Hn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hn
Entrez Gene ID	38871
Full Name	Henna
Synonyms	CG7399,DTPH,DTPHu,Dmel\CG7399,PAH,Pah,TPH,TRH,Tph,Trh,bu,pah
General protein information	Preferred Names Henna Names CG7399 gene product from transcript CG7399-RA CG7399-PA CG7399-PB CG7399-PC CG7399-PD Hn-PA Hn-PB Hn-PC Hn-PD Phenylalanine-hydroxylase brunette henna phenylalanine hydroxylase phenylalanine-tryptophan hydroxylase tryptophan hydrolase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66A12-66A12\|3-23.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274614.1	NP_001261543.1	henna, isoform D
NM_001274613.1	NP_001261542.1	henna, isoform C
NM_079239.4	NP_523963.2	henna, isoform A
NM_001014573.2	NP_001014573.1	henna, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PAH	XP_001156919.1
Rattus norvegicus (Norway rat)	Pah	NP_036751.2
Danio rerio (zebrafish)	pah	NP_956845.1
Caenorhabditis elegans (roundworm)	pah-1	NP_001254185.1
Xenopus tropicalis (tropical clawed frog)	pah	NP_001027484.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005712	XP_315722.4
Bos taurus (cattle)	PAH	NP_001039523.1
Mus musculus (house mouse)	Pah	NP_032803.2
Macaca mulatta (Rhesus monkey)	PAH	XP_001094859.1
Canis lupus familiaris (dog)	PAH	XP_532671.3
Homo sapiens (human)	PAH	NP_000268.1
Gallus gallus (chicken)	PAH	NP_001001298.1
Drosophila melanogaster (fruit fly)	Hn	NP_001261542.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17831	NM_001274613.1 Latest version!	Drosophila melanogaster henna, transcript variant C (Hn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa17831	NM_079239.4 Latest version!	Drosophila melanogaster henna, transcript variant A (Hn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa17832	NM_001014573.2 Latest version!	Drosophila melanogaster henna, transcript variant B (Hn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa20683	NM_001274614.1 Latest version!	Drosophila melanogaster henna, transcript variant D (Hn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hn ( NM_001274613.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261542.1 Drosophila melanogaster henna, transcript variant C (Hn), mRNA.

Price & Availability↑

CloneID	OFa17831
Clone ID Related Accession (Same CDS sequence)	NM_001274613.1 , NM_079239.4
Accession Version	NM_001274613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	henna, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACCAGC GGCAGGTCTC CTTTGATAAG CCAACGCGCG TAGAGGATTC GGCTTACATA 
GTCGAGGGTG TAGATATTAA GGAGGCCAGG AACACCTGTC TTCTGTTTTC GCCCAAGGAT 
TCGTCGTTGT CCAGCGGAGC TTTGGCCAAC ATTTTGGCCA TCTTCAAGAA GCATGACATA 
AACCTGGTGC ATATTGAGTC GCGTTCCTCG CTGCGTGTTC CCGGTTATGA GTTTTTCGTG 
GAGGCCGATG GAAAATCGGG AGCCTTGGGA AAAGCCATCG AGGATGTGAA GGAGCAGTGT 
AGCTACTTTA ACATCATTTC GCGTGACTAC AAGGATAATG CCACGGCTGT GCCCTGGTTC 
CCGCGGCGTA TACGCGATCT GGATCGTTTC GCCAATCAGA TTCTGAGCTA CGGATCGGAG 
CTGGATGCCG ATCATCCTGG TTTTACCGAT CCGGAATACC GCAAGCGTCG CAAGTACTTC 
GCCGACATTG CCTACAACTA CAAGCATGGC GAGCCCTTGC CCCATGTGGA TTACACCAAG 
GAGGAGATCG AGACCTGGGG CATTATCTTC AGGAATCTTA CGAAGCTGTA TAAGACACAT 
GCCTGTCGCG AGTACAACCA CGTCTTTCCC TTGCTGGTGG ACAACTGTGG ATTCCGAGAG 
GATAACATTC CCCAGCTGGA GGATGTGTCC AACTTTTTGA GGGATTGCAC AGGTTTCACT 
CTGCGTCCAG TGGCTGGACT ACTCAGCTCT CGGGATTTCC TGGCTGGACT GGCCTTCCGC 
GTCTTCCACT CCACTCAGTA TATCCGACAT CCCAGCAAGC CCATGTACAC ACCGGAACCA 
GATGTCTGCC ACGAGCTGAT GGGCCACGTG CCGCTTTTTG CAGATCCAGC TTTTGCCCAA 
TTCAGTCAGG AGATCGGGCT AGCTTCCCTG GGAGCACCCG ATGATTACAT TGAGAAATTG 
TCCACCATCT TCTGGTTCAC TGTTGAGTAC GGCGTGTGCC GCCAGGAGGG TGAGTTGAAG 
GCGTACGGAG CTGGTCTGCT GTCCTCCTAC GGGGAACTGG AGTATTGCCT CACGGACAAG 
CCGCAGCTGA AGGACTTTGA GCCGGAGGTC ACTGGCGTCA CCAAGTATCC CATTACTCAG 
TTCCAGCCTC TGTACTATGT GGCCGATAGT TTCGAAACCG CCAAGGAGAA GACCATTAAA 
TTTGCGAACT CGATTCCCCG ACCCTTTGGT GTTCGCTACA ATGCCTATAC TCAGAGTGTT 
GAGGTTCTCG ACTCGAAGCC CCAGATTTCG AATCTGATGG ACAACATTAA CTCCGAGTTC 
CAGATTCTTC AGAATGCCGT TGCCAAGCTG CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261542.1
CDS	77..1435
Translation	MYQRQVSFDKPTRVEDSAYIVEGVDIKEARNTCLLFSPKDSSLSSGALANILAIFKKHDINLVHIESRSSLRVPGYEFFVEADGKSGALGKAIEDVKEQCSYFNIISRDYKDNATAVPWFPRRIRDLDRFANQILSYGSELDADHPGFTDPEYRKRRKYFADIAYNYKHGEPLPHVDYTKEEIETWGIIFRNLTKLYKTHACREYNHVFPLLVDNCGFREDNIPQLEDVSNFLRDCTGFTLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHPSKPMYTPEPDVCHELMGHVPLFADPAFAQFSQEIGLASLGAPDDYIEKLSTIFWFTVEYGVCRQEGELKAYGAGLLSSYGELEYCLTDKPQLKDFEPEVTGVTKYPITQFQPLYYVADSFETAKEKTIKFANSIPRPFGVRYNAYTQSVEVLDSKPQISNLMDNINSEFQILQNAVAKLRV

Target ORF information:

RefSeq Version	NM_001274613.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster henna, transcript variant C (Hn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274613.1

ORF Insert Sequence:

ATGTACCAGC GGCAGGTCTC CTTTGATAAG CCAACGCGCG TAGAGGATTC GGCTTACATA 
GTCGAGGGTG TAGATATTAA GGAGGCCAGG AACACCTGTC TTCTGTTTTC GCCCAAGGAT 
TCGTCGTTGT CCAGCGGAGC TTTGGCCAAC ATTTTGGCCA TCTTCAAGAA GCATGACATA 
AACCTGGTGC ATATTGAGTC GCGTTCCTCG CTGCGTGTTC CCGGTTATGA GTTTTTCGTG 
GAGGCCGATG GAAAATCGGG AGCCTTGGGA AAAGCCATCG AGGATGTGAA GGAGCAGTGT 
AGCTACTTTA ACATCATTTC GCGTGACTAC AAGGATAATG CCACGGCTGT GCCCTGGTTC 
CCGCGGCGTA TACGCGATCT GGATCGTTTC GCCAATCAGA TTCTGAGCTA CGGATCGGAG 
CTGGATGCCG ATCATCCTGG TTTTACCGAT CCGGAATACC GCAAGCGTCG CAAGTACTTC 
GCCGACATTG CCTACAACTA CAAGCATGGC GAGCCCTTGC CCCATGTGGA TTACACCAAG 
GAGGAGATCG AGACCTGGGG CATTATCTTC AGGAATCTTA CGAAGCTGTA TAAGACACAT 
GCCTGTCGCG AGTACAACCA CGTCTTTCCC TTGCTGGTGG ACAACTGTGG ATTCCGAGAG 
GATAACATTC CCCAGCTGGA GGATGTGTCC AACTTTTTGA GGGATTGCAC AGGTTTCACT 
CTGCGTCCAG TGGCTGGACT ACTCAGCTCT CGGGATTTCC TGGCTGGACT GGCCTTCCGC 
GTCTTCCACT CCACTCAGTA TATCCGACAT CCCAGCAAGC CCATGTACAC ACCGGAACCA 
GATGTCTGCC ACGAGCTGAT GGGCCACGTG CCGCTTTTTG CAGATCCAGC TTTTGCCCAA 
TTCAGTCAGG AGATCGGGCT AGCTTCCCTG GGAGCACCCG ATGATTACAT TGAGAAATTG 
TCCACCATCT TCTGGTTCAC TGTTGAGTAC GGCGTGTGCC GCCAGGAGGG TGAGTTGAAG 
GCGTACGGAG CTGGTCTGCT GTCCTCCTAC GGGGAACTGG AGTATTGCCT CACGGACAAG 
CCGCAGCTGA AGGACTTTGA GCCGGAGGTC ACTGGCGTCA CCAAGTATCC CATTACTCAG 
TTCCAGCCTC TGTACTATGT GGCCGATAGT TTCGAAACCG CCAAGGAGAA GACCATTAAA 
TTTGCGAACT CGATTCCCCG ACCCTTTGGT GTTCGCTACA ATGCCTATAC TCAGAGTGTT 
GAGGTTCTCG ACTCGAAGCC CCAGATTTCG AATCTGATGG ACAACATTAA CTCCGAGTTC 
CAGATTCTTC AGAATGCCGT TGCCAAGCTG CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hn ( NM_079239.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523963.2 Drosophila melanogaster henna, transcript variant A (Hn), mRNA.

Price & Availability↑

CloneID	OFa17831
Clone ID Related Accession (Same CDS sequence)	NM_001274613.1 , NM_079239.4
Accession Version	NM_079239.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	henna, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079239.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACCAGC GGCAGGTCTC CTTTGATAAG CCAACGCGCG TAGAGGATTC GGCTTACATA 
GTCGAGGGTG TAGATATTAA GGAGGCCAGG AACACCTGTC TTCTGTTTTC GCCCAAGGAT 
TCGTCGTTGT CCAGCGGAGC TTTGGCCAAC ATTTTGGCCA TCTTCAAGAA GCATGACATA 
AACCTGGTGC ATATTGAGTC GCGTTCCTCG CTGCGTGTTC CCGGTTATGA GTTTTTCGTG 
GAGGCCGATG GAAAATCGGG AGCCTTGGGA AAAGCCATCG AGGATGTGAA GGAGCAGTGT 
AGCTACTTTA ACATCATTTC GCGTGACTAC AAGGATAATG CCACGGCTGT GCCCTGGTTC 
CCGCGGCGTA TACGCGATCT GGATCGTTTC GCCAATCAGA TTCTGAGCTA CGGATCGGAG 
CTGGATGCCG ATCATCCTGG TTTTACCGAT CCGGAATACC GCAAGCGTCG CAAGTACTTC 
GCCGACATTG CCTACAACTA CAAGCATGGC GAGCCCTTGC CCCATGTGGA TTACACCAAG 
GAGGAGATCG AGACCTGGGG CATTATCTTC AGGAATCTTA CGAAGCTGTA TAAGACACAT 
GCCTGTCGCG AGTACAACCA CGTCTTTCCC TTGCTGGTGG ACAACTGTGG ATTCCGAGAG 
GATAACATTC CCCAGCTGGA GGATGTGTCC AACTTTTTGA GGGATTGCAC AGGTTTCACT 
CTGCGTCCAG TGGCTGGACT ACTCAGCTCT CGGGATTTCC TGGCTGGACT GGCCTTCCGC 
GTCTTCCACT CCACTCAGTA TATCCGACAT CCCAGCAAGC CCATGTACAC ACCGGAACCA 
GATGTCTGCC ACGAGCTGAT GGGCCACGTG CCGCTTTTTG CAGATCCAGC TTTTGCCCAA 
TTCAGTCAGG AGATCGGGCT AGCTTCCCTG GGAGCACCCG ATGATTACAT TGAGAAATTG 
TCCACCATCT TCTGGTTCAC TGTTGAGTAC GGCGTGTGCC GCCAGGAGGG TGAGTTGAAG 
GCGTACGGAG CTGGTCTGCT GTCCTCCTAC GGGGAACTGG AGTATTGCCT CACGGACAAG 
CCGCAGCTGA AGGACTTTGA GCCGGAGGTC ACTGGCGTCA CCAAGTATCC CATTACTCAG 
TTCCAGCCTC TGTACTATGT GGCCGATAGT TTCGAAACCG CCAAGGAGAA GACCATTAAA 
TTTGCGAACT CGATTCCCCG ACCCTTTGGT GTTCGCTACA ATGCCTATAC TCAGAGTGTT 
GAGGTTCTCG ACTCGAAGCC CCAGATTTCG AATCTGATGG ACAACATTAA CTCCGAGTTC 
CAGATTCTTC AGAATGCCGT TGCCAAGCTG CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523963.2
CDS	175..1533
Translation	MYQRQVSFDKPTRVEDSAYIVEGVDIKEARNTCLLFSPKDSSLSSGALANILAIFKKHDINLVHIESRSSLRVPGYEFFVEADGKSGALGKAIEDVKEQCSYFNIISRDYKDNATAVPWFPRRIRDLDRFANQILSYGSELDADHPGFTDPEYRKRRKYFADIAYNYKHGEPLPHVDYTKEEIETWGIIFRNLTKLYKTHACREYNHVFPLLVDNCGFREDNIPQLEDVSNFLRDCTGFTLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHPSKPMYTPEPDVCHELMGHVPLFADPAFAQFSQEIGLASLGAPDDYIEKLSTIFWFTVEYGVCRQEGELKAYGAGLLSSYGELEYCLTDKPQLKDFEPEVTGVTKYPITQFQPLYYVADSFETAKEKTIKFANSIPRPFGVRYNAYTQSVEVLDSKPQISNLMDNINSEFQILQNAVAKLRV

Target ORF information:

RefSeq Version	NM_079239.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster henna, transcript variant A (Hn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079239.4

ORF Insert Sequence:

ATGTACCAGC GGCAGGTCTC CTTTGATAAG CCAACGCGCG TAGAGGATTC GGCTTACATA 
GTCGAGGGTG TAGATATTAA GGAGGCCAGG AACACCTGTC TTCTGTTTTC GCCCAAGGAT 
TCGTCGTTGT CCAGCGGAGC TTTGGCCAAC ATTTTGGCCA TCTTCAAGAA GCATGACATA 
AACCTGGTGC ATATTGAGTC GCGTTCCTCG CTGCGTGTTC CCGGTTATGA GTTTTTCGTG 
GAGGCCGATG GAAAATCGGG AGCCTTGGGA AAAGCCATCG AGGATGTGAA GGAGCAGTGT 
AGCTACTTTA ACATCATTTC GCGTGACTAC AAGGATAATG CCACGGCTGT GCCCTGGTTC 
CCGCGGCGTA TACGCGATCT GGATCGTTTC GCCAATCAGA TTCTGAGCTA CGGATCGGAG 
CTGGATGCCG ATCATCCTGG TTTTACCGAT CCGGAATACC GCAAGCGTCG CAAGTACTTC 
GCCGACATTG CCTACAACTA CAAGCATGGC GAGCCCTTGC CCCATGTGGA TTACACCAAG 
GAGGAGATCG AGACCTGGGG CATTATCTTC AGGAATCTTA CGAAGCTGTA TAAGACACAT 
GCCTGTCGCG AGTACAACCA CGTCTTTCCC TTGCTGGTGG ACAACTGTGG ATTCCGAGAG 
GATAACATTC CCCAGCTGGA GGATGTGTCC AACTTTTTGA GGGATTGCAC AGGTTTCACT 
CTGCGTCCAG TGGCTGGACT ACTCAGCTCT CGGGATTTCC TGGCTGGACT GGCCTTCCGC 
GTCTTCCACT CCACTCAGTA TATCCGACAT CCCAGCAAGC CCATGTACAC ACCGGAACCA 
GATGTCTGCC ACGAGCTGAT GGGCCACGTG CCGCTTTTTG CAGATCCAGC TTTTGCCCAA 
TTCAGTCAGG AGATCGGGCT AGCTTCCCTG GGAGCACCCG ATGATTACAT TGAGAAATTG 
TCCACCATCT TCTGGTTCAC TGTTGAGTAC GGCGTGTGCC GCCAGGAGGG TGAGTTGAAG 
GCGTACGGAG CTGGTCTGCT GTCCTCCTAC GGGGAACTGG AGTATTGCCT CACGGACAAG 
CCGCAGCTGA AGGACTTTGA GCCGGAGGTC ACTGGCGTCA CCAAGTATCC CATTACTCAG 
TTCCAGCCTC TGTACTATGT GGCCGATAGT TTCGAAACCG CCAAGGAGAA GACCATTAAA 
TTTGCGAACT CGATTCCCCG ACCCTTTGGT GTTCGCTACA ATGCCTATAC TCAGAGTGTT 
GAGGTTCTCG ACTCGAAGCC CCAGATTTCG AATCTGATGG ACAACATTAA CTCCGAGTTC 
CAGATTCTTC AGAATGCCGT TGCCAAGCTG CGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hn ( NM_001014573.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014573.1 Drosophila melanogaster henna, transcript variant B (Hn), mRNA.

Price & Availability↑

CloneID	OFa17832
Clone ID Related Accession (Same CDS sequence)	NM_001014573.2
Accession Version	NM_001014573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	henna, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014573.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATTTAT TACAGCCAAC GCGCGTAGAG GATTCGGCTT ACATAGTCGA GGGTGTAGAT 
ATTAAGGAGG CCAGGAACAC CTGTCTTCTG TTTTCGCCCA AGGATTCGTC GTTGTCCAGC 
GGAGCTTTGG CCAACATTTT GGCCATCTTC AAGAAGCATG ACATAAACCT GGTGCATATT 
GAGTCGCGTT CCTCGCTGCG TGTTCCCGGT TATGAGTTTT TCGTGGAGGC CGATGGAAAA 
TCGGGAGCCT TGGGAAAAGC CATCGAGGAT GTGAAGGAGC AGTGTAGCTA CTTTAACATC 
ATTTCGCGTG ACTACAAGGA TAATGCCACG GCTGTGCCCT GGTTCCCGCG GCGTATACGC 
GATCTGGATC GTTTCGCCAA TCAGATTCTG AGCTACGGAT CGGAGCTGGA TGCCGATCAT 
CCTGGTTTTA CCGATCCGGA ATACCGCAAG CGTCGCAAGT ACTTCGCCGA CATTGCCTAC 
AACTACAAGC ATGGCGAGCC CTTGCCCCAT GTGGATTACA CCAAGGAGGA GATCGAGACC 
TGGGGCATTA TCTTCAGGAA TCTTACGAAG CTGTATAAGA CACATGCCTG TCGCGAGTAC 
AACCACGTCT TTCCCTTGCT GGTGGACAAC TGTGGATTCC GAGAGGATAA CATTCCCCAG 
CTGGAGGATG TGTCCAACTT TTTGAGGGAT TGCACAGGTT TCACTCTGCG TCCAGTGGCT 
GGACTACTCA GCTCTCGGGA TTTCCTGGCT GGACTGGCCT TCCGCGTCTT CCACTCCACT 
CAGTATATCC GACATCCCAG CAAGCCCATG TACACACCGG AACCAGATGT CTGCCACGAG 
CTGATGGGCC ACGTGCCGCT TTTTGCAGAT CCAGCTTTTG CCCAATTCAG TCAGGAGATC 
GGGCTAGCTT CCCTGGGAGC ACCCGATGAT TACATTGAGA AATTGTCCAC CATCTTCTGG 
TTCACTGTTG AGTACGGCGT GTGCCGCCAG GAGGGTGAGT TGAAGGCGTA CGGAGCTGGT 
CTGCTGTCCT CCTACGGGGA ACTGGAGTAT TGCCTCACGG ACAAGCCGCA GCTGAAGGAC 
TTTGAGCCGG AGGTCACTGG CGTCACCAAG TATCCCATTA CTCAGTTCCA GCCTCTGTAC 
TATGTGGCCG ATAGTTTCGA AACCGCCAAG GAGAAGACCA TTAAATTTGC GAACTCGATT 
CCCCGACCCT TTGGTGTTCG CTACAATGCC TATACTCAGA GTGTTGAGGT TCTCGACTCG 
AAGCCCCAGA TTTCGAATCT GATGGACAAC ATTAACTCCG AGTTCCAGAT TCTTCAGAAT 
GCCGTTGCCA AGCTGCGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014573.1
CDS	182..1525
Translation	MNLLQPTRVEDSAYIVEGVDIKEARNTCLLFSPKDSSLSSGALANILAIFKKHDINLVHIESRSSLRVPGYEFFVEADGKSGALGKAIEDVKEQCSYFNIISRDYKDNATAVPWFPRRIRDLDRFANQILSYGSELDADHPGFTDPEYRKRRKYFADIAYNYKHGEPLPHVDYTKEEIETWGIIFRNLTKLYKTHACREYNHVFPLLVDNCGFREDNIPQLEDVSNFLRDCTGFTLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHPSKPMYTPEPDVCHELMGHVPLFADPAFAQFSQEIGLASLGAPDDYIEKLSTIFWFTVEYGVCRQEGELKAYGAGLLSSYGELEYCLTDKPQLKDFEPEVTGVTKYPITQFQPLYYVADSFETAKEKTIKFANSIPRPFGVRYNAYTQSVEVLDSKPQISNLMDNINSEFQILQNAVAKLRV

Target ORF information:

RefSeq Version	NM_001014573.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster henna, transcript variant B (Hn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014573.2

ORF Insert Sequence:

ATGAATTTAT TACAGCCAAC GCGCGTAGAG GATTCGGCTT ACATAGTCGA GGGTGTAGAT 
ATTAAGGAGG CCAGGAACAC CTGTCTTCTG TTTTCGCCCA AGGATTCGTC GTTGTCCAGC 
GGAGCTTTGG CCAACATTTT GGCCATCTTC AAGAAGCATG ACATAAACCT GGTGCATATT 
GAGTCGCGTT CCTCGCTGCG TGTTCCCGGT TATGAGTTTT TCGTGGAGGC CGATGGAAAA 
TCGGGAGCCT TGGGAAAAGC CATCGAGGAT GTGAAGGAGC AGTGTAGCTA CTTTAACATC 
ATTTCGCGTG ACTACAAGGA TAATGCCACG GCTGTGCCCT GGTTCCCGCG GCGTATACGC 
GATCTGGATC GTTTCGCCAA TCAGATTCTG AGCTACGGAT CGGAGCTGGA TGCCGATCAT 
CCTGGTTTTA CCGATCCGGA ATACCGCAAG CGTCGCAAGT ACTTCGCCGA CATTGCCTAC 
AACTACAAGC ATGGCGAGCC CTTGCCCCAT GTGGATTACA CCAAGGAGGA GATCGAGACC 
TGGGGCATTA TCTTCAGGAA TCTTACGAAG CTGTATAAGA CACATGCCTG TCGCGAGTAC 
AACCACGTCT TTCCCTTGCT GGTGGACAAC TGTGGATTCC GAGAGGATAA CATTCCCCAG 
CTGGAGGATG TGTCCAACTT TTTGAGGGAT TGCACAGGTT TCACTCTGCG TCCAGTGGCT 
GGACTACTCA GCTCTCGGGA TTTCCTGGCT GGACTGGCCT TCCGCGTCTT CCACTCCACT 
CAGTATATCC GACATCCCAG CAAGCCCATG TACACACCGG AACCAGATGT CTGCCACGAG 
CTGATGGGCC ACGTGCCGCT TTTTGCAGAT CCAGCTTTTG CCCAATTCAG TCAGGAGATC 
GGGCTAGCTT CCCTGGGAGC ACCCGATGAT TACATTGAGA AATTGTCCAC CATCTTCTGG 
TTCACTGTTG AGTACGGCGT GTGCCGCCAG GAGGGTGAGT TGAAGGCGTA CGGAGCTGGT 
CTGCTGTCCT CCTACGGGGA ACTGGAGTAT TGCCTCACGG ACAAGCCGCA GCTGAAGGAC 
TTTGAGCCGG AGGTCACTGG CGTCACCAAG TATCCCATTA CTCAGTTCCA GCCTCTGTAC 
TATGTGGCCG ATAGTTTCGA AACCGCCAAG GAGAAGACCA TTAAATTTGC GAACTCGATT 
CCCCGACCCT TTGGTGTTCG CTACAATGCC TATACTCAGA GTGTTGAGGT TCTCGACTCG 
AAGCCCCAGA TTTCGAATCT GATGGACAAC ATTAACTCCG AGTTCCAGAT TCTTCAGAAT 
GCCGTTGCCA AGCTGCGCGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hn ( NM_001274614.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261543.1 Drosophila melanogaster henna, transcript variant D (Hn), mRNA.

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CloneID	OFa20683
Clone ID Related Accession (Same CDS sequence)	NM_001274614.1
Accession Version	NM_001274614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	henna, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACACAC CGGAACCAGA TGTCTGCCAC GAGCTGATGG GCCACGTGCC GCTTTTTGCA 
GATCCAGCTT TTGCCCAATT CAGTCAGGAG ATCGGGCTAG CTTCCCTGGG AGCACCCGAT 
GATTACATTG AGAAATTGTC CACCATCTTC TGGTTCACTG TTGAGTACGG CGTGTGCCGC 
CAGGAGGGTG AGTTGAAGGC GTACGGAGCT GGTCTGCTGT CCTCCTACGG GGAACTGGAG 
TATTGCCTCA CGGACAAGCC GCAGCTGAAG GACTTTGAGC CGGAGGTCAC TGGCGTCACC 
AAGTATCCCA TTACTCAGTT CCAGCCTCTG TACTATGTGG CCGATAGTTT CGAAACCGCC 
AAGGAGAAGA CCATTAAATT TGCGAACTCG ATTCCCCGAC CCTTTGGTGT TCGCTACAAT 
GCCTATACTC AGAGTGTTGA GGTTCTCGAC TCGAAGCCCC AGATTTCGAA TCTGATGGAC 
AACATTAACT CCGAGTTCCA GATTCTTCAG AATGCCGTTG CCAAGCTGCG CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261543.1
CDS	1193..1729
Translation	MYTPEPDVCHELMGHVPLFADPAFAQFSQEIGLASLGAPDDYIEKLSTIFWFTVEYGVCRQEGELKAYGAGLLSSYGELEYCLTDKPQLKDFEPEVTGVTKYPITQFQPLYYVADSFETAKEKTIKFANSIPRPFGVRYNAYTQSVEVLDSKPQISNLMDNINSEFQILQNAVAKLRV

Target ORF information:

RefSeq Version	NM_001274614.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster henna, transcript variant D (Hn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274614.1

ORF Insert Sequence:

ATGTACACAC CGGAACCAGA TGTCTGCCAC GAGCTGATGG GCCACGTGCC GCTTTTTGCA 
GATCCAGCTT TTGCCCAATT CAGTCAGGAG ATCGGGCTAG CTTCCCTGGG AGCACCCGAT 
GATTACATTG AGAAATTGTC CACCATCTTC TGGTTCACTG TTGAGTACGG CGTGTGCCGC 
CAGGAGGGTG AGTTGAAGGC GTACGGAGCT GGTCTGCTGT CCTCCTACGG GGAACTGGAG 
TATTGCCTCA CGGACAAGCC GCAGCTGAAG GACTTTGAGC CGGAGGTCAC TGGCGTCACC 
AAGTATCCCA TTACTCAGTT CCAGCCTCTG TACTATGTGG CCGATAGTTT CGAAACCGCC 
AAGGAGAAGA CCATTAAATT TGCGAACTCG ATTCCCCGAC CCTTTGGTGT TCGCTACAAT 
GCCTATACTC AGAGTGTTGA GGTTCTCGAC TCGAAGCCCC AGATTTCGAA TCTGATGGAC 
AACATTAACT CCGAGTTCCA GATTCTTCAG AATGCCGTTG CCAAGCTGCG CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hn cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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