GAPsec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAPsec
Entrez Gene ID	38987
Full Name	GTPase activating protein, SECIS-dependent read-through
Synonyms	BEST:LD24460,CG5978,Dmel\CG5978,dGAPsec
General protein information	Preferred Names GTPase activating protein, SECIS-dependent read-through Names CG5978 gene product from transcript CG5978-RA CG5978-PA GAPsec-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66D8-66D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_139988.5	NP_648245.2	GTPase activating protein, SECIS-dependent read-through

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAC1952.17c	NP_594819.2
Pan troglodytes (chimpanzee)	TBC1D13	XP_520299.2
Drosophila melanogaster (fruit fly)	GAPsec	NP_648245.2
Arabidopsis thaliana (thale cress)	AT4G13730	NP_193109.1
Bos taurus (cattle)	TBC1D13	NP_001193644.1
Gallus gallus (chicken)	TBC1D13	XP_001233464.1
Danio rerio (zebrafish)	tbc1d13	NP_001107894.1
Arabidopsis thaliana (thale cress)	AT1G04830	NP_171975.2
Homo sapiens (human)	TBC1D13	NP_060671.3
Macaca mulatta (Rhesus monkey)	TBC1D13	XP_001118728.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005738	XP_315752.4
Canis lupus familiaris (dog)	TBC1D13	XP_537821.3
Mus musculus (house mouse)	Tbc1d13	NP_666364.1
Rattus norvegicus (Norway rat)	Tbc1d13	XP_575106.2
Oryza sativa (rice)	Os01g0908100	NP_001045140.1
Caenorhabditis elegans (roundworm)	tbc-13	NP_510095.2
Gallus gallus (chicken)	LOC100859585	XP_003642308.1
Xenopus tropicalis (tropical clawed frog)	tbc1d13	NP_001120521.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GAPsec gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAPsec cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17915	NM_139988.5 Latest version!	Drosophila melanogaster GTPase activating protein, SECIS-dependent read-through (GAPsec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAPsec ( NM_139988.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648245.2 Drosophila melanogaster GTPase activating protein, SECIS-dependent read-through (GAPsec), mRNA.

Price & Availability↑

CloneID	OFa17915
Clone ID Related Accession (Same CDS sequence)	NM_139988.5
Accession Version	NM_139988.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	GTPase activating protein, SECIS-dependent read-through
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139988.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTATTT TCAAGGCGCG AGTCAAGGAA TTTGAGGATG TTTTGGCGGG GCCACAGGAT 
TTAATTGACC TGAAACAACT CAGGAAACTG GCATTTAATG GTGTGCCCGA TGTGCAGAGT 
TTTCGAGCAC TCAGTTGGAA GCTACTCCTT GGCTATTTGG GGCCTAGGCG CAGTAGTTGG 
ACCACAACCT TGGCCCAAAA GCGAGCCCTG TATAAACAGT TCATCGAGGA GCTAGTCCTG 
CCCCCCGGAC ACTCGAGCAA TAGAGCTAGC GTAGACGGAG GAGATGGGGA CAAAGTCGAT 
TCGGGGGGCG TTGGCCTGCA AGATCATCCG CTGAGCGAGG GACCCGAAAG CGCGTGGAAC 
ACGTTCTTCA ATGATAACGA GTTTCTACTG CAAATCGACA AGGATGTCCG CCGCTTGTGC 
CCGGACATAT CCTTTTTCCA GCAGCCCACC GATTATCCCT GCGAGATTGT GGTCCACAGC 
AAAGGAGAGC ATGGACGAAG GCTCCACGAA CGGGTGGTTC CCGCCGTTCT AAGTTCGGCG 
AATGTGGAAC GCAAGGGCCT CGGCATGACA AAGATAAATT TGATTACAAA ACGCTCCGTT 
GAAAACTATG CCGCCATGGA GGAGGGCCAA GAGGCTCACT GGGAAGTTGT GCAACGCATT 
TTGTTCATCT ATGCCAAACT AAATCCCGGC CAGGGATATG TCCAGGGCAT GAACGAAATC 
GTGGGACCCA TTTACTATGT TATGGCCTCC GATCCTGACC TCACATATCG CGCCCACGCT 
GAGGCTGATT GTTTCTTTTG TTTTACCGCC CTGATGAGCG AGATAAGAGA CTTTTTTATT 
AAAACACTAG ACGACGCAGA AGGCGGCATC AAGTTCATGA TGGCCAGGCT ATCGAATATG 
CTGAAATCAA AGGACCTAAG CATTTACGAG CTACTCAGGA GCCAAGAGCT GCATCCGCAG 
TATTACAGCT TCAGGTGGCT CACACTGCTC CTGTCGCAGG AGTTTCCACT GCCAGATGTA 
CTGCGCATTT GGGATTCCGT GTTTGCGGAC GAACAGCGAT TTGATTTTCT GATAAAAATA 
TGCTGTTCAA TGATATTAAT CCAAAGGGAA GCAATTTTGG AAAACGACTT TGCCTCGAAT 
GTGAAACTCT TGCAAAACTA TCCGCCAATT GACATTAATG TTGTGATTGC TCATGCCGGA 
TCATTGGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648245.2
CDS	128..1339
Translation	MSIFKARVKEFEDVLAGPQDLIDLKQLRKLAFNGVPDVQSFRALSWKLLLGYLGPRRSSWTTTLAQKRALYKQFIEELVLPPGHSSNRASVDGGDGDKVDSGGVGLQDHPLSEGPESAWNTFFNDNEFLLQIDKDVRRLCPDISFFQQPTDYPCEIVVHSKGEHGRRLHERVVPAVLSSANVERKGLGMTKINLITKRSVENYAAMEEGQEAHWEVVQRILFIYAKLNPGQGYVQGMNEIVGPIYYVMASDPDLTYRAHAEADCFFCFTALMSEIRDFFIKTLDDAEGGIKFMMARLSNMLKSKDLSIYELLRSQELHPQYYSFRWLTLLLSQEFPLPDVLRIWDSVFADEQRFDFLIKICCSMILIQREAILENDFASNVKLLQNYPPIDINVVIAHAGSLA

Target ORF information:

RefSeq Version	NM_139988.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GTPase activating protein, SECIS-dependent read-through (GAPsec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139988.5

ORF Insert Sequence:

ATGTCTATTT TCAAGGCGCG AGTCAAGGAA TTTGAGGATG TTTTGGCGGG GCCACAGGAT 
TTAATTGACC TGAAACAACT CAGGAAACTG GCATTTAATG GTGTGCCCGA TGTGCAGAGT 
TTTCGAGCAC TCAGTTGGAA GCTACTCCTT GGCTATTTGG GGCCTAGGCG CAGTAGTTGG 
ACCACAACCT TGGCCCAAAA GCGAGCCCTG TATAAACAGT TCATCGAGGA GCTAGTCCTG 
CCCCCCGGAC ACTCGAGCAA TAGAGCTAGC GTAGACGGAG GAGATGGGGA CAAAGTCGAT 
TCGGGGGGCG TTGGCCTGCA AGATCATCCG CTGAGCGAGG GACCCGAAAG CGCGTGGAAC 
ACGTTCTTCA ATGATAACGA GTTTCTACTG CAAATCGACA AGGATGTCCG CCGCTTGTGC 
CCGGACATAT CCTTTTTCCA GCAGCCCACC GATTATCCCT GCGAGATTGT GGTCCACAGC 
AAAGGAGAGC ATGGACGAAG GCTCCACGAA CGGGTGGTTC CCGCCGTTCT AAGTTCGGCG 
AATGTGGAAC GCAAGGGCCT CGGCATGACA AAGATAAATT TGATTACAAA ACGCTCCGTT 
GAAAACTATG CCGCCATGGA GGAGGGCCAA GAGGCTCACT GGGAAGTTGT GCAACGCATT 
TTGTTCATCT ATGCCAAACT AAATCCCGGC CAGGGATATG TCCAGGGCAT GAACGAAATC 
GTGGGACCCA TTTACTATGT TATGGCCTCC GATCCTGACC TCACATATCG CGCCCACGCT 
GAGGCTGATT GTTTCTTTTG TTTTACCGCC CTGATGAGCG AGATAAGAGA CTTTTTTATT 
AAAACACTAG ACGACGCAGA AGGCGGCATC AAGTTCATGA TGGCCAGGCT ATCGAATATG 
CTGAAATCAA AGGACCTAAG CATTTACGAG CTACTCAGGA GCCAAGAGCT GCATCCGCAG 
TATTACAGCT TCAGGTGGCT CACACTGCTC CTGTCGCAGG AGTTTCCACT GCCAGATGTA 
CTGCGCATTT GGGATTCCGT GTTTGCGGAC GAACAGCGAT TTGATTTTCT GATAAAAATA 
TGCTGTTCAA TGATATTAAT CCAAAGGGAA GCAATTTTGG AAAACGACTT TGCCTCGAAT 
GTGAAACTCT TGCAAAACTA TCCGCCAATT GACATTAATG TTGTGATTGC TCATGCCGGA 
TCATTGGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GAPsec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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