60K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the U4-U6-60K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21058	NM_140733.3 Latest version!	Drosophila melanogaster U4-U6 small nuclear riboprotein factor 60K (U4-U6-60K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

U4-U6-60K ( NM_140733.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648990.1 Drosophila melanogaster U4-U6 small nuclear riboprotein factor 60K (U4-U6-60K), mRNA.

Price & Availability↑

CloneID	OFa21058
Clone ID Related Accession (Same CDS sequence)	NM_140733.3
Accession Version	NM_140733.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	U4-U6 small nuclear riboprotein factor 60K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140733.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGATG ACGACGATAT ACAGTACATT AAGCGGCAGC GAACGCTGCA CTATGGATCG 
CTCGAGGAAT CGGAACGCAA GCGGCAAAAT GCCGCGGCGT CTGGGGCAGC AGCAACAACA 
ACATCGGGAA CGACGGCATC GTCTGGGGCA GGAACAACAA CAACTGGAAC AGGCGGGCAA 
CTGGAAGACA TCGATTCCGA TGAGGATTAC GAGGAGTCCA CGAAAAAGAC AAGCAACGCC 
AAGCAGGCCG GAGCACCACC ACCCACAGCC GCTACTCTGG CCAACAAAAT TGACGATGAC 
TACTTCGATC TAGAGATGGA AATGGAGCGC GACAAGGTGG CGCTCCTTGA GGAATTTGAG 
CGGAAAAAGC GAGCACGACA GATAAATGTG TCCACAGACG ACACGGAGAT CAAGAGCAAT 
CTGCGCCAGC TAAACGAACC CATTTGCTAT TTTGGAGAAG GACCGGCGGA GCGAAGAAGA 
CGTCTTAAGG AACTCTTGGC TGGATTGGGC GAGAACGCCA TCAACAAGAG GCAGTATGAG 
GATGAGGAGC GCAAGCAGCA ACAGCGGGAG CAGGATCAGG CCACCTGGTA CCACGAGGGA 
CCCGATAGTT TGCGTATCGC TCGACTCTGG CTGGCGGATT ATTCCCTGCC GCGCGCCAAG 
GATCGCTTGG TACGAGCACG TGAGGCCCTT GAAGTGCCCA GCGCAGCACG TGCCGGCCGA 
ATGGTGGAGA TGCAGAAGAA GCTCCAGTCG CTGGCTCCAC TGTGCTCCCA GGTGGGTGAC 
ACACGGCCCG TGAGTAGTGC TGCCTTCAGT GAAGACTCCT CACTCCTTCT GACCTCCTCA 
TGGTCGGGTC TCTGCAAGCT GTGGAGCGTG CCCGATTGCG AGCTGAAGCA GACGCTGCGT 
GGACATGCCA GCTATGTGGG CGGAGTGGCT CTGCGGCCGG GAGTTAAGGC AGACGAGGAG 
AACGTGGTGG CCATGGCTTC CGGTGGCCAC GATGGCGCCG TCAAGCTGTG GGGCTTCAAC 
AACGAAGAGT CGATTGCCGA CATCACCGGC CACATGCCGC ATCGCGTCTC TAAGGTAGCC 
TTCCATCCTT CCGGCCGCTT CTTGGCCACC GCCTGCTACG ACTCTAGCTG GCGGCTGTGG 
GATCTGGAGC AAAAGACAGA GGTGCTTCAC CAGGAAGGGC ACGCAAAGCC GGTTCACTGC 
CTTAGCTATC ATTCCGACGG CAGTGTCCTG GTCACCGGCG GTCTGGATGC CTTTGGACGA 
GTGTGGGATC TGCGCACTGG GCGGTGCATC ATGTTTCTGG AGGGACACTT GGGCGCCGTT 
TTCGGCGTGG ACTTTTCTCC AAACGGATTC CACATAGCAA CAGGGTCCCA GGACAACACA 
TGTAAAATCT GGGACCTGAG GCGACGCCAG CCGGTCTATA CCATTCCTGC GCACACCAAT 
CTGATTTCGG ACGTGAAGTA CCAGCAGGAG TGCGGCAGCT TCCTGGTCAC CTGCTCCTAC 
GACTCGACCA CCAAGATCTG GTCCAACAAG ACGTGGCAGC CGTTGAAGAC GCTGCAGGGC 
CACGACAACA AGGTCATATC GGTGGACATC GCGCCCAACT CGCAGTATAT AGCCACGACC 
TCATTTGACC GCACATTTAA GCTGTGGTCG CCGGATAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648990.1
CDS	132..1793
Translation	MSDDDDIQYIKRQRTLHYGSLEESERKRQNAAASGAAATTTSGTTASSGAGTTTTGTGGQLEDIDSDEDYEESTKKTSNAKQAGAPPPTAATLANKIDDDYFDLEMEMERDKVALLEEFERKKRARQINVSTDDTEIKSNLRQLNEPICYFGEGPAERRRRLKELLAGLGENAINKRQYEDEERKQQQREQDQATWYHEGPDSLRIARLWLADYSLPRAKDRLVRAREALEVPSAARAGRMVEMQKKLQSLAPLCSQVGDTRPVSSAAFSEDSSLLLTSSWSGLCKLWSVPDCELKQTLRGHASYVGGVALRPGVKADEENVVAMASGGHDGAVKLWGFNNEESIADITGHMPHRVSKVAFHPSGRFLATACYDSSWRLWDLEQKTEVLHQEGHAKPVHCLSYHSDGSVLVTGGLDAFGRVWDLRTGRCIMFLEGHLGAVFGVDFSPNGFHIATGSQDNTCKIWDLRRRQPVYTIPAHTNLISDVKYQQECGSFLVTCSYDSTTKIWSNKTWQPLKTLQGHDNKVISVDIAPNSQYIATTSFDRTFKLWSPDS

Target ORF information:

RefSeq Version	NM_140733.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster U4-U6 small nuclear riboprotein factor 60K (U4-U6-60K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140733.3

ORF Insert Sequence:

ATGTCCGATG ACGACGATAT ACAGTACATT AAGCGGCAGC GAACGCTGCA CTATGGATCG 
CTCGAGGAAT CGGAACGCAA GCGGCAAAAT GCCGCGGCGT CTGGGGCAGC AGCAACAACA 
ACATCGGGAA CGACGGCATC GTCTGGGGCA GGAACAACAA CAACTGGAAC AGGCGGGCAA 
CTGGAAGACA TCGATTCCGA TGAGGATTAC GAGGAGTCCA CGAAAAAGAC AAGCAACGCC 
AAGCAGGCCG GAGCACCACC ACCCACAGCC GCTACTCTGG CCAACAAAAT TGACGATGAC 
TACTTCGATC TAGAGATGGA AATGGAGCGC GACAAGGTGG CGCTCCTTGA GGAATTTGAG 
CGGAAAAAGC GAGCACGACA GATAAATGTG TCCACAGACG ACACGGAGAT CAAGAGCAAT 
CTGCGCCAGC TAAACGAACC CATTTGCTAT TTTGGAGAAG GACCGGCGGA GCGAAGAAGA 
CGTCTTAAGG AACTCTTGGC TGGATTGGGC GAGAACGCCA TCAACAAGAG GCAGTATGAG 
GATGAGGAGC GCAAGCAGCA ACAGCGGGAG CAGGATCAGG CCACCTGGTA CCACGAGGGA 
CCCGATAGTT TGCGTATCGC TCGACTCTGG CTGGCGGATT ATTCCCTGCC GCGCGCCAAG 
GATCGCTTGG TACGAGCACG TGAGGCCCTT GAAGTGCCCA GCGCAGCACG TGCCGGCCGA 
ATGGTGGAGA TGCAGAAGAA GCTCCAGTCG CTGGCTCCAC TGTGCTCCCA GGTGGGTGAC 
ACACGGCCCG TGAGTAGTGC TGCCTTCAGT GAAGACTCCT CACTCCTTCT GACCTCCTCA 
TGGTCGGGTC TCTGCAAGCT GTGGAGCGTG CCCGATTGCG AGCTGAAGCA GACGCTGCGT 
GGACATGCCA GCTATGTGGG CGGAGTGGCT CTGCGGCCGG GAGTTAAGGC AGACGAGGAG 
AACGTGGTGG CCATGGCTTC CGGTGGCCAC GATGGCGCCG TCAAGCTGTG GGGCTTCAAC 
AACGAAGAGT CGATTGCCGA CATCACCGGC CACATGCCGC ATCGCGTCTC TAAGGTAGCC 
TTCCATCCTT CCGGCCGCTT CTTGGCCACC GCCTGCTACG ACTCTAGCTG GCGGCTGTGG 
GATCTGGAGC AAAAGACAGA GGTGCTTCAC CAGGAAGGGC ACGCAAAGCC GGTTCACTGC 
CTTAGCTATC ATTCCGACGG CAGTGTCCTG GTCACCGGCG GTCTGGATGC CTTTGGACGA 
GTGTGGGATC TGCGCACTGG GCGGTGCATC ATGTTTCTGG AGGGACACTT GGGCGCCGTT 
TTCGGCGTGG ACTTTTCTCC AAACGGATTC CACATAGCAA CAGGGTCCCA GGACAACACA 
TGTAAAATCT GGGACCTGAG GCGACGCCAG CCGGTCTATA CCATTCCTGC GCACACCAAT 
CTGATTTCGG ACGTGAAGTA CCAGCAGGAG TGCGGCAGCT TCCTGGTCAC CTGCTCCTAC 
GACTCGACCA CCAAGATCTG GTCCAACAAG ACGTGGCAGC CGTTGAAGAC GCTGCAGGGC 
CACGACAACA AGGTCATATC GGTGGACATC GCGCCCAACT CGCAGTATAT AGCCACGACC 
TCATTTGACC GCACATTTAA GCTGTGGTCG CCGGATAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

U4-U6-60K cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM