GluRIA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GluRIA
Entrez Gene ID	38742
Full Name	Glutamate receptor IA
Synonyms	CG8442,DGluR-I,DGluR-IA,DGluRI,DmelGlu-R1,Dmel\CG8442,Dro GluRIA,Glu-R1,Glu-RI,GluR1,GluRI,dGluRI,dglur-IA
General protein information	Preferred Names Glutamate receptor IA Names CG8442 gene product from transcript CG8442-RA CG8442-PA GluRIA-PA glutamate receptor glutamate receptor I glutamate receptor IA glutamate receptor kainate-selective subunit kainate receptor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65C1-65C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057507.4	NP_476855.1	glutamate receptor IA

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRIA3	XP_003317712.1
Danio rerio (zebrafish)	gria3b	NP_938174.1
Bos taurus (cattle)	GRIA3	NP_001192988.1
Gallus gallus (chicken)	GRIA3	NP_990546.2
Xenopus tropicalis (tropical clawed frog)	gria3	NP_001106494.1
Canis lupus familiaris (dog)	GRIA3	XP_005641824.1
Drosophila melanogaster (fruit fly)	Glu-RI	NP_476855.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006027	XP_316066.4
Homo sapiens (human)	GRIA3	NP_015564.4
Macaca mulatta (Rhesus monkey)	GRIA3	XP_001088327.1
Drosophila melanogaster (fruit fly)	Glu-RIB	NP_001261622.1
Caenorhabditis elegans (roundworm)	glr-2	NP_001021114.1
Mus musculus (house mouse)	Gria3	NP_001268858.1
Rattus norvegicus (Norway rat)	Gria3	NP_116785.2
Danio rerio (zebrafish)	gria3a	NP_938153.1
Caenorhabditis elegans (roundworm)	glr-1	NP_498887.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GluRIA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GluRIA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17711	NM_057507.4 Latest version!	Drosophila melanogaster glutamate receptor IA (GluRIA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GluRIA ( NM_057507.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476855.1 Drosophila melanogaster glutamate receptor IA (GluRIA), mRNA.

Price & Availability↑

CloneID	OFa17711
Clone ID Related Accession (Same CDS sequence)	NM_057507.4
Accession Version	NM_057507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	glutamate receptor IA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057507.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTCGA GGCTAAAATT CTTGGCATAT TTACATTTTA TATGCGCCAG CAGCATTTTC 
TGGCCGGAAT TTAGTTCAGC CCAGCAGCAG CAACAGACGG TATCGTTAAC CGAGAAAATA 
CCATTAGGTG CAATCTTCGA ACAGGGAACG GATGATGTGC AATCAGCCTT TAAATATGCA 
ATGCTCAATC ACAATCTGAA TGTGAGCTCT CGACGCTTCG AATTGCAGGC GTATGTCGAT 
GTCATCAATA CAGCGGATGC ATTCAAATTA TCCCGCCTGA TTTGCAATCA ATTCTCGCGC 
GGAGTCTACT CAATGCTGGG CGCCGTATCG CCGGACTCGT TTGACACATT GCACTCGTAC 
TCGAATACGT TCCAAATGCC ATTTGTGACG CCCTGGTTTC CCGAGAAGGT GCTTGCGCCG 
TCGTCGGGTC TCCTGGATTT CGCTATCAGC ATGCGTCCGG ATTATCATCA GGCGATTATC 
GATACCATCC AGTACTACGG CTGGCAGAGC ATTATTTATC TCTACGACTC GCACGATGGC 
TTACTACGGC TGCAGCAAAT CTATCAAGAA CTGAAGCCCG GAAATGAAAC GTTCCGTGTG 
CAAATGGTGA AACGCATCGC CAACGTAACG ATGGCCATTG AATTTTTGCA CACGCTGGAG 
GATTTGGGTC GCTTTAGCAA AAAGCGCATC GTGCTCGACT GCCCGGCGGA AATGGCCAAA 
GAGATCATCG TCCAGCATGT GCGGGACATC AAGCTGGGCC GGCGCACCTA TCATTACCTG 
CTCAGCGGAC TGGTCATGGA CAACCACTGG CCCAGCGATG TGGTCGAGTT TGGGGCCATC 
AACATCACCG GCTTTCGGAT TGTCGACTCT AATCGGAGGG CGGTGCGTGA TTTCCACGAC 
AGCCGGAAAC GTTTGGAGCC GAGCGGCCAA AGCCAAAGCC AGAACGCAGG GGGGCCCAAC 
AGCTTGCCGG CCATTTCGGC CCAGGCTGCA CTGATGTACG ATGCGGTTTT TGTGCTCGTC 
GAGGCCTTCA ATCGCATTTT GCGCAAGAAG CCGGATCAGT TCCGTTCCAA TCATCTGCAG 
CGCCGCAGCC ACGGAGGCAG CTCCTCCTCA TCCGCCACGG GCACCAATGA GTCCAGTGCC 
CTCCTCGATT GCAACACCAG CAAGGGCTGG GTCACGCCAT GGGAGCAGGG CGAAAAAATA 
TCAAGGGTGC TGAGAAAGGT GGAAATCGAC GGTTTGTCGG GCGAGATCCG TTTCGACGAG 
GACGGACGTC GCATCAACTA CACGCTGCAT GTGGTGGAGA TGTCCGTGAA CAGCACCTTG 
CAGCAGGTGG CCGAGTGGCG TGATGACGCC GGACTCCTTC CGCTCCACAG CCATAACTAC 
GCGTCGTCAT CGCGTTCCGC GTCGGCCAGC ACCGGAGATT ACGACCGGAA TCACACGTAC 
ATCGTGAGTA GTCTGCTGGA GGAGCCGTAT CTGTCGCTCA AGCAGTACAC CTACGGAGAA 
TCATTGGTGG GCAACGATCG ATTCGAGGGA TACTGCAAGG ATTTGGCCGA TATGCTAGCA 
GCTCAACTGG GAATTAAATA TGAAATACGC CTGGTGCAAG ATGGCAACTA TGGTGCAGAG 
AATCAGTATG CTCCTGGCGG TTGGGATGGC ATGGTGGGTG AGCTGATTCG CAAGGAGGCG 
GATATAGCCA TATCCGCAAT GACTATTACA GCCGAAAGAG AACGGGTTAT AGACTTTAGC 
AAACCCTTTA TGACCCTGGG AATATCCATT ATGATAAAGA AGCCCGTGAA ACAGACACCA 
GGAGTGTTTA GTTTTCTTAA TCCCCTATCC CAGGAAATTT GGATAAGCGT GATTCTCAGC 
TATGTGGGTG TTAGTTTTGT TCTGTACTTT GTTACACGTT TCCCACCCTA TGAGTGGCGG 
ATTGTGAGGC GTCCTCAAGC GGATTCCACT GCCCAGCAAC CACCGGGAAT TATTGGAGGT 
GCCACTTTGA GTGAACCACA AGCGCATGTG CCTCCAGTTC CACCTAATGA GTTCACTATG 
CTGAACTCCT TTTGGTATTC CTTGGCTGCT TTTATGCAGC AGGGATGCGA TATTACACCT 
CCTTCGATTG CCGGAAGGAT AGCAGCTGCC GTCTGGTGGT TCTTCACTAT CATATTAATA 
TCCTCGTATA CCGCCAATTT GGCCGCTTTT CTCACAGTGG AGCGTATGGT GGCGCCCATC 
AAAACGCCCG AGGACTTGAC CATGCAAACG GATGTGAACT ATGGTACCCT GTTATACGGC 
TCCACTTGGG AATTCTTTAG GCGTTCACAA ATCGGTCTGC ACAATAAAAT GTGGGAGTAT 
ATGAATGCCA ATCAACACCA TTCGGTGCAC ACCTATGATG AGGGTATTCG TAGGGTGAGG 
CAATCCAAGG GCAAGTATGC CCTACTGGTG GAGTCACCCA AAAACGAGTA TGTAAATGCT 
AGGCCTCCGT GCGACACAAT GAAAGTGGGA CGCAATATCG ATACGAAGGG ATTTGGCGTG 
GCCACACCCA TAGGATCGCC ACTGAGAAAA CGCTTAAACG AGGCCGTGCT AACGCTAAAG 
GAGAACGGTG AGCTGTTGCG GATTAGGAAT AAATGGTGGT TCGACAAGAC CGAGTGCAAT 
CTGGACCAGG AGACCTCCAC ACCGAACGAG CTCTCGCTAA GCAATGTGGC CGGGATCTAC 
TACATCCTAA TCGGAGGCCT GCTCCTGGCC GTGATTGTGG CCATCATGGA GTTCTTCTGC 
CGCAACAAAA CCCCGCAGCT AAAGTCGCCA GGATCCAATG GATCCGCTGG TGGAGTGCCC 
GGAATGCTGG CCTCCTCAAC ATATCAAAGG GATTCCCTTT CCGATGCGAT TATGCATTCG 
CAGGCCAAGT TGGCGATGCA GGCGAGCAGC GAGTATGACG AGCGATTGGT GGGCGTTGAG 
TTGGCCTCGA ACGTGCGGTA TCAGTACAGC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476855.1
CDS	539..3514
Translation	MHSRLKFLAYLHFICASSIFWPEFSSAQQQQQTVSLTEKIPLGAIFEQGTDDVQSAFKYAMLNHNLNVSSRRFELQAYVDVINTADAFKLSRLICNQFSRGVYSMLGAVSPDSFDTLHSYSNTFQMPFVTPWFPEKVLAPSSGLLDFAISMRPDYHQAIIDTIQYYGWQSIIYLYDSHDGLLRLQQIYQELKPGNETFRVQMVKRIANVTMAIEFLHTLEDLGRFSKKRIVLDCPAEMAKEIIVQHVRDIKLGRRTYHYLLSGLVMDNHWPSDVVEFGAINITGFRIVDSNRRAVRDFHDSRKRLEPSGQSQSQNAGGPNSLPAISAQAALMYDAVFVLVEAFNRILRKKPDQFRSNHLQRRSHGGSSSSSATGTNESSALLDCNTSKGWVTPWEQGEKISRVLRKVEIDGLSGEIRFDEDGRRINYTLHVVEMSVNSTLQQVAEWRDDAGLLPLHSHNYASSSRSASASTGDYDRNHTYIVSSLLEEPYLSLKQYTYGESLVGNDRFEGYCKDLADMLAAQLGIKYEIRLVQDGNYGAENQYAPGGWDGMVGELIRKEADIAISAMTITAERERVIDFSKPFMTLGISIMIKKPVKQTPGVFSFLNPLSQEIWISVILSYVGVSFVLYFVTRFPPYEWRIVRRPQADSTAQQPPGIIGGATLSEPQAHVPPVPPNEFTMLNSFWYSLAAFMQQGCDITPPSIAGRIAAAVWWFFTIILISSYTANLAAFLTVERMVAPIKTPEDLTMQTDVNYGTLLYGSTWEFFRRSQIGLHNKMWEYMNANQHHSVHTYDEGIRRVRQSKGKYALLVESPKNEYVNARPPCDTMKVGRNIDTKGFGVATPIGSPLRKRLNEAVLTLKENGELLRIRNKWWFDKTECNLDQETSTPNELSLSNVAGIYYILIGGLLLAVIVAIMEFFCRNKTPQLKSPGSNGSAGGVPGMLASSTYQRDSLSDAIMHSQAKLAMQASSEYDERLVGVELASNVRYQYSM

Target ORF information:

RefSeq Version	NM_057507.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glutamate receptor IA (GluRIA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057507.4

ORF Insert Sequence:

ATGCATTCGA GGCTAAAATT CTTGGCATAT TTACATTTTA TATGCGCCAG CAGCATTTTC 
TGGCCGGAAT TTAGTTCAGC CCAGCAGCAG CAACAGACGG TATCGTTAAC CGAGAAAATA 
CCATTAGGTG CAATCTTCGA ACAGGGAACG GATGATGTGC AATCAGCCTT TAAATATGCA 
ATGCTCAATC ACAATCTGAA TGTGAGCTCT CGACGCTTCG AATTGCAGGC GTATGTCGAT 
GTCATCAATA CAGCGGATGC ATTCAAATTA TCCCGCCTGA TTTGCAATCA ATTCTCGCGC 
GGAGTCTACT CAATGCTGGG CGCCGTATCG CCGGACTCGT TTGACACATT GCACTCGTAC 
TCGAATACGT TCCAAATGCC ATTTGTGACG CCCTGGTTTC CCGAGAAGGT GCTTGCGCCG 
TCGTCGGGTC TCCTGGATTT CGCTATCAGC ATGCGTCCGG ATTATCATCA GGCGATTATC 
GATACCATCC AGTACTACGG CTGGCAGAGC ATTATTTATC TCTACGACTC GCACGATGGC 
TTACTACGGC TGCAGCAAAT CTATCAAGAA CTGAAGCCCG GAAATGAAAC GTTCCGTGTG 
CAAATGGTGA AACGCATCGC CAACGTAACG ATGGCCATTG AATTTTTGCA CACGCTGGAG 
GATTTGGGTC GCTTTAGCAA AAAGCGCATC GTGCTCGACT GCCCGGCGGA AATGGCCAAA 
GAGATCATCG TCCAGCATGT GCGGGACATC AAGCTGGGCC GGCGCACCTA TCATTACCTG 
CTCAGCGGAC TGGTCATGGA CAACCACTGG CCCAGCGATG TGGTCGAGTT TGGGGCCATC 
AACATCACCG GCTTTCGGAT TGTCGACTCT AATCGGAGGG CGGTGCGTGA TTTCCACGAC 
AGCCGGAAAC GTTTGGAGCC GAGCGGCCAA AGCCAAAGCC AGAACGCAGG GGGGCCCAAC 
AGCTTGCCGG CCATTTCGGC CCAGGCTGCA CTGATGTACG ATGCGGTTTT TGTGCTCGTC 
GAGGCCTTCA ATCGCATTTT GCGCAAGAAG CCGGATCAGT TCCGTTCCAA TCATCTGCAG 
CGCCGCAGCC ACGGAGGCAG CTCCTCCTCA TCCGCCACGG GCACCAATGA GTCCAGTGCC 
CTCCTCGATT GCAACACCAG CAAGGGCTGG GTCACGCCAT GGGAGCAGGG CGAAAAAATA 
TCAAGGGTGC TGAGAAAGGT GGAAATCGAC GGTTTGTCGG GCGAGATCCG TTTCGACGAG 
GACGGACGTC GCATCAACTA CACGCTGCAT GTGGTGGAGA TGTCCGTGAA CAGCACCTTG 
CAGCAGGTGG CCGAGTGGCG TGATGACGCC GGACTCCTTC CGCTCCACAG CCATAACTAC 
GCGTCGTCAT CGCGTTCCGC GTCGGCCAGC ACCGGAGATT ACGACCGGAA TCACACGTAC 
ATCGTGAGTA GTCTGCTGGA GGAGCCGTAT CTGTCGCTCA AGCAGTACAC CTACGGAGAA 
TCATTGGTGG GCAACGATCG ATTCGAGGGA TACTGCAAGG ATTTGGCCGA TATGCTAGCA 
GCTCAACTGG GAATTAAATA TGAAATACGC CTGGTGCAAG ATGGCAACTA TGGTGCAGAG 
AATCAGTATG CTCCTGGCGG TTGGGATGGC ATGGTGGGTG AGCTGATTCG CAAGGAGGCG 
GATATAGCCA TATCCGCAAT GACTATTACA GCCGAAAGAG AACGGGTTAT AGACTTTAGC 
AAACCCTTTA TGACCCTGGG AATATCCATT ATGATAAAGA AGCCCGTGAA ACAGACACCA 
GGAGTGTTTA GTTTTCTTAA TCCCCTATCC CAGGAAATTT GGATAAGCGT GATTCTCAGC 
TATGTGGGTG TTAGTTTTGT TCTGTACTTT GTTACACGTT TCCCACCCTA TGAGTGGCGG 
ATTGTGAGGC GTCCTCAAGC GGATTCCACT GCCCAGCAAC CACCGGGAAT TATTGGAGGT 
GCCACTTTGA GTGAACCACA AGCGCATGTG CCTCCAGTTC CACCTAATGA GTTCACTATG 
CTGAACTCCT TTTGGTATTC CTTGGCTGCT TTTATGCAGC AGGGATGCGA TATTACACCT 
CCTTCGATTG CCGGAAGGAT AGCAGCTGCC GTCTGGTGGT TCTTCACTAT CATATTAATA 
TCCTCGTATA CCGCCAATTT GGCCGCTTTT CTCACAGTGG AGCGTATGGT GGCGCCCATC 
AAAACGCCCG AGGACTTGAC CATGCAAACG GATGTGAACT ATGGTACCCT GTTATACGGC 
TCCACTTGGG AATTCTTTAG GCGTTCACAA ATCGGTCTGC ACAATAAAAT GTGGGAGTAT 
ATGAATGCCA ATCAACACCA TTCGGTGCAC ACCTATGATG AGGGTATTCG TAGGGTGAGG 
CAATCCAAGG GCAAGTATGC CCTACTGGTG GAGTCACCCA AAAACGAGTA TGTAAATGCT 
AGGCCTCCGT GCGACACAAT GAAAGTGGGA CGCAATATCG ATACGAAGGG ATTTGGCGTG 
GCCACACCCA TAGGATCGCC ACTGAGAAAA CGCTTAAACG AGGCCGTGCT AACGCTAAAG 
GAGAACGGTG AGCTGTTGCG GATTAGGAAT AAATGGTGGT TCGACAAGAC CGAGTGCAAT 
CTGGACCAGG AGACCTCCAC ACCGAACGAG CTCTCGCTAA GCAATGTGGC CGGGATCTAC 
TACATCCTAA TCGGAGGCCT GCTCCTGGCC GTGATTGTGG CCATCATGGA GTTCTTCTGC 
CGCAACAAAA CCCCGCAGCT AAAGTCGCCA GGATCCAATG GATCCGCTGG TGGAGTGCCC 
GGAATGCTGG CCTCCTCAAC ATATCAAAGG GATTCCCTTT CCGATGCGAT TATGCATTCG 
CAGGCCAAGT TGGCGATGCA GGCGAGCAGC GAGTATGACG AGCGATTGGT GGGCGTTGAG 
TTGGCCTCGA ACGTGCGGTA TCAGTACAGC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GluRIA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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